pycom15g32820

Removes the phosphate from trehalose 6-phosphate to produce free trehalose

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0006485
Physical Location & Seq
Forward (+)
32060928 .. 32064114
3187 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1332 bp
ATGATCAAAGCCGACTCGACTGCGCGTGTGGTTCGATTCTCGACTTCTTCTCTCTTTCCCTCAGGTTTTTTGTTTCTATTTAAACCCTCCCACCCTGCCTTCTGTTTCATCATCTCCCAAACAACCCACAAACCTCCTCTGTTCTTCTCTTCCTCTGTTTTTTCTCTTCCTCAAATTCTTCGAAACCTTCGAGGCAGAAAAATGACCAACCAAAATGTGGTTGTTTCTGATGCCAAAGGCGTGGCTATTACAGTCACGGTTGCTGCAAAGCAGAATATGTTTTCACAATCGGTGCCAAAACCGATGACTGAGGCAGGAAGAGGAGGAGGATGCTTCACCATTCCCATGAACATCAAGGTTTTGAATGTTGACAAAGAAGGGGGAGCAGCCCGAGTCAATGCTTGGGTTGACTCCTTGAGAGCTTCTTCTCCGCCTCGTGTCAAATCCGCTGCCACTCCTCAGTCTCTTTCTGAAACAGTGGATAATAACTCTTGGAGTAGTCGCCATCCATCAGCTATGAGCATGTTCGAGCAGATAACAAATGCTTCAAAGGGCAAGCAGATTGTATTGTTTCTTGACTACGATGGCACCCTCTCCCCAATTGTGGAAGACCCAGATCGTGCTTTCATGTCCAATGAGATGAGAAAGGCAGTGAAGGCCGCTGCTAGATACTTTCCTACAGCAATTGTTAGCGGGAGGTGCAGAGACAAGGTTTACAGCTTTGTAAAATTAGGAGAGCTTTATTATGCAGGCAGCCATGGGATGGACATCAAGGGACCATCCAAAAGTAGCAAATATAAGAATGGTAATCAAGCAGTTCTCTTCCAACCGGCCAGTGAGTTTGTACCAATGATTGATGAGGTGTACAAAATTTTGTTGGAGAAAACCAGATCCATCTCAGGAGCTAGGGTGGAAAATAATAAGTTTTGCTTATCTGTACACTTCCGTTGTGTTGATGAGAAGAGTTGGGCAGCTTTGGCAGAGCAAGTTAGACTTGTTCTCAATGAGTATCCGAAGCTCAGGTTGACTCAAGGGAGGAAGGTATTAGAGATCCGCCCAACCATCAAATGGGACAAAGGCAAAGCTCTTGAATTCCTGCTAGAGTCATTAGGATATGCGAACTCAAACGAAGTACTACCAGTCTATATTGGAGATGATCGAACAGACGAGGACGCCTTCAAGGTGCTACGCGATAGAGGACAAGGATTTGGTATTCTAGTTTCTAAAGTTCCAAAAGAAACAAATGCGTCTTACACTTTACAAGAACCAGCGGAGGTTAAGGATTTTTTGCGGCGTTTGGTGAAATGGAAAACCAGCAAACTAACTAGCTAG

Protein Analysis

444

Amino Acids

49.31

Weight (kDa)

9.7

Isoelectric Point (pI)

33.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trehalose_PPase PF02358 191 - 423 1e-72 Trehalose-phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 292, 587
AccB7I CCANNNNNTGG 3 cut(s) 217, 763, 1068
AccII CGCG 2 cut(s) 25, 1191
AciI CCGC 7 cut(s) 431, 447, 660, 693, 1054, 1271, 1291
AclWI GGATC 2 cut(s) 885, 1045
AcoI YGGCCR 1 cut(s) 831
AcsI RAATTY 3 cut(s) 174, 870, 1091
AcyI GRCGYC 1 cut(s) 1173
AfaI GTAC 4 cut(s) 846, 866, 939, 1134
AfiI CCNNNNNNNGG 4 cut(s) 217, 604, 763, 1068
AgsI TTSAA 4 cut(s) 364, 549, 1091, 1180
AluBI AGCT 9 cut(s) 422, 515, 720, 739, 905, 974, 1018, 1085, 1329
AluI AGCT 9 cut(s) 422, 515, 720, 739, 905, 974, 1018, 1085, 1329
Alw26I GTCTC 2 cut(s) 468, 699
AlwI GGATC 2 cut(s) 885, 1045
Ama87I CYCGRG 1 cut(s) 390
AoxI GGCC 2 cut(s) 657, 831
ApeKI GCWGC 6 cut(s) 263, 386, 449, 662, 753, 971
ApoI RAATTY 3 cut(s) 174, 870, 1091
AspLEI GCGC 1 cut(s) 25
AspS9I GGNCC 1 cut(s) 776
AsuHPI GGTGA 2 cut(s) 328, 1312
AsuII TTCGAA 1 cut(s) 181
AvaI CYCGRG 1 cut(s) 390
AvaII GGWCC 1 cut(s) 776
AxyI CCTNAGG 1 cut(s) 61
BanI GGYRCC 2 cut(s) 292, 587
BauI CACGAG 1 cut(s) 435
BbsI GAAGAC 1 cut(s) 615
BbvI GCAGC 6 cut(s) 250, 398, 436, 649, 765, 983
BccI CCATC 7 cut(s) 513, 517, 578, 757, 787, 902, 1070
BcgI CGANNNNNNTGC 1 cut(s) 36
BciVI GTATCC 1 cut(s) 1020
BclI TGATCA 1 cut(s) 3
BcoDI GTCTC 2 cut(s) 468, 699
BfaI CTAG 6 cut(s) 666, 906, 1100, 1217, 1326, 1330
BfmI CTRYAG 1 cut(s) 678
BfuI GTATCC 1 cut(s) 1020
BisI GCNGC 8 cut(s) 264, 387, 450, 660, 663, 754, 972, 1292
BlsI GCNGC 8 cut(s) 265, 388, 451, 661, 664, 755, 973, 1293
BmcAI AGTACT 1 cut(s) 1134
Bme18I GGWCC 1 cut(s) 776
BmeT110I CYCGRG 1 cut(s) 390
BmgT120I GGNCC 1 cut(s) 776
BmiI GGNNCC 3 cut(s) 294, 589, 777
BmsI GCATC 2 cut(s) 220, 320
BpiI GAAGAC 1 cut(s) 615
Bpu10I CCTNAGC 1 cut(s) 1019
Bpu14I TTCGAA 1 cut(s) 181
BpuEI CTTGAG 2 cut(s) 436, 1014
BsaBI GATNNNNATC 1 cut(s) 767
BsaHI GRCGYC 1 cut(s) 1173
BsaJI CCNNGG 1 cut(s) 757
BsaXI ACNNNNNCTCC 2 cut(s) 894, 924
Bsc4I CCNNNNNNNGG 4 cut(s) 217, 604, 763, 1068
Bse118I RCCGGY 1 cut(s) 829
Bse1I ACTGG 2 cut(s) 834, 1139
Bse21I CCTNAGG 1 cut(s) 61
Bse8I GATNNNNATC 1 cut(s) 767
BseDI CCNNGG 1 cut(s) 757
BseGI GGATG 4 cut(s) 335, 505, 768, 779
BseJI GATNNNNATC 1 cut(s) 767
BseLI CCNNNNNNNGG 4 cut(s) 217, 604, 763, 1068
BseMII CTCAG 5 cut(s) 75, 300, 473, 912, 1033
BseNI ACTGG 2 cut(s) 834, 1139
BseRI GAGGAG 4 cut(s) 126, 336, 339, 447
BseXI GCAGC 6 cut(s) 250, 398, 436, 649, 765, 983
BsgI GTGCAG 1 cut(s) 721
Bsh1236I CGCG 2 cut(s) 25, 1191
BshFI GGCC 2 cut(s) 659, 833
BshNI GGYRCC 2 cut(s) 292, 587
BsiHKCI CYCGRG 1 cut(s) 390
BsiSI CCGG 1 cut(s) 830
BslFI GGGAC 2 cut(s) 789, 1085
BslI CCNNNNNNNGG 4 cut(s) 217, 604, 763, 1068
BsmAI GTCTC 2 cut(s) 468, 699
BsmFI GGGAC 2 cut(s) 789, 1085
BsnI GGCC 2 cut(s) 659, 833
BsoBI CYCGRG 1 cut(s) 390
Bsp119I TTCGAA 1 cut(s) 181
Bsp1407I TGTACA 2 cut(s) 864, 937
Bsp143I GATC 5 cut(s) 3, 616, 890, 1050, 1156
Bsp19I CCATGG 1 cut(s) 757
BspACI CCGC 7 cut(s) 431, 447, 660, 693, 1054, 1271, 1291
BspANI GGCC 2 cut(s) 659, 833
BspCNI CTCAG 5 cut(s) 74, 301, 472, 911, 1032
BspFNI CGCG 2 cut(s) 25, 1191
BspLI GGNNCC 3 cut(s) 294, 589, 777
BspPI GGATC 2 cut(s) 885, 1045
BspT104I TTCGAA 1 cut(s) 181
BspT107I GGYRCC 2 cut(s) 292, 587
BsrFI RCCGGY 1 cut(s) 829
BsrGI TGTACA 2 cut(s) 864, 937
BsrI ACTGG 2 cut(s) 834, 1139
BssAI RCCGGY 1 cut(s) 829
BssECI CCNNGG 1 cut(s) 757
BssMI GATC 5 cut(s) 3, 616, 890, 1050, 1156
BssNI GRCGYC 1 cut(s) 1173
BssSI CACGAG 1 cut(s) 435
BssT1I CCWWGG 1 cut(s) 757
Bst2BI CACGAG 1 cut(s) 435
Bst4CI ACNGT 3 cut(s) 253, 259, 478
Bst6I CTCTTC 5 cut(s) 154, 171, 313, 827, 956
BstACI GRCGYC 1 cut(s) 1173
BstAUI TGTACA 2 cut(s) 864, 937
BstBI TTCGAA 1 cut(s) 181
BstC8I GCNNGC 2 cut(s) 557, 751
BstDEI CTNAG 5 cut(s) 61, 309, 459, 898, 1019
BstDSI CCRYGG 1 cut(s) 757
BstF5I GGATG 4 cut(s) 335, 505, 768, 779
BstFNI CGCG 2 cut(s) 25, 1191
BstHHI GCGC 1 cut(s) 25
BstKTI GATC 5 cut(s) 6, 619, 893, 1053, 1159
BstMAI GTCTC 2 cut(s) 468, 699
BstMBI GATC 5 cut(s) 3, 616, 890, 1050, 1156
BstMWI GCNNNNNNNGC 3 cut(s) 656, 699, 977
BstNSI RCATGY 1 cut(s) 526
BstSFI CTRYAG 1 cut(s) 678
BstUI CGCG 2 cut(s) 25, 1191
BstV1I GCAGC 6 cut(s) 250, 398, 436, 649, 765, 983
BstV2I GAAGAC 1 cut(s) 615
BstX2I RGATCY 2 cut(s) 890, 1050
BstXI CCANNNNNNTGG 1 cut(s) 241
BstYI RGATCY 2 cut(s) 890, 1050
Bsu36I CCTNAGG 1 cut(s) 61
BsuI GTATCC 1 cut(s) 1020
BsuRI GGCC 2 cut(s) 659, 833
BtgI CCRYGG 1 cut(s) 757
BtsCI GGATG 4 cut(s) 335, 505, 768, 779
BtsI GCAGTG 1 cut(s) 657
BtsIMutI CAGTG 3 cut(s) 483, 657, 841
Cac8I GCNNGC 2 cut(s) 557, 751
CfoI GCGC 1 cut(s) 25
Cfr10I RCCGGY 1 cut(s) 829
Cfr13I GGNCC 1 cut(s) 776
CseI GACGC 2 cut(s) 1181, 1236
Csp6I GTAC 4 cut(s) 845, 865, 938, 1133
CviAII CATG 4 cut(s) 346, 523, 628, 758
CviQI GTAC 4 cut(s) 845, 865, 938, 1133
DdeI CTNAG 5 cut(s) 61, 309, 459, 898, 1019
DpnI GATC 5 cut(s) 5, 618, 892, 1052, 1158
DpnII GATC 5 cut(s) 3, 616, 890, 1050, 1156
DraI TTTAAA 1 cut(s) 82
EaeI YGGCCR 1 cut(s) 831
Eam1104I CTCTTC 5 cut(s) 154, 171, 313, 827, 956
EarI CTCTTC 5 cut(s) 154, 171, 313, 827, 956
EciI GGCGGA 2 cut(s) 420, 1043
Eco130I CCWWGG 1 cut(s) 757
Eco47I GGWCC 1 cut(s) 776
Eco81I CCTNAGG 1 cut(s) 61
Eco88I CYCGRG 1 cut(s) 390
EcoRI GAATTC 1 cut(s) 1091
EcoT14I CCWWGG 1 cut(s) 757
ErhI CCWWGG 1 cut(s) 757
FaeI CATG 4 cut(s) 349, 526, 631, 761
FalI AAGNNNNNCTT 4 cut(s) 914, 946, 978, 1010
FaqI GGGAC 2 cut(s) 789, 1085
FatI CATG 4 cut(s) 345, 522, 627, 757
FauI CCCGC 1 cut(s) 686
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 8 cut(s) 264, 387, 450, 660, 663, 754, 972, 1292
FokI GGATG 4 cut(s) 342, 492, 766, 775
Fsp4HI GCNGC 8 cut(s) 264, 387, 450, 660, 663, 754, 972, 1292
FspBI CTAG 6 cut(s) 666, 906, 1100, 1217, 1326, 1330
GlaI GCGC 1 cut(s) 24
GluI GCNGC 8 cut(s) 264, 387, 450, 660, 663, 754, 972, 1292
HaeIII GGCC 2 cut(s) 659, 833
HapII CCGG 1 cut(s) 830
HgaI GACGC 2 cut(s) 1181, 1236
HhaI GCGC 1 cut(s) 25
Hin1I GRCGYC 1 cut(s) 1173
Hin1II CATG 4 cut(s) 349, 526, 631, 761
Hin6I GCGC 1 cut(s) 23
HinP1I GCGC 1 cut(s) 23
HincII GTYRAC 3 cut(s) 370, 409, 1026
HindII GTYRAC 3 cut(s) 370, 409, 1026
HinfI GANTC 6 cut(s) 14, 36, 393, 410, 1027, 1103
HpaII CCGG 1 cut(s) 830
HphI GGTGA 2 cut(s) 328, 1312
Hpy166II GTNNAC 6 cut(s) 370, 409, 715, 865, 940, 1026
Hpy188I TCNGA 3 cut(s) 229, 472, 1014
Hpy188III TCNNGA 4 cut(s) 40, 575, 900, 1088
Hpy8I GTNNAC 6 cut(s) 370, 409, 715, 865, 940, 1026
HpyAV CCTTC 6 cut(s) 109, 197, 371, 649, 1033, 1186
HpyCH4III ACNGT 3 cut(s) 253, 259, 478
HpyCH4V TGCA 3 cut(s) 266, 702, 749
HpyF10VI GCNNNNNNNGC 3 cut(s) 656, 699, 977
HpyF3I CTNAG 5 cut(s) 61, 309, 459, 898, 1019
Hsp92I GRCGYC 1 cut(s) 1173
Hsp92II CATG 4 cut(s) 349, 526, 631, 761
HspAI GCGC 1 cut(s) 23
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 5 cut(s) 3, 616, 890, 1050, 1156
LmnI GCTCC 2 cut(s) 383, 902
Lsp1109I GCAGC 6 cut(s) 250, 398, 436, 649, 765, 983
LweI GCATC 2 cut(s) 220, 320
MaeI CTAG 6 cut(s) 666, 906, 1100, 1217, 1326, 1330
MaeIII GTNAC 1 cut(s) 253
MalI GATC 5 cut(s) 5, 618, 892, 1052, 1158
MboI GATC 5 cut(s) 3, 616, 890, 1050, 1156
MfeI CAATTG 2 cut(s) 600, 684
MflI RGATCY 2 cut(s) 890, 1050
MluCI AATT 6 cut(s) 174, 600, 684, 728, 870, 1091
MlyI GAGTC 5 cut(s) 8, 402, 404, 1021, 1112
MmeI TCCRAC 2 cut(s) 850, 858
MseI TTAA 2 cut(s) 81, 1278
MslI CAYNNNNRTG 1 cut(s) 344
MspA1I CMGCKG 3 cut(s) 449, 662, 1271
MspI CCGG 1 cut(s) 830
MunI CAATTG 2 cut(s) 600, 684
MvnI CGCG 2 cut(s) 25, 1191
MwoI GCNNNNNNNGC 3 cut(s) 656, 699, 977
NcoI CCATGG 1 cut(s) 757
NdeII GATC 5 cut(s) 3, 616, 890, 1050, 1156
NlaIII CATG 4 cut(s) 349, 526, 631, 761
NlaIV GGNNCC 3 cut(s) 294, 589, 777
NmuCI GTSAC 1 cut(s) 253
NspI RCATGY 1 cut(s) 526
NspV TTCGAA 1 cut(s) 181
PcsI WCGNNNNNNNCGW 1 cut(s) 187
PfeI GAWTC 1 cut(s) 36
PflMI CCANNNNNTGG 3 cut(s) 217, 763, 1068
PkrI GCNGC 8 cut(s) 265, 388, 451, 661, 664, 755, 973, 1293
PleI GAGTC 5 cut(s) 8, 401, 404, 1021, 1111
PpsI GAGTC 5 cut(s) 8, 401, 404, 1021, 1111
PspN4I GGNNCC 3 cut(s) 294, 589, 777
PspPI GGNCC 1 cut(s) 776
PsuI RGATCY 2 cut(s) 890, 1050
RsaI GTAC 4 cut(s) 846, 866, 939, 1134
RsaNI GTAC 4 cut(s) 845, 865, 938, 1133
RseI CAYNNNNRTG 1 cut(s) 344
SaqAI TTAA 2 cut(s) 81, 1278
SatI GCNGC 8 cut(s) 264, 387, 450, 660, 663, 754, 972, 1292
Sau3AI GATC 5 cut(s) 3, 616, 890, 1050, 1156
Sau96I GGNCC 1 cut(s) 776
ScaI AGTACT 1 cut(s) 1134
SchI GAGTC 5 cut(s) 8, 402, 404, 1021, 1112
SfaNI GCATC 2 cut(s) 220, 320
SfcI CTRYAG 1 cut(s) 678
SfuI TTCGAA 1 cut(s) 181
SinI GGWCC 1 cut(s) 776
SmiMI CAYNNNNRTG 1 cut(s) 344
SmlI CTYRAG 2 cut(s) 415, 1029
SmoI CTYRAG 2 cut(s) 415, 1029
Sse9I AATT 6 cut(s) 174, 600, 684, 728, 870, 1091
SsiI CCGC 7 cut(s) 431, 447, 660, 693, 1054, 1271, 1291
SspMI CTAG 6 cut(s) 666, 906, 1100, 1217, 1326, 1330
StyI CCWWGG 1 cut(s) 757
TaaI ACNGT 3 cut(s) 253, 259, 478
TaqI TCGA 7 cut(s) 17, 34, 41, 181, 190, 528, 1159
TasI AATT 6 cut(s) 174, 600, 684, 728, 870, 1091
TatI WGTACW 3 cut(s) 864, 937, 1132
TauI GCSGC 2 cut(s) 662, 1294
TfiI GAWTC 1 cut(s) 36
Tru1I TTAA 2 cut(s) 81, 1278
Tru9I TTAA 2 cut(s) 81, 1278
TscAI CASTG 3 cut(s) 483, 657, 841
TseFI GTSAC 1 cut(s) 253
TseI GCWGC 6 cut(s) 263, 386, 449, 662, 753, 971
Tsp45I GTSAC 1 cut(s) 253
TspDTI ATGAA 3 cut(s) 97, 362, 616
TspGWI ACGGA 1 cut(s) 935
TspRI CASTG 3 cut(s) 483, 657, 841
Van91I CCANNNNNTGG 3 cut(s) 217, 763, 1068
VpaK11BI GGWCC 1 cut(s) 776
XapI RAATTY 3 cut(s) 174, 870, 1091
XceI RCATGY 1 cut(s) 526
XcmI CCANNNNNNNNNTGG 2 cut(s) 214, 1065
XspI CTAG 6 cut(s) 666, 906, 1100, 1217, 1326, 1330
ZrmI AGTACT 1 cut(s) 1134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.