pycom15g33630

Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis- dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0004542
Physical Location & Seq
Reverse (-)
33084263 .. 33091351
7089 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1197 bp
ATGGCACTTCGTTTCGAGGTTTTGGGGAGGTTTAACCGTGCTCGTGCAGCTAGACTGACCCTGCCTCACTTCGTGTGCCAGACACCGCTTTTCATGCCCGTTGGAACGCAAGGAACTATAAAAGGGTTGACAACTGAACAGCTTGAAGATATTGGTGCTCAAATAATACTTGGGAATACGTATCATTTGGCACTTCGTCCAACCTCCGAGCTTCTTGATGAGTTGGGAGGTCTCCATAAATTTATGAACTGGCCAAGAGCATTGCTTACTGATTCTGGTGGCTTTCAGATGGTATCATTATTGCATTTAGCTGACATCACTGAAGAAGGTGTAACATTTCAGTCACCAGTTGATGGAAAGCCAATGCTCTTGACACCTGAGGAGTCAATTCAAATCCAAAACAGAATTGGAGCAGATATTATTATGGCCCTCGATGATGTAGTCAAAACCACCACCACTGGTCCAAGAGTTGAGGAGGCTATGTATCGCACTATACGATGGATAGATAGATGCATTGAAGCTCACAAGAGACCAACGGAGCAGAACTTATTTGGTATTGTACAAGGTGGCTTAGATCCTGTACTAAGAGATATATGTGTTAGGGGCTTGGTGAATCGAAATTTACCCGGCTATGCTATTGGCGGTCTTGCTGGCGGTGAAAGTAAGGATTCATTTTGGCGTGTGGTTGCACAGTGCACTGCTGCATTGCCTGAGGAGAAACCACGATACGTTATGGGTGTTGGTTATCCGCTGGATATTGTAGTTTGCAGTGCTCTAGGTGCTGATATGTATGACTGCGTTTATCCTACACGTACTGCTCGCTTTGGTACAGCTCTTGTACCTGAGGGAGTGCTGAAACTCAAACACAAGGCGATGGCTGAAGATACCCGTCCTGTTGATCCTACATGTGATTGTATGGTCTGCAAAAATTATACTAGAGCTTACATTCATTGCCTGGTTACAAAAGATGCTATGGGATCTCAGCTTCTATCATATCACAATTTATATTACATGATGAAGCTAAGTAGAAATCTGCACTCTTCAATAATCGAAGGAAGGTTTCCAGAGTTTGTATGCAACTTTCTGCAACAAATGTTTCCGAAAGGTGATGTGCCTGAGTGGGTGTGTGATGCCATGGAGGTTGCTGGAATCGATATTTCTTCCTGCTGCACTCCATTTTCATCATCCCAAGATTAG

Protein Analysis

399

Amino Acids

44.19

Weight (kDa)

5.77

Isoelectric Point (pI)

46.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TGT PF01702 13 - 365 1.6e-154 Queuine tRNA-ribosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 353
AciI CCGC 4 cut(s) 86, 642, 654, 749
AclWI GGATC 3 cut(s) 569, 893, 985
AcoI YGGCCR 1 cut(s) 251
AcsI RAATTY 2 cut(s) 239, 619
AcuI CTGAAG 2 cut(s) 342, 900
AdeI CACNNNGTG 1 cut(s) 73
AfaI GTAC 5 cut(s) 561, 582, 814, 829, 840
AfiI CCNNNNNNNGG 1 cut(s) 353
AflIII ACRYGT 2 cut(s) 809, 905
AgsI TTSAA 4 cut(s) 146, 392, 518, 1044
AjnI CCWGG 1 cut(s) 954
AluBI AGCT 9 cut(s) 50, 142, 211, 311, 521, 833, 941, 985, 1021
AluI AGCT 9 cut(s) 50, 142, 211, 311, 521, 833, 941, 985, 1021
Alw21I GWGCWC 4 cut(s) 43, 160, 698, 775
Alw26I GTCTC 2 cut(s) 236, 523
Alw44I GTGCAC 1 cut(s) 694
AlwI GGATC 3 cut(s) 569, 893, 985
AoxI GGCC 2 cut(s) 251, 426
ApaLI GTGCAC 1 cut(s) 694
ApeKI GCWGC 3 cut(s) 47, 701, 1167
ApoI RAATTY 2 cut(s) 239, 619
AspS9I GGNCC 2 cut(s) 427, 461
AsuC2I CCSGG 1 cut(s) 627
AsuHPI GGTGA 4 cut(s) 336, 622, 668, 1118
AvaII GGWCC 1 cut(s) 461
AxyI CCTNAGG 3 cut(s) 378, 711, 843
BaeGI GKGCMC 1 cut(s) 698
BalI TGGCCA 1 cut(s) 253
BauI CACGAG 1 cut(s) 42
Bbv12I GWGCWC 4 cut(s) 43, 160, 698, 775
BbvI GCAGC 3 cut(s) 59, 688, 1154
BccI CCATC 4 cut(s) 283, 347, 492, 868
BciT130I CCWGG 1 cut(s) 956
BcnI CCSGG 1 cut(s) 627
BcoDI GTCTC 2 cut(s) 236, 523
BfaI CTAG 3 cut(s) 51, 776, 936
BisI GCNGC 3 cut(s) 48, 702, 1168
BlsI GCNGC 3 cut(s) 49, 703, 1169
Bme1390I CCNGG 2 cut(s) 627, 956
Bme18I GGWCC 1 cut(s) 461
BmgT120I GGNCC 2 cut(s) 427, 461
BmrFI CCNGG 2 cut(s) 627, 956
BmsI GCATC 3 cut(s) 500, 958, 1120
BpuMI CCSGG 1 cut(s) 627
Bsa29I ATCGAT 1 cut(s) 1152
BsaAI YACGTR 2 cut(s) 180, 812
BsaI GGTCTC 2 cut(s) 236, 523
BsaJI CCNNGG 1 cut(s) 1134
BsaXI ACNNNNNCTCC 2 cut(s) 467, 497
Bsc4I CCNNNNNNNGG 1 cut(s) 353
Bse1I ACTGG 3 cut(s) 254, 347, 463
Bse21I CCTNAGG 3 cut(s) 378, 711, 843
Bse3DI GCAATG 3 cut(s) 260, 704, 949
BseBI CCWGG 1 cut(s) 956
BseCI ATCGAT 1 cut(s) 1152
BseDI CCNNGG 1 cut(s) 1134
BseGI GGATG 1 cut(s) 1184
BseLI CCNNNNNNNGG 1 cut(s) 353
BseMI GCAATG 3 cut(s) 260, 704, 949
BseMII CTCAG 5 cut(s) 369, 702, 834, 995, 1107
BseNI ACTGG 3 cut(s) 254, 347, 463
BseRI GAGGAG 3 cut(s) 395, 488, 728
BseSI GKGCMC 1 cut(s) 698
BseXI GCAGC 3 cut(s) 59, 688, 1154
BsgI GTGCAG 3 cut(s) 66, 1019, 1153
BshFI GGCC 2 cut(s) 253, 428
BshVI ATCGAT 1 cut(s) 1152
BsiHKAI GWGCWC 4 cut(s) 43, 160, 698, 775
BsiSI CCGG 1 cut(s) 627
BslI CCNNNNNNNGG 1 cut(s) 353
BsmAI GTCTC 2 cut(s) 236, 523
BsnI GGCC 2 cut(s) 253, 428
Bso31I GGTCTC 2 cut(s) 236, 523
Bsp1286I GDGCHC 4 cut(s) 43, 160, 698, 775
Bsp1407I TGTACA 1 cut(s) 559
Bsp143I GATC 3 cut(s) 574, 898, 977
Bsp19I CCATGG 1 cut(s) 1134
BspACI CCGC 4 cut(s) 86, 642, 654, 749
BspANI GGCC 2 cut(s) 253, 428
BspCNI CTCAG 5 cut(s) 370, 703, 835, 994, 1108
BspDI ATCGAT 1 cut(s) 1152
BspPI GGATC 3 cut(s) 569, 893, 985
BspTNI GGTCTC 2 cut(s) 236, 523
BsrDI GCAATG 3 cut(s) 260, 704, 949
BsrGI TGTACA 1 cut(s) 559
BsrI ACTGG 3 cut(s) 254, 347, 463
BssECI CCNNGG 1 cut(s) 1134
BssMI GATC 3 cut(s) 574, 898, 977
BssSI CACGAG 1 cut(s) 42
BssT1I CCWWGG 1 cut(s) 1134
Bst2BI CACGAG 1 cut(s) 42
Bst2UI CCWGG 1 cut(s) 956
Bst4CI ACNGT 2 cut(s) 38, 693
Bst6I CTCTTC 1 cut(s) 1045
BstAUI TGTACA 1 cut(s) 559
BstBAI YACGTR 2 cut(s) 180, 812
BstC8I GCNNGC 2 cut(s) 652, 820
BstDEI CTNAG 8 cut(s) 378, 571, 584, 711, 843, 981, 1022, 1116
BstDSI CCRYGG 1 cut(s) 1134
BstF5I GGATG 1 cut(s) 1184
BstKTI GATC 3 cut(s) 577, 901, 980
BstMAI GTCTC 2 cut(s) 236, 523
BstMBI GATC 3 cut(s) 574, 898, 977
BstMWI GCNNNNNNNGC 3 cut(s) 47, 94, 779
BstNI CCWGG 1 cut(s) 956
BstNSI RCATGY 1 cut(s) 909
BstSCI CCNGG 2 cut(s) 625, 954
BstSLI GKGCMC 1 cut(s) 698
BstSNI TACGTA 1 cut(s) 180
BstV1I GCAGC 3 cut(s) 59, 688, 1154
BstX2I RGATCY 2 cut(s) 574, 977
BstYI RGATCY 2 cut(s) 574, 977
Bsu15I ATCGAT 1 cut(s) 1152
Bsu36I CCTNAGG 3 cut(s) 378, 711, 843
BsuRI GGCC 2 cut(s) 253, 428
BsuTUI ATCGAT 1 cut(s) 1152
BtgI CCRYGG 1 cut(s) 1134
BtgZI GCGATG 1 cut(s) 887
BtsCI GGATG 1 cut(s) 1184
BtsI GCAGTG 2 cut(s) 696, 775
BtsIMutI CAGTG 5 cut(s) 318, 456, 696, 698, 775
Cac8I GCNNGC 2 cut(s) 652, 820
Cfr13I GGNCC 2 cut(s) 427, 461
ClaI ATCGAT 1 cut(s) 1152
Csp6I GTAC 5 cut(s) 560, 581, 813, 828, 839
CviAII CATG 4 cut(s) 94, 906, 1012, 1135
CviQI GTAC 5 cut(s) 560, 581, 813, 828, 839
DdeI CTNAG 8 cut(s) 378, 571, 584, 711, 843, 981, 1022, 1116
DpnI GATC 3 cut(s) 576, 900, 979
DpnII GATC 3 cut(s) 574, 898, 977
DraIII CACNNNGTG 1 cut(s) 73
EaeI YGGCCR 1 cut(s) 251
Eam1104I CTCTTC 1 cut(s) 1045
EarI CTCTTC 1 cut(s) 1045
Eco105I TACGTA 1 cut(s) 180
Eco130I CCWWGG 1 cut(s) 1134
Eco31I GGTCTC 2 cut(s) 236, 523
Eco47I GGWCC 1 cut(s) 461
Eco57I CTGAAG 2 cut(s) 342, 900
Eco81I CCTNAGG 3 cut(s) 378, 711, 843
EcoRII CCWGG 1 cut(s) 954
EcoT14I CCWWGG 1 cut(s) 1134
EcoT22I ATGCAT 1 cut(s) 515
ErhI CCWWGG 1 cut(s) 1134
FaeI CATG 4 cut(s) 97, 909, 1015, 1138
FatI CATG 4 cut(s) 93, 905, 1011, 1134
Fnu4HI GCNGC 3 cut(s) 48, 702, 1168
FokI GGATG 1 cut(s) 1171
Fsp4HI GCNGC 3 cut(s) 48, 702, 1168
FspBI CTAG 3 cut(s) 51, 776, 936
GluI GCNGC 3 cut(s) 48, 702, 1168
HaeIII GGCC 2 cut(s) 253, 428
HapII CCGG 1 cut(s) 627
Hin1II CATG 4 cut(s) 97, 909, 1015, 1138
HincII GTYRAC 1 cut(s) 129
HindII GTYRAC 1 cut(s) 129
HinfI GANTC 5 cut(s) 272, 383, 613, 668, 1149
HpaII CCGG 1 cut(s) 627
HphI GGTGA 4 cut(s) 336, 622, 668, 1118
Hpy166II GTNNAC 2 cut(s) 129, 696
Hpy188I TCNGA 3 cut(s) 208, 288, 1101
Hpy188III TCNNGA 3 cut(s) 215, 370, 1064
Hpy8I GTNNAC 2 cut(s) 129, 696
HpyAV CCTTC 3 cut(s) 320, 1046, 1050
HpyCH4III ACNGT 2 cut(s) 38, 693
HpyCH4IV ACGT 3 cut(s) 179, 729, 811
HpyF10VI GCNNNNNNNGC 3 cut(s) 47, 94, 779
HpyF3I CTNAG 8 cut(s) 378, 571, 584, 711, 843, 981, 1022, 1116
HpySE526I ACGT 3 cut(s) 179, 729, 811
Hsp92II CATG 4 cut(s) 97, 909, 1015, 1138
Kzo9I GATC 3 cut(s) 574, 898, 977
LmnI GCTCC 2 cut(s) 410, 538
Lsp1109I GCAGC 3 cut(s) 59, 688, 1154
LweI GCATC 3 cut(s) 500, 958, 1120
MaeI CTAG 3 cut(s) 51, 776, 936
MaeII ACGT 3 cut(s) 179, 729, 811
MaeIII GTNAC 3 cut(s) 331, 342, 958
MalI GATC 3 cut(s) 576, 900, 979
MboI GATC 3 cut(s) 574, 898, 977
MboII GAAGA 5 cut(s) 158, 335, 893, 1032, 1152
MflI RGATCY 2 cut(s) 574, 977
MhlI GDGCHC 4 cut(s) 43, 160, 698, 775
MlsI TGGCCA 1 cut(s) 253
MluCI AATT 6 cut(s) 239, 387, 405, 619, 928, 1000
MluNI TGGCCA 1 cut(s) 253
MlyI GAGTC 1 cut(s) 392
MmeI TCCRAC 2 cut(s) 82, 224
Mox20I TGGCCA 1 cut(s) 253
Mph1103I ATGCAT 1 cut(s) 515
MscI TGGCCA 1 cut(s) 253
MseI TTAA 1 cut(s) 33
Msp20I TGGCCA 1 cut(s) 253
MspA1I CMGCKG 1 cut(s) 751
MspI CCGG 1 cut(s) 627
MspR9I CCNGG 2 cut(s) 627, 956
MvaI CCWGG 1 cut(s) 956
MwoI GCNNNNNNNGC 3 cut(s) 47, 94, 779
NciI CCSGG 1 cut(s) 627
NcoI CCATGG 1 cut(s) 1134
NdeII GATC 3 cut(s) 574, 898, 977
NlaIII CATG 4 cut(s) 97, 909, 1015, 1138
NmuCI GTSAC 1 cut(s) 342
NsiI ATGCAT 1 cut(s) 515
NspI RCATGY 1 cut(s) 909
PciI ACATGT 1 cut(s) 905
PcsI WCGNNNNNNNCGW 1 cut(s) 493
PfeI GAWTC 4 cut(s) 272, 613, 668, 1149
PflMI CCANNNNNTGG 1 cut(s) 353
PkrI GCNGC 3 cut(s) 49, 703, 1169
PleI GAGTC 1 cut(s) 391
PpsI GAGTC 1 cut(s) 391
Ppu21I YACGTR 2 cut(s) 180, 812
PscI ACATGT 1 cut(s) 905
Psp6I CCWGG 1 cut(s) 954
PspGI CCWGG 1 cut(s) 954
PspPI GGNCC 2 cut(s) 427, 461
PsuI RGATCY 2 cut(s) 574, 977
RsaI GTAC 5 cut(s) 561, 582, 814, 829, 840
RsaNI GTAC 5 cut(s) 560, 581, 813, 828, 839
SaqAI TTAA 1 cut(s) 33
SatI GCNGC 3 cut(s) 48, 702, 1168
Sau3AI GATC 3 cut(s) 574, 898, 977
Sau96I GGNCC 2 cut(s) 427, 461
SchI GAGTC 1 cut(s) 392
ScrFI CCNGG 2 cut(s) 627, 956
SduI GDGCHC 4 cut(s) 43, 160, 698, 775
SfaNI GCATC 3 cut(s) 500, 958, 1120
SinI GGWCC 1 cut(s) 461
SnaBI TACGTA 1 cut(s) 180
Sse9I AATT 6 cut(s) 239, 387, 405, 619, 928, 1000
SsiI CCGC 4 cut(s) 86, 642, 654, 749
SspMI CTAG 3 cut(s) 51, 776, 936
StyD4I CCNGG 2 cut(s) 625, 954
StyI CCWWGG 1 cut(s) 1134
TaaI ACNGT 2 cut(s) 38, 693
TaiI ACGT 3 cut(s) 182, 732, 814
TaqI TCGA 5 cut(s) 15, 432, 616, 1050, 1152
TasI AATT 6 cut(s) 239, 387, 405, 619, 928, 1000
TatI WGTACW 2 cut(s) 559, 580
TfiI GAWTC 4 cut(s) 272, 613, 668, 1149
Tru1I TTAA 1 cut(s) 33
Tru9I TTAA 1 cut(s) 33
TscAI CASTG 5 cut(s) 325, 463, 698, 703, 775
TseFI GTSAC 1 cut(s) 342
TseI GCWGC 3 cut(s) 47, 701, 1167
Tsp45I GTSAC 1 cut(s) 342
TspDTI ATGAA 6 cut(s) 82, 260, 660, 938, 1031, 1170
TspGWI ACGGA 1 cut(s) 551
TspRI CASTG 5 cut(s) 325, 463, 698, 703, 775
Van91I CCANNNNNTGG 1 cut(s) 353
VneI GTGCAC 1 cut(s) 694
VpaK11BI GGWCC 1 cut(s) 461
XapI RAATTY 2 cut(s) 239, 619
XceI RCATGY 1 cut(s) 909
XcmI CCANNNNNNNNNTGG 1 cut(s) 404
XspI CTAG 3 cut(s) 51, 776, 936
Zsp2I ATGCAT 1 cut(s) 515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.