pycom15g34510

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008318
Physical Location & Seq
Forward (+)
34312536 .. 34315056
2521 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1002 bp
ATGAAGCTTCACAAAGACTACTATGGCTACTACTACCCCAACCAGTCTCAGAGAGCCGCTTTAGCCGGCCTTTTGGTGGTCCTCTTTCCGGCCTTCCTGCCAAACCTCTTTGGCCCATTGGGCCACGCCTCTCCTTCCATCTTCTCGGAGTGGAATGCCCCGCAACCCCGGCATTCGCGCCTTCTAAGTCCGGCTTTACTTCTTCAATCTTCTGCGGATCAACAGAGCAAGCTTTGGGCTCCGTTGCCCGACCAAAGATGGAAGCATTGTCCTGAAGAGCCTAGAAGTTTATCATCGTTGCCGAAAGAATCTACTGGGTATATTCAGGTGTTCCTTGATGGAGGCTTGAACCAGCAGAAAATGGGGATTTGTGATGCAGTTGCTGTTGCTAAAATTTTGAATGCCACTTTGGTTATTCCACACCTTGAAGTTAATCCTGTGTGGCAAGATTCGAGTTCCTTTGAGGAAATTTTTGATGTGGATCACTTTATTGAAGCCCTACAGGGTGAAGTTTCTATAGTTAAAGTCGTTCCTATTGAATTTTCTTGGAGCACTAGGGAGTATTATGCTACTGGGATACGGATAACTAGAATAAAAACAGCACCTGTTCATGCTTCTTCTGATTGGTATCTGGAAAACGTCCTGCCCATATTGCAGAGATATGGAGTTGCTGCTATCTCCCCATTTTCTCATCGTTTGGCTTTTGAAAACTTGCCTCAAAACATACAGCGCCTACGTTGTAAAGTCAACTTTGAAGCTTTAGCCTTTGTTCCTCATATCCGGGAATTGGGAGAAACCCTTGTTAATCGTCTCCGCTATCCCAATAGAAACCAAAAGGCAGGTACTGGCTCGCAGGACAAAGCAAATGAAATCGAGAAACAGGGAGCTGGGAAGTATGTTGTTTTGCATCTTCGCTTTGATAAAGTATGGCTGCCCATTCAGCCTGTGATTTTGGTGGTGGTAAAGCTGAGAAACTCGCCCTTGCAAAATATCGGCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

334

Amino Acids

37.67

Weight (kDa)

9.11

Isoelectric Point (pI)

63.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 107 - 310 1e-49 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 830
AccII CGCG 1 cut(s) 178
AciI CCGC 4 cut(s) 57, 161, 215, 814
AclWI GGATC 2 cut(s) 225, 489
AcsI RAATTY 3 cut(s) 393, 468, 539
AcuI CTGAAG 1 cut(s) 294
AfaI GTAC 1 cut(s) 844
AfiI CCNNNNNNNGG 3 cut(s) 76, 88, 787
AgsI TTSAA 8 cut(s) 206, 349, 400, 428, 494, 539, 707, 755
AjuI GAANNNNNNNTTGG 2 cut(s) 392, 424
AluBI AGCT 5 cut(s) 7, 232, 758, 887, 967
AluI AGCT 5 cut(s) 7, 232, 758, 887, 967
Alw21I GWGCWC 1 cut(s) 554
Alw26I GTCTC 2 cut(s) 51, 815
AlwI GGATC 2 cut(s) 225, 489
AlwNI CAGNNNCTG 3 cut(s) 383, 605, 845
AoxI GGCC 4 cut(s) 67, 90, 112, 121
ApeKI GCWGC 2 cut(s) 671, 931
ApoI RAATTY 3 cut(s) 393, 468, 539
AspLEI GCGC 2 cut(s) 180, 732
AspS9I GGNCC 3 cut(s) 79, 113, 121
AsuC2I CCSGG 2 cut(s) 169, 782
AsuHPI GGTGA 1 cut(s) 518
AvaII GGWCC 1 cut(s) 79
BanII GRGCYC 1 cut(s) 241
Bbv12I GWGCWC 1 cut(s) 554
BbvI GCAGC 2 cut(s) 658, 918
BccI CCATC 3 cut(s) 146, 252, 332
BciVI GTATCC 1 cut(s) 570
BcnI CCSGG 2 cut(s) 169, 782
BcoDI GTCTC 2 cut(s) 51, 815
BfaI CTAG 3 cut(s) 282, 555, 588
BfmI CTRYAG 2 cut(s) 500, 516
BfoI RGCGCY 1 cut(s) 733
BfuAI ACCTGC 1 cut(s) 830
BfuI GTATCC 1 cut(s) 570
BglI GCCNNNNNGGC 1 cut(s) 120
BisI GCNGC 3 cut(s) 57, 672, 932
BlsI GCNGC 3 cut(s) 58, 673, 933
Bme1390I CCNGG 2 cut(s) 169, 782
Bme18I GGWCC 1 cut(s) 79
BmgT120I GGNCC 3 cut(s) 79, 113, 121
BmiI GGNNCC 1 cut(s) 240
BmrFI CCNGG 2 cut(s) 169, 782
BmrI ACTGGG 2 cut(s) 324, 582
BmsI GCATC 2 cut(s) 364, 916
BmuI ACTGGG 2 cut(s) 324, 582
BpuMI CCSGG 2 cut(s) 169, 782
BsaBI GATNNNNATC 2 cut(s) 480, 627
BsaJI CCNNGG 1 cut(s) 167
Bsc4I CCNNNNNNNGG 3 cut(s) 76, 88, 787
Bse118I RCCGGY 1 cut(s) 65
Bse1I ACTGG 4 cut(s) 43, 319, 577, 850
Bse8I GATNNNNATC 2 cut(s) 480, 627
BseDI CCNNGG 1 cut(s) 167
BseJI GATNNNNATC 2 cut(s) 480, 627
BseLI CCNNNNNNNGG 3 cut(s) 76, 88, 787
BseMII CTCAG 2 cut(s) 62, 959
BseNI ACTGG 4 cut(s) 43, 319, 577, 850
BseXI GCAGC 2 cut(s) 658, 918
BseYI CCCAGC 1 cut(s) 887
Bsh1236I CGCG 1 cut(s) 178
BshFI GGCC 4 cut(s) 69, 92, 114, 123
BsiHKAI GWGCWC 1 cut(s) 554
BsiSI CCGG 5 cut(s) 66, 89, 169, 191, 781
BslI CCNNNNNNNGG 3 cut(s) 76, 88, 787
BsmAI GTCTC 2 cut(s) 51, 815
BsmBI CGTCTC 1 cut(s) 815
BsmI GAATGC 3 cut(s) 160, 172, 406
BsnI GGCC 4 cut(s) 69, 92, 114, 123
Bsp1286I GDGCHC 2 cut(s) 241, 554
Bsp143I GATC 2 cut(s) 217, 481
BspACI CCGC 4 cut(s) 57, 161, 215, 814
BspANI GGCC 4 cut(s) 69, 92, 114, 123
BspCNI CTCAG 2 cut(s) 61, 960
BspFNI CGCG 1 cut(s) 178
BspLI GGNNCC 1 cut(s) 240
BspMI ACCTGC 1 cut(s) 830
BspPI GGATC 2 cut(s) 225, 489
BspQI GCTCTTC 1 cut(s) 270
BsrFI RCCGGY 1 cut(s) 65
BsrI ACTGG 4 cut(s) 43, 319, 577, 850
BssAI RCCGGY 1 cut(s) 65
BssECI CCNNGG 1 cut(s) 167
BssMI GATC 2 cut(s) 217, 481
Bst6I CTCTTC 1 cut(s) 270
BstC8I GCNNGC 3 cut(s) 67, 230, 851
BstDEI CTNAG 3 cut(s) 48, 185, 968
BstFNI CGCG 1 cut(s) 178
BstH2I RGCGCY 1 cut(s) 733
BstHHI GCGC 2 cut(s) 180, 732
BstKTI GATC 2 cut(s) 220, 484
BstMAI GTCTC 2 cut(s) 51, 815
BstMBI GATC 2 cut(s) 217, 481
BstMWI GCNNNNNNNGC 5 cut(s) 62, 120, 169, 652, 940
BstSCI CCNGG 2 cut(s) 167, 780
BstSFI CTRYAG 2 cut(s) 500, 516
BstUI CGCG 1 cut(s) 178
BstV1I GCAGC 2 cut(s) 658, 918
BsuI GTATCC 1 cut(s) 570
BsuRI GGCC 4 cut(s) 69, 92, 114, 123
BveI ACCTGC 1 cut(s) 830
Cac8I GCNNGC 3 cut(s) 67, 230, 851
CaiI CAGNNNCTG 3 cut(s) 383, 605, 845
CfoI GCGC 2 cut(s) 180, 732
Cfr10I RCCGGY 1 cut(s) 65
Cfr13I GGNCC 3 cut(s) 79, 113, 121
Csp6I GTAC 1 cut(s) 843
CviAII CATG 1 cut(s) 611
CviQI GTAC 1 cut(s) 843
DdeI CTNAG 3 cut(s) 48, 185, 968
DpnI GATC 2 cut(s) 219, 483
DpnII GATC 2 cut(s) 217, 481
Eam1104I CTCTTC 1 cut(s) 270
EarI CTCTTC 1 cut(s) 270
Eco24I GRGCYC 1 cut(s) 241
Eco47I GGWCC 1 cut(s) 79
Eco57I CTGAAG 1 cut(s) 294
EcoT38I GRGCYC 1 cut(s) 241
Esp3I CGTCTC 1 cut(s) 815
FaeI CATG 1 cut(s) 614
FalI AAGNNNNNCTT 2 cut(s) 178, 210
FatI CATG 1 cut(s) 610
FauI CCCGC 1 cut(s) 168
Fnu4HI GCNGC 3 cut(s) 57, 672, 932
FriOI GRGCYC 1 cut(s) 241
Fsp4HI GCNGC 3 cut(s) 57, 672, 932
FspBI CTAG 3 cut(s) 282, 555, 588
GlaI GCGC 2 cut(s) 179, 731
GluI GCNGC 3 cut(s) 57, 672, 932
GsaI CCCAGC 1 cut(s) 891
HaeII RGCGCY 1 cut(s) 733
HaeIII GGCC 4 cut(s) 69, 92, 114, 123
HapII CCGG 5 cut(s) 66, 89, 169, 191, 781
HhaI GCGC 2 cut(s) 180, 732
Hin1II CATG 1 cut(s) 614
Hin6I GCGC 2 cut(s) 178, 730
HinP1I GCGC 2 cut(s) 178, 730
HincII GTYRAC 1 cut(s) 748
HindII GTYRAC 1 cut(s) 748
HindIII AAGCTT 3 cut(s) 5, 230, 756
HinfI GANTC 2 cut(s) 308, 449
HpaII CCGG 5 cut(s) 66, 89, 169, 191, 781
HphI GGTGA 1 cut(s) 518
Hpy166II GTNNAC 1 cut(s) 748
Hpy188I TCNGA 3 cut(s) 51, 148, 622
Hpy188III TCNNGA 3 cut(s) 272, 632, 874
Hpy8I GTNNAC 1 cut(s) 748
HpyAV CCTTC 3 cut(s) 103, 144, 191
HpyCH4IV ACGT 2 cut(s) 639, 736
HpyCH4V TGCA 4 cut(s) 377, 655, 907, 985
HpyF10VI GCNNNNNNNGC 5 cut(s) 62, 120, 169, 652, 940
HpyF3I CTNAG 3 cut(s) 48, 185, 968
HpySE526I ACGT 2 cut(s) 639, 736
Hsp92II CATG 1 cut(s) 614
HspAI GCGC 2 cut(s) 178, 730
KroI GCCGGC 1 cut(s) 65
KroNI GCCGGC 1 cut(s) 67
Kzo9I GATC 2 cut(s) 217, 481
LguI GCTCTTC 1 cut(s) 270
LmnI GCTCC 3 cut(s) 244, 549, 884
Lsp1109I GCAGC 2 cut(s) 658, 918
LweI GCATC 2 cut(s) 364, 916
MaeI CTAG 3 cut(s) 282, 555, 588
MaeII ACGT 2 cut(s) 639, 736
MalI GATC 2 cut(s) 219, 483
MboI GATC 2 cut(s) 217, 481
MboII GAAGA 6 cut(s) 133, 194, 201, 287, 609, 902
MhlI GDGCHC 2 cut(s) 241, 554
MluCI AATT 4 cut(s) 393, 468, 539, 785
MnlI CCTC 7 cut(s) 92, 116, 139, 335, 457, 726, 783
MroNI GCCGGC 1 cut(s) 65
MseI TTAA 3 cut(s) 432, 522, 804
MspI CCGG 5 cut(s) 66, 89, 169, 191, 781
MspR9I CCNGG 2 cut(s) 169, 782
Mva1269I GAATGC 3 cut(s) 160, 172, 406
MvnI CGCG 1 cut(s) 178
MwoI GCNNNNNNNGC 5 cut(s) 62, 120, 169, 652, 940
NaeI GCCGGC 1 cut(s) 67
NciI CCSGG 2 cut(s) 169, 782
NdeII GATC 2 cut(s) 217, 481
NgoMIV GCCGGC 1 cut(s) 65
NlaIII CATG 1 cut(s) 614
NlaIV GGNNCC 1 cut(s) 240
PciSI GCTCTTC 1 cut(s) 270
PctI GAATGC 3 cut(s) 160, 172, 406
PdiI GCCGGC 1 cut(s) 67
PfeI GAWTC 2 cut(s) 308, 449
PfoI TCCNGGA 1 cut(s) 780
PkrI GCNGC 3 cut(s) 58, 673, 933
PspFI CCCAGC 1 cut(s) 887
PspN4I GGNNCC 1 cut(s) 240
PspPI GGNCC 3 cut(s) 79, 113, 121
PstNI CAGNNNCTG 3 cut(s) 383, 605, 845
RsaI GTAC 1 cut(s) 844
RsaNI GTAC 1 cut(s) 843
SapI GCTCTTC 1 cut(s) 270
SaqAI TTAA 3 cut(s) 432, 522, 804
SatI GCNGC 3 cut(s) 57, 672, 932
Sau3AI GATC 2 cut(s) 217, 481
Sau96I GGNCC 3 cut(s) 79, 113, 121
ScrFI CCNGG 2 cut(s) 169, 782
SduI GDGCHC 2 cut(s) 241, 554
SfaNI GCATC 2 cut(s) 364, 916
SfcI CTRYAG 2 cut(s) 500, 516
SfiI GGCCNNNNNGGCC 1 cut(s) 120
SinI GGWCC 1 cut(s) 79
Sse9I AATT 4 cut(s) 393, 468, 539, 785
SsiI CCGC 4 cut(s) 57, 161, 215, 814
SspMI CTAG 3 cut(s) 282, 555, 588
StyD4I CCNGG 2 cut(s) 167, 780
TaiI ACGT 2 cut(s) 642, 739
TaqI TCGA 2 cut(s) 452, 873
TasI AATT 4 cut(s) 393, 468, 539, 785
TauI GCSGC 1 cut(s) 59
TfiI GAWTC 2 cut(s) 308, 449
Tru1I TTAA 3 cut(s) 432, 522, 804
Tru9I TTAA 3 cut(s) 432, 522, 804
TseI GCWGC 2 cut(s) 671, 931
TspDTI ATGAA 3 cut(s) 17, 599, 882
TspGWI ACGGA 2 cut(s) 231, 595
VpaK11BI GGWCC 1 cut(s) 79
XapI RAATTY 3 cut(s) 393, 468, 539
XspI CTAG 3 cut(s) 282, 555, 588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.