pycom15g38140

Protein EI24 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008616
Physical Location & Seq
Reverse (-)
38033983 .. 38037819
3837 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 825 bp
ATGATCAAAACAAGAATATACCTTCTCCTCCCGGTTCTCCTAGAACAGAAGGCCCTCCGTCCTCCGTCAGAGTCACAGTCACAGTCACAGCAGAAAACTAATTCATCGGTCTACTTAATTCTGAGAAGAATGGAAGCTACGAAGCCATGGATAAACGCTTTCAGAGCAAAATCAAAGCAAGCCACGCTGCTTTGGCTCGAGGGCTTCCGAGAGGCCTGCTGCCTTCATCGCGTCCTCATCCTCTGCCACCGGTCGAGAAAGCTTATGATACGAACAGGACAGTGTTTTCTATTAAACGGCTTCATTTTCTTAGGAAGTATAATTATCCTTAACTCAGTCATCATCCCAACTCTACACTGGATATTACCTGATAGATGCCCAGAATATAGTTCTCAAGAACTCTGCTCATTTGTTGGTACATTGAAATTTTATTCCGTCTTGCGGCATGGACTCGTACAACTCTTTTATGTAACTTGGTTCTACCCACTGTACGTATTCAGCATTATCCTGAGCAACCTCTGGTACAATGACATTGCTAAGTATGGATTTTCTGCAATGGGGAGATCCACGCCAACCACATTGGATCCTGTCAGACAAAATGATGTTGGTTTGGAAAGACCTGCTGGCGTAGAAGGGCATCTGTTTAATCTGCAGGGTCATGATTGGTATAGGAGAGCAGGTGTATTCAGTACTTCTTTTGACCTGTTTCTTCCTAGAGGTATATGTGACTGGATATATACCATATGTGGGGAAGGCAGTAAATTTTCTGCTTCTGTCCTGGATGTATGCCTATTACTGTTTCGAGTACAAATGGAATTTTTCTGA

Protein Analysis

275

Amino Acids

31.69

Weight (kDa)

9.25

Isoelectric Point (pI)

49.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EI24 PF07264 74 - 182 6.3e-07 Sulfate transporter CysZ/Etoposide-induced protein 2.4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 668
Acc36I ACCTGC 2 cut(s) 628, 668
AccI GTMKAC 1 cut(s) 111
AccII CGCG 1 cut(s) 231
AciI CCGC 1 cut(s) 442
AclWI GGATC 3 cut(s) 558, 578, 591
AcsI RAATTY 3 cut(s) 425, 761, 815
AfaI GTAC 6 cut(s) 418, 456, 491, 524, 691, 807
AfiI CCNNNNNNNGG 2 cut(s) 441, 747
AgeI ACCGGT 1 cut(s) 249
AgsI TTSAA 1 cut(s) 424
AjnI CCWGG 1 cut(s) 777
AluBI AGCT 2 cut(s) 137, 262
AluI AGCT 2 cut(s) 137, 262
AlwI GGATC 3 cut(s) 558, 578, 591
Ama87I CYCGRG 1 cut(s) 197
AoxI GGCC 2 cut(s) 51, 213
ApeKI GCWGC 2 cut(s) 187, 219
ApoI RAATTY 3 cut(s) 425, 761, 815
AsiGI ACCGGT 1 cut(s) 249
AspS9I GGNCC 1 cut(s) 52
AsuC2I CCSGG 1 cut(s) 32
AvaI CYCGRG 1 cut(s) 197
BamHI GGATCC 1 cut(s) 583
BbvI GCAGC 2 cut(s) 174, 206
BceAI ACGGC 1 cut(s) 313
BcgI CGANNNNNNTGC 2 cut(s) 198, 232
BciT130I CCWGG 1 cut(s) 779
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 1 cut(s) 32
BfaI CTAG 2 cut(s) 41, 714
BfmI CTRYAG 1 cut(s) 650
BfuAI ACCTGC 2 cut(s) 628, 668
BisI GCNGC 3 cut(s) 188, 220, 443
BlsI GCNGC 3 cut(s) 189, 221, 444
BmcAI AGTACT 1 cut(s) 691
Bme1390I CCNGG 2 cut(s) 32, 779
BmeT110I CYCGRG 1 cut(s) 197
BmgT120I GGNCC 1 cut(s) 52
BmiI GGNNCC 1 cut(s) 585
BmrFI CCNGG 2 cut(s) 32, 779
BmsI GCATC 2 cut(s) 365, 646
Bpu10I CCTNAGC 1 cut(s) 509
BpuEI CTTGAG 1 cut(s) 378
BpuMI CCSGG 1 cut(s) 32
BsaAI YACGTR 1 cut(s) 493
BsaJI CCNNGG 1 cut(s) 146
BsaWI WCCGGW 1 cut(s) 249
BsaXI ACNNNNNCTCC 2 cut(s) 664, 694
Bsc4I CCNNNNNNNGG 2 cut(s) 441, 747
Bse118I RCCGGY 1 cut(s) 249
Bse1I ACTGG 2 cut(s) 362, 734
Bse3DI GCAATG 2 cut(s) 531, 561
BseBI CCWGG 1 cut(s) 779
BseDI CCNNGG 1 cut(s) 146
BseGI GGATG 3 cut(s) 237, 342, 787
BseLI CCNNNNNNNGG 2 cut(s) 441, 747
BseMI GCAATG 2 cut(s) 531, 561
BseMII CTCAG 3 cut(s) 113, 348, 500
BseNI ACTGG 2 cut(s) 362, 734
BseRI GAGGAG 1 cut(s) 17
BseXI GCAGC 2 cut(s) 174, 206
Bsh1236I CGCG 1 cut(s) 231
Bsh1285I CGRYCG 1 cut(s) 254
BshFI GGCC 2 cut(s) 53, 215
BshTI ACCGGT 1 cut(s) 249
BsiEI CGRYCG 1 cut(s) 254
BsiHKCI CYCGRG 1 cut(s) 197
BsiSI CCGG 2 cut(s) 32, 250
BslI CCNNNNNNNGG 2 cut(s) 441, 747
BsnI GGCC 2 cut(s) 53, 215
BsoBI CYCGRG 1 cut(s) 197
Bsp143I GATC 3 cut(s) 3, 563, 583
Bsp19I CCATGG 1 cut(s) 146
BspACI CCGC 1 cut(s) 442
BspANI GGCC 2 cut(s) 53, 215
BspCNI CTCAG 3 cut(s) 114, 347, 501
BspFNI CGCG 1 cut(s) 231
BspHI TCATGA 1 cut(s) 658
BspLI GGNNCC 1 cut(s) 585
BspMAI CTGCAG 1 cut(s) 654
BspMI ACCTGC 2 cut(s) 628, 668
BspPI GGATC 3 cut(s) 558, 578, 591
BsrDI GCAATG 2 cut(s) 531, 561
BsrFI RCCGGY 1 cut(s) 249
BsrI ACTGG 2 cut(s) 362, 734
BssAI RCCGGY 1 cut(s) 249
BssECI CCNNGG 1 cut(s) 146
BssMI GATC 3 cut(s) 3, 563, 583
BssT1I CCWWGG 1 cut(s) 146
Bst2UI CCWGG 1 cut(s) 779
Bst4CI ACNGT 5 cut(s) 78, 84, 282, 489, 798
BstBAI YACGTR 1 cut(s) 493
BstC8I GCNNGC 3 cut(s) 180, 217, 625
BstDEI CTNAG 5 cut(s) 122, 310, 334, 509, 537
BstDSI CCRYGG 1 cut(s) 146
BstF5I GGATG 3 cut(s) 237, 342, 787
BstFNI CGCG 1 cut(s) 231
BstKTI GATC 3 cut(s) 6, 566, 586
BstMBI GATC 3 cut(s) 3, 563, 583
BstMCI CGRYCG 1 cut(s) 254
BstMWI GCNNNNNNNGC 4 cut(s) 164, 184, 193, 228
BstNI CCWGG 1 cut(s) 779
BstSCI CCNGG 2 cut(s) 30, 777
BstSFI CTRYAG 1 cut(s) 650
BstSNI TACGTA 1 cut(s) 493
BstUI CGCG 1 cut(s) 231
BstV1I GCAGC 2 cut(s) 174, 206
BstX2I RGATCY 2 cut(s) 563, 583
BstYI RGATCY 2 cut(s) 563, 583
BsuRI GGCC 2 cut(s) 53, 215
BtgI CCRYGG 1 cut(s) 146
BtgZI GCGATG 1 cut(s) 212
BtsCI GGATG 3 cut(s) 237, 342, 787
BtsIMutI CAGTG 3 cut(s) 287, 355, 485
BveI ACCTGC 2 cut(s) 628, 668
Cac8I GCNNGC 3 cut(s) 180, 217, 625
CciI TCATGA 1 cut(s) 658
Cfr10I RCCGGY 1 cut(s) 249
Cfr13I GGNCC 1 cut(s) 52
CseI GACGC 1 cut(s) 220
Csp6I GTAC 6 cut(s) 417, 455, 490, 523, 690, 806
CspAI ACCGGT 1 cut(s) 249
CviAII CATG 3 cut(s) 147, 446, 659
CviJI RGCY 9 cut(s) 53, 137, 145, 182, 196, 204, 215, 262, 300
CviKI_1 RGCY 9 cut(s) 53, 137, 145, 182, 196, 204, 215, 262, 300
CviQI GTAC 6 cut(s) 417, 455, 490, 523, 690, 806
DdeI CTNAG 5 cut(s) 122, 310, 334, 509, 537
DpnI GATC 3 cut(s) 5, 565, 585
DpnII GATC 3 cut(s) 3, 563, 583
Eco105I TACGTA 1 cut(s) 493
Eco130I CCWWGG 1 cut(s) 146
Eco147I AGGCCT 1 cut(s) 215
Eco88I CYCGRG 1 cut(s) 197
EcoO109I RGGNCCY 1 cut(s) 52
EcoRII CCWGG 1 cut(s) 777
EcoT14I CCWWGG 1 cut(s) 146
ErhI CCWWGG 1 cut(s) 146
FaeI CATG 3 cut(s) 150, 449, 662
FatI CATG 3 cut(s) 146, 445, 658
FauNDI CATATG 1 cut(s) 743
FbaI TGATCA 1 cut(s) 3
FblI GTMKAC 1 cut(s) 111
Fnu4HI GCNGC 3 cut(s) 188, 220, 443
FokI GGATG 3 cut(s) 224, 329, 794
Fsp4HI GCNGC 3 cut(s) 188, 220, 443
FspBI CTAG 2 cut(s) 41, 714
GluI GCNGC 3 cut(s) 188, 220, 443
HaeIII GGCC 2 cut(s) 53, 215
HapII CCGG 2 cut(s) 32, 250
HgaI GACGC 1 cut(s) 220
Hin1II CATG 3 cut(s) 150, 449, 662
HindIII AAGCTT 1 cut(s) 260
HinfI GANTC 2 cut(s) 71, 450
HpaII CCGG 2 cut(s) 32, 250
Hpy166II GTNNAC 1 cut(s) 112
Hpy188I TCNGA 6 cut(s) 70, 123, 164, 209, 593, 824
Hpy188III TCNNGA 4 cut(s) 255, 395, 508, 659
Hpy8I GTNNAC 1 cut(s) 112
HpyAV CCTTC 5 cut(s) 32, 43, 233, 626, 746
HpyCH4III ACNGT 5 cut(s) 78, 84, 282, 489, 798
HpyCH4IV ACGT 1 cut(s) 492
HpyCH4V TGCA 2 cut(s) 554, 652
HpyF10VI GCNNNNNNNGC 4 cut(s) 164, 184, 193, 228
HpyF3I CTNAG 5 cut(s) 122, 310, 334, 509, 537
HpySE526I ACGT 1 cut(s) 492
Hsp92II CATG 3 cut(s) 150, 449, 662
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 3 cut(s) 3, 563, 583
Lsp1109I GCAGC 2 cut(s) 174, 206
LweI GCATC 2 cut(s) 365, 646
MaeI CTAG 2 cut(s) 41, 714
MaeII ACGT 1 cut(s) 492
MaeIII GTNAC 5 cut(s) 72, 78, 84, 469, 725
MalI GATC 3 cut(s) 5, 565, 585
MboI GATC 3 cut(s) 3, 563, 583
MboII GAAGA 2 cut(s) 138, 701
MflI RGATCY 2 cut(s) 563, 583
MluCI AATT 6 cut(s) 100, 117, 321, 425, 761, 815
MlyI GAGTC 2 cut(s) 80, 444
MnlI CCTC 9 cut(s) 38, 65, 72, 193, 205, 245, 251, 527, 710
MseI TTAA 4 cut(s) 116, 293, 330, 645
MspI CCGG 2 cut(s) 32, 250
MspR9I CCNGG 2 cut(s) 32, 779
MvaI CCWGG 1 cut(s) 779
MvnI CGCG 1 cut(s) 231
MwoI GCNNNNNNNGC 4 cut(s) 164, 184, 193, 228
NciI CCSGG 1 cut(s) 32
NcoI CCATGG 1 cut(s) 146
NdeI CATATG 1 cut(s) 743
NdeII GATC 3 cut(s) 3, 563, 583
NlaIII CATG 3 cut(s) 150, 449, 662
NlaIV GGNNCC 1 cut(s) 585
NmuCI GTSAC 4 cut(s) 72, 78, 84, 725
PaeR7I CTCGAG 1 cut(s) 197
PagI TCATGA 1 cut(s) 658
PaqCI CACCTGC 1 cut(s) 668
PceI AGGCCT 1 cut(s) 215
PfoI TCCNGGA 1 cut(s) 777
PinAI ACCGGT 1 cut(s) 249
PkrI GCNGC 3 cut(s) 189, 221, 444
PleI GAGTC 2 cut(s) 79, 444
PpsI GAGTC 2 cut(s) 79, 444
Ppu21I YACGTR 1 cut(s) 493
Psp6I CCWGG 1 cut(s) 777
PspGI CCWGG 1 cut(s) 777
PspN4I GGNNCC 1 cut(s) 585
PspPI GGNCC 1 cut(s) 52
PspXI VCTCGAGB 1 cut(s) 197
PstI CTGCAG 1 cut(s) 654
PsuI RGATCY 2 cut(s) 563, 583
RsaI GTAC 6 cut(s) 418, 456, 491, 524, 691, 807
RsaNI GTAC 6 cut(s) 417, 455, 490, 523, 690, 806
SaqAI TTAA 4 cut(s) 116, 293, 330, 645
SatI GCNGC 3 cut(s) 188, 220, 443
Sau3AI GATC 3 cut(s) 3, 563, 583
Sau96I GGNCC 1 cut(s) 52
ScaI AGTACT 1 cut(s) 691
SchI GAGTC 2 cut(s) 80, 444
ScrFI CCNGG 2 cut(s) 32, 779
SfaNI GCATC 2 cut(s) 365, 646
SfcI CTRYAG 1 cut(s) 650
Sfr274I CTCGAG 1 cut(s) 197
SlaI CTCGAG 1 cut(s) 197
SmlI CTYRAG 2 cut(s) 197, 393
SmoI CTYRAG 2 cut(s) 197, 393
SnaBI TACGTA 1 cut(s) 493
Sse9I AATT 6 cut(s) 100, 117, 321, 425, 761, 815
SseBI AGGCCT 1 cut(s) 215
SsiI CCGC 1 cut(s) 442
SspMI CTAG 2 cut(s) 41, 714
StuI AGGCCT 1 cut(s) 215
StyD4I CCNGG 2 cut(s) 30, 777
StyI CCWWGG 1 cut(s) 146
TaaI ACNGT 5 cut(s) 78, 84, 282, 489, 798
TaiI ACGT 1 cut(s) 495
TaqI TCGA 3 cut(s) 198, 254, 802
TaqII GACCGA 1 cut(s) 97
TasI AATT 6 cut(s) 100, 117, 321, 425, 761, 815
TatI WGTACW 2 cut(s) 689, 805
TauI GCSGC 1 cut(s) 445
Tru1I TTAA 4 cut(s) 116, 293, 330, 645
Tru9I TTAA 4 cut(s) 116, 293, 330, 645
TscAI CASTG 3 cut(s) 287, 362, 492
TseFI GTSAC 4 cut(s) 72, 78, 84, 725
TseI GCWGC 2 cut(s) 187, 219
Tsp45I GTSAC 4 cut(s) 72, 78, 84, 725
TspDTI ATGAA 3 cut(s) 93, 215, 292
TspGWI ACGGA 3 cut(s) 47, 54, 424
TspRI CASTG 3 cut(s) 287, 362, 492
XapI RAATTY 3 cut(s) 425, 761, 815
XcmI CCANNNNNNNNNTGG 1 cut(s) 354
XhoI CTCGAG 1 cut(s) 197
XmiI GTMKAC 1 cut(s) 111
XspI CTAG 2 cut(s) 41, 714
ZrmI AGTACT 1 cut(s) 691
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.