pycom16g01260

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0003989
Physical Location & Seq
Forward (+)
806690 .. 808267
1578 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1026 bp
ATGGTTAGCAGCTCAGCCATGGCAAAACCCCCATTTGCTTCACGCCTTCATCTGGTGTTGGTGTTCTGCATTGCTATTATTTCTTCTCTAGCTGTTTCCACTTCTTTTGCAGAGCTTCAGAGATTGGAACACGCAACAAAAAGAGATGATGGGTCGCTTAGCTTTTTGGTGGTTGGAGATTGGGGCAGAAGAGGGTTATATAACCAATCCAAAGTTGCTCTACAGATGGGAAGGATTGGAGAGCAATTAGATATAGATTTTGTCGTATCCACTGGGGATAATTTTTACGGCGATGGATTGAAAAATGTAAATGATCCAGCATTTTTAGAGTCCTTTAAAAATATTTACACAGCTAAGAGCTTGCAAAAACAGTGGTATAGTGTTTTAGGAAATCATGATTATAGGGGTAATACTGAGGCACAATTAAGCCCTGCCCTCCGAAAGATCGATAGTCGATGGTTTTGCCAGAGATCCTTTATTGTTAATGCAGGAATTGCGGAATTATTTTTTGTGGACTCCACTCCATTTGTGAACAAATACTTGGTCGACAAAGACCACAATTATGATTGGCGAGGCGTGTCTCCGCGGGCAACTTACCTCGCTGACCTTCTACAGGATTTGAAACAAGCATTGAATAAATCAAGTGCAAAGTGGAAGATAGTGGTTGGTCACCATGCAATCAGAAGTGCTGGGCATCATGGCGACACTCCGGAGCTCATAAGTCTCCTTTTACCAATGCTTAAGGTTTATAATGTTGATATGTACATGAATGGACACGATCATTGTTTGGAGCACATTACTAGCCTTGATAACCCGATTCAGTATTTAACAAGTGGGGCAGGTTCAAAAGCATGGAGGGGTGATATTAAGAGATACAACAATCGAGCCATGAAATTCTACTATGATGGTCAAGGTTTCATGTCCGTGAAGCTGGCTGGATCGGATGCTGAAATTGCATTCTATGATGTTTCAGGCAGGGTTTTGTATACATGGAAGGCTTCTAAGCAGCTTCACTCGACCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00740 ko01100 ko04142 ko04380 ko05323 map00740 map01100 map04142 map04380 map05323
Pfam Domains
Protein Families
Metallophos

Protein Analysis

342

Amino Acids

38.4

Weight (kDa)

8.9

Isoelectric Point (pI)

39.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 54 - 262 1.1e-19 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 750
Acc36I ACCTGC 1 cut(s) 830
AccI GTMKAC 2 cut(s) 546, 986
AccII CGCG 1 cut(s) 586
AccIII TCCGGA 1 cut(s) 709
AciI CCGC 3 cut(s) 497, 584, 586
AclWI GGATC 3 cut(s) 308, 465, 946
AcsI RAATTY 1 cut(s) 893
AcuI CTGAAG 1 cut(s) 101
AfaI GTAC 1 cut(s) 764
AfiI CCNNNNNNNGG 2 cut(s) 52, 613
AflII CTTAAG 1 cut(s) 740
AgsI TTSAA 4 cut(s) 301, 622, 634, 846
AjuI GAANNNNNNNTTGG 2 cut(s) 524, 556
AluBI AGCT 9 cut(s) 12, 92, 115, 162, 353, 360, 715, 931, 1009
AluI AGCT 9 cut(s) 12, 92, 115, 162, 353, 360, 715, 931, 1009
Alw21I GWGCWC 2 cut(s) 717, 795
Alw26I GTCTC 2 cut(s) 585, 728
AlwI GGATC 3 cut(s) 308, 465, 946
Aor13HI TCCGGA 1 cut(s) 709
ApeKI GCWGC 2 cut(s) 9, 1006
ApoI RAATTY 1 cut(s) 893
ArsI GACNNNNNNTTYG 2 cut(s) 528, 560
AsuHPI GGTGA 2 cut(s) 662, 872
BanII GRGCYC 1 cut(s) 717
Bbv12I GWGCWC 2 cut(s) 717, 795
BbvI GCAGC 2 cut(s) 21, 1018
BccI CCATC 5 cut(s) 143, 220, 287, 450, 899
BceAI ACGGC 1 cut(s) 304
BcgI CGANNNNNNTGC 2 cut(s) 444, 478
BciVI GTATCC 1 cut(s) 277
BcoDI GTCTC 2 cut(s) 585, 728
BfaI CTAG 2 cut(s) 89, 801
BfmI CTRYAG 2 cut(s) 221, 611
BfrI CTTAAG 1 cut(s) 740
BfuAI ACCTGC 1 cut(s) 830
BfuI GTATCC 1 cut(s) 277
BisI GCNGC 2 cut(s) 10, 1007
BlpI GCTNAGC 2 cut(s) 13, 158
BlsI GCNGC 2 cut(s) 11, 1008
BmrI ACTGGG 1 cut(s) 282
BmsI GCATC 2 cut(s) 703, 934
BmuI ACTGGG 1 cut(s) 282
Bpu1102I GCTNAGC 2 cut(s) 13, 158
Bsa29I ATCGAT 1 cut(s) 447
BsaJI CCNNGG 2 cut(s) 18, 584
BsaWI WCCGGW 1 cut(s) 709
Bsc4I CCNNNNNNNGG 2 cut(s) 52, 613
Bse1I ACTGG 1 cut(s) 277
Bse3DI GCAATG 1 cut(s) 69
BseAI TCCGGA 1 cut(s) 709
BseCI ATCGAT 1 cut(s) 447
BseDI CCNNGG 2 cut(s) 18, 584
BseGI GGATG 1 cut(s) 949
BseLI CCNNNNNNNGG 2 cut(s) 52, 613
BseMI GCAATG 1 cut(s) 69
BseMII CTCAG 2 cut(s) 27, 405
BseNI ACTGG 1 cut(s) 277
BseXI GCAGC 2 cut(s) 21, 1018
BseYI CCCAGC 1 cut(s) 689
Bsh1236I CGCG 1 cut(s) 586
BshVI ATCGAT 1 cut(s) 447
BsiHKAI GWGCWC 2 cut(s) 717, 795
BsiSI CCGG 1 cut(s) 710
BslI CCNNNNNNNGG 2 cut(s) 52, 613
BsmAI GTCTC 2 cut(s) 585, 728
BsmI GAATGC 1 cut(s) 956
Bsp1286I GDGCHC 2 cut(s) 717, 795
Bsp13I TCCGGA 1 cut(s) 709
Bsp1407I TGTACA 1 cut(s) 762
Bsp143I GATC 5 cut(s) 313, 444, 470, 778, 938
Bsp1720I GCTNAGC 2 cut(s) 13, 158
Bsp19I CCATGG 1 cut(s) 18
BspACI CCGC 3 cut(s) 497, 584, 586
BspCNI CTCAG 2 cut(s) 26, 406
BspDI ATCGAT 1 cut(s) 447
BspEI TCCGGA 1 cut(s) 709
BspFNI CGCG 1 cut(s) 586
BspHI TCATGA 1 cut(s) 394
BspMI ACCTGC 1 cut(s) 830
BspPI GGATC 3 cut(s) 308, 465, 946
BspTI CTTAAG 1 cut(s) 740
BsrDI GCAATG 1 cut(s) 69
BsrGI TGTACA 1 cut(s) 762
BsrI ACTGG 1 cut(s) 277
BssECI CCNNGG 2 cut(s) 18, 584
BssMI GATC 5 cut(s) 313, 444, 470, 778, 938
BssNAI GTATAC 1 cut(s) 987
BssT1I CCWWGG 1 cut(s) 18
Bst1107I GTATAC 1 cut(s) 987
Bst4CI ACNGT 1 cut(s) 372
Bst6I CTCTTC 1 cut(s) 184
BstAFI CTTAAG 1 cut(s) 740
BstAPI GCANNNNNTGC 1 cut(s) 494
BstAUI TGTACA 1 cut(s) 762
BstC8I GCNNGC 3 cut(s) 362, 588, 933
BstDEI CTNAG 5 cut(s) 13, 158, 354, 414, 1002
BstDSI CCRYGG 2 cut(s) 18, 584
BstEII GGTNACC 1 cut(s) 668
BstENI CCTNNNNNAGG 1 cut(s) 611
BstF5I GGATG 1 cut(s) 949
BstFNI CGCG 1 cut(s) 586
BstKTI GATC 5 cut(s) 316, 447, 473, 781, 941
BstMAI GTCTC 2 cut(s) 585, 728
BstMBI GATC 5 cut(s) 313, 444, 470, 778, 938
BstMWI GCNNNNNNNGC 2 cut(s) 494, 953
BstPI GGTNACC 1 cut(s) 668
BstSFI CTRYAG 2 cut(s) 221, 611
BstUI CGCG 1 cut(s) 586
BstV1I GCAGC 2 cut(s) 21, 1018
BstX2I RGATCY 1 cut(s) 470
BstYI RGATCY 1 cut(s) 470
BstZ17I GTATAC 1 cut(s) 987
Bsu15I ATCGAT 1 cut(s) 447
BsuI GTATCC 1 cut(s) 277
BsuTUI ATCGAT 1 cut(s) 447
BtgI CCRYGG 2 cut(s) 18, 584
BtgZI GCGATG 1 cut(s) 306
BtsCI GGATG 1 cut(s) 949
BtsIMutI CAGTG 2 cut(s) 270, 377
BveI ACCTGC 1 cut(s) 830
Cac8I GCNNGC 3 cut(s) 362, 588, 933
CciI TCATGA 1 cut(s) 394
Cfr42I CCGCGG 1 cut(s) 587
ClaI ATCGAT 1 cut(s) 447
Csp6I GTAC 1 cut(s) 763
CspCI CAANNNNNGTGG 6 cut(s) 88, 123, 353, 388, 508, 543
CviQI GTAC 1 cut(s) 763
DdeI CTNAG 5 cut(s) 13, 158, 354, 414, 1002
DpnI GATC 5 cut(s) 315, 446, 472, 780, 940
DpnII GATC 5 cut(s) 313, 444, 470, 778, 938
DraI TTTAAA 1 cut(s) 337
Eam1104I CTCTTC 1 cut(s) 184
EarI CTCTTC 1 cut(s) 184
Ecl136II GAGCTC 1 cut(s) 715
Eco130I CCWWGG 1 cut(s) 18
Eco24I GRGCYC 1 cut(s) 717
Eco53kI GAGCTC 1 cut(s) 715
Eco57I CTGAAG 1 cut(s) 101
Eco91I GGTNACC 1 cut(s) 668
EcoICRI GAGCTC 1 cut(s) 715
EcoNI CCTNNNNNAGG 1 cut(s) 611
EcoO65I GGTNACC 1 cut(s) 668
EcoT14I CCWWGG 1 cut(s) 18
EcoT38I GRGCYC 1 cut(s) 717
ErhI CCWWGG 1 cut(s) 18
FauI CCCGC 1 cut(s) 579
FblI GTMKAC 2 cut(s) 546, 986
Fnu4HI GCNGC 2 cut(s) 10, 1007
FokI GGATG 1 cut(s) 956
FriOI GRGCYC 1 cut(s) 717
Fsp4HI GCNGC 2 cut(s) 10, 1007
FspBI CTAG 2 cut(s) 89, 801
GluI GCNGC 2 cut(s) 10, 1007
GsaI CCCAGC 1 cut(s) 693
HapII CCGG 1 cut(s) 710
HincII GTYRAC 1 cut(s) 547
HindII GTYRAC 1 cut(s) 547
HinfI GANTC 3 cut(s) 329, 515, 817
HpaII CCGG 1 cut(s) 710
HphI GGTGA 2 cut(s) 662, 872
Hpy166II GTNNAC 4 cut(s) 514, 532, 547, 987
Hpy188I TCNGA 4 cut(s) 120, 440, 683, 943
Hpy188III TCNNGA 2 cut(s) 395, 710
Hpy8I GTNNAC 4 cut(s) 514, 532, 547, 987
HpyAV CCTTC 4 cut(s) 56, 225, 617, 988
HpyCH4III ACNGT 1 cut(s) 372
HpyCH4V TGCA 7 cut(s) 69, 110, 364, 488, 647, 677, 956
HpyF10VI GCNNNNNNNGC 2 cut(s) 494, 953
HpyF3I CTNAG 5 cut(s) 13, 158, 354, 414, 1002
Kpn2I TCCGGA 1 cut(s) 709
KspI CCGCGG 1 cut(s) 587
Kzo9I GATC 5 cut(s) 313, 444, 470, 778, 938
LmnI GCTCC 2 cut(s) 712, 790
Lsp1109I GCAGC 2 cut(s) 21, 1018
LweI GCATC 2 cut(s) 703, 934
MaeI CTAG 2 cut(s) 89, 801
MaeIII GTNAC 1 cut(s) 668
MalI GATC 5 cut(s) 315, 446, 472, 780, 940
MboI GATC 5 cut(s) 313, 444, 470, 778, 938
MboII GAAGA 3 cut(s) 75, 201, 667
MflI RGATCY 1 cut(s) 470
MhlI GDGCHC 2 cut(s) 717, 795
MluCI AATT 8 cut(s) 245, 280, 422, 492, 500, 559, 893, 951
MlyI GAGTC 2 cut(s) 338, 509
MmeI TCCRAC 1 cut(s) 154
MnlI CCTC 6 cut(s) 185, 409, 446, 566, 608, 849
MroI TCCGGA 1 cut(s) 709
MseI TTAA 6 cut(s) 336, 425, 483, 741, 827, 867
MslI CAYNNNNRTG 2 cut(s) 561, 923
MspA1I CMGCKG 1 cut(s) 586
MspCI CTTAAG 1 cut(s) 740
MspI CCGG 1 cut(s) 710
Mva1269I GAATGC 1 cut(s) 956
MvnI CGCG 1 cut(s) 586
MwoI GCNNNNNNNGC 2 cut(s) 494, 953
NcoI CCATGG 1 cut(s) 18
NdeII GATC 5 cut(s) 313, 444, 470, 778, 938
NmuCI GTSAC 1 cut(s) 668
PagI TCATGA 1 cut(s) 394
PctI GAATGC 1 cut(s) 956
PfeI GAWTC 1 cut(s) 817
PkrI GCNGC 2 cut(s) 11, 1008
PleI GAGTC 2 cut(s) 337, 509
PpsI GAGTC 2 cut(s) 337, 509
PsiI TTATAA 1 cut(s) 750
Psp124BI GAGCTC 1 cut(s) 717
PspEI GGTNACC 1 cut(s) 668
PspFI CCCAGC 1 cut(s) 689
PsuI RGATCY 1 cut(s) 470
RsaI GTAC 1 cut(s) 764
RsaNI GTAC 1 cut(s) 763
RseI CAYNNNNRTG 2 cut(s) 561, 923
SacI GAGCTC 1 cut(s) 717
SacII CCGCGG 1 cut(s) 587
SalI GTCGAC 1 cut(s) 545
SaqAI TTAA 6 cut(s) 336, 425, 483, 741, 827, 867
SatI GCNGC 2 cut(s) 10, 1007
Sau3AI GATC 5 cut(s) 313, 444, 470, 778, 938
SchI GAGTC 2 cut(s) 338, 509
SduI GDGCHC 2 cut(s) 717, 795
SfaNI GCATC 2 cut(s) 703, 934
SfcI CTRYAG 2 cut(s) 221, 611
Sfr303I CCGCGG 1 cut(s) 587
SgrBI CCGCGG 1 cut(s) 587
SmiMI CAYNNNNRTG 2 cut(s) 561, 923
SmlI CTYRAG 1 cut(s) 740
SmoI CTYRAG 1 cut(s) 740
Sse9I AATT 8 cut(s) 245, 280, 422, 492, 500, 559, 893, 951
SsiI CCGC 3 cut(s) 497, 584, 586
SspI AATATT 1 cut(s) 343
SspMI CTAG 2 cut(s) 89, 801
SstI GAGCTC 1 cut(s) 717
StyI CCWWGG 1 cut(s) 18
TaaI ACNGT 1 cut(s) 372
TaqI TCGA 5 cut(s) 447, 454, 546, 883, 1016
TasI AATT 8 cut(s) 245, 280, 422, 492, 500, 559, 893, 951
TatI WGTACW 1 cut(s) 762
TfiI GAWTC 1 cut(s) 817
Tru1I TTAA 6 cut(s) 336, 425, 483, 741, 827, 867
Tru9I TTAA 6 cut(s) 336, 425, 483, 741, 827, 867
TscAI CASTG 2 cut(s) 277, 377
TseFI GTSAC 1 cut(s) 668
TseI GCWGC 2 cut(s) 9, 1006
Tsp45I GTSAC 1 cut(s) 668
TspDTI ATGAA 4 cut(s) 38, 782, 905, 907
TspGWI ACGGA 1 cut(s) 913
TspRI CASTG 2 cut(s) 277, 377
Vha464I CTTAAG 1 cut(s) 740
XagI CCTNNNNNAGG 1 cut(s) 611
XapI RAATTY 1 cut(s) 893
XmiI GTMKAC 2 cut(s) 546, 986
XspI CTAG 2 cut(s) 89, 801
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.