pycom16g03170

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0011786
Physical Location & Seq
Forward (+)
2006545 .. 2008831
2287 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2142 bp
ATGGACCCACCACCAGTGGCCCCGCCGCTGCAACCCCACCACCTCAACTACCCGGAGTCCCTCGACTCCTCCCCTCGCTCCCGCAACACCGACTCATGGGACGATCCCATCCCCTCCACCAACAGGCTCCGCCTCATGTGCAGCTACGGCGGCCACATCGTCCCCCGCCCCCACGACAAGTCGCTATGCTACGTCGGTGGCGACACCCGAATCGTCGTCGTCGACCGCCAGACCTCCCTCTCCGAACTCTCCAACCGCCTCTCCAAGACTCTACTCAACGGCCGACCCTTCACCCTCAAGTACCAGCTCCCCAGCGAAGACCTCGATTCCTTAATCTCCGTCACCACCGACGAGGACCTCGACAACATGATCGACGAGTACGAACGCACGGCTAACCACAGCGGCGCTTCGTCCAAACCCTCACGCCTCCGTCTCTTTCTCTTCCCCCTGAAGCCCGAGTCGTCGCAATCGATGGGGCCCATTCTCGATATTTCAGCCAATAAGTCTGAGGACTGGTTCTTGAACGCGCTCAATGGCGAGTCCGATATGCTCAATCGGGGCTTCTCCGACTCCGCCTCCGTCAATTGCTTGCTGGGTCTCGACGACGATTCGATTTCAGGCGGGGCTGCCAATAATTTGGACTCCGGTTCGAGAGACGTCGAGGCTTCAGTTCCTGCGAATTGCAAGAATGCCAAACAGGGCGGAGGTGGCGGCGGTGGGAACGGGCAGGACGTTCACTCCGTCCCCGATTCGCCGATGCTTGAGAACTCCTCTTCGTTTGGGTCCACGTCATCGTCGCCATCGCTTGCGAACTTGCCGCCGATTCGGGTCCACGTGGAAGATGGTGGCGGCGGAGGAGGTGGAGATCGGATTAGAGTGCAGGATCATAAGATGGGGATAGAAGAGCAGTTCGCTCGGATGACTGTTGGTGGTGGTGTTGGCCCTGTTGGGCAGAGGCAAGACGACGGTGGCTTCGCGGTCCTTTCATCGCCTCCTCCGATTCCCACTACCATCGTGGCATCCGCCGCGCCAATGAGCACGGCCACCGATTACTTGAATCGAGTAGTTTCGGACGACGAGCGATCCGATCACGGCGCCCCAGTTGGGTATCGAAAACCGACGCCTCCTCAGCCTCAGCTTCAGCCACAGACTCTGCCTCCTCAGTCTCAGCAGCCTAAATCTAGTGGCGTTGTTGATTTGCCCTCCCCAGATTCTGTTTCAAGTGACAATAGTTTATCAAATGCCATGTCTCGCCCAAAACCTGTAATTTATCAAGACCCAGTGGTTCAAATCCCCTCTGCAAACGCCAGGTTTCCGGCAAACCTAGTTGATCCGAAATTAAATCTTTCCGATCCAAACACTCGGATTCAGATGCAACAACAGGTTCACGATTCTGGGTACGTTTTGCAATCGCAATTCGATCATCAACAGCAGCAGCAGCAGCAGCAACACCAACAACACCCACAACAAGCACAACAGCCACATCAATTTATACACGCTGGAACACATTACATCCAGCAACACCCAGGTTCGGTCCCCATTCCGGCGTACTACCCAGTATATCCGCCCCAGCAGCAACAGCACCACCATCATCATCCCCAGCTTGATCAGCAGCAGCAGCAGCAATACCAAGTTTATTACATGCCTACCAGGCAGCCACAACCTTACACCACTTTGCCAGTTCAGCAATCGAATATCAGCGATTCTGCCGCTCCTATCCCTTCGACCCGCTCCCAAACGCCTCCTAACCCTGCCATGTCCTCCCCTTCTGCGCCTTACAACCAGATAAGGAATCCCCAAATTGCCAAACCCGAACTGGGTGCCCCAACAGGGGTGTACAGAACGGCAACCACAGCAGCTCCGCCTCTAGTTCAAGTCCCATCTGCTCAGCATCAGCAGCAATTTGTCGGCTACCCCACCCAGATGCACCATCCGTCCCAATCGGCCGTTCCTTCTTCTGGGGGCAATGCCAGTTATGCCTATGAATATGCTACTGATCCCTCTCATGCGCAGATATACTACACACAGCCTCTGGCGCCCTCAATGCCTTCTCAGTACCAAACCATGACGTCCGCGGCCGCCATGGGGCTGCCGGAAGTCTCTGCTCAGCTTCCCACCGACAGCATCAAGCAGCAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

714

Amino Acids

77.28

Weight (kDa)

5.7

Isoelectric Point (pI)

69.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 58 - 149 3e-17 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011786)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G01190
fragaria_vesca FvH4_4g26650 FvH4_4g26650 FvH4_4g26650
malus_domestica MD13G1034900.v1.1 MD16G1036800.v1.1
prunus_persica Prupe.1G317300_v2.0.a1
pyrus_communis pycom13g03050 pycom16g03170
rosa_chinensis RchiOBHm_Chr4g0434361
rosa_laevigata RLG00000006680
rosa_multiflora Rmu_sc0016328.1_g000003
rosa_roxburghii Rroxscaffold_5G00375720
rosa_rugosa Rorug04G0276300
rosa_samantha Rh4AG331400 Rh4BG339300 Rh4CG354400 Rh4DG334400
rosa_wichuraiana Rw4G028740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 660, 2072
Acc16I TGCGCA 1 cut(s) 2010
AccB1I GGYRCC 3 cut(s) 1094, 1820, 2035
AccBSI CCGCTC 2 cut(s) 1712, 1731
AccI GTMKAC 1 cut(s) 222
AccII CGCG 4 cut(s) 527, 977, 1028, 2075
AclWI GGATC 6 cut(s) 98, 891, 1077, 1325, 1346, 1991
AcoI YGGCCR 5 cut(s) 151, 280, 1041, 1944, 2076
AcuI CTGAAG 3 cut(s) 470, 651, 1124
AcvI CACGTG 1 cut(s) 835
AcyI GRCGYC 5 cut(s) 657, 1095, 1121, 2036, 2069
AfaI GTAC 6 cut(s) 302, 380, 1400, 1550, 1838, 2057
AgsI TTSAA 5 cut(s) 523, 1057, 1221, 1289, 1874
AjiI CACGTC 1 cut(s) 789
AjnI CCWGG 3 cut(s) 1307, 1525, 1649
AjuI GAANNNNNNNTTGG 2 cut(s) 1932, 1964
AluBI AGCT 6 cut(s) 144, 307, 1138, 1603, 1859, 2110
AluI AGCT 6 cut(s) 144, 307, 1138, 1603, 1859, 2110
Alw21I GWGCWC 1 cut(s) 1040
Alw26I GTCTC 6 cut(s) 437, 602, 648, 1170, 1254, 2104
AlwI GGATC 6 cut(s) 98, 891, 1077, 1325, 1346, 1991
AlwNI CAGNNNCTG 4 cut(s) 674, 1153, 1214, 2032
Ama87I CYCGRG 1 cut(s) 455
AoxI GGCC 8 cut(s) 18, 151, 280, 476, 940, 1041, 1944, 2076
ApaI GGGCCC 1 cut(s) 480
ArsI GACNNNNNNTTYG 4 cut(s) 956, 988, 1232, 1264
AspLEI GCGC 7 cut(s) 407, 529, 1030, 1097, 1774, 2011, 2038
AsuC2I CCSGG 1 cut(s) 53
AsuHPI GGTGA 2 cut(s) 283, 334
AvaI CYCGRG 1 cut(s) 455
AvaII GGWCC 6 cut(s) 4, 355, 783, 829, 979, 1534
BaeGI GKGCMC 2 cut(s) 480, 1825
BanI GGYRCC 3 cut(s) 1094, 1820, 2035
BanII GRGCYC 1 cut(s) 480
BbrPI CACGTG 1 cut(s) 835
BbsI GAAGAC 1 cut(s) 324
Bbv12I GWGCWC 1 cut(s) 1040
BbvCI CCTCAGC 2 cut(s) 1128, 1134
BccI CCATC 9 cut(s) 116, 466, 808, 836, 886, 1019, 1596, 1888, 1938
BceAI ACGGC 7 cut(s) 163, 295, 405, 1056, 1108, 1860, 1931
BcgI CGANNNNNNTGC 4 cut(s) 896, 930, 1802, 1836
BciT130I CCWGG 3 cut(s) 1309, 1527, 1651
BclI TGATCA 1 cut(s) 1606
BcnI CCSGG 1 cut(s) 53
BcoDI GTCTC 6 cut(s) 437, 602, 648, 1170, 1254, 2104
BfaI CTAG 3 cut(s) 1182, 1325, 1868
BfoI RGCGCY 3 cut(s) 408, 1098, 2039
BglI GCCNNNNNGGC 1 cut(s) 1651
BlpI GCTNAGC 2 cut(s) 1887, 2106
Bme1390I CCNGG 4 cut(s) 53, 1309, 1527, 1651
Bme18I GGWCC 6 cut(s) 4, 355, 783, 829, 979, 1534
BmeT110I CYCGRG 1 cut(s) 455
BmgBI CACGTC 1 cut(s) 789
BmrFI CCNGG 4 cut(s) 53, 1309, 1527, 1651
BmrI ACTGGG 4 cut(s) 1094, 1274, 1550, 1826
BmsI GCATC 6 cut(s) 747, 1028, 1362, 1900, 1914, 2133
BmuI ACTGGG 4 cut(s) 1094, 1274, 1550, 1826
BpiI GAAGAC 1 cut(s) 324
BplI GAGNNNNNCTC 2 cut(s) 755, 787
Bpu10I CCTNAGC 2 cut(s) 1128, 1134
Bpu1102I GCTNAGC 2 cut(s) 1887, 2106
BpuEI CTTGAG 2 cut(s) 281, 782
BpuMI CCSGG 1 cut(s) 53
Bsa29I ATCGAT 1 cut(s) 470
BsaAI YACGTR 1 cut(s) 835
BsaHI GRCGYC 5 cut(s) 657, 1095, 1121, 2036, 2069
BsaI GGTCTC 1 cut(s) 602
BsaJI CCNNGG 3 cut(s) 1525, 2073, 2082
BsaWI WCCGGW 1 cut(s) 644
Bse1I ACTGG 8 cut(s) 14, 518, 1100, 1280, 1556, 1679, 1821, 1971
Bse3DI GCAATG 1 cut(s) 1972
BseBI CCWGG 3 cut(s) 1309, 1527, 1651
BseCI ATCGAT 1 cut(s) 470
BseDI CCNNGG 3 cut(s) 1525, 2073, 2082
BseGI GGATG 6 cut(s) 108, 924, 1019, 1512, 1594, 1930
BseMI GCAATG 1 cut(s) 1972
BseMII CTCAG 8 cut(s) 498, 1142, 1148, 1175, 1181, 1901, 2066, 2120
BseNI ACTGG 8 cut(s) 14, 518, 1100, 1280, 1556, 1679, 1821, 1971
BseRI GAGGAG 6 cut(s) 58, 760, 870, 984, 1116, 1149
BseSI GKGCMC 2 cut(s) 480, 1825
BseX3I CGGCCG 3 cut(s) 280, 1944, 2076
BseYI CCCAGC 4 cut(s) 311, 592, 1569, 1599
BsgI GTGCAG 2 cut(s) 160, 899
Bsh1236I CGCG 4 cut(s) 527, 977, 1028, 2075
Bsh1285I CGRYCG 4 cut(s) 226, 283, 1947, 2079
BshFI GGCC 8 cut(s) 20, 153, 282, 478, 942, 1043, 1946, 2078
BshNI GGYRCC 3 cut(s) 1094, 1820, 2035
BshVI ATCGAT 1 cut(s) 470
BsiEI CGRYCG 4 cut(s) 226, 283, 1947, 2079
BsiHKAI GWGCWC 1 cut(s) 1040
BsiHKCI CYCGRG 1 cut(s) 455
BsiSI CCGG 5 cut(s) 53, 645, 1316, 1544, 2093
BslFI GGGAC 7 cut(s) 43, 113, 146, 728, 1520, 1862, 1921
BsmAI GTCTC 6 cut(s) 437, 602, 648, 1170, 1254, 2104
BsmBI CGTCTC 2 cut(s) 437, 648
BsmFI GGGAC 7 cut(s) 43, 113, 146, 728, 1520, 1862, 1921
BsmI GAATGC 1 cut(s) 694
BsnI GGCC 8 cut(s) 20, 153, 282, 478, 942, 1043, 1946, 2078
Bso31I GGTCTC 1 cut(s) 602
BsoBI CYCGRG 1 cut(s) 455
Bsp120I GGGCCC 1 cut(s) 476
Bsp1286I GDGCHC 3 cut(s) 480, 1040, 1825
Bsp1407I TGTACA 1 cut(s) 1836
Bsp1720I GCTNAGC 2 cut(s) 1887, 2106
Bsp19I CCATGG 1 cut(s) 2082
BspANI GGCC 8 cut(s) 20, 153, 282, 478, 942, 1043, 1946, 2078
BspCNI CTCAG 8 cut(s) 499, 1141, 1147, 1174, 1180, 1900, 2065, 2119
BspDI ATCGAT 1 cut(s) 470
BspFNI CGCG 4 cut(s) 527, 977, 1028, 2075
BspPI GGATC 6 cut(s) 98, 891, 1077, 1325, 1346, 1991
BspQI GCTCTTC 1 cut(s) 897
BspT107I GGYRCC 3 cut(s) 1094, 1820, 2035
BspTNI GGTCTC 1 cut(s) 602
BsrBI CCGCTC 2 cut(s) 1712, 1731
BsrDI GCAATG 1 cut(s) 1972
BsrGI TGTACA 1 cut(s) 1836
BsrI ACTGG 8 cut(s) 14, 518, 1100, 1280, 1556, 1679, 1821, 1971
BssECI CCNNGG 3 cut(s) 1525, 2073, 2082
BssNI GRCGYC 5 cut(s) 657, 1095, 1121, 2036, 2069
BssT1I CCWWGG 1 cut(s) 2082
Bst2UI CCWGG 3 cut(s) 1309, 1527, 1651
Bst4CI ACNGT 2 cut(s) 925, 968
Bst6I CTCTTC 3 cut(s) 446, 778, 897
BstACI GRCGYC 5 cut(s) 657, 1095, 1121, 2036, 2069
BstAUI TGTACA 1 cut(s) 1836
BstBAI YACGTR 1 cut(s) 835
BstC8I GCNNGC 2 cut(s) 590, 807
BstDEI CTNAG 8 cut(s) 507, 1128, 1134, 1161, 1167, 1887, 2052, 2106
BstDSI CCRYGG 2 cut(s) 2073, 2082
BstF5I GGATG 6 cut(s) 108, 924, 1019, 1512, 1594, 1930
BstFNI CGCG 4 cut(s) 527, 977, 1028, 2075
BstH2I RGCGCY 3 cut(s) 408, 1098, 2039
BstHHI GCGC 7 cut(s) 407, 529, 1030, 1097, 1774, 2011, 2038
BstMAI GTCTC 6 cut(s) 437, 602, 648, 1170, 1254, 2104
BstMCI CGRYCG 4 cut(s) 226, 283, 1947, 2079
BstNI CCWGG 3 cut(s) 1309, 1527, 1651
BstNSI RCATGY 1 cut(s) 1645
BstSCI CCNGG 4 cut(s) 51, 1307, 1525, 1649
BstSLI GKGCMC 2 cut(s) 480, 1825
BstUI CGCG 4 cut(s) 527, 977, 1028, 2075
BstV2I GAAGAC 1 cut(s) 324
BstXI CCANNNNNNTGG 1 cut(s) 637
BstZI CGGCCG 3 cut(s) 280, 1944, 2076
Bsu15I ATCGAT 1 cut(s) 470
BsuRI GGCC 8 cut(s) 20, 153, 282, 478, 942, 1043, 1946, 2078
BsuTUI ATCGAT 1 cut(s) 470
BtgI CCRYGG 2 cut(s) 2073, 2082
BtgZI GCGATG 2 cut(s) 786, 972
BtrI CACGTC 1 cut(s) 789
BtsCI GGATG 6 cut(s) 108, 924, 1019, 1512, 1594, 1930
BtsIMutI CAGTG 2 cut(s) 21, 1287
Cac8I GCNNGC 2 cut(s) 590, 807
CaiI CAGNNNCTG 4 cut(s) 674, 1153, 1214, 2032
CciNI GCGGCCGC 1 cut(s) 2076
CfoI GCGC 7 cut(s) 407, 529, 1030, 1097, 1774, 2011, 2038
Cfr42I CCGCGG 1 cut(s) 2076
ClaI ATCGAT 1 cut(s) 470
CseI GACGC 1 cut(s) 1129
Csp6I GTAC 6 cut(s) 301, 379, 1399, 1549, 1837, 2056
CviAII CATG 9 cut(s) 96, 136, 367, 1246, 1642, 1756, 2006, 2065, 2083
CviQI GTAC 6 cut(s) 301, 379, 1399, 1549, 1837, 2056
DdeI CTNAG 8 cut(s) 507, 1128, 1134, 1161, 1167, 1887, 2052, 2106
DinI GGCGCC 2 cut(s) 1096, 2037
EaeI YGGCCR 5 cut(s) 151, 280, 1041, 1944, 2076
EagI CGGCCG 3 cut(s) 280, 1944, 2076
Eam1104I CTCTTC 3 cut(s) 446, 778, 897
EarI CTCTTC 3 cut(s) 446, 778, 897
EciI GGCGGA 7 cut(s) 119, 562, 717, 867, 1012, 1554, 1851
EclXI CGGCCG 3 cut(s) 280, 1944, 2076
Eco130I CCWWGG 1 cut(s) 2082
Eco24I GRGCYC 1 cut(s) 480
Eco31I GGTCTC 1 cut(s) 602
Eco47I GGWCC 6 cut(s) 4, 355, 783, 829, 979, 1534
Eco52I CGGCCG 3 cut(s) 280, 1944, 2076
Eco57I CTGAAG 3 cut(s) 470, 651, 1124
Eco72I CACGTG 1 cut(s) 835
Eco88I CYCGRG 1 cut(s) 455
EcoO109I RGGNCCY 2 cut(s) 355, 476
EcoRII CCWGG 3 cut(s) 1307, 1525, 1649
EcoT14I CCWWGG 1 cut(s) 2082
EcoT38I GRGCYC 1 cut(s) 480
EgeI GGCGCC 2 cut(s) 1096, 2037
EheI GGCGCC 2 cut(s) 1096, 2037
ErhI CCWWGG 1 cut(s) 2082
Esp3I CGTCTC 2 cut(s) 437, 648
FaeI CATG 9 cut(s) 99, 139, 370, 1249, 1645, 1759, 2009, 2068, 2086
FaqI GGGAC 7 cut(s) 43, 113, 146, 728, 1520, 1862, 1921
FatI CATG 9 cut(s) 95, 135, 366, 1245, 1641, 1755, 2005, 2064, 2082
FauI CCCGC 5 cut(s) 30, 89, 173, 614, 1736
FbaI TGATCA 1 cut(s) 1606
FblI GTMKAC 1 cut(s) 222
FokI GGATG 6 cut(s) 95, 931, 1006, 1499, 1581, 1917
FriOI GRGCYC 1 cut(s) 480
FspBI CTAG 3 cut(s) 1182, 1325, 1868
FspI TGCGCA 1 cut(s) 2010
GlaI GCGC 7 cut(s) 406, 528, 1029, 1096, 1773, 2010, 2037
GsaI CCCAGC 4 cut(s) 315, 596, 1573, 1603
HaeII RGCGCY 3 cut(s) 408, 1098, 2039
HaeIII GGCC 8 cut(s) 20, 153, 282, 478, 942, 1043, 1946, 2078
HapII CCGG 5 cut(s) 53, 645, 1316, 1544, 2093
HgaI GACGC 1 cut(s) 1129
HhaI GCGC 7 cut(s) 407, 529, 1030, 1097, 1774, 2011, 2038
Hin1I GRCGYC 5 cut(s) 657, 1095, 1121, 2036, 2069
Hin1II CATG 9 cut(s) 99, 139, 370, 1249, 1645, 1759, 2009, 2068, 2086
Hin6I GCGC 7 cut(s) 405, 527, 1028, 1095, 1772, 2009, 2036
HinP1I GCGC 7 cut(s) 405, 527, 1028, 1095, 1772, 2009, 2036
HincII GTYRAC 1 cut(s) 223
HindII GTYRAC 1 cut(s) 223
HpaII CCGG 5 cut(s) 53, 645, 1316, 1544, 2093
HphI GGTGA 2 cut(s) 283, 334
Hpy166II GTNNAC 6 cut(s) 223, 736, 786, 832, 1387, 1837
Hpy188III TCNNGA 6 cut(s) 485, 520, 599, 651, 1274, 1388
Hpy8I GTNNAC 6 cut(s) 223, 736, 786, 832, 1387, 1837
HpyAV CCTTC 5 cut(s) 298, 1731, 1776, 1962, 2058
HpyCH4III ACNGT 2 cut(s) 925, 968
HpyCH4IV ACGT 7 cut(s) 192, 657, 732, 788, 834, 1401, 2069
HpyCH4V TGCA 8 cut(s) 31, 141, 684, 880, 1301, 1375, 1408, 1927
HpyF3I CTNAG 8 cut(s) 507, 1128, 1134, 1161, 1167, 1887, 2052, 2106
HpySE526I ACGT 7 cut(s) 192, 657, 732, 788, 834, 1401, 2069
Hsp92I GRCGYC 5 cut(s) 657, 1095, 1121, 2036, 2069
Hsp92II CATG 9 cut(s) 99, 139, 370, 1249, 1645, 1759, 2009, 2068, 2086
HspAI GCGC 7 cut(s) 405, 527, 1028, 1095, 1772, 2009, 2036
KasI GGCGCC 2 cut(s) 1094, 2035
Ksp22I TGATCA 1 cut(s) 1606
KspI CCGCGG 1 cut(s) 2076
LguI GCTCTTC 1 cut(s) 897
LmnI GCTCC 6 cut(s) 83, 132, 312, 1717, 1736, 1864
LweI GCATC 6 cut(s) 747, 1028, 1362, 1900, 1914, 2133
MaeI CTAG 3 cut(s) 1182, 1325, 1868
MaeII ACGT 7 cut(s) 192, 657, 732, 788, 834, 1401, 2069
MaeIII GTNAC 2 cut(s) 340, 1223
MbiI CCGCTC 2 cut(s) 1712, 1731
MboII GAAGA 6 cut(s) 329, 433, 765, 851, 914, 1947
MfeI CAATTG 1 cut(s) 583
MhlI GDGCHC 3 cut(s) 480, 1040, 1825
MluCI AATT 9 cut(s) 583, 634, 679, 1266, 1337, 1415, 1487, 1800, 1901
Mly113I GGCGCC 2 cut(s) 1095, 2036
MlyI GAGTC 9 cut(s) 59, 65, 86, 262, 467, 548, 563, 635, 1144
MmeI TCCRAC 2 cut(s) 276, 591
MseI TTAA 2 cut(s) 332, 1340
MslI CAYNNNNRTG 1 cut(s) 1922
MspA1I CMGCKG 3 cut(s) 28, 402, 2075
MspI CCGG 5 cut(s) 53, 645, 1316, 1544, 2093
MspR9I CCNGG 4 cut(s) 53, 1309, 1527, 1651
MunI CAATTG 1 cut(s) 583
Mva1269I GAATGC 1 cut(s) 694
MvaI CCWGG 3 cut(s) 1309, 1527, 1651
MvnI CGCG 4 cut(s) 527, 977, 1028, 2075
NarI GGCGCC 2 cut(s) 1095, 2036
NciI CCSGG 1 cut(s) 53
NcoI CCATGG 1 cut(s) 2082
NlaIII CATG 9 cut(s) 99, 139, 370, 1249, 1645, 1759, 2009, 2068, 2086
NmuCI GTSAC 2 cut(s) 340, 1223
NotI GCGGCCGC 1 cut(s) 2076
NsbI TGCGCA 1 cut(s) 2010
NspI RCATGY 1 cut(s) 1645
PciSI GCTCTTC 1 cut(s) 897
PcsI WCGNNNNNNNCGW 9 cut(s) 198, 219, 357, 378, 467, 729, 738, 995, 1083
PctI GAATGC 1 cut(s) 694
PleI GAGTC 9 cut(s) 59, 64, 86, 262, 466, 547, 563, 635, 1144
PluTI GGCGCC 2 cut(s) 1098, 2039
PmaCI CACGTG 1 cut(s) 835
PmlI CACGTG 1 cut(s) 835
PpsI GAGTC 9 cut(s) 59, 64, 86, 262, 466, 547, 563, 635, 1144
Ppu21I YACGTR 1 cut(s) 835
PpuMI RGGWCCY 1 cut(s) 355
Psp5II RGGWCCY 1 cut(s) 355
Psp6I CCWGG 3 cut(s) 1307, 1525, 1649
PspCI CACGTG 1 cut(s) 835
PspFI CCCAGC 4 cut(s) 311, 592, 1569, 1599
PspGI CCWGG 3 cut(s) 1307, 1525, 1649
PspOMI GGGCCC 1 cut(s) 476
PspPPI RGGWCCY 1 cut(s) 355
PstNI CAGNNNCTG 4 cut(s) 674, 1153, 1214, 2032
RsaI GTAC 6 cut(s) 302, 380, 1400, 1550, 1838, 2057
RsaNI GTAC 6 cut(s) 301, 379, 1399, 1549, 1837, 2056
RseI CAYNNNNRTG 1 cut(s) 1922
SacII CCGCGG 1 cut(s) 2076
SalI GTCGAC 1 cut(s) 221
SapI GCTCTTC 1 cut(s) 897
SaqAI TTAA 2 cut(s) 332, 1340
SchI GAGTC 9 cut(s) 59, 65, 86, 262, 467, 548, 563, 635, 1144
ScrFI CCNGG 4 cut(s) 53, 1309, 1527, 1651
SduI GDGCHC 3 cut(s) 480, 1040, 1825
SfaNI GCATC 6 cut(s) 747, 1028, 1362, 1900, 1914, 2133
SfoI GGCGCC 2 cut(s) 1096, 2037
Sfr303I CCGCGG 1 cut(s) 2076
SgrBI CCGCGG 1 cut(s) 2076
SinI GGWCC 6 cut(s) 4, 355, 783, 829, 979, 1534
SmiMI CAYNNNNRTG 1 cut(s) 1922
SmlI CTYRAG 2 cut(s) 296, 761
SmoI CTYRAG 2 cut(s) 296, 761
Sse9I AATT 9 cut(s) 583, 634, 679, 1266, 1337, 1415, 1487, 1800, 1901
SspDI GGCGCC 2 cut(s) 1094, 2035
SspMI CTAG 3 cut(s) 1182, 1325, 1868
StyD4I CCNGG 4 cut(s) 51, 1307, 1525, 1649
StyI CCWWGG 1 cut(s) 2082
TaaI ACNGT 2 cut(s) 925, 968
TaiI ACGT 7 cut(s) 195, 660, 735, 791, 837, 1404, 2072
TaqII GACCGA 1 cut(s) 1522
TasI AATT 9 cut(s) 583, 634, 679, 1266, 1337, 1415, 1487, 1800, 1901
TatI WGTACW 1 cut(s) 1836
Tru1I TTAA 2 cut(s) 332, 1340
Tru9I TTAA 2 cut(s) 332, 1340
TscAI CASTG 2 cut(s) 21, 1287
TseFI GTSAC 2 cut(s) 340, 1223
Tsp45I GTSAC 2 cut(s) 340, 1223
TspDTI ATGAA 2 cut(s) 975, 1998
TspGWI ACGGA 5 cut(s) 328, 419, 568, 730, 1923
TspRI CASTG 2 cut(s) 21, 1287
VpaK11BI GGWCC 6 cut(s) 4, 355, 783, 829, 979, 1534
XceI RCATGY 1 cut(s) 1645
XcmI CCANNNNNNNNNTGG 3 cut(s) 839, 1012, 1813
XmiI GTMKAC 1 cut(s) 222
XspI CTAG 3 cut(s) 1182, 1325, 1868
ZraI GACGTC 2 cut(s) 658, 2070
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.