pycom16g03580

endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0014499
Physical Location & Seq
Reverse (-)
2210354 .. 2212670
2317 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 861 bp
ATGGAGTGTGGCAGGATCCTGATTGTTATTGCCTTGTGCTCGCTGCTCCTTTTTCCCGTGATTCATGGCTGGAGCAATGATGGGCATGTCACAGTCTGCAGAATTGCTCAGTCTAGGCTGAGCAAGGCGGCGGCGGATGCGGTGAAGCAACTGCTTCCAGACTATGCAGAGAATGACTGGAGCAGCCTGTGCATATGGGCAGATCGTGTCAAGTTTATTTTTCCCTGGTCATCTGCCCTTCACTACATTAACACTCCTGAAGTTTGCAGTTACCAGTACAGTAGGGACTGCAAAGATGAGGATGGAGAGAAGGGGCGTTGTGTTGCAGGGGCAATTAACAATTACACCACTCAGCTTCTGACCTACAATAGCCAGTCGTCTCAACCTAAATATAACCTCACGCAGGCATTTCTATTTCTTTCCCATTTCATAGGAGACATCCATCAGCCTCTGCATGTTGGTTTTGCTTCGGACAAGGGAGGCAACACAATTGACGTTCACTGGTATACGAGGAAGCAAAATCTTCACCATATTTGGGATGACAACATAATTGAGACGGCGGAAGAGAGGTTTTACGATTCCAATGTCGAAGAACTAATTGACGCAATTAAACAGAACATTACGACTGAATGGTCAGATCAAGTTAAGGGTTGGGAGACCTGCGGCCTAAACAAGAAAGCATGCCCCAACGTATATGCGTCTGAAGGTATCAAAGCGGCTTGTGATTGGGCATACAAGGGTGCAGATGAAGGTTCAGTGCTAGAAGATGAATACTTCCTCTCCCGGCTACCAATAGTCCATAAGCGGTTAGCTCAAGGTGGTGTGCGTTTAGCAGCTACCCTCAACCGCATCTTCGGGTGA

Protein Analysis

287

Amino Acids

32.31

Weight (kDa)

5.67

Isoelectric Point (pI)

43.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1-P1_nuclease PF02265 24 - 285 5.4e-84 S1/P1 Nuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014499)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68290
fragaria_vesca FvH4_4g27190
malus_domestica MD13G1040300.v1.1 MD16G1041300.v1.1
prunus_persica Prupe.1G312700_v2.0.a1
pyrus_communis pycom16g03580
rosa_chinensis RchiOBHm_Chr4g0434991
rosa_laevigata RLG00000006631
rosa_multiflora Rmu_sc0000400.1_g000035 Rmu_sc0023421.1_g000001
rosa_roxburghii Rroxscaffold_5G00376160
rosa_rugosa Rorug04G0281500 Rorug04G0281500
rosa_samantha Rh4AG336900 Rh4BG344800 Rh4DG339300
rosa_wichuraiana Rw4G029370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 631
Acc36I ACCTGC 1 cut(s) 668
AccI GTMKAC 1 cut(s) 506
AciI CCGC 9 cut(s) 128, 131, 134, 140, 560, 663, 716, 805, 847
AclWI GGATC 2 cut(s) 10, 23
AcuI CTGAAG 2 cut(s) 279, 723
AfaI GTAC 1 cut(s) 278
AfiI CCNNNNNNNGG 2 cut(s) 403, 535
AjnI CCWGG 1 cut(s) 224
AluBI AGCT 3 cut(s) 355, 812, 836
AluI AGCT 3 cut(s) 355, 812, 836
Alw21I GWGCWC 1 cut(s) 41
Alw26I GTCTC 4 cut(s) 384, 429, 548, 650
AlwI GGATC 2 cut(s) 10, 23
AlwNI CAGNNNCTG 2 cut(s) 358, 451
AoxI GGCC 1 cut(s) 664
ApeKI GCWGC 3 cut(s) 43, 183, 833
AsuC2I CCSGG 1 cut(s) 784
AsuHPI GGTGA 2 cut(s) 154, 518
BamHI GGATCC 1 cut(s) 15
Bbv12I GWGCWC 1 cut(s) 41
BbvI GCAGC 3 cut(s) 30, 195, 845
BccI CCATC 3 cut(s) 74, 296, 450
BceAI ACGGC 1 cut(s) 573
BciT130I CCWGG 1 cut(s) 226
BcnI CCSGG 1 cut(s) 784
BcoDI GTCTC 4 cut(s) 384, 429, 548, 650
BfaI CTAG 2 cut(s) 114, 761
BfmI CTRYAG 1 cut(s) 97
BfuAI ACCTGC 1 cut(s) 668
BisI GCNGC 7 cut(s) 44, 129, 132, 184, 664, 717, 834
BlpI GCTNAGC 1 cut(s) 119
BlsI GCNGC 7 cut(s) 45, 130, 133, 185, 665, 718, 835
Bme1390I CCNGG 2 cut(s) 226, 784
BmiI GGNNCC 1 cut(s) 17
BmrFI CCNGG 2 cut(s) 226, 784
BmsI GCATC 1 cut(s) 127
BpmI CTGGAG 2 cut(s) 91, 199
Bpu1102I GCTNAGC 1 cut(s) 119
BpuEI CTTGAG 1 cut(s) 798
BpuMI CCSGG 1 cut(s) 784
BsaI GGTCTC 1 cut(s) 650
BsaJI CCNNGG 1 cut(s) 224
BsaXI ACNNNNNCTCC 2 cut(s) 764, 794
Bsc4I CCNNNNNNNGG 2 cut(s) 403, 535
Bse1I ACTGG 4 cut(s) 182, 274, 373, 506
Bse3DI GCAATG 1 cut(s) 82
BseBI CCWGG 1 cut(s) 226
BseDI CCNNGG 1 cut(s) 224
BseGI GGATG 4 cut(s) 142, 307, 438, 544
BseLI CCNNNNNNNGG 2 cut(s) 403, 535
BseMI GCAATG 1 cut(s) 82
BseMII CTCAG 3 cut(s) 110, 122, 365
BseNI ACTGG 4 cut(s) 182, 274, 373, 506
BseXI GCAGC 3 cut(s) 30, 195, 845
BsgI GTGCAG 1 cut(s) 762
BshFI GGCC 1 cut(s) 666
BsiHKAI GWGCWC 1 cut(s) 41
BsiSI CCGG 1 cut(s) 784
BslFI GGGAC 1 cut(s) 299
BslI CCNNNNNNNGG 2 cut(s) 403, 535
BsmAI GTCTC 4 cut(s) 384, 429, 548, 650
BsmBI CGTCTC 2 cut(s) 384, 548
BsmFI GGGAC 1 cut(s) 299
BsnI GGCC 1 cut(s) 666
Bso31I GGTCTC 1 cut(s) 650
Bsp1286I GDGCHC 1 cut(s) 41
Bsp143I GATC 3 cut(s) 15, 202, 637
Bsp1720I GCTNAGC 1 cut(s) 119
BspACI CCGC 9 cut(s) 128, 131, 134, 140, 560, 663, 716, 805, 847
BspANI GGCC 1 cut(s) 666
BspCNI CTCAG 3 cut(s) 111, 121, 364
BspLI GGNNCC 1 cut(s) 17
BspMAI CTGCAG 1 cut(s) 101
BspMI ACCTGC 1 cut(s) 668
BspPI GGATC 2 cut(s) 10, 23
BspTNI GGTCTC 1 cut(s) 650
BsrDI GCAATG 1 cut(s) 82
BsrI ACTGG 4 cut(s) 182, 274, 373, 506
BssECI CCNNGG 1 cut(s) 224
BssMI GATC 3 cut(s) 15, 202, 637
BssNAI GTATAC 1 cut(s) 507
Bst1107I GTATAC 1 cut(s) 507
Bst2UI CCWGG 1 cut(s) 226
Bst4CI ACNGT 2 cut(s) 94, 281
Bst6I CTCTTC 1 cut(s) 558
BstAPI GCANNNNNTGC 1 cut(s) 189
BstC8I GCNNGC 3 cut(s) 41, 405, 682
BstDEI CTNAG 3 cut(s) 108, 119, 351
BstENI CCTNNNNNAGG 1 cut(s) 401
BstF5I GGATG 4 cut(s) 142, 307, 438, 544
BstKTI GATC 3 cut(s) 18, 205, 640
BstMAI GTCTC 4 cut(s) 384, 429, 548, 650
BstMBI GATC 3 cut(s) 15, 202, 637
BstMWI GCNNNNNNNGC 2 cut(s) 137, 189
BstNI CCWGG 1 cut(s) 226
BstNSI RCATGY 3 cut(s) 89, 458, 684
BstSCI CCNGG 2 cut(s) 224, 782
BstSFI CTRYAG 1 cut(s) 97
BstV1I GCAGC 3 cut(s) 30, 195, 845
BstX2I RGATCY 1 cut(s) 15
BstYI RGATCY 1 cut(s) 15
BstZ17I GTATAC 1 cut(s) 507
BsuRI GGCC 1 cut(s) 666
BtsCI GGATG 4 cut(s) 142, 307, 438, 544
BtsIMutI CAGTG 2 cut(s) 499, 762
BveI ACCTGC 1 cut(s) 668
Cac8I GCNNGC 3 cut(s) 41, 405, 682
CaiI CAGNNNCTG 2 cut(s) 358, 451
CseI GACGC 2 cut(s) 611, 687
Csp6I GTAC 1 cut(s) 277
CviAII CATG 4 cut(s) 65, 86, 455, 681
CviQI GTAC 1 cut(s) 277
DdeI CTNAG 3 cut(s) 108, 119, 351
DpnI GATC 3 cut(s) 17, 204, 639
DpnII GATC 3 cut(s) 15, 202, 637
DrdI GACNNNNNNGTC 1 cut(s) 631
DseDI GACNNNNNNGTC 1 cut(s) 631
Eam1104I CTCTTC 1 cut(s) 558
EarI CTCTTC 1 cut(s) 558
EciI GGCGGA 2 cut(s) 149, 575
Eco31I GGTCTC 1 cut(s) 650
Eco57I CTGAAG 2 cut(s) 279, 723
EcoNI CCTNNNNNAGG 1 cut(s) 401
EcoRII CCWGG 1 cut(s) 224
Esp3I CGTCTC 2 cut(s) 384, 548
FaeI CATG 4 cut(s) 68, 89, 458, 684
FaqI GGGAC 1 cut(s) 299
FatI CATG 4 cut(s) 64, 85, 454, 680
FauNDI CATATG 1 cut(s) 194
FblI GTMKAC 1 cut(s) 506
Fnu4HI GCNGC 7 cut(s) 44, 129, 132, 184, 664, 717, 834
FokI GGATG 4 cut(s) 149, 314, 425, 551
Fsp4HI GCNGC 7 cut(s) 44, 129, 132, 184, 664, 717, 834
FspBI CTAG 2 cut(s) 114, 761
GluI GCNGC 7 cut(s) 44, 129, 132, 184, 664, 717, 834
GsuI CTGGAG 2 cut(s) 91, 199
HaeIII GGCC 1 cut(s) 666
HapII CCGG 1 cut(s) 784
HgaI GACGC 2 cut(s) 611, 687
Hin1II CATG 4 cut(s) 68, 89, 458, 684
HinfI GANTC 2 cut(s) 61, 578
HpaII CCGG 1 cut(s) 784
HphI GGTGA 2 cut(s) 154, 518
Hpy166II GTNNAC 2 cut(s) 499, 507
Hpy188I TCNGA 4 cut(s) 360, 472, 637, 703
Hpy188III TCNNGA 3 cut(s) 19, 158, 257
Hpy8I GTNNAC 2 cut(s) 499, 507
HpyAV CCTTC 4 cut(s) 248, 304, 698, 743
HpyCH4III ACNGT 2 cut(s) 94, 281
HpyCH4IV ACGT 2 cut(s) 495, 690
HpyCH4V TGCA 8 cut(s) 99, 167, 192, 267, 291, 326, 454, 743
HpyF10VI GCNNNNNNNGC 2 cut(s) 137, 189
HpyF3I CTNAG 3 cut(s) 108, 119, 351
HpySE526I ACGT 2 cut(s) 495, 690
Hsp92II CATG 4 cut(s) 68, 89, 458, 684
Kzo9I GATC 3 cut(s) 15, 202, 637
LmnI GCTCC 3 cut(s) 51, 72, 180
Lsp1109I GCAGC 3 cut(s) 30, 195, 845
LweI GCATC 1 cut(s) 127
MaeI CTAG 2 cut(s) 114, 761
MaeII ACGT 2 cut(s) 495, 690
MaeIII GTNAC 2 cut(s) 88, 269
MalI GATC 3 cut(s) 17, 204, 639
MboI GATC 3 cut(s) 15, 202, 637
MboII GAAGA 5 cut(s) 515, 575, 602, 776, 844
MfeI CAATTG 1 cut(s) 489
MflI RGATCY 1 cut(s) 15
MhlI GDGCHC 1 cut(s) 41
MluCI AATT 7 cut(s) 102, 333, 340, 489, 549, 597, 606
MnlI CCTC 8 cut(s) 292, 407, 459, 473, 504, 561, 788, 851
MseI TTAA 4 cut(s) 249, 336, 609, 645
MspI CCGG 1 cut(s) 784
MspR9I CCNGG 2 cut(s) 226, 784
MunI CAATTG 1 cut(s) 489
MvaI CCWGG 1 cut(s) 226
MwoI GCNNNNNNNGC 2 cut(s) 137, 189
NciI CCSGG 1 cut(s) 784
NdeI CATATG 1 cut(s) 194
NdeII GATC 3 cut(s) 15, 202, 637
NlaIII CATG 4 cut(s) 68, 89, 458, 684
NlaIV GGNNCC 1 cut(s) 17
NmuCI GTSAC 1 cut(s) 88
NspI RCATGY 3 cut(s) 89, 458, 684
PaeI GCATGC 1 cut(s) 684
PfeI GAWTC 2 cut(s) 61, 578
PkrI GCNGC 7 cut(s) 45, 130, 133, 185, 665, 718, 835
Psp6I CCWGG 1 cut(s) 224
PspGI CCWGG 1 cut(s) 224
PspN4I GGNNCC 1 cut(s) 17
PstI CTGCAG 1 cut(s) 101
PstNI CAGNNNCTG 2 cut(s) 358, 451
PsuI RGATCY 1 cut(s) 15
RsaI GTAC 1 cut(s) 278
RsaNI GTAC 1 cut(s) 277
SaqAI TTAA 4 cut(s) 249, 336, 609, 645
SatI GCNGC 7 cut(s) 44, 129, 132, 184, 664, 717, 834
Sau3AI GATC 3 cut(s) 15, 202, 637
ScrFI CCNGG 2 cut(s) 226, 784
SduI GDGCHC 1 cut(s) 41
SfaNI GCATC 1 cut(s) 127
SfcI CTRYAG 1 cut(s) 97
SmlI CTYRAG 1 cut(s) 813
SmoI CTYRAG 1 cut(s) 813
SphI GCATGC 1 cut(s) 684
Sse9I AATT 7 cut(s) 102, 333, 340, 489, 549, 597, 606
SsiI CCGC 9 cut(s) 128, 131, 134, 140, 560, 663, 716, 805, 847
SspMI CTAG 2 cut(s) 114, 761
StyD4I CCNGG 2 cut(s) 224, 782
TaaI ACNGT 2 cut(s) 94, 281
TaiI ACGT 2 cut(s) 498, 693
TaqI TCGA 1 cut(s) 588
TasI AATT 7 cut(s) 102, 333, 340, 489, 549, 597, 606
TatI WGTACW 1 cut(s) 276
TauI GCSGC 4 cut(s) 131, 134, 666, 719
TfiI GAWTC 2 cut(s) 61, 578
Tru1I TTAA 4 cut(s) 249, 336, 609, 645
Tru9I TTAA 4 cut(s) 249, 336, 609, 645
TscAI CASTG 2 cut(s) 506, 762
TseFI GTSAC 1 cut(s) 88
TseI GCWGC 3 cut(s) 43, 183, 833
Tsp45I GTSAC 1 cut(s) 88
TspDTI ATGAA 4 cut(s) 53, 418, 762, 783
TspRI CASTG 2 cut(s) 506, 762
XagI CCTNNNNNAGG 1 cut(s) 401
XceI RCATGY 3 cut(s) 89, 458, 684
XmiI GTMKAC 1 cut(s) 506
XspI CTAG 2 cut(s) 114, 761
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.