pycom16g05140

Squamosa promoter-binding-like protein 6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0005916
Physical Location & Seq
Forward (+)
3325871 .. 3328292
2422 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 822 bp
ATGGAGTCCTGGAGTTTTGGTTCAGAAGAGAAAGGGTTTTTGTTTCCGGAAGAAATCGATATACCAACTGATGCATTTTCAAGGAATACTAGAAAAGCATTCCTGGACTTGGATGACAAAACTTGCTTTAATTTTGGCAAGGAATCAGTTGACAGCGTGGGGTTCATGGATTTGGGGTTCCCTGATATTAACCAAGGAGTGGAAATCTTGAATGGTGTGGTTTGTAATGATTCTAGTTATAATTCTGTAGCTTCACCGAGTTGTTTGATTAGTTCAAATTCATCTTTTGGTGAGGGAGAATCCGGGTCGAAGTTTTCAGGCCCTGTGACTGAATGCAGTAGTATGGATTCATCAATGATTGCTTTGAAGCTAGGGAGCTTGGCTGATAGCAGAGGTGGGCAGAATATTCAGCATTCGATTTCTACAGAGAGGTCAAAAAAAGCTGGCAATGGCCCGAGGAGTTTGTACCATCCCGCTCCCACCTGCCAGGTACATGGGTGTAACATGGATCTTACCTTCTCAAAGGATTACCACAAGAGGCATAGGGTTTGTGATGCTCACTCCAAGACTGCCGTAGTGATTGTTAATGGAATCGAGCAGCGGTTTTGCCAGCAGTGCAGCAGGTTTCATCTTCTTGCTGAATTTGATGATGTGAAGCGCAGTTGTCGTAGGCGCCTAGCTGGCCACAACCTTCGCCGAAGGAAGCCTCTACTAGATACCCTCGATTGTAAACCGCATAAGTTGATCGATCCGGACATGTCAAGGAAACGGGTATATGGGAAGCTCCTTTCCGAAGGGCACACCCTCTGTTTTTTCGGATAA

Protein Analysis

274

Amino Acids

30.32

Weight (kDa)

7.56

Isoelectric Point (pI)

56.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SBP PF03110 162 - 235 1.7e-31 SBP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 240
AarI CACCTGC 1 cut(s) 491
Acc36I ACCTGC 2 cut(s) 491, 612
AccB1I GGYRCC 1 cut(s) 672
AccB7I CCANNNNNTGG 1 cut(s) 199
AccBSI CCGCTC 1 cut(s) 476
AccIII TCCGGA 2 cut(s) 46, 751
AciI CCGC 3 cut(s) 474, 601, 734
AclWI GGATC 2 cut(s) 516, 743
AcoI YGGCCR 1 cut(s) 682
AcsI RAATTY 2 cut(s) 277, 641
AcyI GRCGYC 1 cut(s) 673
AfaI GTAC 2 cut(s) 467, 492
AfiI CCNNNNNNNGG 2 cut(s) 109, 199
AflIII ACRYGT 1 cut(s) 756
AgsI TTSAA 4 cut(s) 81, 211, 276, 367
AjnI CCWGG 3 cut(s) 8, 102, 486
AloI GAACNNNNNNTCC 3 cut(s) 36, 161, 193
AluBI AGCT 6 cut(s) 251, 370, 378, 443, 680, 784
AluI AGCT 6 cut(s) 251, 370, 378, 443, 680, 784
AlwI GGATC 2 cut(s) 516, 743
AlwNI CAGNNNCTG 1 cut(s) 323
Ama87I CYCGRG 1 cut(s) 454
Aor13HI TCCGGA 2 cut(s) 46, 751
AoxI GGCC 3 cut(s) 319, 451, 682
ApeKI GCWGC 2 cut(s) 598, 618
ApoI RAATTY 2 cut(s) 277, 641
AspLEI GCGC 2 cut(s) 660, 675
AspS9I GGNCC 2 cut(s) 320, 452
AsuC2I CCSGG 1 cut(s) 304
AsuHPI GGTGA 2 cut(s) 246, 302
AvaI CYCGRG 1 cut(s) 454
BaeGI GKGCMC 1 cut(s) 801
BaeI ACNNNNGTAYC 2 cut(s) 482, 515
BalI TGGCCA 1 cut(s) 684
BanI GGYRCC 1 cut(s) 672
BbvI GCAGC 2 cut(s) 610, 630
BccI CCATC 1 cut(s) 477
BceAI ACGGC 1 cut(s) 557
BciT130I CCWGG 3 cut(s) 10, 104, 488
BcnI CCSGG 1 cut(s) 304
BfaI CTAG 5 cut(s) 90, 234, 371, 677, 713
BfmI CTRYAG 2 cut(s) 246, 423
BfoI RGCGCY 1 cut(s) 676
BfuAI ACCTGC 2 cut(s) 491, 612
BglI GCCNNNNNGGC 1 cut(s) 681
BisI GCNGC 2 cut(s) 599, 619
BlsI GCNGC 2 cut(s) 600, 620
Bme1390I CCNGG 4 cut(s) 10, 104, 304, 488
BmeT110I CYCGRG 1 cut(s) 454
BmgT120I GGNCC 2 cut(s) 320, 452
BmiI GGNNCC 2 cut(s) 179, 674
BmrFI CCNGG 4 cut(s) 10, 104, 304, 488
BmsI GCATC 2 cut(s) 61, 544
BpmI CTGGAG 1 cut(s) 31
BpuMI CCSGG 1 cut(s) 304
Bsa29I ATCGAT 2 cut(s) 57, 747
BsaHI GRCGYC 1 cut(s) 673
BsaJI CCNNGG 2 cut(s) 193, 455
BsaWI WCCGGW 2 cut(s) 46, 751
BsaXI ACNNNNNCTCC 1 cut(s) 34
Bsc4I CCNNNNNNNGG 2 cut(s) 109, 199
Bse3DI GCAATG 1 cut(s) 454
BseAI TCCGGA 2 cut(s) 46, 751
BseBI CCWGG 3 cut(s) 10, 104, 488
BseCI ATCGAT 2 cut(s) 57, 747
BseDI CCNNGG 2 cut(s) 193, 455
BseGI GGATG 2 cut(s) 118, 469
BseLI CCNNNNNNNGG 2 cut(s) 109, 199
BseMI GCAATG 1 cut(s) 454
BseRI GAGGAG 1 cut(s) 472
BseSI GKGCMC 1 cut(s) 801
BseXI GCAGC 2 cut(s) 610, 630
BsgI GTGCAG 1 cut(s) 637
BshFI GGCC 3 cut(s) 321, 453, 684
BshNI GGYRCC 1 cut(s) 672
BshVI ATCGAT 2 cut(s) 57, 747
BsiHKCI CYCGRG 1 cut(s) 454
BsiSI CCGG 3 cut(s) 47, 303, 752
BslI CCNNNNNNNGG 2 cut(s) 109, 199
BsmI GAATGC 3 cut(s) 98, 338, 412
BsnI GGCC 3 cut(s) 321, 453, 684
BsoBI CYCGRG 1 cut(s) 454
Bsp1286I GDGCHC 1 cut(s) 801
Bsp13I TCCGGA 2 cut(s) 46, 751
Bsp143I GATC 3 cut(s) 508, 744, 748
BspACI CCGC 3 cut(s) 474, 601, 734
BspANI GGCC 3 cut(s) 321, 453, 684
BspDI ATCGAT 2 cut(s) 57, 747
BspEI TCCGGA 2 cut(s) 46, 751
BspLI GGNNCC 2 cut(s) 179, 674
BspMI ACCTGC 2 cut(s) 491, 612
BspPI GGATC 2 cut(s) 516, 743
BspT107I GGYRCC 1 cut(s) 672
BsrBI CCGCTC 1 cut(s) 476
BsrDI GCAATG 1 cut(s) 454
BssECI CCNNGG 2 cut(s) 193, 455
BssMI GATC 3 cut(s) 508, 744, 748
BssNI GRCGYC 1 cut(s) 673
BssT1I CCWWGG 1 cut(s) 193
Bst2UI CCWGG 3 cut(s) 10, 104, 488
Bst6I CTCTTC 1 cut(s) 21
BstACI GRCGYC 1 cut(s) 673
BstC8I GCNNGC 3 cut(s) 445, 611, 682
BstF5I GGATG 2 cut(s) 118, 469
BstH2I RGCGCY 1 cut(s) 676
BstHHI GCGC 2 cut(s) 660, 675
BstKTI GATC 3 cut(s) 511, 747, 751
BstMBI GATC 3 cut(s) 508, 744, 748
BstMWI GCNNNNNNNGC 2 cut(s) 615, 681
BstNI CCWGG 3 cut(s) 10, 104, 488
BstNSI RCATGY 1 cut(s) 760
BstSCI CCNGG 4 cut(s) 8, 102, 302, 486
BstSFI CTRYAG 2 cut(s) 246, 423
BstSLI GKGCMC 1 cut(s) 801
BstV1I GCAGC 2 cut(s) 610, 630
BstX2I RGATCY 1 cut(s) 508
BstXI CCANNNNNNTGG 1 cut(s) 494
BstYI RGATCY 1 cut(s) 508
Bsu15I ATCGAT 2 cut(s) 57, 747
BsuRI GGCC 3 cut(s) 321, 453, 684
BsuTUI ATCGAT 2 cut(s) 57, 747
BtsCI GGATG 2 cut(s) 118, 469
BtsI GCAGTG 1 cut(s) 620
BtsIMutI CAGTG 1 cut(s) 620
BveI ACCTGC 2 cut(s) 491, 612
Cac8I GCNNGC 3 cut(s) 445, 611, 682
CaiI CAGNNNCTG 1 cut(s) 323
CfoI GCGC 2 cut(s) 660, 675
Cfr13I GGNCC 2 cut(s) 320, 452
ClaI ATCGAT 2 cut(s) 57, 747
Csp6I GTAC 2 cut(s) 466, 491
CviAII CATG 4 cut(s) 166, 494, 505, 757
CviQI GTAC 2 cut(s) 466, 491
DinI GGCGCC 1 cut(s) 674
DpnI GATC 3 cut(s) 510, 746, 750
DpnII GATC 3 cut(s) 508, 744, 748
EaeI YGGCCR 1 cut(s) 682
Eam1104I CTCTTC 1 cut(s) 21
EarI CTCTTC 1 cut(s) 21
Eco130I CCWWGG 1 cut(s) 193
Eco88I CYCGRG 1 cut(s) 454
EcoO109I RGGNCCY 1 cut(s) 320
EcoRII CCWGG 3 cut(s) 8, 102, 486
EcoT14I CCWWGG 1 cut(s) 193
EcoT22I ATGCAT 1 cut(s) 76
EgeI GGCGCC 1 cut(s) 674
EheI GGCGCC 1 cut(s) 674
ErhI CCWWGG 1 cut(s) 193
FaeI CATG 4 cut(s) 169, 497, 508, 760
FatI CATG 4 cut(s) 165, 493, 504, 756
FauI CCCGC 1 cut(s) 481
Fnu4HI GCNGC 2 cut(s) 599, 619
FokI GGATG 2 cut(s) 125, 456
Fsp4HI GCNGC 2 cut(s) 599, 619
FspBI CTAG 5 cut(s) 90, 234, 371, 677, 713
GlaI GCGC 2 cut(s) 659, 674
GluI GCNGC 2 cut(s) 599, 619
GsuI CTGGAG 1 cut(s) 31
HaeII RGCGCY 1 cut(s) 676
HaeIII GGCC 3 cut(s) 321, 453, 684
HapII CCGG 3 cut(s) 47, 303, 752
HhaI GCGC 2 cut(s) 660, 675
Hin1I GRCGYC 1 cut(s) 673
Hin1II CATG 4 cut(s) 169, 497, 508, 760
Hin6I GCGC 2 cut(s) 658, 673
HinP1I GCGC 2 cut(s) 658, 673
HincII GTYRAC 1 cut(s) 151
HindII GTYRAC 1 cut(s) 151
HinfI GANTC 6 cut(s) 5, 143, 230, 299, 347, 591
HpaII CCGG 3 cut(s) 47, 303, 752
HphI GGTGA 2 cut(s) 246, 302
Hpy166II GTNNAC 2 cut(s) 151, 731
Hpy188I TCNGA 3 cut(s) 25, 793, 818
Hpy188III TCNNGA 3 cut(s) 47, 208, 752
Hpy8I GTNNAC 2 cut(s) 151, 731
HpyAV CCTTC 4 cut(s) 526, 693, 701, 788
HpyCH4V TGCA 3 cut(s) 74, 336, 618
HpyF10VI GCNNNNNNNGC 2 cut(s) 615, 681
Hsp92I GRCGYC 1 cut(s) 673
Hsp92II CATG 4 cut(s) 169, 497, 508, 760
HspAI GCGC 2 cut(s) 658, 673
KasI GGCGCC 1 cut(s) 672
Kpn2I TCCGGA 2 cut(s) 46, 751
Kzo9I GATC 3 cut(s) 508, 744, 748
LmnI GCTCC 3 cut(s) 375, 481, 789
Lsp1109I GCAGC 2 cut(s) 610, 630
LweI GCATC 2 cut(s) 61, 544
MaeI CTAG 5 cut(s) 90, 234, 371, 677, 713
MaeIII GTNAC 2 cut(s) 325, 500
MalI GATC 3 cut(s) 510, 746, 750
MbiI CCGCTC 1 cut(s) 476
MboI GATC 3 cut(s) 508, 744, 748
MboII GAAGA 3 cut(s) 38, 62, 623
MflI RGATCY 1 cut(s) 508
MhlI GDGCHC 1 cut(s) 801
MlsI TGGCCA 1 cut(s) 684
MluCI AATT 4 cut(s) 130, 241, 277, 641
MluNI TGGCCA 1 cut(s) 684
Mly113I GGCGCC 1 cut(s) 673
MlyI GAGTC 1 cut(s) 14
MnlI CCTC 8 cut(s) 286, 386, 423, 450, 531, 717, 731, 815
Mox20I TGGCCA 1 cut(s) 684
Mph1103I ATGCAT 1 cut(s) 76
MroI TCCGGA 2 cut(s) 46, 751
MscI TGGCCA 1 cut(s) 684
MseI TTAA 3 cut(s) 129, 189, 585
Msp20I TGGCCA 1 cut(s) 684
MspA1I CMGCKG 1 cut(s) 601
MspI CCGG 3 cut(s) 47, 303, 752
MspR9I CCNGG 4 cut(s) 10, 104, 304, 488
Mva1269I GAATGC 3 cut(s) 98, 338, 412
MvaI CCWGG 3 cut(s) 10, 104, 488
MwoI GCNNNNNNNGC 2 cut(s) 615, 681
NarI GGCGCC 1 cut(s) 673
NciI CCSGG 1 cut(s) 304
NdeII GATC 3 cut(s) 508, 744, 748
NlaIII CATG 4 cut(s) 169, 497, 508, 760
NlaIV GGNNCC 2 cut(s) 179, 674
NmuCI GTSAC 1 cut(s) 325
NsiI ATGCAT 1 cut(s) 76
NspI RCATGY 1 cut(s) 760
PaqCI CACCTGC 1 cut(s) 491
PciI ACATGT 1 cut(s) 756
PctI GAATGC 3 cut(s) 98, 338, 412
PfeI GAWTC 5 cut(s) 143, 230, 299, 347, 591
PflMI CCANNNNNTGG 1 cut(s) 199
PfoI TCCNGGA 2 cut(s) 8, 102
PkrI GCNGC 2 cut(s) 600, 620
PleI GAGTC 1 cut(s) 13
PluTI GGCGCC 1 cut(s) 676
PpsI GAGTC 1 cut(s) 13
PscI ACATGT 1 cut(s) 756
PsiI TTATAA 1 cut(s) 240
Psp6I CCWGG 3 cut(s) 8, 102, 486
PspGI CCWGG 3 cut(s) 8, 102, 486
PspN4I GGNNCC 2 cut(s) 179, 674
PspPI GGNCC 2 cut(s) 320, 452
PstNI CAGNNNCTG 1 cut(s) 323
PsuI RGATCY 1 cut(s) 508
RsaI GTAC 2 cut(s) 467, 492
RsaNI GTAC 2 cut(s) 466, 491
SaqAI TTAA 3 cut(s) 129, 189, 585
SatI GCNGC 2 cut(s) 599, 619
Sau3AI GATC 3 cut(s) 508, 744, 748
Sau96I GGNCC 2 cut(s) 320, 452
SchI GAGTC 1 cut(s) 14
ScrFI CCNGG 4 cut(s) 10, 104, 304, 488
SduI GDGCHC 1 cut(s) 801
SfaNI GCATC 2 cut(s) 61, 544
SfcI CTRYAG 2 cut(s) 246, 423
SfoI GGCGCC 1 cut(s) 674
Sse9I AATT 4 cut(s) 130, 241, 277, 641
SsiI CCGC 3 cut(s) 474, 601, 734
SspDI GGCGCC 1 cut(s) 672
SspI AATATT 1 cut(s) 406
SspMI CTAG 5 cut(s) 90, 234, 371, 677, 713
StyD4I CCNGG 4 cut(s) 8, 102, 302, 486
StyI CCWWGG 1 cut(s) 193
TaqI TCGA 6 cut(s) 57, 308, 416, 594, 723, 747
TasI AATT 4 cut(s) 130, 241, 277, 641
TfiI GAWTC 5 cut(s) 143, 230, 299, 347, 591
Tru1I TTAA 3 cut(s) 129, 189, 585
Tru9I TTAA 3 cut(s) 129, 189, 585
TscAI CASTG 1 cut(s) 620
TseFI GTSAC 1 cut(s) 325
TseI GCWGC 2 cut(s) 598, 618
Tsp45I GTSAC 1 cut(s) 325
TspDTI ATGAA 4 cut(s) 154, 270, 339, 617
TspRI CASTG 1 cut(s) 620
Van91I CCANNNNNTGG 1 cut(s) 199
XapI RAATTY 2 cut(s) 277, 641
XceI RCATGY 1 cut(s) 760
XspI CTAG 5 cut(s) 90, 234, 371, 677, 713
Zsp2I ATGCAT 1 cut(s) 76
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.