pycom16g05490

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0007654
Physical Location & Seq
Forward (+)
3539489 .. 3542402
2914 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1305 bp
ATGGGTATAAAAACAAAATTATTTACATTTTTTTCTTCTGATATTTGTCAGTACGAAGGTGCGGTTAAAGAGGATGGAAGAGGGCCTTCGATCTGGGACACCTTTTCACATACTTTTGATGGAACCGGGCAAATTAATCAGGCGGGTGTTGATTATTACAATCGTCTCATCGATGCATTACTAGCCAAAGGAATTGAACCATATGTGACCCTCTATCACTGGGACCTCCCTCAAGCCTTGGAAGACAGATACAACGGGTGGCTCAACCCTCAAATCATAAAGGACTTTGCAACGTACGCGGACACATGCTTTCAAAATTTTGGTGACAGGGTGAAACACTGGATCACATTCAACGAGCCACATACATTTTCTGTACATGGATATGCTTCGGGCCTCCAGGCACCTGGAAGGTGCTCTATCCTGCGTCCACTCTTATGCAGGTCCGGAAACTCTGCAACTGAGCCTTACATAGTTGCTCACAATGTCATCCTGTCTCATGGAACTGTGGCTGATATTTACAAGAGAAAGTATAAGTCAAAACAGCAGGGATCGGTTGGGGCATCATTTGATGTTATCTGGTACGAACCAGAAACAAACTCAACAGAAGACGTCATTGCAACTAGCGTAGCCCAAGAATTTCAGCTTGGCTGGTTTCTCGATCCATTTATTTTTGGGGATTATCCAAGCTCTATGAGAAGTAGGGTTGGGAGCCGGCTGCCAACCTTTTCCAAATCCGAGTCTACTCTAATTAAAGGGTCCTTGGATTTTGTGGGCATTAATCACTACACTACCTTCTATGGTAAAAACGATACCAGGGATTTAATCGGAGGTCTACTTAACGGCAGCCTTTCAGACTCTGGTGCCATTACCCTTCCATTCAAAAACGGGAAACCTATTGGAGATAGGGCGAATTCTATATGGTTATACATAGTCCCAGAGGGGATGAGAAAATTAATGAACTACATTAAGCAAAAGTACGGCAATCCTCCAGTGATTATCACCGAAAATGGCATGGATGACCCGAATAGCCTGTTCATTTCCCTCAAGGACGCTTTAAAGGATACAAAAAGGATTAAATACCACCATGACTATCTTGCAAATTTGCTAGCTTCGATCAAGCAAGACGGCTGCAATGTGAAGGGCTATTTTGCTTGGTCTCTACTGGATAATTGGGAATGGACGGCTGGATTCACTTCTCGATTTGGTCTCTACTTTGTGGATTATAAGGACAAGCTCAAGAGATACCCGAAGGACTCCGTTCAATGGTTCACGAATTTCTTGAATTCTACTCAAACAAGGCTATGA

Protein Analysis

435

Amino Acids

49.28

Weight (kDa)

7.63

Isoelectric Point (pI)

25.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 41 - 429 2.4e-115 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1224
AatII GACGTC 1 cut(s) 612
Acc36I ACCTGC 1 cut(s) 429
AccB1I GGYRCC 2 cut(s) 400, 860
AccI GTMKAC 2 cut(s) 740, 832
AccII CGCG 1 cut(s) 299
AccIII TCCGGA 1 cut(s) 443
AciI CCGC 3 cut(s) 62, 143, 299
AclWI GGATC 3 cut(s) 350, 556, 653
AcsI RAATTY 6 cut(s) 316, 635, 910, 1099, 1273, 1282
AcyI GRCGYC 1 cut(s) 609
AfaI GTAC 5 cut(s) 53, 296, 375, 581, 977
AfiI CCNNNNNNNGG 1 cut(s) 1263
AgsI TTSAA 6 cut(s) 197, 314, 352, 880, 1262, 1282
AjnI CCWGG 3 cut(s) 396, 403, 812
AjuI GAANNNNNNNTTGG 2 cut(s) 627, 659
AluBI AGCT 4 cut(s) 643, 687, 1109, 1234
AluI AGCT 4 cut(s) 643, 687, 1109, 1234
Alw21I GWGCWC 1 cut(s) 416
Alw26I GTCTC 4 cut(s) 170, 498, 1161, 1211
AlwI GGATC 3 cut(s) 350, 556, 653
AlwNI CAGNNNCTG 1 cut(s) 857
Aor13HI TCCGGA 1 cut(s) 443
AoxI GGCC 2 cut(s) 83, 391
ApeKI GCWGC 3 cut(s) 715, 843, 1128
ApoI RAATTY 6 cut(s) 316, 635, 910, 1099, 1273, 1282
AseI ATTAAT 3 cut(s) 135, 777, 953
AspS9I GGNCC 5 cut(s) 83, 223, 391, 441, 756
AsuC2I CCSGG 1 cut(s) 127
AsuHPI GGTGA 3 cut(s) 335, 343, 991
AsuNHI GCTAGC 1 cut(s) 1105
AvaII GGWCC 3 cut(s) 223, 441, 756
BanI GGYRCC 2 cut(s) 400, 860
BbsI GAAGAC 2 cut(s) 249, 612
Bbv12I GWGCWC 1 cut(s) 416
BbvI GCAGC 3 cut(s) 702, 855, 1115
BccI CCATC 2 cut(s) 68, 113
BceAI ACGGC 4 cut(s) 856, 994, 1141, 1197
BciT130I CCWGG 3 cut(s) 398, 405, 814
BciVI GTATCC 1 cut(s) 1054
BcnI CCSGG 1 cut(s) 127
BcoDI GTCTC 4 cut(s) 170, 498, 1161, 1211
BfaI CTAG 3 cut(s) 182, 621, 1106
BfuAI ACCTGC 1 cut(s) 429
BfuI GTATCC 1 cut(s) 1054
BisI GCNGC 3 cut(s) 716, 844, 1129
BlsI GCNGC 3 cut(s) 717, 845, 1130
Bme1390I CCNGG 4 cut(s) 127, 398, 405, 814
Bme18I GGWCC 3 cut(s) 223, 441, 756
BmgT120I GGNCC 5 cut(s) 83, 223, 391, 441, 756
BmiI GGNNCC 6 cut(s) 124, 224, 402, 710, 757, 862
BmrFI CCNGG 4 cut(s) 127, 398, 405, 814
BmrI ACTGGG 1 cut(s) 229
BmsI GCATC 2 cut(s) 163, 569
BmtI GCTAGC 1 cut(s) 1109
BmuI ACTGGG 1 cut(s) 229
BpiI GAAGAC 2 cut(s) 249, 612
BpmI CTGGAG 2 cut(s) 380, 972
BpuEI CTTGAG 3 cut(s) 216, 1028, 1220
BpuMI CCSGG 1 cut(s) 127
Bsa29I ATCGAT 1 cut(s) 171
BsaHI GRCGYC 1 cut(s) 609
BsaI GGTCTC 2 cut(s) 1161, 1211
BsaJI CCNNGG 3 cut(s) 237, 759, 813
BsaWI WCCGGW 1 cut(s) 443
Bsc4I CCNNNNNNNGG 1 cut(s) 1263
Bse118I RCCGGY 1 cut(s) 711
Bse1I ACTGG 4 cut(s) 224, 344, 989, 1167
Bse3DI GCAATG 2 cut(s) 612, 1138
BseAI TCCGGA 1 cut(s) 443
BseBI CCWGG 3 cut(s) 398, 405, 814
BseCI ATCGAT 1 cut(s) 171
BseDI CCNNGG 3 cut(s) 237, 759, 813
BseGI GGATG 4 cut(s) 79, 486, 948, 1021
BseLI CCNNNNNNNGG 1 cut(s) 1263
BseMI GCAATG 2 cut(s) 612, 1138
BseMII CTCAG 1 cut(s) 450
BseNI ACTGG 4 cut(s) 224, 344, 989, 1167
BseXI GCAGC 3 cut(s) 702, 855, 1115
Bsh1236I CGCG 1 cut(s) 299
BshFI GGCC 2 cut(s) 85, 393
BshNI GGYRCC 2 cut(s) 400, 860
BshVI ATCGAT 1 cut(s) 171
BsiHKAI GWGCWC 1 cut(s) 416
BsiSI CCGG 3 cut(s) 126, 444, 712
BsiWI CGTACG 1 cut(s) 294
BslFI GGGAC 3 cut(s) 110, 236, 917
BslI CCNNNNNNNGG 1 cut(s) 1263
BsmAI GTCTC 4 cut(s) 170, 498, 1161, 1211
BsmBI CGTCTC 1 cut(s) 170
BsmFI GGGAC 3 cut(s) 110, 236, 917
BsnI GGCC 2 cut(s) 85, 393
Bso31I GGTCTC 2 cut(s) 1161, 1211
Bsp1286I GDGCHC 1 cut(s) 416
Bsp13I TCCGGA 1 cut(s) 443
Bsp1407I TGTACA 1 cut(s) 373
Bsp143I GATC 5 cut(s) 90, 342, 548, 658, 1113
BspACI CCGC 3 cut(s) 62, 143, 299
BspANI GGCC 2 cut(s) 85, 393
BspCNI CTCAG 1 cut(s) 451
BspDI ATCGAT 1 cut(s) 171
BspEI TCCGGA 1 cut(s) 443
BspFNI CGCG 1 cut(s) 299
BspLI GGNNCC 6 cut(s) 124, 224, 402, 710, 757, 862
BspMI ACCTGC 1 cut(s) 429
BspOI GCTAGC 1 cut(s) 1109
BspPI GGATC 3 cut(s) 350, 556, 653
BspT107I GGYRCC 2 cut(s) 400, 860
BspTNI GGTCTC 2 cut(s) 1161, 1211
BsrDI GCAATG 2 cut(s) 612, 1138
BsrFI RCCGGY 1 cut(s) 711
BsrGI TGTACA 1 cut(s) 373
BsrI ACTGG 4 cut(s) 224, 344, 989, 1167
BssAI RCCGGY 1 cut(s) 711
BssECI CCNNGG 3 cut(s) 237, 759, 813
BssMI GATC 5 cut(s) 90, 342, 548, 658, 1113
BssNI GRCGYC 1 cut(s) 609
BssT1I CCWWGG 2 cut(s) 237, 759
Bst2UI CCWGG 3 cut(s) 398, 405, 814
Bst4CI ACNGT 1 cut(s) 505
Bst6I CTCTTC 1 cut(s) 73
BstACI GRCGYC 1 cut(s) 609
BstAUI TGTACA 1 cut(s) 373
BstC8I GCNNGC 2 cut(s) 713, 1107
BstDEI CTNAG 1 cut(s) 459
BstF5I GGATG 4 cut(s) 79, 486, 948, 1021
BstFNI CGCG 1 cut(s) 299
BstKTI GATC 5 cut(s) 93, 345, 551, 661, 1116
BstMAI GTCTC 4 cut(s) 170, 498, 1161, 1211
BstMBI GATC 5 cut(s) 90, 342, 548, 658, 1113
BstMWI GCNNNNNNNGC 2 cut(s) 182, 296
BstNI CCWGG 3 cut(s) 398, 405, 814
BstNSI RCATGY 1 cut(s) 309
BstSCI CCNGG 4 cut(s) 125, 396, 403, 812
BstUI CGCG 1 cut(s) 299
BstV1I GCAGC 3 cut(s) 702, 855, 1115
BstV2I GAAGAC 2 cut(s) 249, 612
BstXI CCANNNNNNTGG 1 cut(s) 404
Bsu15I ATCGAT 1 cut(s) 171
BsuI GTATCC 1 cut(s) 1054
BsuRI GGCC 2 cut(s) 85, 393
BsuTUI ATCGAT 1 cut(s) 171
BtsCI GGATG 4 cut(s) 79, 486, 948, 1021
BtsIMutI CAGTG 3 cut(s) 217, 337, 996
BveI ACCTGC 1 cut(s) 429
Cac8I GCNNGC 2 cut(s) 713, 1107
CaiI CAGNNNCTG 1 cut(s) 857
Cfr10I RCCGGY 1 cut(s) 711
Cfr13I GGNCC 5 cut(s) 83, 223, 391, 441, 756
ClaI ATCGAT 1 cut(s) 171
CseI GACGC 2 cut(s) 413, 1058
Csp6I GTAC 5 cut(s) 52, 295, 374, 580, 976
CviAII CATG 5 cut(s) 306, 377, 497, 1012, 1085
CviQI GTAC 5 cut(s) 52, 295, 374, 580, 976
DdeI CTNAG 1 cut(s) 459
DpnI GATC 5 cut(s) 92, 344, 550, 660, 1115
DpnII GATC 5 cut(s) 90, 342, 548, 658, 1113
DraI TTTAAA 1 cut(s) 1056
Eam1104I CTCTTC 1 cut(s) 73
EarI CTCTTC 1 cut(s) 73
Eco130I CCWWGG 2 cut(s) 237, 759
Eco31I GGTCTC 2 cut(s) 1161, 1211
Eco47I GGWCC 3 cut(s) 223, 441, 756
EcoO109I RGGNCCY 3 cut(s) 83, 223, 756
EcoRI GAATTC 2 cut(s) 910, 1282
EcoRII CCWGG 3 cut(s) 396, 403, 812
EcoT14I CCWWGG 2 cut(s) 237, 759
EcoT22I ATGCAT 1 cut(s) 178
ErhI CCWWGG 2 cut(s) 237, 759
Esp3I CGTCTC 1 cut(s) 170
FaeI CATG 5 cut(s) 309, 380, 500, 1015, 1088
FalI AAGNNNNNCTT 2 cut(s) 70, 102
FaqI GGGAC 3 cut(s) 110, 236, 917
FatI CATG 5 cut(s) 305, 376, 496, 1011, 1084
FauI CCCGC 1 cut(s) 136
FauNDI CATATG 1 cut(s) 202
FblI GTMKAC 2 cut(s) 740, 832
Fnu4HI GCNGC 3 cut(s) 716, 844, 1129
FokI GGATG 4 cut(s) 86, 473, 955, 1028
Fsp4HI GCNGC 3 cut(s) 716, 844, 1129
FspBI CTAG 3 cut(s) 182, 621, 1106
GluI GCNGC 3 cut(s) 716, 844, 1129
GsuI CTGGAG 2 cut(s) 380, 972
HaeIII GGCC 2 cut(s) 85, 393
HapII CCGG 3 cut(s) 126, 444, 712
HgaI GACGC 2 cut(s) 413, 1058
Hin1I GRCGYC 1 cut(s) 609
Hin1II CATG 5 cut(s) 309, 380, 500, 1015, 1088
HinfI GANTC 4 cut(s) 737, 854, 1188, 1253
HpaII CCGG 3 cut(s) 126, 444, 712
HphI GGTGA 3 cut(s) 335, 343, 991
Hpy166II GTNNAC 4 cut(s) 428, 741, 833, 1269
Hpy188I TCNGA 4 cut(s) 40, 736, 827, 853
Hpy188III TCNNGA 6 cut(s) 444, 656, 1197, 1237, 1270, 1279
Hpy8I GTNNAC 4 cut(s) 428, 741, 833, 1269
HpyAV CCTTC 7 cut(s) 50, 96, 402, 802, 881, 1132, 1243
HpyCH4III ACNGT 1 cut(s) 505
HpyCH4IV ACGT 2 cut(s) 293, 609
HpyCH4V TGCA 7 cut(s) 176, 290, 438, 455, 617, 1097, 1131
HpyF10VI GCNNNNNNNGC 2 cut(s) 182, 296
HpyF3I CTNAG 1 cut(s) 459
HpySE526I ACGT 2 cut(s) 293, 609
Hsp92I GRCGYC 1 cut(s) 609
Hsp92II CATG 5 cut(s) 309, 380, 500, 1015, 1088
Kpn2I TCCGGA 1 cut(s) 443
KroI GCCGGC 1 cut(s) 711
KroNI GCCGGC 1 cut(s) 713
Kzo9I GATC 5 cut(s) 90, 342, 548, 658, 1113
LmnI GCTCC 1 cut(s) 708
Lsp1109I GCAGC 3 cut(s) 702, 855, 1115
LweI GCATC 2 cut(s) 163, 569
MaeI CTAG 3 cut(s) 182, 621, 1106
MaeII ACGT 2 cut(s) 293, 609
MaeIII GTNAC 2 cut(s) 205, 323
MalI GATC 5 cut(s) 92, 344, 550, 660, 1115
MboI GATC 5 cut(s) 90, 342, 548, 658, 1113
MboII GAAGA 4 cut(s) 27, 90, 254, 617
MhlI GDGCHC 1 cut(s) 416
MlyI GAGTC 3 cut(s) 746, 848, 1247
Mph1103I ATGCAT 1 cut(s) 178
MroI TCCGGA 1 cut(s) 443
MroNI GCCGGC 1 cut(s) 711
MslI CAYNNNNRTG 2 cut(s) 381, 433
MspI CCGG 3 cut(s) 126, 444, 712
MspR9I CCNGG 4 cut(s) 127, 398, 405, 814
MvaI CCWGG 3 cut(s) 398, 405, 814
MvnI CGCG 1 cut(s) 299
MwoI GCNNNNNNNGC 2 cut(s) 182, 296
NaeI GCCGGC 1 cut(s) 713
NciI CCSGG 1 cut(s) 127
NdeI CATATG 1 cut(s) 202
NdeII GATC 5 cut(s) 90, 342, 548, 658, 1113
NgoMIV GCCGGC 1 cut(s) 711
NheI GCTAGC 1 cut(s) 1105
NlaIII CATG 5 cut(s) 309, 380, 500, 1015, 1088
NlaIV GGNNCC 6 cut(s) 124, 224, 402, 710, 757, 862
NmuCI GTSAC 2 cut(s) 205, 323
NsiI ATGCAT 1 cut(s) 178
NspI RCATGY 1 cut(s) 309
PdiI GCCGGC 1 cut(s) 713
PfeI GAWTC 1 cut(s) 1188
Pfl23II CGTACG 1 cut(s) 294
PkrI GCNGC 3 cut(s) 717, 845, 1130
PleI GAGTC 3 cut(s) 745, 848, 1247
PpsI GAGTC 3 cut(s) 745, 848, 1247
PpuMI RGGWCCY 2 cut(s) 223, 756
PshBI ATTAAT 3 cut(s) 135, 777, 953
PsiI TTATAA 1 cut(s) 1224
Psp5II RGGWCCY 2 cut(s) 223, 756
Psp6I CCWGG 3 cut(s) 396, 403, 812
PspGI CCWGG 3 cut(s) 396, 403, 812
PspLI CGTACG 1 cut(s) 294
PspN4I GGNNCC 6 cut(s) 124, 224, 402, 710, 757, 862
PspPI GGNCC 5 cut(s) 83, 223, 391, 441, 756
PspPPI RGGWCCY 2 cut(s) 223, 756
PstNI CAGNNNCTG 1 cut(s) 857
RsaI GTAC 5 cut(s) 53, 296, 375, 581, 977
RsaNI GTAC 5 cut(s) 52, 295, 374, 580, 976
RseI CAYNNNNRTG 2 cut(s) 381, 433
SatI GCNGC 3 cut(s) 716, 844, 1129
Sau3AI GATC 5 cut(s) 90, 342, 548, 658, 1113
Sau96I GGNCC 5 cut(s) 83, 223, 391, 441, 756
SchI GAGTC 3 cut(s) 746, 848, 1247
ScrFI CCNGG 4 cut(s) 127, 398, 405, 814
SduI GDGCHC 1 cut(s) 416
SfaNI GCATC 2 cut(s) 163, 569
SinI GGWCC 3 cut(s) 223, 441, 756
SmiMI CAYNNNNRTG 2 cut(s) 381, 433
SmlI CTYRAG 3 cut(s) 231, 1043, 1235
SmoI CTYRAG 3 cut(s) 231, 1043, 1235
SsiI CCGC 3 cut(s) 62, 143, 299
SspMI CTAG 3 cut(s) 182, 621, 1106
StyD4I CCNGG 4 cut(s) 125, 396, 403, 812
StyI CCWWGG 2 cut(s) 237, 759
TaaI ACNGT 1 cut(s) 505
TaiI ACGT 2 cut(s) 296, 612
TaqI TCGA 5 cut(s) 89, 171, 657, 1112, 1198
TatI WGTACW 1 cut(s) 373
TfiI GAWTC 1 cut(s) 1188
TscAI CASTG 3 cut(s) 224, 344, 996
TseFI GTSAC 2 cut(s) 205, 323
TseI GCWGC 3 cut(s) 715, 843, 1128
Tsp45I GTSAC 2 cut(s) 205, 323
TspDTI ATGAA 2 cut(s) 971, 1024
TspGWI ACGGA 1 cut(s) 1246
TspRI CASTG 3 cut(s) 224, 344, 996
VpaK11BI GGWCC 3 cut(s) 223, 441, 756
VspI ATTAAT 3 cut(s) 135, 777, 953
XapI RAATTY 6 cut(s) 316, 635, 910, 1099, 1273, 1282
XceI RCATGY 1 cut(s) 309
XmiI GTMKAC 2 cut(s) 740, 832
XspI CTAG 3 cut(s) 182, 621, 1106
ZraI GACGTC 1 cut(s) 610
Zsp2I ATGCAT 1 cut(s) 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.