Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0001425

Physical Location & Seq

Genomic Coordinates Forward (+)

3795920 .. 3811354

Gene Structure 15435 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1716 bp

ATGAAGTTTGGGAAGGAATTTTCGGTACAGATGGTGCCGGAATGGAGAGTTGCATACATGGATTACGGGTACTTAAAATCCCTCTTAAAAGAACTCCAACTCTCCAAGCATAGAAACAACCCCGATTCACTGGCCGCCGCTTCCACGCCCCACAGTCAAGCCCGAAAGCGGAGGCTCGCGTTGTACAGAACCTTCAGCGGTCTGACAAAAAGCAGGCACGCCCAACAGCCCAGCAGCCCGTCTTCCACCTTTGGTGGTGTCGACATCGAGAGCCAGGCCATTCTTGTTCACTCTGTCAACGAAAACGACTCCGAGAGCTACCAGACGACGTTTCTGATGGCGGCGGAGGAAGGCGGTGTTCAGGAGCTGGAGTACTTCAAGAAGCTCGACAATGAGTTTAATAAAGTTGACAAGTTTTATAGGTCGAAGGTTGACGAGGTGATGAAGGAAGCTGCAGTTCTGAACAAGCAAATGGACGCTTTGATTGCGTTTCAGATCGAAGTGGAGAACCCTCAGAGAATGTTTGATTGGTCGGGGGAGATGACTCGCCTTGCTTCCGATGTTGCCACGTCTACCGCTGCATTGGCTGCCGCCAGCCCACACGGTGCCAGAGCAAGCAGGCGGGTACATGCTATGGATGCGATTGAAGAGCGCTGGTCAAACAGTTCGTGTGATGAAGAAAAAGATGACAAAAAGAGTGGAGACGTTGAGATGGAAGTGATGAAGAAGTCGGAGGGCAGGAGAGGCACTAGGCCTGCGCCACTGGATATACTAGACCGTGTGACAATGAATCACACGGTTGAGACGCCGTGTTCCACCATCAAAGGCTTCCTCAACGTGGCTCCGCAAACGGAGCTCAAGTTCAGCAGGGTTAATCTCAACAAAGTTGAAGAACAGCTGAGGGGAGCTTTCGTTGTATTTTACCAGAAGCTTCGGCTTCTGAAGAGCTACGGATTTTTGAATACGTTGGCTTTCTCGAAGATAATGAAGAAATACGATAAGGTGGCTTCGAGAGATGCATCAAAATCTTACATGAACATGGTGGATAACTCGCACCTTGGCAGCTCCGACGAGGTTACCAAGTTTATGGAGAGGGTTGAAACTACATTCATCAAGCATTTCTCGAACTCAAATCGCCGAAAAGGAATGGCAGTCTTGAGGCCAAAATCGAAGGTGGAAAGACACAGGATAACATTCGCAATGGGTTGCTTTGCTGGCTGCACAGCTGCTCTCATATTGGCCCTTGTTTTGGTCACTCGTGCTCGAAAGATTATGGATAAGTCGGGGAGAAGTCAATACATGGAAAACATGTTCCCCCTATACAGCTTGTTCGGATTCATTATTCTGCACATGCTTATGTATGCCGGAAATATATACTTTTGGCGGCGGTTCAGAGTCAATTACTCTTTCATATTCGGTTTCAAGCAAGGAACCGAGCTGGGATACAGAGAGGTCCTCCTTCTGAGTTTTGGTGTGGCAGTGCTAGCACTAGCATCTGTGCTCTCAAGTCTTGACATGGAGATGGACCCGAAAACCAAAGATTACAAAGAGCTAACCGAACTTCTCCCACTCTTCTTGCTCCTGCTTCTGATTGTGATATTATTATGCCCCTTCAACATCATATATCGCTCGATCGCTACTTCTTCCTTGTTTGTGTGTTCCACAGCATCTGCGCTCCTCTCTATAAGGTCACACTGCCGGATTTCTTCTTGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0005886 GO:0008150 GO:0012505 GO:0016020 GO:0031984 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0048878 GO:0050801 GO:0055062 GO:0055065 GO:0055069 GO:0055076 GO:0055080 GO:0055081 GO:0055083 GO:0065007 GO:0065008 GO:0071944 GO:0072505 GO:0072506 GO:0072507 GO:0098771 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EXS SPX

Protein Analysis

572

Amino Acids

64.92

Weight (kDa)

9.32

Isoelectric Point (pI)

46.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SPX	PF03105	1 - 348	7.7e-83	SPX domain
EXS	PF03124	437 - 546	5.3e-20	EXS family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001425)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G14040 AT1G26730 AT1G26730 AT1G35350 AT1G35350 AT2G03240 AT2G03260 AT2G03260
fragaria_vesca	FvH4_4g21230 FvH4_4g30900 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930 FvH4_4g30930
malus_domestica	MD00G1081000.v1.1 MD00G1081300.v1.1 MD00G1200800.v1.1 MD16G1068000.v1.1
prunus_persica	Prupe.1G278700_v2.0.a1 Prupe.1G278700_v2.0.a1 Prupe.1G278700_v2.0.a1 Prupe.1G278800_v2.0.a1
pyrus_communis	pycom13g06110 pycom13g06120 pycom16g05960
rosa_chinensis	RchiOBHm_Chr4g0439231
rosa_laevigata	RLG00000006286
rosa_multiflora	Rmu_sc0006598.1_g000012 Rmu_sc0007941.1_g000004 Rmu_ssc0000171.1_g000025
rosa_roxburghii	Rroxscaffold_2G00115520 Rroxscaffold_2G00115990 Rroxscaffold_5G00380220
rosa_rugosa	Rorug04G0317100 Rorug04G0317200
rosa_samantha	Rh4AG367700 Rh4BG379600 Rh4CG393900 Rh4DG373900
rosa_wichuraiana	Rw0G005620 Rw0G011630 Rw5G028550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	34, 605
AccI	GTMKAC	2 cut(s)	261, 572
AccII	CGCG	1 cut(s)	179
AcoI	YGGCCR	1 cut(s)	132
AcsI	RAATTY	1 cut(s)	17
AcuI	CTGAAG	2 cut(s)	178, 962
AcyI	GRCGYC	1 cut(s)	806
AdeI	CACNNNGTG	1 cut(s)	605
AfaI	GTAC	5 cut(s)	27, 71, 185, 374, 627
AfeI	AGCGCT	1 cut(s)	653
AfiI	CCNNNNNNNGG	3 cut(s)	168, 838, 1249
AflIII	ACRYGT	1 cut(s)	1308
AgsI	TTSAA	7 cut(s)	379, 647, 890, 961, 1100, 1423, 1615
AjiI	CACGTC	1 cut(s)	570
AjnI	CCWGG	1 cut(s)	273
AjuI	GAANNNNNNNTTGG	2 cut(s)	511, 543
AloI	GAACNNNNNNTCC	4 cut(s)	845, 877, 1295, 1327
Alw21I	GWGCWC	3 cut(s)	858, 1264, 1503
Alw26I	GTCTC	2 cut(s)	696, 797
AlwNI	CAGNNNCTG	2 cut(s)	367, 1670
Aor51HI	AGCGCT	1 cut(s)	653
AoxI	GGCC	5 cut(s)	132, 276, 752, 1160, 1239
ApeKI	GCWGC	7 cut(s)	234, 452, 578, 587, 1062, 1218, 1226
ApoI	RAATTY	1 cut(s)	17
AspLEI	GCGC	3 cut(s)	654, 760, 1675
AspS9I	GGNCC	3 cut(s)	1240, 1453, 1525
AsuHPI	GGTGA	1 cut(s)	451
AsuNHI	GCTAGC	1 cut(s)	1483
AvaII	GGWCC	2 cut(s)	1453, 1525
BanI	GGYRCC	2 cut(s)	34, 605
BanII	GRGCYC	1 cut(s)	858
BarI	GAAGNNNNNNTAC	2 cut(s)	176, 208
BauI	CACGAG	1 cut(s)	1257
BbsI	GAAGAC	1 cut(s)	234
Bbv12I	GWGCWC	3 cut(s)	858, 1264, 1503
BbvCI	CCTCAGC	1 cut(s)	899
BbvI	GCAGC	7 cut(s)	246, 439, 565, 574, 1074, 1205, 1213
BccI	CCATC	5 cut(s)	25, 331, 706, 827, 1516
BceAI	ACGGC	1 cut(s)	793
BciT130I	CCWGG	1 cut(s)	275
BciVI	GTATCC	1 cut(s)	1436
BcoDI	GTCTC	2 cut(s)	696, 797
BfaI	CTAG	4 cut(s)	750, 773, 1484, 1490
BfmI	CTRYAG	1 cut(s)	453
BfoI	RGCGCY	1 cut(s)	655
BfuI	GTATCC	1 cut(s)	1436
BmcAI	AGTACT	1 cut(s)	374
Bme1390I	CCNGG	1 cut(s)	275
Bme18I	GGWCC	2 cut(s)	1453, 1525
BmgBI	CACGTC	1 cut(s)	570
BmgT120I	GGNCC	3 cut(s)	1240, 1453, 1525
BmiI	GGNNCC	5 cut(s)	36, 607, 843, 1432, 1527
BmrFI	CCNGG	1 cut(s)	275
BmsI	GCATC	5 cut(s)	628, 1006, 1028, 1502, 1676
BmtI	GCTAGC	1 cut(s)	1487
BpiI	GAAGAC	1 cut(s)	234
BplI	GAGNNNNNCTC	2 cut(s)	1440, 1472
BpmI	CTGGAG	1 cut(s)	389
Bpu10I	CCTNAGC	1 cut(s)	899
BpuEI	CTTGAG	3 cut(s)	842, 1177, 1489
BsaHI	GRCGYC	1 cut(s)	806
BsaJI	CCNNGG	1 cut(s)	1057
BsaXI	ACNNNNNCTCC	6 cut(s)	356, 386, 845, 875, 897, 927
Bsc4I	CCNNNNNNNGG	3 cut(s)	168, 838, 1249
Bse1I	ACTGG	2 cut(s)	135, 768
Bse3DI	GCAATG	1 cut(s)	1206
BseBI	CCWGG	1 cut(s)	275
BseDI	CCNNGG	1 cut(s)	1057
BseGI	GGATG	1 cut(s)	643
BseLI	CCNNNNNNNGG	3 cut(s)	168, 838, 1249
BseMI	GCAATG	1 cut(s)	1206
BseMII	CTCAG	3 cut(s)	527, 890, 1454
BseNI	ACTGG	2 cut(s)	135, 768
BseRI	GAGGAG	1 cut(s)	1667
BseXI	GCAGC	7 cut(s)	246, 439, 565, 574, 1074, 1205, 1213
BseYI	CCCAGC	2 cut(s)	230, 1438
BsgI	GTGCAG	2 cut(s)	1204, 1331
Bsh1236I	CGCG	1 cut(s)	179
Bsh1285I	CGRYCG	1 cut(s)	1635
BshFI	GGCC	5 cut(s)	134, 278, 754, 1162, 1241
BshNI	GGYRCC	2 cut(s)	34, 605
BsiEI	CGRYCG	1 cut(s)	1635
BsiHKAI	GWGCWC	3 cut(s)	858, 1264, 1503
BsiSI	CCGG	3 cut(s)	38, 1365, 1699
BslI	CCNNNNNNNGG	3 cut(s)	168, 838, 1249
BsmAI	GTCTC	2 cut(s)	696, 797
BsmBI	CGTCTC	2 cut(s)	696, 797
BsnI	GGCC	5 cut(s)	134, 278, 754, 1162, 1241
Bsp1286I	GDGCHC	3 cut(s)	858, 1264, 1503
Bsp1407I	TGTACA	1 cut(s)	183
Bsp143I	GATC	2 cut(s)	495, 1632
BspANI	GGCC	5 cut(s)	134, 278, 754, 1162, 1241
BspCNI	CTCAG	3 cut(s)	526, 891, 1455
BspFNI	CGCG	1 cut(s)	179
BspLI	GGNNCC	5 cut(s)	36, 607, 843, 1432, 1527
BspMAI	CTGCAG	1 cut(s)	457
BspOI	GCTAGC	1 cut(s)	1487
BspQI	GCTCTTC	2 cut(s)	642, 938
BspT107I	GGYRCC	2 cut(s)	34, 605
BsrDI	GCAATG	1 cut(s)	1206
BsrGI	TGTACA	1 cut(s)	183
BsrI	ACTGG	2 cut(s)	135, 768
BssECI	CCNNGG	1 cut(s)	1057
BssMI	GATC	2 cut(s)	495, 1632
BssNI	GRCGYC	1 cut(s)	806
BssSI	CACGAG	1 cut(s)	1257
BssT1I	CCWWGG	1 cut(s)	1057
Bst2BI	CACGAG	1 cut(s)	1257
Bst2UI	CCWGG	1 cut(s)	275
Bst4CI	ACNGT	5 cut(s)	155, 605, 665, 779, 799
Bst6I	CTCTTC	3 cut(s)	642, 938, 1577
BstACI	GRCGYC	1 cut(s)	806
BstAPI	GCANNNNNTGC	1 cut(s)	587
BstAUI	TGTACA	1 cut(s)	183
BstC8I	GCNNGC	9 cut(s)	177, 215, 219, 595, 616, 620, 756, 1216, 1485
BstDEI	CTNAG	3 cut(s)	513, 899, 1463
BstEII	GGTNACC	1 cut(s)	1075
BstF5I	GGATG	1 cut(s)	643
BstFNI	CGCG	1 cut(s)	179
BstH2I	RGCGCY	1 cut(s)	655
BstHHI	GCGC	3 cut(s)	654, 760, 1675
BstKTI	GATC	2 cut(s)	498, 1635
BstMAI	GTCTC	2 cut(s)	696, 797
BstMBI	GATC	2 cut(s)	495, 1632
BstMCI	CGRYCG	1 cut(s)	1635
BstMWI	GCNNNNNNNGC	8 cut(s)	485, 584, 587, 638, 744, 853, 1215, 1484
BstNI	CCWGG	1 cut(s)	275
BstNSI	RCATGY	3 cut(s)	632, 1312, 1354
BstPI	GGTNACC	1 cut(s)	1075
BstSCI	CCNGG	1 cut(s)	273
BstSFI	CTRYAG	1 cut(s)	453
BstUI	CGCG	1 cut(s)	179
BstV1I	GCAGC	7 cut(s)	246, 439, 565, 574, 1074, 1205, 1213
BstV2I	GAAGAC	1 cut(s)	234
BstXI	CCANNNNNNTGG	1 cut(s)	1087
BsuI	GTATCC	1 cut(s)	1436
BsuRI	GGCC	5 cut(s)	134, 278, 754, 1162, 1241
BtrI	CACGTC	1 cut(s)	570
BtsCI	GGATG	1 cut(s)	643
BtsI	GCAGTG	2 cut(s)	1485, 1693
BtsIMutI	CAGTG	4 cut(s)	128, 761, 1485, 1693
Cac8I	GCNNGC	9 cut(s)	177, 215, 219, 595, 616, 620, 756, 1216, 1485
CaiI	CAGNNNCTG	2 cut(s)	367, 1670
CfoI	GCGC	3 cut(s)	654, 760, 1675
Cfr13I	GGNCC	3 cut(s)	1240, 1453, 1525
CseI	GACGC	2 cut(s)	485, 814
Csp6I	GTAC	5 cut(s)	26, 70, 184, 373, 626
CspCI	CAANNNNNGTGG	4 cut(s)	679, 714, 1557, 1592
CviAII	CATG	8 cut(s)	58, 629, 1033, 1039, 1300, 1309, 1351, 1516
CviQI	GTAC	5 cut(s)	26, 70, 184, 373, 626
DdeI	CTNAG	3 cut(s)	513, 899, 1463
DpnI	GATC	2 cut(s)	497, 1634
DpnII	GATC	2 cut(s)	495, 1632
DraIII	CACNNNGTG	1 cut(s)	605
EaeI	YGGCCR	1 cut(s)	132
Eam1104I	CTCTTC	3 cut(s)	642, 938, 1577
EarI	CTCTTC	3 cut(s)	642, 938, 1577
EciI	GGCGGA	1 cut(s)	359
Ecl136II	GAGCTC	1 cut(s)	856
Eco130I	CCWWGG	1 cut(s)	1057
Eco147I	AGGCCT	1 cut(s)	754
Eco24I	GRGCYC	1 cut(s)	858
Eco47I	GGWCC	2 cut(s)	1453, 1525
Eco47III	AGCGCT	1 cut(s)	653
Eco53kI	GAGCTC	1 cut(s)	856
Eco57I	CTGAAG	2 cut(s)	178, 962
Eco91I	GGTNACC	1 cut(s)	1075
EcoICRI	GAGCTC	1 cut(s)	856
EcoO109I	RGGNCCY	1 cut(s)	1453
EcoO65I	GGTNACC	1 cut(s)	1075
EcoRII	CCWGG	1 cut(s)	273
EcoT14I	CCWWGG	1 cut(s)	1057
EcoT22I	ATGCAT	1 cut(s)	1021
EcoT38I	GRGCYC	1 cut(s)	858
ErhI	CCWWGG	1 cut(s)	1057
Esp3I	CGTCTC	2 cut(s)	696, 797
FaeI	CATG	8 cut(s)	61, 632, 1036, 1042, 1303, 1312, 1354, 1519
FatI	CATG	8 cut(s)	57, 628, 1032, 1038, 1299, 1308, 1350, 1515
FauI	CCCGC	1 cut(s)	615
FblI	GTMKAC	2 cut(s)	261, 572
FokI	GGATG	1 cut(s)	650
FriOI	GRGCYC	1 cut(s)	858
FspBI	CTAG	4 cut(s)	750, 773, 1484, 1490
GlaI	GCGC	3 cut(s)	653, 759, 1674
GsaI	CCCAGC	2 cut(s)	234, 1442
GsuI	CTGGAG	1 cut(s)	389
HaeII	RGCGCY	1 cut(s)	655
HaeIII	GGCC	5 cut(s)	134, 278, 754, 1162, 1241
HapII	CCGG	3 cut(s)	38, 1365, 1699
HgaI	GACGC	2 cut(s)	485, 814
HhaI	GCGC	3 cut(s)	654, 760, 1675
Hin1I	GRCGYC	1 cut(s)	806
Hin1II	CATG	8 cut(s)	61, 632, 1036, 1042, 1303, 1312, 1354, 1519
Hin6I	GCGC	3 cut(s)	652, 758, 1673
HinP1I	GCGC	3 cut(s)	652, 758, 1673
HincII	GTYRAC	4 cut(s)	262, 298, 409, 433
HindII	GTYRAC	4 cut(s)	262, 298, 409, 433
HindIII	AAGCTT	1 cut(s)	929
HinfI	GANTC	6 cut(s)	125, 308, 544, 790, 1335, 1395
HpaII	CCGG	3 cut(s)	38, 1365, 1699
HphI	GGTGA	1 cut(s)	451
Hpy166II	GTNNAC	6 cut(s)	262, 289, 298, 409, 433, 573
Hpy188III	TCNNGA	8 cut(s)	268, 362, 379, 976, 1011, 1123, 1156, 1511
Hpy8I	GTNNAC	6 cut(s)	262, 289, 298, 409, 433, 573
Hpy99I	CGWCG	2 cut(s)	331, 1073
HpyAV	CCTTC	8 cut(s)	7, 202, 344, 421, 439, 1165, 1469, 1621
HpyCH4III	ACNGT	5 cut(s)	155, 605, 665, 779, 799
HpyCH4IV	ACGT	5 cut(s)	329, 569, 705, 837, 965
HpyCH4V	TGCA	6 cut(s)	53, 455, 581, 1019, 1221, 1348
HpyF10VI	GCNNNNNNNGC	8 cut(s)	485, 584, 587, 638, 744, 853, 1215, 1484
HpyF3I	CTNAG	3 cut(s)	513, 899, 1463
HpySE526I	ACGT	5 cut(s)	329, 569, 705, 837, 965
Hsp92I	GRCGYC	1 cut(s)	806
Hsp92II	CATG	8 cut(s)	61, 632, 1036, 1042, 1303, 1312, 1354, 1519
HspAI	GCGC	3 cut(s)	652, 758, 1673
Kzo9I	GATC	2 cut(s)	495, 1632
LguI	GCTCTTC	2 cut(s)	642, 938
LmnI	GCTCC	7 cut(s)	364, 847, 853, 905, 1070, 1584, 1680
Lsp1109I	GCAGC	7 cut(s)	246, 439, 565, 574, 1074, 1205, 1213
LweI	GCATC	5 cut(s)	628, 1006, 1028, 1502, 1676
MaeI	CTAG	4 cut(s)	750, 773, 1484, 1490
MaeII	ACGT	5 cut(s)	329, 569, 705, 837, 965
MaeIII	GTNAC	4 cut(s)	781, 1075, 1252, 1689
MalI	GATC	2 cut(s)	497, 1634
MboI	GATC	2 cut(s)	495, 1632
MhlI	GDGCHC	3 cut(s)	858, 1264, 1503
MluCI	AATT	2 cut(s)	17, 1399
MlyI	GAGTC	3 cut(s)	302, 538, 1404
MmeI	TCCRAC	3 cut(s)	121, 711, 1092
Mph1103I	ATGCAT	1 cut(s)	1021
MseI	TTAA	4 cut(s)	74, 86, 399, 873
MslI	CAYNNNNRTG	3 cut(s)	1037, 1355, 1520
MspA1I	CMGCKG	4 cut(s)	198, 578, 898, 1226
MspI	CCGG	3 cut(s)	38, 1365, 1699
MspR9I	CCNGG	1 cut(s)	275
MvaI	CCWGG	1 cut(s)	275
MvnI	CGCG	1 cut(s)	179
MwoI	GCNNNNNNNGC	8 cut(s)	485, 584, 587, 638, 744, 853, 1215, 1484
NdeII	GATC	2 cut(s)	495, 1632
NheI	GCTAGC	1 cut(s)	1483
NlaIII	CATG	8 cut(s)	61, 632, 1036, 1042, 1303, 1312, 1354, 1519
NlaIV	GGNNCC	5 cut(s)	36, 607, 843, 1432, 1527
NmuCI	GTSAC	3 cut(s)	781, 1252, 1689
NsiI	ATGCAT	1 cut(s)	1021
NspI	RCATGY	3 cut(s)	632, 1312, 1354
PceI	AGGCCT	1 cut(s)	754
PciI	ACATGT	1 cut(s)	1308
PciSI	GCTCTTC	2 cut(s)	642, 938
PfeI	GAWTC	3 cut(s)	125, 790, 1335
Ple19I	CGATCG	1 cut(s)	1635
PleI	GAGTC	3 cut(s)	302, 538, 1403
PpsI	GAGTC	3 cut(s)	302, 538, 1403
PpuMI	RGGWCCY	1 cut(s)	1453
PscI	ACATGT	1 cut(s)	1308
Psp124BI	GAGCTC	1 cut(s)	858
Psp5II	RGGWCCY	1 cut(s)	1453
Psp6I	CCWGG	1 cut(s)	273
PspEI	GGTNACC	1 cut(s)	1075
PspFI	CCCAGC	2 cut(s)	230, 1438
PspGI	CCWGG	1 cut(s)	273
PspN4I	GGNNCC	5 cut(s)	36, 607, 843, 1432, 1527
PspPI	GGNCC	3 cut(s)	1240, 1453, 1525
PspPPI	RGGWCCY	1 cut(s)	1453
PstI	CTGCAG	1 cut(s)	457
PstNI	CAGNNNCTG	2 cut(s)	367, 1670
PvuI	CGATCG	1 cut(s)	1635
PvuII	CAGCTG	2 cut(s)	898, 1226
RsaI	GTAC	5 cut(s)	27, 71, 185, 374, 627
RsaNI	GTAC	5 cut(s)	26, 70, 184, 373, 626
RseI	CAYNNNNRTG	3 cut(s)	1037, 1355, 1520
SacI	GAGCTC	1 cut(s)	858
SalI	GTCGAC	1 cut(s)	260
SapI	GCTCTTC	2 cut(s)	642, 938
SaqAI	TTAA	4 cut(s)	74, 86, 399, 873
Sau3AI	GATC	2 cut(s)	495, 1632
Sau96I	GGNCC	3 cut(s)	1240, 1453, 1525
ScaI	AGTACT	1 cut(s)	374
SchI	GAGTC	3 cut(s)	302, 538, 1404
ScrFI	CCNGG	1 cut(s)	275
SduI	GDGCHC	3 cut(s)	858, 1264, 1503
SfaNI	GCATC	5 cut(s)	628, 1006, 1028, 1502, 1676
SfcI	CTRYAG	1 cut(s)	453
SinI	GGWCC	2 cut(s)	1453, 1525
SmiMI	CAYNNNNRTG	3 cut(s)	1037, 1355, 1520
SmlI	CTYRAG	3 cut(s)	857, 1156, 1504
SmoI	CTYRAG	3 cut(s)	857, 1156, 1504
Sse9I	AATT	2 cut(s)	17, 1399
SseBI	AGGCCT	1 cut(s)	754
SspMI	CTAG	4 cut(s)	750, 773, 1484, 1490
SstI	GAGCTC	1 cut(s)	858
StuI	AGGCCT	1 cut(s)	754
StyD4I	CCNGG	1 cut(s)	273
StyI	CCWWGG	1 cut(s)	1057
TaaI	ACNGT	5 cut(s)	155, 605, 665, 779, 799
TaiI	ACGT	5 cut(s)	332, 572, 708, 840, 968
TasI	AATT	2 cut(s)	17, 1399
TatI	WGTACW	2 cut(s)	183, 372
TauI	GCSGC	5 cut(s)	137, 140, 344, 593, 1387
TfiI	GAWTC	3 cut(s)	125, 790, 1335
Tru1I	TTAA	4 cut(s)	74, 86, 399, 873
Tru9I	TTAA	4 cut(s)	74, 86, 399, 873
TscAI	CASTG	4 cut(s)	135, 768, 1485, 1700
TseFI	GTSAC	3 cut(s)	781, 1252, 1689
TseI	GCWGC	7 cut(s)	234, 452, 578, 587, 1062, 1218, 1226
Tsp45I	GTSAC	3 cut(s)	781, 1252, 1689
TspGWI	ACGGA	2 cut(s)	866, 966
TspRI	CASTG	4 cut(s)	135, 768, 1485, 1700
VpaK11BI	GGWCC	2 cut(s)	1453, 1525
XapI	RAATTY	1 cut(s)	17
XceI	RCATGY	3 cut(s)	632, 1312, 1354
XmiI	GTMKAC	2 cut(s)	261, 572
XspI	CTAG	4 cut(s)	750, 773, 1484, 1490
ZrmI	AGTACT	1 cut(s)	374
Zsp2I	ATGCAT	1 cut(s)	1021

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.