pycom16g06460

Belongs to the cullin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0003908
Physical Location & Seq
Reverse (-)
4142254 .. 4143772
1519 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1020 bp
ATGAGTAACCAGAAGAAGAGGAATTTTCAGATAGAGGCGTTCAAGCACAGGGTGGTGGTGGATCCGAAATACGCAGACAAGACGTGGAAGGTTCTGGAGCACGCAATCCGCGAGATTTACAATCACAATGCCAGTGGCCTCAGCTTCGAAGAGCTTTACAGAAATGCCTACAATATGGTGCTCCACAAATTCGGCGAGAAGCTTTACTCGGGACTGGTTACAACCATGACTTCTCATCTCAAAGAGATATCTAAATCAATTGAATCTGCTCAGGGTGGAATGTTTCTTGAAGAAATGAATAAGAAATGGACTGAACACAACAAGGCGTTGCAGATGATTCGAGACATTCTGATGTACATGGACAGGACTTACATTCCGAGCACTCAGAAAACCCCAGTTCACGAGCTTGGGCTCAACCTTTGGAGAGACAATATTGTCCGCTCTAACAAGATTCAGACGAGGCTTCTTAACACACTTATTGAACTAGTGCTCAGAGAACGAACCGGTAATGTTATCAACCGGGGTTTGATGAGGAGTATAATTAAGATGCTTATGGATTTAGGTCCTTCAGTTTACCGCGAGGACTTTGAGAATCATTTTCTTGAGGTTTCAGCTGAATTTTACAAGGGCGAGTCTCAAAAATTTATCGAGTGCTGCGATTGTGGGGATTATCTGCAGCAGGCTGAGAGGCGCCTGAATGAGGAAGTGGAAAGGGTGACACATTACTTGGATGCCAAGAGTGAAGCCAAGATAACGAATGTGGTAGAGGAGGAGGTGATTGCAAACCACATGCTGAGATTAGTCCACATGGAGAATTCAGGTTTGGTGAACATGCTTTTCGATGATAAATATGAAGACCTTGGAAGAATGTACAGCTTGTTCCGTCGGGTTGCCAACGGTCTCTCAACAATACGAGAAGTGATGACTTCTCACATCAGGGGAACTGGTAAACAGCTGGTTACCGATCGATCCAGAAAGGTTGAAGGATCCAGTGGAGTTTGTCCAGAGGCTCTTGGATGA

Protein Analysis

340

Amino Acids

39.45

Weight (kDa)

7.72

Isoelectric Point (pI)

47.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin PF00888 29 - 324 4.1e-92 Cullin alpha solenoid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 434
AccB1I GGYRCC 1 cut(s) 690
AccBSI CCGCTC 1 cut(s) 441
AccII CGCG 2 cut(s) 111, 579
AciI CCGC 3 cut(s) 109, 439, 577
AclWI GGATC 5 cut(s) 56, 69, 963, 981, 994
AcsI RAATTY 5 cut(s) 22, 188, 617, 641, 814
AcuI CTGAAG 1 cut(s) 552
AcyI GRCGYC 1 cut(s) 691
AdeI CACNNNGTG 1 cut(s) 52
AfaI GTAC 2 cut(s) 356, 872
AfiI CCNNNNNNNGG 1 cut(s) 700
AgeI ACCGGT 1 cut(s) 503
AgsI TTSAA 5 cut(s) 43, 263, 290, 482, 983
AhlI ACTAGT 1 cut(s) 484
AjiI CACGTC 1 cut(s) 84
AjuI GAANNNNNNNTTGG 2 cut(s) 806, 838
AluBI AGCT 7 cut(s) 144, 154, 202, 406, 614, 876, 955
AluI AGCT 7 cut(s) 144, 154, 202, 406, 614, 876, 955
Alw21I GWGCWC 4 cut(s) 102, 183, 383, 492
Alw26I GTCTC 4 cut(s) 336, 420, 639, 905
AlwI GGATC 5 cut(s) 56, 69, 963, 981, 994
Ama87I CYCGRG 1 cut(s) 208
AoxI GGCC 1 cut(s) 136
ApeKI GCWGC 2 cut(s) 654, 676
ApoI RAATTY 5 cut(s) 22, 188, 617, 641, 814
AsiGI ACCGGT 1 cut(s) 503
AspLEI GCGC 1 cut(s) 693
AspS9I GGNCC 1 cut(s) 563
AsuC2I CCSGG 1 cut(s) 521
AsuHPI GGTGA 3 cut(s) 727, 787, 838
AsuII TTCGAA 1 cut(s) 147
AvaI CYCGRG 1 cut(s) 208
AvaII GGWCC 1 cut(s) 563
BamHI GGATCC 2 cut(s) 61, 986
BanI GGYRCC 1 cut(s) 690
BanII GRGCYC 1 cut(s) 414
BauI CACGAG 1 cut(s) 401
BbsI GAAGAC 1 cut(s) 861
Bbv12I GWGCWC 4 cut(s) 102, 183, 383, 492
BbvCI CCTCAGC 1 cut(s) 140
BbvI GCAGC 2 cut(s) 641, 688
BcgI CGANNNNNNTGC 2 cut(s) 320, 354
BcnI CCSGG 1 cut(s) 521
BcoDI GTCTC 4 cut(s) 336, 420, 639, 905
BcuI ACTAGT 1 cut(s) 484
BfaI CTAG 1 cut(s) 485
BfmI CTRYAG 1 cut(s) 674
BfoI RGCGCY 1 cut(s) 694
BisI GCNGC 2 cut(s) 655, 677
BlsI GCNGC 2 cut(s) 656, 678
Bme1390I CCNGG 1 cut(s) 521
Bme18I GGWCC 1 cut(s) 563
BmeT110I CYCGRG 1 cut(s) 208
BmgBI CACGTC 1 cut(s) 84
BmgT120I GGNCC 1 cut(s) 563
BmiI GGNNCC 3 cut(s) 63, 692, 988
BmrFI CCNGG 1 cut(s) 521
BmrI ACTGGG 1 cut(s) 389
BmsI GCATC 2 cut(s) 537, 721
BmuI ACTGGG 1 cut(s) 389
BpiI GAAGAC 1 cut(s) 861
BpmI CTGGAG 1 cut(s) 116
Bpu10I CCTNAGC 2 cut(s) 140, 270
Bpu14I TTCGAA 1 cut(s) 147
BpuEI CTTGAG 1 cut(s) 623
BpuMI CCSGG 1 cut(s) 521
Bsa29I ATCGAT 1 cut(s) 967
BsaHI GRCGYC 1 cut(s) 691
BsaI GGTCTC 1 cut(s) 905
BsaJI CCNNGG 2 cut(s) 520, 859
BsaWI WCCGGW 1 cut(s) 503
Bsc4I CCNNNNNNNGG 1 cut(s) 700
Bse118I RCCGGY 1 cut(s) 503
Bse1I ACTGG 5 cut(s) 132, 219, 395, 949, 990
BseCI ATCGAT 1 cut(s) 967
BseDI CCNNGG 2 cut(s) 520, 859
BseGI GGATG 1 cut(s) 736
BseLI CCNNNNNNNGG 1 cut(s) 700
BseMII CTCAG 6 cut(s) 154, 284, 398, 505, 675, 785
BseNI ACTGG 5 cut(s) 132, 219, 395, 949, 990
BseRI GAGGAG 3 cut(s) 547, 782, 785
BseXI GCAGC 2 cut(s) 641, 688
Bsh1236I CGCG 2 cut(s) 111, 579
Bsh1285I CGRYCG 1 cut(s) 967
BshFI GGCC 1 cut(s) 138
BshNI GGYRCC 1 cut(s) 690
BshTI ACCGGT 1 cut(s) 503
BshVI ATCGAT 1 cut(s) 967
BsiEI CGRYCG 1 cut(s) 967
BsiHKAI GWGCWC 4 cut(s) 102, 183, 383, 492
BsiHKCI CYCGRG 1 cut(s) 208
BsiSI CCGG 2 cut(s) 504, 520
BslFI GGGAC 1 cut(s) 225
BslI CCNNNNNNNGG 1 cut(s) 700
BsmAI GTCTC 4 cut(s) 336, 420, 639, 905
BsmFI GGGAC 1 cut(s) 225
BsnI GGCC 1 cut(s) 138
Bso31I GGTCTC 1 cut(s) 905
BsoBI CYCGRG 1 cut(s) 208
Bsp119I TTCGAA 1 cut(s) 147
Bsp1286I GDGCHC 5 cut(s) 102, 183, 383, 414, 492
Bsp1407I TGTACA 2 cut(s) 354, 870
Bsp143I GATC 4 cut(s) 61, 964, 968, 986
BspACI CCGC 3 cut(s) 109, 439, 577
BspANI GGCC 1 cut(s) 138
BspCNI CTCAG 6 cut(s) 153, 283, 397, 504, 676, 786
BspDI ATCGAT 1 cut(s) 967
BspFNI CGCG 2 cut(s) 111, 579
BspLI GGNNCC 3 cut(s) 63, 692, 988
BspMAI CTGCAG 1 cut(s) 678
BspPI GGATC 5 cut(s) 56, 69, 963, 981, 994
BspQI GCTCTTC 1 cut(s) 144
BspT104I TTCGAA 1 cut(s) 147
BspT107I GGYRCC 1 cut(s) 690
BspTNI GGTCTC 1 cut(s) 905
BsrBI CCGCTC 1 cut(s) 441
BsrFI RCCGGY 1 cut(s) 503
BsrGI TGTACA 2 cut(s) 354, 870
BsrI ACTGG 5 cut(s) 132, 219, 395, 949, 990
BssAI RCCGGY 1 cut(s) 503
BssECI CCNNGG 2 cut(s) 520, 859
BssMI GATC 4 cut(s) 61, 964, 968, 986
BssNI GRCGYC 1 cut(s) 691
BssSI CACGAG 1 cut(s) 401
BssT1I CCWWGG 1 cut(s) 859
Bst2BI CACGAG 1 cut(s) 401
Bst4CI ACNGT 1 cut(s) 899
Bst6I CTCTTC 2 cut(s) 11, 144
BstACI GRCGYC 1 cut(s) 691
BstAUI TGTACA 2 cut(s) 354, 870
BstBI TTCGAA 1 cut(s) 147
BstC8I GCNNGC 2 cut(s) 102, 681
BstDEI CTNAG 6 cut(s) 140, 270, 384, 491, 684, 794
BstEII GGTNACC 1 cut(s) 958
BstENI CCTNNNNNAGG 1 cut(s) 698
BstF5I GGATG 1 cut(s) 736
BstFNI CGCG 2 cut(s) 111, 579
BstH2I RGCGCY 1 cut(s) 694
BstHHI GCGC 1 cut(s) 693
BstKTI GATC 4 cut(s) 64, 967, 971, 989
BstMAI GTCTC 4 cut(s) 336, 420, 639, 905
BstMBI GATC 4 cut(s) 61, 964, 968, 986
BstMCI CGRYCG 1 cut(s) 967
BstNSI RCATGY 2 cut(s) 793, 835
BstPI GGTNACC 1 cut(s) 958
BstSCI CCNGG 1 cut(s) 519
BstSFI CTRYAG 1 cut(s) 674
BstUI CGCG 2 cut(s) 111, 579
BstV1I GCAGC 2 cut(s) 641, 688
BstV2I GAAGAC 1 cut(s) 861
BstX2I RGATCY 2 cut(s) 61, 986
BstYI RGATCY 2 cut(s) 61, 986
Bsu15I ATCGAT 1 cut(s) 967
BsuRI GGCC 1 cut(s) 138
BsuTUI ATCGAT 1 cut(s) 967
BtrI CACGTC 1 cut(s) 84
BtsCI GGATG 1 cut(s) 736
BtsIMutI CAGTG 2 cut(s) 139, 997
Cac8I GCNNGC 2 cut(s) 102, 681
CfoI GCGC 1 cut(s) 693
Cfr10I RCCGGY 1 cut(s) 503
Cfr13I GGNCC 1 cut(s) 563
ClaI ATCGAT 1 cut(s) 967
Csp6I GTAC 2 cut(s) 355, 871
CspAI ACCGGT 1 cut(s) 503
CspCI CAANNNNNGTGG 2 cut(s) 115, 150
CviAII CATG 5 cut(s) 226, 358, 790, 808, 832
CviQI GTAC 2 cut(s) 355, 871
DdeI CTNAG 6 cut(s) 140, 270, 384, 491, 684, 794
DinI GGCGCC 1 cut(s) 692
DpnI GATC 4 cut(s) 63, 966, 970, 988
DpnII GATC 4 cut(s) 61, 964, 968, 986
DraIII CACNNNGTG 1 cut(s) 52
DrdI GACNNNNNNGTC 1 cut(s) 434
DseDI GACNNNNNNGTC 1 cut(s) 434
Eam1104I CTCTTC 2 cut(s) 11, 144
EarI CTCTTC 2 cut(s) 11, 144
Eco130I CCWWGG 1 cut(s) 859
Eco24I GRGCYC 1 cut(s) 414
Eco31I GGTCTC 1 cut(s) 905
Eco32I GATATC 1 cut(s) 249
Eco47I GGWCC 1 cut(s) 563
Eco57I CTGAAG 1 cut(s) 552
Eco88I CYCGRG 1 cut(s) 208
Eco91I GGTNACC 1 cut(s) 958
EcoNI CCTNNNNNAGG 1 cut(s) 698
EcoO109I RGGNCCY 1 cut(s) 563
EcoO65I GGTNACC 1 cut(s) 958
EcoRI GAATTC 1 cut(s) 814
EcoRV GATATC 1 cut(s) 249
EcoT14I CCWWGG 1 cut(s) 859
EcoT38I GRGCYC 1 cut(s) 414
EgeI GGCGCC 1 cut(s) 692
EheI GGCGCC 1 cut(s) 692
ErhI CCWWGG 1 cut(s) 859
FaeI CATG 5 cut(s) 229, 361, 793, 811, 835
FaiI YATR 9 cut(s) 176, 227, 359, 539, 554, 791, 809, 833, 852
FaqI GGGAC 1 cut(s) 225
FatI CATG 5 cut(s) 225, 357, 789, 807, 831
Fnu4HI GCNGC 2 cut(s) 655, 677
FokI GGATG 1 cut(s) 743
FriOI GRGCYC 1 cut(s) 414
Fsp4HI GCNGC 2 cut(s) 655, 677
FspBI CTAG 1 cut(s) 485
GlaI GCGC 1 cut(s) 692
GluI GCNGC 2 cut(s) 655, 677
GsuI CTGGAG 1 cut(s) 116
HaeII RGCGCY 1 cut(s) 694
HaeIII GGCC 1 cut(s) 138
HapII CCGG 2 cut(s) 504, 520
HhaI GCGC 1 cut(s) 693
Hin1I GRCGYC 1 cut(s) 691
Hin1II CATG 5 cut(s) 229, 361, 793, 811, 835
Hin6I GCGC 1 cut(s) 691
HinP1I GCGC 1 cut(s) 691
HindIII AAGCTT 1 cut(s) 200
HinfI GANTC 5 cut(s) 263, 337, 451, 592, 632
HpaII CCGG 2 cut(s) 504, 520
HphI GGTGA 3 cut(s) 727, 787, 838
Hpy166II GTNNAC 5 cut(s) 400, 574, 805, 829, 950
Hpy188I TCNGA 7 cut(s) 30, 66, 351, 378, 387, 456, 494
Hpy188III TCNNGA 8 cut(s) 95, 210, 287, 341, 401, 602, 972, 1004
Hpy8I GTNNAC 5 cut(s) 400, 574, 805, 829, 950
Hpy99I CGWCG 1 cut(s) 888
HpyAV CCTTC 3 cut(s) 82, 576, 977
HpyCH4III ACNGT 1 cut(s) 899
HpyCH4IV ACGT 1 cut(s) 83
HpyCH4V TGCA 3 cut(s) 331, 676, 782
HpyF3I CTNAG 6 cut(s) 140, 270, 384, 491, 684, 794
HpySE526I ACGT 1 cut(s) 83
Hsp92I GRCGYC 1 cut(s) 691
Hsp92II CATG 5 cut(s) 229, 361, 793, 811, 835
HspAI GCGC 1 cut(s) 691
KasI GGCGCC 1 cut(s) 690
Kzo9I GATC 4 cut(s) 61, 964, 968, 986
LguI GCTCTTC 1 cut(s) 144
LmnI GCTCC 2 cut(s) 97, 186
Lsp1109I GCAGC 2 cut(s) 641, 688
LweI GCATC 2 cut(s) 537, 721
MaeI CTAG 1 cut(s) 485
MaeII ACGT 1 cut(s) 83
MaeIII GTNAC 4 cut(s) 5, 217, 715, 958
MalI GATC 4 cut(s) 63, 966, 970, 988
MbiI CCGCTC 1 cut(s) 441
MboI GATC 4 cut(s) 61, 964, 968, 986
MboII GAAGA 6 cut(s) 25, 28, 161, 302, 866, 876
MfeI CAATTG 1 cut(s) 258
MflI RGATCY 2 cut(s) 61, 986
MhlI GDGCHC 5 cut(s) 102, 183, 383, 414, 492
MluCI AATT 7 cut(s) 22, 188, 258, 540, 617, 641, 814
Mly113I GGCGCC 1 cut(s) 691
MlyI GAGTC 1 cut(s) 641
MseI TTAA 2 cut(s) 468, 543
MslI CAYNNNNRTG 1 cut(s) 350
MspA1I CMGCKG 2 cut(s) 614, 955
MspI CCGG 2 cut(s) 504, 520
MspR9I CCNGG 1 cut(s) 521
MunI CAATTG 1 cut(s) 258
MvnI CGCG 2 cut(s) 111, 579
NarI GGCGCC 1 cut(s) 691
NciI CCSGG 1 cut(s) 521
NdeII GATC 4 cut(s) 61, 964, 968, 986
NlaIII CATG 5 cut(s) 229, 361, 793, 811, 835
NlaIV GGNNCC 3 cut(s) 63, 692, 988
NmuCI GTSAC 1 cut(s) 715
NspI RCATGY 2 cut(s) 793, 835
NspV TTCGAA 1 cut(s) 147
PciSI GCTCTTC 1 cut(s) 144
PcsI WCGNNNNNNNCGW 2 cut(s) 108, 654
PfeI GAWTC 4 cut(s) 263, 337, 451, 592
PinAI ACCGGT 1 cut(s) 503
PkrI GCNGC 2 cut(s) 656, 678
Ple19I CGATCG 1 cut(s) 967
PleI GAGTC 1 cut(s) 640
PluTI GGCGCC 1 cut(s) 694
PpsI GAGTC 1 cut(s) 640
PpuMI RGGWCCY 1 cut(s) 563
Psp5II RGGWCCY 1 cut(s) 563
PspEI GGTNACC 1 cut(s) 958
PspN4I GGNNCC 3 cut(s) 63, 692, 988
PspPI GGNCC 1 cut(s) 563
PspPPI RGGWCCY 1 cut(s) 563
PsrI GAACNNNNNNTAC 2 cut(s) 863, 895
PstI CTGCAG 1 cut(s) 678
PsuI RGATCY 2 cut(s) 61, 986
PvuI CGATCG 1 cut(s) 967
PvuII CAGCTG 2 cut(s) 614, 955
RsaI GTAC 2 cut(s) 356, 872
RsaNI GTAC 2 cut(s) 355, 871
RseI CAYNNNNRTG 1 cut(s) 350
SapI GCTCTTC 1 cut(s) 144
SaqAI TTAA 2 cut(s) 468, 543
SatI GCNGC 2 cut(s) 655, 677
Sau3AI GATC 4 cut(s) 61, 964, 968, 986
Sau96I GGNCC 1 cut(s) 563
SchI GAGTC 1 cut(s) 641
ScrFI CCNGG 1 cut(s) 521
SduI GDGCHC 5 cut(s) 102, 183, 383, 414, 492
SfaNI GCATC 2 cut(s) 537, 721
SfcI CTRYAG 1 cut(s) 674
SfoI GGCGCC 1 cut(s) 692
SfuI TTCGAA 1 cut(s) 147
SinI GGWCC 1 cut(s) 563
SmiMI CAYNNNNRTG 1 cut(s) 350
SmlI CTYRAG 1 cut(s) 602
SmoI CTYRAG 1 cut(s) 602
SpeI ACTAGT 1 cut(s) 484
Sse9I AATT 7 cut(s) 22, 188, 258, 540, 617, 641, 814
SsiI CCGC 3 cut(s) 109, 439, 577
SspDI GGCGCC 1 cut(s) 690
SspI AATATT 1 cut(s) 433
SspMI CTAG 1 cut(s) 485
StyD4I CCNGG 1 cut(s) 519
StyI CCWWGG 1 cut(s) 859
TaaI ACNGT 1 cut(s) 899
TaiI ACGT 1 cut(s) 86
TaqI TCGA 5 cut(s) 147, 340, 648, 840, 967
TasI AATT 7 cut(s) 22, 188, 258, 540, 617, 641, 814
TatI WGTACW 2 cut(s) 354, 870
TfiI GAWTC 4 cut(s) 263, 337, 451, 592
Tru1I TTAA 2 cut(s) 468, 543
Tru9I TTAA 2 cut(s) 468, 543
TscAI CASTG 2 cut(s) 139, 997
TseFI GTSAC 1 cut(s) 715
TseI GCWGC 2 cut(s) 654, 676
Tsp45I GTSAC 1 cut(s) 715
TspDTI ATGAA 2 cut(s) 311, 867
TspGWI ACGGA 1 cut(s) 872
TspRI CASTG 2 cut(s) 139, 997
VpaK11BI GGWCC 1 cut(s) 563
XagI CCTNNNNNAGG 1 cut(s) 698
XapI RAATTY 5 cut(s) 22, 188, 617, 641, 814
XceI RCATGY 2 cut(s) 793, 835
XspI CTAG 1 cut(s) 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.