pycom16g07190

serine threonine-protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0003085
Physical Location & Seq
Forward (+)
4688525 .. 4690961
2437 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 921 bp
ATGCCGTCTCATTCGGATCTGGACCGTCAGATCGAGCATCTGATGGAGTGCAAGACGTTGCCGGAGGCGGAGGTGAAGACGCTGTGCGAGCAGGCGAGGCCGATCCTGGTGGAGGAGTGGAACGTGCAGCCGGTGAAGTGCCCCGTGACGGTGTGCGGAGATATACACGGCCAGTTTTACGACCTCATTGAGCTGTTTCGGATAGGAGGGAACGCTCCCGATACTAATTACTGTTTTATGGGTGATTATGTAGATCGTGGGTACTATTCCGTGGAGACTGTCACGCTTCTGGTCGCCCTCAAAGTCCGTTATAGAGATAGAATTACAATCCTCAGAGGAAATCATGAGAGTCGGCAAATTACTCAAGTGTATGGTTTTTATGACGAATGCTTGAGAAAGTATGGGAATGCCAATGTCTGGAAGTTCTTTACCGATTTATTTGATTATCTTCCCCTTACAGCCCTCATTGAGAGTCAGGTCTTCTGTTTGCACGGGGGTCTTTCACCATCTTTGGACACTTTGGACAATATTCGAGCTTTGGACCGTATACAGGAGGTTCCACATGAAGGACCAATGTGCGATCTCTTGTGGTCTGACCCAGATGACCGCTGTGGGTGGGGAATATCTCCACGTGGTGCTGGTTATACATTCGGGCAGGATATAGCCGCTCAGTTTAACCATACCAATGGACTCAGTCTGATTTCAAGAGCCCATCAGCTTGTCATGGAAGGATACAATTGGTGCCAGGAGAAGAATGTGGTGACTGTTTTTAGCGCTCCAAACTATTGTTATCGGTGCGGGAACATGGCTGCAATTTTGGAAATTGGGGAGAACATGGAACAGAATTTTCTGCAGTTTGATCCCGCCCCTCGTCAAATTGAACCTGACAACACACGCAAGACTCCGGACTATTTTTTGTAA

Protein Analysis

307

Amino Acids

35.08

Weight (kDa)

4.86

Isoelectric Point (pI)

40.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 48 - 241 6.2e-45 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 741
AccB7I CCANNNNNTGG 1 cut(s) 417
AccBSI CCGCTC 1 cut(s) 668
AccI GTMKAC 1 cut(s) 547
AccIII TCCGGA 1 cut(s) 904
AciI CCGC 6 cut(s) 68, 156, 607, 666, 798, 864
AclWI GGATC 3 cut(s) 24, 97, 854
AcoI YGGCCR 1 cut(s) 169
AcsI RAATTY 1 cut(s) 844
AcvI CACGTG 1 cut(s) 632
AdeI CACNNNGTG 1 cut(s) 635
AfaI GTAC 1 cut(s) 263
AfeI AGCGCT 1 cut(s) 775
AfiI CCNNNNNNNGG 5 cut(s) 112, 148, 417, 550, 566
AgsI TTSAA 2 cut(s) 705, 881
AjnI CCWGG 2 cut(s) 105, 744
AluBI AGCT 3 cut(s) 193, 536, 718
AluI AGCT 3 cut(s) 193, 536, 718
Alw26I GTCTC 2 cut(s) 12, 269
AlwI GGATC 3 cut(s) 24, 97, 854
Aor13HI TCCGGA 1 cut(s) 904
Aor51HI AGCGCT 1 cut(s) 775
AoxI GGCC 2 cut(s) 98, 169
ApeKI GCWGC 2 cut(s) 127, 809
ApoI RAATTY 1 cut(s) 844
AspLEI GCGC 1 cut(s) 776
AspS9I GGNCC 3 cut(s) 22, 541, 569
AsuHPI GGTGA 5 cut(s) 85, 145, 254, 495, 772
AvaII GGWCC 3 cut(s) 22, 541, 569
BaeGI GKGCMC 1 cut(s) 143
BanI GGYRCC 1 cut(s) 741
BanII GRGCYC 1 cut(s) 712
BbrPI CACGTG 1 cut(s) 632
BbsI GAAGAC 2 cut(s) 83, 472
BbvI GCAGC 2 cut(s) 139, 796
BccI CCATC 3 cut(s) 37, 514, 720
BceAI ACGGC 1 cut(s) 184
BciT130I CCWGG 2 cut(s) 107, 746
BciVI GTATCC 1 cut(s) 725
BcoDI GTCTC 2 cut(s) 12, 269
BfmI CTRYAG 1 cut(s) 851
BfoI RGCGCY 1 cut(s) 777
BfuI GTATCC 1 cut(s) 725
BisI GCNGC 3 cut(s) 128, 666, 810
BlsI GCNGC 3 cut(s) 129, 667, 811
Bme1390I CCNGG 2 cut(s) 107, 746
Bme18I GGWCC 3 cut(s) 22, 541, 569
BmgT120I GGNCC 3 cut(s) 22, 541, 569
BmiI GGNNCC 2 cut(s) 558, 743
BmrFI CCNGG 2 cut(s) 107, 746
BmsI GCATC 1 cut(s) 46
BpiI GAAGAC 2 cut(s) 83, 472
BpuEI CTTGAG 2 cut(s) 348, 412
BsaAI YACGTR 1 cut(s) 632
BsaJI CCNNGG 1 cut(s) 270
BsaWI WCCGGW 1 cut(s) 904
Bsc4I CCNNNNNNNGG 5 cut(s) 112, 148, 417, 550, 566
Bse118I RCCGGY 1 cut(s) 130
Bse1I ACTGG 1 cut(s) 172
BseAI TCCGGA 1 cut(s) 904
BseBI CCWGG 2 cut(s) 107, 746
BseDI CCNNGG 1 cut(s) 270
BseLI CCNNNNNNNGG 5 cut(s) 112, 148, 417, 550, 566
BseMII CTCAG 3 cut(s) 346, 683, 706
BseNI ACTGG 1 cut(s) 172
BseRI GAGGAG 1 cut(s) 128
BseSI GKGCMC 1 cut(s) 143
BseXI GCAGC 2 cut(s) 139, 796
BsgI GTGCAG 1 cut(s) 146
BshFI GGCC 2 cut(s) 100, 171
BshNI GGYRCC 1 cut(s) 741
BsiSI CCGG 3 cut(s) 62, 131, 905
BslI CCNNNNNNNGG 5 cut(s) 112, 148, 417, 550, 566
BsmAI GTCTC 2 cut(s) 12, 269
BsmBI CGTCTC 1 cut(s) 12
BsmI GAATGC 2 cut(s) 392, 412
BsnI GGCC 2 cut(s) 100, 171
Bsp1286I GDGCHC 2 cut(s) 143, 712
Bsp13I TCCGGA 1 cut(s) 904
Bsp143I GATC 6 cut(s) 16, 30, 102, 253, 580, 859
BspACI CCGC 6 cut(s) 68, 156, 607, 666, 798, 864
BspANI GGCC 2 cut(s) 100, 171
BspCNI CTCAG 3 cut(s) 345, 682, 705
BspEI TCCGGA 1 cut(s) 904
BspHI TCATGA 1 cut(s) 343
BspLI GGNNCC 2 cut(s) 558, 743
BspMAI CTGCAG 1 cut(s) 855
BspPI GGATC 3 cut(s) 24, 97, 854
BspT107I GGYRCC 1 cut(s) 741
BsrBI CCGCTC 1 cut(s) 668
BsrFI RCCGGY 1 cut(s) 130
BsrI ACTGG 1 cut(s) 172
BssAI RCCGGY 1 cut(s) 130
BssECI CCNNGG 1 cut(s) 270
BssMI GATC 6 cut(s) 16, 30, 102, 253, 580, 859
BssNAI GTATAC 1 cut(s) 548
Bst1107I GTATAC 1 cut(s) 548
Bst2UI CCWGG 2 cut(s) 107, 746
Bst4CI ACNGT 6 cut(s) 26, 151, 233, 280, 545, 766
BstBAI YACGTR 1 cut(s) 632
BstC8I GCNNGC 2 cut(s) 89, 93
BstDEI CTNAG 3 cut(s) 332, 669, 692
BstDSI CCRYGG 1 cut(s) 270
BstENI CCTNNNNNAGG 1 cut(s) 110
BstH2I RGCGCY 1 cut(s) 777
BstHHI GCGC 1 cut(s) 776
BstKTI GATC 6 cut(s) 19, 33, 105, 256, 583, 862
BstMAI GTCTC 2 cut(s) 12, 269
BstMBI GATC 6 cut(s) 16, 30, 102, 253, 580, 859
BstMWI GCNNNNNNNGC 2 cut(s) 88, 97
BstNI CCWGG 2 cut(s) 107, 746
BstSCI CCNGG 2 cut(s) 105, 744
BstSFI CTRYAG 1 cut(s) 851
BstSLI GKGCMC 1 cut(s) 143
BstV1I GCAGC 2 cut(s) 139, 796
BstV2I GAAGAC 2 cut(s) 83, 472
BstX2I RGATCY 1 cut(s) 16
BstXI CCANNNNNNTGG 1 cut(s) 686
BstYI RGATCY 1 cut(s) 16
BstZ17I GTATAC 1 cut(s) 548
BsuI GTATCC 1 cut(s) 725
BsuRI GGCC 2 cut(s) 100, 171
BtgI CCRYGG 1 cut(s) 270
Cac8I GCNNGC 2 cut(s) 89, 93
CciI TCATGA 1 cut(s) 343
CfoI GCGC 1 cut(s) 776
Cfr10I RCCGGY 1 cut(s) 130
Cfr13I GGNCC 3 cut(s) 22, 541, 569
CseI GACGC 1 cut(s) 88
Csp6I GTAC 1 cut(s) 262
CviAII CATG 5 cut(s) 344, 563, 724, 805, 835
CviQI GTAC 1 cut(s) 262
DdeI CTNAG 3 cut(s) 332, 669, 692
DpnI GATC 6 cut(s) 18, 32, 104, 255, 582, 861
DpnII GATC 6 cut(s) 16, 30, 102, 253, 580, 859
DraIII CACNNNGTG 1 cut(s) 635
EaeI YGGCCR 1 cut(s) 169
EciI GGCGGA 1 cut(s) 83
Eco24I GRGCYC 1 cut(s) 712
Eco47I GGWCC 3 cut(s) 22, 541, 569
Eco47III AGCGCT 1 cut(s) 775
Eco72I CACGTG 1 cut(s) 632
EcoNI CCTNNNNNAGG 1 cut(s) 110
EcoRII CCWGG 2 cut(s) 105, 744
EcoT38I GRGCYC 1 cut(s) 712
Esp3I CGTCTC 1 cut(s) 12
FaeI CATG 5 cut(s) 347, 566, 727, 808, 838
FatI CATG 5 cut(s) 343, 562, 723, 804, 834
FauI CCCGC 2 cut(s) 791, 871
FblI GTMKAC 1 cut(s) 547
Fnu4HI GCNGC 3 cut(s) 128, 666, 810
FriOI GRGCYC 1 cut(s) 712
Fsp4HI GCNGC 3 cut(s) 128, 666, 810
GlaI GCGC 1 cut(s) 775
GluI GCNGC 3 cut(s) 128, 666, 810
HaeII RGCGCY 1 cut(s) 777
HaeIII GGCC 2 cut(s) 100, 171
HapII CCGG 3 cut(s) 62, 131, 905
HgaI GACGC 1 cut(s) 88
HhaI GCGC 1 cut(s) 776
Hin1II CATG 5 cut(s) 347, 566, 727, 808, 838
Hin6I GCGC 1 cut(s) 774
HinP1I GCGC 1 cut(s) 774
HinfI GANTC 4 cut(s) 349, 472, 690, 901
HpaII CCGG 3 cut(s) 62, 131, 905
HphI GGTGA 5 cut(s) 85, 145, 254, 495, 772
Hpy166II GTNNAC 1 cut(s) 548
Hpy188I TCNGA 7 cut(s) 16, 30, 42, 201, 335, 595, 699
Hpy188III TCNNGA 6 cut(s) 20, 218, 344, 418, 705, 905
Hpy8I GTNNAC 1 cut(s) 548
HpyAV CCTTC 2 cut(s) 560, 722
HpyCH4III ACNGT 6 cut(s) 26, 151, 233, 280, 545, 766
HpyCH4IV ACGT 3 cut(s) 56, 123, 631
HpyCH4V TGCA 5 cut(s) 51, 127, 490, 812, 853
HpyF10VI GCNNNNNNNGC 2 cut(s) 88, 97
HpyF3I CTNAG 3 cut(s) 332, 669, 692
HpySE526I ACGT 3 cut(s) 56, 123, 631
Hsp92II CATG 5 cut(s) 347, 566, 727, 808, 838
HspAI GCGC 1 cut(s) 774
Kpn2I TCCGGA 1 cut(s) 904
Kzo9I GATC 6 cut(s) 16, 30, 102, 253, 580, 859
LmnI GCTCC 2 cut(s) 220, 781
Lsp1109I GCAGC 2 cut(s) 139, 796
LweI GCATC 1 cut(s) 46
MaeII ACGT 3 cut(s) 56, 123, 631
MaeIII GTNAC 3 cut(s) 145, 280, 760
MalI GATC 6 cut(s) 18, 32, 104, 255, 582, 861
MbiI CCGCTC 1 cut(s) 668
MboI GATC 6 cut(s) 16, 30, 102, 253, 580, 859
MboII GAAGA 4 cut(s) 88, 440, 472, 763
MfeI CAATTG 1 cut(s) 736
MflI RGATCY 1 cut(s) 16
MhlI GDGCHC 2 cut(s) 143, 712
MluCI AATT 8 cut(s) 226, 321, 357, 736, 813, 822, 844, 876
MlyI GAGTC 4 cut(s) 358, 481, 684, 895
MroI TCCGGA 1 cut(s) 904
MseI TTAA 1 cut(s) 675
MslI CAYNNNNRTG 1 cut(s) 684
MspA1I CMGCKG 1 cut(s) 609
MspI CCGG 3 cut(s) 62, 131, 905
MspR9I CCNGG 2 cut(s) 107, 746
MunI CAATTG 1 cut(s) 736
Mva1269I GAATGC 2 cut(s) 392, 412
MvaI CCWGG 2 cut(s) 107, 746
MwoI GCNNNNNNNGC 2 cut(s) 88, 97
NdeII GATC 6 cut(s) 16, 30, 102, 253, 580, 859
NlaIII CATG 5 cut(s) 347, 566, 727, 808, 838
NlaIV GGNNCC 2 cut(s) 558, 743
NmuCI GTSAC 3 cut(s) 145, 280, 760
PagI TCATGA 1 cut(s) 343
PctI GAATGC 2 cut(s) 392, 412
PflFI GACNNNGTC 1 cut(s) 693
PflMI CCANNNNNTGG 1 cut(s) 417
PkrI GCNGC 3 cut(s) 129, 667, 811
PleI GAGTC 4 cut(s) 357, 480, 684, 895
PmaCI CACGTG 1 cut(s) 632
PmlI CACGTG 1 cut(s) 632
PpsI GAGTC 4 cut(s) 357, 480, 684, 895
Ppu21I YACGTR 1 cut(s) 632
Psp6I CCWGG 2 cut(s) 105, 744
PspCI CACGTG 1 cut(s) 632
PspGI CCWGG 2 cut(s) 105, 744
PspN4I GGNNCC 2 cut(s) 558, 743
PspPI GGNCC 3 cut(s) 22, 541, 569
PstI CTGCAG 1 cut(s) 855
PsuI RGATCY 1 cut(s) 16
PsyI GACNNNGTC 1 cut(s) 693
RsaI GTAC 1 cut(s) 263
RsaNI GTAC 1 cut(s) 262
RseI CAYNNNNRTG 1 cut(s) 684
SaqAI TTAA 1 cut(s) 675
SatI GCNGC 3 cut(s) 128, 666, 810
Sau3AI GATC 6 cut(s) 16, 30, 102, 253, 580, 859
Sau96I GGNCC 3 cut(s) 22, 541, 569
SchI GAGTC 4 cut(s) 358, 481, 684, 895
ScrFI CCNGG 2 cut(s) 107, 746
SduI GDGCHC 2 cut(s) 143, 712
SfaNI GCATC 1 cut(s) 46
SfcI CTRYAG 1 cut(s) 851
SinI GGWCC 3 cut(s) 22, 541, 569
SmiMI CAYNNNNRTG 1 cut(s) 684
SmlI CTYRAG 2 cut(s) 363, 391
SmoI CTYRAG 2 cut(s) 363, 391
Sse9I AATT 8 cut(s) 226, 321, 357, 736, 813, 822, 844, 876
SsiI CCGC 6 cut(s) 68, 156, 607, 666, 798, 864
SspI AATATT 1 cut(s) 529
StyD4I CCNGG 2 cut(s) 105, 744
TaaI ACNGT 6 cut(s) 26, 151, 233, 280, 545, 766
TaiI ACGT 3 cut(s) 59, 126, 634
TaqI TCGA 2 cut(s) 33, 532
TasI AATT 8 cut(s) 226, 321, 357, 736, 813, 822, 844, 876
TauI GCSGC 1 cut(s) 668
Tru1I TTAA 1 cut(s) 675
Tru9I TTAA 1 cut(s) 675
TseFI GTSAC 3 cut(s) 145, 280, 760
TseI GCWGC 2 cut(s) 127, 809
Tsp45I GTSAC 3 cut(s) 145, 280, 760
TspDTI ATGAA 1 cut(s) 579
TspGWI ACGGA 2 cut(s) 259, 296
Tth111I GACNNNGTC 1 cut(s) 693
Van91I CCANNNNNTGG 1 cut(s) 417
VpaK11BI GGWCC 3 cut(s) 22, 541, 569
XagI CCTNNNNNAGG 1 cut(s) 110
XapI RAATTY 1 cut(s) 844
XmiI GTMKAC 1 cut(s) 547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.