pycom16g07950

Protein of unknown function (DUF1645)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0012761
Physical Location & Seq
Reverse (-)
5262283 .. 5263197
915 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 915 bp
ATGCAAGCGGTTTCCGTGTTTTCCGTGTCGCCGAGCTTCAATTCCAACGATTCCGTGAACGATTTCGAAGCCGAATACGCGCAGCTCTCTGCGAATTTCAGCTCCCAGCTCCAAGTCGCCGGTTCCGGCGGCGATTTTGAATTCGAGGACGCTGAGAAGGTAGAAATGGAAGATAAAGAGGCTCAAAGCGTGGACCACGGCGGCGAATGCGAGAGCGAAAACGAAGACGATTTCTCTTTCGCCCCCACCAACGCCGACGGTTCGCCGATTTCCGCGGACGATATATTCCAAAACGGCCAGATCCGGCCGGTATTTCCGATTTTCAACCGAGATCTTCTCTTCGCCGAAGCCGACGACGGCGATGCTTCCAGAGCCAGAGCGGCCTCGTCTGCAACTTCGTCCTCGTCTCTAAGGCCGCCGTTGAAGAGGCTCTTCTTCGAAGAGCGGGACACGCAGCCGTCGTCGGCGTCCGAGTTGGACGAGCTCGAAGGAGTCCCGGAAGGAACGTTCTGCGAGTGGTCAAGGAAGCCGGTGGAGGCGGCGCCGGAGCTCCGGAACAAGAGCAATTCCACGGGATCCTCGAAGCTGTGGAGAGTCAGAGACTTGAAGCTCCGGAGCAACAGCGACGGCAAAGACGCTTTCGTCTTCCTGAACCCTAAGTCGGCGGCGAGCCCGAAGCCGAGCCAGGAATCGGCGGCGGATGGGAAGAGCAACGCTGAGATTCAAAAGACGGCGGAGAAGGTAAAGGGGAAGGCTAAGAAAGTTGAAACGGTATCGTCTGCGCACGAGAAACATTACGTGAAGAACAGAGAGAAGAAGGAGGGGGATAAACGGCGGTCGTACTTGCCGTATCGGCCGGTGGTTGGGTTCTTCACCAATGTGAACGTATTGAGCAGAAACGTTCATCCTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1645

Protein Analysis

305

Amino Acids

33.44

Weight (kDa)

5.01

Isoelectric Point (pI)

47.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1645 PF07816 92 - 283 2.4e-31 Protein of unknown function (DUF1645)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012761)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23710 AT1G70420
fragaria_vesca FvH4_4g34370
malus_domestica MD13G1092700.v1.1 MD16G1093500.v1.1
prunus_persica Prupe.1G253100_v2.0.a1
pyrus_communis pycom13g08060 pycom16g07950
rosa_chinensis RchiOBHm_Chr4g0443781
rosa_laevigata RLG00000005889
rosa_multiflora Rmu_sc0005632.1_g000001 Rmu_sc0036070.1_g000002
rosa_roxburghii Rroxscaffold_5G00384360
rosa_rugosa Rorug04G0347200
rosa_samantha Rh4BG418400 Rh4CG432600 Rh4DG413700
rosa_wichuraiana Rw4G035000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 641
Acc16I TGCGCA 1 cut(s) 783
AccB1I GGYRCC 1 cut(s) 541
AccBSI CCGCTC 2 cut(s) 380, 445
AccII CGCG 2 cut(s) 80, 275
AccIII TCCGGA 2 cut(s) 552, 612
AclI AACGTT 2 cut(s) 506, 900
AclWI GGATC 3 cut(s) 295, 570, 583
AcoI YGGCCR 3 cut(s) 295, 305, 854
AcsI RAATTY 2 cut(s) 94, 140
AcyI GRCGYC 2 cut(s) 467, 542
AfaI GTAC 1 cut(s) 842
AfiI CCNNNNNNNGG 3 cut(s) 661, 691, 863
AgsI TTSAA 7 cut(s) 40, 140, 325, 424, 607, 725, 767
AjnI CCWGG 1 cut(s) 684
AleI CACNNNNGTG 1 cut(s) 878
AloI GAACNNNNNNTCC 2 cut(s) 491, 523
AluBI AGCT 8 cut(s) 36, 85, 102, 109, 484, 550, 586, 610
AluI AGCT 8 cut(s) 36, 85, 102, 109, 484, 550, 586, 610
Alw21I GWGCWC 2 cut(s) 486, 552
Alw26I GTCTC 2 cut(s) 411, 594
AlwI GGATC 3 cut(s) 295, 570, 583
Aor13HI TCCGGA 2 cut(s) 552, 612
AoxI GGCC 5 cut(s) 295, 305, 381, 413, 854
ApeKI GCWGC 2 cut(s) 82, 454
ApoI RAATTY 2 cut(s) 94, 140
Asp700I GAANNNNTTC 1 cut(s) 62
AspLEI GCGC 3 cut(s) 82, 544, 784
AspS9I GGNCC 1 cut(s) 193
AsuC2I CCSGG 1 cut(s) 497
AsuHPI GGTGA 1 cut(s) 865
AsuII TTCGAA 2 cut(s) 66, 438
AvaII GGWCC 1 cut(s) 193
BamHI GGATCC 1 cut(s) 575
BanI GGYRCC 1 cut(s) 541
BanII GRGCYC 3 cut(s) 486, 552, 674
BauI CACGAG 1 cut(s) 785
BbsI GAAGAC 2 cut(s) 231, 637
Bbv12I GWGCWC 2 cut(s) 486, 552
BbvI GCAGC 2 cut(s) 94, 466
BccI CCATC 1 cut(s) 695
BceAI ACGGC 9 cut(s) 214, 310, 373, 403, 442, 643, 747, 832, 848
BcgI CGANNNNNNTGC 2 cut(s) 344, 378
BciT130I CCWGG 1 cut(s) 686
BcnI CCSGG 1 cut(s) 497
BcoDI GTCTC 2 cut(s) 411, 594
BfoI RGCGCY 1 cut(s) 545
BglI GCCNNNNNGGC 2 cut(s) 380, 853
BglII AGATCT 1 cut(s) 331
BisI GCNGC 9 cut(s) 83, 130, 202, 381, 416, 455, 540, 666, 696
BlsI GCNGC 9 cut(s) 84, 131, 203, 382, 417, 456, 541, 667, 697
Bme1390I CCNGG 2 cut(s) 497, 686
Bme18I GGWCC 1 cut(s) 193
BmgT120I GGNCC 1 cut(s) 193
BmiI GGNNCC 3 cut(s) 124, 543, 577
BmrFI CCNGG 2 cut(s) 497, 686
BmsI GCATC 1 cut(s) 352
BpiI GAAGAC 2 cut(s) 231, 637
BplI GAGNNNNNCTC 2 cut(s) 321, 353
Bpu14I TTCGAA 2 cut(s) 66, 438
BpuMI CCSGG 1 cut(s) 497
BsaAI YACGTR 1 cut(s) 799
BsaHI GRCGYC 2 cut(s) 467, 542
BsaJI CCNNGG 3 cut(s) 196, 273, 570
BsaWI WCCGGW 2 cut(s) 552, 612
Bsc4I CCNNNNNNNGG 3 cut(s) 661, 691, 863
Bse118I RCCGGY 4 cut(s) 119, 307, 529, 856
BseAI TCCGGA 2 cut(s) 552, 612
BseBI CCWGG 1 cut(s) 686
BseDI CCNNGG 3 cut(s) 196, 273, 570
BseGI GGATG 2 cut(s) 706, 904
BseLI CCNNNNNNNGG 3 cut(s) 661, 691, 863
BseMII CTCAG 2 cut(s) 144, 708
BseX3I CGGCCG 2 cut(s) 305, 854
BseXI GCAGC 2 cut(s) 94, 466
BseYI CCCAGC 1 cut(s) 105
Bsh1236I CGCG 2 cut(s) 80, 275
Bsh1285I CGRYCG 3 cut(s) 308, 839, 857
BshFI GGCC 5 cut(s) 297, 307, 383, 415, 856
BshNI GGYRCC 1 cut(s) 541
BsiEI CGRYCG 3 cut(s) 308, 839, 857
BsiHKAI GWGCWC 2 cut(s) 486, 552
BslFI GGGAC 2 cut(s) 461, 479
BslI CCNNNNNNNGG 3 cut(s) 661, 691, 863
BsmAI GTCTC 2 cut(s) 411, 594
BsmBI CGTCTC 1 cut(s) 411
BsmFI GGGAC 2 cut(s) 461, 479
BsmI GAATGC 1 cut(s) 212
BsnI GGCC 5 cut(s) 297, 307, 383, 415, 856
Bsp119I TTCGAA 2 cut(s) 66, 438
Bsp1286I GDGCHC 3 cut(s) 486, 552, 674
Bsp13I TCCGGA 2 cut(s) 552, 612
Bsp143I GATC 3 cut(s) 300, 331, 575
BspANI GGCC 5 cut(s) 297, 307, 383, 415, 856
BspCNI CTCAG 2 cut(s) 145, 709
BspEI TCCGGA 2 cut(s) 552, 612
BspFNI CGCG 2 cut(s) 80, 275
BspLI GGNNCC 3 cut(s) 124, 543, 577
BspPI GGATC 3 cut(s) 295, 570, 583
BspQI GCTCTTC 3 cut(s) 435, 437, 701
BspT104I TTCGAA 2 cut(s) 66, 438
BspT107I GGYRCC 1 cut(s) 541
BsrBI CCGCTC 2 cut(s) 380, 445
BsrFI RCCGGY 4 cut(s) 119, 307, 529, 856
BssAI RCCGGY 4 cut(s) 119, 307, 529, 856
BssECI CCNNGG 3 cut(s) 196, 273, 570
BssMI GATC 3 cut(s) 300, 331, 575
BssNI GRCGYC 2 cut(s) 467, 542
BssSI CACGAG 1 cut(s) 785
Bst2BI CACGAG 1 cut(s) 785
Bst2UI CCWGG 1 cut(s) 686
Bst4CI ACNGT 2 cut(s) 260, 772
Bst6I CTCTTC 5 cut(s) 344, 419, 435, 437, 701
BstACI GRCGYC 2 cut(s) 467, 542
BstBAI YACGTR 1 cut(s) 799
BstBI TTCGAA 2 cut(s) 66, 438
BstC8I GCNNGC 2 cut(s) 6, 670
BstDEI CTNAG 5 cut(s) 153, 410, 657, 717, 756
BstDSI CCRYGG 3 cut(s) 196, 273, 570
BstF5I GGATG 2 cut(s) 706, 904
BstFNI CGCG 2 cut(s) 80, 275
BstH2I RGCGCY 1 cut(s) 545
BstHHI GCGC 3 cut(s) 82, 544, 784
BstKTI GATC 3 cut(s) 303, 334, 578
BstMAI GTCTC 2 cut(s) 411, 594
BstMBI GATC 3 cut(s) 300, 331, 575
BstMCI CGRYCG 3 cut(s) 308, 839, 857
BstMWI GCNNNNNNNGC 7 cut(s) 77, 207, 371, 380, 389, 451, 853
BstNI CCWGG 1 cut(s) 686
BstSCI CCNGG 2 cut(s) 495, 684
BstUI CGCG 2 cut(s) 80, 275
BstV1I GCAGC 2 cut(s) 94, 466
BstV2I GAAGAC 2 cut(s) 231, 637
BstX2I RGATCY 3 cut(s) 300, 331, 575
BstYI RGATCY 3 cut(s) 300, 331, 575
BstZI CGGCCG 2 cut(s) 305, 854
BsuRI GGCC 5 cut(s) 297, 307, 383, 415, 856
BtgI CCRYGG 3 cut(s) 196, 273, 570
BtgZI GCGATG 1 cut(s) 375
BtsCI GGATG 2 cut(s) 706, 904
Cac8I GCNNGC 2 cut(s) 6, 670
CfoI GCGC 3 cut(s) 82, 544, 784
Cfr10I RCCGGY 4 cut(s) 119, 307, 529, 856
Cfr13I GGNCC 1 cut(s) 193
Cfr42I CCGCGG 1 cut(s) 276
CseI GACGC 3 cut(s) 158, 456, 644
Csp6I GTAC 1 cut(s) 841
CviQI GTAC 1 cut(s) 841
DdeI CTNAG 5 cut(s) 153, 410, 657, 717, 756
DinI GGCGCC 1 cut(s) 543
DpnI GATC 3 cut(s) 302, 333, 577
DpnII GATC 3 cut(s) 300, 331, 575
DrdI GACNNNNNNGTC 1 cut(s) 641
DseDI GACNNNNNNGTC 1 cut(s) 641
EaeI YGGCCR 3 cut(s) 295, 305, 854
EagI CGGCCG 2 cut(s) 305, 854
Eam1104I CTCTTC 5 cut(s) 344, 419, 435, 437, 701
EarI CTCTTC 5 cut(s) 344, 419, 435, 437, 701
EciI GGCGGA 2 cut(s) 713, 749
Ecl136II GAGCTC 2 cut(s) 484, 550
EclXI CGGCCG 2 cut(s) 305, 854
Eco24I GRGCYC 3 cut(s) 486, 552, 674
Eco47I GGWCC 1 cut(s) 193
Eco52I CGGCCG 2 cut(s) 305, 854
Eco53kI GAGCTC 2 cut(s) 484, 550
EcoICRI GAGCTC 2 cut(s) 484, 550
EcoRI GAATTC 1 cut(s) 140
EcoRII CCWGG 1 cut(s) 684
EcoT38I GRGCYC 3 cut(s) 486, 552, 674
EgeI GGCGCC 1 cut(s) 543
EheI GGCGCC 1 cut(s) 543
Esp3I CGTCTC 1 cut(s) 411
FaiI YATR 1 cut(s) 284
FalI AAGNNNNNCTT 2 cut(s) 416, 448
FaqI GGGAC 2 cut(s) 461, 479
FauI CCCGC 1 cut(s) 438
Fnu4HI GCNGC 9 cut(s) 83, 130, 202, 381, 416, 455, 540, 666, 696
FokI GGATG 2 cut(s) 713, 891
FriOI GRGCYC 3 cut(s) 486, 552, 674
Fsp4HI GCNGC 9 cut(s) 83, 130, 202, 381, 416, 455, 540, 666, 696
FspI TGCGCA 1 cut(s) 783
GlaI GCGC 3 cut(s) 81, 543, 783
GluI GCNGC 9 cut(s) 83, 130, 202, 381, 416, 455, 540, 666, 696
GsaI CCCAGC 1 cut(s) 109
HaeII RGCGCY 1 cut(s) 545
HaeIII GGCC 5 cut(s) 297, 307, 383, 415, 856
HgaI GACGC 3 cut(s) 158, 456, 644
HhaI GCGC 3 cut(s) 82, 544, 784
Hin1I GRCGYC 2 cut(s) 467, 542
Hin6I GCGC 3 cut(s) 80, 542, 782
HinP1I GCGC 3 cut(s) 80, 542, 782
HinfI GANTC 5 cut(s) 50, 492, 594, 689, 721
HphI GGTGA 1 cut(s) 865
Hpy166II GTNNAC 3 cut(s) 58, 193, 883
Hpy188I TCNGA 4 cut(s) 318, 472, 599, 914
Hpy188III TCNNGA 4 cut(s) 369, 553, 613, 649
Hpy8I GTNNAC 3 cut(s) 58, 193, 883
Hpy99I CGWCG 6 cut(s) 260, 356, 359, 463, 466, 629
HpyAV CCTTC 6 cut(s) 151, 482, 494, 733, 745, 811
HpyCH4III ACNGT 2 cut(s) 260, 772
HpyCH4IV ACGT 4 cut(s) 506, 798, 885, 900
HpyCH4V TGCA 2 cut(s) 4, 392
HpyF10VI GCNNNNNNNGC 7 cut(s) 77, 207, 371, 380, 389, 451, 853
HpyF3I CTNAG 5 cut(s) 153, 410, 657, 717, 756
HpySE526I ACGT 4 cut(s) 506, 798, 885, 900
Hsp92I GRCGYC 2 cut(s) 467, 542
HspAI GCGC 3 cut(s) 80, 542, 782
KasI GGCGCC 1 cut(s) 541
Kpn2I TCCGGA 2 cut(s) 552, 612
KspI CCGCGG 1 cut(s) 276
Kzo9I GATC 3 cut(s) 300, 331, 575
LguI GCTCTTC 3 cut(s) 435, 437, 701
LmnI GCTCC 6 cut(s) 107, 114, 547, 555, 615, 615
Lsp1109I GCAGC 2 cut(s) 94, 466
LweI GCATC 1 cut(s) 352
MaeII ACGT 4 cut(s) 506, 798, 885, 900
MalI GATC 3 cut(s) 302, 333, 577
MbiI CCGCTC 2 cut(s) 380, 445
MboI GATC 3 cut(s) 300, 331, 575
MflI RGATCY 3 cut(s) 300, 331, 575
MhlI GDGCHC 3 cut(s) 486, 552, 674
MluCI AATT 4 cut(s) 40, 94, 140, 565
Mly113I GGCGCC 1 cut(s) 542
MlyI GAGTC 2 cut(s) 501, 603
MmeI TCCRAC 2 cut(s) 69, 456
MnlI CCTC 8 cut(s) 139, 172, 394, 412, 420, 529, 589, 814
MroI TCCGGA 2 cut(s) 552, 612
MroXI GAANNNNTTC 1 cut(s) 62
MslI CAYNNNNRTG 1 cut(s) 878
MspA1I CMGCKG 1 cut(s) 275
MspR9I CCNGG 2 cut(s) 497, 686
Mva1269I GAATGC 1 cut(s) 212
MvaI CCWGG 1 cut(s) 686
MvnI CGCG 2 cut(s) 80, 275
MwoI GCNNNNNNNGC 7 cut(s) 77, 207, 371, 380, 389, 451, 853
NarI GGCGCC 1 cut(s) 542
NciI CCSGG 1 cut(s) 497
NdeII GATC 3 cut(s) 300, 331, 575
NlaIV GGNNCC 3 cut(s) 124, 543, 577
NmeAIII GCCGAG 2 cut(s) 57, 705
NsbI TGCGCA 1 cut(s) 783
NspV TTCGAA 2 cut(s) 66, 438
OliI CACNNNNGTG 1 cut(s) 878
PciSI GCTCTTC 3 cut(s) 435, 437, 701
PcsI WCGNNNNNNNCGW 4 cut(s) 348, 458, 578, 845
PctI GAATGC 1 cut(s) 212
PdmI GAANNNNTTC 1 cut(s) 62
PfeI GAWTC 3 cut(s) 50, 689, 721
PfoI TCCNGGA 1 cut(s) 495
PkrI GCNGC 9 cut(s) 84, 131, 203, 382, 417, 456, 541, 667, 697
PleI GAGTC 2 cut(s) 500, 602
PluTI GGCGCC 1 cut(s) 545
PpsI GAGTC 2 cut(s) 500, 602
Ppu21I YACGTR 1 cut(s) 799
Psp124BI GAGCTC 2 cut(s) 486, 552
Psp1406I AACGTT 2 cut(s) 506, 900
Psp6I CCWGG 1 cut(s) 684
PspFI CCCAGC 1 cut(s) 105
PspGI CCWGG 1 cut(s) 684
PspN4I GGNNCC 3 cut(s) 124, 543, 577
PspPI GGNCC 1 cut(s) 193
PsuI RGATCY 3 cut(s) 300, 331, 575
RsaI GTAC 1 cut(s) 842
RsaNI GTAC 1 cut(s) 841
RseI CAYNNNNRTG 1 cut(s) 878
SacI GAGCTC 2 cut(s) 486, 552
SacII CCGCGG 1 cut(s) 276
SapI GCTCTTC 3 cut(s) 435, 437, 701
SatI GCNGC 9 cut(s) 83, 130, 202, 381, 416, 455, 540, 666, 696
Sau3AI GATC 3 cut(s) 300, 331, 575
Sau96I GGNCC 1 cut(s) 193
SchI GAGTC 2 cut(s) 501, 603
ScrFI CCNGG 2 cut(s) 497, 686
SduI GDGCHC 3 cut(s) 486, 552, 674
SfaNI GCATC 1 cut(s) 352
SfoI GGCGCC 1 cut(s) 543
Sfr303I CCGCGG 1 cut(s) 276
SfuI TTCGAA 2 cut(s) 66, 438
SgrBI CCGCGG 1 cut(s) 276
SinI GGWCC 1 cut(s) 193
SmiMI CAYNNNNRTG 1 cut(s) 878
Sse9I AATT 4 cut(s) 40, 94, 140, 565
SspDI GGCGCC 1 cut(s) 541
SstI GAGCTC 2 cut(s) 486, 552
StyD4I CCNGG 2 cut(s) 495, 684
TaaI ACNGT 2 cut(s) 260, 772
TaiI ACGT 4 cut(s) 509, 801, 888, 903
TaqI TCGA 5 cut(s) 66, 144, 438, 486, 581
TasI AATT 4 cut(s) 40, 94, 140, 565
TauI GCSGC 7 cut(s) 132, 204, 383, 418, 542, 668, 698
TfiI GAWTC 3 cut(s) 50, 689, 721
TseI GCWGC 2 cut(s) 82, 454
TspDTI ATGAA 1 cut(s) 893
TspGWI ACGGA 3 cut(s) 4, 13, 43
VpaK11BI GGWCC 1 cut(s) 193
XapI RAATTY 2 cut(s) 94, 140
XmnI GAANNNNTTC 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.