pycom16g09560

Protein of unknown function (DUF3537)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0007442
Physical Location & Seq
Reverse (-)
6377294 .. 6379158
1865 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1266 bp
ATGGCGGACGTTTTTATAAAAACAGAGGAGCCACCTTCCCAACTTATCCCTCTTCTCGATTCTAGTAACAATGACAACCACCACCACAACAACCCCCACAGCAACCGTCACCTGGGCGGAGAAGAAGAATACAAAGGTACCCCATTGGACCAAACCCTCCAATGGCTAGAAACGTTTCTCACTCTCTTGGGCTTCAACCAATCTTCTTGGTTGAGCCTTGTCCTGTCCTGGACGGCTCTGGCTCTCATCGGTGTGGCTCTTCCGGTGGTTGTGCTTGTGGTGTTCAACTGCTCCGACTGTAAGAAGTATCAGATTAAAAGGTTCGAGCTCGTTATCGTGGGCTCGCAGGCGATCCTGGCGGCGGTCTCTGTGCTCTGCATCTCACCCAGACTTCGCAAGTATGGCATTCGGAGGTTCCTCTTTGTCGATCGTTATAGCGGCCATATCGTTCGGTTTCGAAACGATTACATCAAGCAGATTAAGGATTCTTTCCGATTGCTTGTTCGGTGGGCACTGCCATGTTTTCTTTTGAAGACACTGCGTGAGGTCGTCCGCATGATATACGTGCATCACGATTCATGGGGGTGGGGGAAATCAGTTGGTGTATTGTTCACTTTGGTTCTATCGTGGGCTTATGTGAGTACAATCACTCTAACAGCCAGCATTTTGTTTCACTTGGTCTGCAATTTGCAAGTTATCCACTTCGACGATTATGCAAGGCTCTTGGAAAGGGAATCTGATGTGCTGGTATTTATGGACGAGCATATTCGTCTGCGCTACCATCTCTGTAAAATAAGCCACAGGTTCCGAATCTTCCTTCTCCTGCAGTTCATAGTTGTCACCGCAAGCCAGTTTGTCACTCTATTTGAGACCACAGAATACAGGGGAGACATCACTGTCATAAATGGCGGCGATTTTGCAGTCTCCACAATTGTTCAAGTTGTGGGCATTATTCTCTCCCTGCACGCAGCGACCAGAATTTCTCATAGAGCGCAAGGCATAGTGACAGTTGCCAGTAGATGGCATGCGTTAATGACATGCAGTTCTAACGATACATCTCACAACAGAAGTTCAAACTGCTTGGTGAACCCAGAGGCTGCCGACACATTGAACTCGCTGCAAATAAGCTACTCCGAGAGTGACTTGGAGTCGATGGATTACGTTCCAATGCCTACGAATACACAGCTAGCTTCGTACATGTCTTCCTATCACAGGAGACAAGCTTTCGGTATGGATCAGCTTACACTGCCTCTAAAATACATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3537

Protein Analysis

422

Amino Acids

48.13

Weight (kDa)

8.09

Isoelectric Point (pI)

39.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3537 PF12056 52 - 409 3.5e-127 Protein of unknown function (DUF3537)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 17
AasI GACNNNNNNGTC 1 cut(s) 896
Acc65I GGTACC 1 cut(s) 137
AccB1I GGYRCC 1 cut(s) 137
AccB7I CCANNNNNTGG 1 cut(s) 1020
AciI CCGC 8 cut(s) 5, 117, 359, 362, 438, 553, 843, 909
AclI AACGTT 1 cut(s) 173
AclWI GGATC 2 cut(s) 346, 1242
AcoI YGGCCR 1 cut(s) 439
AcsI RAATTY 1 cut(s) 978
AdeI CACNNNGTG 1 cut(s) 542
AfaI GTAC 3 cut(s) 139, 643, 1196
AfiI CCNNNNNNNGG 5 cut(s) 112, 162, 361, 1020, 1212
AflIII ACRYGT 1 cut(s) 1197
AgsI TTSAA 6 cut(s) 196, 286, 532, 938, 1074, 1111
AhdI GACNNNNNGTC 1 cut(s) 1147
AjnI CCWGG 3 cut(s) 111, 227, 354
AluBI AGCT 6 cut(s) 328, 1128, 1186, 1190, 1223, 1240
AluI AGCT 6 cut(s) 328, 1128, 1186, 1190, 1223, 1240
Alw21I GWGCWC 2 cut(s) 330, 375
Alw26I GTCTC 5 cut(s) 370, 863, 882, 928, 1210
AlwI GGATC 2 cut(s) 346, 1242
AlwNI CAGNNNCTG 1 cut(s) 1097
AoxI GGCC 1 cut(s) 439
ApeKI GCWGC 3 cut(s) 968, 1097, 1117
ApoI RAATTY 1 cut(s) 978
Asp700I GAANNNNTTC 1 cut(s) 174
Asp718I GGTACC 1 cut(s) 137
AspLEI GCGC 2 cut(s) 777, 994
AspS9I GGNCC 1 cut(s) 148
AsuHPI GGTGA 4 cut(s) 101, 375, 832, 1096
AsuII TTCGAA 1 cut(s) 457
AsuNHI GCTAGC 1 cut(s) 1186
AvaII GGWCC 1 cut(s) 148
BaeGI GKGCMC 1 cut(s) 514
BaeI ACNNNNGTAYC 2 cut(s) 121, 154
BanI GGYRCC 1 cut(s) 137
BanII GRGCYC 2 cut(s) 330, 344
BbsI GAAGAC 2 cut(s) 539, 1194
Bbv12I GWGCWC 2 cut(s) 330, 375
BbvI GCAGC 3 cut(s) 980, 1084, 1104
BccI CCATC 3 cut(s) 789, 1014, 1147
BceAI ACGGC 1 cut(s) 249
BcgI CGANNNNNNTGC 2 cut(s) 695, 729
BciT130I CCWGG 3 cut(s) 113, 229, 356
BcoDI GTCTC 5 cut(s) 370, 863, 882, 928, 1210
BfaI CTAG 3 cut(s) 63, 167, 1187
BfmI CTRYAG 1 cut(s) 824
BisI GCNGC 6 cut(s) 360, 439, 910, 969, 1098, 1118
BlsI GCNGC 6 cut(s) 361, 440, 911, 970, 1099, 1119
Bme1390I CCNGG 3 cut(s) 113, 229, 356
Bme18I GGWCC 1 cut(s) 148
BmeRI GACNNNNNGTC 1 cut(s) 1147
BmgT120I GGNCC 1 cut(s) 148
BmiI GGNNCC 4 cut(s) 30, 139, 416, 806
BmrFI CCNGG 3 cut(s) 113, 229, 356
BmsI GCATC 2 cut(s) 387, 577
BmtI GCTAGC 1 cut(s) 1190
BpiI GAAGAC 2 cut(s) 539, 1194
Bpu14I TTCGAA 1 cut(s) 457
BsaAI YACGTR 1 cut(s) 565
BsaI GGTCTC 2 cut(s) 370, 863
BsaJI CCNNGG 1 cut(s) 112
BsaWI WCCGGW 1 cut(s) 262
Bsc4I CCNNNNNNNGG 5 cut(s) 112, 162, 361, 1020, 1212
Bse1I ACTGG 2 cut(s) 850, 1014
BseBI CCWGG 3 cut(s) 113, 229, 356
BseDI CCNNGG 1 cut(s) 112
BseLI CCNNNNNNNGG 5 cut(s) 112, 162, 361, 1020, 1212
BseNI ACTGG 2 cut(s) 850, 1014
BseRI GAGGAG 1 cut(s) 41
BseSI GKGCMC 1 cut(s) 514
BseXI GCAGC 3 cut(s) 980, 1084, 1104
BsgI GTGCAG 1 cut(s) 947
Bsh1285I CGRYCG 1 cut(s) 430
BshFI GGCC 1 cut(s) 441
BshNI GGYRCC 1 cut(s) 137
BsiEI CGRYCG 1 cut(s) 430
BsiHKAI GWGCWC 2 cut(s) 330, 375
BsiSI CCGG 1 cut(s) 263
BslI CCNNNNNNNGG 5 cut(s) 112, 162, 361, 1020, 1212
BsmAI GTCTC 5 cut(s) 370, 863, 882, 928, 1210
BsmI GAATGC 1 cut(s) 405
BsnI GGCC 1 cut(s) 441
Bso31I GGTCTC 2 cut(s) 370, 863
Bsp119I TTCGAA 1 cut(s) 457
Bsp1286I GDGCHC 4 cut(s) 330, 344, 375, 514
Bsp143I GATC 3 cut(s) 351, 427, 1234
BspACI CCGC 8 cut(s) 5, 117, 359, 362, 438, 553, 843, 909
BspANI GGCC 1 cut(s) 441
BspLI GGNNCC 4 cut(s) 30, 139, 416, 806
BspMAI CTGCAG 1 cut(s) 828
BspOI GCTAGC 1 cut(s) 1190
BspPI GGATC 2 cut(s) 346, 1242
BspQI GCTCTTC 1 cut(s) 264
BspT104I TTCGAA 1 cut(s) 457
BspT107I GGYRCC 1 cut(s) 137
BspTNI GGTCTC 2 cut(s) 370, 863
BsrI ACTGG 2 cut(s) 850, 1014
BssECI CCNNGG 1 cut(s) 112
BssMI GATC 3 cut(s) 351, 427, 1234
Bst2UI CCWGG 3 cut(s) 113, 229, 356
Bst4CI ACNGT 4 cut(s) 107, 299, 898, 1009
Bst6I CTCTTC 2 cut(s) 57, 264
BstBAI YACGTR 1 cut(s) 565
BstBI TTCGAA 1 cut(s) 457
BstC8I GCNNGC 7 cut(s) 344, 348, 661, 847, 966, 1026, 1188
BstENI CCTNNNNNAGG 1 cut(s) 1210
BstHHI GCGC 2 cut(s) 777, 994
BstKTI GATC 3 cut(s) 354, 430, 1237
BstMAI GTCTC 5 cut(s) 370, 863, 882, 928, 1210
BstMBI GATC 3 cut(s) 351, 427, 1234
BstMCI CGRYCG 1 cut(s) 430
BstMWI GCNNNNNNNGC 3 cut(s) 356, 402, 1246
BstNI CCWGG 3 cut(s) 113, 229, 356
BstNSI RCATGY 3 cut(s) 1028, 1041, 1201
BstSCI CCNGG 3 cut(s) 111, 227, 354
BstSFI CTRYAG 1 cut(s) 824
BstSLI GKGCMC 1 cut(s) 514
BstV1I GCAGC 3 cut(s) 980, 1084, 1104
BstV2I GAAGAC 2 cut(s) 539, 1194
BsuRI GGCC 1 cut(s) 441
BtsI GCAGTG 3 cut(s) 512, 536, 1244
BtsIMutI CAGTG 4 cut(s) 512, 536, 894, 1244
Cac8I GCNNGC 7 cut(s) 344, 348, 661, 847, 966, 1026, 1188
CaiI CAGNNNCTG 1 cut(s) 1097
CfoI GCGC 2 cut(s) 777, 994
Cfr13I GGNCC 1 cut(s) 148
Csp6I GTAC 3 cut(s) 138, 642, 1195
CviAII CATG 6 cut(s) 519, 556, 579, 1025, 1038, 1198
CviQI GTAC 3 cut(s) 138, 642, 1195
DpnI GATC 3 cut(s) 353, 429, 1236
DpnII GATC 3 cut(s) 351, 427, 1234
DraIII CACNNNGTG 1 cut(s) 542
DrdI GACNNNNNNGTC 1 cut(s) 896
DriI GACNNNNNGTC 1 cut(s) 1147
DseDI GACNNNNNNGTC 1 cut(s) 896
EaeI YGGCCR 1 cut(s) 439
Eam1104I CTCTTC 2 cut(s) 57, 264
Eam1105I GACNNNNNGTC 1 cut(s) 1147
EarI CTCTTC 2 cut(s) 57, 264
EciI GGCGGA 2 cut(s) 20, 132
Ecl136II GAGCTC 1 cut(s) 328
Eco24I GRGCYC 2 cut(s) 330, 344
Eco31I GGTCTC 2 cut(s) 370, 863
Eco47I GGWCC 1 cut(s) 148
Eco53kI GAGCTC 1 cut(s) 328
EcoICRI GAGCTC 1 cut(s) 328
EcoNI CCTNNNNNAGG 1 cut(s) 1210
EcoRII CCWGG 3 cut(s) 111, 227, 354
EcoT38I GRGCYC 2 cut(s) 330, 344
FaeI CATG 6 cut(s) 522, 559, 582, 1028, 1041, 1201
FatI CATG 6 cut(s) 518, 555, 578, 1024, 1037, 1197
Fnu4HI GCNGC 6 cut(s) 360, 439, 910, 969, 1098, 1118
FriOI GRGCYC 2 cut(s) 330, 344
Fsp4HI GCNGC 6 cut(s) 360, 439, 910, 969, 1098, 1118
FspBI CTAG 3 cut(s) 63, 167, 1187
GlaI GCGC 2 cut(s) 776, 993
GluI GCNGC 6 cut(s) 360, 439, 910, 969, 1098, 1118
HaeIII GGCC 1 cut(s) 441
HapII CCGG 1 cut(s) 263
HhaI GCGC 2 cut(s) 777, 994
Hin1II CATG 6 cut(s) 522, 559, 582, 1028, 1041, 1201
Hin6I GCGC 2 cut(s) 775, 992
HinP1I GCGC 2 cut(s) 775, 992
HindIII AAGCTT 1 cut(s) 1221
HinfI GANTC 6 cut(s) 59, 485, 575, 734, 810, 1148
HpaII CCGG 1 cut(s) 263
HphI GGTGA 4 cut(s) 101, 375, 832, 1096
Hpy166II GTNNAC 2 cut(s) 612, 1087
Hpy188I TCNGA 8 cut(s) 295, 312, 411, 494, 739, 809, 1135, 1265
Hpy188III TCNNGA 2 cut(s) 56, 572
Hpy8I GTNNAC 2 cut(s) 612, 1087
Hpy99I CGWCG 1 cut(s) 710
HpyAV CCTTC 2 cut(s) 45, 827
HpyCH4III ACNGT 4 cut(s) 107, 299, 898, 1009
HpyCH4IV ACGT 4 cut(s) 9, 173, 564, 1161
HpyF10VI GCNNNNNNNGC 3 cut(s) 356, 402, 1246
HpySE526I ACGT 4 cut(s) 9, 173, 564, 1161
Hsp92II CATG 6 cut(s) 522, 559, 582, 1028, 1041, 1201
HspAI GCGC 2 cut(s) 775, 992
KpnI GGTACC 1 cut(s) 141
Kzo9I GATC 3 cut(s) 351, 427, 1234
LguI GCTCTTC 1 cut(s) 264
LmnI GCTCC 2 cut(s) 28, 296
Lsp1109I GCAGC 3 cut(s) 980, 1084, 1104
LweI GCATC 2 cut(s) 387, 577
MaeI CTAG 3 cut(s) 63, 167, 1187
MaeII ACGT 4 cut(s) 9, 173, 564, 1161
MaeIII GTNAC 6 cut(s) 65, 107, 838, 856, 1003, 1139
MalI GATC 3 cut(s) 353, 429, 1236
MboI GATC 3 cut(s) 351, 427, 1234
MboII GAAGA 8 cut(s) 44, 134, 137, 195, 251, 544, 805, 1194
MfeI CAATTG 1 cut(s) 930
MhlI GDGCHC 4 cut(s) 330, 344, 375, 514
MluCI AATT 3 cut(s) 685, 930, 978
MlyI GAGTC 1 cut(s) 1157
MmeI TCCRAC 1 cut(s) 318
MnlI CCTC 8 cut(s) 19, 60, 167, 405, 428, 538, 1087, 1260
MroXI GAANNNNTTC 1 cut(s) 174
MseI TTAA 3 cut(s) 315, 480, 1031
MslI CAYNNNNRTG 3 cut(s) 251, 517, 583
MspI CCGG 1 cut(s) 263
MspR9I CCNGG 3 cut(s) 113, 229, 356
MunI CAATTG 1 cut(s) 930
Mva1269I GAATGC 1 cut(s) 405
MvaI CCWGG 3 cut(s) 113, 229, 356
MwoI GCNNNNNNNGC 3 cut(s) 356, 402, 1246
NdeII GATC 3 cut(s) 351, 427, 1234
NheI GCTAGC 1 cut(s) 1186
NlaIII CATG 6 cut(s) 522, 559, 582, 1028, 1041, 1201
NlaIV GGNNCC 4 cut(s) 30, 139, 416, 806
NmuCI GTSAC 5 cut(s) 107, 838, 856, 1003, 1139
NspI RCATGY 3 cut(s) 1028, 1041, 1201
NspV TTCGAA 1 cut(s) 457
PaeI GCATGC 1 cut(s) 1028
PciI ACATGT 1 cut(s) 1197
PciSI GCTCTTC 1 cut(s) 264
PcsI WCGNNNNNNNCGW 1 cut(s) 570
PctI GAATGC 1 cut(s) 405
PdmI GAANNNNTTC 1 cut(s) 174
PfeI GAWTC 5 cut(s) 59, 485, 575, 734, 810
PflMI CCANNNNNTGG 1 cut(s) 1020
PfoI TCCNGGA 1 cut(s) 227
PkrI GCNGC 6 cut(s) 361, 440, 911, 970, 1099, 1119
Ple19I CGATCG 1 cut(s) 430
PleI GAGTC 1 cut(s) 1156
PpsI GAGTC 1 cut(s) 1156
Ppu21I YACGTR 1 cut(s) 565
PscI ACATGT 1 cut(s) 1197
PsiI TTATAA 1 cut(s) 17
Psp124BI GAGCTC 1 cut(s) 330
Psp1406I AACGTT 1 cut(s) 173
Psp6I CCWGG 3 cut(s) 111, 227, 354
PspGI CCWGG 3 cut(s) 111, 227, 354
PspN4I GGNNCC 4 cut(s) 30, 139, 416, 806
PspPI GGNCC 1 cut(s) 148
PstI CTGCAG 1 cut(s) 828
PstNI CAGNNNCTG 1 cut(s) 1097
PvuI CGATCG 1 cut(s) 430
RsaI GTAC 3 cut(s) 139, 643, 1196
RsaNI GTAC 3 cut(s) 138, 642, 1195
RseI CAYNNNNRTG 3 cut(s) 251, 517, 583
SacI GAGCTC 1 cut(s) 330
SapI GCTCTTC 1 cut(s) 264
SaqAI TTAA 3 cut(s) 315, 480, 1031
SatI GCNGC 6 cut(s) 360, 439, 910, 969, 1098, 1118
Sau3AI GATC 3 cut(s) 351, 427, 1234
Sau96I GGNCC 1 cut(s) 148
SchI GAGTC 1 cut(s) 1157
ScrFI CCNGG 3 cut(s) 113, 229, 356
SduI GDGCHC 4 cut(s) 330, 344, 375, 514
SfaNI GCATC 2 cut(s) 387, 577
SfcI CTRYAG 1 cut(s) 824
SfuI TTCGAA 1 cut(s) 457
SinI GGWCC 1 cut(s) 148
SmiMI CAYNNNNRTG 3 cut(s) 251, 517, 583
SphI GCATGC 1 cut(s) 1028
Sse9I AATT 3 cut(s) 685, 930, 978
SsiI CCGC 8 cut(s) 5, 117, 359, 362, 438, 553, 843, 909
SspMI CTAG 3 cut(s) 63, 167, 1187
SstI GAGCTC 1 cut(s) 330
StyD4I CCNGG 3 cut(s) 111, 227, 354
TaaI ACNGT 4 cut(s) 107, 299, 898, 1009
TaiI ACGT 4 cut(s) 12, 176, 567, 1164
TaqI TCGA 6 cut(s) 57, 324, 426, 457, 705, 1151
TasI AATT 3 cut(s) 685, 930, 978
TatI WGTACW 1 cut(s) 641
TauI GCSGC 3 cut(s) 362, 441, 912
TfiI GAWTC 5 cut(s) 59, 485, 575, 734, 810
Tru1I TTAA 3 cut(s) 315, 480, 1031
Tru9I TTAA 3 cut(s) 315, 480, 1031
TscAI CASTG 4 cut(s) 519, 543, 901, 1251
TseFI GTSAC 5 cut(s) 107, 838, 856, 1003, 1139
TseI GCWGC 3 cut(s) 968, 1097, 1117
Tsp45I GTSAC 5 cut(s) 107, 838, 856, 1003, 1139
TspDTI ATGAA 2 cut(s) 567, 820
TspRI CASTG 4 cut(s) 519, 543, 901, 1251
Van91I CCANNNNNTGG 1 cut(s) 1020
VpaK11BI GGWCC 1 cut(s) 148
XagI CCTNNNNNAGG 1 cut(s) 1210
XapI RAATTY 1 cut(s) 978
XceI RCATGY 3 cut(s) 1028, 1041, 1201
XmnI GAANNNNTTC 1 cut(s) 174
XspI CTAG 3 cut(s) 63, 167, 1187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.