pycom16g10520

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0009383
Physical Location & Seq
Reverse (-)
7201702 .. 7202816
1115 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 837 bp
ATGTCAGGTGTGTCCCTTGCTGTGGCTCCTAGATCAGAGCCCGACACAACCACTACAGCCCCTGGATCCAAACCCCAAGACAAACCGCTCCACCGCCAACAGCACCAGCAGCCTGCTGTTGGCGGTGTAATGGGCTCATTGCGAGTAATAGAGCTCCAACTCGTGGCCTTCATCATGGTTTTCTCAGTCAGCGGCCTTGTCCCGCTTTTTGATTTGATCTTCCCGGCCTTTGCCTCAGCATATCTCTTGGCTCTGTCGCGCTTGGCCTTCCCTTCTCATGGTCCCGTCAGCTCTAGCTCTCGGGAGATTTTCCAAGGCAGCAGATTCTTCAGGTTTTACGTGGTTGCGGGAACCACAATCGGGCTCTTCTTGCCGCTTGCATACGTATTGGGTGGCTTTGCGCGGGGCGATGAGCACGCCGTCCGCTCGGCAACGCCTCACTTGTTCTTGCTCTCGTTTCAGATTCTCACTGAGAATGTGATTAGCGGGTTGTCGCTGTTTTCTCCGCCGGTGAGGGCATTGGTCCCATTGCTTTACACTGTCAGGAGAATCTTTGTCATCCTTGATTGGATGAAAGATGTTTGGCTTAACAAAACTCTGCCAGCCAATGCACAGTTTAAGGATGTTGCATGGTTTTGGTTTGGGAGGAGCCTGGCTGTAGCCAACTTCATATATTTCTCGATCAACCTATTCGGGTTTTTGATTCCTCGATTCCTTCCGAGGGCGTTTGAGAGATACTTCAAGGAGAGGGATGAACTTGAGTCCAAGAGTGTAGAGGACAAGCGCTCCGCAGCAGCAAATAAGTTGGAACCAGTTGCAGATAAGAAAAGTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

279

Amino Acids

30.99

Weight (kDa)

9.73

Isoelectric Point (pI)

50.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7733 PF24867 48 - 247 1.5e-80 Domain of unknown function (DUF7733)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 163
AccBSI CCGCTC 2 cut(s) 88, 426
AccII CGCG 2 cut(s) 259, 403
AclWI GGATC 2 cut(s) 60, 73
AcuI CTGAAG 1 cut(s) 313
AfeI AGCGCT 1 cut(s) 785
AfiI CCNNNNNNNGG 6 cut(s) 22, 119, 163, 278, 360, 721
AgsI TTSAA 1 cut(s) 742
AjnI CCWGG 2 cut(s) 61, 651
AluBI AGCT 3 cut(s) 154, 291, 297
AluI AGCT 3 cut(s) 154, 291, 297
Alw21I GWGCWC 2 cut(s) 156, 417
AlwI GGATC 2 cut(s) 60, 73
AlwNI CAGNNNCTG 1 cut(s) 62
Ama87I CYCGRG 1 cut(s) 300
Aor51HI AGCGCT 1 cut(s) 785
AoxI GGCC 4 cut(s) 165, 193, 225, 264
ApeKI GCWGC 4 cut(s) 109, 318, 791, 794
AspLEI GCGC 3 cut(s) 261, 403, 786
AspS9I GGNCC 2 cut(s) 281, 523
AsuC2I CCSGG 1 cut(s) 224
AsuHPI GGTGA 1 cut(s) 523
AvaI CYCGRG 1 cut(s) 300
AvaII GGWCC 2 cut(s) 281, 523
BamHI GGATCC 1 cut(s) 65
BanII GRGCYC 4 cut(s) 42, 137, 156, 366
BauI CACGAG 1 cut(s) 161
Bbv12I GWGCWC 2 cut(s) 156, 417
BbvCI CCTCAGC 1 cut(s) 235
BbvI GCAGC 4 cut(s) 121, 330, 803, 806
BceAI ACGGC 1 cut(s) 404
BciT130I CCWGG 2 cut(s) 63, 653
BcnI CCSGG 1 cut(s) 224
BfaI CTAG 2 cut(s) 30, 294
BfmI CTRYAG 2 cut(s) 54, 657
BfoI RGCGCY 1 cut(s) 787
BisI GCNGC 6 cut(s) 110, 193, 319, 374, 792, 795
BlsI GCNGC 6 cut(s) 111, 194, 320, 375, 793, 796
Bme1390I CCNGG 3 cut(s) 63, 224, 653
Bme18I GGWCC 2 cut(s) 281, 523
BmeT110I CYCGRG 1 cut(s) 300
BmgT120I GGNCC 2 cut(s) 281, 523
BmiI GGNNCC 7 cut(s) 27, 67, 283, 352, 525, 650, 810
BmrFI CCNGG 3 cut(s) 63, 224, 653
Bpu10I CCTNAGC 1 cut(s) 235
BpuEI CTTGAG 1 cut(s) 779
BpuMI CCSGG 1 cut(s) 224
BsaAI YACGTR 2 cut(s) 340, 385
BsaJI CCNNGG 3 cut(s) 61, 313, 719
BsaXI ACNNNNNCTCC 2 cut(s) 770, 800
Bsc4I CCNNNNNNNGG 6 cut(s) 22, 119, 163, 278, 360, 721
Bse118I RCCGGY 1 cut(s) 508
Bse1I ACTGG 1 cut(s) 812
Bse3DI GCAATG 2 cut(s) 137, 527
BseBI CCWGG 2 cut(s) 63, 653
BseDI CCNNGG 3 cut(s) 61, 313, 719
BseGI GGATG 4 cut(s) 558, 576, 628, 757
BseLI CCNNNNNNNGG 6 cut(s) 22, 119, 163, 278, 360, 721
BseMI GCAATG 2 cut(s) 137, 527
BseMII CTCAG 3 cut(s) 198, 249, 462
BseNI ACTGG 1 cut(s) 812
BseRI GAGGAG 1 cut(s) 661
BseXI GCAGC 4 cut(s) 121, 330, 803, 806
Bsh1236I CGCG 2 cut(s) 259, 403
BshFI GGCC 4 cut(s) 167, 195, 227, 266
BsiHKAI GWGCWC 2 cut(s) 156, 417
BsiHKCI CYCGRG 1 cut(s) 300
BsiSI CCGG 2 cut(s) 224, 509
BslFI GGGAC 3 cut(s) 185, 267, 509
BslI CCNNNNNNNGG 6 cut(s) 22, 119, 163, 278, 360, 721
BsmFI GGGAC 3 cut(s) 185, 267, 509
BsnI GGCC 4 cut(s) 167, 195, 227, 266
BsoBI CYCGRG 1 cut(s) 300
Bsp1286I GDGCHC 5 cut(s) 42, 137, 156, 366, 417
Bsp143I GATC 4 cut(s) 32, 65, 216, 681
BspANI GGCC 4 cut(s) 167, 195, 227, 266
BspCNI CTCAG 3 cut(s) 197, 248, 463
BspFNI CGCG 2 cut(s) 259, 403
BspLI GGNNCC 7 cut(s) 27, 67, 283, 352, 525, 650, 810
BspPI GGATC 2 cut(s) 60, 73
BspQI GCTCTTC 1 cut(s) 371
BsrBI CCGCTC 2 cut(s) 88, 426
BsrDI GCAATG 2 cut(s) 137, 527
BsrFI RCCGGY 1 cut(s) 508
BsrI ACTGG 1 cut(s) 812
BssAI RCCGGY 1 cut(s) 508
BssECI CCNNGG 3 cut(s) 61, 313, 719
BssMI GATC 4 cut(s) 32, 65, 216, 681
BssSI CACGAG 1 cut(s) 161
BssT1I CCWWGG 1 cut(s) 313
Bst2BI CACGAG 1 cut(s) 161
Bst2UI CCWGG 2 cut(s) 63, 653
Bst4CI ACNGT 2 cut(s) 541, 615
Bst6I CTCTTC 1 cut(s) 371
BstBAI YACGTR 2 cut(s) 340, 385
BstC8I GCNNGC 4 cut(s) 114, 378, 417, 603
BstDEI CTNAG 3 cut(s) 184, 235, 471
BstF5I GGATG 4 cut(s) 558, 576, 628, 757
BstFNI CGCG 2 cut(s) 259, 403
BstH2I RGCGCY 1 cut(s) 787
BstHHI GCGC 3 cut(s) 261, 403, 786
BstKTI GATC 4 cut(s) 35, 68, 219, 684
BstMBI GATC 4 cut(s) 32, 65, 216, 681
BstMWI GCNNNNNNNGC 2 cut(s) 109, 370
BstNI CCWGG 2 cut(s) 63, 653
BstSCI CCNGG 3 cut(s) 61, 222, 651
BstSFI CTRYAG 2 cut(s) 54, 657
BstSNI TACGTA 1 cut(s) 385
BstUI CGCG 2 cut(s) 259, 403
BstV1I GCAGC 4 cut(s) 121, 330, 803, 806
BstX2I RGATCY 1 cut(s) 65
BstYI RGATCY 1 cut(s) 65
BsuRI GGCC 4 cut(s) 167, 195, 227, 266
BtgZI GCGATG 1 cut(s) 423
BtsCI GGATG 4 cut(s) 558, 576, 628, 757
BtsIMutI CAGTG 2 cut(s) 468, 537
Cac8I GCNNGC 4 cut(s) 114, 378, 417, 603
CaiI CAGNNNCTG 1 cut(s) 62
CfoI GCGC 3 cut(s) 261, 403, 786
Cfr10I RCCGGY 1 cut(s) 508
Cfr13I GGNCC 2 cut(s) 281, 523
CviAII CATG 3 cut(s) 175, 278, 630
DdeI CTNAG 3 cut(s) 184, 235, 471
DpnI GATC 4 cut(s) 34, 67, 218, 683
DpnII GATC 4 cut(s) 32, 65, 216, 681
Eam1104I CTCTTC 1 cut(s) 371
EarI CTCTTC 1 cut(s) 371
EciI GGCGGA 1 cut(s) 495
Ecl136II GAGCTC 1 cut(s) 154
Eco105I TACGTA 1 cut(s) 385
Eco130I CCWWGG 1 cut(s) 313
Eco24I GRGCYC 4 cut(s) 42, 137, 156, 366
Eco47I GGWCC 2 cut(s) 281, 523
Eco47III AGCGCT 1 cut(s) 785
Eco53kI GAGCTC 1 cut(s) 154
Eco57I CTGAAG 1 cut(s) 313
Eco88I CYCGRG 1 cut(s) 300
EcoICRI GAGCTC 1 cut(s) 154
EcoRII CCWGG 2 cut(s) 61, 651
EcoT14I CCWWGG 1 cut(s) 313
EcoT38I GRGCYC 4 cut(s) 42, 137, 156, 366
ErhI CCWWGG 1 cut(s) 313
FaeI CATG 3 cut(s) 178, 281, 633
FaiI YATR 7 cut(s) 176, 241, 279, 382, 631, 671, 673
FaqI GGGAC 3 cut(s) 185, 267, 509
FatI CATG 3 cut(s) 174, 277, 629
FauI CCCGC 4 cut(s) 210, 340, 396, 479
Fnu4HI GCNGC 6 cut(s) 110, 193, 319, 374, 792, 795
FokI GGATG 4 cut(s) 545, 583, 635, 764
FriOI GRGCYC 4 cut(s) 42, 137, 156, 366
Fsp4HI GCNGC 6 cut(s) 110, 193, 319, 374, 792, 795
FspBI CTAG 2 cut(s) 30, 294
GlaI GCGC 3 cut(s) 260, 402, 785
GluI GCNGC 6 cut(s) 110, 193, 319, 374, 792, 795
HaeII RGCGCY 1 cut(s) 787
HaeIII GGCC 4 cut(s) 167, 195, 227, 266
HapII CCGG 2 cut(s) 224, 509
HhaI GCGC 3 cut(s) 261, 403, 786
Hin1II CATG 3 cut(s) 178, 281, 633
Hin6I GCGC 3 cut(s) 259, 401, 784
HinP1I GCGC 3 cut(s) 259, 401, 784
HinfI GANTC 6 cut(s) 324, 463, 549, 703, 711, 761
HpaII CCGG 2 cut(s) 224, 509
HphI GGTGA 1 cut(s) 523
Hpy188I TCNGA 3 cut(s) 37, 462, 720
Hpy188III TCNNGA 3 cut(s) 302, 544, 679
HpyAV CCTTC 4 cut(s) 178, 277, 282, 725
HpyCH4III ACNGT 2 cut(s) 541, 615
HpyCH4IV ACGT 2 cut(s) 339, 384
HpyCH4V TGCA 4 cut(s) 380, 611, 629, 818
HpyF10VI GCNNNNNNNGC 2 cut(s) 109, 370
HpyF3I CTNAG 3 cut(s) 184, 235, 471
HpySE526I ACGT 2 cut(s) 339, 384
Hsp92II CATG 3 cut(s) 178, 281, 633
HspAI GCGC 3 cut(s) 259, 401, 784
Kzo9I GATC 4 cut(s) 32, 65, 216, 681
LguI GCTCTTC 1 cut(s) 371
LmnI GCTCC 5 cut(s) 31, 93, 159, 648, 791
Lsp1109I GCAGC 4 cut(s) 121, 330, 803, 806
MaeI CTAG 2 cut(s) 30, 294
MaeII ACGT 2 cut(s) 339, 384
MalI GATC 4 cut(s) 34, 67, 218, 683
MbiI CCGCTC 2 cut(s) 88, 426
MboI GATC 4 cut(s) 32, 65, 216, 681
MboII GAAGA 3 cut(s) 211, 319, 358
MflI RGATCY 1 cut(s) 65
MhlI GDGCHC 5 cut(s) 42, 137, 156, 366, 417
MlyI GAGTC 1 cut(s) 770
MmeI TCCRAC 2 cut(s) 181, 786
MnlI CCTC 8 cut(s) 244, 447, 507, 639, 714, 717, 741, 769
MseI TTAA 2 cut(s) 588, 618
MspA1I CMGCKG 1 cut(s) 192
MspI CCGG 2 cut(s) 224, 509
MspR9I CCNGG 3 cut(s) 63, 224, 653
MvaI CCWGG 2 cut(s) 63, 653
MvnI CGCG 2 cut(s) 259, 403
MwoI GCNNNNNNNGC 2 cut(s) 109, 370
NciI CCSGG 1 cut(s) 224
NdeII GATC 4 cut(s) 32, 65, 216, 681
NlaIII CATG 3 cut(s) 178, 281, 633
NlaIV GGNNCC 7 cut(s) 27, 67, 283, 352, 525, 650, 810
NmeAIII GCCGAG 1 cut(s) 407
PciSI GCTCTTC 1 cut(s) 371
PfeI GAWTC 5 cut(s) 324, 463, 549, 703, 711
PflMI CCANNNNNTGG 1 cut(s) 163
PkrI GCNGC 6 cut(s) 111, 194, 320, 375, 793, 796
PleI GAGTC 1 cut(s) 769
PpsI GAGTC 1 cut(s) 769
Ppu21I YACGTR 2 cut(s) 340, 385
Psp124BI GAGCTC 1 cut(s) 156
Psp6I CCWGG 2 cut(s) 61, 651
PspGI CCWGG 2 cut(s) 61, 651
PspN4I GGNNCC 7 cut(s) 27, 67, 283, 352, 525, 650, 810
PspPI GGNCC 2 cut(s) 281, 523
PstNI CAGNNNCTG 1 cut(s) 62
PsuI RGATCY 1 cut(s) 65
SacI GAGCTC 1 cut(s) 156
SapI GCTCTTC 1 cut(s) 371
SaqAI TTAA 2 cut(s) 588, 618
SatI GCNGC 6 cut(s) 110, 193, 319, 374, 792, 795
Sau3AI GATC 4 cut(s) 32, 65, 216, 681
Sau96I GGNCC 2 cut(s) 281, 523
SchI GAGTC 1 cut(s) 770
ScrFI CCNGG 3 cut(s) 63, 224, 653
SduI GDGCHC 5 cut(s) 42, 137, 156, 366, 417
SetI ASST 8 cut(s) 10, 156, 293, 299, 335, 342, 387, 690
SfcI CTRYAG 2 cut(s) 54, 657
SgrAI CRCCGGYG 1 cut(s) 508
SinI GGWCC 2 cut(s) 281, 523
SmlI CTYRAG 1 cut(s) 758
SmoI CTYRAG 1 cut(s) 758
SnaBI TACGTA 1 cut(s) 385
SspMI CTAG 2 cut(s) 30, 294
SstI GAGCTC 1 cut(s) 156
StyD4I CCNGG 3 cut(s) 61, 222, 651
StyI CCWWGG 1 cut(s) 313
TaaI ACNGT 2 cut(s) 541, 615
TaiI ACGT 2 cut(s) 342, 387
TaqI TCGA 2 cut(s) 680, 709
TauI GCSGC 2 cut(s) 195, 376
TfiI GAWTC 5 cut(s) 324, 463, 549, 703, 711
Tru1I TTAA 2 cut(s) 588, 618
Tru9I TTAA 2 cut(s) 588, 618
TscAI CASTG 2 cut(s) 475, 544
TseI GCWGC 4 cut(s) 109, 318, 791, 794
TspDTI ATGAA 4 cut(s) 160, 587, 658, 768
TspRI CASTG 2 cut(s) 475, 544
Van91I CCANNNNNTGG 1 cut(s) 163
VpaK11BI GGWCC 2 cut(s) 281, 523
XspI CTAG 2 cut(s) 30, 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.