pycom16g10600

GYF domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0005720
Physical Location & Seq
Reverse (-)
7260110 .. 7261475
1366 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1272 bp
ATGTCCCTCCTGAGGATTTATGTTTGTATTACCTTGATCGATCGTCAATCTCAAGGGGCGGTTCAGGGACCATATTTGGGGGTAGACATTATTTCATGGTTTGAACAAGGGTTTTTTGGGACAGACTTGCCTGTTCGATTGGCGGATACTCCCGAAGGAACACCTTTCATGGAATTGGGGGAGGTCATGCCGCATCTGAAAGTTTGGGATGCGCAAGGGAATTTTGTAAATCCAACTTCTCATATAGAAGAATCAAGTGGCTCCTTAGGGAATATAGAGTCTAGCTTACCTTCTTCCGCTCCAGTTTCAGAAATGACTACATTAGTGGTGAATGACTTACGGCGGCCATTGACTGATCTAAATAGCCTTTCAACTCTGCATATTCAACCTAGAAATTCTGATCCTGAAGCTCCACTACAACTGCCACATTCAACGGCTCAAAGCTTTAATGATTTTTGTGCAGAAGATGAAGATATTGTGTTTCCAGGAATACCTGGAACCACCGGCTATTCTACTGCAAGATCTTCTGGAACCATTCATGATTCTATTGAAAATTCTATTGGCCACCTTCCTTCTCCAACTGAGCTGACAGAATCTGGTGTGCCTATTCAGAATGATGACAAGTTGCACCCCTTTGGCTTGCTTTGGTCTGAGTTAGAAGGCGGTCAAACAAAACAAGTCAAGCCAGCCAATAGGCTTTCGAACATGGGGAGGGCTGTTCCATTTAGTGGAATTTCTGATACAGCGATTCTTGCTGAGACATGGTCTGATGTTCATAGAAAAAATGCAGTCTCTGATTCGAACCTGTATCAAGACATGGTAGCCCCTCGTCAATTGGCACACATGGAACAGGAACCCAGTCATTATGATTTAGCGGAGCAGCTTATGTCACAGCAAATTCGACAGCAGCAGCTCCAACAGCGGAATATGTTGTCTAGCTTTGGACACTTGAATGATGCAGCATTAGAGCATTTGCCAAGTCAGAATCTTATCCACCAACAGTTAGCCAACCATTCTTCAGCAGATCTGGATCATCTTGTGGCACTTAAGATGCAACAGCACCGGGAGGCTCAGCTCCAGCAACATCAGCAACTGCAACAGCAGCAGTTTCATCAACAGCAGAAGCTTTTACAGGAGCAACAGCAGTCCCAAGTGCAACGGGTGCTTCTTGAACAACTACTGCATGGTCAAATGCATGATCGATCCCTGCGCTTAGGCAGCCACACGTTGATCCTGTCAGAGCAAACAACGTTCTTGAACAGGTTCTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF

Protein Analysis

424

Amino Acids

47.39

Weight (kDa)

5.22

Isoelectric Point (pI)

66.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GYF PF02213 16 - 56 2.6e-08 GYF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 213
AccB7I CCANNNNNTGG 1 cut(s) 728
AccBSI CCGCTC 1 cut(s) 299
AccI GTMKAC 1 cut(s) 84
AciI CCGC 8 cut(s) 59, 143, 191, 297, 343, 663, 875, 922
AclI AACGTT 1 cut(s) 1250
AclWI GGATC 4 cut(s) 395, 1038, 1197, 1225
AcoI YGGCCR 2 cut(s) 344, 562
AcsI RAATTY 5 cut(s) 220, 394, 553, 732, 897
AcuI CTGAAG 2 cut(s) 426, 1002
AfiI CCNNNNNNNGG 3 cut(s) 12, 77, 728
AflII CTTAAG 1 cut(s) 1046
AflIII ACRYGT 1 cut(s) 1224
AgsI TTSAA 8 cut(s) 104, 372, 386, 432, 551, 952, 1172, 1258
AjnI CCWGG 2 cut(s) 484, 493
AjuI GAANNNNNNNTTGG 2 cut(s) 543, 575
AluBI AGCT 9 cut(s) 285, 410, 444, 586, 883, 913, 939, 1075, 1126
AluI AGCT 9 cut(s) 285, 410, 444, 586, 883, 913, 939, 1075, 1126
Alw26I GTCTC 2 cut(s) 752, 796
AlwI GGATC 4 cut(s) 395, 1038, 1197, 1225
AlwNI CAGNNNCTG 3 cut(s) 596, 794, 1093
AoxI GGCC 2 cut(s) 344, 562
ApeKI GCWGC 6 cut(s) 880, 907, 910, 959, 1102, 1218
ApoI RAATTY 5 cut(s) 220, 394, 553, 732, 897
Asp700I GAANNNNTTC 1 cut(s) 1262
AspLEI GCGC 2 cut(s) 214, 1212
AspS9I GGNCC 1 cut(s) 68
AsuC2I CCSGG 1 cut(s) 1064
AsuHPI GGTGA 1 cut(s) 340
AsuII TTCGAA 2 cut(s) 701, 800
AvaII GGWCC 1 cut(s) 68
AxyI CCTNAGG 2 cut(s) 11, 265
BalI TGGCCA 1 cut(s) 564
BarI GAAGNNNNNNTAC 2 cut(s) 399, 431
BbvI GCAGC 6 cut(s) 892, 919, 922, 971, 1114, 1230
BceAI ACGGC 2 cut(s) 356, 450
BciT130I CCWGG 2 cut(s) 486, 495
BciVI GTATCC 1 cut(s) 139
BcnI CCSGG 1 cut(s) 1064
BcoDI GTCTC 2 cut(s) 752, 796
BfaI CTAG 3 cut(s) 282, 390, 936
BfrI CTTAAG 1 cut(s) 1046
BfuI GTATCC 1 cut(s) 139
BglII AGATCT 2 cut(s) 521, 1024
BisI GCNGC 8 cut(s) 191, 344, 881, 908, 911, 960, 1103, 1219
BlpI GCTNAGC 1 cut(s) 1071
BlsI GCNGC 8 cut(s) 192, 345, 882, 909, 912, 961, 1104, 1220
Bme1390I CCNGG 3 cut(s) 486, 495, 1064
Bme18I GGWCC 1 cut(s) 68
BmgT120I GGNCC 1 cut(s) 68
BmiI GGNNCC 5 cut(s) 69, 262, 499, 532, 855
BmrFI CCNGG 3 cut(s) 486, 495, 1064
BmrI ACTGGG 1 cut(s) 852
BmsI GCATC 4 cut(s) 199, 202, 946, 1041
BmuI ACTGGG 1 cut(s) 852
BpmI CTGGAG 2 cut(s) 285, 1061
Bpu10I CCTNAGC 1 cut(s) 1213
Bpu1102I GCTNAGC 1 cut(s) 1071
Bpu14I TTCGAA 2 cut(s) 701, 800
BpuEI CTTGAG 1 cut(s) 36
BpuMI CCSGG 1 cut(s) 1064
Bsa29I ATCGAT 2 cut(s) 39, 1201
BsaBI GATNNNNATC 1 cut(s) 1029
Bsc4I CCNNNNNNNGG 3 cut(s) 12, 77, 728
Bse118I RCCGGY 1 cut(s) 503
Bse1I ACTGG 2 cut(s) 302, 858
Bse21I CCTNAGG 2 cut(s) 11, 265
Bse8I GATNNNNATC 1 cut(s) 1029
BseBI CCWGG 2 cut(s) 486, 495
BseCI ATCGAT 2 cut(s) 39, 1201
BseGI GGATG 1 cut(s) 214
BseJI GATNNNNATC 1 cut(s) 1029
BseLI CCNNNNNNNGG 3 cut(s) 12, 77, 728
BseMII CTCAG 4 cut(s) 573, 642, 747, 1085
BseNI ACTGG 2 cut(s) 302, 858
BseXI GCAGC 6 cut(s) 892, 919, 922, 971, 1114, 1230
BsgI GTGCAG 1 cut(s) 480
Bsh1285I CGRYCG 1 cut(s) 43
BshFI GGCC 2 cut(s) 346, 564
BshVI ATCGAT 2 cut(s) 39, 1201
BsiEI CGRYCG 1 cut(s) 43
BsiSI CCGG 2 cut(s) 504, 1063
BslFI GGGAC 3 cut(s) 81, 133, 1132
BslI CCNNNNNNNGG 3 cut(s) 12, 77, 728
BsmAI GTCTC 2 cut(s) 752, 796
BsmFI GGGAC 3 cut(s) 81, 133, 1132
BsnI GGCC 2 cut(s) 346, 564
Bsp119I TTCGAA 2 cut(s) 701, 800
Bsp1720I GCTNAGC 1 cut(s) 1071
BspACI CCGC 8 cut(s) 59, 143, 191, 297, 343, 663, 875, 922
BspANI GGCC 2 cut(s) 346, 564
BspCNI CTCAG 4 cut(s) 574, 643, 748, 1084
BspDI ATCGAT 2 cut(s) 39, 1201
BspHI TCATGA 1 cut(s) 538
BspLI GGNNCC 5 cut(s) 69, 262, 499, 532, 855
BspPI GGATC 4 cut(s) 395, 1038, 1197, 1225
BspT104I TTCGAA 2 cut(s) 701, 800
BspTI CTTAAG 1 cut(s) 1046
BsrBI CCGCTC 1 cut(s) 299
BsrFI RCCGGY 1 cut(s) 503
BsrI ACTGG 2 cut(s) 302, 858
BssAI RCCGGY 1 cut(s) 503
Bst2UI CCWGG 2 cut(s) 486, 495
Bst4CI ACNGT 1 cut(s) 1002
BstAFI CTTAAG 1 cut(s) 1046
BstAPI GCANNNNNTGC 1 cut(s) 1162
BstBI TTCGAA 2 cut(s) 701, 800
BstC8I GCNNGC 2 cut(s) 641, 687
BstDEI CTNAG 7 cut(s) 11, 265, 582, 651, 756, 1071, 1213
BstF5I GGATG 1 cut(s) 214
BstHHI GCGC 2 cut(s) 214, 1212
BstMAI GTCTC 2 cut(s) 752, 796
BstMCI CGRYCG 1 cut(s) 43
BstMWI GCNNNNNNNGC 6 cut(s) 752, 919, 1087, 1102, 1162, 1218
BstNI CCWGG 2 cut(s) 486, 495
BstSCI CCNGG 3 cut(s) 484, 493, 1062
BstV1I GCAGC 6 cut(s) 892, 919, 922, 971, 1114, 1230
BstX2I RGATCY 2 cut(s) 521, 1024
BstYI RGATCY 2 cut(s) 521, 1024
Bsu15I ATCGAT 2 cut(s) 39, 1201
Bsu36I CCTNAGG 2 cut(s) 11, 265
BsuI GTATCC 1 cut(s) 139
BsuRI GGCC 2 cut(s) 346, 564
BsuTUI ATCGAT 2 cut(s) 39, 1201
BtsCI GGATG 1 cut(s) 214
Cac8I GCNNGC 2 cut(s) 641, 687
CaiI CAGNNNCTG 3 cut(s) 596, 794, 1093
CciI TCATGA 1 cut(s) 538
CfoI GCGC 2 cut(s) 214, 1212
Cfr10I RCCGGY 1 cut(s) 503
Cfr13I GGNCC 1 cut(s) 68
ClaI ATCGAT 2 cut(s) 39, 1201
DdeI CTNAG 7 cut(s) 11, 265, 582, 651, 756, 1071, 1213
EaeI YGGCCR 2 cut(s) 344, 562
EciI GGCGGA 1 cut(s) 158
Eco47I GGWCC 1 cut(s) 68
Eco57I CTGAAG 2 cut(s) 426, 1002
Eco81I CCTNAGG 2 cut(s) 11, 265
EcoRII CCWGG 2 cut(s) 484, 493
EcoT22I ATGCAT 1 cut(s) 1197
FaqI GGGAC 3 cut(s) 81, 133, 1132
FblI GTMKAC 1 cut(s) 84
Fnu4HI GCNGC 8 cut(s) 191, 344, 881, 908, 911, 960, 1103, 1219
FokI GGATG 1 cut(s) 221
Fsp4HI GCNGC 8 cut(s) 191, 344, 881, 908, 911, 960, 1103, 1219
FspBI CTAG 3 cut(s) 282, 390, 936
FspI TGCGCA 1 cut(s) 213
GlaI GCGC 2 cut(s) 213, 1211
GluI GCNGC 8 cut(s) 191, 344, 881, 908, 911, 960, 1103, 1219
GsuI CTGGAG 2 cut(s) 285, 1061
HaeIII GGCC 2 cut(s) 346, 564
HapII CCGG 2 cut(s) 504, 1063
HhaI GCGC 2 cut(s) 214, 1212
Hin6I GCGC 2 cut(s) 212, 1210
HinP1I GCGC 2 cut(s) 212, 1210
HindIII AAGCTT 2 cut(s) 442, 1124
HinfI GANTC 7 cut(s) 251, 278, 542, 593, 748, 797, 985
HpaII CCGG 2 cut(s) 504, 1063
HphI GGTGA 1 cut(s) 340
Hpy166II GTNNAC 1 cut(s) 85
Hpy188III TCNNGA 9 cut(s) 10, 152, 404, 528, 539, 812, 1028, 1169, 1255
Hpy8I GTNNAC 1 cut(s) 85
HpyAV CCTTC 5 cut(s) 149, 300, 578, 582, 653
HpyCH4III ACNGT 1 cut(s) 1002
HpyCH4IV ACGT 2 cut(s) 1226, 1250
HpyF10VI GCNNNNNNNGC 6 cut(s) 752, 919, 1087, 1102, 1162, 1218
HpyF3I CTNAG 7 cut(s) 11, 265, 582, 651, 756, 1071, 1213
HpySE526I ACGT 2 cut(s) 1226, 1250
HspAI GCGC 2 cut(s) 212, 1210
LmnI GCTCC 7 cut(s) 266, 304, 415, 877, 918, 1080, 1135
Lsp1109I GCAGC 6 cut(s) 892, 919, 922, 971, 1114, 1230
LweI GCATC 4 cut(s) 199, 202, 946, 1041
MaeI CTAG 3 cut(s) 282, 390, 936
MaeII ACGT 2 cut(s) 1226, 1250
MaeIII GTNAC 1 cut(s) 888
MbiI CCGCTC 1 cut(s) 299
MboII GAAGA 6 cut(s) 260, 285, 476, 482, 516, 1008
MfeI CAATTG 1 cut(s) 833
MflI RGATCY 2 cut(s) 521, 1024
MlsI TGGCCA 1 cut(s) 564
MluCI AATT 7 cut(s) 173, 220, 394, 553, 732, 833, 897
MluNI TGGCCA 1 cut(s) 564
MlyI GAGTC 1 cut(s) 287
MmeI TCCRAC 3 cut(s) 257, 602, 940
MnlI CCTC 6 cut(s) 6, 17, 175, 705, 837, 1060
Mox20I TGGCCA 1 cut(s) 564
Mph1103I ATGCAT 1 cut(s) 1197
MroXI GAANNNNTTC 1 cut(s) 1262
MscI TGGCCA 1 cut(s) 564
MseI TTAA 2 cut(s) 447, 1047
MslI CAYNNNNRTG 1 cut(s) 951
Msp20I TGGCCA 1 cut(s) 564
MspA1I CMGCKG 1 cut(s) 922
MspCI CTTAAG 1 cut(s) 1046
MspI CCGG 2 cut(s) 504, 1063
MspR9I CCNGG 3 cut(s) 486, 495, 1064
MunI CAATTG 1 cut(s) 833
MvaI CCWGG 2 cut(s) 486, 495
MwoI GCNNNNNNNGC 6 cut(s) 752, 919, 1087, 1102, 1162, 1218
NciI CCSGG 1 cut(s) 1064
NlaIV GGNNCC 5 cut(s) 69, 262, 499, 532, 855
NmuCI GTSAC 1 cut(s) 888
NsbI TGCGCA 1 cut(s) 213
NsiI ATGCAT 1 cut(s) 1197
NspV TTCGAA 2 cut(s) 701, 800
PagI TCATGA 1 cut(s) 538
PdmI GAANNNNTTC 1 cut(s) 1262
PfeI GAWTC 6 cut(s) 251, 542, 593, 748, 797, 985
PflFI GACNNNGTC 1 cut(s) 763
PflMI CCANNNNNTGG 1 cut(s) 728
PfoI TCCNGGA 1 cut(s) 484
PkrI GCNGC 8 cut(s) 192, 345, 882, 909, 912, 961, 1104, 1220
Ple19I CGATCG 1 cut(s) 43
PleI GAGTC 1 cut(s) 286
PpsI GAGTC 1 cut(s) 286
Psp1406I AACGTT 1 cut(s) 1250
Psp6I CCWGG 2 cut(s) 484, 493
PspGI CCWGG 2 cut(s) 484, 493
PspN4I GGNNCC 5 cut(s) 69, 262, 499, 532, 855
PspPI GGNCC 1 cut(s) 68
PstNI CAGNNNCTG 3 cut(s) 596, 794, 1093
PsuI RGATCY 2 cut(s) 521, 1024
PsyI GACNNNGTC 1 cut(s) 763
PvuI CGATCG 1 cut(s) 43
RseI CAYNNNNRTG 1 cut(s) 951
SaqAI TTAA 2 cut(s) 447, 1047
SatI GCNGC 8 cut(s) 191, 344, 881, 908, 911, 960, 1103, 1219
Sau96I GGNCC 1 cut(s) 68
SchI GAGTC 1 cut(s) 287
ScrFI CCNGG 3 cut(s) 486, 495, 1064
SfaNI GCATC 4 cut(s) 199, 202, 946, 1041
SfuI TTCGAA 2 cut(s) 701, 800
SinI GGWCC 1 cut(s) 68
SmiMI CAYNNNNRTG 1 cut(s) 951
SmlI CTYRAG 2 cut(s) 51, 1046
SmoI CTYRAG 2 cut(s) 51, 1046
Sse9I AATT 7 cut(s) 173, 220, 394, 553, 732, 833, 897
SsiI CCGC 8 cut(s) 59, 143, 191, 297, 343, 663, 875, 922
SspMI CTAG 3 cut(s) 282, 390, 936
StyD4I CCNGG 3 cut(s) 484, 493, 1062
TaaI ACNGT 1 cut(s) 1002
TaiI ACGT 2 cut(s) 1229, 1253
TaqI TCGA 6 cut(s) 39, 136, 701, 800, 901, 1201
TasI AATT 7 cut(s) 173, 220, 394, 553, 732, 833, 897
TauI GCSGC 2 cut(s) 193, 346
TfiI GAWTC 6 cut(s) 251, 542, 593, 748, 797, 985
Tru1I TTAA 2 cut(s) 447, 1047
Tru9I TTAA 2 cut(s) 447, 1047
TseFI GTSAC 1 cut(s) 888
TseI GCWGC 6 cut(s) 880, 907, 910, 959, 1102, 1218
Tsp45I GTSAC 1 cut(s) 888
TspDTI ATGAA 6 cut(s) 84, 157, 483, 527, 764, 1100
Tth111I GACNNNGTC 1 cut(s) 763
Van91I CCANNNNNTGG 1 cut(s) 728
Vha464I CTTAAG 1 cut(s) 1046
VpaK11BI GGWCC 1 cut(s) 68
XapI RAATTY 5 cut(s) 220, 394, 553, 732, 897
XmiI GTMKAC 1 cut(s) 84
XmnI GAANNNNTTC 1 cut(s) 1262
XspI CTAG 3 cut(s) 282, 390, 936
Zsp2I ATGCAT 1 cut(s) 1197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.