pycom16g12980

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0008465
Physical Location & Seq
Reverse (-)
9213172 .. 9215153
1982 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 717 bp
ATGCTTCAGGCATTGATGAATCTGCTCTCTCTTTGCTGGAGGCCATTTGGGCGCGGCGACGGTGAGGGACTCAGCTCTGTTGGAATCGTCGGCGCTGCGAATAACGGAGGCGGCGGAGGAGGACGGGATGGCAAAGATAACTTGCTTTGGTTCCGAGATTTTGGAAAGTACGCGTGGGGCGATTTCTCTATGGCGGTGGTCCAGGCCAATCAGGTGCTCGAGGATCAGAGCCAGATCGAGTCTGGACCCTTTGGGACCTTCGTCGGCGTATACGACGGACACGGCGGCCCGGAGACCGCTCGATACGTTTGCGACAATTTATTCAGACATTTCCAAGCAATATCAGCGGAGTCTGAAGGTGTTGTGACAGCAGAGACCATTCAAAATGCTTTTCTCCGAACAGAGGAAGGGTTTACCGCCCTTGTTTCGGAGCTGTGGGGTAATAAACCCCAAGTAGCAACTGTTGGGTCATGCTGTCTGGTTGGAGTTATATGTCAGGGGACCCTATTTGTGGCAAACCTGGGAGATTCCCGTTGTGTGTTGGGAAAGAAAGTGGGCAACACTGGAGAAGTTGCTGCGATCCAGTTATCCACTGAACACAATGCAAATCTCGAGGAGGTTAGGCATGAGCTGAAAGATTTGCACCCACATCGATCCTCAAATTGTCGTTTTAAAGCATGGAGTTTGGAGAGTAAAAGGCATTATCCAGGTATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

239

Amino Acids

25.68

Weight (kDa)

5.64

Isoelectric Point (pI)

39.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 86 - 206 1.9e-12 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 299
AccI GTMKAC 1 cut(s) 270
AccII CGCG 2 cut(s) 54, 173
AciI CCGC 8 cut(s) 54, 111, 114, 194, 285, 297, 347, 417
AclWI GGATC 3 cut(s) 231, 574, 648
AcuI CTGAAG 1 cut(s) 375
AfaI GTAC 1 cut(s) 170
AfiI CCNNNNNNNGG 3 cut(s) 403, 427, 511
AflIII ACRYGT 1 cut(s) 171
AgsI TTSAA 1 cut(s) 383
AjnI CCWGG 3 cut(s) 201, 519, 706
AluBI AGCT 3 cut(s) 75, 433, 631
AluI AGCT 3 cut(s) 75, 433, 631
Alw21I GWGCWC 1 cut(s) 219
Alw26I GTCTC 2 cut(s) 287, 368
AlwI GGATC 3 cut(s) 231, 574, 648
Ama87I CYCGRG 2 cut(s) 218, 611
AoxI GGCC 3 cut(s) 41, 204, 286
ApeKI GCWGC 2 cut(s) 95, 575
AspLEI GCGC 2 cut(s) 54, 95
AspS9I GGNCC 5 cut(s) 199, 245, 255, 287, 501
AsuC2I CCSGG 1 cut(s) 290
AsuHPI GGTGA 1 cut(s) 74
AvaI CYCGRG 2 cut(s) 218, 611
AvaII GGWCC 4 cut(s) 199, 245, 255, 501
Bbv12I GWGCWC 1 cut(s) 219
BbvI GCAGC 2 cut(s) 82, 562
BccI CCATC 1 cut(s) 122
BceAI ACGGC 1 cut(s) 298
BcgI CGANNNNNNTGC 4 cut(s) 291, 325, 632, 666
BciT130I CCWGG 3 cut(s) 203, 521, 708
BcnI CCSGG 1 cut(s) 290
BcoDI GTCTC 2 cut(s) 287, 368
BfoI RGCGCY 1 cut(s) 96
BglI GCCNNNNNGGC 1 cut(s) 49
BisI GCNGC 5 cut(s) 55, 96, 112, 286, 576
BlsI GCNGC 5 cut(s) 56, 97, 113, 287, 577
Bme1390I CCNGG 4 cut(s) 203, 290, 521, 708
Bme18I GGWCC 4 cut(s) 199, 245, 255, 501
BmeT110I CYCGRG 2 cut(s) 218, 611
BmgT120I GGNCC 5 cut(s) 199, 245, 255, 287, 501
BmiI GGNNCC 5 cut(s) 152, 247, 256, 502, 503
BmrFI CCNGG 4 cut(s) 203, 290, 521, 708
BoxI GACNNNNGTC 1 cut(s) 260
BpmI CTGGAG 2 cut(s) 58, 585
BpuMI CCSGG 1 cut(s) 290
Bsa29I ATCGAT 1 cut(s) 652
BsaI GGTCTC 2 cut(s) 287, 368
BsaJI CCNNGG 1 cut(s) 520
Bsc4I CCNNNNNNNGG 3 cut(s) 403, 427, 511
Bse1I ACTGG 2 cut(s) 568, 583
BseBI CCWGG 3 cut(s) 203, 521, 708
BseCI ATCGAT 1 cut(s) 652
BseDI CCNNGG 1 cut(s) 520
BseGI GGATG 1 cut(s) 133
BseLI CCNNNNNNNGG 3 cut(s) 403, 427, 511
BseMII CTCAG 1 cut(s) 85
BseNI ACTGG 2 cut(s) 568, 583
BseRI GAGGAG 2 cut(s) 132, 629
BseXI GCAGC 2 cut(s) 82, 562
Bsh1236I CGCG 2 cut(s) 54, 173
BshFI GGCC 3 cut(s) 43, 206, 288
BshVI ATCGAT 1 cut(s) 652
BsiHKAI GWGCWC 1 cut(s) 219
BsiHKCI CYCGRG 2 cut(s) 218, 611
BsiSI CCGG 1 cut(s) 290
BslFI GGGAC 3 cut(s) 81, 268, 514
BslI CCNNNNNNNGG 3 cut(s) 403, 427, 511
BsmAI GTCTC 2 cut(s) 287, 368
BsmFI GGGAC 3 cut(s) 81, 268, 514
BsnI GGCC 3 cut(s) 43, 206, 288
Bso31I GGTCTC 2 cut(s) 287, 368
BsoBI CYCGRG 2 cut(s) 218, 611
Bsp1286I GDGCHC 1 cut(s) 219
Bsp143I GATC 4 cut(s) 223, 234, 579, 653
BspACI CCGC 8 cut(s) 54, 111, 114, 194, 285, 297, 347, 417
BspANI GGCC 3 cut(s) 43, 206, 288
BspCNI CTCAG 1 cut(s) 84
BspDI ATCGAT 1 cut(s) 652
BspFNI CGCG 2 cut(s) 54, 173
BspLI GGNNCC 5 cut(s) 152, 247, 256, 502, 503
BspPI GGATC 3 cut(s) 231, 574, 648
BspTNI GGTCTC 2 cut(s) 287, 368
BsrBI CCGCTC 1 cut(s) 299
BsrI ACTGG 2 cut(s) 568, 583
BssECI CCNNGG 1 cut(s) 520
BssMI GATC 4 cut(s) 223, 234, 579, 653
BssNAI GTATAC 1 cut(s) 271
Bst1107I GTATAC 1 cut(s) 271
Bst2UI CCWGG 3 cut(s) 203, 521, 708
Bst4CI ACNGT 2 cut(s) 62, 463
BstDEI CTNAG 1 cut(s) 71
BstF5I GGATG 1 cut(s) 133
BstFNI CGCG 2 cut(s) 54, 173
BstH2I RGCGCY 1 cut(s) 96
BstHHI GCGC 2 cut(s) 54, 95
BstKTI GATC 4 cut(s) 226, 237, 582, 656
BstMAI GTCTC 2 cut(s) 287, 368
BstMBI GATC 4 cut(s) 223, 234, 579, 653
BstMWI GCNNNNNNNGC 2 cut(s) 49, 344
BstNI CCWGG 3 cut(s) 203, 521, 708
BstPAI GACNNNNGTC 1 cut(s) 260
BstSCI CCNGG 4 cut(s) 201, 288, 519, 706
BstUI CGCG 2 cut(s) 54, 173
BstV1I GCAGC 2 cut(s) 82, 562
BstZ17I GTATAC 1 cut(s) 271
Bsu15I ATCGAT 1 cut(s) 652
BsuRI GGCC 3 cut(s) 43, 206, 288
BsuTUI ATCGAT 1 cut(s) 652
BtsCI GGATG 1 cut(s) 133
BtsIMutI CAGTG 2 cut(s) 561, 591
CfoI GCGC 2 cut(s) 54, 95
Cfr13I GGNCC 5 cut(s) 199, 245, 255, 287, 501
ClaI ATCGAT 1 cut(s) 652
Csp6I GTAC 1 cut(s) 169
CviAII CATG 3 cut(s) 471, 626, 678
CviJI RGCY 7 cut(s) 43, 75, 206, 231, 288, 433, 631
CviKI_1 RGCY 7 cut(s) 43, 75, 206, 231, 288, 433, 631
CviQI GTAC 1 cut(s) 169
DdeI CTNAG 1 cut(s) 71
DpnI GATC 4 cut(s) 225, 236, 581, 655
DpnII GATC 4 cut(s) 223, 234, 579, 653
DraI TTTAAA 1 cut(s) 673
EciI GGCGGA 1 cut(s) 129
Eco31I GGTCTC 2 cut(s) 287, 368
Eco47I GGWCC 4 cut(s) 199, 245, 255, 501
Eco57I CTGAAG 1 cut(s) 375
Eco88I CYCGRG 2 cut(s) 218, 611
EcoO109I RGGNCCY 2 cut(s) 255, 501
EcoRII CCWGG 3 cut(s) 201, 519, 706
FaeI CATG 3 cut(s) 474, 629, 681
FaiI YATR 7 cut(s) 191, 271, 472, 491, 493, 627, 679
FaqI GGGAC 3 cut(s) 81, 268, 514
FatI CATG 3 cut(s) 470, 625, 677
FblI GTMKAC 1 cut(s) 270
Fnu4HI GCNGC 5 cut(s) 55, 96, 112, 286, 576
FokI GGATG 1 cut(s) 140
Fsp4HI GCNGC 5 cut(s) 55, 96, 112, 286, 576
GlaI GCGC 2 cut(s) 53, 94
GluI GCNGC 5 cut(s) 55, 96, 112, 286, 576
GsuI CTGGAG 2 cut(s) 58, 585
HaeII RGCGCY 1 cut(s) 96
HaeIII GGCC 3 cut(s) 43, 206, 288
HapII CCGG 1 cut(s) 290
HhaI GCGC 2 cut(s) 54, 95
Hin1II CATG 3 cut(s) 474, 629, 681
Hin6I GCGC 2 cut(s) 52, 93
HinP1I GCGC 2 cut(s) 52, 93
HinfI GANTC 6 cut(s) 19, 69, 84, 239, 350, 527
HpaII CCGG 1 cut(s) 290
HphI GGTGA 1 cut(s) 74
Hpy166II GTNNAC 2 cut(s) 271, 414
Hpy188I TCNGA 6 cut(s) 155, 228, 326, 355, 398, 430
Hpy188III TCNNGA 2 cut(s) 243, 611
Hpy8I GTNNAC 2 cut(s) 271, 414
Hpy99I CGWCG 4 cut(s) 62, 92, 266, 278
HpyAV CCTTC 3 cut(s) 268, 350, 401
HpyCH4III ACNGT 2 cut(s) 62, 463
HpyCH4IV ACGT 1 cut(s) 306
HpyCH4V TGCA 2 cut(s) 605, 643
HpyF10VI GCNNNNNNNGC 2 cut(s) 49, 344
HpyF3I CTNAG 1 cut(s) 71
HpySE526I ACGT 1 cut(s) 306
Hsp92II CATG 3 cut(s) 474, 629, 681
HspAI GCGC 2 cut(s) 52, 93
KflI GGGWCCC 1 cut(s) 501
Kzo9I GATC 4 cut(s) 223, 234, 579, 653
LmnI GCTCC 1 cut(s) 430
Lsp1109I GCAGC 2 cut(s) 82, 562
MaeII ACGT 1 cut(s) 306
MaeIII GTNAC 1 cut(s) 364
MalI GATC 4 cut(s) 225, 236, 581, 655
MbiI CCGCTC 1 cut(s) 299
MboI GATC 4 cut(s) 223, 234, 579, 653
MhlI GDGCHC 1 cut(s) 219
MluCI AATT 2 cut(s) 316, 661
MluI ACGCGT 1 cut(s) 171
MlyI GAGTC 3 cut(s) 63, 248, 359
MmeI TCCRAC 2 cut(s) 61, 463
MseI TTAA 1 cut(s) 672
MspA1I CMGCKG 1 cut(s) 347
MspI CCGG 1 cut(s) 290
MspR9I CCNGG 4 cut(s) 203, 290, 521, 708
MvaI CCWGG 3 cut(s) 203, 521, 708
MvnI CGCG 2 cut(s) 54, 173
MwoI GCNNNNNNNGC 2 cut(s) 49, 344
NciI CCSGG 1 cut(s) 290
NdeII GATC 4 cut(s) 223, 234, 579, 653
NlaIII CATG 3 cut(s) 474, 629, 681
NlaIV GGNNCC 5 cut(s) 152, 247, 256, 502, 503
NmuCI GTSAC 1 cut(s) 364
PaeR7I CTCGAG 2 cut(s) 218, 611
PcsI WCGNNNNNNNCGW 2 cut(s) 177, 270
PfeI GAWTC 3 cut(s) 19, 84, 527
PkrI GCNGC 5 cut(s) 56, 97, 113, 287, 577
PleI GAGTC 3 cut(s) 63, 247, 358
PpsI GAGTC 3 cut(s) 63, 247, 358
PpuMI RGGWCCY 2 cut(s) 255, 501
PshAI GACNNNNGTC 1 cut(s) 260
Psp5II RGGWCCY 2 cut(s) 255, 501
Psp6I CCWGG 3 cut(s) 201, 519, 706
PspGI CCWGG 3 cut(s) 201, 519, 706
PspN4I GGNNCC 5 cut(s) 152, 247, 256, 502, 503
PspPI GGNCC 5 cut(s) 199, 245, 255, 287, 501
PspPPI RGGWCCY 2 cut(s) 255, 501
PspXI VCTCGAGB 1 cut(s) 218
RsaI GTAC 1 cut(s) 170
RsaNI GTAC 1 cut(s) 169
SaqAI TTAA 1 cut(s) 672
SatI GCNGC 5 cut(s) 55, 96, 112, 286, 576
Sau3AI GATC 4 cut(s) 223, 234, 579, 653
Sau96I GGNCC 5 cut(s) 199, 245, 255, 287, 501
SchI GAGTC 3 cut(s) 63, 248, 359
ScrFI CCNGG 4 cut(s) 203, 290, 521, 708
SduI GDGCHC 1 cut(s) 219
Sfr274I CTCGAG 2 cut(s) 218, 611
SinI GGWCC 4 cut(s) 199, 245, 255, 501
SlaI CTCGAG 2 cut(s) 218, 611
SmlI CTYRAG 2 cut(s) 218, 611
SmoI CTYRAG 2 cut(s) 218, 611
Sse9I AATT 2 cut(s) 316, 661
SsiI CCGC 8 cut(s) 54, 111, 114, 194, 285, 297, 347, 417
StyD4I CCNGG 4 cut(s) 201, 288, 519, 706
TaaI ACNGT 2 cut(s) 62, 463
TaiI ACGT 1 cut(s) 309
TaqI TCGA 5 cut(s) 219, 237, 301, 612, 652
TasI AATT 2 cut(s) 316, 661
TauI GCSGC 3 cut(s) 57, 114, 288
TfiI GAWTC 3 cut(s) 19, 84, 527
Tru1I TTAA 1 cut(s) 672
Tru9I TTAA 1 cut(s) 672
TscAI CASTG 2 cut(s) 568, 598
TseFI GTSAC 1 cut(s) 364
TseI GCWGC 2 cut(s) 95, 575
Tsp45I GTSAC 1 cut(s) 364
TspDTI ATGAA 1 cut(s) 32
TspGWI ACGGA 2 cut(s) 120, 291
TspRI CASTG 2 cut(s) 568, 598
VpaK11BI GGWCC 4 cut(s) 199, 245, 255, 501
XcmI CCANNNNNNNNNTGG 1 cut(s) 239
XhoI CTCGAG 2 cut(s) 218, 611
XmiI GTMKAC 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.