pycom16g14470
MYB Family

Protein ALWAYS EARLY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0003854
Physical Location & Seq
Reverse (-)
10533623 .. 10542045
8423 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3240 bp
ATGGTAGAGGCCCTTTACACAATGAATAAGGCCTATTTATCTCTTCCTGAAGGTGTTGCTTCTGTTATTGGTCTGACAGCAATGATGACTGACCACTACTCTCTACTGGGAGGAAGTGACAGTGAACAAGAAAGCATTGAGGGTGTAGGAACTCCTTGGAAACCTCAAAAGCATGAAAGGGTAAAATTTCAGACTGAGGTCTCAAAAAGATTTGGACATATTCCAGATTCTTCACAATCCCCTCCAATGGCATCAAGTAACGACTGCTTGTCACTGTTGAACAATAGGCGCTCAGGAATTAGGCCTCATGCTGTTAAAAAAAGGACTCCTCGAGTCCCTGTTGCATATTCTAGTGATAAAGATAACAGTAGAAATTATGTTTCACCAGCCAGGCAAGGTTTAAAACCAATTGTAGACACCAAAAATGATGATGTTGCTAATGAGATAGCATTGGCATTGACTGAGGCCTCACATAGAGGTGATTCTCCATTGGTTTCTTGGAAATCTAAGAGAAAAGCCAAAGGTACACCACCAACTGTTAGGAATGGTGAAATGATGGGTGCAGAATCGGAAATGACTACAACCAGGCTCCGTGCTGGTGAAATGGATGAGGGTGGACATGAATTGAGCTTAGGAAGCACCGAAGCTGACAATGAAGACTTTGACAGAAGTAAAAAGTATGCAGTGGGTAAAGAAGGTACTGATACTCTGGACTTCCAACGGAGGGGGAAGAGATACTCTGTAAAGAAGCAAAGGGATGAAAATAAAAGCAATCATTTTGAAGACATTAAAGAAGCCGGTAGCGGAACAGAGGAAGGACAGAAGCGAGGTACTATCAAGGGAAAACTTGAAACTAAAGTTGCGAAAAGTGCAAGATCCTTTTACAAGGAACAAAGAAACAAAAGTAAAAAAGCTCTGATTGCAGAAGATGAAAACACTCCCTTTGATGCACTACAAACTCTGGCAGATCTATCCTTGATGATGCCCGAAACTGCTGATACAGAGTCTTCTGCACATGTCAGGGAAGAGGATAGTTATGTTGCTAACAAGTCTAAAACAAAAGGGATTCACTCAATTCCTGGGGTTGAACACAGTGCTTTGAAAACATCTAAACCGGGCAAACTGAAAGAGGGAGGTCACCAGTCTAACACCGGACTGCAAAAAAGAAAGCAATTGTTGCCATTTAAAGTATACAATGACGCAATTCAAACAGACTGTCGTTGGAGTGACAATCAAAAGATTGAGGCTACAGTTGAGGTGAATAAGTCTATGAATAAAGGTAAACGGTCTTCTCATTATATTACACACCCAAAGCAAGGGAAATCAATGAATCCTCTACGGAATGCATCTTCTTCTACTGATCATGAAGGGGAGGAGAATAATTCTGGATTATCAGCTTTAGGAGTACCATCTGCAAACCAGGCCAACTTACCTGCAAGAGATAGTATTAAATTGAAGCTGGACAGGCACAAACCATCAATAGAGAAGGATAGAAAGTCTACTGAGGGTATTTTAGATGAACAGCCTGATAAACTGGTTCCTTCATTTCGTAACAGCGAAATAAATATTAAGGAAAGACTTTCTAATTGCTTGTCTCGGTATCAAGTGCGAAGATGGTGTGCTTTTGAATGGTTTTATAGTGCAATTGATTACCCATGGTTTGCTAAAAGGGAGTTTGTGGAGTACTTGAATCATGTTGGATTAGGTCATGTTCCAAGATTAACTCGTGTTGAATGGGGTGTAATACGGAGTTCCCTTGGCAGACCGAGGAGATTTTCTGAGCAGTTTCTGAAGGAAGAAAAGGAGAAGCTTAATCAGTACCGAGAATCTGTTAGAACACATTATGCCGAACTTAATGCTGGAACAAGGGAAGGGCTTCCAACTGACTTAGCTCGGCCTTTATCAGTAGGACAGCATGTCATTGCTTTTCATCCCAGGACTAGAGAGATTCTCAATGGAATTGTACTAACCGTTGACCATAGTAGATGTCGTGTTCAGTTTGATCAGCCTGAACTAGGGGTTGAACATATTATGGATATTGATTGCATGCCTTTAAATCCAGTGGAGAATTTACCTGCTTCTTTCACAAAGCACAATGTCAATGTCAATGTCAACAAATATATTGAGAACCTAAAGGAGCTCAAGGGTAATGAACGCCTTAAAGAAGGAAAGACTGAAGGGTATAAGGTTGCCTCTAGTGACCATGTGGAGAGCACTGTTCCCTGTTATATGCAGCCATCGCCTCATCAGATCAACAAATCATCAAAGCAGACAGAGGGGAAATCATCGAATTTTGTACAAGCTAAAGGTGGGCCCCATGAAACTGCTGCTGCACAAGTAGCAAATTCTCAGCTTTCACTTCCTGTGCAGATCCAAGCAAAAGAGGCTGATATACGAGCTATATTTGAGTTGACTCGTGCACTTGACAAAAAGGATGCTGTGGTATCTGAGTTGAGGCACATGAATGATGAGGTGTTGGAAAACCAAAGAGATGGAGACAACTTTATCAGGGATTCTGAGCCTTTTAAAAAGGAATATGCTGCGGTGCTCTTACAGTTGAGTCAAGTCAATGATCAGGTTTCTTCTGCTCTTTTTTGCTTAAGACAACGCAACACATATAGAGGGAGTCCCGCAGTTAGCTTGGTGAAGCCCATGGCAAATTTAAGCGACCCTGGTGGCCATCCAAGCCCTTCTGGGTATCCTTGTCATGCCCAAGAATCGGCAGGTCATGTGTTTGATATTGTTGAAAGTTCAAGAGCAAAAGCCCGCAAAATGGTTGATGCAGCTATGCAGGCAATTTCATCATTGAGGAAGGAAGATAAACTTGATAGGATGGAGGAGATTATAGACTTTGTTAGTAATCGGCTCTCTGATGACGCTGGCATGCTGGGACCTTCTACTACCCCTGCAGATCCAGTTCGGGTTTCTGAGGATCAGCCAACTGCCTGTACATCAAAACCAATGGAAACGAGTTGTTCAGCTGATCCCAAGTCAAATAGCGTATCGAAGCGAAATGAAGAAAAAGCCCTTACAGACCTTATTATAAATTGTGTTGCAGCGTTTTTGATGATTCAGACATGTACAGCGCGACAGTTTCCACCGTCTGAAGTCGCCCAGGTATTAGACGAAGCCGTGACTAGTTTGCAGCCACTTTGTCCGCAGAACCTTTCGGTTTACGGAGAAATACAGAAGTGCATGGGCATTATTAGGAACCAGATACTGGCGTTGGTGCCTACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04218 map04218
Pfam Domains
Protein Families
DIRP Myb_DNA-binding

Protein Analysis

1080

Amino Acids

120.16

Weight (kDa)

8.44

Isoelectric Point (pI)

49.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIRP PF06584 544 - 645 2.3e-33 DIRP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3044
Acc36I ACCTGC 3 cut(s) 1441, 2083, 2714
AccB1I GGYRCC 1 cut(s) 3229
AccB7I CCANNNNNTGG 1 cut(s) 3220
AccI GTMKAC 3 cut(s) 414, 1191, 1499
AccII CGCG 1 cut(s) 3088
AciI CCGC 5 cut(s) 804, 2543, 2631, 2767, 3158
AclWI GGATC 5 cut(s) 870, 2365, 2906, 2940, 2978
AcoI YGGCCR 1 cut(s) 2677
AcsI RAATTY 5 cut(s) 185, 2068, 2290, 2344, 2659
AcuI CTGAAG 4 cut(s) 69, 1811, 2196, 3126
AfiI CCNNNNNNNGG 7 cut(s) 247, 724, 1316, 2015, 2719, 2773, 3220
AflII CTTAAG 1 cut(s) 2599
AflIII ACRYGT 2 cut(s) 1015, 3077
AhdI GACNNNNNGTC 2 cut(s) 268, 1916
AhlI ACTAGT 1 cut(s) 3137
AjnI CCWGG 7 cut(s) 389, 584, 1078, 1419, 1934, 2671, 3114
Alw21I GWGCWC 4 cut(s) 2142, 2216, 2422, 2550
Alw26I GTCTC 3 cut(s) 205, 1599, 2490
Alw44I GTGCAC 1 cut(s) 2418
AlwI GGATC 5 cut(s) 870, 2365, 2906, 2940, 2978
AlwNI CAGNNNCTG 2 cut(s) 1789, 3220
Ama87I CYCGRG 1 cut(s) 330
AoxI GGCC 8 cut(s) 9, 30, 302, 465, 1422, 1895, 2312, 2677
ApaI GGGCCC 1 cut(s) 2316
ApaLI GTGCAC 1 cut(s) 2418
ApeKI GCWGC 7 cut(s) 2233, 2327, 2330, 2540, 2783, 3056, 3145
ApoI RAATTY 5 cut(s) 185, 2068, 2290, 2344, 2659
ArsI GACNNNNNNTTYG 2 cut(s) 1201, 1233
Asp700I GAANNNNTTC 1 cut(s) 1875
AspLEI GCGC 2 cut(s) 291, 3088
AspS9I GGNCC 4 cut(s) 10, 2312, 2313, 2891
AsuC2I CCSGG 1 cut(s) 1116
AsuHPI GGTGA 7 cut(s) 375, 491, 560, 611, 1130, 1270, 2656
AvaI CYCGRG 1 cut(s) 330
AvaII GGWCC 1 cut(s) 2891
BaeGI GKGCMC 2 cut(s) 2316, 2422
BaeI ACNNNNGTAYC 1 cut(s) 3209
BalI TGGCCA 1 cut(s) 2679
BanI GGYRCC 1 cut(s) 3229
BanII GRGCYC 2 cut(s) 2142, 2316
BarI GAAGNNNNNNTAC 2 cut(s) 1273, 1305
BauI CACGAG 2 cut(s) 1725, 2415
BbsI GAAGAC 4 cut(s) 663, 789, 999, 1281
Bbv12I GWGCWC 4 cut(s) 2142, 2216, 2422, 2550
BbvI GCAGC 7 cut(s) 2245, 2314, 2317, 2527, 2795, 3068, 3157
BccI CCATC 8 cut(s) 550, 1417, 1483, 1608, 2245, 2486, 2688, 2827
BceAI ACGGC 1 cut(s) 3116
BcgI CGANNNNNNTGC 2 cut(s) 1838, 1872
BciT130I CCWGG 7 cut(s) 391, 586, 1080, 1421, 1936, 2673, 3116
BciVI GTATCC 1 cut(s) 2709
BclI TGATCA 3 cut(s) 1360, 2002, 2572
BcnI CCSGG 1 cut(s) 1116
BcoDI GTCTC 3 cut(s) 205, 1599, 2490
BcuI ACTAGT 1 cut(s) 3137
BfaI CTAG 5 cut(s) 351, 1941, 2015, 2196, 3138
BfmI CTRYAG 2 cut(s) 1248, 2907
BfoI RGCGCY 1 cut(s) 292
BfrI CTTAAG 1 cut(s) 2599
BfuAI ACCTGC 3 cut(s) 1441, 2083, 2714
BfuI GTATCC 1 cut(s) 2709
BglII AGATCT 1 cut(s) 967
BisI GCNGC 7 cut(s) 2234, 2328, 2331, 2541, 2784, 3057, 3146
BlsI GCNGC 7 cut(s) 2235, 2329, 2332, 2542, 2785, 3058, 3147
BmcAI AGTACT 1 cut(s) 1685
Bme1390I CCNGG 8 cut(s) 391, 586, 1080, 1116, 1421, 1936, 2673, 3116
Bme18I GGWCC 1 cut(s) 2891
BmeRI GACNNNNNGTC 2 cut(s) 268, 1916
BmeT110I CYCGRG 1 cut(s) 330
BmgT120I GGNCC 4 cut(s) 10, 2312, 2313, 2891
BmiI GGNNCC 7 cut(s) 590, 1539, 2314, 2315, 2892, 3212, 3231
BmrFI CCNGG 8 cut(s) 391, 586, 1080, 1116, 1421, 1936, 2673, 3116
BmrI ACTGGG 1 cut(s) 116
BmsI GCATC 6 cut(s) 260, 937, 972, 1355, 2425, 2770
BmuI ACTGGG 1 cut(s) 116
BoxI GACNNNNGTC 1 cut(s) 197
BpiI GAAGAC 4 cut(s) 663, 789, 999, 1281
BplI GAGNNNNNCTC 2 cut(s) 1935, 1967
Bpu10I CCTNAGC 2 cut(s) 292, 631
BpuEI CTTGAG 1 cut(s) 2126
BpuMI CCSGG 1 cut(s) 1116
BsaI GGTCTC 1 cut(s) 205
BsaJI CCNNGG 9 cut(s) 155, 1079, 1655, 1756, 1766, 1934, 2652, 2671, 3114
BsaWI WCCGGW 1 cut(s) 1151
Bsc4I CCNNNNNNNGG 7 cut(s) 247, 724, 1316, 2015, 2719, 2773, 3220
Bse118I RCCGGY 1 cut(s) 797
Bse1I ACTGG 6 cut(s) 111, 1141, 1539, 2060, 2915, 3225
Bse3DI GCAATG 2 cut(s) 87, 1920
BseBI CCWGG 7 cut(s) 391, 586, 1080, 1421, 1936, 2673, 3116
BseDI CCNNGG 9 cut(s) 155, 1079, 1655, 1756, 1766, 1934, 2652, 2671, 3114
BseGI GGATG 6 cut(s) 613, 763, 1930, 2440, 2680, 2838
BseLI CCNNNNNNNGG 7 cut(s) 247, 724, 1316, 2015, 2719, 2773, 3220
BseMI GCAATG 2 cut(s) 87, 1920
BseMII CTCAG 9 cut(s) 186, 306, 453, 1494, 1770, 2363, 2439, 2508, 2919
BseNI ACTGG 6 cut(s) 111, 1141, 1539, 2060, 2915, 3225
BseRI GAGGAG 4 cut(s) 318, 1388, 1783, 2852
BseSI GKGCMC 2 cut(s) 2316, 2422
BseXI GCAGC 7 cut(s) 2245, 2314, 2317, 2527, 2795, 3068, 3157
BseYI CCCAGC 1 cut(s) 2887
BsgI GTGCAG 4 cut(s) 582, 996, 2316, 2387
Bsh1236I CGCG 1 cut(s) 3088
BshFI GGCC 8 cut(s) 11, 32, 304, 467, 1424, 1897, 2314, 2679
BshNI GGYRCC 1 cut(s) 3229
BsiHKAI GWGCWC 4 cut(s) 2142, 2216, 2422, 2550
BsiHKCI CYCGRG 1 cut(s) 330
BsiSI CCGG 3 cut(s) 798, 1115, 1152
BslFI GGGAC 3 cut(s) 320, 2613, 2904
BslI CCNNNNNNNGG 7 cut(s) 247, 724, 1316, 2015, 2719, 2773, 3220
BsmAI GTCTC 3 cut(s) 205, 1599, 2490
BsmFI GGGAC 3 cut(s) 320, 2613, 2904
BsmI GAATGC 1 cut(s) 1348
BsnI GGCC 8 cut(s) 11, 32, 304, 467, 1424, 1897, 2314, 2679
Bso31I GGTCTC 1 cut(s) 205
BsoBI CYCGRG 1 cut(s) 330
Bsp120I GGGCCC 1 cut(s) 2312
Bsp1286I GDGCHC 5 cut(s) 2142, 2216, 2316, 2422, 2550
Bsp1407I TGTACA 3 cut(s) 2296, 2948, 3080
Bsp19I CCATGG 2 cut(s) 1655, 2652
BspACI CCGC 5 cut(s) 804, 2543, 2631, 2767, 3158
BspANI GGCC 8 cut(s) 11, 32, 304, 467, 1424, 1897, 2314, 2679
BspCNI CTCAG 9 cut(s) 187, 305, 454, 1495, 1771, 2362, 2440, 2509, 2920
BspFNI CGCG 1 cut(s) 3088
BspHI TCATGA 1 cut(s) 1363
BspLI GGNNCC 7 cut(s) 590, 1539, 2314, 2315, 2892, 3212, 3231
BspMAI CTGCAG 1 cut(s) 2911
BspMI ACCTGC 3 cut(s) 1441, 2083, 2714
BspPI GGATC 5 cut(s) 870, 2365, 2906, 2940, 2978
BspT107I GGYRCC 1 cut(s) 3229
BspTI CTTAAG 1 cut(s) 2599
BspTNI GGTCTC 1 cut(s) 205
BsrDI GCAATG 2 cut(s) 87, 1920
BsrFI RCCGGY 1 cut(s) 797
BsrGI TGTACA 3 cut(s) 2296, 2948, 3080
BsrI ACTGG 6 cut(s) 111, 1141, 1539, 2060, 2915, 3225
BssAI RCCGGY 1 cut(s) 797
BssECI CCNNGG 9 cut(s) 155, 1079, 1655, 1756, 1766, 1934, 2652, 2671, 3114
BssNAI GTATAC 1 cut(s) 1192
BssSI CACGAG 2 cut(s) 1725, 2415
BssT1I CCWWGG 4 cut(s) 155, 1655, 1756, 2652
Bst1107I GTATAC 1 cut(s) 1192
Bst2BI CACGAG 2 cut(s) 1725, 2415
Bst2UI CCWGG 7 cut(s) 391, 586, 1080, 1421, 1936, 2673, 3116
Bst6I CTCTTC 3 cut(s) 48, 725, 1020
BstAFI CTTAAG 1 cut(s) 2599
BstAPI GCANNNNNTGC 1 cut(s) 1177
BstAUI TGTACA 3 cut(s) 2296, 2948, 3080
BstC8I GCNNGC 5 cut(s) 2048, 2767, 2793, 2881, 2885
BstDSI CCRYGG 2 cut(s) 1655, 2652
BstEII GGTNACC 1 cut(s) 1136
BstENI CCTNNNNNAGG 1 cut(s) 2013
BstF5I GGATG 6 cut(s) 613, 763, 1930, 2440, 2680, 2838
BstFNI CGCG 1 cut(s) 3088
BstH2I RGCGCY 1 cut(s) 292
BstHHI GCGC 2 cut(s) 291, 3088
BstMAI GTCTC 3 cut(s) 205, 1599, 2490
BstNI CCWGG 7 cut(s) 391, 586, 1080, 1421, 1936, 2673, 3116
BstNSI RCATGY 5 cut(s) 1019, 1919, 2050, 2887, 3081
BstPAI GACNNNNGTC 1 cut(s) 197
BstPI GGTNACC 1 cut(s) 1136
BstSCI CCNGG 8 cut(s) 389, 584, 1078, 1114, 1419, 1934, 2671, 3114
BstSFI CTRYAG 2 cut(s) 1248, 2907
BstSLI GKGCMC 2 cut(s) 2316, 2422
BstUI CGCG 1 cut(s) 3088
BstV1I GCAGC 7 cut(s) 2245, 2314, 2317, 2527, 2795, 3068, 3157
BstV2I GAAGAC 4 cut(s) 663, 789, 999, 1281
BstX2I RGATCY 4 cut(s) 875, 967, 2370, 2911
BstXI CCANNNNNNTGG 1 cut(s) 2492
BstYI RGATCY 4 cut(s) 875, 967, 2370, 2911
BstZ17I GTATAC 1 cut(s) 1192
BsuI GTATCC 1 cut(s) 2709
BsuRI GGCC 8 cut(s) 11, 32, 304, 467, 1424, 1897, 2314, 2679
BtgI CCRYGG 2 cut(s) 1655, 2652
BtgZI GCGATG 1 cut(s) 2223
BtsCI GGATG 6 cut(s) 613, 763, 1930, 2440, 2680, 2838
BtsI GCAGTG 1 cut(s) 690
BtsIMutI CAGTG 6 cut(s) 127, 272, 690, 1099, 2067, 2214
BveI ACCTGC 3 cut(s) 1441, 2083, 2714
Cac8I GCNNGC 5 cut(s) 2048, 2767, 2793, 2881, 2885
CaiI CAGNNNCTG 2 cut(s) 1789, 3220
CciI TCATGA 1 cut(s) 1363
CfoI GCGC 2 cut(s) 291, 3088
Cfr10I RCCGGY 1 cut(s) 797
Cfr13I GGNCC 4 cut(s) 10, 2312, 2313, 2891
CseI GACGC 2 cut(s) 1208, 2885
DraI TTTAAA 4 cut(s) 402, 1186, 2055, 2527
DriI GACNNNNNGTC 2 cut(s) 268, 1916
EaeI YGGCCR 1 cut(s) 2677
Eam1104I CTCTTC 3 cut(s) 48, 725, 1020
Eam1105I GACNNNNNGTC 2 cut(s) 268, 1916
EarI CTCTTC 3 cut(s) 48, 725, 1020
Ecl136II GAGCTC 1 cut(s) 2140
Eco130I CCWWGG 4 cut(s) 155, 1655, 1756, 2652
Eco147I AGGCCT 3 cut(s) 32, 304, 467
Eco24I GRGCYC 2 cut(s) 2142, 2316
Eco31I GGTCTC 1 cut(s) 205
Eco47I GGWCC 1 cut(s) 2891
Eco53kI GAGCTC 1 cut(s) 2140
Eco57I CTGAAG 4 cut(s) 69, 1811, 2196, 3126
Eco88I CYCGRG 1 cut(s) 330
Eco91I GGTNACC 1 cut(s) 1136
EcoICRI GAGCTC 1 cut(s) 2140
EcoNI CCTNNNNNAGG 1 cut(s) 2013
EcoO109I RGGNCCY 3 cut(s) 10, 2313, 2891
EcoO65I GGTNACC 1 cut(s) 1136
EcoRII CCWGG 7 cut(s) 389, 584, 1078, 1419, 1934, 2671, 3114
EcoT14I CCWWGG 4 cut(s) 155, 1655, 1756, 2652
EcoT22I ATGCAT 1 cut(s) 1348
EcoT38I GRGCYC 2 cut(s) 2142, 2316
ErhI CCWWGG 4 cut(s) 155, 1655, 1756, 2652
FalI AAGNNNNNCTT 2 cut(s) 2808, 2840
FaqI GGGAC 3 cut(s) 320, 2613, 2904
FauI CCCGC 2 cut(s) 2638, 2774
FbaI TGATCA 3 cut(s) 1360, 2002, 2572
FblI GTMKAC 3 cut(s) 414, 1191, 1499
Fnu4HI GCNGC 7 cut(s) 2234, 2328, 2331, 2541, 2784, 3057, 3146
FokI GGATG 6 cut(s) 620, 770, 1917, 2447, 2667, 2845
FriOI GRGCYC 2 cut(s) 2142, 2316
Fsp4HI GCNGC 7 cut(s) 2234, 2328, 2331, 2541, 2784, 3057, 3146
FspBI CTAG 5 cut(s) 351, 1941, 2015, 2196, 3138
GlaI GCGC 2 cut(s) 290, 3087
GluI GCNGC 7 cut(s) 2234, 2328, 2331, 2541, 2784, 3057, 3146
GsaI CCCAGC 1 cut(s) 2891
HaeII RGCGCY 1 cut(s) 292
HaeIII GGCC 8 cut(s) 11, 32, 304, 467, 1424, 1897, 2314, 2679
HapII CCGG 3 cut(s) 798, 1115, 1152
HgaI GACGC 2 cut(s) 1208, 2885
HhaI GCGC 2 cut(s) 291, 3088
Hin6I GCGC 2 cut(s) 289, 3086
HinP1I GCGC 2 cut(s) 289, 3086
HincII GTYRAC 3 cut(s) 1975, 2113, 2412
HindII GTYRAC 3 cut(s) 1975, 2113, 2412
HindIII AAGCTT 1 cut(s) 1808
HpaII CCGG 3 cut(s) 798, 1115, 1152
HphI GGTGA 7 cut(s) 375, 491, 560, 611, 1130, 1270, 2656
Hpy188III TCNNGA 7 cut(s) 47, 224, 294, 710, 1364, 1386, 2754
HspAI GCGC 2 cut(s) 289, 3086
Ksp22I TGATCA 3 cut(s) 1360, 2002, 2572
LmnI GCTCC 2 cut(s) 594, 2137
Lsp1109I GCAGC 7 cut(s) 2245, 2314, 2317, 2527, 2795, 3068, 3157
LweI GCATC 6 cut(s) 260, 937, 972, 1355, 2425, 2770
MaeI CTAG 5 cut(s) 351, 1941, 2015, 2196, 3138
MaeIII GTNAC 8 cut(s) 116, 257, 270, 1136, 1226, 1550, 2198, 3133
MfeI CAATTG 3 cut(s) 408, 1172, 1644
MflI RGATCY 4 cut(s) 875, 967, 2370, 2911
MhlI GDGCHC 5 cut(s) 2142, 2216, 2316, 2422, 2550
MlsI TGGCCA 1 cut(s) 2679
MluNI TGGCCA 1 cut(s) 2679
MlyI GAGTC 6 cut(s) 319, 342, 1013, 2407, 2569, 2635
MmeI TCCRAC 5 cut(s) 742, 1202, 1678, 1904, 2457
Mox20I TGGCCA 1 cut(s) 2679
Mph1103I ATGCAT 1 cut(s) 1348
MroXI GAANNNNTTC 1 cut(s) 1875
MscI TGGCCA 1 cut(s) 2679
MslI CAYNNNNRTG 2 cut(s) 477, 2463
Msp20I TGGCCA 1 cut(s) 2679
MspA1I CMGCKG 1 cut(s) 2981
MspCI CTTAAG 1 cut(s) 2599
MspI CCGG 3 cut(s) 798, 1115, 1152
MspR9I CCNGG 8 cut(s) 391, 586, 1080, 1116, 1421, 1936, 2673, 3116
MunI CAATTG 3 cut(s) 408, 1172, 1644
Mva1269I GAATGC 1 cut(s) 1348
MvaI CCWGG 7 cut(s) 391, 586, 1080, 1421, 1936, 2673, 3116
MvnI CGCG 1 cut(s) 3088
NciI CCSGG 1 cut(s) 1116
NcoI CCATGG 2 cut(s) 1655, 2652
NlaIV GGNNCC 7 cut(s) 590, 1539, 2314, 2315, 2892, 3212, 3231
NmeAIII GCCGAG 1 cut(s) 1873
NmuCI GTSAC 6 cut(s) 116, 270, 1136, 1226, 2198, 3133
NsiI ATGCAT 1 cut(s) 1348
NspI RCATGY 5 cut(s) 1019, 1919, 2050, 2887, 3081
PaeI GCATGC 2 cut(s) 2050, 2887
PaeR7I CTCGAG 1 cut(s) 330
PagI TCATGA 1 cut(s) 1363
PceI AGGCCT 3 cut(s) 32, 304, 467
PciI ACATGT 2 cut(s) 1015, 3077
PctI GAATGC 1 cut(s) 1348
PdmI GAANNNNTTC 1 cut(s) 1875
PflMI CCANNNNNTGG 1 cut(s) 3220
PkrI GCNGC 7 cut(s) 2235, 2329, 2332, 2542, 2785, 3058, 3147
PleI GAGTC 6 cut(s) 319, 341, 1012, 2407, 2568, 2634
PpsI GAGTC 6 cut(s) 319, 341, 1012, 2407, 2568, 2634
PpuMI RGGWCCY 1 cut(s) 2891
PscI ACATGT 2 cut(s) 1015, 3077
PshAI GACNNNNGTC 1 cut(s) 197
PsiI TTATAA 1 cut(s) 3044
Psp124BI GAGCTC 1 cut(s) 2142
Psp5II RGGWCCY 1 cut(s) 2891
Psp6I CCWGG 7 cut(s) 389, 584, 1078, 1419, 1934, 2671, 3114
PspEI GGTNACC 1 cut(s) 1136
PspFI CCCAGC 1 cut(s) 2887
PspGI CCWGG 7 cut(s) 389, 584, 1078, 1419, 1934, 2671, 3114
PspN4I GGNNCC 7 cut(s) 590, 1539, 2314, 2315, 2892, 3212, 3231
PspOMI GGGCCC 1 cut(s) 2312
PspPI GGNCC 4 cut(s) 10, 2312, 2313, 2891
PspPPI RGGWCCY 1 cut(s) 2891
PspXI VCTCGAGB 1 cut(s) 330
PstI CTGCAG 1 cut(s) 2911
PstNI CAGNNNCTG 2 cut(s) 1789, 3220
PsuI RGATCY 4 cut(s) 875, 967, 2370, 2911
PvuII CAGCTG 1 cut(s) 2981
RseI CAYNNNNRTG 2 cut(s) 477, 2463
SacI GAGCTC 1 cut(s) 2142
SatI GCNGC 7 cut(s) 2234, 2328, 2331, 2541, 2784, 3057, 3146
Sau96I GGNCC 4 cut(s) 10, 2312, 2313, 2891
ScaI AGTACT 1 cut(s) 1685
SchI GAGTC 6 cut(s) 319, 342, 1013, 2407, 2569, 2635
ScrFI CCNGG 8 cut(s) 391, 586, 1080, 1116, 1421, 1936, 2673, 3116
SduI GDGCHC 5 cut(s) 2142, 2216, 2316, 2422, 2550
SfaNI GCATC 6 cut(s) 260, 937, 972, 1355, 2425, 2770
SfcI CTRYAG 2 cut(s) 1248, 2907
Sfr274I CTCGAG 1 cut(s) 330
SinI GGWCC 1 cut(s) 2891
SlaI CTCGAG 1 cut(s) 330
SmiMI CAYNNNNRTG 2 cut(s) 477, 2463
SmlI CTYRAG 3 cut(s) 330, 2141, 2599
SmoI CTYRAG 3 cut(s) 330, 2141, 2599
SpeI ACTAGT 1 cut(s) 3137
SphI GCATGC 2 cut(s) 2050, 2887
SseBI AGGCCT 3 cut(s) 32, 304, 467
SsiI CCGC 5 cut(s) 804, 2543, 2631, 2767, 3158
SspI AATATT 1 cut(s) 1567
SspMI CTAG 5 cut(s) 351, 1941, 2015, 2196, 3138
SstI GAGCTC 1 cut(s) 2142
StuI AGGCCT 3 cut(s) 32, 304, 467
StyD4I CCNGG 8 cut(s) 389, 584, 1078, 1114, 1419, 1934, 2671, 3114
StyI CCWWGG 4 cut(s) 155, 1655, 1756, 2652
TaqI TCGA 3 cut(s) 331, 2288, 3005
TaqII GACCGA 1 cut(s) 1780
TatI WGTACW 5 cut(s) 1683, 1963, 2296, 2948, 3080
TscAI CASTG 6 cut(s) 127, 279, 690, 1099, 2067, 2221
TseFI GTSAC 6 cut(s) 116, 270, 1136, 1226, 2198, 3133
TseI GCWGC 7 cut(s) 2233, 2327, 2330, 2540, 2783, 3056, 3145
Tsp45I GTSAC 6 cut(s) 116, 270, 1136, 1226, 2198, 3133
TspGWI ACGGA 5 cut(s) 581, 736, 1354, 1762, 3192
TspRI CASTG 6 cut(s) 127, 279, 690, 1099, 2067, 2221
Van91I CCANNNNNTGG 1 cut(s) 3220
Vha464I CTTAAG 1 cut(s) 2599
VneI GTGCAC 1 cut(s) 2418
VpaK11BI GGWCC 1 cut(s) 2891
XagI CCTNNNNNAGG 1 cut(s) 2013
XapI RAATTY 5 cut(s) 185, 2068, 2290, 2344, 2659
XceI RCATGY 5 cut(s) 1019, 1919, 2050, 2887, 3081
XcmI CCANNNNNNNNNTGG 1 cut(s) 495
XhoI CTCGAG 1 cut(s) 330
XmiI GTMKAC 3 cut(s) 414, 1191, 1499
XmnI GAANNNNTTC 1 cut(s) 1875
XspI CTAG 5 cut(s) 351, 1941, 2015, 2196, 3138
ZrmI AGTACT 1 cut(s) 1685
Zsp2I ATGCAT 1 cut(s) 1348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.