Protein ALWAYS EARLY
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT3G21430 AT3G21430 AT3G21430 |
| fragaria_vesca | FvH4_4g17660 FvH4_4g17660 FvH4_4g17660 FvH4_4g17660 |
| malus_domestica | MD16G1172500.v1.1 MD16G1172600.v1.1 |
| prunus_persica | Prupe.1G139300_v2.0.a1 Prupe.1G139300_v2.0.a1 Prupe.1G139300_v2.0.a1 Prupe.1G139300_v2.0.a1 Prupe.1G139300_v2.0.a1 Prupe.1G139300_v2.0.a1 Prupe.1G139300_v2.0.a1 Prupe.1G139300_v2.0.a1 Prupe.1G139300_v2.0.a1 |
| pyrus_communis | pycom16g14470 |
| rosa_chinensis | RchiOBHm_Chr4g0421601 |
| rosa_laevigata | RLG00000007665 RLG00000008391 |
| rosa_multiflora | Rmu_ssc0000097.1_g000002 |
| rosa_roxburghii | Rroxscaffold_5G00363850 |
| rosa_rugosa | Rorug04G0173300 Rorug04G0173400 Rorug04G0173500 Rorug04G0173600 Rorug04G0173700 |
| rosa_samantha | Rh4AG233400 Rh4BG236300 Rh4CG248000 Rh4DG232200 |
| rosa_wichuraiana | Rw4G020070 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1913 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 742 |
| Acc36I | ACCTGC | 1 cut(s) | 2251 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 3323 |
| AccI | GTMKAC | 3 cut(s) | 1328, 1436, 1636 |
| AccII | CGCG | 1 cut(s) | 1777 |
| AciI | CCGC | 2 cut(s) | 1777, 2219 |
| AclWI | GGATC | 3 cut(s) | 2110, 3070, 3125 |
| AcoI | YGGCCR | 2 cut(s) | 621, 2848 |
| AcsI | RAATTY | 5 cut(s) | 1338, 1528, 2515, 2683, 2994 |
| AcuI | CTGAAG | 5 cut(s) | 168, 1185, 1236, 2358, 3117 |
| AcyI | GRCGYC | 2 cut(s) | 435, 737 |
| AflIII | ACRYGT | 3 cut(s) | 123, 2083, 3224 |
| AgeI | ACCGGT | 1 cut(s) | 225 |
| AhlI | ACTAGT | 1 cut(s) | 3284 |
| AjnI | CCWGG | 5 cut(s) | 623, 731, 1605, 2102, 3260 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 2463, 2495, 3076, 3108 |
| Alw21I | GWGCWC | 2 cut(s) | 2572, 2593 |
| Alw26I | GTCTC | 5 cut(s) | 186, 1620, 1671, 2486, 2767 |
| Alw44I | GTGCAC | 1 cut(s) | 2589 |
| AlwI | GGATC | 3 cut(s) | 2110, 3070, 3125 |
| Ama87I | CYCGRG | 1 cut(s) | 284 |
| ApaLI | GTGCAC | 1 cut(s) | 2589 |
| ApeKI | GCWGC | 5 cut(s) | 501, 873, 2711, 3077, 3203 |
| ApoI | RAATTY | 5 cut(s) | 1338, 1528, 2515, 2683, 2994 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 1103, 1135, 2810, 2842 |
| AseI | ATTAAT | 1 cut(s) | 2310 |
| AsiGI | ACCGGT | 1 cut(s) | 225 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 1747, 2937 |
| AspLEI | GCGC | 4 cut(s) | 528, 1129, 1777, 2631 |
| AspS9I | GGNCC | 6 cut(s) | 4, 131, 247, 267, 825, 2483 |
| AsuC2I | CCSGG | 2 cut(s) | 8, 1991 |
| AsuHPI | GGTGA | 5 cut(s) | 215, 609, 1459, 1525, 3237 |
| AvaI | CYCGRG | 1 cut(s) | 284 |
| BaeGI | GKGCMC | 2 cut(s) | 293, 2593 |
| BaeI | ACNNNNGTAYC | 4 cut(s) | 1017, 1050, 3323, 3356 |
| BalI | TGGCCA | 1 cut(s) | 2850 |
| BanII | GRGCYC | 1 cut(s) | 2572 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 2547, 2579 |
| BauI | CACGAG | 3 cut(s) | 567, 1893, 3374 |
| BbsI | GAAGAC | 7 cut(s) | 1116, 1278, 1467, 1750, 2339, 2445, 3171 |
| Bbv12I | GWGCWC | 2 cut(s) | 2572, 2593 |
| BbvI | GCAGC | 5 cut(s) | 488, 885, 2698, 3089, 3215 |
| BccI | CCATC | 7 cut(s) | 491, 644, 775, 839, 1776, 2033, 2657 |
| BceAI | ACGGC | 1 cut(s) | 2594 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 275, 309, 3331, 3365 |
| BciT130I | CCWGG | 5 cut(s) | 625, 733, 1607, 2104, 3262 |
| BclI | TGATCA | 1 cut(s) | 2743 |
| BcnI | CCSGG | 2 cut(s) | 8, 1991 |
| BcoDI | GTCTC | 5 cut(s) | 186, 1620, 1671, 2486, 2767 |
| BcuI | ACTAGT | 1 cut(s) | 3284 |
| BfaI | CTAG | 7 cut(s) | 66, 1029, 2109, 2183, 2499, 3285, 3368 |
| BfmI | CTRYAG | 5 cut(s) | 1437, 1542, 1670, 2712, 3075 |
| BfuAI | ACCTGC | 1 cut(s) | 2251 |
| BglII | AGATCT | 1 cut(s) | 1144 |
| BisI | GCNGC | 6 cut(s) | 502, 874, 2219, 2712, 3078, 3204 |
| BlsI | GCNGC | 6 cut(s) | 503, 875, 2220, 2713, 3079, 3205 |
| BmcAI | AGTACT | 4 cut(s) | 611, 763, 1853, 2718 |
| Bme1390I | CCNGG | 7 cut(s) | 8, 625, 733, 1607, 1991, 2104, 3262 |
| BmeT110I | CYCGRG | 1 cut(s) | 284 |
| BmgT120I | GGNCC | 6 cut(s) | 4, 131, 247, 267, 825, 2483 |
| BmiI | GGNNCC | 3 cut(s) | 6, 388, 827 |
| BmrFI | CCNGG | 7 cut(s) | 8, 625, 733, 1607, 1991, 2104, 3262 |
| BmrI | ACTGGG | 2 cut(s) | 569, 1031 |
| BmsI | GCATC | 8 cut(s) | 370, 500, 1114, 1149, 1648, 2532, 2596, 3031 |
| BmuI | ACTGGG | 2 cut(s) | 569, 1031 |
| BpiI | GAAGAC | 7 cut(s) | 1116, 1278, 1467, 1750, 2339, 2445, 3171 |
| BpmI | CTGGAG | 2 cut(s) | 376, 2827 |
| Bpu10I | CCTNAGC | 3 cut(s) | 672, 868, 2550 |
| BpuEI | CTTGAG | 4 cut(s) | 1174, 1642, 1721, 2791 |
| BpuMI | CCSGG | 2 cut(s) | 8, 1991 |
| Bsa29I | ATCGAT | 1 cut(s) | 1815 |
| BsaHI | GRCGYC | 2 cut(s) | 435, 737 |
| BsaWI | WCCGGW | 1 cut(s) | 225 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 668, 698, 754, 784, 1771, 1801 |
| Bse118I | RCCGGY | 4 cut(s) | 225, 619, 2027, 2881 |
| Bse3DI | GCAATG | 2 cut(s) | 324, 1218 |
| BseBI | CCWGG | 5 cut(s) | 625, 733, 1607, 2104, 3262 |
| BseCI | ATCGAT | 1 cut(s) | 1815 |
| BseGI | GGATG | 8 cut(s) | 798, 850, 1164, 2044, 2098, 2611, 2963, 3043 |
| BseMI | GCAATG | 2 cut(s) | 324, 1218 |
| BseRI | GAGGAG | 2 cut(s) | 555, 2403 |
| BseSI | GKGCMC | 2 cut(s) | 293, 2593 |
| BseXI | GCAGC | 5 cut(s) | 488, 885, 2698, 3089, 3215 |
| BsgI | GTGCAG | 2 cut(s) | 1176, 2558 |
| Bsh1236I | CGCG | 1 cut(s) | 1777 |
| BshTI | ACCGGT | 1 cut(s) | 225 |
| BshVI | ATCGAT | 1 cut(s) | 1815 |
| BsiHKAI | GWGCWC | 2 cut(s) | 2572, 2593 |
| BsiHKCI | CYCGRG | 1 cut(s) | 284 |
| BsiSI | CCGG | 7 cut(s) | 8, 226, 620, 1990, 2028, 2829, 2882 |
| BslFI | GGGAC | 1 cut(s) | 557 |
| BsmAI | GTCTC | 5 cut(s) | 186, 1620, 1671, 2486, 2767 |
| BsmFI | GGGAC | 1 cut(s) | 557 |
| BsoBI | CYCGRG | 1 cut(s) | 284 |
| Bsp1286I | GDGCHC | 3 cut(s) | 293, 2572, 2593 |
| Bsp1407I | TGTACA | 3 cut(s) | 1585, 3116, 3227 |
| Bsp143I | GATC | 9 cut(s) | 1144, 1546, 2115, 2301, 2743, 3062, 3100, 3130, 3271 |
| Bsp19I | CCATGG | 1 cut(s) | 1823 |
| BspACI | CCGC | 2 cut(s) | 1777, 2219 |
| BspDI | ATCGAT | 1 cut(s) | 1815 |
| BspFNI | CGCG | 1 cut(s) | 1777 |
| BspHI | TCATGA | 1 cut(s) | 3213 |
| BspLI | GGNNCC | 3 cut(s) | 6, 388, 827 |
| BspMAI | CTGCAG | 3 cut(s) | 1674, 2716, 3079 |
| BspMI | ACCTGC | 1 cut(s) | 2251 |
| BspPI | GGATC | 3 cut(s) | 2110, 3070, 3125 |
| BspQI | GCTCTTC | 1 cut(s) | 144 |
| BsrDI | GCAATG | 2 cut(s) | 324, 1218 |
| BsrFI | RCCGGY | 4 cut(s) | 225, 619, 2027, 2881 |
| BsrGI | TGTACA | 3 cut(s) | 1585, 3116, 3227 |
| BssAI | RCCGGY | 4 cut(s) | 225, 619, 2027, 2881 |
| BssMI | GATC | 9 cut(s) | 1144, 1546, 2115, 2301, 2743, 3062, 3100, 3130, 3271 |
| BssNI | GRCGYC | 2 cut(s) | 435, 737 |
| BssSI | CACGAG | 3 cut(s) | 567, 1893, 3374 |
| BssT1I | CCWWGG | 3 cut(s) | 1409, 1823, 1924 |
| Bst2BI | CACGAG | 3 cut(s) | 567, 1893, 3374 |
| Bst2UI | CCWGG | 5 cut(s) | 625, 733, 1607, 2104, 3262 |
| Bst4CI | ACNGT | 8 cut(s) | 359, 513, 1326, 1473, 1585, 2727, 3146, 3240 |
| Bst6I | CTCTTC | 5 cut(s) | 144, 285, 1096, 1120, 2966 |
| BstACI | GRCGYC | 2 cut(s) | 435, 737 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1049 |
| BstAUI | TGTACA | 3 cut(s) | 1585, 3116, 3227 |
| BstDSI | CCRYGG | 4 cut(s) | 829, 1823, 1934, 2610 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 2971 |
| BstF5I | GGATG | 8 cut(s) | 798, 850, 1164, 2044, 2098, 2611, 2963, 3043 |
| BstFNI | CGCG | 1 cut(s) | 1777 |
| BstHHI | GCGC | 4 cut(s) | 528, 1129, 1777, 2631 |
| BstKTI | GATC | 9 cut(s) | 1147, 1549, 2118, 2304, 2746, 3065, 3103, 3133, 3274 |
| BstMAI | GTCTC | 5 cut(s) | 186, 1620, 1671, 2486, 2767 |
| BstMBI | GATC | 9 cut(s) | 1144, 1546, 2115, 2301, 2743, 3062, 3100, 3130, 3271 |
| BstNI | CCWGG | 5 cut(s) | 625, 733, 1607, 2104, 3262 |
| BstNSI | RCATGY | 5 cut(s) | 127, 880, 2087, 2218, 3228 |
| BstSCI | CCNGG | 7 cut(s) | 6, 623, 731, 1605, 1989, 2102, 3260 |
| BstSFI | CTRYAG | 5 cut(s) | 1437, 1542, 1670, 2712, 3075 |
| BstSLI | GKGCMC | 2 cut(s) | 293, 2593 |
| BstUI | CGCG | 1 cut(s) | 1777 |
| BstV1I | GCAGC | 5 cut(s) | 488, 885, 2698, 3089, 3215 |
| BstV2I | GAAGAC | 7 cut(s) | 1116, 1278, 1467, 1750, 2339, 2445, 3171 |
| BstX2I | RGATCY | 3 cut(s) | 1144, 2115, 3062 |
| BstYI | RGATCY | 3 cut(s) | 1144, 2115, 3062 |
| Bsu15I | ATCGAT | 1 cut(s) | 1815 |
| BsuTUI | ATCGAT | 1 cut(s) | 1815 |
| BtgI | CCRYGG | 4 cut(s) | 829, 1823, 1934, 2610 |
| BtsCI | GGATG | 8 cut(s) | 798, 850, 1164, 2044, 2098, 2611, 2963, 3043 |
| BtsI | GCAGTG | 3 cut(s) | 291, 1275, 2379 |
| BtsIMutI | CAGTG | 7 cut(s) | 291, 364, 509, 528, 1275, 2379, 2851 |
| BveI | ACCTGC | 1 cut(s) | 2251 |
| CciI | TCATGA | 1 cut(s) | 3213 |
| CfoI | GCGC | 4 cut(s) | 528, 1129, 1777, 2631 |
| Cfr10I | RCCGGY | 4 cut(s) | 225, 619, 2027, 2881 |
| Cfr13I | GGNCC | 6 cut(s) | 4, 131, 247, 267, 825, 2483 |
| ClaI | ATCGAT | 1 cut(s) | 1815 |
| CseI | GACGC | 2 cut(s) | 443, 745 |
| CspAI | ACCGGT | 1 cut(s) | 225 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 3180, 3215 |
| DpnI | GATC | 9 cut(s) | 1146, 1548, 2117, 2303, 2745, 3064, 3102, 3132, 3273 |
| DpnII | GATC | 9 cut(s) | 1144, 1546, 2115, 2301, 2743, 3062, 3100, 3130, 3271 |
| DraI | TTTAAA | 2 cut(s) | 912, 2698 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 742 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 742 |
| EaeI | YGGCCR | 2 cut(s) | 621, 2848 |
| Eam1104I | CTCTTC | 5 cut(s) | 144, 285, 1096, 1120, 2966 |
| EarI | CTCTTC | 5 cut(s) | 144, 285, 1096, 1120, 2966 |
| Ecl136II | GAGCTC | 1 cut(s) | 2570 |
| Eco130I | CCWWGG | 3 cut(s) | 1409, 1823, 1924 |
| Eco147I | AGGCCT | 1 cut(s) | 541 |
| Eco24I | GRGCYC | 1 cut(s) | 2572 |
| Eco32I | GATATC | 2 cut(s) | 176, 2197 |
| Eco53kI | GAGCTC | 1 cut(s) | 2570 |
| Eco57I | CTGAAG | 5 cut(s) | 168, 1185, 1236, 2358, 3117 |
| Eco88I | CYCGRG | 1 cut(s) | 284 |
| EcoICRI | GAGCTC | 1 cut(s) | 2570 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 2971 |
| EcoO109I | RGGNCCY | 4 cut(s) | 131, 247, 267, 825 |
| EcoRI | GAATTC | 4 cut(s) | 1338, 1528, 2683, 2994 |
| EcoRII | CCWGG | 5 cut(s) | 623, 731, 1605, 2102, 3260 |
| EcoRV | GATATC | 2 cut(s) | 176, 2197 |
| EcoT14I | CCWWGG | 3 cut(s) | 1409, 1823, 1924 |
| EcoT38I | GRGCYC | 1 cut(s) | 2572 |
| ErhI | CCWWGG | 3 cut(s) | 1409, 1823, 1924 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1962, 1994 |
| FaqI | GGGAC | 1 cut(s) | 557 |
| FauNDI | CATATG | 1 cut(s) | 1684 |
| FbaI | TGATCA | 1 cut(s) | 2743 |
| FblI | GTMKAC | 3 cut(s) | 1328, 1436, 1636 |
| Fnu4HI | GCNGC | 6 cut(s) | 502, 874, 2219, 2712, 3078, 3204 |
| FokI | GGATG | 8 cut(s) | 805, 857, 1171, 2051, 2085, 2618, 2950, 3050 |
| FriOI | GRGCYC | 1 cut(s) | 2572 |
| Fsp4HI | GCNGC | 6 cut(s) | 502, 874, 2219, 2712, 3078, 3204 |
| FspBI | CTAG | 7 cut(s) | 66, 1029, 2109, 2183, 2499, 3285, 3368 |
| GlaI | GCGC | 4 cut(s) | 527, 1128, 1776, 2630 |
| GluI | GCNGC | 6 cut(s) | 502, 874, 2219, 2712, 3078, 3204 |
| GsuI | CTGGAG | 2 cut(s) | 376, 2827 |
| HapII | CCGG | 7 cut(s) | 8, 226, 620, 1990, 2028, 2829, 2882 |
| HgaI | GACGC | 2 cut(s) | 443, 745 |
| HhaI | GCGC | 4 cut(s) | 528, 1129, 1777, 2631 |
| Hin1I | GRCGYC | 2 cut(s) | 435, 737 |
| Hin6I | GCGC | 4 cut(s) | 526, 1127, 1775, 2629 |
| HinP1I | GCGC | 4 cut(s) | 526, 1127, 1775, 2629 |
| HincII | GTYRAC | 7 cut(s) | 664, 693, 2143, 2275, 2583, 3023, 3108 |
| HindII | GTYRAC | 7 cut(s) | 664, 693, 2143, 2275, 2583, 3023, 3108 |
| HindIII | AAGCTT | 2 cut(s) | 407, 865 |
| HpaI | GTTAAC | 1 cut(s) | 664 |
| HpaII | CCGG | 7 cut(s) | 8, 226, 620, 1990, 2028, 2829, 2882 |
| HphI | GGTGA | 5 cut(s) | 215, 609, 1459, 1525, 3237 |
| HpyCH4III | ACNGT | 8 cut(s) | 359, 513, 1326, 1473, 1585, 2727, 3146, 3240 |
| HpyCH4IV | ACGT | 1 cut(s) | 1863 |
| HpySE526I | ACGT | 1 cut(s) | 1863 |
| Hsp92I | GRCGYC | 2 cut(s) | 435, 737 |
| HspAI | GCGC | 4 cut(s) | 526, 1127, 1775, 2629 |
| Ksp22I | TGATCA | 1 cut(s) | 2743 |
| KspAI | GTTAAC | 1 cut(s) | 664 |
| Kzo9I | GATC | 9 cut(s) | 1144, 1546, 2115, 2301, 2743, 3062, 3100, 3130, 3271 |
| LguI | GCTCTTC | 1 cut(s) | 144 |
| LmnI | GCTCC | 2 cut(s) | 2796, 3085 |
| Lsp1109I | GCAGC | 5 cut(s) | 488, 885, 2698, 3089, 3215 |
| LweI | GCATC | 8 cut(s) | 370, 500, 1114, 1149, 1648, 2532, 2596, 3031 |
| MaeI | CTAG | 7 cut(s) | 66, 1029, 2109, 2183, 2499, 3285, 3368 |
| MaeII | ACGT | 1 cut(s) | 1863 |
| MaeIII | GTNAC | 7 cut(s) | 221, 326, 353, 507, 1255, 2837, 3280 |
| MalI | GATC | 9 cut(s) | 1146, 1548, 2117, 2303, 2745, 3064, 3102, 3132, 3273 |
| MboI | GATC | 9 cut(s) | 1144, 1546, 2115, 2301, 2743, 3062, 3100, 3130, 3271 |
| MfeI | CAATTG | 2 cut(s) | 486, 1753 |
| MflI | RGATCY | 3 cut(s) | 1144, 2115, 3062 |
| MhlI | GDGCHC | 3 cut(s) | 293, 2572, 2593 |
| MlsI | TGGCCA | 1 cut(s) | 2850 |
| MluNI | TGGCCA | 1 cut(s) | 2850 |
| MlyI | GAGTC | 6 cut(s) | 556, 1106, 1442, 2003, 2666, 2834 |
| MmeI | TCCRAC | 5 cut(s) | 1078, 1846, 2056, 2072, 3177 |
| Mox20I | TGGCCA | 1 cut(s) | 2850 |
| MroXI | GAANNNNTTC | 2 cut(s) | 1747, 2937 |
| MscI | TGGCCA | 1 cut(s) | 2850 |
| MslI | CAYNNNNRTG | 2 cut(s) | 165, 2895 |
| Msp20I | TGGCCA | 1 cut(s) | 2850 |
| MspA1I | CMGCKG | 2 cut(s) | 2221, 3251 |
| MspI | CCGG | 7 cut(s) | 8, 226, 620, 1990, 2028, 2829, 2882 |
| MspR9I | CCNGG | 7 cut(s) | 8, 625, 733, 1607, 1991, 2104, 3262 |
| MunI | CAATTG | 2 cut(s) | 486, 1753 |
| MvaI | CCWGG | 5 cut(s) | 625, 733, 1607, 2104, 3262 |
| MvnI | CGCG | 1 cut(s) | 1777 |
| NciI | CCSGG | 2 cut(s) | 8, 1991 |
| NcoI | CCATGG | 1 cut(s) | 1823 |
| NdeI | CATATG | 1 cut(s) | 1684 |
| NdeII | GATC | 9 cut(s) | 1144, 1546, 2115, 2301, 2743, 3062, 3100, 3130, 3271 |
| NlaIV | GGNNCC | 3 cut(s) | 6, 388, 827 |
| NmeAIII | GCCGAG | 2 cut(s) | 2041, 2684 |
| NmuCI | GTSAC | 6 cut(s) | 221, 326, 353, 507, 2837, 3280 |
| NspI | RCATGY | 5 cut(s) | 127, 880, 2087, 2218, 3228 |
| PaeI | GCATGC | 2 cut(s) | 880, 2218 |
| PagI | TCATGA | 1 cut(s) | 3213 |
| PceI | AGGCCT | 1 cut(s) | 541 |
| PciI | ACATGT | 3 cut(s) | 123, 2083, 3224 |
| PciSI | GCTCTTC | 1 cut(s) | 144 |
| PdmI | GAANNNNTTC | 2 cut(s) | 1747, 2937 |
| PfeI | GAWTC | 9 cut(s) | 534, 1184, 1246, 1732, 1858, 2224, 2818, 3217, 3348 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 3323 |
| PfoI | TCCNGGA | 1 cut(s) | 731 |
| PinAI | ACCGGT | 1 cut(s) | 225 |
| PkrI | GCNGC | 6 cut(s) | 503, 875, 2220, 2713, 3079, 3205 |
| PleI | GAGTC | 6 cut(s) | 556, 1106, 1441, 2002, 2666, 2834 |
| PpsI | GAGTC | 6 cut(s) | 556, 1106, 1441, 2002, 2666, 2834 |
| PscI | ACATGT | 3 cut(s) | 123, 2083, 3224 |
| PshBI | ATTAAT | 1 cut(s) | 2310 |
| PsiI | TTATAA | 1 cut(s) | 1913 |
| Psp124BI | GAGCTC | 1 cut(s) | 2572 |
| Psp6I | CCWGG | 5 cut(s) | 623, 731, 1605, 2102, 3260 |
| PspGI | CCWGG | 5 cut(s) | 623, 731, 1605, 2102, 3260 |
| PspN4I | GGNNCC | 3 cut(s) | 6, 388, 827 |
| PspPI | GGNCC | 6 cut(s) | 4, 131, 247, 267, 825, 2483 |
| PstI | CTGCAG | 3 cut(s) | 1674, 2716, 3079 |
| PsuI | RGATCY | 3 cut(s) | 1144, 2115, 3062 |
| PvuII | CAGCTG | 1 cut(s) | 3251 |
| RseI | CAYNNNNRTG | 2 cut(s) | 165, 2895 |
| SacI | GAGCTC | 1 cut(s) | 2572 |
| SapI | GCTCTTC | 1 cut(s) | 144 |
| SatI | GCNGC | 6 cut(s) | 502, 874, 2219, 2712, 3078, 3204 |
| Sau3AI | GATC | 9 cut(s) | 1144, 1546, 2115, 2301, 2743, 3062, 3100, 3130, 3271 |
| Sau96I | GGNCC | 6 cut(s) | 4, 131, 247, 267, 825, 2483 |
| ScaI | AGTACT | 4 cut(s) | 611, 763, 1853, 2718 |
| SchI | GAGTC | 6 cut(s) | 556, 1106, 1442, 2003, 2666, 2834 |
| ScrFI | CCNGG | 7 cut(s) | 8, 625, 733, 1607, 1991, 2104, 3262 |
| SduI | GDGCHC | 3 cut(s) | 293, 2572, 2593 |
| SfaNI | GCATC | 8 cut(s) | 370, 500, 1114, 1149, 1648, 2532, 2596, 3031 |
| SfcI | CTRYAG | 5 cut(s) | 1437, 1542, 1670, 2712, 3075 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 165, 2895 |
| SmlI | CTYRAG | 4 cut(s) | 1153, 1621, 1736, 2770 |
| SmoI | CTYRAG | 4 cut(s) | 1153, 1621, 1736, 2770 |
| SpeI | ACTAGT | 1 cut(s) | 3284 |
| SphI | GCATGC | 2 cut(s) | 880, 2218 |
| SseBI | AGGCCT | 1 cut(s) | 541 |
| SsiI | CCGC | 2 cut(s) | 1777, 2219 |
| SspMI | CTAG | 7 cut(s) | 66, 1029, 2109, 2183, 2499, 3285, 3368 |
| SstI | GAGCTC | 1 cut(s) | 2572 |
| StuI | AGGCCT | 1 cut(s) | 541 |
| StyD4I | CCNGG | 7 cut(s) | 6, 623, 731, 1605, 1989, 2102, 3260 |
| StyI | CCWWGG | 3 cut(s) | 1409, 1823, 1924 |
| TaaI | ACNGT | 8 cut(s) | 359, 513, 1326, 1473, 1585, 2727, 3146, 3240 |
| TaiI | ACGT | 1 cut(s) | 1866 |
| TaqI | TCGA | 3 cut(s) | 403, 1200, 1815 |
| TauI | GCSGC | 1 cut(s) | 2221 |
| TfiI | GAWTC | 9 cut(s) | 534, 1184, 1246, 1732, 1858, 2224, 2818, 3217, 3348 |
| TscAI | CASTG | 7 cut(s) | 298, 364, 516, 535, 1282, 2386, 2851 |
| TseFI | GTSAC | 6 cut(s) | 221, 326, 353, 507, 2837, 3280 |
| TseI | GCWGC | 5 cut(s) | 501, 873, 2711, 3077, 3203 |
| Tsp45I | GTSAC | 6 cut(s) | 221, 326, 353, 507, 2837, 3280 |
| TspGWI | ACGGA | 5 cut(s) | 209, 442, 1654, 1951, 2139 |
| TspRI | CASTG | 7 cut(s) | 298, 364, 516, 535, 1282, 2386, 2851 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 3323 |
| VneI | GTGCAC | 1 cut(s) | 2589 |
| VspI | ATTAAT | 1 cut(s) | 2310 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 2971 |
| XapI | RAATTY | 5 cut(s) | 1338, 1528, 2515, 2683, 2994 |
| XbaI | TCTAGA | 1 cut(s) | 2108 |
| XceI | RCATGY | 5 cut(s) | 127, 880, 2087, 2218, 3228 |
| XmiI | GTMKAC | 3 cut(s) | 1328, 1436, 1636 |
| XmnI | GAANNNNTTC | 2 cut(s) | 1747, 2937 |
| XspI | CTAG | 7 cut(s) | 66, 1029, 2109, 2183, 2499, 3285, 3368 |
| ZrmI | AGTACT | 4 cut(s) | 611, 763, 1853, 2718 |