pycom16g15470

E3 ubiquitin-protein ligase LUL4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0013528
Physical Location & Seq
Forward (+)
11407244 .. 11410845
3602 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1284 bp
ATGGGGATTTCATGGAGCACCAACAGAAGAAGGAACAACTATTACCAAAACCCACCACCACCTCCGCCGCCGCCGCCGCCACGTCAACCCCATTACATCTCAAGTTCTCATCCTTATTACTCATCGGAGCATGCCTCTCTTTCTCCACCTCCACCGCCACCACCTCCTCCTCCTCCACCGCCACCTGGCCCTTTGGTTTCAACTTCTTCTTCTTCCTCCTCCTTTTCATTTTCTTCTAATAACAACTACCCATATGCCAATTCCGCCACACCTTATGCCCCACAAGGACCGCAACCCAGAGCTCCGTACCCCACACACTCGCTCCCTCTCCCACCTCCTCCTCCTTCTCCGCCGCAAACCCATTACTGCTACGGCCACTACAACTCCTGCAACCATGGCAATTCCATGATGGGGCGCTTCAGTTACAATTACAATTACAATTACCAGCAGCCTTATAATTATGGTGCTACTCAGAGTAATGGGTGGCCCGCAATTCGGCCTCCTCCGCCGCCTCCGCCGCCTTACGTTGAGCATCAGAACGCCAAGAAGGTGAAGAACGATGTCAACGTGCATAAGGACACGTTGAGGCTTGAGATTGATGGCCTGAACCCTGATCAGCATTTGGTGTCTTTCGTTTTCGATGCCATGTATGATGGGAGCATTACGGTCTTCTACTTTGCCAAGGAAGAGCCAGACTGTAGGTTTGTTCCACTATTTCCTGAAGCATTTATGCCAGTGAGAGTCCCCTTTCAGAAGGGTCTTGGCCAAAAATTTCGCCAGCCCTCAGGAACAGGGATTGATTTAGGCTTCTTTGAGTTGGATGATCTTTCAAAACCATCACCAGGAGAAGATGTATTTCCGCTTGTAATATCTGCTGAAACATGTCTGCCACCTGATTCAACAGATGAGCATATTGTTGGGCCTACCCCAGATACATCCCCTCGTATGCAGATTACTCAAGCTGTGTTGGAAAAGAACAATGGTGACCATTTCCAAGTGAAAGTAATTAGGCAGATACTGTGGATTGATGAAGTTCGGTATGAGCTACGTGAGATATATGGAATAGGAAGCTCGACAGTGGAAAGCTTTGATGACACTGAACCAGGGAAGGAATGCGTGATATGCATGACTGAACCTAAGGACACTGCTGTGTTACCTTGTCGACATATGTGTATGTGCAGTGACTGTGCAAAAGAATTGCGGGTTCAATCCAATAAATGCCCCATCTGCCGTCAACCCATTGAGGAACTTATAGAGATCAAGATAAATAAAAGTGAGCAATGA

Protein Analysis

428

Amino Acids

47.85

Weight (kDa)

5.81

Isoelectric Point (pI)

71.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNF157-like_N PF26192 188 - 349 5.3e-70 RNF157-like N-terminal domain
zf-C3HC4_3 PF13920 370 - 416 9.3e-18 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 371 - 411 9.1e-08 Ring finger domain
Prok-RING_4 PF14447 372 - 415 2.5e-06 Prokaryotic RING finger family 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013528)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G06140
fragaria_vesca FvH4_4g14510
malus_domestica MD13G1183800.v1.1 MD16G1184200.v1.1
prunus_persica Prupe.1G151600_v2.0.a1 Prupe.1G151600_v2.0.a1
pyrus_communis pycom13g15860 pycom16g15470
rosa_chinensis RchiOBHm_Chr4g0418321
rosa_laevigata RLG00000007877
rosa_multiflora Rmu_ssc0000153.1_g000004
rosa_roxburghii Rroxscaffold_5G00361510
rosa_rugosa Rorug04G0152200
rosa_samantha Rh4AG213700 Rh4BG216700 Rh4CG225300 Rh4DG212200
rosa_wichuraiana Rw4G018150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 456
AccI GTMKAC 1 cut(s) 1162
AcoI YGGCCR 2 cut(s) 373, 763
AcsI RAATTY 1 cut(s) 770
AcuI CTGAAG 2 cut(s) 403, 741
AfaI GTAC 1 cut(s) 308
AfiI CCNNNNNNNGG 4 cut(s) 185, 411, 495, 842
AflIII ACRYGT 2 cut(s) 579, 881
AgsI TTSAA 4 cut(s) 201, 831, 900, 1208
AjiI CACGTC 1 cut(s) 83
AjnI CCWGG 3 cut(s) 184, 841, 1102
AleI CACNNNNGTG 1 cut(s) 1148
AluBI AGCT 5 cut(s) 302, 962, 1045, 1071, 1086
AluI AGCT 5 cut(s) 302, 962, 1045, 1071, 1086
Alw21I GWGCWC 2 cut(s) 20, 304
AlwNI CAGNNNCTG 2 cut(s) 1018, 1185
AoxI GGCC 7 cut(s) 187, 373, 485, 497, 601, 763, 920
ApeKI GCWGC 1 cut(s) 448
ApoI RAATTY 1 cut(s) 770
ArsI GACNNNNNNTTYG 2 cut(s) 686, 718
AspLEI GCGC 1 cut(s) 417
AspS9I GGNCC 4 cut(s) 188, 287, 486, 920
AsuHPI GGTGA 3 cut(s) 562, 831, 995
AvaII GGWCC 1 cut(s) 287
AxyI CCTNAGG 2 cut(s) 784, 1137
BalI TGGCCA 1 cut(s) 765
BanII GRGCYC 1 cut(s) 304
BbsI GAAGAC 1 cut(s) 661
Bbv12I GWGCWC 2 cut(s) 20, 304
BbvI GCAGC 1 cut(s) 460
BccI CCATC 5 cut(s) 403, 593, 647, 844, 1232
BceAI ACGGC 2 cut(s) 388, 1215
BciT130I CCWGG 3 cut(s) 186, 843, 1104
BclI TGATCA 1 cut(s) 613
BfmI CTRYAG 1 cut(s) 697
BfoI RGCGCY 1 cut(s) 418
BisI GCNGC 8 cut(s) 68, 71, 74, 77, 353, 449, 509, 518
BlsI GCNGC 8 cut(s) 69, 72, 75, 78, 354, 450, 510, 519
Bme1390I CCNGG 3 cut(s) 186, 843, 1104
Bme18I GGWCC 1 cut(s) 287
BmgBI CACGTC 1 cut(s) 83
BmgT120I GGNCC 4 cut(s) 188, 287, 486, 920
BmrFI CCNGG 3 cut(s) 186, 843, 1104
BmsI GCATC 2 cut(s) 541, 631
BpiI GAAGAC 1 cut(s) 661
BplI GAGNNNNNCTC 2 cut(s) 119, 151
BpuEI CTTGAG 3 cut(s) 85, 611, 942
BsaAI YACGTR 1 cut(s) 1049
BsaJI CCNNGG 3 cut(s) 394, 681, 1103
BsaXI ACNNNNNCTCC 6 cut(s) 306, 336, 368, 398, 837, 867
Bsc4I CCNNNNNNNGG 4 cut(s) 185, 411, 495, 842
Bse1I ACTGG 1 cut(s) 734
Bse21I CCTNAGG 2 cut(s) 784, 1137
BseBI CCWGG 3 cut(s) 186, 843, 1104
BseDI CCNNGG 3 cut(s) 394, 681, 1103
BseGI GGATG 3 cut(s) 109, 826, 935
BseLI CCNNNNNNNGG 4 cut(s) 185, 411, 495, 842
BseMII CTCAG 2 cut(s) 485, 798
BseNI ACTGG 1 cut(s) 734
BseRI GAGGAG 7 cut(s) 156, 159, 162, 208, 327, 330, 492
BseXI GCAGC 1 cut(s) 460
BsgI GTGCAG 1 cut(s) 1198
BshFI GGCC 7 cut(s) 189, 375, 487, 499, 603, 765, 922
BsiHKAI GWGCWC 2 cut(s) 20, 304
BslFI GGGAC 1 cut(s) 728
BslI CCNNNNNNNGG 4 cut(s) 185, 411, 495, 842
BsmFI GGGAC 1 cut(s) 728
BsmI GAATGC 1 cut(s) 1118
BsnI GGCC 7 cut(s) 189, 375, 487, 499, 603, 765, 922
Bsp1286I GDGCHC 2 cut(s) 20, 304
Bsp143I GATC 3 cut(s) 613, 823, 1257
Bsp19I CCATGG 1 cut(s) 394
BspANI GGCC 7 cut(s) 189, 375, 487, 499, 603, 765, 922
BspCNI CTCAG 2 cut(s) 484, 797
BspQI GCTCTTC 1 cut(s) 681
BsrI ACTGG 1 cut(s) 734
BssECI CCNNGG 3 cut(s) 394, 681, 1103
BssMI GATC 3 cut(s) 613, 823, 1257
BssT1I CCWWGG 2 cut(s) 394, 681
Bst2UI CCWGG 3 cut(s) 186, 843, 1104
Bst4CI ACNGT 5 cut(s) 667, 698, 1020, 1078, 1187
Bst6I CTCTTC 1 cut(s) 681
BstBAI YACGTR 1 cut(s) 1049
BstC8I GCNNGC 3 cut(s) 132, 489, 779
BstDEI CTNAG 3 cut(s) 471, 784, 1137
BstDSI CCRYGG 1 cut(s) 394
BstEII GGTNACC 1 cut(s) 983
BstF5I GGATG 3 cut(s) 109, 826, 935
BstH2I RGCGCY 1 cut(s) 418
BstHHI GCGC 1 cut(s) 417
BstKTI GATC 3 cut(s) 616, 826, 1260
BstMBI GATC 3 cut(s) 613, 823, 1257
BstMWI GCNNNNNNNGC 9 cut(s) 73, 76, 263, 396, 505, 514, 517, 1122, 1227
BstNI CCWGG 3 cut(s) 186, 843, 1104
BstNSI RCATGY 2 cut(s) 134, 885
BstPI GGTNACC 1 cut(s) 983
BstSCI CCNGG 3 cut(s) 184, 841, 1102
BstSFI CTRYAG 1 cut(s) 697
BstV1I GCAGC 1 cut(s) 460
BstV2I GAAGAC 1 cut(s) 661
Bsu36I CCTNAGG 2 cut(s) 784, 1137
BsuRI GGCC 7 cut(s) 189, 375, 487, 499, 603, 765, 922
BtgI CCRYGG 1 cut(s) 394
BtrI CACGTC 1 cut(s) 83
BtsCI GGATG 3 cut(s) 109, 826, 935
BtsI GCAGTG 2 cut(s) 1143, 1186
BtsIMutI CAGTG 5 cut(s) 741, 1083, 1095, 1143, 1186
Cac8I GCNNGC 3 cut(s) 132, 489, 779
CaiI CAGNNNCTG 2 cut(s) 1018, 1185
CfoI GCGC 1 cut(s) 417
Cfr13I GGNCC 4 cut(s) 188, 287, 486, 920
Csp6I GTAC 1 cut(s) 307
CviAII CATG 7 cut(s) 12, 131, 395, 406, 646, 882, 1126
CviQI GTAC 1 cut(s) 307
DdeI CTNAG 3 cut(s) 471, 784, 1137
DpnI GATC 3 cut(s) 615, 825, 1259
DpnII GATC 3 cut(s) 613, 823, 1257
EaeI YGGCCR 2 cut(s) 373, 763
Eam1104I CTCTTC 1 cut(s) 681
EarI CTCTTC 1 cut(s) 681
EciI GGCGGA 5 cut(s) 54, 253, 339, 495, 504
Ecl136II GAGCTC 1 cut(s) 302
Eco130I CCWWGG 2 cut(s) 394, 681
Eco24I GRGCYC 1 cut(s) 304
Eco47I GGWCC 1 cut(s) 287
Eco53kI GAGCTC 1 cut(s) 302
Eco57I CTGAAG 2 cut(s) 403, 741
Eco81I CCTNAGG 2 cut(s) 784, 1137
Eco91I GGTNACC 1 cut(s) 983
EcoICRI GAGCTC 1 cut(s) 302
EcoO65I GGTNACC 1 cut(s) 983
EcoRII CCWGG 3 cut(s) 184, 841, 1102
EcoT14I CCWWGG 2 cut(s) 394, 681
EcoT22I ATGCAT 1 cut(s) 1127
EcoT38I GRGCYC 1 cut(s) 304
ErhI CCWWGG 2 cut(s) 394, 681
FaeI CATG 7 cut(s) 15, 134, 398, 409, 649, 885, 1129
FaqI GGGAC 1 cut(s) 728
FatI CATG 7 cut(s) 11, 130, 394, 405, 645, 881, 1125
FauI CCCGC 2 cut(s) 496, 1194
FauNDI CATATG 2 cut(s) 253, 1167
FbaI TGATCA 1 cut(s) 613
FblI GTMKAC 1 cut(s) 1162
Fnu4HI GCNGC 8 cut(s) 68, 71, 74, 77, 353, 449, 509, 518
FokI GGATG 3 cut(s) 96, 833, 922
FriOI GRGCYC 1 cut(s) 304
Fsp4HI GCNGC 8 cut(s) 68, 71, 74, 77, 353, 449, 509, 518
GlaI GCGC 1 cut(s) 416
GluI GCNGC 8 cut(s) 68, 71, 74, 77, 353, 449, 509, 518
HaeII RGCGCY 1 cut(s) 418
HaeIII GGCC 7 cut(s) 189, 375, 487, 499, 603, 765, 922
HhaI GCGC 1 cut(s) 417
Hin1II CATG 7 cut(s) 15, 134, 398, 409, 649, 885, 1129
Hin6I GCGC 1 cut(s) 415
HinP1I GCGC 1 cut(s) 415
HincII GTYRAC 4 cut(s) 86, 565, 1163, 1235
HindII GTYRAC 4 cut(s) 86, 565, 1163, 1235
HindIII AAGCTT 1 cut(s) 1084
HinfI GANTC 2 cut(s) 741, 896
HphI GGTGA 3 cut(s) 562, 831, 995
Hpy166II GTNNAC 4 cut(s) 86, 565, 1163, 1235
Hpy188I TCNGA 4 cut(s) 127, 474, 537, 753
Hpy188III TCNNGA 3 cut(s) 719, 786, 1261
Hpy8I GTNNAC 4 cut(s) 86, 565, 1163, 1235
HpyAV CCTTC 5 cut(s) 24, 354, 541, 748, 1102
HpyCH4III ACNGT 5 cut(s) 667, 698, 1020, 1078, 1187
HpyCH4IV ACGT 5 cut(s) 82, 525, 567, 581, 1048
HpyCH4V TGCA 6 cut(s) 390, 571, 949, 1125, 1179, 1190
HpyF10VI GCNNNNNNNGC 9 cut(s) 73, 76, 263, 396, 505, 514, 517, 1122, 1227
HpyF3I CTNAG 3 cut(s) 471, 784, 1137
HpySE526I ACGT 5 cut(s) 82, 525, 567, 581, 1048
Hsp92II CATG 7 cut(s) 15, 134, 398, 409, 649, 885, 1129
HspAI GCGC 1 cut(s) 415
Ksp22I TGATCA 1 cut(s) 613
Kzo9I GATC 3 cut(s) 613, 823, 1257
LguI GCTCTTC 1 cut(s) 681
LmnI GCTCC 5 cut(s) 15, 127, 307, 327, 657
Lsp1109I GCAGC 1 cut(s) 460
LweI GCATC 2 cut(s) 541, 631
MaeII ACGT 5 cut(s) 82, 525, 567, 581, 1048
MaeIII GTNAC 4 cut(s) 422, 983, 1152, 1181
MalI GATC 3 cut(s) 615, 825, 1259
MboI GATC 3 cut(s) 613, 823, 1257
MboII GAAGA 9 cut(s) 39, 198, 201, 204, 225, 565, 661, 698, 860
MhlI GDGCHC 2 cut(s) 20, 304
MlsI TGGCCA 1 cut(s) 765
MluNI TGGCCA 1 cut(s) 765
MlyI GAGTC 1 cut(s) 750
MmeI TCCRAC 2 cut(s) 798, 948
Mox20I TGGCCA 1 cut(s) 765
Mph1103I ATGCAT 1 cut(s) 1127
MscI TGGCCA 1 cut(s) 765
MslI CAYNNNNRTG 1 cut(s) 1148
Msp20I TGGCCA 1 cut(s) 765
MspR9I CCNGG 3 cut(s) 186, 843, 1104
Mva1269I GAATGC 1 cut(s) 1118
MvaI CCWGG 3 cut(s) 186, 843, 1104
MwoI GCNNNNNNNGC 9 cut(s) 73, 76, 263, 396, 505, 514, 517, 1122, 1227
NcoI CCATGG 1 cut(s) 394
NdeI CATATG 2 cut(s) 253, 1167
NdeII GATC 3 cut(s) 613, 823, 1257
NlaIII CATG 7 cut(s) 15, 134, 398, 409, 649, 885, 1129
NmuCI GTSAC 2 cut(s) 983, 1181
NsiI ATGCAT 1 cut(s) 1127
NspI RCATGY 2 cut(s) 134, 885
OliI CACNNNNGTG 1 cut(s) 1148
PaeI GCATGC 1 cut(s) 134
PciI ACATGT 1 cut(s) 881
PciSI GCTCTTC 1 cut(s) 681
PcsI WCGNNNNNNNCGW 1 cut(s) 564
PctI GAATGC 1 cut(s) 1118
PfeI GAWTC 1 cut(s) 896
PkrI GCNGC 8 cut(s) 69, 72, 75, 78, 354, 450, 510, 519
PleI GAGTC 1 cut(s) 749
PpsI GAGTC 1 cut(s) 749
Ppu21I YACGTR 1 cut(s) 1049
PscI ACATGT 1 cut(s) 881
PsiI TTATAA 1 cut(s) 456
Psp124BI GAGCTC 1 cut(s) 304
Psp6I CCWGG 3 cut(s) 184, 841, 1102
PspEI GGTNACC 1 cut(s) 983
PspGI CCWGG 3 cut(s) 184, 841, 1102
PspPI GGNCC 4 cut(s) 188, 287, 486, 920
PsrI GAACNNNNNNTAC 2 cut(s) 26, 58
PstNI CAGNNNCTG 2 cut(s) 1018, 1185
RsaI GTAC 1 cut(s) 308
RsaNI GTAC 1 cut(s) 307
RseI CAYNNNNRTG 1 cut(s) 1148
SacI GAGCTC 1 cut(s) 304
SalI GTCGAC 1 cut(s) 1161
SapI GCTCTTC 1 cut(s) 681
SatI GCNGC 8 cut(s) 68, 71, 74, 77, 353, 449, 509, 518
Sau3AI GATC 3 cut(s) 613, 823, 1257
Sau96I GGNCC 4 cut(s) 188, 287, 486, 920
SchI GAGTC 1 cut(s) 750
ScrFI CCNGG 3 cut(s) 186, 843, 1104
SduI GDGCHC 2 cut(s) 20, 304
SfaNI GCATC 2 cut(s) 541, 631
SfcI CTRYAG 1 cut(s) 697
SinI GGWCC 1 cut(s) 287
SmiMI CAYNNNNRTG 1 cut(s) 1148
SmlI CTYRAG 3 cut(s) 100, 590, 957
SmoI CTYRAG 3 cut(s) 100, 590, 957
SphI GCATGC 1 cut(s) 134
SstI GAGCTC 1 cut(s) 304
StyD4I CCNGG 3 cut(s) 184, 841, 1102
StyI CCWWGG 2 cut(s) 394, 681
TaaI ACNGT 5 cut(s) 667, 698, 1020, 1078, 1187
TaiI ACGT 5 cut(s) 85, 528, 570, 584, 1051
TaqI TCGA 3 cut(s) 639, 1073, 1162
TauI GCSGC 7 cut(s) 70, 73, 76, 79, 355, 511, 520
TfiI GAWTC 1 cut(s) 896
TscAI CASTG 5 cut(s) 741, 1083, 1102, 1150, 1186
TseFI GTSAC 2 cut(s) 983, 1181
TseI GCWGC 1 cut(s) 448
Tsp45I GTSAC 2 cut(s) 983, 1181
TspDTI ATGAA 2 cut(s) 216, 1044
TspGWI ACGGA 1 cut(s) 294
TspRI CASTG 5 cut(s) 741, 1083, 1102, 1150, 1186
VpaK11BI GGWCC 1 cut(s) 287
XapI RAATTY 1 cut(s) 770
XceI RCATGY 2 cut(s) 134, 885
XmiI GTMKAC 1 cut(s) 1162
Zsp2I ATGCAT 1 cut(s) 1127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.