pycom16g18560

MazG nucleotide pyrophosphohydrolase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0003799
Physical Location & Seq
Forward (+)
15239679 .. 15241196
1518 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 369 bp
ATGGGAGAAGGAGAAGAGATTAATGCAGCAGAAGAAGAAAGAGAGAATATCAGTCTCAAGGACCTCTCTAAGAAGCTTGAGGAATTTGCCAAGGCTAGAGATTGGGAGAAATACCACAGTCCCAGAAACCTACTCCTGGCTATGGTGGGAGAGGTAGGAGAACTATCAGAAATCTTCCAGTGGAGGGGAGAGGTGGACAGAGGGTTGCCAAATTGGAAGGAATCAGATAAAGAGCATCTGGGAGAAGAGCTTTCTGATGTGCTTCTTTACCTCATCAGGTTGGCGGATATATGCGGCATTGACCTTGCTGATGCTGCCTCCAAAAAGATTGTCAAGAATGCCATCAAATACCCTCCCAAATTCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00240 ko01100 map00240 map01100
Pfam Domains
Protein Families
MazG MazG-like

Protein Analysis

123

Amino Acids

13.98

Weight (kDa)

4.86

Isoelectric Point (pI)

26.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MazG PF03819 40 - 113 4.8e-07 MazG nucleotide pyrophosphohydrolase domain
MazG-like PF12643 51 - 118 2.1e-07 MazG-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 284, 294
AcsI RAATTY 2 cut(s) 83, 359
AfiI CCNNNNNNNGG 3 cut(s) 136, 142, 184
AjnI CCWGG 1 cut(s) 135
AluBI AGCT 2 cut(s) 76, 250
AluI AGCT 2 cut(s) 76, 250
Alw26I GTCTC 1 cut(s) 59
ApeKI GCWGC 2 cut(s) 26, 314
ApoI RAATTY 2 cut(s) 83, 359
ArsI GACNNNNNNTTYG 2 cut(s) 315, 347
AseI ATTAAT 1 cut(s) 21
AspS9I GGNCC 1 cut(s) 61
AvaII GGWCC 1 cut(s) 61
BbvI GCAGC 2 cut(s) 38, 301
BccI CCATC 1 cut(s) 350
BciT130I CCWGG 1 cut(s) 137
BcoDI GTCTC 1 cut(s) 59
BfaI CTAG 1 cut(s) 96
BisI GCNGC 3 cut(s) 27, 295, 315
BlsI GCNGC 3 cut(s) 28, 296, 316
Bme1390I CCNGG 1 cut(s) 137
Bme18I GGWCC 1 cut(s) 61
BmgT120I GGNCC 1 cut(s) 61
BmrFI CCNGG 1 cut(s) 137
BmsI GCATC 2 cut(s) 244, 301
BpuEI CTTGAG 2 cut(s) 41, 98
BsaJI CCNNGG 1 cut(s) 90
Bsc4I CCNNNNNNNGG 3 cut(s) 136, 142, 184
Bse1I ACTGG 1 cut(s) 178
BseBI CCWGG 1 cut(s) 137
BseDI CCNNGG 1 cut(s) 90
BseLI CCNNNNNNNGG 3 cut(s) 136, 142, 184
BseNI ACTGG 1 cut(s) 178
BseXI GCAGC 2 cut(s) 38, 301
BslFI GGGAC 1 cut(s) 105
BslI CCNNNNNNNGG 3 cut(s) 136, 142, 184
BsmAI GTCTC 1 cut(s) 59
BsmFI GGGAC 1 cut(s) 105
BsmI GAATGC 1 cut(s) 343
BspACI CCGC 2 cut(s) 284, 294
BspQI GCTCTTC 1 cut(s) 240
BsrI ACTGG 1 cut(s) 178
BssECI CCNNGG 1 cut(s) 90
BssT1I CCWWGG 1 cut(s) 90
Bst2UI CCWGG 1 cut(s) 137
Bst4CI ACNGT 1 cut(s) 119
Bst6I CTCTTC 2 cut(s) 9, 240
BstDEI CTNAG 1 cut(s) 69
BstMAI GTCTC 1 cut(s) 59
BstMWI GCNNNNNNNGC 1 cut(s) 314
BstNI CCWGG 1 cut(s) 137
BstSCI CCNGG 1 cut(s) 135
BstV1I GCAGC 2 cut(s) 38, 301
BtsIMutI CAGTG 1 cut(s) 185
Cfr13I GGNCC 1 cut(s) 61
CviJI RGCY 4 cut(s) 76, 95, 140, 250
CviKI_1 RGCY 4 cut(s) 76, 95, 140, 250
DdeI CTNAG 1 cut(s) 69
Eam1104I CTCTTC 2 cut(s) 9, 240
EarI CTCTTC 2 cut(s) 9, 240
EciI GGCGGA 1 cut(s) 299
Eco130I CCWWGG 1 cut(s) 90
Eco47I GGWCC 1 cut(s) 61
EcoO109I RGGNCCY 1 cut(s) 61
EcoRII CCWGG 1 cut(s) 135
EcoT14I CCWWGG 1 cut(s) 90
ErhI CCWWGG 1 cut(s) 90
FaiI YATR 3 cut(s) 143, 290, 292
FaqI GGGAC 1 cut(s) 105
Fnu4HI GCNGC 3 cut(s) 27, 295, 315
Fsp4HI GCNGC 3 cut(s) 27, 295, 315
FspBI CTAG 1 cut(s) 96
GluI GCNGC 3 cut(s) 27, 295, 315
HindIII AAGCTT 1 cut(s) 74
HinfI GANTC 1 cut(s) 221
Hpy166II GTNNAC 1 cut(s) 196
Hpy188I TCNGA 3 cut(s) 169, 226, 256
Hpy188III TCNNGA 1 cut(s) 334
Hpy8I GTNNAC 1 cut(s) 196
HpyAV CCTTC 1 cut(s) 211
HpyCH4III ACNGT 1 cut(s) 119
HpyCH4V TGCA 1 cut(s) 26
HpyF10VI GCNNNNNNNGC 1 cut(s) 314
HpyF3I CTNAG 1 cut(s) 69
LguI GCTCTTC 1 cut(s) 240
LpnPI CCDG 6 cut(s) 122, 136, 149, 191, 224, 262
Lsp1109I GCAGC 2 cut(s) 38, 301
LweI GCATC 2 cut(s) 244, 301
MaeI CTAG 1 cut(s) 96
MboII GAAGA 6 cut(s) 26, 44, 47, 166, 257, 355
MluCI AATT 3 cut(s) 83, 211, 359
MnlI CCTC 9 cut(s) 73, 74, 145, 177, 184, 194, 281, 328, 363
MseI TTAA 1 cut(s) 21
MspR9I CCNGG 1 cut(s) 137
Mva1269I GAATGC 1 cut(s) 343
MvaI CCWGG 1 cut(s) 137
MwoI GCNNNNNNNGC 1 cut(s) 314
PciSI GCTCTTC 1 cut(s) 240
PctI GAATGC 1 cut(s) 343
PfeI GAWTC 1 cut(s) 221
PkrI GCNGC 3 cut(s) 28, 296, 316
PpuMI RGGWCCY 1 cut(s) 61
PshBI ATTAAT 1 cut(s) 21
Psp5II RGGWCCY 1 cut(s) 61
Psp6I CCWGG 1 cut(s) 135
PspGI CCWGG 1 cut(s) 135
PspPI GGNCC 1 cut(s) 61
PspPPI RGGWCCY 1 cut(s) 61
SapI GCTCTTC 1 cut(s) 240
SaqAI TTAA 1 cut(s) 21
SatI GCNGC 3 cut(s) 27, 295, 315
Sau96I GGNCC 1 cut(s) 61
ScrFI CCNGG 1 cut(s) 137
SetI ASST 9 cut(s) 66, 78, 132, 156, 195, 252, 273, 281, 306
SfaNI GCATC 2 cut(s) 244, 301
SinI GGWCC 1 cut(s) 61
SmlI CTYRAG 2 cut(s) 56, 77
SmoI CTYRAG 2 cut(s) 56, 77
Sse9I AATT 3 cut(s) 83, 211, 359
SsiI CCGC 2 cut(s) 284, 294
SspMI CTAG 1 cut(s) 96
StyD4I CCNGG 1 cut(s) 135
StyI CCWWGG 1 cut(s) 90
TaaI ACNGT 1 cut(s) 119
TasI AATT 3 cut(s) 83, 211, 359
TauI GCSGC 1 cut(s) 297
TfiI GAWTC 1 cut(s) 221
Tru1I TTAA 1 cut(s) 21
Tru9I TTAA 1 cut(s) 21
TscAI CASTG 1 cut(s) 185
TseI GCWGC 2 cut(s) 26, 314
TspRI CASTG 1 cut(s) 185
VpaK11BI GGWCC 1 cut(s) 61
VspI ATTAAT 1 cut(s) 21
XapI RAATTY 2 cut(s) 83, 359
XspI CTAG 1 cut(s) 96
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.