pycom16g23520

Putative serine esterase (DUF676)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0007465
Physical Location & Seq
Reverse (-)
24579486 .. 24583726
4241 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1302 bp
ATGATCGCGGCCAATCCTCCGCTCAATTCCGCAATCCCCGTCCTCCGTTCCGCCAACCCGACCCGAAAGCATCGCTGTTTCGAGGCGCTGTGCTCCAGCAACCACCTAGGCTTTAGCTCCTCCAAGCGTGGGCTGTGGGTCACTCGGACTCGGATGGACTTGCTCCGCCGATTTGTAGCCGGAGGATGCTTCCGGGACCCCGGTCACCAGGGGATTAAGGTGGAGATCGACAGCGGCGGGGAAGATGTCTTTGCCGCCAACCCCAAGGGCAGCGCCGCCCCGGAACATCTTGTCATTATGGTCAATGGGATCATCGGCAGTGCTTCAGACTGGAGATACGCTGCAGATCAGTTTGTGAAAAAGCTTCCTGATAAAGTAGTTGTGCACCGTAGTGAATGTAACTCTTCAAGATTGACATTTGATGGTGTTGACTTGATGGGTGAGAGGCTAGCTGAAGAGGTATTAGCTGTCATCAGAAAGAGGTCTGAGGTACGGAAAATATCCTTTGTGGCTCATTCCTTAGGTGGGTTGGTTGCAAGGTATGCCATCGGAAGGCTGTATGAACCTTTTCCAAAATCAGAACCCTCGGGTCAAAATGGTTATTGCGTAATTGATGGGCATACAAATAATTTGACACAATCCATTGACCAGCCTCACCATGGTACAATTGCTGGATTGGAAGCAATGAACTTTATAACGGTCGCAACACCACATCTTGGTTCAAGGGGAAATAAACAGCTCCCATTTCTCTGTGGTCTTCCTTTTCTCGAGAGAAGAGCTTCTCAAACTGCGCACTTTATTGCTGGGAGATCTGATAAGCATCTCTTCCTAACAGACGATGATGATGGCAGACCTCCTCTTCTTCTTCGAATGGTTAATGACTGTGATGACCTAAAATTCATATCAGCATTACGTGCATTTAAGCGTCGTGTGGCATATGCTAATGTAAATTATGATTATATGGTTGGGTGGAGAACATCATCAATCCGGCGTCAATATGAACTTCCAAAGTCCAACCTCCTTGTAACCGATGAGAGATATCCACATATCGTATATATCAAGCGAGAAACTTCTGATCATATTCATAGTAAAGCATCATCAAGTGCTCAAGACGTAAACATAGACTTGGAAGAGGAGATGATTAGAGGTCTTACTCAGATACCTTGGGAACGCGTGGATGTTAGCTTTAAGAAAAGCACACAACGATATATTGCTCATAATACCATTCAGGTGAAGAGTTATTGGTTCAATTCTGATGGTGCGGATGTGGTTTTCCATATGATAGATTGCTTCCTTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF676

Protein Analysis

434

Amino Acids

48.65

Weight (kDa)

8.91

Isoelectric Point (pI)

42.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF676 PF05057 94 - 326 6.8e-67 Putative serine esterase (DUF676)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 695
Acc16I TGCGCA 1 cut(s) 792
AccB7I CCANNNNNTGG 1 cut(s) 716
AccBSI CCGCTC 1 cut(s) 22
AccII CGCG 2 cut(s) 8, 1173
AclWI GGATC 1 cut(s) 317
AcoI YGGCCR 1 cut(s) 9
AcsI RAATTY 1 cut(s) 896
AcuI CTGAAG 2 cut(s) 309, 474
AcyI GRCGYC 1 cut(s) 991
AfaI GTAC 2 cut(s) 492, 664
AfiI CCNNNNNNNGG 5 cut(s) 129, 525, 552, 659, 716
AflIII ACRYGT 1 cut(s) 1171
AgsI TTSAA 3 cut(s) 408, 723, 1249
AjnI CCWGG 1 cut(s) 207
AleI CACNNNNGTG 1 cut(s) 390
AluBI AGCT 7 cut(s) 117, 364, 452, 467, 739, 779, 1185
AluI AGCT 7 cut(s) 117, 364, 452, 467, 739, 779, 1185
Alw21I GWGCWC 3 cut(s) 95, 387, 1108
Alw44I GTGCAC 1 cut(s) 383
AlwI GGATC 1 cut(s) 317
Ama87I CYCGRG 2 cut(s) 586, 767
AoxI GGCC 1 cut(s) 9
ApaLI GTGCAC 1 cut(s) 383
ApeKI GCWGC 2 cut(s) 270, 341
ApoI RAATTY 1 cut(s) 896
Asp700I GAANNNNTTC 2 cut(s) 567, 778
AspA2I CCTAGG 1 cut(s) 106
AspLEI GCGC 3 cut(s) 88, 275, 793
AspS9I GGNCC 1 cut(s) 196
AsuC2I CCSGG 3 cut(s) 194, 201, 281
AsuHPI GGTGA 4 cut(s) 197, 452, 647, 1243
AsuII TTCGAA 1 cut(s) 868
AsuNHI GCTAGC 1 cut(s) 448
AvaI CYCGRG 2 cut(s) 586, 767
AvaII GGWCC 1 cut(s) 196
AvrII CCTAGG 1 cut(s) 106
AxyI CCTNAGG 1 cut(s) 520
BaeGI GKGCMC 1 cut(s) 387
BbsI GAAGAC 1 cut(s) 749
Bbv12I GWGCWC 3 cut(s) 95, 387, 1108
BbvI GCAGC 2 cut(s) 282, 328
BccI CCATC 7 cut(s) 148, 416, 430, 554, 608, 839, 1250
BciT130I CCWGG 1 cut(s) 209
BclI TGATCA 1 cut(s) 1075
BcnI CCSGG 3 cut(s) 194, 201, 281
BfaI CTAG 2 cut(s) 107, 449
BfmI CTRYAG 1 cut(s) 342
BfoI RGCGCY 2 cut(s) 89, 276
BglII AGATCT 1 cut(s) 809
BisI GCNGC 6 cut(s) 9, 235, 255, 271, 276, 342
BlnI CCTAGG 1 cut(s) 106
BlsI GCNGC 6 cut(s) 10, 236, 256, 272, 277, 343
Bme1390I CCNGG 4 cut(s) 194, 201, 209, 281
Bme18I GGWCC 1 cut(s) 196
BmeT110I CYCGRG 2 cut(s) 586, 767
BmgT120I GGNCC 1 cut(s) 196
BmiI GGNNCC 2 cut(s) 197, 198
BmrFI CCNGG 4 cut(s) 194, 201, 209, 281
BmsI GCATC 4 cut(s) 79, 176, 829, 1103
BmtI GCTAGC 1 cut(s) 452
BoxI GACNNNNGTC 1 cut(s) 201
BpiI GAAGAC 1 cut(s) 749
BpmI CTGGAG 2 cut(s) 79, 352
Bpu14I TTCGAA 1 cut(s) 868
BpuEI CTTGAG 1 cut(s) 1092
BpuMI CCSGG 3 cut(s) 194, 201, 281
BsaAI YACGTR 1 cut(s) 914
BsaBI GATNNNNATC 1 cut(s) 819
BsaHI GRCGYC 1 cut(s) 991
BsaJI CCNNGG 8 cut(s) 106, 199, 208, 264, 279, 585, 658, 1163
Bsc4I CCNNNNNNNGG 5 cut(s) 129, 525, 552, 659, 716
Bse1I ACTGG 1 cut(s) 335
Bse21I CCTNAGG 1 cut(s) 520
Bse3DI GCAATG 1 cut(s) 690
Bse8I GATNNNNATC 1 cut(s) 819
BseBI CCWGG 1 cut(s) 209
BseDI CCNNGG 8 cut(s) 106, 199, 208, 264, 279, 585, 658, 1163
BseGI GGATG 4 cut(s) 159, 191, 1183, 1270
BseJI GATNNNNATC 1 cut(s) 819
BseLI CCNNNNNNNGG 5 cut(s) 129, 525, 552, 659, 716
BseMI GCAATG 1 cut(s) 690
BseMII CTCAG 2 cut(s) 477, 1169
BseNI ACTGG 1 cut(s) 335
BseRI GAGGAG 3 cut(s) 109, 846, 1148
BseSI GKGCMC 1 cut(s) 387
BseXI GCAGC 2 cut(s) 282, 328
BseYI CCCAGC 1 cut(s) 803
Bsh1236I CGCG 2 cut(s) 8, 1173
Bsh1285I CGRYCG 1 cut(s) 702
BshFI GGCC 1 cut(s) 11
BsiEI CGRYCG 1 cut(s) 702
BsiHKAI GWGCWC 3 cut(s) 95, 387, 1108
BsiHKCI CYCGRG 2 cut(s) 586, 767
BsiSI CCGG 5 cut(s) 180, 193, 201, 281, 988
BslFI GGGAC 1 cut(s) 209
BslI CCNNNNNNNGG 5 cut(s) 129, 525, 552, 659, 716
BsmFI GGGAC 1 cut(s) 209
BsnI GGCC 1 cut(s) 11
BsoBI CYCGRG 2 cut(s) 586, 767
Bsp119I TTCGAA 1 cut(s) 868
Bsp1286I GDGCHC 3 cut(s) 95, 387, 1108
Bsp143I GATC 6 cut(s) 3, 225, 309, 346, 809, 1075
Bsp19I CCATGG 1 cut(s) 658
BspANI GGCC 1 cut(s) 11
BspCNI CTCAG 2 cut(s) 478, 1168
BspFNI CGCG 2 cut(s) 8, 1173
BspLI GGNNCC 2 cut(s) 197, 198
BspMAI CTGCAG 1 cut(s) 346
BspOI GCTAGC 1 cut(s) 452
BspPI GGATC 1 cut(s) 317
BspQI GCTCTTC 1 cut(s) 769
BspT104I TTCGAA 1 cut(s) 868
BsrBI CCGCTC 1 cut(s) 22
BsrDI GCAATG 1 cut(s) 690
BsrI ACTGG 1 cut(s) 335
BssECI CCNNGG 8 cut(s) 106, 199, 208, 264, 279, 585, 658, 1163
BssMI GATC 6 cut(s) 3, 225, 309, 346, 809, 1075
BssNI GRCGYC 1 cut(s) 991
BssT1I CCWWGG 4 cut(s) 106, 264, 658, 1163
Bst2UI CCWGG 1 cut(s) 209
Bst4CI ACNGT 3 cut(s) 389, 700, 884
Bst6I CTCTTC 7 cut(s) 409, 450, 769, 830, 864, 1125, 1229
BstACI GRCGYC 1 cut(s) 991
BstAPI GCANNNNNTGC 2 cut(s) 542, 914
BstBAI YACGTR 1 cut(s) 914
BstBI TTCGAA 1 cut(s) 868
BstC8I GCNNGC 1 cut(s) 450
BstDEI CTNAG 3 cut(s) 486, 520, 1155
BstDSI CCRYGG 1 cut(s) 658
BstEII GGTNACC 1 cut(s) 203
BstF5I GGATG 4 cut(s) 159, 191, 1183, 1270
BstFNI CGCG 2 cut(s) 8, 1173
BstH2I RGCGCY 2 cut(s) 89, 276
BstHHI GCGC 3 cut(s) 88, 275, 793
BstKTI GATC 6 cut(s) 6, 228, 312, 349, 812, 1078
BstMBI GATC 6 cut(s) 3, 225, 309, 346, 809, 1075
BstMCI CGRYCG 1 cut(s) 702
BstMWI GCNNNNNNNGC 2 cut(s) 542, 914
BstNI CCWGG 1 cut(s) 209
BstPAI GACNNNNGTC 1 cut(s) 201
BstPI GGTNACC 1 cut(s) 203
BstSCI CCNGG 4 cut(s) 192, 199, 207, 279
BstSFI CTRYAG 1 cut(s) 342
BstSLI GKGCMC 1 cut(s) 387
BstUI CGCG 2 cut(s) 8, 1173
BstV1I GCAGC 2 cut(s) 282, 328
BstV2I GAAGAC 1 cut(s) 749
BstX2I RGATCY 1 cut(s) 809
BstYI RGATCY 1 cut(s) 809
Bsu36I CCTNAGG 1 cut(s) 520
BsuRI GGCC 1 cut(s) 11
BtgI CCRYGG 1 cut(s) 658
BtgZI GCGATG 1 cut(s) 56
BtsCI GGATG 4 cut(s) 159, 191, 1183, 1270
BtsI GCAGTG 1 cut(s) 325
BtsIMutI CAGTG 1 cut(s) 325
Cac8I GCNNGC 1 cut(s) 450
CfoI GCGC 3 cut(s) 88, 275, 793
Cfr13I GGNCC 1 cut(s) 196
CseI GACGC 2 cut(s) 914, 980
Csp6I GTAC 2 cut(s) 491, 663
CviAII CATG 1 cut(s) 659
CviQI GTAC 2 cut(s) 491, 663
DdeI CTNAG 3 cut(s) 486, 520, 1155
DpnI GATC 6 cut(s) 5, 227, 311, 348, 811, 1077
DpnII GATC 6 cut(s) 3, 225, 309, 346, 809, 1075
EaeI YGGCCR 1 cut(s) 9
Eam1104I CTCTTC 7 cut(s) 409, 450, 769, 830, 864, 1125, 1229
EarI CTCTTC 7 cut(s) 409, 450, 769, 830, 864, 1125, 1229
EciI GGCGGA 2 cut(s) 40, 155
Eco130I CCWWGG 4 cut(s) 106, 264, 658, 1163
Eco32I GATATC 1 cut(s) 1040
Eco47I GGWCC 1 cut(s) 196
Eco57I CTGAAG 2 cut(s) 309, 474
Eco81I CCTNAGG 1 cut(s) 520
Eco88I CYCGRG 2 cut(s) 586, 767
Eco91I GGTNACC 1 cut(s) 203
EcoO109I RGGNCCY 1 cut(s) 196
EcoO65I GGTNACC 1 cut(s) 203
EcoRII CCWGG 1 cut(s) 207
EcoRV GATATC 1 cut(s) 1040
EcoT14I CCWWGG 4 cut(s) 106, 264, 658, 1163
ErhI CCWWGG 4 cut(s) 106, 264, 658, 1163
FaeI CATG 1 cut(s) 662
FalI AAGNNNNNCTT 2 cut(s) 809, 841
FaqI GGGAC 1 cut(s) 209
FatI CATG 1 cut(s) 658
FauI CCCGC 1 cut(s) 230
FauNDI CATATG 2 cut(s) 937, 1278
FbaI TGATCA 1 cut(s) 1075
Fnu4HI GCNGC 6 cut(s) 9, 235, 255, 271, 276, 342
FokI GGATG 4 cut(s) 166, 198, 1190, 1277
Fsp4HI GCNGC 6 cut(s) 9, 235, 255, 271, 276, 342
FspBI CTAG 2 cut(s) 107, 449
FspI TGCGCA 1 cut(s) 792
GlaI GCGC 3 cut(s) 87, 274, 792
GluI GCNGC 6 cut(s) 9, 235, 255, 271, 276, 342
GsaI CCCAGC 1 cut(s) 807
GsuI CTGGAG 2 cut(s) 79, 352
HaeII RGCGCY 2 cut(s) 89, 276
HaeIII GGCC 1 cut(s) 11
HapII CCGG 5 cut(s) 180, 193, 201, 281, 988
HgaI GACGC 2 cut(s) 914, 980
HhaI GCGC 3 cut(s) 88, 275, 793
Hin1I GRCGYC 1 cut(s) 991
Hin1II CATG 1 cut(s) 662
Hin6I GCGC 3 cut(s) 86, 273, 791
HinP1I GCGC 3 cut(s) 86, 273, 791
HincII GTYRAC 1 cut(s) 430
HindII GTYRAC 1 cut(s) 430
HindIII AAGCTT 1 cut(s) 362
HinfI GANTC 1 cut(s) 148
HpaII CCGG 5 cut(s) 180, 193, 201, 281, 988
HphI GGTGA 4 cut(s) 197, 452, 647, 1243
Hpy166II GTNNAC 3 cut(s) 385, 430, 1117
Hpy188III TCNNGA 5 cut(s) 368, 408, 767, 769, 1109
Hpy8I GTNNAC 3 cut(s) 385, 430, 1117
Hpy99I CGWCG 1 cut(s) 930
HpyAV CCTTC 1 cut(s) 546
HpyCH4III ACNGT 3 cut(s) 389, 700, 884
HpyCH4IV ACGT 2 cut(s) 913, 1113
HpyCH4V TGCA 4 cut(s) 344, 385, 536, 917
HpyF10VI GCNNNNNNNGC 2 cut(s) 542, 914
HpyF3I CTNAG 3 cut(s) 486, 520, 1155
HpySE526I ACGT 2 cut(s) 913, 1113
Hsp92I GRCGYC 1 cut(s) 991
Hsp92II CATG 1 cut(s) 662
HspAI GCGC 3 cut(s) 86, 273, 791
KflI GGGWCCC 1 cut(s) 196
Ksp22I TGATCA 1 cut(s) 1075
Kzo9I GATC 6 cut(s) 3, 225, 309, 346, 809, 1075
LguI GCTCTTC 1 cut(s) 769
LmnI GCTCC 4 cut(s) 98, 122, 168, 744
Lsp1109I GCAGC 2 cut(s) 282, 328
LweI GCATC 4 cut(s) 79, 176, 829, 1103
MaeI CTAG 2 cut(s) 107, 449
MaeII ACGT 2 cut(s) 913, 1113
MaeIII GTNAC 4 cut(s) 139, 203, 398, 1024
MalI GATC 6 cut(s) 5, 227, 311, 348, 811, 1077
MbiI CCGCTC 1 cut(s) 22
MboI GATC 6 cut(s) 3, 225, 309, 346, 809, 1075
MfeI CAATTG 1 cut(s) 666
MflI RGATCY 1 cut(s) 809
MhlI GDGCHC 3 cut(s) 95, 387, 1108
MluCI AATT 7 cut(s) 25, 609, 628, 666, 896, 949, 1249
MluI ACGCGT 1 cut(s) 1171
MlyI GAGTC 1 cut(s) 142
MmeI TCCRAC 1 cut(s) 1038
MroXI GAANNNNTTC 2 cut(s) 567, 778
MseI TTAA 4 cut(s) 216, 876, 921, 1188
MslI CAYNNNNRTG 2 cut(s) 390, 1229
MspA1I CMGCKG 1 cut(s) 234
MspI CCGG 5 cut(s) 180, 193, 201, 281, 988
MspR9I CCNGG 4 cut(s) 194, 201, 209, 281
MunI CAATTG 1 cut(s) 666
MvaI CCWGG 1 cut(s) 209
MvnI CGCG 2 cut(s) 8, 1173
MwoI GCNNNNNNNGC 2 cut(s) 542, 914
NciI CCSGG 3 cut(s) 194, 201, 281
NcoI CCATGG 1 cut(s) 658
NdeI CATATG 2 cut(s) 937, 1278
NdeII GATC 6 cut(s) 3, 225, 309, 346, 809, 1075
NheI GCTAGC 1 cut(s) 448
NlaIII CATG 1 cut(s) 662
NlaIV GGNNCC 2 cut(s) 197, 198
NmuCI GTSAC 2 cut(s) 139, 203
NsbI TGCGCA 1 cut(s) 792
NspV TTCGAA 1 cut(s) 868
OliI CACNNNNGTG 1 cut(s) 390
PaeR7I CTCGAG 1 cut(s) 767
PciSI GCTCTTC 1 cut(s) 769
PdmI GAANNNNTTC 2 cut(s) 567, 778
PflMI CCANNNNNTGG 1 cut(s) 716
PfoI TCCNGGA 1 cut(s) 192
PkrI GCNGC 6 cut(s) 10, 236, 256, 272, 277, 343
PleI GAGTC 1 cut(s) 142
PpsI GAGTC 1 cut(s) 142
Ppu21I YACGTR 1 cut(s) 914
PpuMI RGGWCCY 1 cut(s) 196
PshAI GACNNNNGTC 1 cut(s) 201
PsiI TTATAA 1 cut(s) 695
Psp5II RGGWCCY 1 cut(s) 196
Psp6I CCWGG 1 cut(s) 207
PspEI GGTNACC 1 cut(s) 203
PspFI CCCAGC 1 cut(s) 803
PspGI CCWGG 1 cut(s) 207
PspN4I GGNNCC 2 cut(s) 197, 198
PspPI GGNCC 1 cut(s) 196
PspPPI RGGWCCY 1 cut(s) 196
PstI CTGCAG 1 cut(s) 346
PsuI RGATCY 1 cut(s) 809
RsaI GTAC 2 cut(s) 492, 664
RsaNI GTAC 2 cut(s) 491, 663
RseI CAYNNNNRTG 2 cut(s) 390, 1229
SapI GCTCTTC 1 cut(s) 769
SaqAI TTAA 4 cut(s) 216, 876, 921, 1188
SatI GCNGC 6 cut(s) 9, 235, 255, 271, 276, 342
Sau3AI GATC 6 cut(s) 3, 225, 309, 346, 809, 1075
Sau96I GGNCC 1 cut(s) 196
SchI GAGTC 1 cut(s) 142
ScrFI CCNGG 4 cut(s) 194, 201, 209, 281
SduI GDGCHC 3 cut(s) 95, 387, 1108
SfaNI GCATC 4 cut(s) 79, 176, 829, 1103
SfcI CTRYAG 1 cut(s) 342
Sfr274I CTCGAG 1 cut(s) 767
SfuI TTCGAA 1 cut(s) 868
SinI GGWCC 1 cut(s) 196
SlaI CTCGAG 1 cut(s) 767
SmiMI CAYNNNNRTG 2 cut(s) 390, 1229
SmlI CTYRAG 2 cut(s) 767, 1107
SmoI CTYRAG 2 cut(s) 767, 1107
Sse9I AATT 7 cut(s) 25, 609, 628, 666, 896, 949, 1249
SspMI CTAG 2 cut(s) 107, 449
StyD4I CCNGG 4 cut(s) 192, 199, 207, 279
StyI CCWWGG 4 cut(s) 106, 264, 658, 1163
TaaI ACNGT 3 cut(s) 389, 700, 884
TaiI ACGT 2 cut(s) 916, 1116
TaqI TCGA 4 cut(s) 81, 228, 768, 868
TasI AATT 7 cut(s) 25, 609, 628, 666, 896, 949, 1249
TauI GCSGC 4 cut(s) 11, 237, 257, 278
Tru1I TTAA 4 cut(s) 216, 876, 921, 1188
Tru9I TTAA 4 cut(s) 216, 876, 921, 1188
TscAI CASTG 1 cut(s) 325
TseFI GTSAC 2 cut(s) 139, 203
TseI GCWGC 2 cut(s) 270, 341
Tsp45I GTSAC 2 cut(s) 139, 203
TspDTI ATGAA 5 cut(s) 576, 701, 889, 1014, 1073
TspGWI ACGGA 2 cut(s) 35, 508
TspRI CASTG 1 cut(s) 325
Van91I CCANNNNNTGG 1 cut(s) 716
VneI GTGCAC 1 cut(s) 383
VpaK11BI GGWCC 1 cut(s) 196
XapI RAATTY 1 cut(s) 896
XcmI CCANNNNNNNNNTGG 1 cut(s) 656
XhoI CTCGAG 1 cut(s) 767
XmaJI CCTAGG 1 cut(s) 106
XmnI GAANNNNTTC 2 cut(s) 567, 778
XspI CTAG 2 cut(s) 107, 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.