pycom16g24390

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0012280
Physical Location & Seq
Reverse (-)
25816339 .. 25822472
6134 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1467 bp
ATGGCGTCCGAAATCTCAAACGACAAGACCGCTGTTTTCAGAAAACTGAAAGCAAAGTCAGAAAACAAGATGTGTTTCGATTGTAATGCAAAGAACCCGACTTGGGCATCGGTTACGTATGGGATCTTTCTCTGCATCGATTGCTCGGCCGTTCATCGCAGTCTCGGCGTTCATATCAGCTTTGTTAGGTCTACAAATTTAGACTCATGGACTCCTGAACAACTGAGAACAATGAGCTTCGGGGGAAACAACCGTGCACAAGTGTTCTTTAAGCAGCATGGATGGACGGATGGAGGCAAAATTGAGGCTAAGTATACATCAAGAGCTGCCGAGTTGTATAGGCAAATACTTTCTAAAGAAGTAGTCAAAAGTATGGCAGAAGAGGCAGGTCTTCCACCATCACCTGTTGCATCTCAGTCAGGACAAGCATCTAATGGGATTCTTGATGTTAAGACCCAGGAAGCTCCAGAAGAGAACTCTATAGCAAGGAAAGAAACGCCTGAAATCACTGCTTCCCAGAAAGCCCCCCATACATATGTTACCAGTACTGTTAAGAAGCCTCTTGGTGGTGCTAAGAAAATTGGGAAGACTGGGGGGCTTGGTGCTCGCAAACTTACTACAAAGCCTAATGAAAGCCTGTACGATCAGAAACCTGAAGATCCAGTTGTCCCAGTGCCAGTGCCTTCCTCAACTAACAGCACTCCAACAGCTGGATCATCCTTTGCATCTCGATTTGAGTATGTAGAAATTGTCCAAAATACTGAGACAAATCCCACTGGCGCTCATGTAATTAGCCATATTGCTCCACCGAAGTCATCAAATTTCTTTGCAGATTTTGGAATGGACAGTAACTTTCCCAGGAAAACTAATTCAAATACCTCAAAAGTTCAAATTCAGGAGTCTGATGAAGCAAGAAAGAAATTCACAAATGCAAAATCTATCTCTTCTGCACAATTTTTTGGTGACCAAAGCAGATCTGCTGATGTTGATGCTCAAACCTCCTTGCAGAAGTTCTCGGGTTCTGCAGCCATCTCCAGCGCTGATCTATTTGGTGACAGGGGAGATAATTCAGCTATTGATCTTACTGCAAGCGACCTAATCAACCGACTGTCGTTTCAGGCACAACAGGATCTCTCTTCCTTAACGAATATCGCTGGAGAGACCGGGAAGAAGCTTAGTTCCTTGGCGTCCAATTTAATGACGGATCTTCAGGACAGAATCCTACTTGACGAAAATTCTCAAGGCCTAAGAGTGGCGGGTTGGCGGGTGATTTGTTTCGTAGAACAGGAAACTATTATTGTTCTGAGATTGTGGATTATATGTGCTCTTGTGAAACACCGTAACATTACTGCTCTGTGTCAAGGGAGCAACGCGAGAATACTGTATTCTCTTAAAACCGTGGATTTGTCCCTGCCCAATTTTAATTCAATCTCGAATACGGGTTCCTTGCAAGGAACGTGTTGTTGA

Protein Analysis

489

Amino Acids

52.93

Weight (kDa)

8.71

Isoelectric Point (pI)

38.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArfGap PF01412 12 - 115 9.1e-36 Putative GTPase activating protein for Arf
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012280)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17890 AT4G17890
fragaria_vesca FvH4_5g22130
malus_domestica MD00G1122000.v1.1 MD00G1183400.v1.1 MD06G1034600.v1.1
prunus_persica Prupe.5G035300_v2.0.a1
pyrus_communis pycom06g02860 pycom16g24390
rosa_chinensis RchiOBHm_Chr7g0209771
rosa_laevigata RLG00000003094
rosa_multiflora Rmu_sc0001818.1_g000012
rosa_roxburghii Rroxscaffold_3G00248980
rosa_rugosa Rorug07G0116100
rosa_samantha Rh7AG249900 Rh7BG243800 Rh7CG265900 Rh7DG255700
rosa_wichuraiana Rw7G021170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 377
AccB7I CCANNNNNTGG 1 cut(s) 710
AccI GTMKAC 2 cut(s) 191, 314
AccII CGCG 1 cut(s) 1373
AciI CCGC 3 cut(s) 30, 1256, 1264
AclWI GGATC 5 cut(s) 131, 653, 721, 1137, 1212
AcoI YGGCCR 1 cut(s) 147
AcsI RAATTY 5 cut(s) 196, 820, 891, 920, 1234
AcuI CTGAAG 2 cut(s) 675, 1193
AcyI GRCGYC 2 cut(s) 5, 1187
AfaI GTAC 2 cut(s) 547, 641
AfeI AGCGCT 1 cut(s) 1039
AfiI CCNNNNNNNGG 4 cut(s) 103, 566, 710, 1252
AflIII ACRYGT 1 cut(s) 1457
AgsI TTSAA 3 cut(s) 873, 890, 1428
AjnI CCWGG 2 cut(s) 456, 857
AluBI AGCT 7 cut(s) 180, 237, 326, 464, 710, 1073, 1174
AluI AGCT 7 cut(s) 180, 237, 326, 464, 710, 1073, 1174
Alw21I GWGCWC 3 cut(s) 259, 607, 1327
Alw26I GTCTC 3 cut(s) 167, 758, 1154
Alw44I GTGCAC 1 cut(s) 255
AlwI GGATC 5 cut(s) 131, 653, 721, 1137, 1212
Ama87I CYCGRG 1 cut(s) 1015
Aor51HI AGCGCT 1 cut(s) 1039
AoxI GGCC 2 cut(s) 147, 1243
ApaLI GTGCAC 1 cut(s) 255
ApeKI GCWGC 3 cut(s) 274, 326, 1025
ApoI RAATTY 5 cut(s) 196, 820, 891, 920, 1234
AspLEI GCGC 2 cut(s) 782, 1040
AsuC2I CCSGG 1 cut(s) 1165
AsuHPI GGTGA 4 cut(s) 393, 974, 1064, 1279
AvaI CYCGRG 1 cut(s) 1015
BaeGI GKGCMC 1 cut(s) 259
BbsI GAAGAC 2 cut(s) 383, 593
Bbv12I GWGCWC 3 cut(s) 259, 607, 1327
BbvI GCAGC 3 cut(s) 286, 313, 1037
BccI CCATC 4 cut(s) 276, 284, 406, 1037
BceAI ACGGC 1 cut(s) 134
BcgI CGANNNNNNTGC 2 cut(s) 68, 102
BciT130I CCWGG 2 cut(s) 458, 859
BcnI CCSGG 1 cut(s) 1165
BcoDI GTCTC 3 cut(s) 167, 758, 1154
BfmI CTRYAG 2 cut(s) 480, 1023
BfoI RGCGCY 2 cut(s) 783, 1041
BfuAI ACCTGC 1 cut(s) 377
BglII AGATCT 1 cut(s) 974
BisI GCNGC 3 cut(s) 275, 327, 1026
BlsI GCNGC 3 cut(s) 276, 328, 1027
BmcAI AGTACT 1 cut(s) 547
Bme1390I CCNGG 3 cut(s) 458, 859, 1165
BmeT110I CYCGRG 1 cut(s) 1015
BmiI GGNNCC 1 cut(s) 1444
BmrFI CCNGG 3 cut(s) 458, 859, 1165
BmrI ACTGGG 2 cut(s) 600, 665
BmsI GCATC 6 cut(s) 116, 144, 419, 437, 734, 979
BmuI ACTGGG 2 cut(s) 600, 665
BpiI GAAGAC 2 cut(s) 383, 593
BpmI CTGGAG 3 cut(s) 450, 1018, 1176
BpuEI CTTGAG 1 cut(s) 1224
BpuMI CCSGG 1 cut(s) 1165
Bsa29I ATCGAT 1 cut(s) 138
BsaAI YACGTR 1 cut(s) 117
BsaHI GRCGYC 2 cut(s) 5, 1187
BsaI GGTCTC 1 cut(s) 1154
BsaJI CCNNGG 4 cut(s) 456, 857, 1182, 1398
Bsc4I CCNNNNNNNGG 4 cut(s) 103, 566, 710, 1252
Bse1I ACTGG 6 cut(s) 543, 595, 662, 671, 677, 781
BseBI CCWGG 2 cut(s) 458, 859
BseCI ATCGAT 1 cut(s) 138
BseDI CCNNGG 4 cut(s) 456, 857, 1182, 1398
BseGI GGATG 3 cut(s) 287, 295, 716
BseLI CCNNNNNNNGG 4 cut(s) 103, 566, 710, 1252
BseMII CTCAG 4 cut(s) 215, 428, 753, 1295
BseNI ACTGG 6 cut(s) 543, 595, 662, 671, 677, 781
BseSI GKGCMC 1 cut(s) 259
BseX3I CGGCCG 1 cut(s) 147
BseXI GCAGC 3 cut(s) 286, 313, 1037
BsgI GTGCAG 1 cut(s) 933
Bsh1236I CGCG 1 cut(s) 1373
Bsh1285I CGRYCG 1 cut(s) 150
BshFI GGCC 2 cut(s) 149, 1245
BshVI ATCGAT 1 cut(s) 138
BsiEI CGRYCG 1 cut(s) 150
BsiHKAI GWGCWC 3 cut(s) 259, 607, 1327
BsiHKCI CYCGRG 1 cut(s) 1015
BsiSI CCGG 1 cut(s) 1164
BslFI GGGAC 2 cut(s) 653, 1393
BslI CCNNNNNNNGG 4 cut(s) 103, 566, 710, 1252
BsmAI GTCTC 3 cut(s) 167, 758, 1154
BsmFI GGGAC 2 cut(s) 653, 1393
BsnI GGCC 2 cut(s) 149, 1245
Bso31I GGTCTC 1 cut(s) 1154
BsoBI CYCGRG 1 cut(s) 1015
Bsp1286I GDGCHC 3 cut(s) 259, 607, 1327
Bsp143I GATC 9 cut(s) 123, 643, 658, 713, 974, 1042, 1078, 1129, 1204
BspACI CCGC 3 cut(s) 30, 1256, 1264
BspANI GGCC 2 cut(s) 149, 1245
BspCNI CTCAG 4 cut(s) 216, 427, 754, 1296
BspDI ATCGAT 1 cut(s) 138
BspFNI CGCG 1 cut(s) 1373
BspLI GGNNCC 1 cut(s) 1444
BspMAI CTGCAG 1 cut(s) 1027
BspMI ACCTGC 1 cut(s) 377
BspPI GGATC 5 cut(s) 131, 653, 721, 1137, 1212
BspTNI GGTCTC 1 cut(s) 1154
BsrI ACTGG 6 cut(s) 543, 595, 662, 671, 677, 781
BssECI CCNNGG 4 cut(s) 456, 857, 1182, 1398
BssMI GATC 9 cut(s) 123, 643, 658, 713, 974, 1042, 1078, 1129, 1204
BssNAI GTATAC 1 cut(s) 315
BssNI GRCGYC 2 cut(s) 5, 1187
BssT1I CCWWGG 1 cut(s) 1182
Bst1107I GTATAC 1 cut(s) 315
Bst2UI CCWGG 2 cut(s) 458, 859
Bst4CI ACNGT 7 cut(s) 254, 550, 848, 1110, 1340, 1383, 1399
Bst6I CTCTTC 4 cut(s) 375, 465, 949, 1141
BstACI GRCGYC 2 cut(s) 5, 1187
BstAPI GCANNNNNTGC 1 cut(s) 141
BstBAI YACGTR 1 cut(s) 117
BstC8I GCNNGC 2 cut(s) 607, 1090
BstDEI CTNAG 8 cut(s) 224, 309, 414, 573, 762, 1175, 1247, 1304
BstDSI CCRYGG 1 cut(s) 1398
BstEII GGTNACC 1 cut(s) 962
BstF5I GGATG 3 cut(s) 287, 295, 716
BstFNI CGCG 1 cut(s) 1373
BstH2I RGCGCY 2 cut(s) 783, 1041
BstHHI GCGC 2 cut(s) 782, 1040
BstKTI GATC 9 cut(s) 126, 646, 661, 716, 977, 1045, 1081, 1132, 1207
BstMAI GTCTC 3 cut(s) 167, 758, 1154
BstMBI GATC 9 cut(s) 123, 643, 658, 713, 974, 1042, 1078, 1129, 1204
BstMCI CGRYCG 1 cut(s) 150
BstMWI GCNNNNNNNGC 3 cut(s) 141, 165, 383
BstNI CCWGG 2 cut(s) 458, 859
BstPI GGTNACC 1 cut(s) 962
BstSCI CCNGG 3 cut(s) 456, 857, 1163
BstSFI CTRYAG 2 cut(s) 480, 1023
BstSLI GKGCMC 1 cut(s) 259
BstSNI TACGTA 1 cut(s) 117
BstUI CGCG 1 cut(s) 1373
BstV1I GCAGC 3 cut(s) 286, 313, 1037
BstV2I GAAGAC 2 cut(s) 383, 593
BstX2I RGATCY 5 cut(s) 123, 658, 974, 1129, 1204
BstYI RGATCY 5 cut(s) 123, 658, 974, 1129, 1204
BstZ17I GTATAC 1 cut(s) 315
BstZI CGGCCG 1 cut(s) 147
Bsu15I ATCGAT 1 cut(s) 138
BsuRI GGCC 2 cut(s) 149, 1245
BsuTUI ATCGAT 1 cut(s) 138
BtgI CCRYGG 1 cut(s) 1398
BtgZI GCGATG 1 cut(s) 140
BtsCI GGATG 3 cut(s) 287, 295, 716
BtsI GCAGTG 1 cut(s) 507
BtsIMutI CAGTG 4 cut(s) 507, 678, 684, 774
BveI ACCTGC 1 cut(s) 377
Cac8I GCNNGC 2 cut(s) 607, 1090
CfoI GCGC 2 cut(s) 782, 1040
ClaI ATCGAT 1 cut(s) 138
CseI GACGC 1 cut(s) 1176
Csp6I GTAC 2 cut(s) 546, 640
CviAII CATG 3 cut(s) 207, 278, 785
CviQI GTAC 2 cut(s) 546, 640
DdeI CTNAG 8 cut(s) 224, 309, 414, 573, 762, 1175, 1247, 1304
DpnI GATC 9 cut(s) 125, 645, 660, 715, 976, 1044, 1080, 1131, 1206
DpnII GATC 9 cut(s) 123, 643, 658, 713, 974, 1042, 1078, 1129, 1204
EaeI YGGCCR 1 cut(s) 147
EagI CGGCCG 1 cut(s) 147
Eam1104I CTCTTC 4 cut(s) 375, 465, 949, 1141
EarI CTCTTC 4 cut(s) 375, 465, 949, 1141
EclXI CGGCCG 1 cut(s) 147
Eco105I TACGTA 1 cut(s) 117
Eco130I CCWWGG 1 cut(s) 1182
Eco147I AGGCCT 1 cut(s) 1245
Eco31I GGTCTC 1 cut(s) 1154
Eco47III AGCGCT 1 cut(s) 1039
Eco52I CGGCCG 1 cut(s) 147
Eco57I CTGAAG 2 cut(s) 675, 1193
Eco88I CYCGRG 1 cut(s) 1015
Eco91I GGTNACC 1 cut(s) 962
EcoO65I GGTNACC 1 cut(s) 962
EcoRII CCWGG 2 cut(s) 456, 857
EcoT14I CCWWGG 1 cut(s) 1182
ErhI CCWWGG 1 cut(s) 1182
FaeI CATG 3 cut(s) 210, 281, 788
FaqI GGGAC 2 cut(s) 653, 1393
FatI CATG 3 cut(s) 206, 277, 784
FauI CCCGC 2 cut(s) 1249, 1257
FauNDI CATATG 1 cut(s) 535
FblI GTMKAC 2 cut(s) 191, 314
Fnu4HI GCNGC 3 cut(s) 275, 327, 1026
FokI GGATG 3 cut(s) 294, 302, 703
Fsp4HI GCNGC 3 cut(s) 275, 327, 1026
GlaI GCGC 2 cut(s) 781, 1039
GluI GCNGC 3 cut(s) 275, 327, 1026
GsuI CTGGAG 3 cut(s) 450, 1018, 1176
HaeII RGCGCY 2 cut(s) 783, 1041
HaeIII GGCC 2 cut(s) 149, 1245
HapII CCGG 1 cut(s) 1164
HgaI GACGC 1 cut(s) 1176
HhaI GCGC 2 cut(s) 782, 1040
Hin1I GRCGYC 2 cut(s) 5, 1187
Hin1II CATG 3 cut(s) 210, 281, 788
Hin6I GCGC 2 cut(s) 780, 1038
HinP1I GCGC 2 cut(s) 780, 1038
HindIII AAGCTT 1 cut(s) 1172
HinfI GANTC 5 cut(s) 203, 211, 439, 899, 1218
HpaII CCGG 1 cut(s) 1164
HphI GGTGA 4 cut(s) 393, 974, 1064, 1279
Hpy166II GTNNAC 3 cut(s) 192, 257, 315
Hpy188I TCNGA 6 cut(s) 10, 41, 61, 648, 904, 1305
Hpy188III TCNNGA 9 cut(s) 215, 321, 420, 443, 467, 729, 896, 1211, 1432
Hpy8I GTNNAC 3 cut(s) 192, 257, 315
HpyAV CCTTC 1 cut(s) 693
HpyCH4III ACNGT 7 cut(s) 254, 550, 848, 1110, 1340, 1383, 1399
HpyCH4IV ACGT 2 cut(s) 116, 1457
HpyF10VI GCNNNNNNNGC 3 cut(s) 141, 165, 383
HpyF3I CTNAG 8 cut(s) 224, 309, 414, 573, 762, 1175, 1247, 1304
HpySE526I ACGT 2 cut(s) 116, 1457
Hsp92I GRCGYC 2 cut(s) 5, 1187
Hsp92II CATG 3 cut(s) 210, 281, 788
HspAI GCGC 2 cut(s) 780, 1038
Kzo9I GATC 9 cut(s) 123, 643, 658, 713, 974, 1042, 1078, 1129, 1204
LmnI GCTCC 3 cut(s) 469, 808, 1365
Lsp1109I GCAGC 3 cut(s) 286, 313, 1037
LweI GCATC 6 cut(s) 116, 144, 419, 437, 734, 979
MaeII ACGT 2 cut(s) 116, 1457
MaeIII GTNAC 6 cut(s) 112, 538, 848, 962, 1052, 1340
MalI GATC 9 cut(s) 125, 645, 660, 715, 976, 1044, 1080, 1131, 1206
MboI GATC 9 cut(s) 123, 643, 658, 713, 974, 1042, 1078, 1129, 1204
MboII GAAGA 9 cut(s) 383, 392, 482, 598, 668, 936, 1128, 1180, 1199
MflI RGATCY 5 cut(s) 123, 658, 974, 1129, 1204
MhlI GDGCHC 3 cut(s) 259, 607, 1327
MlyI GAGTC 3 cut(s) 197, 205, 908
MmeI TCCRAC 1 cut(s) 728
MnlI CCTC 7 cut(s) 287, 298, 376, 570, 697, 889, 1009
MseI TTAA 7 cut(s) 270, 450, 552, 1142, 1196, 1392, 1422
MslI CAYNNNNRTG 1 cut(s) 534
MspA1I CMGCKG 2 cut(s) 32, 710
MspI CCGG 1 cut(s) 1164
MspR9I CCNGG 3 cut(s) 458, 859, 1165
MvaI CCWGG 2 cut(s) 458, 859
MvnI CGCG 1 cut(s) 1373
MwoI GCNNNNNNNGC 3 cut(s) 141, 165, 383
NciI CCSGG 1 cut(s) 1165
NdeI CATATG 1 cut(s) 535
NdeII GATC 9 cut(s) 123, 643, 658, 713, 974, 1042, 1078, 1129, 1204
NlaIII CATG 3 cut(s) 210, 281, 788
NlaIV GGNNCC 1 cut(s) 1444
NmeAIII GCCGAG 3 cut(s) 125, 144, 355
NmuCI GTSAC 2 cut(s) 962, 1052
PceI AGGCCT 1 cut(s) 1245
PfeI GAWTC 2 cut(s) 439, 1218
PflMI CCANNNNNTGG 1 cut(s) 710
PkrI GCNGC 3 cut(s) 276, 328, 1027
PleI GAGTC 3 cut(s) 197, 205, 907
PpsI GAGTC 3 cut(s) 197, 205, 907
Ppu21I YACGTR 1 cut(s) 117
Psp6I CCWGG 2 cut(s) 456, 857
PspEI GGTNACC 1 cut(s) 962
PspGI CCWGG 2 cut(s) 456, 857
PspN4I GGNNCC 1 cut(s) 1444
PstI CTGCAG 1 cut(s) 1027
PsuI RGATCY 5 cut(s) 123, 658, 974, 1129, 1204
PvuII CAGCTG 1 cut(s) 710
RsaI GTAC 2 cut(s) 547, 641
RsaNI GTAC 2 cut(s) 546, 640
RseI CAYNNNNRTG 1 cut(s) 534
SaqAI TTAA 7 cut(s) 270, 450, 552, 1142, 1196, 1392, 1422
SatI GCNGC 3 cut(s) 275, 327, 1026
Sau3AI GATC 9 cut(s) 123, 643, 658, 713, 974, 1042, 1078, 1129, 1204
ScaI AGTACT 1 cut(s) 547
SchI GAGTC 3 cut(s) 197, 205, 908
ScrFI CCNGG 3 cut(s) 458, 859, 1165
SduI GDGCHC 3 cut(s) 259, 607, 1327
SfaNI GCATC 6 cut(s) 116, 144, 419, 437, 734, 979
SfcI CTRYAG 2 cut(s) 480, 1023
SmiMI CAYNNNNRTG 1 cut(s) 534
SmlI CTYRAG 1 cut(s) 1239
SmoI CTYRAG 1 cut(s) 1239
SnaBI TACGTA 1 cut(s) 117
SseBI AGGCCT 1 cut(s) 1245
SsiI CCGC 3 cut(s) 30, 1256, 1264
StuI AGGCCT 1 cut(s) 1245
StyD4I CCNGG 3 cut(s) 456, 857, 1163
StyI CCWWGG 1 cut(s) 1182
TaaI ACNGT 7 cut(s) 254, 550, 848, 1110, 1340, 1383, 1399
TaiI ACGT 2 cut(s) 119, 1460
TaqI TCGA 4 cut(s) 78, 138, 730, 1433
TatI WGTACW 1 cut(s) 545
TfiI GAWTC 2 cut(s) 439, 1218
Tru1I TTAA 7 cut(s) 270, 450, 552, 1142, 1196, 1392, 1422
Tru9I TTAA 7 cut(s) 270, 450, 552, 1142, 1196, 1392, 1422
TscAI CASTG 4 cut(s) 514, 678, 684, 781
TseFI GTSAC 2 cut(s) 962, 1052
TseI GCWGC 3 cut(s) 274, 326, 1025
Tsp45I GTSAC 2 cut(s) 962, 1052
TspDTI ATGAA 4 cut(s) 143, 161, 645, 921
TspGWI ACGGA 2 cut(s) 302, 1217
TspRI CASTG 4 cut(s) 514, 678, 684, 781
Van91I CCANNNNNTGG 1 cut(s) 710
VneI GTGCAC 1 cut(s) 255
XapI RAATTY 5 cut(s) 196, 820, 891, 920, 1234
XmiI GTMKAC 2 cut(s) 191, 314
ZrmI AGTACT 1 cut(s) 547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.