Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0012456

Physical Location & Seq

Genomic Coordinates Forward (+)

26250841 .. 26253191

Gene Structure 2351 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom16g24660.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2229 bp

ATGGCTACCAGTGCCGTCCACTGGACTCTGAATTCCTCGGATACAAAGCGACCCATCTTCTTCTCTTCACCTTTTCGACAAATCCCAACCAAAAAATTCAACATTTCTTGCCGCTCCGCCAAGTCTCCTCCCAAATCCCCGCCGGATTTAGCCGAACCCACCACCAACAAGAAGAAGAACCAAAACCCATCTTTATCTTTGTCCGAGCAGCTCCAGCCTCTGACCACAACCACTCTCTCCGACCCTCCTCCGCCCCAAGACCAATCCCAGTTGATATCCAAGCCCAAATCCATCTGGGTCAACCCGGCCAAGCCGAAACGCTCCGTGCTCTCTCTGCAGCGGCAGAAGCGGTCTCTCGACTCTTACAATCCTCAGGTGAGAGATCTGAGGGGGTTTGCCCACAAGCTCAACGACTGTGATGCCACCAAAGTTGCCTTCTTGGCTGCCCTTGAAGAAATCCCCCACCCGCCCACACGAGAAAATGCGCTTTTGATTCTGAATAGCTTGAAGCCATGGCAGAAAACCCACATGTTCTTCAATTGGGTGAAGTCCCAGAATTTGTTTCCAATGGAAACCATATTCTACAATGTCACTATGAAGTCTCTGAGGTTCGGGAGGCAGTTTCAGCTCATCGAAGAGCTCGCGGAGGAGATGATAAGGGACGAGATTGAGCTCGATAACATTACGTATTCTACTATAATCACTTGTGCCAAGAGGTCCAAGCTTTTCGACAAGGCGGTCGAGTGGTTTGAGAGGATGTATAAGACCGGTTTGATGCCGGATGAGGTGACTTACTCTGCCATTTTAGATGTTTATGCTAAATTGGGCAAGGTTGAGGAGGTTCTTAGTTTGTATGAGAGGGGAAGAGCTAGTGGGTGGAAACCGGACCCGATTGCATTTTCGGTTTTGGGTAAGATGTTTGGGGAGGCTGGGGATTATGATGGTATTAGGTATGTGTTGCAAGAAATGGCTGCTCTTGGTGTAGAGCCTAATTTGGTTGTGTACAATACTTTGTTAGAGGCAATGGGGAAGGCCGGAAAGCCTGGTTTGGCAAGGAGCTTATTCAAAGAAATGGTGGAATCAGGGCTAACCCCGAATGAGAAAACATTGACGGCGCTTGTAAAGATCTATGGCAAGGCAAGGTGGGCTCGAGATGCTTTGGAATTGTGGGAGCGAATGAGGTCGAATAAGTGGCCTATGGACTTCATTTTGTATAACACATTGTTGAATATGTGTGCGGACCTTGGTTTGGAGGAGGAAGCTAAGACGCTTTTTGAGGATATGAAGCAGTCGGAGCATTGTAGGCCAGACAGTTGGAGTTACACTGCAATGCTGAACATCTATGGGAGCGGAGGGAATGTTGATGAGGCAATGAAATTGTTTGAAGAAATGTCCGAGTTGGGTATCGAGCTCAATGTCATGGGGTGCACTTGTTTGATTCAGGGCTTGGGGAAAGCTAGAAGATTCGGTGATATGGTCCGAGTTTTTGACGTTGCAGTTGAAAAAGGGGTTAAACCAGATGATAGGCTTTGTGGGTGCTTACTCTCTGTTGTATCGTTGTGTGAAGAAACTGAGGATGAGGATAGGGTGCTTTCTTGTTTGCAGCAGGCAAATCCGAAGTTGGTGACTCTTGTCGAAGTGCTGCAAGATAAGAAAATCGGGTTTGAGACTATTAAAGATGAGTTCAGGAACGCTATTGGTAGTACCGCGGTTGAATCCCGGAGACCCTTCTGTAATTGCTTGATTGATATATGCCGAAACAAAAACAACCATGAGAGAGCTCATGAGCTGCTCTATTTGGGAACCCTATACGGGTTGTATCCGGGTCTGCACACCAAGACTGCAAGGGAGTGGTGTTTGGACGTTAGGTCACTATCCATAGGTGCAGCTCACACTGCACTTGAAGAGTGGATGGGAACGCTCTACAAAATAGTACAGCGGGAAGAGGCGTTGCCGGAGATGTTCTCCGCTCAAACTGGTAGCGGAAGTCACAAATTCTCGCAAGGGCTGTCGCATTCCTTTGGTTCTCATGTGAAGAAATTGGCTGCGCCGTTTAGACAGAGTCAAATGGGAAATGGATTATCTCATTTGGATCGTTGTATGTTTCACTTTCAGAATAACTTTTTGTACCAGAGTAGTTATACAGTTTTCAGGCAACCTAAACGTTTAGGGAGTTGTTTGGTATCCTACTTGTTTTTCGTCCAATTTTTCGTTTTTGAAACTAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009536 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

743

Amino Acids

84.32

Weight (kDa)

8.0

Isoelectric Point (pI)

47.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_3	PF13812	214 - 273	3e-09	Pentatricopeptide repeat domain
PPR_2	PF13041	232 - 274	5.3e-07	PPR repeat family
PPR_long	PF17177	279 - 378	4.7e-07	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	319 - 379	1.7e-10	Pentatricopeptide repeat domain
PPR_1	PF12854	327 - 359	9.6e-06	PPR repeat
PPR_2	PF13041	330 - 375	3e-11	PPR repeat family
PPR	PF01535	333 - 363	1.8e-06	PPR repeat
PPR_long	PF17177	383 - 538	2.6e-07	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	403 - 446	1.3e-07	PPR repeat family
PPR_1	PF12854	433 - 465	1.6e-07	PPR repeat
PPR_2	PF13041	436 - 473	3.7e-07	PPR repeat family
PPR	PF01535	440 - 469	6e-08	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Orthologous Genes (Group: OG0012456)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G46580
fragaria_vesca	FvH4_5g21640
malus_domestica	MD06G1031400.v1.1 MD16G1277300.v1.1
prunus_persica	Prupe.5G040700_v2.0.a1
pyrus_communis	pycom06g02620 pycom16g24660
rosa_chinensis	RchiOBHm_Chr7g0209101
rosa_laevigata	RLG00000003167
rosa_multiflora	Rmu_co8470613.1_g000001 Rmu_sc0000821.1_g000010 Rmu_sc0009521.1_g000001
rosa_roxburghii	Rroxscaffold_3G00249750
rosa_rugosa	Rorug07G0109100
rosa_samantha	Rh7AG243500 Rh7BG238600 Rh7CG258700 Rh7DG250500
rosa_wichuraiana	Rw7G020640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	737
AccBSI	CCGCTC	3 cut(s)	114, 1350, 1970
AccII	CGCG	2 cut(s)	644, 1709
AclI	AACGTT	1 cut(s)	2164
AclWI	GGATC	1 cut(s)	2100
AcoI	YGGCCR	1 cut(s)	306
AcsI	RAATTY	4 cut(s)	31, 95, 556, 1994
AfaI	GTAC	4 cut(s)	1004, 1705, 1935, 2129
AfiI	CCNNNNNNNGG	3 cut(s)	21, 1249, 1812
AflIII	ACRYGT	1 cut(s)	528
AgeI	ACCGGT	1 cut(s)	767
AhdI	GACNNNNNGTC	1 cut(s)	1867
AjnI	CCWGG	1 cut(s)	1042
AjuI	GAANNNNNNNTTGG	2 cut(s)	419, 451
Alw21I	GWGCWC	6 cut(s)	330, 642, 675, 1413, 1430, 1783
Alw26I	GTCTC	5 cut(s)	129, 357, 606, 1661, 1717
Alw44I	GTGCAC	1 cut(s)	1426
AlwI	GGATC	1 cut(s)	2100
AlwNI	CAGNNNCTG	1 cut(s)	220
Ama87I	CYCGRG	1 cut(s)	1149
AoxI	GGCC	4 cut(s)	306, 1032, 1193, 1304
ApaLI	GTGCAC	1 cut(s)	1426
ApeKI	GCWGC	9 cut(s)	208, 337, 443, 971, 1603, 1642, 1789, 1886, 2045
ApoI	RAATTY	4 cut(s)	31, 95, 556, 1994
AsiGI	ACCGGT	1 cut(s)	767
AspLEI	GCGC	3 cut(s)	487, 1117, 2050
AspS9I	GGNCC	4 cut(s)	717, 886, 1240, 1477
AsuC2I	CCSGG	3 cut(s)	305, 1720, 1824
AsuHPI	GGTGA	6 cut(s)	60, 388, 556, 799, 1481, 1636
AvaI	CYCGRG	1 cut(s)	1149
AvaII	GGWCC	4 cut(s)	717, 886, 1240, 1477
AxyI	CCTNAGG	1 cut(s)	372
BaeGI	GKGCMC	1 cut(s)	1430
BanII	GRGCYC	5 cut(s)	642, 675, 1150, 1413, 1783
BauI	CACGAG	1 cut(s)	474
Bbv12I	GWGCWC	6 cut(s)	330, 642, 675, 1413, 1430, 1783
BbvI	GCAGC	9 cut(s)	220, 349, 430, 958, 1615, 1629, 1776, 1898, 2032
BccI	CCATC	5 cut(s)	62, 196, 299, 935, 1906
BceAI	ACGGC	2 cut(s)	1128, 2035
BcgI	CGANNNNNNTGC	2 cut(s)	401, 435
BciT130I	CCWGG	1 cut(s)	1044
BciVI	GTATCC	3 cut(s)	34, 1830, 2194
BcnI	CCSGG	3 cut(s)	305, 1720, 1824
BcoDI	GTCTC	5 cut(s)	129, 357, 606, 1661, 1717
BfaI	CTAG	3 cut(s)	870, 1458, 2227
BfmI	CTRYAG	1 cut(s)	335
BfoI	RGCGCY	1 cut(s)	1118
BfuI	GTATCC	3 cut(s)	34, 1830, 2194
BglI	GCCNNNNNGGC	1 cut(s)	440
BglII	AGATCT	2 cut(s)	382, 1125
Bme1390I	CCNGG	4 cut(s)	305, 1044, 1720, 1824
Bme18I	GGWCC	4 cut(s)	717, 886, 1240, 1477
BmeRI	GACNNNNNGTC	1 cut(s)	1867
BmeT110I	CYCGRG	1 cut(s)	1149
BmgT120I	GGNCC	4 cut(s)	717, 886, 1240, 1477
BmiI	GGNNCC	2 cut(s)	888, 1804
BmrFI	CCNGG	4 cut(s)	305, 1044, 1720, 1824
BmrI	ACTGGG	1 cut(s)	262
BmsI	GCATC	3 cut(s)	409, 765, 1144
BmuI	ACTGGG	1 cut(s)	262
BoxI	GACNNNNGTC	1 cut(s)	1631
BplI	GAGNNNNNCTC	2 cut(s)	1949, 1981
BpmI	CTGGAG	1 cut(s)	197
BpuMI	CCSGG	3 cut(s)	305, 1720, 1824
BsaAI	YACGTR	1 cut(s)	687
BsaI	GGTCTC	2 cut(s)	357, 1717
BsaJI	CCNNGG	4 cut(s)	36, 512, 1243, 1707
BsaWI	WCCGGW	2 cut(s)	767, 883
BsaXI	ACNNNNNCTCC	2 cut(s)	1344, 1374
Bsc4I	CCNNNNNNNGG	3 cut(s)	21, 1249, 1812
Bse118I	RCCGGY	1 cut(s)	767
Bse1I	ACTGG	4 cut(s)	9, 26, 268, 1981
Bse21I	CCTNAGG	1 cut(s)	372
Bse3DI	GCAATG	3 cut(s)	1029, 1335, 1377
BseBI	CCWGG	1 cut(s)	1044
BseDI	CCNNGG	4 cut(s)	36, 512, 1243, 1707
BseGI	GGATG	4 cut(s)	762, 787, 1582, 1917
BseLI	CCNNNNNNNGG	3 cut(s)	21, 1249, 1812
BseMI	GCAATG	3 cut(s)	1029, 1335, 1377
BseMII	CTCAG	4 cut(s)	377, 386, 596, 1563
BseNI	ACTGG	4 cut(s)	9, 26, 268, 1981
BseRI	GAGGAG	5 cut(s)	117, 237, 662, 851, 1268
BseSI	GKGCMC	1 cut(s)	1430
BseXI	GCAGC	9 cut(s)	220, 349, 430, 958, 1615, 1629, 1776, 1898, 2032
BseYI	CCCAGC	1 cut(s)	929
BsgI	GTGCAG	3 cut(s)	1814, 1881, 1905
Bsh1236I	CGCG	2 cut(s)	644, 1709
Bsh1285I	CGRYCG	1 cut(s)	741
BshFI	GGCC	4 cut(s)	308, 1034, 1195, 1306
BshTI	ACCGGT	1 cut(s)	767
BsiEI	CGRYCG	1 cut(s)	741
BsiHKAI	GWGCWC	6 cut(s)	330, 642, 675, 1413, 1430, 1783
BsiHKCI	CYCGRG	1 cut(s)	1149
BsiSI	CCGG	9 cut(s)	143, 305, 768, 779, 884, 1035, 1720, 1823, 1955
BslFI	GGGAC	2 cut(s)	535, 674
BslI	CCNNNNNNNGG	3 cut(s)	21, 1249, 1812
BsmAI	GTCTC	5 cut(s)	129, 357, 606, 1661, 1717
BsmFI	GGGAC	2 cut(s)	535, 674
BsmI	GAATGC	1 cut(s)	2014
BsnI	GGCC	4 cut(s)	308, 1034, 1195, 1306
Bso31I	GGTCTC	2 cut(s)	357, 1717
BsoBI	CYCGRG	1 cut(s)	1149
Bsp1286I	GDGCHC	7 cut(s)	330, 642, 675, 1150, 1413, 1430, 1783
Bsp1407I	TGTACA	1 cut(s)	1002
Bsp143I	GATC	3 cut(s)	382, 1125, 2092
Bsp19I	CCATGG	1 cut(s)	512
BspANI	GGCC	4 cut(s)	308, 1034, 1195, 1306
BspCNI	CTCAG	4 cut(s)	378, 385, 597, 1564
BspFNI	CGCG	2 cut(s)	644, 1709
BspHI	TCATGA	1 cut(s)	1783
BspLI	GGNNCC	2 cut(s)	888, 1804
BspMAI	CTGCAG	1 cut(s)	339
BspPI	GGATC	1 cut(s)	2100
BspQI	GCTCTTC	2 cut(s)	630, 859
BspTNI	GGTCTC	2 cut(s)	357, 1717
BsrBI	CCGCTC	3 cut(s)	114, 1350, 1970
BsrDI	GCAATG	3 cut(s)	1029, 1335, 1377
BsrFI	RCCGGY	1 cut(s)	767
BsrGI	TGTACA	1 cut(s)	1002
BsrI	ACTGG	4 cut(s)	9, 26, 268, 1981
BssAI	RCCGGY	1 cut(s)	767
BssECI	CCNNGG	4 cut(s)	36, 512, 1243, 1707
BssMI	GATC	3 cut(s)	382, 1125, 2092
BssSI	CACGAG	1 cut(s)	474
BssT1I	CCWWGG	2 cut(s)	512, 1243
Bst2BI	CACGAG	1 cut(s)	474
Bst2UI	CCWGG	1 cut(s)	1044
Bst4CI	ACNGT	3 cut(s)	416, 1313, 2146
Bst6I	CTCTTC	5 cut(s)	70, 630, 859, 1899, 1938
BstAUI	TGTACA	1 cut(s)	1002
BstBAI	YACGTR	1 cut(s)	687
BstC8I	GCNNGC	2 cut(s)	642, 1608
BstDEI	CTNAG	6 cut(s)	372, 386, 605, 845, 1263, 1572
BstDSI	CCRYGG	2 cut(s)	512, 1707
BstF5I	GGATG	4 cut(s)	762, 787, 1582, 1917
BstFNI	CGCG	2 cut(s)	644, 1709
BstH2I	RGCGCY	1 cut(s)	1118
BstHHI	GCGC	3 cut(s)	487, 1117, 2050
BstKTI	GATC	3 cut(s)	385, 1128, 2095
BstMAI	GTCTC	5 cut(s)	129, 357, 606, 1661, 1717
BstMBI	GATC	3 cut(s)	382, 1125, 2092
BstMCI	CGRYCG	1 cut(s)	741
BstNI	CCWGG	1 cut(s)	1044
BstNSI	RCATGY	1 cut(s)	532
BstPAI	GACNNNNGTC	1 cut(s)	1631
BstSCI	CCNGG	4 cut(s)	303, 1042, 1718, 1822
BstSFI	CTRYAG	1 cut(s)	335
BstSLI	GKGCMC	1 cut(s)	1430
BstSNI	TACGTA	1 cut(s)	687
BstUI	CGCG	2 cut(s)	644, 1709
BstV1I	GCAGC	9 cut(s)	220, 349, 430, 958, 1615, 1629, 1776, 1898, 2032
BstX2I	RGATCY	2 cut(s)	382, 1125
BstXI	CCANNNNNNTGG	1 cut(s)	1314
BstYI	RGATCY	2 cut(s)	382, 1125
Bsu36I	CCTNAGG	1 cut(s)	372
BsuI	GTATCC	3 cut(s)	34, 1830, 2194
BsuRI	GGCC	4 cut(s)	308, 1034, 1195, 1306
BtgI	CCRYGG	2 cut(s)	512, 1707
BtsCI	GGATG	4 cut(s)	762, 787, 1582, 1917
BtsI	GCAGTG	2 cut(s)	1323, 1893
BtsIMutI	CAGTG	4 cut(s)	16, 19, 1323, 1893
Cac8I	GCNNGC	2 cut(s)	642, 1608
CaiI	CAGNNNCTG	1 cut(s)	220
CciI	TCATGA	1 cut(s)	1783
CfoI	GCGC	3 cut(s)	487, 1117, 2050
Cfr10I	RCCGGY	1 cut(s)	767
Cfr13I	GGNCC	4 cut(s)	717, 886, 1240, 1477
Cfr42I	CCGCGG	1 cut(s)	1710
CseI	GACGC	1 cut(s)	1276
Csp6I	GTAC	4 cut(s)	1003, 1704, 1934, 2128
CspAI	ACCGGT	1 cut(s)	767
CspCI	CAANNNNNGTGG	2 cut(s)	412, 447
CviAII	CATG	6 cut(s)	513, 529, 1420, 1772, 1784, 2030
CviQI	GTAC	4 cut(s)	1003, 1704, 1934, 2128
DdeI	CTNAG	6 cut(s)	372, 386, 605, 845, 1263, 1572
DpnI	GATC	3 cut(s)	384, 1127, 2094
DpnII	GATC	3 cut(s)	382, 1125, 2092
DrdI	GACNNNNNNGTC	1 cut(s)	737
DriI	GACNNNNNGTC	1 cut(s)	1867
DseDI	GACNNNNNNGTC	1 cut(s)	737
EaeI	YGGCCR	1 cut(s)	306
Eam1104I	CTCTTC	5 cut(s)	70, 630, 859, 1899, 1938
Eam1105I	GACNNNNNGTC	1 cut(s)	1867
EarI	CTCTTC	5 cut(s)	70, 630, 859, 1899, 1938
EciI	GGCGGA	2 cut(s)	106, 240
Ecl136II	GAGCTC	4 cut(s)	640, 673, 1411, 1781
Eco105I	TACGTA	1 cut(s)	687
Eco130I	CCWWGG	2 cut(s)	512, 1243
Eco24I	GRGCYC	5 cut(s)	642, 675, 1150, 1413, 1783
Eco31I	GGTCTC	2 cut(s)	357, 1717
Eco32I	GATATC	1 cut(s)	276
Eco47I	GGWCC	4 cut(s)	717, 886, 1240, 1477
Eco53kI	GAGCTC	4 cut(s)	640, 673, 1411, 1781
Eco81I	CCTNAGG	1 cut(s)	372
Eco88I	CYCGRG	1 cut(s)	1149
EcoICRI	GAGCTC	4 cut(s)	640, 673, 1411, 1781
EcoRI	GAATTC	1 cut(s)	31
EcoRII	CCWGG	1 cut(s)	1042
EcoRV	GATATC	1 cut(s)	276
EcoT14I	CCWWGG	2 cut(s)	512, 1243
EcoT38I	GRGCYC	5 cut(s)	642, 675, 1150, 1413, 1783
ErhI	CCWWGG	2 cut(s)	512, 1243
FaeI	CATG	6 cut(s)	516, 532, 1423, 1775, 1787, 2033
FaqI	GGGAC	2 cut(s)	535, 674
FatI	CATG	6 cut(s)	512, 528, 1419, 1771, 1783, 2029
FauI	CCCGC	3 cut(s)	147, 474, 1932
FokI	GGATG	4 cut(s)	769, 794, 1589, 1924
FriOI	GRGCYC	5 cut(s)	642, 675, 1150, 1413, 1783
FspBI	CTAG	3 cut(s)	870, 1458, 2227
GlaI	GCGC	3 cut(s)	486, 1116, 2049
GsaI	CCCAGC	1 cut(s)	933
GsuI	CTGGAG	1 cut(s)	197
HaeII	RGCGCY	1 cut(s)	1118
HaeIII	GGCC	4 cut(s)	308, 1034, 1195, 1306
HapII	CCGG	9 cut(s)	143, 305, 768, 779, 884, 1035, 1720, 1823, 1955
HgaI	GACGC	1 cut(s)	1276
HhaI	GCGC	3 cut(s)	487, 1117, 2050
Hin1II	CATG	6 cut(s)	516, 532, 1423, 1775, 1787, 2033
Hin6I	GCGC	3 cut(s)	485, 1115, 2048
HinP1I	GCGC	3 cut(s)	485, 1115, 2048
HincII	GTYRAC	1 cut(s)	301
HindII	GTYRAC	1 cut(s)	301
HindIII	AAGCTT	1 cut(s)	722
HinfI	GANTC	9 cut(s)	25, 359, 493, 1079, 1438, 1464, 1627, 1715, 2062
HpaII	CCGG	9 cut(s)	143, 305, 768, 779, 884, 1035, 1720, 1823, 1955
HphI	GGTGA	6 cut(s)	60, 388, 556, 799, 1481, 1636
Hpy166II	GTNNAC	4 cut(s)	19, 301, 1003, 1428
Hpy188III	TCNNGA	5 cut(s)	356, 613, 1151, 1687, 1784
Hpy8I	GTNNAC	4 cut(s)	19, 301, 1003, 1428
HpyAV	CCTTC	3 cut(s)	445, 1024, 1738
HpyCH4III	ACNGT	3 cut(s)	416, 1313, 2146
HpyCH4IV	ACGT	4 cut(s)	686, 1491, 1863, 2164
HpyF3I	CTNAG	6 cut(s)	372, 386, 605, 845, 1263, 1572
HpySE526I	ACGT	4 cut(s)	686, 1491, 1863, 2164
Hsp92II	CATG	6 cut(s)	516, 532, 1423, 1775, 1787, 2033
HspAI	GCGC	3 cut(s)	485, 1115, 2048
KspI	CCGCGG	1 cut(s)	1710
Kzo9I	GATC	3 cut(s)	382, 1125, 2092
LguI	GCTCTTC	2 cut(s)	630, 859
LmnI	GCTCC	7 cut(s)	119, 216, 326, 1056, 1171, 1294, 1347
Lsp1109I	GCAGC	9 cut(s)	220, 349, 430, 958, 1615, 1629, 1776, 1898, 2032
LweI	GCATC	3 cut(s)	409, 765, 1144
MaeI	CTAG	3 cut(s)	870, 1458, 2227
MaeII	ACGT	4 cut(s)	686, 1491, 1863, 2164
MaeIII	GTNAC	6 cut(s)	589, 787, 1319, 1624, 1869, 1988
MalI	GATC	3 cut(s)	384, 1127, 2094
MbiI	CCGCTC	3 cut(s)	114, 1350, 1970
MboI	GATC	3 cut(s)	382, 1125, 2092
MfeI	CAATTG	1 cut(s)	538
MflI	RGATCY	2 cut(s)	382, 1125
MhlI	GDGCHC	7 cut(s)	330, 642, 675, 1150, 1413, 1430, 1783
MlyI	GAGTC	4 cut(s)	19, 353, 1621, 2071
MmeI	TCCRAC	3 cut(s)	264, 1272, 1295
MseI	TTAA	2 cut(s)	1512, 1674
MslI	CAYNNNNRTG	1 cut(s)	1328
MspA1I	CMGCKG	3 cut(s)	340, 1709, 1939
MspI	CCGG	9 cut(s)	143, 305, 768, 779, 884, 1035, 1720, 1823, 1955
MspR9I	CCNGG	4 cut(s)	305, 1044, 1720, 1824
MunI	CAATTG	1 cut(s)	538
Mva1269I	GAATGC	1 cut(s)	2014
MvaI	CCWGG	1 cut(s)	1044
MvnI	CGCG	2 cut(s)	644, 1709
NciI	CCSGG	3 cut(s)	305, 1720, 1824
NcoI	CCATGG	1 cut(s)	512
NdeII	GATC	3 cut(s)	382, 1125, 2092
NlaIII	CATG	6 cut(s)	516, 532, 1423, 1775, 1787, 2033
NlaIV	GGNNCC	2 cut(s)	888, 1804
NmuCI	GTSAC	5 cut(s)	589, 787, 1624, 1869, 1988
NspI	RCATGY	1 cut(s)	532
PaeR7I	CTCGAG	1 cut(s)	1149
PagI	TCATGA	1 cut(s)	1783
PciI	ACATGT	1 cut(s)	528
PciSI	GCTCTTC	2 cut(s)	630, 859
PctI	GAATGC	1 cut(s)	2014
PfeI	GAWTC	5 cut(s)	493, 1079, 1438, 1464, 1715
PflFI	GACNNNGTC	1 cut(s)	2061
PfoI	TCCNGGA	1 cut(s)	1718
PinAI	ACCGGT	1 cut(s)	767
PleI	GAGTC	4 cut(s)	19, 353, 1621, 2070
PpsI	GAGTC	4 cut(s)	19, 353, 1621, 2070
Ppu21I	YACGTR	1 cut(s)	687
PscI	ACATGT	1 cut(s)	528
PshAI	GACNNNNGTC	1 cut(s)	1631
Psp124BI	GAGCTC	4 cut(s)	642, 675, 1413, 1783
Psp1406I	AACGTT	1 cut(s)	2164
Psp6I	CCWGG	1 cut(s)	1042
PspFI	CCCAGC	1 cut(s)	929
PspGI	CCWGG	1 cut(s)	1042
PspN4I	GGNNCC	2 cut(s)	888, 1804
PspPI	GGNCC	4 cut(s)	717, 886, 1240, 1477
PstI	CTGCAG	1 cut(s)	339
PstNI	CAGNNNCTG	1 cut(s)	220
PsuI	RGATCY	2 cut(s)	382, 1125
PsyI	GACNNNGTC	1 cut(s)	2061
RsaI	GTAC	4 cut(s)	1004, 1705, 1935, 2129
RsaNI	GTAC	4 cut(s)	1003, 1704, 1934, 2128
RseI	CAYNNNNRTG	1 cut(s)	1328
SacI	GAGCTC	4 cut(s)	642, 675, 1413, 1783
SacII	CCGCGG	1 cut(s)	1710
SapI	GCTCTTC	2 cut(s)	630, 859
SaqAI	TTAA	2 cut(s)	1512, 1674
Sau3AI	GATC	3 cut(s)	382, 1125, 2092
Sau96I	GGNCC	4 cut(s)	717, 886, 1240, 1477
SchI	GAGTC	4 cut(s)	19, 353, 1621, 2071
ScrFI	CCNGG	4 cut(s)	305, 1044, 1720, 1824
SduI	GDGCHC	7 cut(s)	330, 642, 675, 1150, 1413, 1430, 1783
SfaNI	GCATC	3 cut(s)	409, 765, 1144
SfcI	CTRYAG	1 cut(s)	335
Sfr274I	CTCGAG	1 cut(s)	1149
Sfr303I	CCGCGG	1 cut(s)	1710
SgrBI	CCGCGG	1 cut(s)	1710
SinI	GGWCC	4 cut(s)	717, 886, 1240, 1477
SlaI	CTCGAG	1 cut(s)	1149
SmiMI	CAYNNNNRTG	1 cut(s)	1328
SmlI	CTYRAG	1 cut(s)	1149
SmoI	CTYRAG	1 cut(s)	1149
SnaBI	TACGTA	1 cut(s)	687
SspMI	CTAG	3 cut(s)	870, 1458, 2227
SstI	GAGCTC	4 cut(s)	642, 675, 1413, 1783
StyD4I	CCNGG	4 cut(s)	303, 1042, 1718, 1822
StyI	CCWWGG	2 cut(s)	512, 1243
TaaI	ACNGT	3 cut(s)	416, 1313, 2146
TaiI	ACGT	4 cut(s)	689, 1494, 1866, 2167
TatI	WGTACW	2 cut(s)	1002, 1933
TauI	GCSGC	2 cut(s)	114, 343
TfiI	GAWTC	5 cut(s)	493, 1079, 1438, 1464, 1715
Tru1I	TTAA	2 cut(s)	1512, 1674
Tru9I	TTAA	2 cut(s)	1512, 1674
TscAI	CASTG	4 cut(s)	16, 26, 1330, 1900
TseFI	GTSAC	5 cut(s)	589, 787, 1624, 1869, 1988
TseI	GCWGC	9 cut(s)	208, 337, 443, 971, 1603, 1642, 1789, 1886, 2045
Tsp45I	GTSAC	5 cut(s)	589, 787, 1624, 1869, 1988
TspDTI	ATGAA	4 cut(s)	611, 1195, 1298, 1388
TspGWI	ACGGA	1 cut(s)	313
TspRI	CASTG	4 cut(s)	16, 26, 1330, 1900
Tth111I	GACNNNGTC	1 cut(s)	2061
VneI	GTGCAC	1 cut(s)	1426
VpaK11BI	GGWCC	4 cut(s)	717, 886, 1240, 1477
XapI	RAATTY	4 cut(s)	31, 95, 556, 1994
XceI	RCATGY	1 cut(s)	532
XhoI	CTCGAG	1 cut(s)	1149
XspI	CTAG	3 cut(s)	870, 1458, 2227

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.