Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0010049

Physical Location & Seq

Genomic Coordinates Reverse (-)

27115675 .. 27123476

Gene Structure 7802 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1629 bp

ATGGGTCGCAAACTCAAAGAATCCTCCGCCGCCGTCGGCGAGGCAGAACTGCAACAGACCCAACCTCTCCACGAAACCAAAGCGGAGAGGAAGAAGAAGAAGCAGAAAAACAAAGACGAAGAAGAAGCCCATCCCAGCCCCGACCCCAAGAGAAAGCTCGAAGACATTGCTCCTCAGGAAGAAGACTCAGATTCTATTGTCAAAAAGAAGAAGAAGAAGAAGAAGCACAAGAAATCCAAAGACAAGGAGGAAGAACCCGAAAATAATCAGGAGCCACAGGAAAATGGCCATGCCGACGAAGGTGAATCTGGAGACGCGGCTGTGGTGGTTAGTGGGAAGGATTCGAAGGAAGCCAAATACAAGCCACTGACTTCCTTTGCAGAATCGAAGCTTCCTAGTGAGGTTTTACAATGCTGTCAGAATTTCAACAGTCCATCTCCGATTCAATCCCGGGCCTGGCCGTTCTTATTGGACGGTCGCGATTTTATTGGGATTGCAAAAACCGGGTCAGGCAAGACTTTGGCTTTTGGAATACCCGCTATTATGCAGGTTTTGAACAAGAGGAAGGGTAAGTTTTCGAGGGGACGAACTCCTCTTTGCCTTGCACTTGCACCTACAAGGGAGCTAGCTCAACAAATTTCAGAGGTTTTGTTCGAGGCTGGGAAACCTTGTGGTGTGGTATCAGTTTGTTTGTTCGGAGGAGCATCCAAAGGACCCCAAATATCTATGTTGAAGTCTGGTGTTGACATTGTCATTGGAACTCCTGGTCGTCTGAAGGATCTCATTGAAAATAAGGATTGTTGCCTGAAGGAAGTATCTTTTATGGTCCTTGATGAAGCAGATCGAATGCTAGACATGGGATTTGAACCAGAAGTTCGCTATATATTGGGCCAGACATGCTCTGCTCGGCAAATGGTAATGTTCAGTGCTACATGGCCTCCATCAGTTCATCAATTAGCTCAGGAATTCATGGATCCTAATCCTGTTAAGGTAGTTGTTGGATCAGAGGATTTAGCTGCCAACCATGATGTTATGCAGATAGTTGAGGTCTTGGATGAGCATTCACGTAGTAGGCGCTTGGTTGCATTGCTAGAAAAATACCACAAATCGCAAGAGAACAGAATTTTGATTTTTGTCTTGTACAAAGCGGAAGCCCCTCACCTTGAGAATATGCTGCACAAAGGGGGTTGGAAGGCTGTTTCTATACATGGTGATAAGGCACAAAGTGAGCGTACGAAGGCACTCTCTCTATTCAAGAAGGGAAGCTGCCCCTTGATGATAGCTACTGATGTGGCTGCTCGTGGATTGGATATTCCAGACGTTGAAGTAGTGATCAACTATAGTTTTCCCCTCACTACTGAGGATTATGTCCACCGAATTGGGCGGACTGGGCGGGCTGGAAAGAAGGGCGTTGCTCATACTTTTTTCACACATCACAACAAGGGACTTGCGGGGGAGCTGGTCAATGTTCTCAGAGAAGCCAAGCAAAATGTGCCAGAAGCCTTGTTGAAATTTGGCACCCATGTTAAGAAAAAGGAGTCCAAACTTTACGGGGCTCACTTCAAAGAGATTCCTGCCGATGCTCCAAAGTCTCAAAAAATCACCTTCAACTCTGACGATGAAGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

543

Amino Acids

60.09

Weight (kDa)

8.68

Isoelectric Point (pI)

39.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	146 - 318	3e-46	DEAD/DEAH box helicase
Helicase_C	PF00271	360 - 467	1.4e-30	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010049)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G31970
fragaria_vesca	FvH4_5g23490
malus_domestica	MD00G1068700.v1.1 MD06G1023800.v1.1
prunus_persica	Prupe.5G024900_v2.0.a1
pyrus_communis	pycom16g25290
rosa_chinensis	RchiOBHm_Chr7g0212761
rosa_laevigata	RLG00000002870
rosa_multiflora	Rmu_sc0000394.1_g000033 Rmu_sc0008336.1_g000001
rosa_roxburghii	Rroxscaffold_3G00246570 Rroxscaffold_3G00250790 Rroxscaffold_4G00321740 Rroxscaffold_4G00321750
rosa_rugosa	Rorug07G0136500
rosa_samantha	Rh7BG263500 Rh7CG287100
rosa_wichuraiana	Rw7G023130 Rw7G023160 Rw7G023230 Rw7G023270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	538
AccB1I	GGYRCC	1 cut(s)	1517
AccII	CGCG	2 cut(s)	317, 480
AciI	CCGC	9 cut(s)	27, 30, 83, 317, 537, 1148, 1384, 1393, 1451
AclWI	GGATC	4 cut(s)	786, 968, 981, 1009
AcoI	YGGCCR	2 cut(s)	286, 458
AcsI	RAATTY	5 cut(s)	421, 636, 965, 1122, 1511
AcuI	CTGAAG	2 cut(s)	794, 827
AdeI	CACNNNGTG	1 cut(s)	1226
AfaI	GTAC	2 cut(s)	1142, 1234
AfiI	CCNNNNNNNGG	1 cut(s)	456
AjnI	CCWGG	2 cut(s)	455, 763
AluBI	AGCT	9 cut(s)	157, 391, 625, 629, 959, 1016, 1266, 1283, 1459
AluI	AGCT	9 cut(s)	157, 391, 625, 629, 959, 1016, 1266, 1283, 1459
Alw26I	GTCTC	2 cut(s)	306, 1596
AlwI	GGATC	4 cut(s)	786, 968, 981, 1009
Ama87I	CYCGRG	1 cut(s)	450
AoxI	GGCC	5 cut(s)	286, 453, 458, 889, 935
ApeKI	GCWGC	4 cut(s)	1016, 1174, 1266, 1295
ApoI	RAATTY	5 cut(s)	421, 636, 965, 1122, 1511
ArsI	GACNNNNNNTTYG	4 cut(s)	491, 523, 845, 877
AspLEI	GCGC	1 cut(s)	1077
AspS9I	GGNCC	4 cut(s)	453, 713, 826, 889
AsuC2I	CCSGG	3 cut(s)	451, 452, 505
AsuHPI	GGTGA	4 cut(s)	314, 1151, 1223, 1594
AsuII	TTCGAA	1 cut(s)	344
AsuNHI	GCTAGC	1 cut(s)	625
AvaI	CYCGRG	1 cut(s)	450
AvaII	GGWCC	2 cut(s)	713, 826
AxyI	CCTNAGG	1 cut(s)	174
BalI	TGGCCA	1 cut(s)	288
BamHI	GGATCC	1 cut(s)	973
BanI	GGYRCC	1 cut(s)	1517
BanII	GRGCYC	1 cut(s)	1558
BauI	CACGAG	1 cut(s)	1299
BbsI	GAAGAC	2 cut(s)	168, 189
BbvI	GCAGC	4 cut(s)	1003, 1161, 1253, 1282
BccI	CCATC	3 cut(s)	138, 442, 949
BceAI	ACGGC	2 cut(s)	17, 445
BcgI	CGANNNNNNTGC	2 cut(s)	149, 183
BciT130I	CCWGG	2 cut(s)	457, 765
BclI	TGATCA	1 cut(s)	1332
BcnI	CCSGG	3 cut(s)	451, 452, 505
BcoDI	GTCTC	2 cut(s)	306, 1596
BfaI	CTAG	4 cut(s)	396, 626, 851, 1091
BfmI	CTRYAG	1 cut(s)	1339
BfoI	RGCGCY	1 cut(s)	1078
BfuAI	ACCTGC	1 cut(s)	538
BisI	GCNGC	6 cut(s)	30, 318, 1017, 1175, 1267, 1296
BlsI	GCNGC	6 cut(s)	31, 319, 1018, 1176, 1268, 1297
Bme1390I	CCNGG	5 cut(s)	451, 452, 457, 505, 765
Bme18I	GGWCC	2 cut(s)	713, 826
BmeT110I	CYCGRG	1 cut(s)	450
BmgT120I	GGNCC	4 cut(s)	453, 713, 826, 889
BmiI	GGNNCC	4 cut(s)	273, 715, 975, 1519
BmrFI	CCNGG	5 cut(s)	451, 452, 457, 505, 765
BmrI	ACTGGG	1 cut(s)	1398
BmsI	GCATC	2 cut(s)	713, 1570
BmtI	GCTAGC	1 cut(s)	629
BmuI	ACTGGG	1 cut(s)	1398
BpiI	GAAGAC	2 cut(s)	168, 189
BpmI	CTGGAG	1 cut(s)	330
Bpu10I	CCTNAGC	1 cut(s)	960
Bpu14I	TTCGAA	1 cut(s)	344
BpuEI	CTTGAG	1 cut(s)	1184
BpuMI	CCSGG	3 cut(s)	451, 452, 505
BsaAI	YACGTR	1 cut(s)	1067
BsaBI	GATNNNNATC	1 cut(s)	978
BsaJI	CCNNGG	1 cut(s)	450
BsaXI	ACNNNNNCTCC	2 cut(s)	922, 952
Bsc4I	CCNNNNNNNGG	1 cut(s)	456
Bse1I	ACTGG	1 cut(s)	1393
Bse21I	CCTNAGG	1 cut(s)	174
Bse3DI	GCAATG	2 cut(s)	165, 1085
Bse8I	GATNNNNATC	1 cut(s)	978
BseBI	CCWGG	2 cut(s)	457, 765
BseDI	CCNNGG	1 cut(s)	450
BseGI	GGATG	3 cut(s)	130, 704, 1060
BseJI	GATNNNNATC	1 cut(s)	978
BseLI	CCNNNNNNNGG	1 cut(s)	456
BseMI	GCAATG	2 cut(s)	165, 1085
BseMII	CTCAG	5 cut(s)	188, 201, 974, 1350, 1486
BseNI	ACTGG	1 cut(s)	1393
BseRI	GAGGAG	3 cut(s)	162, 582, 714
BseXI	GCAGC	4 cut(s)	1003, 1161, 1253, 1282
BseYI	CCCAGC	2 cut(s)	134, 659
BsgI	GTGCAG	1 cut(s)	1160
Bsh1236I	CGCG	2 cut(s)	317, 480
Bsh1285I	CGRYCG	1 cut(s)	478
BshFI	GGCC	5 cut(s)	288, 455, 460, 891, 937
BshNI	GGYRCC	1 cut(s)	1517
BsiEI	CGRYCG	1 cut(s)	478
BsiHKCI	CYCGRG	1 cut(s)	450
BsiSI	CCGG	2 cut(s)	451, 504
BsiWI	CGTACG	1 cut(s)	1232
BslFI	GGGAC	2 cut(s)	597, 1458
BslI	CCNNNNNNNGG	1 cut(s)	456
BsmAI	GTCTC	2 cut(s)	306, 1596
BsmBI	CGTCTC	1 cut(s)	306
BsmFI	GGGAC	2 cut(s)	597, 1458
BsmI	GAATGC	2 cut(s)	852, 1060
BsnI	GGCC	5 cut(s)	288, 455, 460, 891, 937
BsoBI	CYCGRG	1 cut(s)	450
Bsp119I	TTCGAA	1 cut(s)	344
Bsp1286I	GDGCHC	1 cut(s)	1558
Bsp1407I	TGTACA	1 cut(s)	1140
Bsp143I	GATC	5 cut(s)	778, 841, 973, 1001, 1332
Bsp68I	TCGCGA	1 cut(s)	480
BspACI	CCGC	9 cut(s)	27, 30, 83, 317, 537, 1148, 1384, 1393, 1451
BspANI	GGCC	5 cut(s)	288, 455, 460, 891, 937
BspCNI	CTCAG	5 cut(s)	187, 200, 973, 1351, 1485
BspFNI	CGCG	2 cut(s)	317, 480
BspLI	GGNNCC	4 cut(s)	273, 715, 975, 1519
BspMI	ACCTGC	1 cut(s)	538
BspOI	GCTAGC	1 cut(s)	629
BspPI	GGATC	4 cut(s)	786, 968, 981, 1009
BspT104I	TTCGAA	1 cut(s)	344
BspT107I	GGYRCC	1 cut(s)	1517
BsrDI	GCAATG	2 cut(s)	165, 1085
BsrGI	TGTACA	1 cut(s)	1140
BsrI	ACTGG	1 cut(s)	1393
BssECI	CCNNGG	1 cut(s)	450
BssMI	GATC	5 cut(s)	778, 841, 973, 1001, 1332
BssSI	CACGAG	1 cut(s)	1299
Bst2BI	CACGAG	1 cut(s)	1299
Bst2UI	CCWGG	2 cut(s)	457, 765
Bst4CI	ACNGT	2 cut(s)	431, 476
BstAPI	GCANNNNNTGC	1 cut(s)	1492
BstAUI	TGTACA	1 cut(s)	1140
BstBAI	YACGTR	1 cut(s)	1067
BstBI	TTCGAA	1 cut(s)	344
BstC8I	GCNNGC	2 cut(s)	627, 1395
BstDEI	CTNAG	5 cut(s)	174, 187, 960, 1359, 1472
BstF5I	GGATG	3 cut(s)	130, 704, 1060
BstFNI	CGCG	2 cut(s)	317, 480
BstH2I	RGCGCY	1 cut(s)	1078
BstHHI	GCGC	1 cut(s)	1077
BstKTI	GATC	5 cut(s)	781, 844, 976, 1004, 1335
BstMAI	GTCTC	2 cut(s)	306, 1596
BstMBI	GATC	5 cut(s)	778, 841, 973, 1001, 1332
BstMCI	CGRYCG	1 cut(s)	478
BstMWI	GCNNNNNNNGC	3 cut(s)	897, 1390, 1492
BstNI	CCWGG	2 cut(s)	457, 765
BstNSI	RCATGY	1 cut(s)	900
BstSCI	CCNGG	5 cut(s)	449, 450, 455, 503, 763
BstSFI	CTRYAG	1 cut(s)	1339
BstUI	CGCG	2 cut(s)	317, 480
BstV1I	GCAGC	4 cut(s)	1003, 1161, 1253, 1282
BstV2I	GAAGAC	2 cut(s)	168, 189
BstX2I	RGATCY	2 cut(s)	778, 973
BstXI	CCANNNNNNTGG	1 cut(s)	1379
BstYI	RGATCY	2 cut(s)	778, 973
Bsu36I	CCTNAGG	1 cut(s)	174
BsuRI	GGCC	5 cut(s)	288, 455, 460, 891, 937
BtsCI	GGATG	3 cut(s)	130, 704, 1060
BtsIMutI	CAGTG	2 cut(s)	365, 931
BtuMI	TCGCGA	1 cut(s)	480
BveI	ACCTGC	1 cut(s)	538
Cac8I	GCNNGC	2 cut(s)	627, 1395
CfoI	GCGC	1 cut(s)	1077
Cfr13I	GGNCC	4 cut(s)	453, 713, 826, 889
Cfr9I	CCCGGG	1 cut(s)	450
CseI	GACGC	1 cut(s)	323
Csp6I	GTAC	2 cut(s)	1141, 1233
CviAII	CATG	8 cut(s)	290, 856, 897, 933, 970, 1025, 1208, 1523
CviQI	GTAC	2 cut(s)	1141, 1233
DdeI	CTNAG	5 cut(s)	174, 187, 960, 1359, 1472
DpnI	GATC	5 cut(s)	780, 843, 975, 1003, 1334
DpnII	GATC	5 cut(s)	778, 841, 973, 1001, 1332
DraIII	CACNNNGTG	1 cut(s)	1226
EaeI	YGGCCR	2 cut(s)	286, 458
EciI	GGCGGA	2 cut(s)	16, 1399
Eco24I	GRGCYC	1 cut(s)	1558
Eco47I	GGWCC	2 cut(s)	713, 826
Eco57I	CTGAAG	2 cut(s)	794, 827
Eco81I	CCTNAGG	1 cut(s)	174
Eco88I	CYCGRG	1 cut(s)	450
EcoO109I	RGGNCCY	1 cut(s)	713
EcoRI	GAATTC	1 cut(s)	965
EcoRII	CCWGG	2 cut(s)	455, 763
EcoT38I	GRGCYC	1 cut(s)	1558
Esp3I	CGTCTC	1 cut(s)	306
FaeI	CATG	8 cut(s)	293, 859, 900, 936, 973, 1028, 1211, 1526
FaqI	GGGAC	2 cut(s)	597, 1458
FatI	CATG	8 cut(s)	289, 855, 896, 932, 969, 1024, 1207, 1522
FauI	CCCGC	3 cut(s)	544, 1386, 1444
FbaI	TGATCA	1 cut(s)	1332
Fnu4HI	GCNGC	6 cut(s)	30, 318, 1017, 1175, 1267, 1296
FokI	GGATG	3 cut(s)	117, 691, 1067
FriOI	GRGCYC	1 cut(s)	1558
Fsp4HI	GCNGC	6 cut(s)	30, 318, 1017, 1175, 1267, 1296
FspBI	CTAG	4 cut(s)	396, 626, 851, 1091
GlaI	GCGC	1 cut(s)	1076
GluI	GCNGC	6 cut(s)	30, 318, 1017, 1175, 1267, 1296
GsaI	CCCAGC	2 cut(s)	138, 663
GsuI	CTGGAG	1 cut(s)	330
HaeII	RGCGCY	1 cut(s)	1078
HaeIII	GGCC	5 cut(s)	288, 455, 460, 891, 937
HapII	CCGG	2 cut(s)	451, 504
HgaI	GACGC	1 cut(s)	323
HhaI	GCGC	1 cut(s)	1077
Hin1II	CATG	8 cut(s)	293, 859, 900, 936, 973, 1028, 1211, 1526
Hin6I	GCGC	1 cut(s)	1075
HinP1I	GCGC	1 cut(s)	1075
HincII	GTYRAC	1 cut(s)	745
HindII	GTYRAC	1 cut(s)	745
HindIII	AAGCTT	1 cut(s)	389
HinfI	GANTC	9 cut(s)	20, 185, 191, 305, 341, 383, 442, 1538, 1570
HpaII	CCGG	2 cut(s)	451, 504
HphI	GGTGA	4 cut(s)	314, 1151, 1223, 1594
Hpy166II	GTNNAC	2 cut(s)	745, 1372
Hpy188I	TCNGA	9 cut(s)	190, 420, 441, 643, 698, 774, 1006, 1475, 1615
Hpy188III	TCNNGA	7 cut(s)	176, 269, 309, 479, 962, 1255, 1316
Hpy8I	GTNNAC	2 cut(s)	745, 1372
Hpy99I	CGWCG	2 cut(s)	38, 299
HpyCH4III	ACNGT	2 cut(s)	431, 476
HpyCH4IV	ACGT	2 cut(s)	1066, 1320
HpyCH4V	TGCA	9 cut(s)	52, 380, 497, 547, 605, 611, 1036, 1085, 1177
HpyF10VI	GCNNNNNNNGC	3 cut(s)	897, 1390, 1492
HpyF3I	CTNAG	5 cut(s)	174, 187, 960, 1359, 1472
HpySE526I	ACGT	2 cut(s)	1066, 1320
Hsp92II	CATG	8 cut(s)	293, 859, 900, 936, 973, 1028, 1211, 1526
HspAI	GCGC	1 cut(s)	1075
Ksp22I	TGATCA	1 cut(s)	1332
Kzo9I	GATC	5 cut(s)	778, 841, 973, 1001, 1332
LmnI	GCTCC	6 cut(s)	175, 271, 622, 701, 1456, 1588
Lsp1109I	GCAGC	4 cut(s)	1003, 1161, 1253, 1282
LweI	GCATC	2 cut(s)	713, 1570
MaeI	CTAG	4 cut(s)	396, 626, 851, 1091
MaeII	ACGT	2 cut(s)	1066, 1320
MalI	GATC	5 cut(s)	780, 843, 975, 1003, 1334
MboI	GATC	5 cut(s)	778, 841, 973, 1001, 1332
MflI	RGATCY	2 cut(s)	778, 973
MhlI	GDGCHC	1 cut(s)	1558
MlsI	TGGCCA	1 cut(s)	288
MluCI	AATT	7 cut(s)	421, 636, 953, 965, 1122, 1377, 1511
MluNI	TGGCCA	1 cut(s)	288
MlyI	GAGTC	2 cut(s)	179, 1547
MmeI	TCCRAC	2 cut(s)	979, 1169
Mox20I	TGGCCA	1 cut(s)	288
MscI	TGGCCA	1 cut(s)	288
MseI	TTAA	2 cut(s)	987, 1527
Msp20I	TGGCCA	1 cut(s)	288
MspI	CCGG	2 cut(s)	451, 504
MspR9I	CCNGG	5 cut(s)	451, 452, 457, 505, 765
Mva1269I	GAATGC	2 cut(s)	852, 1060
MvaI	CCWGG	2 cut(s)	457, 765
MvnI	CGCG	2 cut(s)	317, 480
MwoI	GCNNNNNNNGC	3 cut(s)	897, 1390, 1492
NciI	CCSGG	3 cut(s)	451, 452, 505
NdeII	GATC	5 cut(s)	778, 841, 973, 1001, 1332
NheI	GCTAGC	1 cut(s)	625
NlaIII	CATG	8 cut(s)	293, 859, 900, 936, 973, 1028, 1211, 1526
NlaIV	GGNNCC	4 cut(s)	273, 715, 975, 1519
NmeAIII	GCCGAG	1 cut(s)	886
NruI	TCGCGA	1 cut(s)	480
NspI	RCATGY	1 cut(s)	900
NspV	TTCGAA	1 cut(s)	344
PctI	GAATGC	2 cut(s)	852, 1060
PfeI	GAWTC	7 cut(s)	20, 191, 305, 341, 383, 442, 1570
Pfl23II	CGTACG	1 cut(s)	1232
PflFI	GACNNNGTC	1 cut(s)	749
PkrI	GCNGC	6 cut(s)	31, 319, 1018, 1176, 1268, 1297
PleI	GAGTC	2 cut(s)	179, 1546
PpsI	GAGTC	2 cut(s)	179, 1546
Ppu21I	YACGTR	1 cut(s)	1067
PpuMI	RGGWCCY	1 cut(s)	713
Psp5II	RGGWCCY	1 cut(s)	713
Psp6I	CCWGG	2 cut(s)	455, 763
PspFI	CCCAGC	2 cut(s)	134, 659
PspGI	CCWGG	2 cut(s)	455, 763
PspLI	CGTACG	1 cut(s)	1232
PspN4I	GGNNCC	4 cut(s)	273, 715, 975, 1519
PspPI	GGNCC	4 cut(s)	453, 713, 826, 889
PspPPI	RGGWCCY	1 cut(s)	713
PsuI	RGATCY	2 cut(s)	778, 973
PsyI	GACNNNGTC	1 cut(s)	749
RruI	TCGCGA	1 cut(s)	480
RsaI	GTAC	2 cut(s)	1142, 1234
RsaNI	GTAC	2 cut(s)	1141, 1233
SaqAI	TTAA	2 cut(s)	987, 1527
SatI	GCNGC	6 cut(s)	30, 318, 1017, 1175, 1267, 1296
Sau3AI	GATC	5 cut(s)	778, 841, 973, 1001, 1332
Sau96I	GGNCC	4 cut(s)	453, 713, 826, 889
SchI	GAGTC	2 cut(s)	179, 1547
ScrFI	CCNGG	5 cut(s)	451, 452, 457, 505, 765
SduI	GDGCHC	1 cut(s)	1558
SfaNI	GCATC	2 cut(s)	713, 1570
SfcI	CTRYAG	1 cut(s)	1339
SfuI	TTCGAA	1 cut(s)	344
SinI	GGWCC	2 cut(s)	713, 826
SmaI	CCCGGG	1 cut(s)	452
SmlI	CTYRAG	1 cut(s)	1163
SmoI	CTYRAG	1 cut(s)	1163
Sse9I	AATT	7 cut(s)	421, 636, 953, 965, 1122, 1377, 1511
SsiI	CCGC	9 cut(s)	27, 30, 83, 317, 537, 1148, 1384, 1393, 1451
SspMI	CTAG	4 cut(s)	396, 626, 851, 1091
StyD4I	CCNGG	5 cut(s)	449, 450, 455, 503, 763
TaaI	ACNGT	2 cut(s)	431, 476
TaiI	ACGT	2 cut(s)	1069, 1323
TaqI	TCGA	6 cut(s)	159, 344, 386, 578, 654, 844
TasI	AATT	7 cut(s)	421, 636, 953, 965, 1122, 1377, 1511
TatI	WGTACW	1 cut(s)	1140
TauI	GCSGC	2 cut(s)	32, 320
TfiI	GAWTC	7 cut(s)	20, 191, 305, 341, 383, 442, 1570
Tru1I	TTAA	2 cut(s)	987, 1527
Tru9I	TTAA	2 cut(s)	987, 1527
TscAI	CASTG	2 cut(s)	372, 931
TseI	GCWGC	4 cut(s)	1016, 1174, 1266, 1295
TspDTI	ATGAA	3 cut(s)	849, 938, 958
TspMI	CCCGGG	1 cut(s)	450
TspRI	CASTG	2 cut(s)	372, 931
Tth111I	GACNNNGTC	1 cut(s)	749
VpaK11BI	GGWCC	2 cut(s)	713, 826
XapI	RAATTY	5 cut(s)	421, 636, 965, 1122, 1511
XceI	RCATGY	1 cut(s)	900
XmaI	CCCGGG	1 cut(s)	450
XspI	CTAG	4 cut(s)	396, 626, 851, 1091

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.