pycom16g25480

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0012472
Physical Location & Seq
Forward (+)
27442622 .. 27444725
2104 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 771 bp
ATGAAGCATTCTATTTATAGAACAACTTCAAACAACAAAAAAAAAAAAAAAAAAAAAAAAAATTCCTCTTCTTCTTGTGTTCAGTGCCCTTACTGGGGTTTCCATTCACCGGATAAATGGTTCCGGTGGCTGGACCTTTTGCAGAAAACTCCACGAATTCCCTTTAATTTACGCCAAGTACCAAGCTTAATGGATTCTGAGGAGGTCTCTGCGAAGGGCAGCGTGGCCAATTTAATACTCCGGTGGTCTCTGGGTCTCCTTCTCCTTATCGTTGCTTTCTTTTCACTATCGCTGTTGGTCGCCTTCGTCGCCGTTTTCGTCGCCAATTCTTCAATTCCGAGCTCAATTTCAGTGTCCTCTCAGTGCAGGATCGTCTCAAGCAGTGTTGATCTTAAGTCATCTAAAGTGTGTGAGCTTGGACTGTTCAATTATAAAGCCAAGCATGTGTTTTATCCTTTTCAAGGAAGAAGGTTTCGATGCCGTTATGATTACTACTGGGCTTCAATTTTTAAGGTGGAATACAAAGATCGATCTTCTGGTCAGACTCAGCTTGCATTGGCTGAGGCTCCAAATGAGGCCCTTCCTCTTAATTGCAGACCTAATTTTGGTGCTGTATGGTTGACCAAAGATAAATTCAAGGTCAATGAAACTTATGACTGCTGGTACACGAATGGCATTTCCAAAGTTAGCTTATATCATGATGGTTTCTTCAGTTGCCAAGCTAAAGATCCGTCTATATTTGAGATGATTAGAAGATATCGCATCCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

257

Amino Acids

29.55

Weight (kDa)

9.73

Isoelectric Point (pI)

41.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012472)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G19140
fragaria_vesca FvH4_5g23960
malus_domestica MD00G1001800.v1.1 MD06G1015300.v1.1
prunus_persica Prupe.5G020400_v2.0.a1
pyrus_communis pycom16g25480
rosa_chinensis RchiOBHm_Chr7g0213391
rosa_laevigata RLG00000002809
rosa_multiflora Rmu_co8237685.1_g000001 Rmu_sc0029136.1_g000001 Rmu_ssc0000262.1_g000033
rosa_roxburghii Rroxscaffold_3G00246030
rosa_rugosa Rorug07G0142100
rosa_samantha Rh7AG273700 Rh7BG269000 Rh7BG269100 Rh7CG292800 Rh7DG281100
rosa_wichuraiana Rw7G023730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 432
AclWI GGATC 2 cut(s) 377, 722
AcoI YGGCCR 1 cut(s) 225
AcsI RAATTY 3 cut(s) 61, 156, 632
AcuI CTGAAG 1 cut(s) 694
AfaI GTAC 2 cut(s) 180, 665
AfiI CCNNNNNNNGG 6 cut(s) 94, 95, 109, 130, 461, 605
AflII CTTAAG 1 cut(s) 392
AgsI TTSAA 6 cut(s) 30, 333, 427, 461, 504, 637
AjuI GAANNNNNNNTTGG 2 cut(s) 617, 649
AloI GAACNNNNNNTCC 2 cut(s) 104, 136
AluBI AGCT 6 cut(s) 186, 342, 415, 550, 690, 722
AluI AGCT 6 cut(s) 186, 342, 415, 550, 690, 722
Alw21I GWGCWC 1 cut(s) 344
Alw26I GTCTC 4 cut(s) 211, 252, 260, 379
AlwI GGATC 2 cut(s) 377, 722
AoxI GGCC 2 cut(s) 225, 576
ApeKI GCWGC 1 cut(s) 219
ApoI RAATTY 3 cut(s) 61, 156, 632
Asp700I GAANNNNTTC 1 cut(s) 25
AspS9I GGNCC 2 cut(s) 133, 577
AsuHPI GGTGA 1 cut(s) 99
AvaII GGWCC 1 cut(s) 133
BaeGI GKGCMC 1 cut(s) 89
BalI TGGCCA 1 cut(s) 227
BanII GRGCYC 1 cut(s) 344
Bbv12I GWGCWC 1 cut(s) 344
BbvCI CCTCAGC 1 cut(s) 561
BbvI GCAGC 1 cut(s) 231
BccI CCATC 1 cut(s) 695
BceAI ACGGC 2 cut(s) 296, 465
BcoDI GTCTC 4 cut(s) 211, 252, 260, 379
BfrI CTTAAG 1 cut(s) 392
BisI GCNGC 1 cut(s) 220
BlsI GCNGC 1 cut(s) 221
Bme18I GGWCC 1 cut(s) 133
BmgT120I GGNCC 2 cut(s) 133, 577
BmiI GGNNCC 2 cut(s) 122, 567
BmrI ACTGGG 2 cut(s) 103, 505
BmsI GCATC 1 cut(s) 467
BmuI ACTGGG 2 cut(s) 103, 505
BplI GAGNNNNNCTC 2 cut(s) 191, 223
Bpu10I CCTNAGC 1 cut(s) 561
BpuEI CTTGAG 1 cut(s) 361
Bsa29I ATCGAT 1 cut(s) 529
BsaI GGTCTC 3 cut(s) 211, 252, 260
BsaWI WCCGGW 3 cut(s) 109, 123, 240
Bsc4I CCNNNNNNNGG 6 cut(s) 94, 95, 109, 130, 461, 605
Bse1I ACTGG 2 cut(s) 98, 500
BseCI ATCGAT 1 cut(s) 529
BseGI GGATG 1 cut(s) 762
BseLI CCNNNNNNNGG 6 cut(s) 94, 95, 109, 130, 461, 605
BseMII CTCAG 4 cut(s) 189, 374, 552, 560
BseNI ACTGG 2 cut(s) 98, 500
BseRI GAGGAG 1 cut(s) 215
BseSI GKGCMC 1 cut(s) 89
BseXI GCAGC 1 cut(s) 231
BsgI GTGCAG 1 cut(s) 385
BshFI GGCC 2 cut(s) 227, 578
BshVI ATCGAT 1 cut(s) 529
BsiHKAI GWGCWC 1 cut(s) 344
BsiSI CCGG 3 cut(s) 110, 124, 241
BslI CCNNNNNNNGG 6 cut(s) 94, 95, 109, 130, 461, 605
BsmAI GTCTC 4 cut(s) 211, 252, 260, 379
BsmBI CGTCTC 1 cut(s) 379
BsmI GAATGC 1 cut(s) 7
BsnI GGCC 2 cut(s) 227, 578
Bso31I GGTCTC 3 cut(s) 211, 252, 260
Bsp1286I GDGCHC 2 cut(s) 89, 344
Bsp143I GATC 5 cut(s) 369, 388, 526, 530, 727
BspANI GGCC 2 cut(s) 227, 578
BspCNI CTCAG 4 cut(s) 190, 373, 553, 559
BspDI ATCGAT 1 cut(s) 529
BspHI TCATGA 1 cut(s) 697
BspLI GGNNCC 2 cut(s) 122, 567
BspPI GGATC 2 cut(s) 377, 722
BspTI CTTAAG 1 cut(s) 392
BspTNI GGTCTC 3 cut(s) 211, 252, 260
BsrI ACTGG 2 cut(s) 98, 500
BssMI GATC 5 cut(s) 369, 388, 526, 530, 727
Bst4CI ACNGT 1 cut(s) 423
Bst6I CTCTTC 1 cut(s) 73
BstAFI CTTAAG 1 cut(s) 392
BstC8I GCNNGC 1 cut(s) 552
BstDEI CTNAG 4 cut(s) 198, 360, 546, 561
BstENI CCTNNNNNAGG 1 cut(s) 459
BstF5I GGATG 1 cut(s) 762
BstKTI GATC 5 cut(s) 372, 391, 529, 533, 730
BstMAI GTCTC 4 cut(s) 211, 252, 260, 379
BstMBI GATC 5 cut(s) 369, 388, 526, 530, 727
BstMWI GCNNNNNNNGC 1 cut(s) 308
BstNSI RCATGY 1 cut(s) 446
BstSLI GKGCMC 1 cut(s) 89
BstV1I GCAGC 1 cut(s) 231
BstX2I RGATCY 1 cut(s) 727
BstYI RGATCY 1 cut(s) 727
Bsu15I ATCGAT 1 cut(s) 529
BsuRI GGCC 2 cut(s) 227, 578
BsuTUI ATCGAT 1 cut(s) 529
BtsCI GGATG 1 cut(s) 762
BtsI GCAGTG 1 cut(s) 388
BtsIMutI CAGTG 4 cut(s) 89, 357, 368, 388
Cac8I GCNNGC 1 cut(s) 552
CciI TCATGA 1 cut(s) 697
Cfr13I GGNCC 2 cut(s) 133, 577
ClaI ATCGAT 1 cut(s) 529
Csp6I GTAC 2 cut(s) 179, 664
CviAII CATG 2 cut(s) 443, 698
CviQI GTAC 2 cut(s) 179, 664
DdeI CTNAG 4 cut(s) 198, 360, 546, 561
DpnI GATC 5 cut(s) 371, 390, 528, 532, 729
DpnII GATC 5 cut(s) 369, 388, 526, 530, 727
EaeI YGGCCR 1 cut(s) 225
Eam1104I CTCTTC 1 cut(s) 73
EarI CTCTTC 1 cut(s) 73
Ecl136II GAGCTC 1 cut(s) 342
Eco24I GRGCYC 1 cut(s) 344
Eco31I GGTCTC 3 cut(s) 211, 252, 260
Eco32I GATATC 1 cut(s) 758
Eco47I GGWCC 1 cut(s) 133
Eco53kI GAGCTC 1 cut(s) 342
Eco57I CTGAAG 1 cut(s) 694
EcoICRI GAGCTC 1 cut(s) 342
EcoNI CCTNNNNNAGG 1 cut(s) 459
EcoO109I RGGNCCY 1 cut(s) 577
EcoRI GAATTC 1 cut(s) 156
EcoRV GATATC 1 cut(s) 758
EcoT38I GRGCYC 1 cut(s) 344
Esp3I CGTCTC 1 cut(s) 379
FaeI CATG 2 cut(s) 446, 701
FaiI YATR 9 cut(s) 18, 432, 444, 486, 616, 654, 694, 699, 737
FatI CATG 2 cut(s) 442, 697
Fnu4HI GCNGC 1 cut(s) 220
FokI GGATG 1 cut(s) 749
FriOI GRGCYC 1 cut(s) 344
Fsp4HI GCNGC 1 cut(s) 220
GluI GCNGC 1 cut(s) 220
HaeIII GGCC 2 cut(s) 227, 578
HapII CCGG 3 cut(s) 110, 124, 241
Hin1II CATG 2 cut(s) 446, 701
HincII GTYRAC 1 cut(s) 621
HindII GTYRAC 1 cut(s) 621
HindIII AAGCTT 1 cut(s) 184
HinfI GANTC 2 cut(s) 194, 544
HpaII CCGG 3 cut(s) 110, 124, 241
HphI GGTGA 1 cut(s) 99
Hpy166II GTNNAC 2 cut(s) 621, 666
Hpy188I TCNGA 3 cut(s) 199, 339, 543
Hpy188III TCNNGA 1 cut(s) 698
Hpy8I GTNNAC 2 cut(s) 621, 666
Hpy99I CGWCG 2 cut(s) 311, 323
HpyAV CCTTC 5 cut(s) 208, 269, 313, 462, 590
HpyCH4III ACNGT 1 cut(s) 423
HpyCH4V TGCA 4 cut(s) 142, 366, 554, 594
HpyF10VI GCNNNNNNNGC 1 cut(s) 308
HpyF3I CTNAG 4 cut(s) 198, 360, 546, 561
Hsp92II CATG 2 cut(s) 446, 701
Kzo9I GATC 5 cut(s) 369, 388, 526, 530, 727
LmnI GCTCC 1 cut(s) 571
Lsp1109I GCAGC 1 cut(s) 231
LweI GCATC 1 cut(s) 467
MalI GATC 5 cut(s) 371, 390, 528, 532, 729
MboI GATC 5 cut(s) 369, 388, 526, 530, 727
MboII GAAGA 7 cut(s) 60, 63, 321, 477, 525, 700, 765
MflI RGATCY 1 cut(s) 727
MhlI GDGCHC 2 cut(s) 89, 344
MlsI TGGCCA 1 cut(s) 227
MluNI TGGCCA 1 cut(s) 227
MlyI GAGTC 1 cut(s) 538
MnlI CCTC 7 cut(s) 76, 193, 196, 367, 556, 568, 594
Mox20I TGGCCA 1 cut(s) 227
MroXI GAANNNNTTC 1 cut(s) 25
MscI TGGCCA 1 cut(s) 227
MseI TTAA 6 cut(s) 165, 188, 233, 393, 510, 588
Msp20I TGGCCA 1 cut(s) 227
MspCI CTTAAG 1 cut(s) 392
MspI CCGG 3 cut(s) 110, 124, 241
Mva1269I GAATGC 1 cut(s) 7
MwoI GCNNNNNNNGC 1 cut(s) 308
NdeII GATC 5 cut(s) 369, 388, 526, 530, 727
NlaIII CATG 2 cut(s) 446, 701
NlaIV GGNNCC 2 cut(s) 122, 567
NspI RCATGY 1 cut(s) 446
PagI TCATGA 1 cut(s) 697
PcsI WCGNNNNNNNCGW 1 cut(s) 315
PctI GAATGC 1 cut(s) 7
PdmI GAANNNNTTC 1 cut(s) 25
PfeI GAWTC 1 cut(s) 194
PkrI GCNGC 1 cut(s) 221
PleI GAGTC 1 cut(s) 538
PpsI GAGTC 1 cut(s) 538
PsiI TTATAA 1 cut(s) 432
Psp124BI GAGCTC 1 cut(s) 344
PspN4I GGNNCC 2 cut(s) 122, 567
PspPI GGNCC 2 cut(s) 133, 577
PsuI RGATCY 1 cut(s) 727
RsaI GTAC 2 cut(s) 180, 665
RsaNI GTAC 2 cut(s) 179, 664
SacI GAGCTC 1 cut(s) 344
SaqAI TTAA 6 cut(s) 165, 188, 233, 393, 510, 588
SatI GCNGC 1 cut(s) 220
Sau3AI GATC 5 cut(s) 369, 388, 526, 530, 727
Sau96I GGNCC 2 cut(s) 133, 577
SchI GAGTC 1 cut(s) 538
SduI GDGCHC 2 cut(s) 89, 344
SfaNI GCATC 1 cut(s) 467
SinI GGWCC 1 cut(s) 133
SmlI CTYRAG 2 cut(s) 376, 392
SmoI CTYRAG 2 cut(s) 376, 392
SstI GAGCTC 1 cut(s) 344
TaaI ACNGT 1 cut(s) 423
TaqI TCGA 2 cut(s) 475, 529
TfiI GAWTC 1 cut(s) 194
Tru1I TTAA 6 cut(s) 165, 188, 233, 393, 510, 588
Tru9I TTAA 6 cut(s) 165, 188, 233, 393, 510, 588
TscAI CASTG 4 cut(s) 89, 357, 368, 388
TseI GCWGC 1 cut(s) 219
TspDTI ATGAA 2 cut(s) 17, 660
TspGWI ACGGA 1 cut(s) 720
TspRI CASTG 4 cut(s) 89, 357, 368, 388
Vha464I CTTAAG 1 cut(s) 392
VpaK11BI GGWCC 1 cut(s) 133
XagI CCTNNNNNAGG 1 cut(s) 459
XapI RAATTY 3 cut(s) 61, 156, 632
XceI RCATGY 1 cut(s) 446
XmnI GAANNNNTTC 1 cut(s) 25
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.