pycom16g26190

DNA-binding protein ESCAROLA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0006163
Physical Location & Seq
Reverse (-)
28465151 .. 28468692
3542 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1128 bp
ATGTCAGGATCTGAGACGGGAGTGATGACGAGTAGGGAGCCGTTTCTGCAGAAGAGTCCTATTCAGTCACAGTCCGCCATCCAGAGTCTGCGGTTGAACTTCAGTGCTGACGGCGGCCCCGCTCTCTACAAGCCTGTTGCCACCGCCACCTCACCTGCTTACCAGCCCTCGGTCACCTCCGCCGCCGCTTCCGGCGGAGGTGCTGTTGTCCAGGGGGCTGCCGGAGAGGGTGCAGTTATGGCCCCTGCTGCTACTGCTGGGCTTAACATGAACATGGGTACTGAGCCAATGAAGAAGAAGAGAGGGAGGCCCAGGAAGTACGGGCCAGATGGCACAATGGCATTGGCTCTATCACCATCAGCCCCGCCTGTGACCGTTACCTCGTCCAGTGTCGGAGCCTTTTCTCCTCCTCCTGCTCCTGCTCCTCCTGCTCCTCCTTCTGGGGGAGGTTCTGCCTCACCACCACCCACTTCCACTTCCACCAAGAAATCAAGAGGCAGACCACCTGGTTCTTCCAAGAAGCAACAATTGGATGCTTTGGGAGCTCCGGGATTTGGATTTACACCACATGTTATCACTGTAAAAGCTGGAGAGGATGTATGGTCAAAAATAATGTCATTCTCTCAGAATGGTCCTAGAGCTGTTTGCATATTGTCTGCAACTGGAGCCATCTCTAATGTGACTCTTCGCCAACCGGCCACATCTGGTGGAACTGTAACATATGAGGGGAGGTTTGAAATTCTGTCACTCTCAGGCTCGTTTCTTCTATCTGAAATTGGTGGTCAGCGGAGTAGAACTGGGGGCTTAAGTGTGTCATTATCTGGCCCAGATGGCCGAGTTTTAGGTGGTGGCGTGGCAGGTCTTCTAACGGCTGCCTGCCCTGTTCAGGTGGTAGTCGGAAGTTTTGTTGCAGATGGTCGGAAAGAATCAAAGACTGCGAACCAAATGGACCCGTTGTCCGTAGCACCAAAATTTGATCCGGGCAGTGGTCCAACAGGAGCAAGTAGCCCCCAGTCACGTGGAACTCTGAGTGAATCCTCAGGCGGGCCTGGAAGTCCGCTTAACCAAAGCACAGGAGCCTGCAATAACAATAACCTGCAAGGCATGTCAAGCATGCCATGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

376

Amino Acids

37.34

Weight (kDa)

9.93

Isoelectric Point (pI)

62.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 188 - 303 2.7e-27 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 163
Acc36I ACCTGC 3 cut(s) 163, 848, 1104
AccBSI CCGCTC 1 cut(s) 122
AclWI GGATC 2 cut(s) 16, 971
AcoI YGGCCR 2 cut(s) 696, 832
AcsI RAATTY 2 cut(s) 738, 971
AcuI CTGAAG 1 cut(s) 85
AcvI CACGTG 1 cut(s) 1019
AfaI GTAC 2 cut(s) 280, 320
AfiI CCNNNNNNNGG 7 cut(s) 169, 440, 443, 554, 886, 986, 1044
AflII CTTAAG 1 cut(s) 805
AflIII ACRYGT 1 cut(s) 568
AgsI TTSAA 2 cut(s) 97, 737
AhdI GACNNNNNGTC 1 cut(s) 955
AjnI CCWGG 4 cut(s) 210, 311, 505, 1048
AjuI GAANNNNNNNTTGG 2 cut(s) 512, 544
AluBI AGCT 3 cut(s) 545, 587, 641
AluI AGCT 3 cut(s) 545, 587, 641
Alw21I GWGCWC 1 cut(s) 547
Alw26I GTCTC 1 cut(s) 8
AlwI GGATC 2 cut(s) 16, 971
AlwNI CAGNNNCTG 2 cut(s) 11, 88
AoxI GGCC 8 cut(s) 115, 240, 308, 323, 696, 823, 832, 1046
ApeKI GCWGC 3 cut(s) 218, 248, 872
ApoI RAATTY 2 cut(s) 738, 971
ArsI GACNNNNNNTTYG 2 cut(s) 599, 631
AspS9I GGNCC 9 cut(s) 116, 241, 309, 323, 632, 824, 949, 989, 1046
AsuC2I CCSGG 2 cut(s) 549, 981
AsuHPI GGTGA 4 cut(s) 144, 166, 345, 450
AvaII GGWCC 3 cut(s) 632, 949, 989
AxyI CCTNAGG 1 cut(s) 1039
BanII GRGCYC 1 cut(s) 547
BbrPI CACGTG 1 cut(s) 1019
BbsI GAAGAC 1 cut(s) 854
Bbv12I GWGCWC 1 cut(s) 547
BbvI GCAGC 3 cut(s) 205, 235, 859
BccI CCATC 6 cut(s) 86, 323, 364, 677, 824, 908
BceAI ACGGC 3 cut(s) 25, 127, 885
BciT130I CCWGG 4 cut(s) 212, 313, 507, 1050
BcnI CCSGG 2 cut(s) 549, 981
BcoDI GTCTC 1 cut(s) 8
BfaI CTAG 1 cut(s) 636
BfmI CTRYAG 1 cut(s) 47
BfrI CTTAAG 1 cut(s) 805
BfuAI ACCTGC 3 cut(s) 163, 848, 1104
BglI GCCNNNNNGGC 1 cut(s) 831
BisI GCNGC 6 cut(s) 115, 183, 186, 219, 249, 873
BlsI GCNGC 6 cut(s) 116, 184, 187, 220, 250, 874
Bme1390I CCNGG 6 cut(s) 212, 313, 507, 549, 981, 1050
Bme18I GGWCC 3 cut(s) 632, 949, 989
BmeRI GACNNNNNGTC 1 cut(s) 955
BmgT120I GGNCC 9 cut(s) 116, 241, 309, 323, 632, 824, 949, 989, 1046
BmiI GGNNCC 7 cut(s) 39, 118, 243, 397, 667, 951, 1078
BmrFI CCNGG 6 cut(s) 212, 313, 507, 549, 981, 1050
BmrI ACTGGG 2 cut(s) 807, 1006
BmsI GCATC 1 cut(s) 523
BmuI ACTGGG 2 cut(s) 807, 1006
BpiI GAAGAC 1 cut(s) 854
BpmI CTGGAG 2 cut(s) 609, 684
BpuMI CCSGG 2 cut(s) 549, 981
BsaAI YACGTR 1 cut(s) 1019
BsaJI CCNNGG 4 cut(s) 168, 211, 311, 1118
BsaXI ACNNNNNCTCC 4 cut(s) 189, 219, 582, 612
Bsc4I CCNNNNNNNGG 7 cut(s) 169, 440, 443, 554, 886, 986, 1044
Bse118I RCCGGY 1 cut(s) 694
Bse1I ACTGG 4 cut(s) 387, 667, 802, 1012
Bse21I CCTNAGG 1 cut(s) 1039
BseBI CCWGG 4 cut(s) 212, 313, 507, 1050
BseDI CCNNGG 4 cut(s) 168, 211, 311, 1118
BseGI GGATG 3 cut(s) 78, 538, 601
BseLI CCNNNNNNNGG 7 cut(s) 169, 440, 443, 554, 886, 986, 1044
BseMII CTCAG 5 cut(s) 273, 638, 765, 1019, 1053
BseNI ACTGG 4 cut(s) 387, 667, 802, 1012
BseRI GAGGAG 4 cut(s) 396, 399, 414, 423
BseXI GCAGC 3 cut(s) 205, 235, 859
BseYI CCCAGC 1 cut(s) 257
BsgI GTGCAG 1 cut(s) 252
BshFI GGCC 8 cut(s) 117, 242, 310, 325, 698, 825, 834, 1048
BsiHKAI GWGCWC 1 cut(s) 547
BsiSI CCGG 5 cut(s) 192, 222, 548, 695, 980
BslI CCNNNNNNNGG 7 cut(s) 169, 440, 443, 554, 886, 986, 1044
BsmAI GTCTC 1 cut(s) 8
BsmBI CGTCTC 1 cut(s) 8
BsnI GGCC 8 cut(s) 117, 242, 310, 325, 698, 825, 834, 1048
Bsp1286I GDGCHC 1 cut(s) 547
Bsp143I GATC 2 cut(s) 8, 976
Bsp19I CCATGG 1 cut(s) 1118
BspANI GGCC 8 cut(s) 117, 242, 310, 325, 698, 825, 834, 1048
BspCNI CTCAG 6 cut(s) 4, 274, 637, 764, 1020, 1052
BspLI GGNNCC 7 cut(s) 39, 118, 243, 397, 667, 951, 1078
BspMAI CTGCAG 1 cut(s) 51
BspMI ACCTGC 3 cut(s) 163, 848, 1104
BspPI GGATC 2 cut(s) 16, 971
BspTI CTTAAG 1 cut(s) 805
BsrBI CCGCTC 1 cut(s) 122
BsrFI RCCGGY 1 cut(s) 694
BsrI ACTGG 4 cut(s) 387, 667, 802, 1012
BssAI RCCGGY 1 cut(s) 694
BssECI CCNNGG 4 cut(s) 168, 211, 311, 1118
BssMI GATC 2 cut(s) 8, 976
BssT1I CCWWGG 1 cut(s) 1118
Bst2UI CCWGG 4 cut(s) 212, 313, 507, 1050
Bst4CI ACNGT 4 cut(s) 72, 376, 580, 715
Bst6I CTCTTC 3 cut(s) 47, 293, 690
BstAFI CTTAAG 1 cut(s) 805
BstBAI YACGTR 1 cut(s) 1019
BstC8I GCNNGC 4 cut(s) 877, 1046, 1081, 1115
BstDEI CTNAG 6 cut(s) 12, 282, 624, 751, 1028, 1039
BstDSI CCRYGG 1 cut(s) 1118
BstEII GGTNACC 1 cut(s) 172
BstF5I GGATG 3 cut(s) 78, 538, 601
BstKTI GATC 2 cut(s) 11, 979
BstMAI GTCTC 1 cut(s) 8
BstMBI GATC 2 cut(s) 8, 976
BstMWI GCNNNNNNNGC 9 cut(s) 46, 239, 248, 254, 428, 542, 665, 831, 1110
BstNI CCWGG 4 cut(s) 212, 313, 507, 1050
BstNSI RCATGY 3 cut(s) 572, 1108, 1117
BstPI GGTNACC 1 cut(s) 172
BstSCI CCNGG 6 cut(s) 210, 311, 505, 547, 979, 1048
BstSFI CTRYAG 1 cut(s) 47
BstV1I GCAGC 3 cut(s) 205, 235, 859
BstV2I GAAGAC 1 cut(s) 854
BstX2I RGATCY 1 cut(s) 8
BstXI CCANNNNNNTGG 1 cut(s) 1019
BstYI RGATCY 1 cut(s) 8
Bsu36I CCTNAGG 1 cut(s) 1039
BsuRI GGCC 8 cut(s) 117, 242, 310, 325, 698, 825, 834, 1048
BtgI CCRYGG 1 cut(s) 1118
BtsCI GGATG 3 cut(s) 78, 538, 601
BtsI GCAGTG 1 cut(s) 991
BtsIMutI CAGTG 4 cut(s) 109, 394, 576, 991
BveI ACCTGC 3 cut(s) 163, 848, 1104
Cac8I GCNNGC 4 cut(s) 877, 1046, 1081, 1115
CaiI CAGNNNCTG 2 cut(s) 11, 88
Cfr10I RCCGGY 1 cut(s) 694
Cfr13I GGNCC 9 cut(s) 116, 241, 309, 323, 632, 824, 949, 989, 1046
CsiI ACCWGGT 1 cut(s) 505
Csp6I GTAC 2 cut(s) 279, 319
CviAII CATG 6 cut(s) 268, 274, 569, 1105, 1114, 1119
CviQI GTAC 2 cut(s) 279, 319
DdeI CTNAG 6 cut(s) 12, 282, 624, 751, 1028, 1039
DpnI GATC 2 cut(s) 10, 978
DpnII GATC 2 cut(s) 8, 976
DriI GACNNNNNGTC 1 cut(s) 955
EaeI YGGCCR 2 cut(s) 696, 832
Eam1104I CTCTTC 3 cut(s) 47, 293, 690
Eam1105I GACNNNNNGTC 1 cut(s) 955
EarI CTCTTC 3 cut(s) 47, 293, 690
EciI GGCGGA 3 cut(s) 64, 169, 210
Ecl136II GAGCTC 1 cut(s) 545
Eco130I CCWWGG 1 cut(s) 1118
Eco24I GRGCYC 1 cut(s) 547
Eco47I GGWCC 3 cut(s) 632, 949, 989
Eco53kI GAGCTC 1 cut(s) 545
Eco57I CTGAAG 1 cut(s) 85
Eco72I CACGTG 1 cut(s) 1019
Eco81I CCTNAGG 1 cut(s) 1039
Eco91I GGTNACC 1 cut(s) 172
EcoICRI GAGCTC 1 cut(s) 545
EcoO65I GGTNACC 1 cut(s) 172
EcoRII CCWGG 4 cut(s) 210, 311, 505, 1048
EcoT14I CCWWGG 1 cut(s) 1118
EcoT38I GRGCYC 1 cut(s) 547
ErhI CCWWGG 1 cut(s) 1118
Esp3I CGTCTC 1 cut(s) 8
FaeI CATG 6 cut(s) 271, 277, 572, 1108, 1117, 1122
FatI CATG 6 cut(s) 267, 273, 568, 1104, 1113, 1118
FauI CCCGC 3 cut(s) 127, 372, 1037
FauNDI CATATG 1 cut(s) 721
Fnu4HI GCNGC 6 cut(s) 115, 183, 186, 219, 249, 873
FokI GGATG 3 cut(s) 65, 545, 608
FriOI GRGCYC 1 cut(s) 547
Fsp4HI GCNGC 6 cut(s) 115, 183, 186, 219, 249, 873
FspBI CTAG 1 cut(s) 636
GluI GCNGC 6 cut(s) 115, 183, 186, 219, 249, 873
GsaI CCCAGC 1 cut(s) 261
GsuI CTGGAG 2 cut(s) 609, 684
HaeIII GGCC 8 cut(s) 117, 242, 310, 325, 698, 825, 834, 1048
HapII CCGG 5 cut(s) 192, 222, 548, 695, 980
Hin1II CATG 6 cut(s) 271, 277, 572, 1108, 1117, 1122
HinfI GANTC 5 cut(s) 55, 85, 682, 926, 1034
HpaII CCGG 5 cut(s) 192, 222, 548, 695, 980
HphI GGTGA 4 cut(s) 144, 166, 345, 450
Hpy188I TCNGA 7 cut(s) 13, 395, 627, 772, 899, 921, 1029
Hpy188III TCNNGA 3 cut(s) 6, 82, 492
HpyAV CCTTC 1 cut(s) 447
HpyCH4III ACNGT 4 cut(s) 72, 376, 580, 715
HpyCH4IV ACGT 1 cut(s) 1018
HpyCH4V TGCA 7 cut(s) 49, 233, 648, 659, 911, 1083, 1099
HpyF10VI GCNNNNNNNGC 9 cut(s) 46, 239, 248, 254, 428, 542, 665, 831, 1110
HpyF3I CTNAG 6 cut(s) 12, 282, 624, 751, 1028, 1039
HpySE526I ACGT 1 cut(s) 1018
Hsp92II CATG 6 cut(s) 271, 277, 572, 1108, 1117, 1122
Kzo9I GATC 2 cut(s) 8, 976
Lsp1109I GCAGC 3 cut(s) 205, 235, 859
LweI GCATC 1 cut(s) 523
MabI ACCWGGT 1 cut(s) 505
MaeI CTAG 1 cut(s) 636
MaeII ACGT 1 cut(s) 1018
MaeIII GTNAC 8 cut(s) 66, 172, 370, 376, 679, 715, 744, 1014
MalI GATC 2 cut(s) 10, 978
MbiI CCGCTC 1 cut(s) 122
MboI GATC 2 cut(s) 8, 976
MboII GAAGA 8 cut(s) 64, 304, 307, 310, 504, 677, 755, 854
MfeI CAATTG 1 cut(s) 527
MflI RGATCY 1 cut(s) 8
MhlI GDGCHC 1 cut(s) 547
MluCI AATT 4 cut(s) 527, 738, 774, 971
MlyI GAGTC 3 cut(s) 64, 94, 676
MmeI TCCRAC 4 cut(s) 373, 877, 899, 1016
MseI TTAA 3 cut(s) 264, 806, 1062
MslI CAYNNNNRTG 2 cut(s) 272, 1123
MspA1I CMGCKG 1 cut(s) 787
MspCI CTTAAG 1 cut(s) 805
MspI CCGG 5 cut(s) 192, 222, 548, 695, 980
MspR9I CCNGG 6 cut(s) 212, 313, 507, 549, 981, 1050
MunI CAATTG 1 cut(s) 527
MvaI CCWGG 4 cut(s) 212, 313, 507, 1050
MwoI GCNNNNNNNGC 9 cut(s) 46, 239, 248, 254, 428, 542, 665, 831, 1110
NciI CCSGG 2 cut(s) 549, 981
NcoI CCATGG 1 cut(s) 1118
NdeI CATATG 1 cut(s) 721
NdeII GATC 2 cut(s) 8, 976
NlaIII CATG 6 cut(s) 271, 277, 572, 1108, 1117, 1122
NlaIV GGNNCC 7 cut(s) 39, 118, 243, 397, 667, 951, 1078
NmeAIII GCCGAG 1 cut(s) 860
NmuCI GTSAC 6 cut(s) 66, 172, 370, 679, 744, 1014
NspI RCATGY 3 cut(s) 572, 1108, 1117
PaeI GCATGC 1 cut(s) 1117
PaqCI CACCTGC 1 cut(s) 163
PciI ACATGT 1 cut(s) 568
PfeI GAWTC 2 cut(s) 926, 1034
PfoI TCCNGGA 1 cut(s) 547
PkrI GCNGC 6 cut(s) 116, 184, 187, 220, 250, 874
PleI GAGTC 3 cut(s) 63, 93, 676
PmaCI CACGTG 1 cut(s) 1019
PmlI CACGTG 1 cut(s) 1019
PpsI GAGTC 3 cut(s) 63, 93, 676
Ppu21I YACGTR 1 cut(s) 1019
PscI ACATGT 1 cut(s) 568
Psp124BI GAGCTC 1 cut(s) 547
Psp6I CCWGG 4 cut(s) 210, 311, 505, 1048
PspCI CACGTG 1 cut(s) 1019
PspEI GGTNACC 1 cut(s) 172
PspFI CCCAGC 1 cut(s) 257
PspGI CCWGG 4 cut(s) 210, 311, 505, 1048
PspN4I GGNNCC 7 cut(s) 39, 118, 243, 397, 667, 951, 1078
PspPI GGNCC 9 cut(s) 116, 241, 309, 323, 632, 824, 949, 989, 1046
PstI CTGCAG 1 cut(s) 51
PstNI CAGNNNCTG 2 cut(s) 11, 88
PsuI RGATCY 1 cut(s) 8
RsaI GTAC 2 cut(s) 280, 320
RsaNI GTAC 2 cut(s) 279, 319
RseI CAYNNNNRTG 2 cut(s) 272, 1123
SacI GAGCTC 1 cut(s) 547
SaqAI TTAA 3 cut(s) 264, 806, 1062
SatI GCNGC 6 cut(s) 115, 183, 186, 219, 249, 873
Sau3AI GATC 2 cut(s) 8, 976
Sau96I GGNCC 9 cut(s) 116, 241, 309, 323, 632, 824, 949, 989, 1046
SchI GAGTC 3 cut(s) 64, 94, 676
ScrFI CCNGG 6 cut(s) 212, 313, 507, 549, 981, 1050
SduI GDGCHC 1 cut(s) 547
SexAI ACCWGGT 1 cut(s) 505
SfaNI GCATC 1 cut(s) 523
SfcI CTRYAG 1 cut(s) 47
SfiI GGCCNNNNNGGCC 1 cut(s) 831
SinI GGWCC 3 cut(s) 632, 949, 989
SmiMI CAYNNNNRTG 2 cut(s) 272, 1123
SmlI CTYRAG 1 cut(s) 805
SmoI CTYRAG 1 cut(s) 805
SphI GCATGC 1 cut(s) 1117
Sse9I AATT 4 cut(s) 527, 738, 774, 971
SspMI CTAG 1 cut(s) 636
SstI GAGCTC 1 cut(s) 547
StyD4I CCNGG 6 cut(s) 210, 311, 505, 547, 979, 1048
StyI CCWWGG 1 cut(s) 1118
TaaI ACNGT 4 cut(s) 72, 376, 580, 715
TaiI ACGT 1 cut(s) 1021
TaqII GACCGA 1 cut(s) 160
TasI AATT 4 cut(s) 527, 738, 774, 971
TauI GCSGC 3 cut(s) 117, 185, 188
TfiI GAWTC 2 cut(s) 926, 1034
Tru1I TTAA 3 cut(s) 264, 806, 1062
Tru9I TTAA 3 cut(s) 264, 806, 1062
TscAI CASTG 4 cut(s) 109, 394, 583, 991
TseFI GTSAC 6 cut(s) 66, 172, 370, 679, 744, 1014
TseI GCWGC 3 cut(s) 218, 248, 872
Tsp45I GTSAC 6 cut(s) 66, 172, 370, 679, 744, 1014
TspDTI ATGAA 2 cut(s) 284, 305
TspGWI ACGGA 1 cut(s) 949
TspRI CASTG 4 cut(s) 109, 394, 583, 991
Vha464I CTTAAG 1 cut(s) 805
VpaK11BI GGWCC 3 cut(s) 632, 949, 989
XapI RAATTY 2 cut(s) 738, 971
XceI RCATGY 3 cut(s) 572, 1108, 1117
XspI CTAG 1 cut(s) 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.