pycom17g00430

BRO1-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0003879
Physical Location & Seq
Forward (+)
307607 .. 309958
2352 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1152 bp
ATGGGGTGCGCCTCTTCTGTTTATGCTATTCGAGGAAAGAAGAAGTTGACTGTCCCTGAAGTCGTGGTCTTTGTCCCGTCTACGCGAATCCCGGTTCAGTCTGATGTTCAAAAGCGTCTTAGAGGCCTCATTCCAAAAGATCTTGTTGACAGACTGTCATCGCTTCGCAATCAGATTGTCTTAGTTGCAGAGGATACTGGTGGATCTGCTATATCTGAACTGCGGCGTGCGCTGGAGGAGTACTTGTCTGTTTTAATTGGGCTCACTAAGAAAGAATATGGTCTGGAGAGGTTGGTTGAATTCAAGTGGAAATGTTTGGAGGATGGAAAACAGGAAGCATGTGTGGCAAACTCCTGGTTTGAAGTACTCTCAGTTCTGCACCTGATGGCTATGCTTACATTGTCGGAGGTTGATACCTTGATGATCCCACTCGACCATTCTGGTTCGACTATAAGGACAGTTTCTTCAGATTCCAAGAGAGAAGCTGTCGACTTATTGCTCAAGGCATCCAGCTACTTGGAATTCTGTGTCCGGGATGTCCTGGTTCGCATTCCACCCGAAATCAAGAAAAATTTGCCCAAAATTTTGCGTGATGGTGTGTTGGAGGCCATTTCTTATCAAACTCTAGGCCAGGGAACAGAAATCCAGCTTGGCTTAGCTGTAGAATGTCAGAAGGCCACATTGTCAGTTAAAAGGAGATTGGCATGTGAACAGTTGGCCTATTTTAGTCAGGCCTATCACTGCTTGTCAGGATGCGACACAAATAATGGGTGCGGGAAGAAGCATATCTGGTTCATCAAATGGAAATTTCTTGAGTCCAAGGCTGCAGCATACTACTACCACGGCCTGATCCTTGACAAGGGCAATGAACCGTCATGCCACGTTAGCGCTGTGTGCTGTTTTCTTGCAGCGGAAGAACTTCTATCAGAGAGCAAGAAGGCTTGCTTAACCTTTTGCCTTGCAGCTCCAGTTACCAGGGTTCTACAAGCATTGCCTGACCTACCAGAATTTCAACTGTCATTAAGACCTGATGACTATCAGATACCTGAAATCGACCCGGCCTGGGATTCCGAAAGTTGGGAAGCTGAGGGCCAGACCTTGAAAGAGCACCTAAAAGATAGCGATGACGACGAGATTGAAACTCCAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BRO1

Protein Analysis

384

Amino Acids

42.81

Weight (kDa)

5.4

Isoelectric Point (pI)

48.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 80, 489
AccII CGCG 1 cut(s) 85
AciI CCGC 3 cut(s) 223, 774, 911
AclWI GGATC 3 cut(s) 211, 418, 844
AcsI RAATTY 6 cut(s) 299, 521, 571, 582, 806, 1007
AcuI CTGAAG 2 cut(s) 78, 450
AfaI GTAC 2 cut(s) 242, 366
AfeI AGCGCT 1 cut(s) 889
AfiI CCNNNNNNNGG 3 cut(s) 859, 1063, 1077
AgsI TTSAA 7 cut(s) 110, 299, 304, 362, 1013, 1102, 1139
AhdI GACNNNNNGTC 1 cut(s) 154
AjnI CCWGG 5 cut(s) 353, 540, 630, 974, 1061
AjuI GAANNNNNNNTTGG 2 cut(s) 633, 665
AluBI AGCT 6 cut(s) 485, 513, 649, 659, 965, 1085
AluI AGCT 6 cut(s) 485, 513, 649, 659, 965, 1085
Alw21I GWGCWC 1 cut(s) 1110
AlwI GGATC 3 cut(s) 211, 418, 844
Aor51HI AGCGCT 1 cut(s) 889
AoxI GGCC 9 cut(s) 124, 606, 628, 675, 717, 732, 844, 1059, 1090
ApeKI GCWGC 4 cut(s) 824, 827, 908, 962
ApoI RAATTY 6 cut(s) 299, 521, 571, 582, 806, 1007
Asp700I GAANNNNTTC 1 cut(s) 918
AspLEI GCGC 3 cut(s) 11, 232, 890
AspS9I GGNCC 1 cut(s) 1090
AsuC2I CCSGG 3 cut(s) 92, 533, 1058
BanII GRGCYC 1 cut(s) 264
Bbv12I GWGCWC 1 cut(s) 1110
BbvCI CCTCAGC 1 cut(s) 1086
BbvI GCAGC 4 cut(s) 811, 839, 920, 974
BccI CCATC 3 cut(s) 317, 379, 587
BceAI ACGGC 1 cut(s) 859
BciT130I CCWGG 5 cut(s) 355, 542, 632, 976, 1063
BciVI GTATCC 1 cut(s) 187
BcnI CCSGG 3 cut(s) 92, 533, 1058
BfaI CTAG 1 cut(s) 626
BfmI CTRYAG 2 cut(s) 660, 825
BfoI RGCGCY 1 cut(s) 891
BfuI GTATCC 1 cut(s) 187
BglII AGATCT 1 cut(s) 139
BisI GCNGC 5 cut(s) 224, 825, 828, 909, 963
BlpI GCTNAGC 1 cut(s) 655
BlsI GCNGC 5 cut(s) 225, 826, 829, 910, 964
BmcAI AGTACT 2 cut(s) 242, 366
Bme1390I CCNGG 8 cut(s) 92, 355, 533, 542, 632, 976, 1058, 1063
BmeRI GACNNNNNGTC 1 cut(s) 154
BmgT120I GGNCC 1 cut(s) 1090
BmrFI CCNGG 8 cut(s) 92, 355, 533, 542, 632, 976, 1058, 1063
BmsI GCATC 2 cut(s) 515, 743
BpmI CTGGAG 4 cut(s) 254, 305, 951, 1128
Bpu10I CCTNAGC 1 cut(s) 1086
Bpu1102I GCTNAGC 1 cut(s) 655
BpuEI CTTGAG 2 cut(s) 485, 833
BpuMI CCSGG 3 cut(s) 92, 533, 1058
BsaBI GATNNNNATC 1 cut(s) 1035
BsaJI CCNNGG 5 cut(s) 631, 819, 841, 975, 1062
BsaXI ACNNNNNCTCC 2 cut(s) 230, 260
Bsc4I CCNNNNNNNGG 3 cut(s) 859, 1063, 1077
Bse1I ACTGG 2 cut(s) 202, 968
Bse3DI GCAATG 2 cut(s) 871, 989
Bse8I GATNNNNATC 1 cut(s) 1035
BseBI CCWGG 5 cut(s) 355, 542, 632, 976, 1063
BseDI CCNNGG 5 cut(s) 631, 819, 841, 975, 1062
BseGI GGATG 4 cut(s) 328, 506, 541, 758
BseJI GATNNNNATC 1 cut(s) 1035
BseLI CCNNNNNNNGG 3 cut(s) 859, 1063, 1077
BseMI GCAATG 2 cut(s) 871, 989
BseMII CTCAG 2 cut(s) 384, 1077
BseNI ACTGG 2 cut(s) 202, 968
BseRI GAGGAG 1 cut(s) 251
BseXI GCAGC 4 cut(s) 811, 839, 920, 974
BsgI GTGCAG 1 cut(s) 362
Bsh1236I CGCG 1 cut(s) 85
BshFI GGCC 9 cut(s) 126, 608, 630, 677, 719, 734, 846, 1061, 1092
BsiHKAI GWGCWC 1 cut(s) 1110
BsiSI CCGG 3 cut(s) 92, 532, 1058
BslFI GGGAC 2 cut(s) 38, 59
BslI CCNNNNNNNGG 3 cut(s) 859, 1063, 1077
BsmFI GGGAC 2 cut(s) 38, 59
BsmI GAATGC 1 cut(s) 549
BsnI GGCC 9 cut(s) 126, 608, 630, 677, 719, 734, 846, 1061, 1092
Bsp1286I GDGCHC 2 cut(s) 264, 1110
Bsp143I GATC 4 cut(s) 139, 203, 423, 849
Bsp1720I GCTNAGC 1 cut(s) 655
BspACI CCGC 3 cut(s) 223, 774, 911
BspANI GGCC 9 cut(s) 126, 608, 630, 677, 719, 734, 846, 1061, 1092
BspCNI CTCAG 2 cut(s) 383, 1078
BspFNI CGCG 1 cut(s) 85
BspMAI CTGCAG 1 cut(s) 829
BspPI GGATC 3 cut(s) 211, 418, 844
BsrDI GCAATG 2 cut(s) 871, 989
BsrI ACTGG 2 cut(s) 202, 968
BssECI CCNNGG 5 cut(s) 631, 819, 841, 975, 1062
BssMI GATC 4 cut(s) 139, 203, 423, 849
BssT1I CCWWGG 1 cut(s) 819
Bst2UI CCWGG 5 cut(s) 355, 542, 632, 976, 1063
Bst4CI ACNGT 6 cut(s) 52, 156, 460, 714, 873, 1017
Bst6I CTCTTC 1 cut(s) 19
BstC8I GCNNGC 2 cut(s) 228, 943
BstDEI CTNAG 6 cut(s) 119, 181, 267, 370, 655, 1086
BstDSI CCRYGG 1 cut(s) 841
BstENI CCTNNNNNAGG 1 cut(s) 857
BstF5I GGATG 4 cut(s) 328, 506, 541, 758
BstFNI CGCG 1 cut(s) 85
BstH2I RGCGCY 1 cut(s) 891
BstHHI GCGC 3 cut(s) 11, 232, 890
BstKTI GATC 4 cut(s) 142, 206, 426, 852
BstMBI GATC 4 cut(s) 139, 203, 423, 849
BstMWI GCNNNNNNNGC 4 cut(s) 229, 344, 885, 894
BstNI CCWGG 5 cut(s) 355, 542, 632, 976, 1063
BstNSI RCATGY 2 cut(s) 342, 708
BstSCI CCNGG 8 cut(s) 90, 353, 531, 540, 630, 974, 1056, 1061
BstSFI CTRYAG 2 cut(s) 660, 825
BstUI CGCG 1 cut(s) 85
BstV1I GCAGC 4 cut(s) 811, 839, 920, 974
BstX2I RGATCY 2 cut(s) 139, 203
BstXI CCANNNNNNTGG 1 cut(s) 517
BstYI RGATCY 2 cut(s) 139, 203
BsuI GTATCC 1 cut(s) 187
BsuRI GGCC 9 cut(s) 126, 608, 630, 677, 719, 734, 846, 1061, 1092
BtgI CCRYGG 1 cut(s) 841
BtgZI GCGATG 2 cut(s) 144, 1137
BtsCI GGATG 4 cut(s) 328, 506, 541, 758
BtsI GCAGTG 1 cut(s) 739
BtsIMutI CAGTG 1 cut(s) 739
Cac8I GCNNGC 2 cut(s) 228, 943
CfoI GCGC 3 cut(s) 11, 232, 890
Cfr13I GGNCC 1 cut(s) 1090
CseI GACGC 1 cut(s) 104
Csp6I GTAC 2 cut(s) 241, 365
CviAII CATG 3 cut(s) 339, 705, 876
CviQI GTAC 2 cut(s) 241, 365
DdeI CTNAG 6 cut(s) 119, 181, 267, 370, 655, 1086
DpnI GATC 4 cut(s) 141, 205, 425, 851
DpnII GATC 4 cut(s) 139, 203, 423, 849
DriI GACNNNNNGTC 1 cut(s) 154
Eam1104I CTCTTC 1 cut(s) 19
Eam1105I GACNNNNNGTC 1 cut(s) 154
EarI CTCTTC 1 cut(s) 19
Eco130I CCWWGG 1 cut(s) 819
Eco147I AGGCCT 2 cut(s) 126, 734
Eco24I GRGCYC 1 cut(s) 264
Eco47III AGCGCT 1 cut(s) 889
Eco57I CTGAAG 2 cut(s) 78, 450
EcoNI CCTNNNNNAGG 1 cut(s) 857
EcoRI GAATTC 2 cut(s) 299, 521
EcoRII CCWGG 5 cut(s) 353, 540, 630, 974, 1061
EcoT14I CCWWGG 1 cut(s) 819
EcoT38I GRGCYC 1 cut(s) 264
ErhI CCWWGG 1 cut(s) 819
FaeI CATG 3 cut(s) 342, 708, 879
FalI AAGNNNNNCTT 2 cut(s) 929, 961
FaqI GGGAC 2 cut(s) 38, 59
FatI CATG 3 cut(s) 338, 704, 875
FauI CCCGC 1 cut(s) 767
FblI GTMKAC 2 cut(s) 80, 489
Fnu4HI GCNGC 5 cut(s) 224, 825, 828, 909, 963
FokI GGATG 4 cut(s) 335, 493, 548, 765
FriOI GRGCYC 1 cut(s) 264
Fsp4HI GCNGC 5 cut(s) 224, 825, 828, 909, 963
FspBI CTAG 1 cut(s) 626
GlaI GCGC 3 cut(s) 10, 231, 889
GluI GCNGC 5 cut(s) 224, 825, 828, 909, 963
GsuI CTGGAG 4 cut(s) 254, 305, 951, 1128
HaeII RGCGCY 1 cut(s) 891
HaeIII GGCC 9 cut(s) 126, 608, 630, 677, 719, 734, 846, 1061, 1092
HapII CCGG 3 cut(s) 92, 532, 1058
HgaI GACGC 1 cut(s) 104
HhaI GCGC 3 cut(s) 11, 232, 890
Hin1II CATG 3 cut(s) 342, 708, 879
Hin6I GCGC 3 cut(s) 9, 230, 888
HinP1I GCGC 3 cut(s) 9, 230, 888
HincII GTYRAC 3 cut(s) 48, 148, 490
HindII GTYRAC 3 cut(s) 48, 148, 490
HinfI GANTC 4 cut(s) 87, 470, 815, 1067
HpaII CCGG 3 cut(s) 92, 532, 1058
Hpy166II GTNNAC 5 cut(s) 48, 81, 148, 490, 710
Hpy188I TCNGA 9 cut(s) 103, 174, 217, 406, 469, 672, 928, 1041, 1072
Hpy188III TCNNGA 5 cut(s) 284, 565, 750, 812, 1145
Hpy8I GTNNAC 5 cut(s) 48, 81, 148, 490, 710
Hpy99I CGWCG 1 cut(s) 1133
HpyAV CCTTC 2 cut(s) 667, 931
HpyCH4III ACNGT 6 cut(s) 52, 156, 460, 714, 873, 1017
HpyCH4IV ACGT 1 cut(s) 882
HpyCH4V TGCA 5 cut(s) 188, 379, 827, 908, 962
HpyF10VI GCNNNNNNNGC 4 cut(s) 229, 344, 885, 894
HpyF3I CTNAG 6 cut(s) 119, 181, 267, 370, 655, 1086
HpySE526I ACGT 1 cut(s) 882
Hsp92II CATG 3 cut(s) 342, 708, 879
HspAI GCGC 3 cut(s) 9, 230, 888
Kzo9I GATC 4 cut(s) 139, 203, 423, 849
LmnI GCTCC 1 cut(s) 970
Lsp1109I GCAGC 4 cut(s) 811, 839, 920, 974
LweI GCATC 2 cut(s) 515, 743
MaeI CTAG 1 cut(s) 626
MaeII ACGT 1 cut(s) 882
MaeIII GTNAC 1 cut(s) 970
MalI GATC 4 cut(s) 141, 205, 425, 851
MboI GATC 4 cut(s) 139, 203, 423, 849
MboII GAAGA 5 cut(s) 6, 52, 456, 790, 926
MflI RGATCY 2 cut(s) 139, 203
MhlI GDGCHC 2 cut(s) 264, 1110
MluCI AATT 7 cut(s) 255, 299, 521, 571, 582, 806, 1007
MlyI GAGTC 1 cut(s) 824
MmeI TCCRAC 2 cut(s) 384, 582
MroXI GAANNNNTTC 1 cut(s) 918
MseI TTAA 4 cut(s) 254, 690, 947, 1022
MspA1I CMGCKG 1 cut(s) 911
MspI CCGG 3 cut(s) 92, 532, 1058
MspR9I CCNGG 8 cut(s) 92, 355, 533, 542, 632, 976, 1058, 1063
Mva1269I GAATGC 1 cut(s) 549
MvaI CCWGG 5 cut(s) 355, 542, 632, 976, 1063
MvnI CGCG 1 cut(s) 85
MwoI GCNNNNNNNGC 4 cut(s) 229, 344, 885, 894
NciI CCSGG 3 cut(s) 92, 533, 1058
NdeII GATC 4 cut(s) 139, 203, 423, 849
NlaIII CATG 3 cut(s) 342, 708, 879
NspI RCATGY 2 cut(s) 342, 708
PceI AGGCCT 2 cut(s) 126, 734
PctI GAATGC 1 cut(s) 549
PdmI GAANNNNTTC 1 cut(s) 918
PfeI GAWTC 3 cut(s) 87, 470, 1067
PfoI TCCNGGA 1 cut(s) 531
PkrI GCNGC 5 cut(s) 225, 826, 829, 910, 964
PleI GAGTC 1 cut(s) 823
PpsI GAGTC 1 cut(s) 823
Psp6I CCWGG 5 cut(s) 353, 540, 630, 974, 1061
PspGI CCWGG 5 cut(s) 353, 540, 630, 974, 1061
PspPI GGNCC 1 cut(s) 1090
PsrI GAACNNNNNNTAC 2 cut(s) 357, 389
PstI CTGCAG 1 cut(s) 829
PsuI RGATCY 2 cut(s) 139, 203
RsaI GTAC 2 cut(s) 242, 366
RsaNI GTAC 2 cut(s) 241, 365
SalI GTCGAC 1 cut(s) 488
SaqAI TTAA 4 cut(s) 254, 690, 947, 1022
SatI GCNGC 5 cut(s) 224, 825, 828, 909, 963
Sau3AI GATC 4 cut(s) 139, 203, 423, 849
Sau96I GGNCC 1 cut(s) 1090
ScaI AGTACT 2 cut(s) 242, 366
SchI GAGTC 1 cut(s) 824
ScrFI CCNGG 8 cut(s) 92, 355, 533, 542, 632, 976, 1058, 1063
SduI GDGCHC 2 cut(s) 264, 1110
SfaNI GCATC 2 cut(s) 515, 743
SfcI CTRYAG 2 cut(s) 660, 825
SmlI CTYRAG 2 cut(s) 500, 812
SmoI CTYRAG 2 cut(s) 500, 812
Sse9I AATT 7 cut(s) 255, 299, 521, 571, 582, 806, 1007
SseBI AGGCCT 2 cut(s) 126, 734
SsiI CCGC 3 cut(s) 223, 774, 911
SspMI CTAG 1 cut(s) 626
StuI AGGCCT 2 cut(s) 126, 734
StyD4I CCNGG 8 cut(s) 90, 353, 531, 540, 630, 974, 1056, 1061
StyI CCWWGG 1 cut(s) 819
TaaI ACNGT 6 cut(s) 52, 156, 460, 714, 873, 1017
TaiI ACGT 1 cut(s) 885
TaqI TCGA 5 cut(s) 31, 432, 446, 489, 1053
TasI AATT 7 cut(s) 255, 299, 521, 571, 582, 806, 1007
TatI WGTACW 2 cut(s) 240, 364
TauI GCSGC 1 cut(s) 226
TfiI GAWTC 3 cut(s) 87, 470, 1067
Tru1I TTAA 4 cut(s) 254, 690, 947, 1022
Tru9I TTAA 4 cut(s) 254, 690, 947, 1022
TscAI CASTG 1 cut(s) 746
TseI GCWGC 4 cut(s) 824, 827, 908, 962
TspDTI ATGAA 2 cut(s) 784, 882
TspRI CASTG 1 cut(s) 746
XagI CCTNNNNNAGG 1 cut(s) 857
XapI RAATTY 6 cut(s) 299, 521, 571, 582, 806, 1007
XceI RCATGY 2 cut(s) 342, 708
XmiI GTMKAC 2 cut(s) 80, 489
XmnI GAANNNNTTC 1 cut(s) 918
XspI CTAG 1 cut(s) 626
ZrmI AGTACT 2 cut(s) 242, 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.