pycom17g04150

Plasma membrane ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0002286
Physical Location & Seq
Forward (+)
2872495 .. 2878671
6177 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2838 bp
ATGGATGAGAAAGCTGTCGATTTGGACGCCGTGTTGAAGGAAGCTGTCGATTTGGAACACGTGCCACTTGAAGAAGTTTATGTGACCTTGAGGTGCAACAAACATGGGCTCACATCTGAGGCTGTTGAGCAGAGGTTGGCCATTTTTGGGTTCAACAAGCTTGAGGAGAAAAAGGAAAGTAAGGTTTTGAAGTTTTTGGGGTTTATGTGGAACCCTCTCGCATGGGTGATGGAAGCTGCAGCTATCATGGCCATAGCTCTTGCCAATGGAGGAGGAAAGCCACCGGATTGGCAAGATTTTATTGGTATCATTGCTCTGCTGATCATCAACTCAACTATAAGTTTCATAGAAGAGAATAACGCGGGAAATGCCGCTGCTGCTCTCATGGCCCGTCTCGCCCCCAAAGCCAAGGTTTTTCGAGATGGAAGGTGGATCGAGGAGGATGCTTCCGTTCTTGTCCCCGGTGATATAATCAGTATAAAGCTTGGGGACATTATTCCAGCTGATGCTCGTCTACTTGACGGCGATGCACTAAAAATTGATCAGTCCGCGCTTACAGGCGAGTCACTTCCTGTGACAAAAGGCCCTGGCGACAGCGTGTATTCAGGTTCTACATGCAAGCAGGGAGAGATTGAAGCAGTGGTGATTGCCACAGGTGTTCATACCTTTTTCGGCAAGGCAGCTCATCTTGTGGATAGCACAAATCAGCAAGGCCACTTTCAAAAGGTCTTGACTGCCATTGGGAATTTCTGTATATGTTCTATTGCGGTGGGAATGATTGTAGAGATCATAGTTATGTATCCAATTCAACACCGCGAATATCGCCCAGGAATTGACAATCTGCTGGTGTTACTCATCGGAGGAATTCCCATTGCTATGCCCACGGTTTTATCTGTCACAATGGCTATTGGTTCCCATCGCTTATCTCAGCAGGGGGCTATCACTAAAAGAATGACGGCAATTGAGGAGATGGCAGGCATGGATGTCCTTTGCAGTGACAAAACCGGAACTCTGACGTTGAATAAGCTTTCGGTTGACAAGAATCTCGTAGAGATCTTCGCTAAAGAGGTGGACGCAGATACTGTTGTTCTAATGGCAGCTCGAGCCTCCCGAATGGAAAATCAAGACGCAATAGATGCTGCTATTGTTGGAATGTTGGCTGATCCAAAAGAGGCCAGAGCCGGTATCCAAGAGGTGCACTTCCTTCCATTCAACCCAACAGATAAGCGAACGGCTCTGACATACATTGACAGTCAGGGTAAAATGCATCGTGTCAGCAAGGGTGCACCGGAGCAGATTTTGAACCTTGTACGCAATAAATCAGAGATAGAACGTAGAGTTCACACCGTGATTGATAAATTCGCGGAGCGAGGTTTACGATCCTTAGCAGTAGCATACCAGGAAGTTCCAGAGGGAAGGAAAGAGAGCCAAGGAGGGCCTTGGCAATTTATCGGCCTCTTGCCTCTGTTCGACCCACCTAGGCATGACAGCGCAGAGACAATTAGAAGGGCATTGAATCTTGGGGTAAATGTTAAGATGATTACAGGTGATCAACTGGCAATAGCGAAGGAGACAGGGCGCCGCCTGGGCATGGGAACCAACATGTATCCATCATCCAGTCTGTTAGGACAGAAGAAAGATGAGTCAATTGTCGCTCTGCCAGTTGATGAACTCATTGAAAAAGCTGATGGTTTTGCTGGTGTATTCCCTGAGCACAAGTATGAAATCGTGAAACGATTGCAAGCCAGAAAGCATATTTGTGGAATGACAGGTGACGGCGTTAATGATGCTCCTGCTCTTAAAAAGGCTGATATCGGCATTGCTGTTGCTGATGCAACTGACGCAGCTCGTAGTGCTTCGGACATTGTCCTCACCGAACCTGGCCTTAGCGTCATCATCAGCGCCGTGCTAACTAGTCGGTCCATATTCCAGAGAATGAAAAATTACACGCTTGGTTTCATGTTACTGGCACTCATATGGCAATTCGACTTCCCGCCCTTCATGGTGTTGATTATTGCTATTCTCAACGACGGTACCATCATGACGATATCAAAGGATAGAGTGAAACCGTCTCCGCAGCCGGACAGCTGGAAGCTGGCGGAGATCTTTACAACTGGAATCGTCCTCGGTGGTTACTTGGCTATGATGACAGTCATTTTCTTTTGGGCAGCATACGATACAAATTTCTTTCCGCGTACCTTTGGGGTTTCAAGCCTTCACCATAAGGACACGGACGACTATAGGAAGCTCGCCGCTGTAGTTTACCTGCAAGTGAGTACCATCAGCCAGGCCCTTATATTTGTCACGCGAGCGCGGAGTTGGTCTTTCGTCGAGCGTCCTGGTCTGCTGCTTGTAGCAGCTTTTGTCATTGCTCAGTTGATTGCTACGCTGATCGCTGTCTACGCCAATTGGAGTTTCGCTGCGATTGAGGGAATCGGATGGGGGTGGGCTGGCGTGGTTTGGCTGTATAACCTCATTTTCTACTTCCCACTCGATTTTATCAAGTTCTTAATCCGATATGCCCTGAGCGGGCGAGCTTGGGATCTCGTAATTGAGCAAAGGACTGCCTTCACAAGGAAGAAGGATTTCGGGAAGGAAGAAAGGGAGCTTAAATGGGCACATGCACAGAGAACCCTCCATGGACTGCACCCACCGGCGGCCACGATGTTCAGCGATCGCACTACCTATACAGAACTCAATCAGATGGCTGAAGAGGCTAAACGACGAGCTGAAATTGCCAGGCTGAGGGAATTGCAGACCCTTAAAGGGCGTGTGGAATCGGTAGTGAGGCTGAAGAACCTGGACATAGACACAATCCAGCAGGCATACACTGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

946

Amino Acids

104.25

Weight (kDa)

6.36

Isoelectric Point (pI)

35.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_ATPase_N PF00690 20 - 83 2.6e-14 Cation transporter/ATPase, N-terminus
E1-E2_ATPase PF00122 132 - 231 5.2e-31 P-type ATPase actuator domain
Hydrolase PF00702 327 - 604 1.3e-14 haloacid dehalogenase-like hydrolase
Cation_ATPase PF13246 376 - 495 1.1e-06 P-type ATPase, cytoplasmic domain N
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2274
Acc65I GGTACC 1 cut(s) 2033
AccB1I GGYRCC 2 cut(s) 1578, 2033
AccBSI CCGCTC 1 cut(s) 2529
AccI GTMKAC 2 cut(s) 514, 2400
AccII CGCG 7 cut(s) 362, 551, 816, 1364, 2194, 2308, 2314
AclWI GGATC 4 cut(s) 440, 1157, 1374, 2550
AcoI YGGCCR 3 cut(s) 138, 249, 2658
AcsI RAATTY 4 cut(s) 745, 864, 1358, 2182
AcuI CTGAAG 2 cut(s) 2730, 2813
AcvI CACGTG 1 cut(s) 61
AcyI GRCGYC 2 cut(s) 27, 1579
AdeI CACNNNGTG 1 cut(s) 1348
AfaI GTAC 4 cut(s) 1311, 2035, 2197, 2278
AfiI CCNNNNNNNGG 7 cut(s) 147, 1591, 2529, 2574, 2656, 2745, 2766
AflIII ACRYGT 2 cut(s) 58, 1602
AhlI ACTAGT 1 cut(s) 1913
AjnI CCWGG 9 cut(s) 586, 826, 1398, 1584, 1879, 2286, 2338, 2738, 2799
AjuI GAANNNNNNNTTGG 2 cut(s) 2399, 2431
Alw21I GWGCWC 3 cut(s) 1200, 1288, 1716
Alw26I GTCTC 4 cut(s) 398, 1490, 1565, 2076
Alw44I GTGCAC 2 cut(s) 1196, 1284
AlwI GGATC 4 cut(s) 440, 1157, 1374, 2550
AlwNI CAGNNNCTG 1 cut(s) 1082
Ama87I CYCGRG 1 cut(s) 1101
ApaLI GTGCAC 2 cut(s) 1196, 1284
ApoI RAATTY 4 cut(s) 745, 864, 1358, 2182
AsiSI GCGATCGC 1 cut(s) 2677
Asp700I GAANNNNTTC 1 cut(s) 2585
Asp718I GGTACC 1 cut(s) 2033
AspA2I CCTAGG 1 cut(s) 1478
AspLEI GCGC 5 cut(s) 553, 1493, 1581, 1904, 2314
AspS9I GGNCC 5 cut(s) 388, 584, 1436, 1920, 2290
AsuC2I CCSGG 1 cut(s) 462
AsuHPI GGTGA 7 cut(s) 238, 476, 655, 1559, 1784, 1864, 2210
AvaI CYCGRG 1 cut(s) 1101
AvaII GGWCC 1 cut(s) 1920
AvrII CCTAGG 1 cut(s) 1478
BaeGI GKGCMC 3 cut(s) 1200, 1288, 2620
BaeI ACNNNNGTAYC 4 cut(s) 1071, 1104, 2017, 2050
BalI TGGCCA 2 cut(s) 140, 251
BanI GGYRCC 2 cut(s) 1578, 2033
BanII GRGCYC 1 cut(s) 111
BbrPI CACGTG 1 cut(s) 61
Bbv12I GWGCWC 3 cut(s) 1200, 1288, 1716
BbvCI CCTCAGC 1 cut(s) 2744
BceAI ACGGC 6 cut(s) 14, 538, 972, 1248, 1792, 1889
BcgI CGANNNNNNTGC 2 cut(s) 425, 459
BciT130I CCWGG 9 cut(s) 588, 828, 1400, 1586, 1881, 2288, 2340, 2740, 2801
BciVI GTATCC 3 cut(s) 810, 1196, 1617
BclI TGATCA 3 cut(s) 321, 541, 1549
BcnI CCSGG 1 cut(s) 462
BcoDI GTCTC 4 cut(s) 398, 1490, 1565, 2076
BcuI ACTAGT 1 cut(s) 1913
BfaI CTAG 2 cut(s) 1479, 1914
BfmI CTRYAG 3 cut(s) 237, 2239, 2256
BfoI RGCGCY 2 cut(s) 1582, 1905
BfuAI ACCTGC 1 cut(s) 2274
BfuI GTATCC 3 cut(s) 810, 1196, 1617
BglI GCCNNNNNGGC 1 cut(s) 1587
BglII AGATCT 2 cut(s) 1053, 2103
BlnI CCTAGG 1 cut(s) 1478
Bme18I GGWCC 1 cut(s) 1920
BmeT110I CYCGRG 1 cut(s) 1101
BmgT120I GGNCC 5 cut(s) 388, 584, 1436, 1920, 2290
BmiI GGNNCC 5 cut(s) 212, 913, 1580, 1597, 2035
BmsI GCATC 7 cut(s) 433, 496, 517, 1126, 1276, 1777, 1822
Bpu10I CCTNAGC 5 cut(s) 1384, 1710, 1886, 2525, 2744
BpuEI CTTGAG 2 cut(s) 109, 182
BpuMI CCSGG 1 cut(s) 462
BsaAI YACGTR 1 cut(s) 61
BsaHI GRCGYC 2 cut(s) 27, 1579
BsaWI WCCGGW 3 cut(s) 283, 1004, 1288
Bsc4I CCNNNNNNNGG 7 cut(s) 147, 1591, 2529, 2574, 2656, 2745, 2766
Bse118I RCCGGY 2 cut(s) 1181, 2653
Bse1I ACTGG 5 cut(s) 1560, 1617, 1661, 1971, 2119
Bse3DI GCAATG 4 cut(s) 309, 870, 1818, 2367
BseBI CCWGG 9 cut(s) 588, 828, 1400, 1586, 1881, 2288, 2340, 2740, 2801
BseGI GGATG 5 cut(s) 10, 448, 988, 1613, 2444
BseLI CCNNNNNNNGG 7 cut(s) 147, 1591, 2529, 2574, 2656, 2745, 2766
BseMI GCAATG 4 cut(s) 309, 870, 1818, 2367
BseMII CTCAG 6 cut(s) 108, 941, 1701, 2387, 2516, 2735
BseNI ACTGG 5 cut(s) 1560, 1617, 1661, 1971, 2119
BseRI GAGGAG 4 cut(s) 179, 285, 452, 980
BseSI GKGCMC 3 cut(s) 1200, 1288, 2620
BsgI GTGCAG 1 cut(s) 2630
Bsh1236I CGCG 7 cut(s) 362, 551, 816, 1364, 2194, 2308, 2314
Bsh1285I CGRYCG 1 cut(s) 2677
BshNI GGYRCC 2 cut(s) 1578, 2033
BsiEI CGRYCG 1 cut(s) 2677
BsiHKAI GWGCWC 3 cut(s) 1200, 1288, 1716
BsiHKCI CYCGRG 1 cut(s) 1101
BsiSI CCGG 7 cut(s) 284, 462, 1005, 1182, 1289, 2081, 2654
BslFI GGGAC 2 cut(s) 443, 503
BslI CCNNNNNNNGG 7 cut(s) 147, 1591, 2529, 2574, 2656, 2745, 2766
BsmAI GTCTC 4 cut(s) 398, 1490, 1565, 2076
BsmBI CGTCTC 2 cut(s) 398, 2076
BsmFI GGGAC 2 cut(s) 443, 503
BsoBI CYCGRG 1 cut(s) 1101
Bsp1286I GDGCHC 5 cut(s) 111, 1200, 1288, 1716, 2620
Bsp19I CCATGG 1 cut(s) 2638
BspCNI CTCAG 6 cut(s) 109, 940, 1702, 2386, 2517, 2736
BspFNI CGCG 7 cut(s) 362, 551, 816, 1364, 2194, 2308, 2314
BspHI TCATGA 1 cut(s) 2040
BspLI GGNNCC 5 cut(s) 212, 913, 1580, 1597, 2035
BspMAI CTGCAG 1 cut(s) 241
BspMI ACCTGC 1 cut(s) 2274
BspPI GGATC 4 cut(s) 440, 1157, 1374, 2550
BspT107I GGYRCC 2 cut(s) 1578, 2033
BsrBI CCGCTC 1 cut(s) 2529
BsrDI GCAATG 4 cut(s) 309, 870, 1818, 2367
BsrFI RCCGGY 2 cut(s) 1181, 2653
BsrI ACTGG 5 cut(s) 1560, 1617, 1661, 1971, 2119
BssAI RCCGGY 2 cut(s) 1181, 2653
BssNI GRCGYC 2 cut(s) 27, 1579
BssT1I CCWWGG 5 cut(s) 408, 1429, 1439, 1478, 2638
Bst2UI CCWGG 9 cut(s) 588, 828, 1400, 1586, 1881, 2288, 2340, 2740, 2801
Bst4CI ACNGT 8 cut(s) 886, 1084, 1253, 1348, 2033, 2070, 2152, 2833
Bst6I CTCTTC 2 cut(s) 345, 2706
BstACI GRCGYC 2 cut(s) 27, 1579
BstAPI GCANNNNNTGC 1 cut(s) 1136
BstBAI YACGTR 1 cut(s) 61
BstDEI CTNAG 8 cut(s) 117, 927, 1384, 1710, 1886, 2373, 2525, 2744
BstDSI CCRYGG 2 cut(s) 882, 2638
BstENI CCTNNNNNAGG 1 cut(s) 2572
BstF5I GGATG 5 cut(s) 10, 448, 988, 1613, 2444
BstFNI CGCG 7 cut(s) 362, 551, 816, 1364, 2194, 2308, 2314
BstH2I RGCGCY 2 cut(s) 1582, 1905
BstHHI GCGC 5 cut(s) 553, 1493, 1581, 1904, 2314
BstMAI GTCTC 4 cut(s) 398, 1490, 1565, 2076
BstMCI CGRYCG 1 cut(s) 2677
BstNI CCWGG 9 cut(s) 588, 828, 1400, 1586, 1881, 2288, 2340, 2740, 2801
BstNSI RCATGY 3 cut(s) 618, 1606, 2624
BstSFI CTRYAG 3 cut(s) 237, 2239, 2256
BstSLI GKGCMC 3 cut(s) 1200, 1288, 2620
BstUI CGCG 7 cut(s) 362, 551, 816, 1364, 2194, 2308, 2314
BstX2I RGATCY 3 cut(s) 1053, 2103, 2542
BstXI CCANNNNNNTGG 1 cut(s) 288
BstYI RGATCY 3 cut(s) 1053, 2103, 2542
BsuI GTATCC 3 cut(s) 810, 1196, 1617
BtgI CCRYGG 2 cut(s) 882, 2638
BtgZI GCGATG 2 cut(s) 540, 902
BtsCI GGATG 5 cut(s) 10, 448, 988, 1613, 2444
BtsI GCAGTG 2 cut(s) 645, 1000
BtsIMutI CAGTG 3 cut(s) 645, 1000, 2829
BveI ACCTGC 1 cut(s) 2274
CaiI CAGNNNCTG 1 cut(s) 1082
CciI TCATGA 1 cut(s) 2040
CfoI GCGC 5 cut(s) 553, 1493, 1581, 1904, 2314
Cfr10I RCCGGY 2 cut(s) 1181, 2653
Cfr13I GGNCC 5 cut(s) 388, 584, 1436, 1920, 2290
CseI GACGC 6 cut(s) 35, 1082, 1136, 1850, 1879, 2324
Csp6I GTAC 4 cut(s) 1310, 2034, 2196, 2277
CviQI GTAC 4 cut(s) 1310, 2034, 2196, 2277
DdeI CTNAG 8 cut(s) 117, 927, 1384, 1710, 1886, 2373, 2525, 2744
DinI GGCGCC 1 cut(s) 1580
DraIII CACNNNGTG 1 cut(s) 1348
EaeI YGGCCR 3 cut(s) 138, 249, 2658
Eam1104I CTCTTC 2 cut(s) 345, 2706
EarI CTCTTC 2 cut(s) 345, 2706
EciI GGCGGA 1 cut(s) 2114
Eco130I CCWWGG 5 cut(s) 408, 1429, 1439, 1478, 2638
Eco24I GRGCYC 1 cut(s) 111
Eco32I GATATC 2 cut(s) 1813, 2049
Eco47I GGWCC 1 cut(s) 1920
Eco57I CTGAAG 2 cut(s) 2730, 2813
Eco72I CACGTG 1 cut(s) 61
Eco88I CYCGRG 1 cut(s) 1101
EcoNI CCTNNNNNAGG 1 cut(s) 2572
EcoO109I RGGNCCY 3 cut(s) 584, 1436, 2290
EcoRI GAATTC 1 cut(s) 864
EcoRII CCWGG 9 cut(s) 586, 826, 1398, 1584, 1879, 2286, 2338, 2738, 2799
EcoRV GATATC 2 cut(s) 1813, 2049
EcoT14I CCWWGG 5 cut(s) 408, 1429, 1439, 1478, 2638
EcoT22I ATGCAT 1 cut(s) 1269
EcoT38I GRGCYC 1 cut(s) 111
EgeI GGCGCC 1 cut(s) 1580
EheI GGCGCC 1 cut(s) 1580
ErhI CCWWGG 5 cut(s) 408, 1429, 1439, 1478, 2638
Esp3I CGTCTC 2 cut(s) 398, 2076
FaqI GGGAC 2 cut(s) 443, 503
FauI CCCGC 3 cut(s) 355, 2001, 2522
FauNDI CATATG 1 cut(s) 1976
FbaI TGATCA 3 cut(s) 321, 541, 1549
FblI GTMKAC 2 cut(s) 514, 2400
FokI GGATG 5 cut(s) 17, 455, 995, 1600, 2451
FriOI GRGCYC 1 cut(s) 111
FspBI CTAG 2 cut(s) 1479, 1914
GlaI GCGC 5 cut(s) 552, 1492, 1580, 1903, 2313
HaeII RGCGCY 2 cut(s) 1582, 1905
HapII CCGG 7 cut(s) 284, 462, 1005, 1182, 1289, 2081, 2654
HgaI GACGC 6 cut(s) 35, 1082, 1136, 1850, 1879, 2324
HhaI GCGC 5 cut(s) 553, 1493, 1581, 1904, 2314
Hin1I GRCGYC 2 cut(s) 27, 1579
Hin6I GCGC 5 cut(s) 551, 1491, 1579, 1902, 2312
HinP1I GCGC 5 cut(s) 551, 1491, 1579, 1902, 2312
HincII GTYRAC 1 cut(s) 1036
HindII GTYRAC 1 cut(s) 1036
HindIII AAGCTT 3 cut(s) 158, 482, 1025
HinfI GANTC 7 cut(s) 563, 1042, 1516, 1643, 2118, 2433, 2777
HpaII CCGG 7 cut(s) 284, 462, 1005, 1182, 1289, 2081, 2654
HphI GGTGA 7 cut(s) 238, 476, 655, 1559, 1784, 1864, 2210
Hpy166II GTNNAC 9 cut(s) 515, 1036, 1072, 1198, 1286, 1342, 1376, 2263, 2401
Hpy188III TCNNGA 9 cut(s) 419, 730, 1110, 1124, 1409, 1729, 1930, 2041, 2590
Hpy8I GTNNAC 9 cut(s) 515, 1036, 1072, 1198, 1286, 1342, 1376, 2263, 2401
Hpy99I CGWCG 3 cut(s) 2033, 2333, 2727
HpyCH4III ACNGT 8 cut(s) 886, 1084, 1253, 1348, 2033, 2070, 2152, 2833
HpyCH4IV ACGT 3 cut(s) 60, 1016, 1333
HpyF3I CTNAG 8 cut(s) 117, 927, 1384, 1710, 1886, 2373, 2525, 2744
HpySE526I ACGT 3 cut(s) 60, 1016, 1333
Hsp92I GRCGYC 2 cut(s) 27, 1579
HspAI GCGC 5 cut(s) 551, 1491, 1579, 1902, 2312
KasI GGCGCC 1 cut(s) 1578
KpnI GGTACC 1 cut(s) 2037
Ksp22I TGATCA 3 cut(s) 321, 541, 1549
LmnI GCTCC 4 cut(s) 1291, 1366, 1795, 2605
LweI GCATC 7 cut(s) 433, 496, 517, 1126, 1276, 1777, 1822
MaeI CTAG 2 cut(s) 1479, 1914
MaeII ACGT 3 cut(s) 60, 1016, 1333
MbiI CCGCTC 1 cut(s) 2529
MboII GAAGA 8 cut(s) 83, 362, 1048, 1645, 2590, 2609, 2723, 2806
MfeI CAATTG 3 cut(s) 960, 1647, 2407
MflI RGATCY 3 cut(s) 1053, 2103, 2542
MhlI GDGCHC 5 cut(s) 111, 1200, 1288, 1716, 2620
MlsI TGGCCA 2 cut(s) 140, 251
MluNI TGGCCA 2 cut(s) 140, 251
Mly113I GGCGCC 1 cut(s) 1579
MlyI GAGTC 2 cut(s) 572, 1652
MmeI TCCRAC 1 cut(s) 1129
Mox20I TGGCCA 2 cut(s) 140, 251
Mph1103I ATGCAT 1 cut(s) 1269
MroXI GAANNNNTTC 1 cut(s) 2585
MscI TGGCCA 2 cut(s) 140, 251
MseI TTAA 6 cut(s) 1533, 1782, 1800, 2510, 2610, 2763
MslI CAYNNNNRTG 5 cut(s) 794, 875, 1719, 1758, 1975
Msp20I TGGCCA 2 cut(s) 140, 251
MspA1I CMGCKG 4 cut(s) 374, 503, 2088, 2255
MspI CCGG 7 cut(s) 284, 462, 1005, 1182, 1289, 2081, 2654
MunI CAATTG 3 cut(s) 960, 1647, 2407
MvaI CCWGG 9 cut(s) 588, 828, 1400, 1586, 1881, 2288, 2340, 2740, 2801
MvnI CGCG 7 cut(s) 362, 551, 816, 1364, 2194, 2308, 2314
NarI GGCGCC 1 cut(s) 1579
NciI CCSGG 1 cut(s) 462
NcoI CCATGG 1 cut(s) 2638
NdeI CATATG 1 cut(s) 1976
NlaIV GGNNCC 5 cut(s) 212, 913, 1580, 1597, 2035
NmuCI GTSAC 7 cut(s) 82, 564, 574, 895, 995, 1772, 2302
NsiI ATGCAT 1 cut(s) 1269
NspI RCATGY 3 cut(s) 618, 1606, 2624
PaeR7I CTCGAG 1 cut(s) 1101
PagI TCATGA 1 cut(s) 2040
PciI ACATGT 1 cut(s) 1602
PcsI WCGNNNNNNNCGW 1 cut(s) 1108
PdmI GAANNNNTTC 1 cut(s) 2585
PfeI GAWTC 5 cut(s) 1042, 1516, 2118, 2433, 2777
PflFI GACNNNGTC 1 cut(s) 1865
Ple19I CGATCG 1 cut(s) 2677
PleI GAGTC 2 cut(s) 571, 1651
PluTI GGCGCC 1 cut(s) 1582
PmaCI CACGTG 1 cut(s) 61
PmlI CACGTG 1 cut(s) 61
PpsI GAGTC 2 cut(s) 571, 1651
Ppu21I YACGTR 1 cut(s) 61
PscI ACATGT 1 cut(s) 1602
Psp6I CCWGG 9 cut(s) 586, 826, 1398, 1584, 1879, 2286, 2338, 2738, 2799
PspCI CACGTG 1 cut(s) 61
PspGI CCWGG 9 cut(s) 586, 826, 1398, 1584, 1879, 2286, 2338, 2738, 2799
PspN4I GGNNCC 5 cut(s) 212, 913, 1580, 1597, 2035
PspPI GGNCC 5 cut(s) 388, 584, 1436, 1920, 2290
PspXI VCTCGAGB 1 cut(s) 1101
PstI CTGCAG 1 cut(s) 241
PstNI CAGNNNCTG 1 cut(s) 1082
PsuI RGATCY 3 cut(s) 1053, 2103, 2542
PsyI GACNNNGTC 1 cut(s) 1865
PvuI CGATCG 1 cut(s) 2677
PvuII CAGCTG 2 cut(s) 503, 2088
RgaI GCGATCGC 1 cut(s) 2677
RsaI GTAC 4 cut(s) 1311, 2035, 2197, 2278
RsaNI GTAC 4 cut(s) 1310, 2034, 2196, 2277
RseI CAYNNNNRTG 5 cut(s) 794, 875, 1719, 1758, 1975
SaqAI TTAA 6 cut(s) 1533, 1782, 1800, 2510, 2610, 2763
Sau96I GGNCC 5 cut(s) 388, 584, 1436, 1920, 2290
SchI GAGTC 2 cut(s) 572, 1652
SduI GDGCHC 5 cut(s) 111, 1200, 1288, 1716, 2620
SfaAI GCGATCGC 1 cut(s) 2677
SfaNI GCATC 7 cut(s) 433, 496, 517, 1126, 1276, 1777, 1822
SfcI CTRYAG 3 cut(s) 237, 2239, 2256
SfoI GGCGCC 1 cut(s) 1580
Sfr274I CTCGAG 1 cut(s) 1101
SgfI GCGATCGC 1 cut(s) 2677
SgrAI CRCCGGYG 1 cut(s) 2653
SinI GGWCC 1 cut(s) 1920
SlaI CTCGAG 1 cut(s) 1101
SmiMI CAYNNNNRTG 5 cut(s) 794, 875, 1719, 1758, 1975
SmlI CTYRAG 3 cut(s) 88, 161, 1101
SmoI CTYRAG 3 cut(s) 88, 161, 1101
SpeI ACTAGT 1 cut(s) 1913
SspDI GGCGCC 1 cut(s) 1578
SspMI CTAG 2 cut(s) 1479, 1914
StyI CCWWGG 5 cut(s) 408, 1429, 1439, 1478, 2638
TaaI ACNGT 8 cut(s) 886, 1084, 1253, 1348, 2033, 2070, 2152, 2833
TaiI ACGT 3 cut(s) 63, 1019, 1336
TaqI TCGA 9 cut(s) 18, 48, 418, 435, 1102, 1470, 1986, 2331, 2493
TaqII GACCGA 1 cut(s) 1908
TauI GCSGC 4 cut(s) 374, 1584, 2255, 2660
TfiI GAWTC 5 cut(s) 1042, 1516, 2118, 2433, 2777
Tru1I TTAA 6 cut(s) 1533, 1782, 1800, 2510, 2610, 2763
Tru9I TTAA 6 cut(s) 1533, 1782, 1800, 2510, 2610, 2763
TscAI CASTG 3 cut(s) 645, 1000, 2836
TseFI GTSAC 7 cut(s) 82, 564, 574, 895, 995, 1772, 2302
Tsp45I GTSAC 7 cut(s) 82, 564, 574, 895, 995, 1772, 2302
TspDTI ATGAA 7 cut(s) 334, 650, 1683, 1737, 1948, 1952, 1990
TspGWI ACGGA 2 cut(s) 439, 2246
TspRI CASTG 3 cut(s) 645, 1000, 2836
Tth111I GACNNNGTC 1 cut(s) 1865
VneI GTGCAC 2 cut(s) 1196, 1284
VpaK11BI GGWCC 1 cut(s) 1920
XagI CCTNNNNNAGG 1 cut(s) 2572
XapI RAATTY 4 cut(s) 745, 864, 1358, 2182
XceI RCATGY 3 cut(s) 618, 1606, 2624
XhoI CTCGAG 1 cut(s) 1101
XmaJI CCTAGG 1 cut(s) 1478
XmiI GTMKAC 2 cut(s) 514, 2400
XmnI GAANNNNTTC 1 cut(s) 2585
XspI CTAG 2 cut(s) 1479, 1914
Zsp2I ATGCAT 1 cut(s) 1269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.