pycom17g04260

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0012436
Physical Location & Seq
Reverse (-)
2914120 .. 2916745
2626 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2115 bp
ATGACCGCTATGGCCACTACCACCACTCTCCTCCTTCTCCTGATCCTAACCGCCGCCATAACCACCGCCAGCTCAGCCCCGATCCTCGGTCTCGACACCTTCCTCACCCACCAATCCCGCCTTGACCGCCATGCCACCAACGACTCCTTCCTCTCTCTCTCCTCCACCCTCAAAAACTCCCTTTCCCATTCTACCCCTCTCTCCCACACACCCCACTCCCTCATCTTGTCTCTCCTCTCCCTCTCCCTCCCCCTCTCTCTCCACGTCCGCCTCGTCGGTCCCTCCTTCCCCCCCAGCTCCGCCGCCCTCCTCTCCTCCTTCCTCACCGCCGCCTCCCAACCCTCCAACCACTTCCACGTTATCGCCCCCTACGAATCCCTCTCCCACCGCCTCGCCGTCCAGCACTCTCTCCACCTCGACGTTTCTCACTCCCCCTCTCTCGCATCCCTCCTCACCAAAACCCTAAGCTCCGAGCTCGCCAAAACCACCTCCAGCCTCCGATCGCCGCTCCTCTCCGTCTCCTACGACTTAATCGATCAAATCATCAAGCAGGACTTCGAGAAAGAGAAGCCGGTTCATGGCGTTTACTTGTATTTCCTCGATATGGGTAAGCAATCCAAACCGTATGCTTACAATTACGGATCTGGGGACTCTTCCCCTGCATTCACCAAGTGCTTAGGCAGCATTTGGACCGGGAAAGACCGGTATATCTGGATCGATTTGGGCGCCGGACCGGTCGATTACGGCCCAGCGCTTTCAGGTGATGGGGTTTTGCCCAGAGGCGAGTTTCATCCGCTGGCGGCGTTGCACGGCCGCACCAAGGCTAAAAAGGCGCTGCTAGCTGACTTGGCTTCCTTGGTTTGGAGTGCTTACCAGGTTCTTCTCGTGCCCTCTTTGAGAATTCCTGTTCAGTTTGAGAATTCATTGATTGTTCAGTTCATACATGTTTATGGTTCCGATGGCGGTAAGGATTTGAGCGGTTTGGACTGGAAATTGATTGAGAGGACGTTTATGGATGAGGCAAATGATAATGGTTTGTTGTTAGGGGATCAGACTTTGAGTTTCAGGACTAATAGGGTGAGTTACTCGGACTGCCCGATTTGTTCATTTGCGATTTCGAGGTCTATTAACTCGTATACATCGAGGTTCTTGTTCGATAACTATACTTTGATTGCGAGTGAGTATTTGGACTCGAAGCGATTGCATCAGATATTGTCTGATTCGGCTGAGGAGTTTAGGAGAGTGGCTGGGCTCCCTGAGGAGGATTTTGCTAGGGTTCTTCCTGTTTATGTGTTCGATTTGGACTACAACATGCTGTTGTTGCTCGATAGGTATCACCAATCAGTTGCTTTTAAAGACATGGTTATTGCAGTGAGGACGAAGAACACACAGACTGTGAGTGATTATAGCTGTAATGGGCGTCATGTGTTTACGCAAACAAGAGAGCTTGAGAGACCGCTTGTTGGTTCAATTTTGCAGAGCATGTGGGGCGTGTCACCGACCCATATGCTGTGGAGCCCTAGGCACAATACTACATTGGTGGATTACACATGGAGTATCGGCCAGACTCCATTTGGGCCGTTCTCTGAGATTTCATCCTTATCATTCGTACAGAAGGATGCTGCTCGGAGAAATGTTCTTTTGACATCTCTCAATTACACCATATCGAGTGCTGTAGATGTTCTCCAATCCATAGCTGCACATGGCGGGGAAAGAAAGCTCCTTAAGCGTAGTCGACATGTTGAATTCATACAGAGGTGGAACTTGTTCAAGTACAAGCTGGACAAAGCAGTCTCTGCAATGTCGCTTTTGGATTTTGGGATGGCTTTGTTTTATTTGCGGTCCTCAGACCACGACCTATACGCTATCCACTCTCTCGTCTATCAAGCCTCCCAAGAAGTGGAGGCATCCCTTGTATGTTTCAAAGATCCACCAGTTCCGTGGAACGGGATCTTCATGGTGGGATTTGTTATCGTTTTTGCCTTTTATCTTCATTCTAAACGAGCATCAATCTCAAACTGTACACATTTGATGCGCCAGTTCTGCTTGCTTACAAATAATACAAAATTTTCTTCCCATACGAAGATGTTCTTGTTCTGTTTCGCATGCATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

705

Amino Acids

78.56

Weight (kDa)

8.23

Isoelectric Point (pI)

42.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7906 PF25483 84 - 297 6.6e-86 Domain of unknown function (DUF7906)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012436)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28720 AT3G28720
fragaria_vesca FvH4_6g49180
malus_domestica MD09G1047700.v1.1 MD17G1047800.v1.1
prunus_persica Prupe.3G271300_v2.0.a1
pyrus_communis pycom111g03860 pycom17g04260
rosa_chinensis RchiOBHm_Chr2g0169061
rosa_laevigata RLG00000021830
rosa_multiflora Rmu_co8387085.1_g000001 Rmu_ssc0000393.1_g000028
rosa_roxburghii Rroxscaffold_2G00082500
rosa_rugosa Rorug02G0536600
rosa_samantha Rh2AG608400 Rh2BG621200 Rh2CG590000 Rh2DG631900
rosa_wichuraiana Rw2G050440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1790
AccB1I GGYRCC 1 cut(s) 725
AccB7I CCANNNNNTGG 1 cut(s) 1900
AccBSI CCGCTC 2 cut(s) 508, 978
AccI GTMKAC 2 cut(s) 1136, 1735
AclWI GGATC 7 cut(s) 37, 76, 649, 722, 1056, 1922, 1958
AcoI YGGCCR 3 cut(s) 12, 811, 1561
AcsI RAATTY 4 cut(s) 900, 919, 1745, 2066
AcyI GRCGYC 2 cut(s) 726, 1420
AdeI CACNNNGTG 1 cut(s) 672
AfaI GTAC 3 cut(s) 1611, 1775, 2023
AfeI AGCGCT 1 cut(s) 753
AfiI CCNNNNNNNGG 9 cut(s) 86, 578, 604, 820, 861, 1261, 1463, 1900, 1946
AflII CTTAAG 1 cut(s) 1724
AflIII ACRYGT 2 cut(s) 943, 1738
AgeI ACCGGT 2 cut(s) 702, 733
AgsI TTSAA 4 cut(s) 1470, 1745, 1771, 1924
AjiI CACGTC 1 cut(s) 265
AjnI CCWGG 1 cut(s) 873
AjuI GAANNNNNNNTTGG 2 cut(s) 131, 163
Alw21I GWGCWC 1 cut(s) 477
Alw26I GTCTC 5 cut(s) 95, 234, 523, 1447, 1798
AlwI GGATC 7 cut(s) 37, 76, 649, 722, 1056, 1922, 1958
AlwNI CAGNNNCTG 1 cut(s) 1796
Aor51HI AGCGCT 1 cut(s) 753
AoxI GGCC 5 cut(s) 12, 745, 811, 1561, 1577
ApeKI GCWGC 4 cut(s) 681, 835, 1622, 1697
ApoI RAATTY 4 cut(s) 900, 919, 1745, 2066
AsiGI ACCGGT 2 cut(s) 702, 733
Asp700I GAANNNNTTC 2 cut(s) 1766, 2087
AspA2I CCTAGG 1 cut(s) 1520
AspLEI GCGC 4 cut(s) 728, 754, 835, 2037
AspS9I GGNCC 6 cut(s) 278, 690, 731, 746, 1577, 1842
AsuC2I CCSGG 1 cut(s) 694
AsuHPI GGTGA 8 cut(s) 97, 316, 445, 658, 773, 1090, 1328, 1488
AsuNHI GCTAGC 1 cut(s) 838
AvaII GGWCC 4 cut(s) 278, 690, 731, 1842
AvrII CCTAGG 1 cut(s) 1520
AxyI CCTNAGG 1 cut(s) 1257
BaeGI GKGCMC 1 cut(s) 891
BalI TGGCCA 1 cut(s) 14
BanI GGYRCC 1 cut(s) 725
BanII GRGCYC 3 cut(s) 477, 1254, 1520
BauI CACGAG 1 cut(s) 884
Bbv12I GWGCWC 1 cut(s) 477
BbvCI CCTCAGC 1 cut(s) 1227
BbvI GCAGC 4 cut(s) 693, 822, 1609, 1684
BccI CCATC 3 cut(s) 758, 953, 1816
BceAI ACGGC 4 cut(s) 380, 760, 826, 1564
BcgI CGANNNNNNTGC 4 cut(s) 1183, 1217, 1489, 1523
BciT130I CCWGG 1 cut(s) 875
BcnI CCSGG 1 cut(s) 694
BcoDI GTCTC 5 cut(s) 95, 234, 523, 1447, 1798
BfaI CTAG 3 cut(s) 839, 1272, 1521
BfmI CTRYAG 1 cut(s) 1674
BfoI RGCGCY 3 cut(s) 729, 755, 836
BfrI CTTAAG 1 cut(s) 1724
BlnI CCTAGG 1 cut(s) 1520
BlpI GCTNAGC 1 cut(s) 73
Bme1390I CCNGG 2 cut(s) 694, 875
Bme18I GGWCC 4 cut(s) 278, 690, 731, 1842
BmgBI CACGTC 1 cut(s) 265
BmgT120I GGNCC 6 cut(s) 278, 690, 731, 746, 1577, 1842
BmiI GGNNCC 5 cut(s) 280, 727, 955, 1253, 1517
BmrFI CCNGG 2 cut(s) 694, 875
BmsI GCATC 6 cut(s) 452, 1213, 1609, 1916, 2015, 2022
BmtI GCTAGC 1 cut(s) 842
BpmI CTGGAG 1 cut(s) 475
Bpu10I CCTNAGC 3 cut(s) 464, 676, 1227
Bpu1102I GCTNAGC 1 cut(s) 73
BpuEI CTTGAG 1 cut(s) 1469
BpuMI CCSGG 1 cut(s) 694
Bsa29I ATCGAT 2 cut(s) 534, 717
BsaBI GATNNNNATC 1 cut(s) 1332
BsaHI GRCGYC 2 cut(s) 726, 1420
BsaI GGTCTC 2 cut(s) 95, 1447
BsaJI CCNNGG 5 cut(s) 85, 819, 855, 1520, 1940
BsaWI WCCGGW 2 cut(s) 702, 733
Bsc4I CCNNNNNNNGG 9 cut(s) 86, 578, 604, 820, 861, 1261, 1463, 1900, 1946
Bse118I RCCGGY 3 cut(s) 571, 702, 733
Bse1I ACTGG 3 cut(s) 992, 1934, 2038
Bse21I CCTNAGG 1 cut(s) 1257
Bse3DI GCAATG 1 cut(s) 1806
Bse8I GATNNNNATC 1 cut(s) 1332
BseBI CCWGG 1 cut(s) 875
BseCI ATCGAT 2 cut(s) 534, 717
BseDI CCNNGG 5 cut(s) 85, 819, 855, 1520, 1940
BseGI GGATG 7 cut(s) 443, 790, 1021, 1595, 1624, 1827, 1907
BseJI GATNNNNATC 1 cut(s) 1332
BseLI CCNNNNNNNGG 9 cut(s) 86, 578, 604, 820, 861, 1261, 1463, 1900, 1946
BseMI GCAATG 1 cut(s) 1806
BseMII CTCAG 5 cut(s) 87, 1218, 1248, 1578, 1860
BseNI ACTGG 3 cut(s) 992, 1934, 2038
BseRI GAGGAG 9 cut(s) 20, 151, 224, 299, 304, 440, 500, 1244, 1274
BseSI GKGCMC 1 cut(s) 891
BseX3I CGGCCG 1 cut(s) 811
BseXI GCAGC 4 cut(s) 693, 822, 1609, 1684
BseYI CCCAGC 3 cut(s) 293, 748, 1247
BsgI GTGCAG 1 cut(s) 1683
Bsh1285I CGRYCG 3 cut(s) 503, 738, 814
BshFI GGCC 5 cut(s) 14, 747, 813, 1563, 1579
BshNI GGYRCC 1 cut(s) 725
BshTI ACCGGT 2 cut(s) 702, 733
BshVI ATCGAT 2 cut(s) 534, 717
BsiEI CGRYCG 3 cut(s) 503, 738, 814
BsiHKAI GWGCWC 1 cut(s) 477
BsiSI CCGG 5 cut(s) 572, 693, 703, 729, 734
BslFI GGGAC 2 cut(s) 264, 662
BslI CCNNNNNNNGG 9 cut(s) 86, 578, 604, 820, 861, 1261, 1463, 1900, 1946
BsmAI GTCTC 5 cut(s) 95, 234, 523, 1447, 1798
BsmBI CGTCTC 1 cut(s) 523
BsmFI GGGAC 2 cut(s) 264, 662
BsmI GAATGC 1 cut(s) 662
BsnI GGCC 5 cut(s) 14, 747, 813, 1563, 1579
Bso31I GGTCTC 2 cut(s) 95, 1447
Bsp1286I GDGCHC 4 cut(s) 477, 891, 1254, 1520
Bsp1407I TGTACA 1 cut(s) 2021
Bsp143I GATC 9 cut(s) 42, 81, 500, 535, 641, 714, 1048, 1927, 1950
Bsp1720I GCTNAGC 1 cut(s) 73
BspANI GGCC 5 cut(s) 14, 747, 813, 1563, 1579
BspCNI CTCAG 5 cut(s) 86, 1219, 1249, 1579, 1859
BspDI ATCGAT 2 cut(s) 534, 717
BspLI GGNNCC 5 cut(s) 280, 727, 955, 1253, 1517
BspOI GCTAGC 1 cut(s) 842
BspPI GGATC 7 cut(s) 37, 76, 649, 722, 1056, 1922, 1958
BspT107I GGYRCC 1 cut(s) 725
BspTI CTTAAG 1 cut(s) 1724
BspTNI GGTCTC 2 cut(s) 95, 1447
BsrBI CCGCTC 2 cut(s) 508, 978
BsrDI GCAATG 1 cut(s) 1806
BsrFI RCCGGY 3 cut(s) 571, 702, 733
BsrGI TGTACA 1 cut(s) 2021
BsrI ACTGG 3 cut(s) 992, 1934, 2038
BssAI RCCGGY 3 cut(s) 571, 702, 733
BssECI CCNNGG 5 cut(s) 85, 819, 855, 1520, 1940
BssMI GATC 9 cut(s) 42, 81, 500, 535, 641, 714, 1048, 1927, 1950
BssNAI GTATAC 1 cut(s) 1137
BssNI GRCGYC 2 cut(s) 726, 1420
BssSI CACGAG 1 cut(s) 884
BssT1I CCWWGG 3 cut(s) 819, 855, 1520
Bst1107I GTATAC 1 cut(s) 1137
Bst2BI CACGAG 1 cut(s) 884
Bst2UI CCWGG 1 cut(s) 875
Bst4CI ACNGT 3 cut(s) 624, 1396, 2021
Bst6I CTCTTC 1 cut(s) 658
BstACI GRCGYC 2 cut(s) 726, 1420
BstAFI CTTAAG 1 cut(s) 1724
BstAPI GCANNNNNTGC 1 cut(s) 1796
BstAUI TGTACA 1 cut(s) 2021
BstC8I GCNNGC 6 cut(s) 70, 477, 798, 840, 2048, 2107
BstDEI CTNAG 7 cut(s) 73, 464, 676, 1227, 1257, 1587, 1846
BstDSI CCRYGG 1 cut(s) 1940
BstF5I GGATG 7 cut(s) 443, 790, 1021, 1595, 1624, 1827, 1907
BstH2I RGCGCY 3 cut(s) 729, 755, 836
BstHHI GCGC 4 cut(s) 728, 754, 835, 2037
BstKTI GATC 9 cut(s) 45, 84, 503, 538, 644, 717, 1051, 1930, 1953
BstMAI GTCTC 5 cut(s) 95, 234, 523, 1447, 1798
BstMBI GATC 9 cut(s) 42, 81, 500, 535, 641, 714, 1048, 1927, 1950
BstMCI CGRYCG 3 cut(s) 503, 738, 814
BstNI CCWGG 1 cut(s) 875
BstNSI RCATGY 5 cut(s) 947, 1315, 1486, 1742, 2109
BstSCI CCNGG 2 cut(s) 692, 873
BstSFI CTRYAG 1 cut(s) 1674
BstSLI GKGCMC 1 cut(s) 891
BstV1I GCAGC 4 cut(s) 693, 822, 1609, 1684
BstX2I RGATCY 3 cut(s) 641, 1927, 1950
BstXI CCANNNNNNTGG 1 cut(s) 1941
BstYI RGATCY 3 cut(s) 641, 1927, 1950
BstZ17I GTATAC 1 cut(s) 1137
BstZI CGGCCG 1 cut(s) 811
Bsu15I ATCGAT 2 cut(s) 534, 717
Bsu36I CCTNAGG 1 cut(s) 1257
BsuRI GGCC 5 cut(s) 14, 747, 813, 1563, 1579
BsuTUI ATCGAT 2 cut(s) 534, 717
BtgI CCRYGG 1 cut(s) 1940
BtrI CACGTC 1 cut(s) 265
BtsCI GGATG 7 cut(s) 443, 790, 1021, 1595, 1624, 1827, 1907
BtsI GCAGTG 1 cut(s) 1377
BtsIMutI CAGTG 1 cut(s) 1377
Cac8I GCNNGC 6 cut(s) 70, 477, 798, 840, 2048, 2107
CaiI CAGNNNCTG 1 cut(s) 1796
CfoI GCGC 4 cut(s) 728, 754, 835, 2037
Cfr10I RCCGGY 3 cut(s) 571, 702, 733
Cfr13I GGNCC 6 cut(s) 278, 690, 731, 746, 1577, 1842
ClaI ATCGAT 2 cut(s) 534, 717
CpoI CGGWCCG 1 cut(s) 731
CseI GACGC 1 cut(s) 1409
CsiI ACCWGGT 1 cut(s) 873
Csp6I GTAC 3 cut(s) 1610, 1774, 2022
CspAI ACCGGT 2 cut(s) 702, 733
CspI CGGWCCG 1 cut(s) 731
CviQI GTAC 3 cut(s) 1610, 1774, 2022
DdeI CTNAG 7 cut(s) 73, 464, 676, 1227, 1257, 1587, 1846
DinI GGCGCC 1 cut(s) 727
DpnI GATC 9 cut(s) 44, 83, 502, 537, 643, 716, 1050, 1929, 1952
DpnII GATC 9 cut(s) 42, 81, 500, 535, 641, 714, 1048, 1927, 1950
DraI TTTAAA 1 cut(s) 1354
DraIII CACNNNGTG 1 cut(s) 672
DrdI GACNNNNNNGTC 1 cut(s) 1790
DseDI GACNNNNNNGTC 1 cut(s) 1790
EaeI YGGCCR 3 cut(s) 12, 811, 1561
EagI CGGCCG 1 cut(s) 811
Eam1104I CTCTTC 1 cut(s) 658
EarI CTCTTC 1 cut(s) 658
EciI GGCGGA 2 cut(s) 257, 289
Ecl136II GAGCTC 1 cut(s) 475
EclXI CGGCCG 1 cut(s) 811
Eco130I CCWWGG 3 cut(s) 819, 855, 1520
Eco24I GRGCYC 3 cut(s) 477, 1254, 1520
Eco31I GGTCTC 2 cut(s) 95, 1447
Eco47I GGWCC 4 cut(s) 278, 690, 731, 1842
Eco47III AGCGCT 1 cut(s) 753
Eco52I CGGCCG 1 cut(s) 811
Eco53kI GAGCTC 1 cut(s) 475
Eco81I CCTNAGG 1 cut(s) 1257
EcoICRI GAGCTC 1 cut(s) 475
EcoRI GAATTC 3 cut(s) 900, 919, 1745
EcoRII CCWGG 1 cut(s) 873
EcoT14I CCWWGG 3 cut(s) 819, 855, 1520
EcoT22I ATGCAT 1 cut(s) 2111
EcoT38I GRGCYC 3 cut(s) 477, 1254, 1520
EgeI GGCGCC 1 cut(s) 727
EheI GGCGCC 1 cut(s) 727
ErhI CCWWGG 3 cut(s) 819, 855, 1520
Esp3I CGTCTC 1 cut(s) 523
FalI AAGNNNNNCTT 4 cut(s) 539, 571, 2075, 2107
FaqI GGGAC 2 cut(s) 264, 662
FauI CCCGC 2 cut(s) 125, 1700
FauNDI CATATG 1 cut(s) 1506
FblI GTMKAC 2 cut(s) 1136, 1735
FokI GGATG 7 cut(s) 430, 777, 1028, 1582, 1631, 1834, 1894
FriOI GRGCYC 3 cut(s) 477, 1254, 1520
FspBI CTAG 3 cut(s) 839, 1272, 1521
GlaI GCGC 4 cut(s) 727, 753, 834, 2036
GsaI CCCAGC 3 cut(s) 297, 752, 1251
GsuI CTGGAG 1 cut(s) 475
HaeII RGCGCY 3 cut(s) 729, 755, 836
HaeIII GGCC 5 cut(s) 14, 747, 813, 1563, 1579
HapII CCGG 5 cut(s) 572, 693, 703, 729, 734
HgaI GACGC 1 cut(s) 1409
HhaI GCGC 4 cut(s) 728, 754, 835, 2037
Hin1I GRCGYC 2 cut(s) 726, 1420
Hin6I GCGC 4 cut(s) 726, 752, 833, 2035
HinP1I GCGC 4 cut(s) 726, 752, 833, 2035
HincII GTYRAC 1 cut(s) 1736
HindII GTYRAC 1 cut(s) 1736
HinfI GANTC 6 cut(s) 143, 374, 650, 1190, 1220, 1567
HpaII CCGG 5 cut(s) 572, 693, 703, 729, 734
HphI GGTGA 8 cut(s) 97, 316, 445, 658, 773, 1090, 1328, 1488
Hpy166II GTNNAC 5 cut(s) 586, 1137, 1431, 1736, 2024
Hpy188III TCNNGA 5 cut(s) 40, 92, 559, 712, 1066
Hpy8I GTNNAC 5 cut(s) 586, 1137, 1431, 1736, 2024
Hpy99I CGWCG 2 cut(s) 278, 422
HpyAV CCTTC 6 cut(s) 44, 109, 157, 295, 328, 1609
HpyCH4III ACNGT 3 cut(s) 624, 1396, 2021
HpyCH4IV ACGT 4 cut(s) 264, 357, 420, 1007
HpyCH4V TGCA 8 cut(s) 662, 808, 1204, 1370, 1477, 1700, 1799, 2109
HpyF3I CTNAG 7 cut(s) 73, 464, 676, 1227, 1257, 1587, 1846
HpySE526I ACGT 4 cut(s) 264, 357, 420, 1007
Hsp92I GRCGYC 2 cut(s) 726, 1420
HspAI GCGC 4 cut(s) 726, 752, 833, 2035
KasI GGCGCC 1 cut(s) 725
Kzo9I GATC 9 cut(s) 42, 81, 500, 535, 641, 714, 1048, 1927, 1950
LmnI GCTCC 6 cut(s) 302, 473, 513, 1257, 1515, 1725
Lsp1109I GCAGC 4 cut(s) 693, 822, 1609, 1684
LweI GCATC 6 cut(s) 452, 1213, 1609, 1916, 2015, 2022
MabI ACCWGGT 1 cut(s) 873
MaeI CTAG 3 cut(s) 839, 1272, 1521
MaeII ACGT 4 cut(s) 264, 357, 420, 1007
MaeIII GTNAC 2 cut(s) 1082, 1494
MalI GATC 9 cut(s) 44, 83, 502, 537, 643, 716, 1050, 1929, 1952
MbiI CCGCTC 2 cut(s) 508, 978
MboI GATC 9 cut(s) 42, 81, 500, 535, 641, 714, 1048, 1927, 1950
MboII GAAGA 8 cut(s) 645, 872, 1271, 1393, 1945, 1982, 2064, 2095
MflI RGATCY 3 cut(s) 641, 1927, 1950
MhlI GDGCHC 4 cut(s) 477, 891, 1254, 1520
MlsI TGGCCA 1 cut(s) 14
MluCI AATT 8 cut(s) 634, 900, 919, 992, 1470, 1654, 1745, 2066
MluNI TGGCCA 1 cut(s) 14
Mly113I GGCGCC 1 cut(s) 726
MlyI GAGTC 4 cut(s) 137, 644, 1184, 1561
MmeI TCCRAC 1 cut(s) 369
Mox20I TGGCCA 1 cut(s) 14
Mph1103I ATGCAT 1 cut(s) 2111
MroXI GAANNNNTTC 2 cut(s) 1766, 2087
MscI TGGCCA 1 cut(s) 14
MseI TTAA 4 cut(s) 530, 1128, 1353, 1725
MslI CAYNNNNRTG 1 cut(s) 948
Msp20I TGGCCA 1 cut(s) 14
MspA1I CMGCKG 1 cut(s) 796
MspCI CTTAAG 1 cut(s) 1724
MspI CCGG 5 cut(s) 572, 693, 703, 729, 734
MspR9I CCNGG 2 cut(s) 694, 875
Mva1269I GAATGC 1 cut(s) 662
MvaI CCWGG 1 cut(s) 875
NarI GGCGCC 1 cut(s) 726
NciI CCSGG 1 cut(s) 694
NdeI CATATG 1 cut(s) 1506
NdeII GATC 9 cut(s) 42, 81, 500, 535, 641, 714, 1048, 1927, 1950
NheI GCTAGC 1 cut(s) 838
NlaIV GGNNCC 5 cut(s) 280, 727, 955, 1253, 1517
NmuCI GTSAC 1 cut(s) 1494
NsiI ATGCAT 1 cut(s) 2111
NspI RCATGY 5 cut(s) 947, 1315, 1486, 1742, 2109
PaeI GCATGC 1 cut(s) 2109
PciI ACATGT 2 cut(s) 943, 1738
PcsI WCGNNNNNNNCGW 5 cut(s) 270, 369, 531, 1094, 1139
PctI GAATGC 1 cut(s) 662
PdmI GAANNNNTTC 2 cut(s) 1766, 2087
PfeI GAWTC 2 cut(s) 374, 1220
PflMI CCANNNNNTGG 1 cut(s) 1900
PinAI ACCGGT 2 cut(s) 702, 733
Ple19I CGATCG 1 cut(s) 503
PleI GAGTC 4 cut(s) 137, 644, 1184, 1561
PluTI GGCGCC 1 cut(s) 729
PpsI GAGTC 4 cut(s) 137, 644, 1184, 1561
PscI ACATGT 2 cut(s) 943, 1738
Psp124BI GAGCTC 1 cut(s) 477
Psp6I CCWGG 1 cut(s) 873
PspFI CCCAGC 3 cut(s) 293, 748, 1247
PspGI CCWGG 1 cut(s) 873
PspN4I GGNNCC 5 cut(s) 280, 727, 955, 1253, 1517
PspPI GGNCC 6 cut(s) 278, 690, 731, 746, 1577, 1842
PstNI CAGNNNCTG 1 cut(s) 1796
PsuI RGATCY 3 cut(s) 641, 1927, 1950
PvuI CGATCG 1 cut(s) 503
RsaI GTAC 3 cut(s) 1611, 1775, 2023
RsaNI GTAC 3 cut(s) 1610, 1774, 2022
RseI CAYNNNNRTG 1 cut(s) 948
Rsr2I CGGWCCG 1 cut(s) 731
RsrII CGGWCCG 1 cut(s) 731
SacI GAGCTC 1 cut(s) 477
SalI GTCGAC 1 cut(s) 1734
SaqAI TTAA 4 cut(s) 530, 1128, 1353, 1725
Sau3AI GATC 9 cut(s) 42, 81, 500, 535, 641, 714, 1048, 1927, 1950
Sau96I GGNCC 6 cut(s) 278, 690, 731, 746, 1577, 1842
SchI GAGTC 4 cut(s) 137, 644, 1184, 1561
ScrFI CCNGG 2 cut(s) 694, 875
SduI GDGCHC 4 cut(s) 477, 891, 1254, 1520
SexAI ACCWGGT 1 cut(s) 873
SfaNI GCATC 6 cut(s) 452, 1213, 1609, 1916, 2015, 2022
SfcI CTRYAG 1 cut(s) 1674
SfoI GGCGCC 1 cut(s) 727
SinI GGWCC 4 cut(s) 278, 690, 731, 1842
SmiMI CAYNNNNRTG 1 cut(s) 948
SmlI CTYRAG 2 cut(s) 1448, 1724
SmoI CTYRAG 2 cut(s) 1448, 1724
SphI GCATGC 1 cut(s) 2109
Sse9I AATT 8 cut(s) 634, 900, 919, 992, 1470, 1654, 1745, 2066
SspDI GGCGCC 1 cut(s) 725
SspMI CTAG 3 cut(s) 839, 1272, 1521
SstI GAGCTC 1 cut(s) 477
StyD4I CCNGG 2 cut(s) 692, 873
StyI CCWWGG 3 cut(s) 819, 855, 1520
TaaI ACNGT 3 cut(s) 624, 1396, 2021
TaiI ACGT 4 cut(s) 267, 360, 423, 1010
TaqII GACCGA 2 cut(s) 77, 266
TasI AATT 8 cut(s) 634, 900, 919, 992, 1470, 1654, 1745, 2066
TatI WGTACW 2 cut(s) 1773, 2021
TauI GCSGC 6 cut(s) 56, 305, 332, 508, 803, 816
TfiI GAWTC 2 cut(s) 374, 1220
Tru1I TTAA 4 cut(s) 530, 1128, 1353, 1725
Tru9I TTAA 4 cut(s) 530, 1128, 1353, 1725
TscAI CASTG 1 cut(s) 1377
TseFI GTSAC 1 cut(s) 1494
TseI GCWGC 4 cut(s) 681, 835, 1622, 1697
Tsp45I GTSAC 1 cut(s) 1494
TspDTI ATGAA 9 cut(s) 566, 779, 912, 928, 1095, 1584, 1738, 1945, 1982
TspGWI ACGGA 3 cut(s) 505, 654, 1929
TspRI CASTG 1 cut(s) 1377
Van91I CCANNNNNTGG 1 cut(s) 1900
Vha464I CTTAAG 1 cut(s) 1724
VpaK11BI GGWCC 4 cut(s) 278, 690, 731, 1842
XapI RAATTY 4 cut(s) 900, 919, 1745, 2066
XceI RCATGY 5 cut(s) 947, 1315, 1486, 1742, 2109
XcmI CCANNNNNNNNNTGG 2 cut(s) 1571, 1938
XmaJI CCTAGG 1 cut(s) 1520
XmiI GTMKAC 2 cut(s) 1136, 1735
XmnI GAANNNNTTC 2 cut(s) 1766, 2087
XspI CTAG 3 cut(s) 839, 1272, 1521
Zsp2I ATGCAT 1 cut(s) 2111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.