pycom17g04500

Belongs to the cullin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0003908
Physical Location & Seq
Forward (+)
3075313 .. 3077342
2030 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 993 bp
ATGAGTGGTCAGAAGAAGAGGAACTTCCAAATTGAAGCCTTCAAACATCGGGTCGTGGTTGATCCGAAGTACGCTGAGAAGACGTGGTCGATCCTGGAGCATGCGATTCATGAAATTTACAATCACAATGCTAGCGGGCTCAGCTTCGAGGAGCTCTACAGGAATGCTTATAATATGGTTCTGCACAAATTTGGAGAGAAACTCTACTCCGGTTTGGTGACAACCATGACTCATCATCTAAAAGAAATATCTAAGTCAATTGAAGCTGCTCAGGGAGAGTTGTTTCTGGAAGAGCTGAACCGGAAATGGGCAGAGCATAACAAAGCGTTGCAGATGATCCGAGACATATTGATGTACATGGATAGGACCTTCATCCCAAGCACCCACAAGACCCCTGTTCATGAGCTTGGGTTGAACCTGTGGAGGGATGTTGTTATCCATGCTAGCAAAACCCGGTCTAGGCTTTTGGATACACTTCTTGAGCTTGTGCACAGAGAAAGGAGTGGGGAAGTAATTAACAGAGGCTTGATGAGGAATATTATAAAAATGTTAATGGATTTAGGTTCTACTGTTTACCAAGATGACTTTGAGAAGCATTTTCTTGAGGTCTCAGCTGATTTTTACCGCTGTGAGTCTCAAGAGTTCATAGAGTCATGCGATTGTGGGAACTATTTAAAGAAGGCTGAGAGACGCTTAACAGAAGAGATGGAGAGAGTGTCCCATTATTTGGATGCTAGAAGTGAAGCTAAGATAACTAATGTGGTGGAGAAGGAGATGATTGAAAGTCACATGAACAGACTAGTTCACATGGAGAGCTCAGGTTTAGTCAATATGCTTGTGGATGACAAATATGACGACTTGGGAAGAATGTATAGCTTGTTCCGCAGGGTGCAGAATGGACTTGTAATAGTACGAGATGTCATGACCGCTTACATCCGAGATACTGGTAAGCAGCTAATTACTGACCCAGAAAGGTTAAGGTCGATCCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 ko04340 ko04341 map04120 map04340 map04341
Pfam Domains
Protein Families
Cullin Cullin_Nedd8

Protein Analysis

331

Amino Acids

38.82

Weight (kDa)

6.61

Isoelectric Point (pI)

47.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin PF00888 29 - 322 6.4e-92 Cullin alpha solenoid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 171, 542
AccB7I CCANNNNNTGG 1 cut(s) 727
AciI CCGC 4 cut(s) 135, 625, 883, 927
AclWI GGATC 4 cut(s) 56, 85, 331, 979
AcsI RAATTY 2 cut(s) 114, 188
AfaI GTAC 3 cut(s) 71, 356, 912
AfiI CCNNNNNNNGG 4 cut(s) 307, 424, 459, 727
AgsI TTSAA 5 cut(s) 35, 43, 263, 415, 782
AhlI ACTAGT 1 cut(s) 799
AjiI CACGTC 1 cut(s) 84
AjnI CCWGG 1 cut(s) 93
Alw21I GWGCWC 3 cut(s) 156, 492, 818
Alw26I GTCTC 4 cut(s) 336, 613, 639, 682
Alw44I GTGCAC 1 cut(s) 488
AlwI GGATC 4 cut(s) 56, 85, 331, 979
ApaLI GTGCAC 1 cut(s) 488
ApeKI GCWGC 2 cut(s) 266, 952
ApoI RAATTY 2 cut(s) 114, 188
ArsI GACNNNNNNTTYG 2 cut(s) 36, 68
AspS9I GGNCC 1 cut(s) 366
AsuC2I CCSGG 1 cut(s) 454
AsuHPI GGTGA 1 cut(s) 229
AsuNHI GCTAGC 2 cut(s) 131, 443
AvaII GGWCC 1 cut(s) 366
BaeGI GKGCMC 1 cut(s) 492
BanII GRGCYC 3 cut(s) 141, 156, 818
BbsI GAAGAC 1 cut(s) 86
Bbv12I GWGCWC 3 cut(s) 156, 492, 818
BbvI GCAGC 2 cut(s) 253, 964
BccI CCATC 1 cut(s) 700
BciT130I CCWGG 1 cut(s) 95
BciVI GTATCC 1 cut(s) 463
BcnI CCSGG 1 cut(s) 454
BcoDI GTCTC 4 cut(s) 336, 613, 639, 682
BcuI ACTAGT 1 cut(s) 799
BfaI CTAG 5 cut(s) 132, 444, 459, 735, 800
BfmI CTRYAG 2 cut(s) 157, 989
BfuI GTATCC 1 cut(s) 463
BisI GCNGC 2 cut(s) 267, 953
BlpI GCTNAGC 1 cut(s) 140
BlsI GCNGC 2 cut(s) 268, 954
Bme1390I CCNGG 2 cut(s) 95, 454
Bme18I GGWCC 1 cut(s) 366
BmgBI CACGTC 1 cut(s) 84
BmgT120I GGNCC 1 cut(s) 366
BmrFI CCNGG 2 cut(s) 95, 454
BmsI GCATC 1 cut(s) 721
BmtI GCTAGC 2 cut(s) 135, 447
BpiI GAAGAC 1 cut(s) 86
BplI GAGNNNNNCTC 2 cut(s) 186, 218
BpmI CTGGAG 1 cut(s) 116
Bpu10I CCTNAGC 2 cut(s) 270, 817
Bpu1102I GCTNAGC 1 cut(s) 140
BpuEI CTTGAG 3 cut(s) 500, 621, 623
BpuMI CCSGG 1 cut(s) 454
BsaI GGTCTC 1 cut(s) 613
BsaWI WCCGGW 2 cut(s) 209, 300
BsaXI ACNNNNNCTCC 2 cut(s) 701, 731
Bsc4I CCNNNNNNNGG 4 cut(s) 307, 424, 459, 727
Bse1I ACTGG 1 cut(s) 949
BseBI CCWGG 1 cut(s) 95
BseGI GGATG 5 cut(s) 372, 433, 736, 847, 933
BseLI CCNNNNNNNGG 4 cut(s) 307, 424, 459, 727
BseMII CTCAG 6 cut(s) 66, 154, 284, 624, 675, 831
BseNI ACTGG 1 cut(s) 949
BseRI GAGGAG 1 cut(s) 164
BseSI GKGCMC 1 cut(s) 492
BseXI GCAGC 2 cut(s) 253, 964
BsgI GTGCAG 2 cut(s) 167, 911
BsiHKAI GWGCWC 3 cut(s) 156, 492, 818
BsiSI CCGG 3 cut(s) 210, 301, 454
BslFI GGGAC 1 cut(s) 703
BslI CCNNNNNNNGG 4 cut(s) 307, 424, 459, 727
BsmAI GTCTC 4 cut(s) 336, 613, 639, 682
BsmBI CGTCTC 1 cut(s) 682
BsmFI GGGAC 1 cut(s) 703
BsmI GAATGC 1 cut(s) 169
Bso31I GGTCTC 1 cut(s) 613
Bsp1286I GDGCHC 4 cut(s) 141, 156, 492, 818
Bsp1407I TGTACA 1 cut(s) 354
Bsp143I GATC 4 cut(s) 61, 90, 336, 984
Bsp1720I GCTNAGC 1 cut(s) 140
BspACI CCGC 4 cut(s) 135, 625, 883, 927
BspCNI CTCAG 6 cut(s) 67, 153, 283, 623, 676, 830
BspHI TCATGA 3 cut(s) 109, 400, 921
BspOI GCTAGC 2 cut(s) 135, 447
BspPI GGATC 4 cut(s) 56, 85, 331, 979
BspQI GCTCTTC 1 cut(s) 285
BspTNI GGTCTC 1 cut(s) 613
BsrGI TGTACA 1 cut(s) 354
BsrI ACTGG 1 cut(s) 949
BssMI GATC 4 cut(s) 61, 90, 336, 984
Bst2UI CCWGG 1 cut(s) 95
Bst4CI ACNGT 1 cut(s) 571
Bst6I CTCTTC 3 cut(s) 11, 285, 696
BstAUI TGTACA 1 cut(s) 354
BstC8I GCNNGC 4 cut(s) 102, 133, 137, 445
BstDEI CTNAG 8 cut(s) 75, 140, 252, 270, 610, 684, 747, 817
BstF5I GGATG 5 cut(s) 372, 433, 736, 847, 933
BstKTI GATC 4 cut(s) 64, 93, 339, 987
BstMAI GTCTC 4 cut(s) 336, 613, 639, 682
BstMBI GATC 4 cut(s) 61, 90, 336, 984
BstMWI GCNNNNNNNGC 2 cut(s) 141, 882
BstNI CCWGG 1 cut(s) 95
BstNSI RCATGY 1 cut(s) 104
BstSCI CCNGG 2 cut(s) 93, 452
BstSFI CTRYAG 2 cut(s) 157, 989
BstSLI GKGCMC 1 cut(s) 492
BstV1I GCAGC 2 cut(s) 253, 964
BstV2I GAAGAC 1 cut(s) 86
BsuI GTATCC 1 cut(s) 463
BtrI CACGTC 1 cut(s) 84
BtsCI GGATG 5 cut(s) 372, 433, 736, 847, 933
Cac8I GCNNGC 4 cut(s) 102, 133, 137, 445
CciI TCATGA 3 cut(s) 109, 400, 921
Cfr13I GGNCC 1 cut(s) 366
CseI GACGC 1 cut(s) 699
Csp6I GTAC 3 cut(s) 70, 355, 911
CviQI GTAC 3 cut(s) 70, 355, 911
DdeI CTNAG 8 cut(s) 75, 140, 252, 270, 610, 684, 747, 817
DpnI GATC 4 cut(s) 63, 92, 338, 986
DpnII GATC 4 cut(s) 61, 90, 336, 984
DraI TTTAAA 1 cut(s) 675
Eam1104I CTCTTC 3 cut(s) 11, 285, 696
EarI CTCTTC 3 cut(s) 11, 285, 696
Ecl136II GAGCTC 2 cut(s) 154, 816
Eco24I GRGCYC 3 cut(s) 141, 156, 818
Eco31I GGTCTC 1 cut(s) 613
Eco47I GGWCC 1 cut(s) 366
Eco53kI GAGCTC 2 cut(s) 154, 816
EcoICRI GAGCTC 2 cut(s) 154, 816
EcoO109I RGGNCCY 1 cut(s) 366
EcoRII CCWGG 1 cut(s) 93
EcoT38I GRGCYC 3 cut(s) 141, 156, 818
Esp3I CGTCTC 1 cut(s) 682
FalI AAGNNNNNCTT 2 cut(s) 8, 40
FaqI GGGAC 1 cut(s) 703
FauI CCCGC 1 cut(s) 128
Fnu4HI GCNGC 2 cut(s) 267, 953
FokI GGATG 5 cut(s) 359, 440, 743, 854, 920
FriOI GRGCYC 3 cut(s) 141, 156, 818
Fsp4HI GCNGC 2 cut(s) 267, 953
FspBI CTAG 5 cut(s) 132, 444, 459, 735, 800
GluI GCNGC 2 cut(s) 267, 953
GsuI CTGGAG 1 cut(s) 116
HapII CCGG 3 cut(s) 210, 301, 454
HgaI GACGC 1 cut(s) 699
HinfI GANTC 4 cut(s) 106, 229, 632, 650
HpaII CCGG 3 cut(s) 210, 301, 454
HphI GGTGA 1 cut(s) 229
Hpy166II GTNNAC 3 cut(s) 490, 574, 805
Hpy188I TCNGA 4 cut(s) 12, 66, 341, 938
Hpy188III TCNNGA 7 cut(s) 110, 287, 401, 479, 602, 638, 922
Hpy8I GTNNAC 3 cut(s) 490, 574, 805
HpyAV CCTTC 4 cut(s) 49, 379, 673, 763
HpyCH4III ACNGT 1 cut(s) 571
HpyCH4IV ACGT 1 cut(s) 83
HpyCH4V TGCA 4 cut(s) 184, 331, 490, 892
HpyF10VI GCNNNNNNNGC 2 cut(s) 141, 882
HpyF3I CTNAG 8 cut(s) 75, 140, 252, 270, 610, 684, 747, 817
HpySE526I ACGT 1 cut(s) 83
Kzo9I GATC 4 cut(s) 61, 90, 336, 984
LguI GCTCTTC 1 cut(s) 285
LmnI GCTCC 2 cut(s) 97, 151
Lsp1109I GCAGC 2 cut(s) 253, 964
LweI GCATC 1 cut(s) 721
MaeI CTAG 5 cut(s) 132, 444, 459, 735, 800
MaeII ACGT 1 cut(s) 83
MaeIII GTNAC 2 cut(s) 217, 785
MalI GATC 4 cut(s) 63, 92, 338, 986
MboI GATC 4 cut(s) 61, 90, 336, 984
MboII GAAGA 6 cut(s) 25, 28, 91, 302, 713, 876
MfeI CAATTG 1 cut(s) 258
MhlI GDGCHC 4 cut(s) 141, 156, 492, 818
MluCI AATT 6 cut(s) 30, 114, 188, 258, 513, 957
MlyI GAGTC 3 cut(s) 223, 641, 659
MnlI CCTC 6 cut(s) 12, 142, 417, 515, 525, 598
MseI TTAA 5 cut(s) 516, 551, 674, 695, 977
MslI CAYNNNNRTG 1 cut(s) 350
MspA1I CMGCKG 2 cut(s) 614, 627
MspI CCGG 3 cut(s) 210, 301, 454
MspR9I CCNGG 2 cut(s) 95, 454
MunI CAATTG 1 cut(s) 258
Mva1269I GAATGC 1 cut(s) 169
MvaI CCWGG 1 cut(s) 95
MwoI GCNNNNNNNGC 2 cut(s) 141, 882
NciI CCSGG 1 cut(s) 454
NdeII GATC 4 cut(s) 61, 90, 336, 984
NheI GCTAGC 2 cut(s) 131, 443
NmuCI GTSAC 2 cut(s) 217, 785
NspI RCATGY 1 cut(s) 104
PaeI GCATGC 1 cut(s) 104
PagI TCATGA 3 cut(s) 109, 400, 921
PciSI GCTCTTC 1 cut(s) 285
PctI GAATGC 1 cut(s) 169
PfeI GAWTC 1 cut(s) 106
PflFI GACNNNGTC 1 cut(s) 85
PflMI CCANNNNNTGG 1 cut(s) 727
PfoI TCCNGGA 1 cut(s) 93
PkrI GCNGC 2 cut(s) 268, 954
PleI GAGTC 3 cut(s) 223, 640, 658
PpsI GAGTC 3 cut(s) 223, 640, 658
PpuMI RGGWCCY 1 cut(s) 366
PsiI TTATAA 2 cut(s) 171, 542
Psp124BI GAGCTC 2 cut(s) 156, 818
Psp5II RGGWCCY 1 cut(s) 366
Psp6I CCWGG 1 cut(s) 93
PspGI CCWGG 1 cut(s) 93
PspPI GGNCC 1 cut(s) 366
PspPPI RGGWCCY 1 cut(s) 366
PsrI GAACNNNNNNTAC 2 cut(s) 863, 895
PsyI GACNNNGTC 1 cut(s) 85
PvuII CAGCTG 1 cut(s) 614
RsaI GTAC 3 cut(s) 71, 356, 912
RsaNI GTAC 3 cut(s) 70, 355, 911
RseI CAYNNNNRTG 1 cut(s) 350
SacI GAGCTC 2 cut(s) 156, 818
SapI GCTCTTC 1 cut(s) 285
SaqAI TTAA 5 cut(s) 516, 551, 674, 695, 977
SatI GCNGC 2 cut(s) 267, 953
Sau3AI GATC 4 cut(s) 61, 90, 336, 984
Sau96I GGNCC 1 cut(s) 366
SchI GAGTC 3 cut(s) 223, 641, 659
ScrFI CCNGG 2 cut(s) 95, 454
SduI GDGCHC 4 cut(s) 141, 156, 492, 818
SfaNI GCATC 1 cut(s) 721
SfcI CTRYAG 2 cut(s) 157, 989
SinI GGWCC 1 cut(s) 366
SmiMI CAYNNNNRTG 1 cut(s) 350
SmlI CTYRAG 3 cut(s) 479, 602, 636
SmoI CTYRAG 3 cut(s) 479, 602, 636
SpeI ACTAGT 1 cut(s) 799
SphI GCATGC 1 cut(s) 104
Sse9I AATT 6 cut(s) 30, 114, 188, 258, 513, 957
SsiI CCGC 4 cut(s) 135, 625, 883, 927
SspI AATATT 1 cut(s) 538
SspMI CTAG 5 cut(s) 132, 444, 459, 735, 800
SstI GAGCTC 2 cut(s) 156, 818
StyD4I CCNGG 2 cut(s) 93, 452
TaaI ACNGT 1 cut(s) 571
TaiI ACGT 1 cut(s) 86
TaqI TCGA 3 cut(s) 89, 147, 983
TasI AATT 6 cut(s) 30, 114, 188, 258, 513, 957
TatI WGTACW 1 cut(s) 354
TfiI GAWTC 1 cut(s) 106
Tru1I TTAA 5 cut(s) 516, 551, 674, 695, 977
Tru9I TTAA 5 cut(s) 516, 551, 674, 695, 977
TseFI GTSAC 2 cut(s) 217, 785
TseI GCWGC 2 cut(s) 266, 952
Tsp45I GTSAC 2 cut(s) 217, 785
TspDTI ATGAA 6 cut(s) 98, 126, 361, 389, 634, 806
Tth111I GACNNNGTC 1 cut(s) 85
Van91I CCANNNNNTGG 1 cut(s) 727
VneI GTGCAC 1 cut(s) 488
VpaK11BI GGWCC 1 cut(s) 366
XapI RAATTY 2 cut(s) 114, 188
XceI RCATGY 1 cut(s) 104
XspI CTAG 5 cut(s) 132, 444, 459, 735, 800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.