pycom17g07550

Regulator of chromosome condensation (RCC1) repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0008433
Physical Location & Seq
Reverse (-)
5498426 .. 5501532
3107 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1176 bp
ATGGTATCCGATACCACCATTGTCACTTGGGGCTCTGGGGAAGACGGCCAATTAGGAATCGGGAATAACGAGGAGAAGGAATGGGTGTGCGCCGTCAAAGCCCTCGAGTCGCAGACTGTCCGCTCTGTTGTCGCTGGCAGCCGAAACTCCCTCGCCATTTGCGATGATGGCAAGTTGTTTACATGGGGTTGGAACCAGAGAGGAACCCTGGGGCATCCTCCTGAGACCAAAACTGAGAACATTCCAAGCCAGGTTAAAGCTCTGGCCAATGTCAAAATTGTTCAGGCTGCTATTGGTGGATGGCATTGTTTGGCTGTTGATGATCAAGGCCGCGCTTACGCCTGGGGTGGAAACGAGTATGGACAGTGTGGTGAGGAACCAGAGCGGAAGGATGACACTGGTAGACCTTTGAGAAGGGATATAGTGATTCCTCAACGCTGTGCACCGAAACTTAAAGTTCGCCAGGTAGCTGCTGGTGGTACTCACTCTGTGGTACTTACACGCGACGGGCAAGTCTGGACGTGGGGTCAGCCATGGCCTCCGGGAGACATAAAACAAATTTCAACACCTGTGCGAGTACAAGGTCTTGATGCAGTGAGGCTCATTGCAGTAGGGGCATTTCATAATTTGGCTCTGCAAGAGGATGGTGCCTTATGGGCTTGGGGTAACAACGAATATGGACAACTTGGAACAGAGGATACTCAGCCGAGATCACAACCTATTCCTGTCCAAGGACTTTCTGGTCTTACTTTGGTGGATATTGCTGCTGGAGGATGGCATTCTACTGCACTAACAGAAGATGGAGAGGTGTATGGTTGGGGACGAGGAGAACATGGGAGGCTTGGGTTTGGCGATAATGATAAGAGCAGCAAAATGGTGCCGCAAAGGGTTAATCTTTTAGCTGGGGAGGATATTGTTCAGGTGTCTTGTGGAGGCACTCACTCTGTTGCACTAACTCGTGACGGGCGAATGTTTTCGTTTGGCCGAGGCGACCATGGACGCCTTGGATATGGAAGGAAGGTGACGACTGGTCAGCCGATGGAAGTTCCCATCGGCATCCCTCCCAGACATGGCACTGCGGACAATGGAAATTGGATTGCCAAACTCGTTGCTTGCGGAGGACGCCATACGTTGGCAATCGTAGAATGGAAAGCTGATGATGAATCAGAATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RCC1

Protein Analysis

392

Amino Acids

42.1

Weight (kDa)

5.84

Isoelectric Point (pI)

27.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alsin_RLD PF25384 4 - 112 1.1e-09 Alsin RCC1-like domain
WD40_RLD PF25390 7 - 379 7.5e-93 RCC1-like domain
RCC1 PF00415 9 - 53 4.1e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 40 - 68 4.9e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 56 - 106 2.2e-15 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 93 - 122 2.1e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 109 - 166 2.8e-09 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 153 - 177 1.3e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 199 - 228 1.6e-09 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 215 - 264 5.9e-15 Regulator of chromosome condensation (RCC1) repeat
Alsin_RLD PF25384 218 - 289 4.9e-06 Alsin RCC1-like domain
RCC1_2 PF13540 252 - 279 3.1e-10 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 267 - 318 2.3e-17 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 305 - 333 7.9e-10 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 321 - 380 2.5e-09 Regulator of chromosome condensation (RCC1) repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 512, 741
AccB1I GGYRCC 2 cut(s) 647, 877
AccB7I CCANNNNNTGG 1 cut(s) 1132
AccBSI CCGCTC 2 cut(s) 123, 385
AccI GTMKAC 1 cut(s) 403
AccII CGCG 2 cut(s) 333, 504
AciI CCGC 6 cut(s) 121, 331, 385, 881, 1079, 1116
AcoI YGGCCR 3 cut(s) 46, 264, 982
AcsI RAATTY 1 cut(s) 558
AcyI GRCGYC 2 cut(s) 1000, 1123
AdeI CACNNNGTG 1 cut(s) 490
AfaI GTAC 3 cut(s) 481, 495, 579
AfiI CCNNNNNNNGG 3 cut(s) 731, 1070, 1132
AgsI TTSAA 1 cut(s) 564
AhdI GACNNNNNGTC 2 cut(s) 525, 1029
AjiI CACGTC 1 cut(s) 522
AjnI CCWGG 4 cut(s) 207, 249, 341, 462
AluBI AGCT 4 cut(s) 260, 470, 902, 1154
AluI AGCT 4 cut(s) 260, 470, 902, 1154
Alw21I GWGCWC 1 cut(s) 445
Alw26I GTCTC 2 cut(s) 218, 540
Alw44I GTGCAC 1 cut(s) 441
Ama87I CYCGRG 1 cut(s) 104
AoxI GGCC 5 cut(s) 46, 264, 328, 536, 982
ApaLI GTGCAC 1 cut(s) 441
ApeKI GCWGC 5 cut(s) 138, 287, 470, 764, 867
ApoI RAATTY 1 cut(s) 558
Asp700I GAANNNNTTC 1 cut(s) 973
AspLEI GCGC 2 cut(s) 92, 335
AsuC2I CCSGG 1 cut(s) 543
AsuHPI GGTGA 2 cut(s) 383, 1033
AvaI CYCGRG 1 cut(s) 104
BaeGI GKGCMC 1 cut(s) 445
BalI TGGCCA 1 cut(s) 266
BanI GGYRCC 2 cut(s) 647, 877
BanII GRGCYC 1 cut(s) 35
BauI CACGAG 1 cut(s) 957
BbsI GAAGAC 1 cut(s) 48
Bbv12I GWGCWC 1 cut(s) 445
BbvI GCAGC 5 cut(s) 150, 274, 457, 751, 879
BccI CCATC 7 cut(s) 161, 294, 638, 768, 794, 1033, 1058
BceAI ACGGC 2 cut(s) 61, 77
BciT130I CCWGG 4 cut(s) 209, 251, 343, 464
BciVI GTATCC 2 cut(s) 16, 691
BclI TGATCA 1 cut(s) 322
BcnI CCSGG 1 cut(s) 543
BcoDI GTCTC 2 cut(s) 218, 540
BfuI GTATCC 2 cut(s) 16, 691
BglI GCCNNNNNGGC 1 cut(s) 656
BisI GCNGC 7 cut(s) 139, 288, 331, 471, 765, 868, 881
BlsI GCNGC 7 cut(s) 140, 289, 332, 472, 766, 869, 882
Bme1390I CCNGG 5 cut(s) 209, 251, 343, 464, 543
BmeRI GACNNNNNGTC 2 cut(s) 525, 1029
BmeT110I CYCGRG 1 cut(s) 104
BmgBI CACGTC 1 cut(s) 522
BmiI GGNNCC 5 cut(s) 194, 205, 378, 649, 879
BmrFI CCNGG 5 cut(s) 209, 251, 343, 464, 543
BmsI GCATC 3 cut(s) 223, 580, 1065
BpiI GAAGAC 1 cut(s) 48
BpmI CTGGAG 1 cut(s) 789
BpuMI CCSGG 1 cut(s) 543
BsaHI GRCGYC 2 cut(s) 1000, 1123
BsaI GGTCTC 1 cut(s) 218
BsaJI CCNNGG 8 cut(s) 207, 208, 342, 533, 730, 985, 994, 1003
Bsc4I CCNNNNNNNGG 3 cut(s) 731, 1070, 1132
Bse1I ACTGG 2 cut(s) 403, 1033
Bse3DI GCAATG 1 cut(s) 603
BseBI CCWGG 4 cut(s) 209, 251, 343, 464
BseDI CCNNGG 8 cut(s) 207, 208, 342, 533, 730, 985, 994, 1003
BseGI GGATG 6 cut(s) 214, 305, 397, 649, 779, 1056
BseLI CCNNNNNNNGG 3 cut(s) 731, 1070, 1132
BseMI GCAATG 1 cut(s) 603
BseMII CTCAG 3 cut(s) 213, 225, 716
BseNI ACTGG 2 cut(s) 403, 1033
BseRI GAGGAG 2 cut(s) 86, 840
BseSI GKGCMC 1 cut(s) 445
BseXI GCAGC 5 cut(s) 150, 274, 457, 751, 879
BseYI CCCAGC 1 cut(s) 902
BsgI GTGCAG 1 cut(s) 771
Bsh1236I CGCG 2 cut(s) 333, 504
BshFI GGCC 5 cut(s) 48, 266, 330, 538, 984
BshNI GGYRCC 2 cut(s) 647, 877
BsiHKAI GWGCWC 1 cut(s) 445
BsiHKCI CYCGRG 1 cut(s) 104
BsiSI CCGG 1 cut(s) 542
BslFI GGGAC 1 cut(s) 834
BslI CCNNNNNNNGG 3 cut(s) 731, 1070, 1132
BsmAI GTCTC 2 cut(s) 218, 540
BsmFI GGGAC 1 cut(s) 834
BsmI GAATGC 1 cut(s) 778
BsnI GGCC 5 cut(s) 48, 266, 330, 538, 984
Bso31I GGTCTC 1 cut(s) 218
BsoBI CYCGRG 1 cut(s) 104
Bsp1286I GDGCHC 2 cut(s) 35, 445
Bsp143I GATC 2 cut(s) 322, 710
Bsp19I CCATGG 2 cut(s) 533, 994
BspACI CCGC 6 cut(s) 121, 331, 385, 881, 1079, 1116
BspANI GGCC 5 cut(s) 48, 266, 330, 538, 984
BspCNI CTCAG 3 cut(s) 214, 226, 715
BspFNI CGCG 2 cut(s) 333, 504
BspLI GGNNCC 5 cut(s) 194, 205, 378, 649, 879
BspT107I GGYRCC 2 cut(s) 647, 877
BspTNI GGTCTC 1 cut(s) 218
BsrBI CCGCTC 2 cut(s) 123, 385
BsrDI GCAATG 1 cut(s) 603
BsrI ACTGG 2 cut(s) 403, 1033
BssECI CCNNGG 8 cut(s) 207, 208, 342, 533, 730, 985, 994, 1003
BssMI GATC 2 cut(s) 322, 710
BssNI GRCGYC 2 cut(s) 1000, 1123
BssSI CACGAG 1 cut(s) 957
BssT1I CCWWGG 4 cut(s) 533, 730, 994, 1003
Bst2BI CACGAG 1 cut(s) 957
Bst2UI CCWGG 4 cut(s) 209, 251, 343, 464
Bst4CI ACNGT 2 cut(s) 118, 366
BstACI GRCGYC 2 cut(s) 1000, 1123
BstC8I GCNNGC 2 cut(s) 136, 1114
BstDEI CTNAG 3 cut(s) 222, 234, 702
BstDSI CCRYGG 2 cut(s) 533, 994
BstENI CCTNNNNNAGG 1 cut(s) 729
BstF5I GGATG 6 cut(s) 214, 305, 397, 649, 779, 1056
BstFNI CGCG 2 cut(s) 333, 504
BstHHI GCGC 2 cut(s) 92, 335
BstKTI GATC 2 cut(s) 325, 713
BstMAI GTCTC 2 cut(s) 218, 540
BstMBI GATC 2 cut(s) 322, 710
BstMWI GCNNNNNNNGC 5 cut(s) 98, 168, 614, 656, 1122
BstNI CCWGG 4 cut(s) 209, 251, 343, 464
BstSCI CCNGG 5 cut(s) 207, 249, 341, 462, 541
BstSLI GKGCMC 1 cut(s) 445
BstUI CGCG 2 cut(s) 333, 504
BstV1I GCAGC 5 cut(s) 150, 274, 457, 751, 879
BstV2I GAAGAC 1 cut(s) 48
BsuI GTATCC 2 cut(s) 16, 691
BsuRI GGCC 5 cut(s) 48, 266, 330, 538, 984
BtgI CCRYGG 2 cut(s) 533, 994
BtgZI GCGATG 1 cut(s) 177
BtrI CACGTC 1 cut(s) 522
BtsCI GGATG 6 cut(s) 214, 305, 397, 649, 779, 1056
BtsI GCAGTG 2 cut(s) 600, 1074
BtsIMutI CAGTG 4 cut(s) 371, 396, 600, 1074
Cac8I GCNNGC 2 cut(s) 136, 1114
CfoI GCGC 2 cut(s) 92, 335
CseI GACGC 2 cut(s) 1008, 1131
Csp6I GTAC 3 cut(s) 480, 494, 578
CviAII CATG 5 cut(s) 183, 534, 833, 995, 1070
CviQI GTAC 3 cut(s) 480, 494, 578
DdeI CTNAG 3 cut(s) 222, 234, 702
DpnI GATC 2 cut(s) 324, 712
DpnII GATC 2 cut(s) 322, 710
DraIII CACNNNGTG 1 cut(s) 490
DrdI GACNNNNNNGTC 2 cut(s) 512, 741
DriI GACNNNNNGTC 2 cut(s) 525, 1029
DseDI GACNNNNNNGTC 2 cut(s) 512, 741
EaeI YGGCCR 3 cut(s) 46, 264, 982
Eam1105I GACNNNNNGTC 2 cut(s) 525, 1029
Eco130I CCWWGG 4 cut(s) 533, 730, 994, 1003
Eco24I GRGCYC 1 cut(s) 35
Eco31I GGTCTC 1 cut(s) 218
Eco88I CYCGRG 1 cut(s) 104
EcoNI CCTNNNNNAGG 1 cut(s) 729
EcoRII CCWGG 4 cut(s) 207, 249, 341, 462
EcoT14I CCWWGG 4 cut(s) 533, 730, 994, 1003
EcoT38I GRGCYC 1 cut(s) 35
ErhI CCWWGG 4 cut(s) 533, 730, 994, 1003
FaeI CATG 5 cut(s) 186, 537, 836, 998, 1073
FaqI GGGAC 1 cut(s) 834
FatI CATG 5 cut(s) 182, 533, 832, 994, 1069
FbaI TGATCA 1 cut(s) 322
FblI GTMKAC 1 cut(s) 403
Fnu4HI GCNGC 7 cut(s) 139, 288, 331, 471, 765, 868, 881
FokI GGATG 6 cut(s) 201, 312, 404, 656, 786, 1043
FriOI GRGCYC 1 cut(s) 35
Fsp4HI GCNGC 7 cut(s) 139, 288, 331, 471, 765, 868, 881
GlaI GCGC 2 cut(s) 91, 334
GluI GCNGC 7 cut(s) 139, 288, 331, 471, 765, 868, 881
GsaI CCCAGC 1 cut(s) 906
GsuI CTGGAG 1 cut(s) 789
HaeIII GGCC 5 cut(s) 48, 266, 330, 538, 984
HapII CCGG 1 cut(s) 542
HgaI GACGC 2 cut(s) 1008, 1131
HhaI GCGC 2 cut(s) 92, 335
Hin1I GRCGYC 2 cut(s) 1000, 1123
Hin1II CATG 5 cut(s) 186, 537, 836, 998, 1073
Hin6I GCGC 2 cut(s) 90, 333
HinP1I GCGC 2 cut(s) 90, 333
HinfI GANTC 5 cut(s) 57, 107, 427, 1163, 1169
HpaII CCGG 1 cut(s) 542
HphI GGTGA 2 cut(s) 383, 1033
Hpy166II GTNNAC 3 cut(s) 180, 404, 443
Hpy188I TCNGA 2 cut(s) 10, 1168
Hpy188III TCNNGA 5 cut(s) 61, 221, 517, 587, 959
Hpy8I GTNNAC 3 cut(s) 180, 404, 443
Hpy99I CGWCG 1 cut(s) 509
HpyAV CCTTC 5 cut(s) 70, 382, 408, 1008, 1012
HpyCH4III ACNGT 2 cut(s) 118, 366
HpyCH4IV ACGT 2 cut(s) 521, 1130
HpyCH4V TGCA 6 cut(s) 443, 593, 608, 637, 788, 950
HpyF10VI GCNNNNNNNGC 5 cut(s) 98, 168, 614, 656, 1122
HpyF3I CTNAG 3 cut(s) 222, 234, 702
HpySE526I ACGT 2 cut(s) 521, 1130
Hsp92I GRCGYC 2 cut(s) 1000, 1123
Hsp92II CATG 5 cut(s) 186, 537, 836, 998, 1073
HspAI GCGC 2 cut(s) 90, 333
Ksp22I TGATCA 1 cut(s) 322
Kzo9I GATC 2 cut(s) 322, 710
Lsp1109I GCAGC 5 cut(s) 150, 274, 457, 751, 879
LweI GCATC 3 cut(s) 223, 580, 1065
MaeII ACGT 2 cut(s) 521, 1130
MaeIII GTNAC 4 cut(s) 22, 665, 959, 1021
MalI GATC 2 cut(s) 324, 712
MbiI CCGCTC 2 cut(s) 123, 385
MboI GATC 2 cut(s) 322, 710
MboII GAAGA 2 cut(s) 53, 809
MhlI GDGCHC 2 cut(s) 35, 445
MlsI TGGCCA 1 cut(s) 266
MluCI AATT 5 cut(s) 50, 276, 558, 625, 1090
MluNI TGGCCA 1 cut(s) 266
MlyI GAGTC 1 cut(s) 116
MmeI TCCRAC 1 cut(s) 170
Mox20I TGGCCA 1 cut(s) 266
MroXI GAANNNNTTC 1 cut(s) 973
MscI TGGCCA 1 cut(s) 266
MseI TTAA 3 cut(s) 255, 453, 891
Msp20I TGGCCA 1 cut(s) 266
MspI CCGG 1 cut(s) 542
MspR9I CCNGG 5 cut(s) 209, 251, 343, 464, 543
Mva1269I GAATGC 1 cut(s) 778
MvaI CCWGG 4 cut(s) 209, 251, 343, 464
MvnI CGCG 2 cut(s) 333, 504
MwoI GCNNNNNNNGC 5 cut(s) 98, 168, 614, 656, 1122
NciI CCSGG 1 cut(s) 543
NcoI CCATGG 2 cut(s) 533, 994
NdeII GATC 2 cut(s) 322, 710
NlaIII CATG 5 cut(s) 186, 537, 836, 998, 1073
NlaIV GGNNCC 5 cut(s) 194, 205, 378, 649, 879
NmeAIII GCCGAG 2 cut(s) 732, 1010
NmuCI GTSAC 3 cut(s) 22, 959, 1021
PaeR7I CTCGAG 1 cut(s) 104
PasI CCCWGGG 1 cut(s) 208
PcsI WCGNNNNNNNCGW 3 cut(s) 66, 159, 964
PctI GAATGC 1 cut(s) 778
PdmI GAANNNNTTC 1 cut(s) 973
PfeI GAWTC 4 cut(s) 57, 427, 1163, 1169
PflMI CCANNNNNTGG 1 cut(s) 1132
PfoI TCCNGGA 1 cut(s) 541
PkrI GCNGC 7 cut(s) 140, 289, 332, 472, 766, 869, 882
PleI GAGTC 1 cut(s) 115
PpsI GAGTC 1 cut(s) 115
Psp6I CCWGG 4 cut(s) 207, 249, 341, 462
PspFI CCCAGC 1 cut(s) 902
PspGI CCWGG 4 cut(s) 207, 249, 341, 462
PspN4I GGNNCC 5 cut(s) 194, 205, 378, 649, 879
PspXI VCTCGAGB 1 cut(s) 104
PsrI GAACNNNNNNTAC 2 cut(s) 682, 714
RsaI GTAC 3 cut(s) 481, 495, 579
RsaNI GTAC 3 cut(s) 480, 494, 578
SaqAI TTAA 3 cut(s) 255, 453, 891
SatI GCNGC 7 cut(s) 139, 288, 331, 471, 765, 868, 881
Sau3AI GATC 2 cut(s) 322, 710
SchI GAGTC 1 cut(s) 116
ScrFI CCNGG 5 cut(s) 209, 251, 343, 464, 543
SduI GDGCHC 2 cut(s) 35, 445
SfaNI GCATC 3 cut(s) 223, 580, 1065
Sfr274I CTCGAG 1 cut(s) 104
SlaI CTCGAG 1 cut(s) 104
SmlI CTYRAG 1 cut(s) 104
SmoI CTYRAG 1 cut(s) 104
Sse9I AATT 5 cut(s) 50, 276, 558, 625, 1090
SsiI CCGC 6 cut(s) 121, 331, 385, 881, 1079, 1116
StyD4I CCNGG 5 cut(s) 207, 249, 341, 462, 541
StyI CCWWGG 4 cut(s) 533, 730, 994, 1003
TaaI ACNGT 2 cut(s) 118, 366
TaiI ACGT 2 cut(s) 524, 1133
TaqI TCGA 1 cut(s) 105
TasI AATT 5 cut(s) 50, 276, 558, 625, 1090
TatI WGTACW 1 cut(s) 577
TauI GCSGC 2 cut(s) 333, 883
TfiI GAWTC 4 cut(s) 57, 427, 1163, 1169
Tru1I TTAA 3 cut(s) 255, 453, 891
Tru9I TTAA 3 cut(s) 255, 453, 891
TscAI CASTG 4 cut(s) 371, 403, 600, 1081
TseFI GTSAC 3 cut(s) 22, 959, 1021
TseI GCWGC 5 cut(s) 138, 287, 470, 764, 867
Tsp45I GTSAC 3 cut(s) 22, 959, 1021
TspDTI ATGAA 2 cut(s) 611, 1176
TspRI CASTG 4 cut(s) 371, 403, 600, 1081
Van91I CCANNNNNTGG 1 cut(s) 1132
VneI GTGCAC 1 cut(s) 441
XagI CCTNNNNNAGG 1 cut(s) 729
XapI RAATTY 1 cut(s) 558
XcmI CCANNNNNNNNNTGG 3 cut(s) 470, 737, 1001
XhoI CTCGAG 1 cut(s) 104
XmiI GTMKAC 1 cut(s) 403
XmnI GAANNNNTTC 1 cut(s) 973
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.