pycom17g07720

K homology RNA-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0003954
Physical Location & Seq
Reverse (-)
5626526 .. 5627132
607 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 567 bp
ATGATGCATGGGAACAATCGAAACTTTTTCAAGAAACGCCCTAATATCCAGTTTAAAAGAAGAGGGGGTAACAACAACTACAAGAAAGGAAAGTGGAATGATTCAAGCAGTAAGCAACCTTCAGAGGATTATCAATTGCTCAACACTGTGTATCGTATTCTCTGCCCTTCTAAAAAGATCGGTGGTGTTATTGGGAAGGGTAGCAACATAGTCAAAGCCCTTAAGAGGAGACCCCTGGCAAAGATTAAGGTTTCTGATTCTCTTCTTGGCTCAGATGAGAGAGTAATTATTATCTATATCTCTCCAACAAAAATTTCAAGCAAACAAAATAACCATGAAAATTTAGCTGAGGGAAATGAAATGGAGCCGCATTGTGCTGCCCAGGATGCTCTTTTGAAAGTTCATGACAGGATTGTAGAGGACGATCGTTTTGGTGGAGTTACATTTGATGATGACAACGAGAACAGTGTTGTCCTTCCTACCGATCAGCTTCCAACTTGTGGTATGGAAACTGATGAATTGGTGCTGGTACTCTTTCAATTTTTGTGCTCCTTGCATGTTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KH_1

Protein Analysis

189

Amino Acids

21.21

Weight (kDa)

8.84

Isoelectric Point (pI)

42.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KH_1 PF00013 51 - 98 2.6e-07 KH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 500
AciI CCGC 1 cut(s) 368
AcsI RAATTY 2 cut(s) 312, 340
AcuI CTGAAG 1 cut(s) 105
AfaI GTAC 1 cut(s) 531
AfiI CCNNNNNNNGG 2 cut(s) 225, 500
AflII CTTAAG 1 cut(s) 221
AgsI TTSAA 5 cut(s) 31, 105, 318, 397, 539
AjnI CCWGG 2 cut(s) 234, 381
AjuI GAANNNNNNNTTGG 2 cut(s) 298, 330
AluBI AGCT 2 cut(s) 347, 490
AluI AGCT 2 cut(s) 347, 490
Alw21I GWGCWC 1 cut(s) 551
Alw26I GTCTC 1 cut(s) 223
ApeKI GCWGC 1 cut(s) 377
ApoI RAATTY 2 cut(s) 312, 340
ArsI GACNNNNNNTTYG 2 cut(s) 413, 445
BarI GAAGNNNNNNTAC 2 cut(s) 103, 135
Bbv12I GWGCWC 1 cut(s) 551
BbvCI CCTCAGC 1 cut(s) 348
BbvI GCAGC 1 cut(s) 364
BciT130I CCWGG 2 cut(s) 236, 383
BcoDI GTCTC 1 cut(s) 223
BfrI CTTAAG 1 cut(s) 221
BisI GCNGC 2 cut(s) 368, 378
BlsI GCNGC 2 cut(s) 369, 379
Bme1390I CCNGG 2 cut(s) 236, 383
BmiI GGNNCC 1 cut(s) 366
BmrFI CCNGG 2 cut(s) 236, 383
BmsI GCATC 1 cut(s) 376
Bpu10I CCTNAGC 1 cut(s) 348
BsaI GGTCTC 1 cut(s) 223
BsaJI CCNNGG 2 cut(s) 234, 381
Bsc4I CCNNNNNNNGG 2 cut(s) 225, 500
Bse1I ACTGG 1 cut(s) 49
BseBI CCWGG 2 cut(s) 236, 383
BseDI CCNNGG 2 cut(s) 234, 381
BseGI GGATG 1 cut(s) 391
BseLI CCNNNNNNNGG 2 cut(s) 225, 500
BseMII CTCAG 2 cut(s) 285, 339
BseNI ACTGG 1 cut(s) 49
BseRI GAGGAG 1 cut(s) 241
BseXI GCAGC 1 cut(s) 364
Bsh1285I CGRYCG 1 cut(s) 427
BsiEI CGRYCG 1 cut(s) 427
BsiHKAI GWGCWC 1 cut(s) 551
BslI CCNNNNNNNGG 2 cut(s) 225, 500
BsmAI GTCTC 1 cut(s) 223
Bso31I GGTCTC 1 cut(s) 223
Bsp1286I GDGCHC 1 cut(s) 551
Bsp143I GATC 3 cut(s) 177, 424, 484
BspACI CCGC 1 cut(s) 368
BspCNI CTCAG 2 cut(s) 284, 340
BspHI TCATGA 1 cut(s) 403
BspLI GGNNCC 1 cut(s) 366
BspTI CTTAAG 1 cut(s) 221
BspTNI GGTCTC 1 cut(s) 223
BsrI ACTGG 1 cut(s) 49
BssECI CCNNGG 2 cut(s) 234, 381
BssMI GATC 3 cut(s) 177, 424, 484
Bst2UI CCWGG 2 cut(s) 236, 383
Bst4CI ACNGT 2 cut(s) 148, 467
Bst6I CTCTTC 2 cut(s) 55, 267
BstAFI CTTAAG 1 cut(s) 221
BstDEI CTNAG 2 cut(s) 271, 348
BstF5I GGATG 1 cut(s) 391
BstKTI GATC 3 cut(s) 180, 427, 487
BstMAI GTCTC 1 cut(s) 223
BstMBI GATC 3 cut(s) 177, 424, 484
BstMCI CGRYCG 1 cut(s) 427
BstMWI GCNNNNNNNGC 1 cut(s) 386
BstNI CCWGG 2 cut(s) 236, 383
BstNSI RCATGY 1 cut(s) 560
BstSCI CCNGG 2 cut(s) 234, 381
BstV1I GCAGC 1 cut(s) 364
BtsCI GGATG 1 cut(s) 391
BtsIMutI CAGTG 2 cut(s) 144, 472
CciI TCATGA 1 cut(s) 403
Csp6I GTAC 1 cut(s) 530
CviAII CATG 4 cut(s) 8, 335, 404, 557
CviJI RGCY 5 cut(s) 218, 270, 347, 367, 490
CviKI_1 RGCY 5 cut(s) 218, 270, 347, 367, 490
CviQI GTAC 1 cut(s) 530
DdeI CTNAG 2 cut(s) 271, 348
DpnI GATC 3 cut(s) 179, 426, 486
DpnII GATC 3 cut(s) 177, 424, 484
DraI TTTAAA 1 cut(s) 55
Eam1104I CTCTTC 2 cut(s) 55, 267
EarI CTCTTC 2 cut(s) 55, 267
Eco31I GGTCTC 1 cut(s) 223
Eco57I CTGAAG 1 cut(s) 105
EcoRII CCWGG 2 cut(s) 234, 381
EcoT22I ATGCAT 1 cut(s) 9
FaeI CATG 4 cut(s) 11, 338, 407, 560
FaiI YATR 7 cut(s) 9, 209, 297, 336, 405, 506, 558
FatI CATG 4 cut(s) 7, 334, 403, 556
Fnu4HI GCNGC 2 cut(s) 368, 378
FokI GGATG 1 cut(s) 398
Fsp4HI GCNGC 2 cut(s) 368, 378
GluI GCNGC 2 cut(s) 368, 378
Hin1II CATG 4 cut(s) 11, 338, 407, 560
HinfI GANTC 2 cut(s) 101, 257
Hpy188I TCNGA 3 cut(s) 124, 256, 274
Hpy188III TCNNGA 2 cut(s) 31, 404
HpyAV CCTTC 4 cut(s) 129, 177, 190, 485
HpyCH4III ACNGT 2 cut(s) 148, 467
HpyCH4V TGCA 2 cut(s) 7, 556
HpyF10VI GCNNNNNNNGC 1 cut(s) 386
HpyF3I CTNAG 2 cut(s) 271, 348
Hsp92II CATG 4 cut(s) 11, 338, 407, 560
Kzo9I GATC 3 cut(s) 177, 424, 484
LmnI GCTCC 2 cut(s) 364, 554
LpnPI CCDG 7 cut(s) 62, 221, 248, 368, 394, 395, 512
Lsp1109I GCAGC 1 cut(s) 364
LweI GCATC 1 cut(s) 376
MaeIII GTNAC 2 cut(s) 68, 439
MalI GATC 3 cut(s) 179, 426, 486
MboI GATC 3 cut(s) 177, 424, 484
MboII GAAGA 2 cut(s) 72, 254
MfeI CAATTG 1 cut(s) 134
MhlI GDGCHC 1 cut(s) 551
MluCI AATT 6 cut(s) 134, 285, 312, 340, 518, 539
MmeI TCCRAC 2 cut(s) 329, 518
MnlI CCTC 5 cut(s) 56, 118, 219, 343, 412
Mph1103I ATGCAT 1 cut(s) 9
MseI TTAA 3 cut(s) 54, 222, 246
MspCI CTTAAG 1 cut(s) 221
MspR9I CCNGG 2 cut(s) 236, 383
MunI CAATTG 1 cut(s) 134
MvaI CCWGG 2 cut(s) 236, 383
MwoI GCNNNNNNNGC 1 cut(s) 386
NdeII GATC 3 cut(s) 177, 424, 484
NlaIII CATG 4 cut(s) 11, 338, 407, 560
NlaIV GGNNCC 1 cut(s) 366
NsiI ATGCAT 1 cut(s) 9
NspI RCATGY 1 cut(s) 560
PagI TCATGA 1 cut(s) 403
PfeI GAWTC 2 cut(s) 101, 257
PflMI CCANNNNNTGG 1 cut(s) 500
PkrI GCNGC 2 cut(s) 369, 379
Ple19I CGATCG 1 cut(s) 427
Psp6I CCWGG 2 cut(s) 234, 381
PspGI CCWGG 2 cut(s) 234, 381
PspN4I GGNNCC 1 cut(s) 366
PvuI CGATCG 1 cut(s) 427
RsaI GTAC 1 cut(s) 531
RsaNI GTAC 1 cut(s) 530
SaqAI TTAA 3 cut(s) 54, 222, 246
SatI GCNGC 2 cut(s) 368, 378
Sau3AI GATC 3 cut(s) 177, 424, 484
ScrFI CCNGG 2 cut(s) 236, 383
SduI GDGCHC 1 cut(s) 551
SetI ASST 4 cut(s) 121, 252, 349, 492
SfaNI GCATC 1 cut(s) 376
SmlI CTYRAG 1 cut(s) 221
SmoI CTYRAG 1 cut(s) 221
Sse9I AATT 6 cut(s) 134, 285, 312, 340, 518, 539
SsiI CCGC 1 cut(s) 368
StyD4I CCNGG 2 cut(s) 234, 381
TaaI ACNGT 2 cut(s) 148, 467
TaqI TCGA 1 cut(s) 19
TasI AATT 6 cut(s) 134, 285, 312, 340, 518, 539
TauI GCSGC 1 cut(s) 370
TfiI GAWTC 2 cut(s) 101, 257
Tru1I TTAA 3 cut(s) 54, 222, 246
Tru9I TTAA 3 cut(s) 54, 222, 246
TscAI CASTG 2 cut(s) 151, 472
TseI GCWGC 1 cut(s) 377
TspDTI ATGAA 4 cut(s) 351, 372, 392, 531
TspRI CASTG 2 cut(s) 151, 472
Van91I CCANNNNNTGG 1 cut(s) 500
Vha464I CTTAAG 1 cut(s) 221
XapI RAATTY 2 cut(s) 312, 340
XceI RCATGY 1 cut(s) 560
Zsp2I ATGCAT 1 cut(s) 9
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.