pycom17g11090

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0007593
Physical Location & Seq
Reverse (-)
8627053 .. 8627800
748 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 591 bp
ATGCAAAAAGTCTCAATGTTATTAGTAATGAAGCTAATTGGAGCAAGATACTTCAACAAGGGCTTTGCTGCAGTTGCTGGCCCCCTCAACTCCTCCTTCAATTCGCCGCGCGACAACGATGGCCATGGTTCCCATACCTTGTCAACAGCTGGTGGAAATTTCGTAACCGGTGCAAGCGTGTTCGGCTTTGGTAATGGAACAGCAAAGGGTGGATCACCAAAATCCAGGGTTGCAGCCTATAAGGTCTGCTGGCCTCCAGTGGGAGGAGGTGGGTGCTTTGATGCAGATATATTGGCTGCATTTGATGTGGCCATCGATGACGGCGTTGATGTGTTGTCCGTTTCATTGGGTGGAGATCCCACCACATTCTTCAATGACAGTGTTTCAATTGGTGCTTTCCATGCTGTTAAGCGTGGCATTGTGGTGGTTTGCTCGGCGGGGAATTCGGGTCCAGCTGAAGGTACTGTCTCCAATATATCGCCATGGCAGATCACGGTCGGCGCCAGTACAATGGATAGGGAGTTTCCTAGTTATGTCACCCTTGGAAACTGGAAGCACTTGAAGGTTAGTTATAGTCCCTTTTTCCGCTAG

Protein Analysis

197

Amino Acids

20.32

Weight (kDa)

8.46

Isoelectric Point (pI)

23.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 34 - 172 2e-15 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 500
AccII CGCG 2 cut(s) 109, 111
AciI CCGC 3 cut(s) 107, 437, 586
AclWI GGATC 2 cut(s) 220, 350
AcoI YGGCCR 2 cut(s) 121, 309
AcsI RAATTY 2 cut(s) 157, 442
AcuI CTGAAG 1 cut(s) 477
AcyI GRCGYC 1 cut(s) 501
AfaI GTAC 2 cut(s) 463, 508
AfiI CCNNNNNNNGG 3 cut(s) 260, 263, 458
AgeI ACCGGT 1 cut(s) 167
AgsI TTSAA 5 cut(s) 55, 100, 373, 387, 562
AjnI CCWGG 1 cut(s) 224
AluBI AGCT 3 cut(s) 34, 149, 455
AluI AGCT 3 cut(s) 34, 149, 455
Alw26I GTCTC 2 cut(s) 16, 472
AlwI GGATC 2 cut(s) 220, 350
AlwNI CAGNNNCTG 1 cut(s) 77
AoxI GGCC 4 cut(s) 79, 121, 251, 309
ApeKI GCWGC 3 cut(s) 68, 233, 296
ApoI RAATTY 2 cut(s) 157, 442
AsiGI ACCGGT 1 cut(s) 167
AspLEI GCGC 2 cut(s) 111, 503
AspS9I GGNCC 2 cut(s) 80, 449
AsuHPI GGTGA 2 cut(s) 207, 529
AvaII GGWCC 1 cut(s) 449
BalI TGGCCA 2 cut(s) 123, 311
BanI GGYRCC 1 cut(s) 500
BbvI GCAGC 3 cut(s) 55, 245, 283
BccI CCATC 2 cut(s) 113, 320
BceAI ACGGC 1 cut(s) 337
BciT130I CCWGG 1 cut(s) 226
BcoDI GTCTC 2 cut(s) 16, 472
BfaI CTAG 2 cut(s) 528, 589
BfmI CTRYAG 1 cut(s) 69
BfoI RGCGCY 1 cut(s) 504
BisI GCNGC 4 cut(s) 69, 107, 234, 297
BlsI GCNGC 4 cut(s) 70, 108, 235, 298
Bme1390I CCNGG 1 cut(s) 226
Bme18I GGWCC 1 cut(s) 449
BmgT120I GGNCC 2 cut(s) 80, 449
BmiI GGNNCC 4 cut(s) 82, 130, 450, 502
BmrFI CCNGG 1 cut(s) 226
BmsI GCATC 1 cut(s) 271
BpmI CTGGAG 1 cut(s) 240
Bsa29I ATCGAT 1 cut(s) 315
BsaHI GRCGYC 1 cut(s) 501
BsaJI CCNNGG 4 cut(s) 124, 225, 482, 541
BsaWI WCCGGW 1 cut(s) 167
Bsc4I CCNNNNNNNGG 3 cut(s) 260, 263, 458
Bse118I RCCGGY 1 cut(s) 167
Bse1I ACTGG 3 cut(s) 257, 504, 554
BseBI CCWGG 1 cut(s) 226
BseCI ATCGAT 1 cut(s) 315
BseDI CCNNGG 4 cut(s) 124, 225, 482, 541
BseLI CCNNNNNNNGG 3 cut(s) 260, 263, 458
BseNI ACTGG 3 cut(s) 257, 504, 554
BseRI GAGGAG 2 cut(s) 82, 279
BseXI GCAGC 3 cut(s) 55, 245, 283
Bsh1236I CGCG 2 cut(s) 109, 111
Bsh1285I CGRYCG 1 cut(s) 498
BshFI GGCC 4 cut(s) 81, 123, 253, 311
BshNI GGYRCC 1 cut(s) 500
BshTI ACCGGT 1 cut(s) 167
BshVI ATCGAT 1 cut(s) 315
BsiEI CGRYCG 1 cut(s) 498
BsiSI CCGG 1 cut(s) 168
BslFI GGGAC 1 cut(s) 561
BslI CCNNNNNNNGG 3 cut(s) 260, 263, 458
BsmAI GTCTC 2 cut(s) 16, 472
BsmFI GGGAC 1 cut(s) 561
BsnI GGCC 4 cut(s) 81, 123, 253, 311
Bsp143I GATC 3 cut(s) 212, 355, 489
Bsp19I CCATGG 2 cut(s) 124, 482
BspACI CCGC 3 cut(s) 107, 437, 586
BspANI GGCC 4 cut(s) 81, 123, 253, 311
BspDI ATCGAT 1 cut(s) 315
BspFNI CGCG 2 cut(s) 109, 111
BspLI GGNNCC 4 cut(s) 82, 130, 450, 502
BspMAI CTGCAG 1 cut(s) 73
BspPI GGATC 2 cut(s) 220, 350
BspT107I GGYRCC 1 cut(s) 500
BsrFI RCCGGY 1 cut(s) 167
BsrI ACTGG 3 cut(s) 257, 504, 554
BssAI RCCGGY 1 cut(s) 167
BssECI CCNNGG 4 cut(s) 124, 225, 482, 541
BssMI GATC 3 cut(s) 212, 355, 489
BssNI GRCGYC 1 cut(s) 501
BssT1I CCWWGG 3 cut(s) 124, 482, 541
Bst2UI CCWGG 1 cut(s) 226
Bst4CI ACNGT 3 cut(s) 380, 466, 496
BstACI GRCGYC 1 cut(s) 501
BstC8I GCNNGC 3 cut(s) 79, 175, 251
BstDSI CCRYGG 2 cut(s) 124, 482
BstFNI CGCG 2 cut(s) 109, 111
BstH2I RGCGCY 1 cut(s) 504
BstHHI GCGC 2 cut(s) 111, 503
BstKTI GATC 3 cut(s) 215, 358, 492
BstMAI GTCTC 2 cut(s) 16, 472
BstMBI GATC 3 cut(s) 212, 355, 489
BstMCI CGRYCG 1 cut(s) 498
BstMWI GCNNNNNNNGC 3 cut(s) 74, 183, 401
BstNI CCWGG 1 cut(s) 226
BstSCI CCNGG 1 cut(s) 224
BstSFI CTRYAG 1 cut(s) 69
BstUI CGCG 2 cut(s) 109, 111
BstV1I GCAGC 3 cut(s) 55, 245, 283
BstX2I RGATCY 1 cut(s) 355
BstXI CCANNNNNNTGG 1 cut(s) 511
BstYI RGATCY 1 cut(s) 355
Bsu15I ATCGAT 1 cut(s) 315
BsuRI GGCC 4 cut(s) 81, 123, 253, 311
BsuTUI ATCGAT 1 cut(s) 315
BtgI CCRYGG 2 cut(s) 124, 482
BtsIMutI CAGTG 2 cut(s) 264, 385
Cac8I GCNNGC 3 cut(s) 79, 175, 251
CaiI CAGNNNCTG 1 cut(s) 77
CfoI GCGC 2 cut(s) 111, 503
Cfr10I RCCGGY 1 cut(s) 167
Cfr13I GGNCC 2 cut(s) 80, 449
ClaI ATCGAT 1 cut(s) 315
Csp6I GTAC 2 cut(s) 462, 507
CspAI ACCGGT 1 cut(s) 167
CviAII CATG 3 cut(s) 125, 401, 483
CviQI GTAC 2 cut(s) 462, 507
DinI GGCGCC 1 cut(s) 502
DpnI GATC 3 cut(s) 214, 357, 491
DpnII GATC 3 cut(s) 212, 355, 489
EaeI YGGCCR 2 cut(s) 121, 309
Eco130I CCWWGG 3 cut(s) 124, 482, 541
Eco47I GGWCC 1 cut(s) 449
Eco57I CTGAAG 1 cut(s) 477
EcoRI GAATTC 1 cut(s) 442
EcoRII CCWGG 1 cut(s) 224
EcoT14I CCWWGG 3 cut(s) 124, 482, 541
EgeI GGCGCC 1 cut(s) 502
EheI GGCGCC 1 cut(s) 502
ErhI CCWWGG 3 cut(s) 124, 482, 541
FaeI CATG 3 cut(s) 128, 404, 486
FaiI YATR 9 cut(s) 126, 135, 240, 290, 402, 476, 484, 534, 573
FaqI GGGAC 1 cut(s) 561
FatI CATG 3 cut(s) 124, 400, 482
FauI CCCGC 1 cut(s) 430
Fnu4HI GCNGC 4 cut(s) 69, 107, 234, 297
Fsp4HI GCNGC 4 cut(s) 69, 107, 234, 297
FspBI CTAG 2 cut(s) 528, 589
GlaI GCGC 2 cut(s) 110, 502
GluI GCNGC 4 cut(s) 69, 107, 234, 297
GsuI CTGGAG 1 cut(s) 240
HaeII RGCGCY 1 cut(s) 504
HaeIII GGCC 4 cut(s) 81, 123, 253, 311
HapII CCGG 1 cut(s) 168
HhaI GCGC 2 cut(s) 111, 503
Hin1I GRCGYC 1 cut(s) 501
Hin1II CATG 3 cut(s) 128, 404, 486
Hin6I GCGC 2 cut(s) 109, 501
HinP1I GCGC 2 cut(s) 109, 501
HincII GTYRAC 1 cut(s) 144
HindII GTYRAC 1 cut(s) 144
HpaII CCGG 1 cut(s) 168
HphI GGTGA 2 cut(s) 207, 529
Hpy166II GTNNAC 1 cut(s) 144
Hpy8I GTNNAC 1 cut(s) 144
HpyAV CCTTC 3 cut(s) 106, 452, 556
HpyCH4III ACNGT 3 cut(s) 380, 466, 496
HpyCH4V TGCA 6 cut(s) 4, 71, 173, 233, 284, 299
HpyF10VI GCNNNNNNNGC 3 cut(s) 74, 183, 401
Hsp92I GRCGYC 1 cut(s) 501
Hsp92II CATG 3 cut(s) 128, 404, 486
HspAI GCGC 2 cut(s) 109, 501
KasI GGCGCC 1 cut(s) 500
Kzo9I GATC 3 cut(s) 212, 355, 489
LmnI GCTCC 1 cut(s) 41
Lsp1109I GCAGC 3 cut(s) 55, 245, 283
LweI GCATC 1 cut(s) 271
MaeI CTAG 2 cut(s) 528, 589
MaeIII GTNAC 2 cut(s) 163, 535
MalI GATC 3 cut(s) 214, 357, 491
MboI GATC 3 cut(s) 212, 355, 489
MboII GAAGA 1 cut(s) 361
MfeI CAATTG 1 cut(s) 387
MflI RGATCY 1 cut(s) 355
MlsI TGGCCA 2 cut(s) 123, 311
MluCI AATT 5 cut(s) 36, 100, 157, 387, 442
MluNI TGGCCA 2 cut(s) 123, 311
Mly113I GGCGCC 1 cut(s) 501
MnlI CCTC 5 cut(s) 95, 103, 257, 260, 264
Mox20I TGGCCA 2 cut(s) 123, 311
MscI TGGCCA 2 cut(s) 123, 311
MseI TTAA 1 cut(s) 408
MslI CAYNNNNRTG 1 cut(s) 422
Msp20I TGGCCA 2 cut(s) 123, 311
MspA1I CMGCKG 2 cut(s) 149, 455
MspI CCGG 1 cut(s) 168
MspR9I CCNGG 1 cut(s) 226
MunI CAATTG 1 cut(s) 387
MvaI CCWGG 1 cut(s) 226
MvnI CGCG 2 cut(s) 109, 111
MwoI GCNNNNNNNGC 3 cut(s) 74, 183, 401
NarI GGCGCC 1 cut(s) 501
NcoI CCATGG 2 cut(s) 124, 482
NdeII GATC 3 cut(s) 212, 355, 489
NlaIII CATG 3 cut(s) 128, 404, 486
NlaIV GGNNCC 4 cut(s) 82, 130, 450, 502
NmeAIII GCCGAG 1 cut(s) 413
NmuCI GTSAC 1 cut(s) 535
PcsI WCGNNNNNNNCGW 1 cut(s) 321
PinAI ACCGGT 1 cut(s) 167
PkrI GCNGC 4 cut(s) 70, 108, 235, 298
PluTI GGCGCC 1 cut(s) 504
Psp6I CCWGG 1 cut(s) 224
PspGI CCWGG 1 cut(s) 224
PspN4I GGNNCC 4 cut(s) 82, 130, 450, 502
PspPI GGNCC 2 cut(s) 80, 449
PstI CTGCAG 1 cut(s) 73
PstNI CAGNNNCTG 1 cut(s) 77
PsuI RGATCY 1 cut(s) 355
PvuII CAGCTG 2 cut(s) 149, 455
RsaI GTAC 2 cut(s) 463, 508
RsaNI GTAC 2 cut(s) 462, 507
RseI CAYNNNNRTG 1 cut(s) 422
SaqAI TTAA 1 cut(s) 408
SatI GCNGC 4 cut(s) 69, 107, 234, 297
Sau3AI GATC 3 cut(s) 212, 355, 489
Sau96I GGNCC 2 cut(s) 80, 449
ScrFI CCNGG 1 cut(s) 226
SetI ASST 8 cut(s) 36, 140, 151, 246, 271, 457, 463, 567
SfaNI GCATC 1 cut(s) 271
SfcI CTRYAG 1 cut(s) 69
SfoI GGCGCC 1 cut(s) 502
SinI GGWCC 1 cut(s) 449
SmiMI CAYNNNNRTG 1 cut(s) 422
Sse9I AATT 5 cut(s) 36, 100, 157, 387, 442
SsiI CCGC 3 cut(s) 107, 437, 586
SspDI GGCGCC 1 cut(s) 500
SspMI CTAG 2 cut(s) 528, 589
StyD4I CCNGG 1 cut(s) 224
StyI CCWWGG 3 cut(s) 124, 482, 541
TaaI ACNGT 3 cut(s) 380, 466, 496
TaqI TCGA 1 cut(s) 315
TasI AATT 5 cut(s) 36, 100, 157, 387, 442
TatI WGTACW 1 cut(s) 506
TauI GCSGC 1 cut(s) 109
Tru1I TTAA 1 cut(s) 408
Tru9I TTAA 1 cut(s) 408
TscAI CASTG 2 cut(s) 264, 385
TseFI GTSAC 1 cut(s) 535
TseI GCWGC 3 cut(s) 68, 233, 296
Tsp45I GTSAC 1 cut(s) 535
TspDTI ATGAA 2 cut(s) 44, 333
TspGWI ACGGA 1 cut(s) 328
TspRI CASTG 2 cut(s) 264, 385
VpaK11BI GGWCC 1 cut(s) 449
XapI RAATTY 2 cut(s) 157, 442
XspI CTAG 2 cut(s) 528, 589
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.