pycom17g13530

serine threonine-protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0007469
Physical Location & Seq
Forward (+)
11160116 .. 11167487
7372 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1449 bp
ATGGAAACTGATAATGCTAATGTTAAGCAAGCTGAAGATGCTAAACTCCTTGCCAATGAAGCATATAAAGCTCATAAGTTTGCTCAAGCTATTGATCTGTATACACAAGCGATTGAATTAAACAGTCAGAATGCTGTATACTGGGCCAATCGTTCAATTGCCCACCTTAAACTGGAGGAATATGGTAGCGCCATACAAGATGCTTCCAAGGCTATTGAAATCGATCCCAAATATTCAAAGGGATACTATAGGCGAGGTGCAGCTTATCTTGCTATGGGGAAATTTAAAGAAGCGTTAAAGGATTTTCAGCAGGTCAAAAGAATCTGTCCAAATGATCCTGATGCTACCAAGAAATTGAAGGAATGTGAGAAAGCAGTGATGAAACTTAAATTTGCTGAAGCAATATCTGTACCGGAGGCTGAAAGGCGTCCTGTAGCTGATTCTATTAATTATCATTCTATAGATGTGGAGCCACAATATGGTGGTGCAAGGATAGAAGGGGACGTTGTTACTTTGGATTTTGTGAAGAAAATGATGGAGGATTTCAAGAGTCAGAAGAATTTACACCAACGATATGCGTTCCAAATTGTTTTACAGACGAAAGAAATGTTGCAAGCCATGCCTTCTCTGGTTGATATAAACATTCCTGAGGGCAAACATTTTACTGTTTGTGGTGACGTACATGGTCAGTTCTATGATCTTCTAAATATTTTTGAGCTTAACGGGCTTCCTTCAGATGAGAATCCATATCTATTCAATGGAGACTTTGTGGACAGGGGATCTTTTTCTTTGGAGGTGATCCTAACGTTGTTTGCATTTAAGTGCATGTCTCCATCAGCTATATATCTCGCAAGAGGAAACCATGAGAGCAAGAGCATGAATAGGATATATGGTTTTGAGGGCGAAGTCCAGTCCAAATTAAGTGAAAAGTTTGTGGAGCTGTTTGCTGAAGTGTTTTGTTGTCTACCGCTGGCTCATGTGATCAATGGGAAGGTTTTTGTAGTCCATGGGGGTCTTTTCAGTGTTGATGGAGTGAAACTCAGTGACATTAGAGCAATTAATCGGTTTTGTGAACCTCCTGAGGAAGGGTTAATGTGTGAATTGCTGTGGAGTGATCCACAACCTGCACCTGGAAGGGGGCCAAGCAAACGAGGCGTAGGTCTTTCTTTTGGTGGTGATGTGACAAAAAGATTTTTGCAGGAAAACAATTTAGTTAGTTGTGCGATCGATCATGAAGTAAAGGACGAGGGTTATGAGATTGAGCATGATGGCAAACTCATCACTGTTTTTTCTGCTCCAAATTACTGTGATCAGATGGGTAACAAGGGTGCCTTTATCCGTTTCGAATCACCAGAATTGAAGCCTAACATTGTTAATTTCTCAGCAGTGGCACATCCTGACGTCAAGCCAATGGCATATGCAAACAACTTCCTCCGAATGTTTCAGTAA

Protein Analysis

483

Amino Acids

54.17

Weight (kDa)

5.66

Isoelectric Point (pI)

37.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_BSK1_C PF25575 7 - 83 1.7e-08 Serine/threonine-protein kinase BSK1-like, TPR repeats
TPR_16 PF13432 17 - 78 5.7e-07 Tetratricopeptide repeat
TPR_1 PF00515 80 - 112 3.6e-09 Tetratricopeptide repeat
TPR_2 PF07719 80 - 112 3.8e-07 Tetratricopeptide repeat
PPP5 PF08321 119 - 212 2.4e-28 PPP5 TPR repeat region
Metallophos PF00149 220 - 404 9.1e-34 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1404
Acc36I ACCTGC 2 cut(s) 301, 1132
AccB1I GGYRCC 1 cut(s) 1328
AccB7I CCANNNNNTGG 1 cut(s) 479
AccI GTMKAC 3 cut(s) 101, 138, 964
AciI CCGC 1 cut(s) 968
AclI AACGTT 1 cut(s) 806
AclWI GGATC 5 cut(s) 218, 329, 787, 793, 1109
AcsI RAATTY 3 cut(s) 281, 389, 559
AcuI CTGAAG 4 cut(s) 54, 417, 717, 969
AcyI GRCGYC 2 cut(s) 427, 1401
AfaI GTAC 2 cut(s) 411, 681
AfiI CCNNNNNNNGG 5 cut(s) 172, 479, 1085, 1130, 1136
AgsI TTSAA 8 cut(s) 116, 156, 218, 237, 358, 547, 757, 1360
AjnI CCWGG 1 cut(s) 1129
AluBI AGCT 8 cut(s) 32, 71, 89, 263, 437, 718, 839, 940
AluI AGCT 8 cut(s) 32, 71, 89, 263, 437, 718, 839, 940
Alw26I GTCTC 2 cut(s) 756, 834
AlwI GGATC 5 cut(s) 218, 329, 787, 793, 1109
AoxI GGCC 2 cut(s) 144, 1139
ApeKI GCWGC 1 cut(s) 260
ApoI RAATTY 3 cut(s) 281, 389, 559
AseI ATTAAT 2 cut(s) 447, 1059
AspLEI GCGC 1 cut(s) 191
AspS9I GGNCC 2 cut(s) 144, 1139
AsuHPI GGTGA 4 cut(s) 686, 808, 1187, 1341
AsuII TTCGAA 1 cut(s) 1344
AxyI CCTNAGG 2 cut(s) 648, 1080
BanI GGYRCC 1 cut(s) 1328
BbvI GCAGC 1 cut(s) 272
BccI CCATC 5 cut(s) 529, 841, 1022, 1262, 1309
BciT130I CCWGG 1 cut(s) 1131
BciVI GTATCC 1 cut(s) 236
BclI TGATCA 2 cut(s) 981, 1309
BcoDI GTCTC 2 cut(s) 756, 834
BfmI CTRYAG 3 cut(s) 247, 432, 459
BfoI RGCGCY 1 cut(s) 192
BfuAI ACCTGC 2 cut(s) 301, 1132
BfuI GTATCC 1 cut(s) 236
BisI GCNGC 1 cut(s) 261
BlsI GCNGC 1 cut(s) 262
Bme1390I CCNGG 1 cut(s) 1131
BmgT120I GGNCC 2 cut(s) 144, 1139
BmiI GGNNCC 3 cut(s) 471, 1140, 1330
BmrFI CCNGG 1 cut(s) 1131
BmrI ACTGGG 1 cut(s) 151
BmsI GCATC 3 cut(s) 28, 190, 331
BmuI ACTGGG 1 cut(s) 151
BplI GAGNNNNNCTC 2 cut(s) 1023, 1055
BpmI CTGGAG 1 cut(s) 194
Bpu14I TTCGAA 1 cut(s) 1344
BpuEI CTTGAG 1 cut(s) 69
Bsa29I ATCGAT 2 cut(s) 222, 1227
BsaBI GATNNNNATC 1 cut(s) 741
BsaHI GRCGYC 2 cut(s) 427, 1401
BsaJI CCNNGG 2 cut(s) 207, 1006
BsaWI WCCGGW 1 cut(s) 412
BsaXI ACNNNNNCTCC 2 cut(s) 753, 783
Bsc4I CCNNNNNNNGG 5 cut(s) 172, 479, 1085, 1130, 1136
Bse1I ACTGG 3 cut(s) 146, 177, 910
Bse21I CCTNAGG 2 cut(s) 648, 1080
Bse8I GATNNNNATC 1 cut(s) 741
BseBI CCWGG 1 cut(s) 1131
BseCI ATCGAT 2 cut(s) 222, 1227
BseDI CCNNGG 2 cut(s) 207, 1006
BseGI GGATG 1 cut(s) 1393
BseJI GATNNNNATC 1 cut(s) 741
BseLI CCNNNNNNNGG 5 cut(s) 172, 479, 1085, 1130, 1136
BseMII CTCAG 4 cut(s) 639, 1054, 1071, 1395
BseNI ACTGG 3 cut(s) 146, 177, 910
BseXI GCAGC 1 cut(s) 272
BsgI GTGCAG 2 cut(s) 279, 1110
Bsh1285I CGRYCG 1 cut(s) 1227
BshFI GGCC 2 cut(s) 146, 1141
BshNI GGYRCC 1 cut(s) 1328
BshVI ATCGAT 2 cut(s) 222, 1227
BsiEI CGRYCG 1 cut(s) 1227
BsiSI CCGG 1 cut(s) 413
BslFI GGGAC 1 cut(s) 515
BslI CCNNNNNNNGG 5 cut(s) 172, 479, 1085, 1130, 1136
BsmAI GTCTC 2 cut(s) 756, 834
BsmFI GGGAC 1 cut(s) 515
BsmI GAATGC 1 cut(s) 136
BsnI GGCC 2 cut(s) 146, 1141
Bsp119I TTCGAA 1 cut(s) 1344
Bsp19I CCATGG 1 cut(s) 1006
BspACI CCGC 1 cut(s) 968
BspANI GGCC 2 cut(s) 146, 1141
BspCNI CTCAG 4 cut(s) 640, 1053, 1072, 1394
BspDI ATCGAT 2 cut(s) 222, 1227
BspHI TCATGA 1 cut(s) 1231
BspLI GGNNCC 3 cut(s) 471, 1140, 1330
BspMI ACCTGC 2 cut(s) 301, 1132
BspPI GGATC 5 cut(s) 218, 329, 787, 793, 1109
BspT104I TTCGAA 1 cut(s) 1344
BspT107I GGYRCC 1 cut(s) 1328
BsrI ACTGG 3 cut(s) 146, 177, 910
BssECI CCNNGG 2 cut(s) 207, 1006
BssNAI GTATAC 2 cut(s) 102, 139
BssNI GRCGYC 2 cut(s) 427, 1401
BssT1I CCWWGG 2 cut(s) 207, 1006
Bst1107I GTATAC 2 cut(s) 102, 139
Bst2UI CCWGG 1 cut(s) 1131
Bst4CI ACNGT 4 cut(s) 125, 667, 1285, 1307
BstACI GRCGYC 2 cut(s) 427, 1401
BstAPI GCANNNNNTGC 1 cut(s) 619
BstBI TTCGAA 1 cut(s) 1344
BstC8I GCNNGC 3 cut(s) 30, 615, 972
BstDEI CTNAG 4 cut(s) 648, 1040, 1080, 1381
BstDSI CCRYGG 1 cut(s) 1006
BstENI CCTNNNNNAGG 1 cut(s) 1083
BstF5I GGATG 1 cut(s) 1393
BstH2I RGCGCY 1 cut(s) 192
BstHHI GCGC 1 cut(s) 191
BstMAI GTCTC 2 cut(s) 756, 834
BstMCI CGRYCG 1 cut(s) 1227
BstMWI GCNNNNNNNGC 8 cut(s) 38, 59, 68, 209, 269, 619, 724, 1152
BstNI CCWGG 1 cut(s) 1131
BstNSI RCATGY 1 cut(s) 829
BstSCI CCNGG 1 cut(s) 1129
BstSFI CTRYAG 3 cut(s) 247, 432, 459
BstV1I GCAGC 1 cut(s) 272
BstX2I RGATCY 1 cut(s) 779
BstYI RGATCY 1 cut(s) 779
BstZ17I GTATAC 2 cut(s) 102, 139
Bsu15I ATCGAT 2 cut(s) 222, 1227
Bsu36I CCTNAGG 2 cut(s) 648, 1080
BsuI GTATCC 1 cut(s) 236
BsuRI GGCC 2 cut(s) 146, 1141
BsuTUI ATCGAT 2 cut(s) 222, 1227
BtgI CCRYGG 1 cut(s) 1006
BtsCI GGATG 1 cut(s) 1393
BtsI GCAGTG 2 cut(s) 381, 1392
BtsIMutI CAGTG 5 cut(s) 381, 1027, 1048, 1281, 1392
BveI ACCTGC 2 cut(s) 301, 1132
Cac8I GCNNGC 3 cut(s) 30, 615, 972
CciI TCATGA 1 cut(s) 1231
CfoI GCGC 1 cut(s) 191
Cfr13I GGNCC 2 cut(s) 144, 1139
ClaI ATCGAT 2 cut(s) 222, 1227
CseI GACGC 1 cut(s) 416
Csp6I GTAC 2 cut(s) 410, 680
CviAII CATG 9 cut(s) 619, 683, 826, 863, 877, 977, 1007, 1232, 1265
CviQI GTAC 2 cut(s) 410, 680
DdeI CTNAG 4 cut(s) 648, 1040, 1080, 1381
DraI TTTAAA 1 cut(s) 286
Eco130I CCWWGG 2 cut(s) 207, 1006
Eco57I CTGAAG 4 cut(s) 54, 417, 717, 969
Eco81I CCTNAGG 2 cut(s) 648, 1080
EcoNI CCTNNNNNAGG 1 cut(s) 1083
EcoRII CCWGG 1 cut(s) 1129
EcoT14I CCWWGG 2 cut(s) 207, 1006
ErhI CCWWGG 2 cut(s) 207, 1006
FaeI CATG 9 cut(s) 622, 686, 829, 866, 880, 980, 1010, 1235, 1268
FalI AAGNNNNNCTT 2 cut(s) 1316, 1348
FaqI GGGAC 1 cut(s) 515
FatI CATG 9 cut(s) 618, 682, 825, 862, 876, 976, 1006, 1231, 1264
FauNDI CATATG 1 cut(s) 1417
FbaI TGATCA 2 cut(s) 981, 1309
FblI GTMKAC 3 cut(s) 101, 138, 964
Fnu4HI GCNGC 1 cut(s) 261
FokI GGATG 1 cut(s) 1380
Fsp4HI GCNGC 1 cut(s) 261
GlaI GCGC 1 cut(s) 190
GluI GCNGC 1 cut(s) 261
GsuI CTGGAG 1 cut(s) 194
HaeII RGCGCY 1 cut(s) 192
HaeIII GGCC 2 cut(s) 146, 1141
HapII CCGG 1 cut(s) 413
HgaI GACGC 1 cut(s) 416
HhaI GCGC 1 cut(s) 191
Hin1I GRCGYC 2 cut(s) 427, 1401
Hin1II CATG 9 cut(s) 622, 686, 829, 866, 880, 980, 1010, 1235, 1268
Hin6I GCGC 1 cut(s) 189
HinP1I GCGC 1 cut(s) 189
HinfI GANTC 5 cut(s) 321, 440, 550, 742, 1346
HpaII CCGG 1 cut(s) 413
HphI GGTGA 4 cut(s) 686, 808, 1187, 1341
Hpy166II GTNNAC 5 cut(s) 102, 139, 772, 965, 1073
Hpy188I TCNGA 5 cut(s) 129, 555, 736, 1314, 1436
Hpy188III TCNNGA 6 cut(s) 338, 547, 647, 1079, 1232, 1397
Hpy8I GTNNAC 5 cut(s) 102, 139, 772, 965, 1073
HpyAV CCTTC 7 cut(s) 352, 491, 633, 741, 985, 1079, 1128
HpyCH4III ACNGT 4 cut(s) 125, 667, 1285, 1307
HpyCH4IV ACGT 4 cut(s) 504, 678, 806, 1401
HpyCH4V TGCA 8 cut(s) 260, 488, 613, 815, 825, 1127, 1198, 1421
HpyF10VI GCNNNNNNNGC 8 cut(s) 38, 59, 68, 209, 269, 619, 724, 1152
HpyF3I CTNAG 4 cut(s) 648, 1040, 1080, 1381
HpySE526I ACGT 4 cut(s) 504, 678, 806, 1401
Hsp92I GRCGYC 2 cut(s) 427, 1401
Hsp92II CATG 9 cut(s) 622, 686, 829, 866, 880, 980, 1010, 1235, 1268
HspAI GCGC 1 cut(s) 189
Ksp22I TGATCA 2 cut(s) 981, 1309
LmnI GCTCC 3 cut(s) 469, 937, 1300
Lsp1109I GCAGC 1 cut(s) 272
LweI GCATC 3 cut(s) 28, 190, 331
MaeII ACGT 4 cut(s) 504, 678, 806, 1401
MaeIII GTNAC 5 cut(s) 508, 674, 1043, 1180, 1319
MboII GAAGA 4 cut(s) 47, 538, 568, 692
MfeI CAATTG 1 cut(s) 156
MflI RGATCY 1 cut(s) 779
MlyI GAGTC 1 cut(s) 559
MslI CAYNNNNRTG 1 cut(s) 820
MspA1I CMGCKG 1 cut(s) 970
MspI CCGG 1 cut(s) 413
MspR9I CCNGG 1 cut(s) 1131
MunI CAATTG 1 cut(s) 156
Mva1269I GAATGC 1 cut(s) 136
MvaI CCWGG 1 cut(s) 1131
MwoI GCNNNNNNNGC 8 cut(s) 38, 59, 68, 209, 269, 619, 724, 1152
NcoI CCATGG 1 cut(s) 1006
NdeI CATATG 1 cut(s) 1417
NlaIII CATG 9 cut(s) 622, 686, 829, 866, 880, 980, 1010, 1235, 1268
NlaIV GGNNCC 3 cut(s) 471, 1140, 1330
NmuCI GTSAC 3 cut(s) 674, 1043, 1180
NspI RCATGY 1 cut(s) 829
NspV TTCGAA 1 cut(s) 1344
PagI TCATGA 1 cut(s) 1231
PctI GAATGC 1 cut(s) 136
PfeI GAWTC 4 cut(s) 321, 440, 742, 1346
PflMI CCANNNNNTGG 1 cut(s) 479
PkrI GCNGC 1 cut(s) 262
Ple19I CGATCG 1 cut(s) 1227
PleI GAGTC 1 cut(s) 558
PpsI GAGTC 1 cut(s) 558
PshBI ATTAAT 2 cut(s) 447, 1059
Psp1406I AACGTT 1 cut(s) 806
Psp6I CCWGG 1 cut(s) 1129
PspGI CCWGG 1 cut(s) 1129
PspN4I GGNNCC 3 cut(s) 471, 1140, 1330
PspPI GGNCC 2 cut(s) 144, 1139
PsuI RGATCY 1 cut(s) 779
PvuI CGATCG 1 cut(s) 1227
RsaI GTAC 2 cut(s) 411, 681
RsaNI GTAC 2 cut(s) 410, 680
RseI CAYNNNNRTG 1 cut(s) 820
SatI GCNGC 1 cut(s) 261
Sau96I GGNCC 2 cut(s) 144, 1139
SchI GAGTC 1 cut(s) 559
ScrFI CCNGG 1 cut(s) 1131
SfaNI GCATC 3 cut(s) 28, 190, 331
SfcI CTRYAG 3 cut(s) 247, 432, 459
SfuI TTCGAA 1 cut(s) 1344
SmiMI CAYNNNNRTG 1 cut(s) 820
SmlI CTYRAG 1 cut(s) 84
SmoI CTYRAG 1 cut(s) 84
SsiI CCGC 1 cut(s) 968
SspI AATATT 2 cut(s) 233, 709
StyD4I CCNGG 1 cut(s) 1129
StyI CCWWGG 2 cut(s) 207, 1006
TaaI ACNGT 4 cut(s) 125, 667, 1285, 1307
TaiI ACGT 4 cut(s) 507, 681, 809, 1404
TaqI TCGA 3 cut(s) 222, 1227, 1344
TfiI GAWTC 4 cut(s) 321, 440, 742, 1346
TscAI CASTG 5 cut(s) 381, 1027, 1048, 1288, 1392
TseFI GTSAC 3 cut(s) 674, 1043, 1180
TseI GCWGC 1 cut(s) 260
Tsp45I GTSAC 3 cut(s) 674, 1043, 1180
TspDTI ATGAA 4 cut(s) 72, 395, 893, 1248
TspGWI ACGGA 1 cut(s) 1328
TspRI CASTG 5 cut(s) 381, 1027, 1048, 1288, 1392
Van91I CCANNNNNTGG 1 cut(s) 479
VspI ATTAAT 2 cut(s) 447, 1059
XagI CCTNNNNNAGG 1 cut(s) 1083
XapI RAATTY 3 cut(s) 281, 389, 559
XceI RCATGY 1 cut(s) 829
XcmI CCANNNNNNNNNTGG 1 cut(s) 625
XmiI GTMKAC 3 cut(s) 101, 138, 964
ZraI GACGTC 1 cut(s) 1402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.