pycom17g16850

peptidyl-tRNA hydrolase 2, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0006608
Physical Location & Seq
Reverse (-)
14826369 .. 14828211
1843 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 585 bp
ATGATGATGGGGTTGGAGTTGGGGTTGGGGTGGGTGGGTGCGATTTTGGTAGGAGCGGGCTGGCTTGCTTTGGGTTATTGCTTTGGCGCTCGTTACCCTCCTTCTCGCATTATTTTCTCAGCCAGACTCGCTAAGCAGGCTGCCAATGATCGATCGTTAACAATGGAAAGAAGAAGAAGAACAAGGACAAGCCCAAAGATCCCCTCGAGATTGAAAACCTCGCCGACATTCTCCAAGATTTCAAAATGGTTTTGGTGGTCAGGAATCGATCTAAAGATGGGTAAAGGGAAAATTGCCGCTCAATGCAGCCATGCGACTTTAGGCCTCTATAAAAAGGTCCTCCATCGAGCACCAAAAGCTTTAAACAGGTGGGAGATGTGTGCACAGCCTAAAGTTGTTGTGAAAATAGAAAGTGAAGAAGATATGCTACTTTTGCAAGAAAGGGCTAAATTGCTGAACTTACCGACACACATTACAATTGATGCTGGCAGGACTCAGATTGCACCAAATTCAAGGACAGTGATGGCTATTCTTGGACCTGTTGAAGTGGTTGATGATGTAACAGGTGTACTGAAGCTCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTH2

Protein Analysis

195

Amino Acids

21.6

Weight (kDa)

10.29

Isoelectric Point (pI)

46.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTH2 PF01981 90 - 194 2.3e-35 Peptidyl-tRNA hydrolase PTH2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 56, 299
AciI CCGC 2 cut(s) 56, 297
AclWI GGATC 1 cut(s) 193
AcsI RAATTY 1 cut(s) 508
AfaI GTAC 1 cut(s) 570
AgsI TTSAA 4 cut(s) 214, 243, 513, 545
AluBI AGCT 2 cut(s) 359, 577
AluI AGCT 2 cut(s) 359, 577
Alw21I GWGCWC 2 cut(s) 352, 385
Alw44I GTGCAC 1 cut(s) 381
AlwI GGATC 1 cut(s) 193
Ama87I CYCGRG 1 cut(s) 205
AoxI GGCC 1 cut(s) 322
ApaLI GTGCAC 1 cut(s) 381
ApeKI GCWGC 2 cut(s) 140, 306
ApoI RAATTY 1 cut(s) 508
AspLEI GCGC 1 cut(s) 89
AspS9I GGNCC 2 cut(s) 337, 536
AvaI CYCGRG 1 cut(s) 205
AvaII GGWCC 2 cut(s) 337, 536
BaeGI GKGCMC 1 cut(s) 385
BarI GAAGNNNNNNTAC 2 cut(s) 411, 443
Bbv12I GWGCWC 2 cut(s) 352, 385
BbvI GCAGC 2 cut(s) 127, 318
BccI CCATC 3 cut(s) 271, 351, 517
BfoI RGCGCY 1 cut(s) 90
BisI GCNGC 3 cut(s) 141, 297, 307
BlpI GCTNAGC 1 cut(s) 132
BlsI GCNGC 3 cut(s) 142, 298, 308
Bme18I GGWCC 2 cut(s) 337, 536
BmeT110I CYCGRG 1 cut(s) 205
BmgT120I GGNCC 2 cut(s) 337, 536
BmsI GCATC 1 cut(s) 472
Bpu1102I GCTNAGC 1 cut(s) 132
Bsa29I ATCGAT 2 cut(s) 151, 267
BsaXI ACNNNNNCTCC 2 cut(s) 8, 38
BseCI ATCGAT 2 cut(s) 151, 267
BseMII CTCAG 2 cut(s) 132, 509
BseSI GKGCMC 1 cut(s) 385
BseXI GCAGC 2 cut(s) 127, 318
Bsh1285I CGRYCG 1 cut(s) 155
BshFI GGCC 1 cut(s) 324
BshVI ATCGAT 2 cut(s) 151, 267
BsiEI CGRYCG 1 cut(s) 155
BsiHKAI GWGCWC 2 cut(s) 352, 385
BsiHKCI CYCGRG 1 cut(s) 205
BsnI GGCC 1 cut(s) 324
BsoBI CYCGRG 1 cut(s) 205
Bsp1286I GDGCHC 2 cut(s) 352, 385
Bsp143I GATC 4 cut(s) 148, 152, 198, 268
Bsp1720I GCTNAGC 1 cut(s) 132
BspACI CCGC 2 cut(s) 56, 297
BspANI GGCC 1 cut(s) 324
BspCNI CTCAG 2 cut(s) 131, 508
BspDI ATCGAT 2 cut(s) 151, 267
BspPI GGATC 1 cut(s) 193
BsrBI CCGCTC 2 cut(s) 56, 299
BssMI GATC 4 cut(s) 148, 152, 198, 268
Bst4CI ACNGT 1 cut(s) 520
BstC8I GCNNGC 5 cut(s) 58, 62, 66, 138, 487
BstDEI CTNAG 3 cut(s) 118, 132, 495
BstH2I RGCGCY 1 cut(s) 90
BstHHI GCGC 1 cut(s) 89
BstKTI GATC 4 cut(s) 151, 155, 201, 271
BstMBI GATC 4 cut(s) 148, 152, 198, 268
BstMCI CGRYCG 1 cut(s) 155
BstMWI GCNNNNNNNGC 4 cut(s) 128, 137, 356, 433
BstSLI GKGCMC 1 cut(s) 385
BstV1I GCAGC 2 cut(s) 127, 318
BstX2I RGATCY 1 cut(s) 198
BstYI RGATCY 1 cut(s) 198
Bsu15I ATCGAT 2 cut(s) 151, 267
BsuRI GGCC 1 cut(s) 324
BsuTUI ATCGAT 2 cut(s) 151, 267
BtsIMutI CAGTG 1 cut(s) 525
Cac8I GCNNGC 5 cut(s) 58, 62, 66, 138, 487
CfoI GCGC 1 cut(s) 89
Cfr13I GGNCC 2 cut(s) 337, 536
ClaI ATCGAT 2 cut(s) 151, 267
Csp6I GTAC 1 cut(s) 569
CviAII CATG 1 cut(s) 311
CviQI GTAC 1 cut(s) 569
DdeI CTNAG 3 cut(s) 118, 132, 495
DpnI GATC 4 cut(s) 150, 154, 200, 270
DpnII GATC 4 cut(s) 148, 152, 198, 268
DraI TTTAAA 1 cut(s) 363
Eco147I AGGCCT 1 cut(s) 324
Eco47I GGWCC 2 cut(s) 337, 536
Eco88I CYCGRG 1 cut(s) 205
EcoO109I RGGNCCY 1 cut(s) 337
FaeI CATG 1 cut(s) 314
FaiI YATR 3 cut(s) 312, 330, 425
FatI CATG 1 cut(s) 310
FauI CCCGC 1 cut(s) 49
Fnu4HI GCNGC 3 cut(s) 141, 297, 307
Fsp4HI GCNGC 3 cut(s) 141, 297, 307
GlaI GCGC 1 cut(s) 88
GluI GCNGC 3 cut(s) 141, 297, 307
HaeII RGCGCY 1 cut(s) 90
HaeIII GGCC 1 cut(s) 324
HhaI GCGC 1 cut(s) 89
Hin1II CATG 1 cut(s) 314
Hin6I GCGC 1 cut(s) 87
HinP1I GCGC 1 cut(s) 87
HincII GTYRAC 1 cut(s) 159
HindII GTYRAC 1 cut(s) 159
HindIII AAGCTT 1 cut(s) 357
HinfI GANTC 3 cut(s) 126, 264, 493
HpaI GTTAAC 1 cut(s) 159
Hpy166II GTNNAC 3 cut(s) 159, 383, 569
Hpy188I TCNGA 1 cut(s) 498
Hpy188III TCNNGA 2 cut(s) 207, 261
Hpy8I GTNNAC 3 cut(s) 159, 383, 569
HpyAV CCTTC 1 cut(s) 111
HpyCH4III ACNGT 1 cut(s) 520
HpyCH4V TGCA 4 cut(s) 306, 383, 436, 503
HpyF10VI GCNNNNNNNGC 4 cut(s) 128, 137, 356, 433
HpyF3I CTNAG 3 cut(s) 118, 132, 495
Hsp92II CATG 1 cut(s) 314
HspAI GCGC 1 cut(s) 87
KspAI GTTAAC 1 cut(s) 159
Kzo9I GATC 4 cut(s) 148, 152, 198, 268
LmnI GCTCC 1 cut(s) 53
LpnPI CCDG 9 cut(s) 46, 122, 136, 246, 352, 471, 475, 549, 552
Lsp1109I GCAGC 2 cut(s) 127, 318
LweI GCATC 1 cut(s) 472
MaeIII GTNAC 2 cut(s) 92, 559
MalI GATC 4 cut(s) 150, 154, 200, 270
MbiI CCGCTC 2 cut(s) 56, 299
MboI GATC 4 cut(s) 148, 152, 198, 268
MboII GAAGA 5 cut(s) 183, 186, 189, 428, 431
MfeI CAATTG 1 cut(s) 477
MflI RGATCY 1 cut(s) 198
MhlI GDGCHC 2 cut(s) 352, 385
MluCI AATT 4 cut(s) 291, 449, 477, 508
MlyI GAGTC 2 cut(s) 120, 487
MnlI CCTC 5 cut(s) 108, 214, 229, 335, 350
MseI TTAA 2 cut(s) 158, 362
MunI CAATTG 1 cut(s) 477
MwoI GCNNNNNNNGC 4 cut(s) 128, 137, 356, 433
NdeII GATC 4 cut(s) 148, 152, 198, 268
NlaIII CATG 1 cut(s) 314
PaeR7I CTCGAG 1 cut(s) 205
PceI AGGCCT 1 cut(s) 324
PfeI GAWTC 1 cut(s) 264
PkrI GCNGC 3 cut(s) 142, 298, 308
Ple19I CGATCG 1 cut(s) 155
PleI GAGTC 2 cut(s) 120, 487
PpsI GAGTC 2 cut(s) 120, 487
PpuMI RGGWCCY 1 cut(s) 337
Psp5II RGGWCCY 1 cut(s) 337
PspPI GGNCC 2 cut(s) 337, 536
PspPPI RGGWCCY 1 cut(s) 337
PsuI RGATCY 1 cut(s) 198
PvuI CGATCG 1 cut(s) 155
RsaI GTAC 1 cut(s) 570
RsaNI GTAC 1 cut(s) 569
SaqAI TTAA 2 cut(s) 158, 362
SatI GCNGC 3 cut(s) 141, 297, 307
Sau3AI GATC 4 cut(s) 148, 152, 198, 268
Sau96I GGNCC 2 cut(s) 337, 536
SchI GAGTC 2 cut(s) 120, 487
SduI GDGCHC 2 cut(s) 352, 385
SetI ASST 7 cut(s) 221, 339, 361, 371, 541, 568, 579
SfaNI GCATC 1 cut(s) 472
Sfr274I CTCGAG 1 cut(s) 205
SinI GGWCC 2 cut(s) 337, 536
SlaI CTCGAG 1 cut(s) 205
SmlI CTYRAG 1 cut(s) 205
SmoI CTYRAG 1 cut(s) 205
Sse9I AATT 4 cut(s) 291, 449, 477, 508
SseBI AGGCCT 1 cut(s) 324
SsiI CCGC 2 cut(s) 56, 297
StuI AGGCCT 1 cut(s) 324
TaaI ACNGT 1 cut(s) 520
TaqI TCGA 4 cut(s) 151, 206, 267, 346
TasI AATT 4 cut(s) 291, 449, 477, 508
TatI WGTACW 1 cut(s) 568
TauI GCSGC 1 cut(s) 299
TfiI GAWTC 1 cut(s) 264
Tru1I TTAA 2 cut(s) 158, 362
Tru9I TTAA 2 cut(s) 158, 362
TscAI CASTG 1 cut(s) 525
TseI GCWGC 2 cut(s) 140, 306
TspRI CASTG 1 cut(s) 525
VneI GTGCAC 1 cut(s) 381
VpaK11BI GGWCC 2 cut(s) 337, 536
XapI RAATTY 1 cut(s) 508
XhoI CTCGAG 1 cut(s) 205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.