pycom17g24410

DTW

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0007999
Physical Location & Seq
Reverse (-)
22730477 .. 22734283
3807 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1161 bp
ATGGCGTCACCCGCAAGGGCTTTAACATTGAAATGCTCTCCTTGTATCGCCTTCTCCACCAAAACCCTTATGGACTCCAAACCCACAACTCTTCACAGACCCTTTTCATGTTCAATTTCTGGGTCGCCCCCACGAGAAGCTCGTACAAGCGGCGTTCAAGAAGGCGCTATCACATTGCAGGAATGGCAGGGTTGGGGCACCGCGTCTCCAGTTCCCGCCTTGGTTACCAAAATTGTTGAGGATTTGAAGGCTTTGGAAAAAGATATTGATACCCAGATGAGCTTTGGTGGTAGTCGTGGAAAACTTCAGGGAGATTTCAAAGTGCAAGAGGATAAAAAACACCGAAAAACATATCAGGCTTTAGGTGATTCTGAACAAAAGCTCCAATTCTTTTCAGCTCGACAAATAGCATGTCGTTTGCTCGGAAGTAGGGGCTACCTTTGTCAAAAGTGCTGGCTTCCCTCTGAAGAAGATTGTATGTGTTCTAGAGTCACACAATGCACTCTATGGAATAGAATGCGGTTCTGGTTATATATGCATCCCAAGGACTTCTTGCGGCAGAACAACACTGGGAAGTTGTTATGGCAAGTATTTGGCACTGAGGCTGCAACTTTGTGCCTCTTTGGCATTTCTGAACATGAAGAAATAATGTGGAATGCATTGAAGCTTGCAGGAAAAAGCAATACTTGGTGCCTGTATCCCAACAAAAATGGAGTGTTAAAATCAGTTGAGGATGTCTTTGGCCAAGAACCGTCACCATATCTGGAAGGAACAGATACTAAGACAGATAAAGATAGGACTCTGAATTTCATTTTGATTGATGGAACATGGAACAATTCAGTTGCAATATTCAGTCGGCTGAAGGATCAAGGAGTGTCAGTATGGGGAGATGGAGACTTTCCTTGTATTTCCCTGGCCACTGGTACTTCTGCGATGCACAAACTTCGGCCCCAACCATCATGGGATCGTACCTGTACAGCAGGAGCTGCTATTGGCCTCCTCTCTGAGCTGCAACTTCTTCCAGAGTTCAGCTCCTTTGGATTAGATAAACAGGCTGAAGCTGTAGAGGATACTTTAGTTTTATTATTAGAAGCACTGACAAATAGGCGGCTTCGGATGGGCAGGTCCATCACTCGCAAAGTACGACACACAATTTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DTW

Protein Analysis

387

Amino Acids

43.2

Weight (kDa)

8.69

Isoelectric Point (pI)

48.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DTW PF03942 146 - 370 7.1e-37 DTW domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1113
AccB1I GGYRCC 2 cut(s) 197, 690
AccII CGCG 1 cut(s) 203
AciI CCGC 7 cut(s) 12, 150, 201, 216, 520, 556, 1108
AclWI GGATC 2 cut(s) 873, 972
AcoI YGGCCR 2 cut(s) 742, 915
AcsI RAATTY 1 cut(s) 805
AcuI CTGAAG 4 cut(s) 290, 486, 881, 1077
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 5 cut(s) 145, 925, 970, 976, 1143
AgsI TTSAA 6 cut(s) 31, 114, 158, 247, 319, 664
AjnI CCWGG 1 cut(s) 912
AloI GAACNNNNNNTCC 2 cut(s) 366, 398
AluBI AGCT 9 cut(s) 140, 282, 382, 398, 667, 986, 1009, 1032, 1061
AluI AGCT 9 cut(s) 140, 282, 382, 398, 667, 986, 1009, 1032, 1061
Alw26I GTCTC 2 cut(s) 210, 888
AlwI GGATC 2 cut(s) 873, 972
AlwNI CAGNNNCTG 1 cut(s) 986
AoxI GGCC 4 cut(s) 742, 915, 947, 994
ApeKI GCWGC 3 cut(s) 605, 986, 1009
ApoI RAATTY 1 cut(s) 805
ArsI GACNNNNNNTTYG 3 cut(s) 397, 429, 1137
AspLEI GCGC 1 cut(s) 167
AspS9I GGNCC 2 cut(s) 948, 1125
AsuHPI GGTGA 2 cut(s) 377, 747
AvaII GGWCC 1 cut(s) 1125
BaeGI GKGCMC 1 cut(s) 200
BaeI ACNNNNGTAYC 2 cut(s) 1062, 1095
BalI TGGCCA 2 cut(s) 744, 917
BanI GGYRCC 2 cut(s) 197, 690
BauI CACGAG 1 cut(s) 132
BbvI GCAGC 3 cut(s) 592, 973, 996
BccI CCATC 5 cut(s) 815, 884, 964, 1111, 1136
BcgI CGANNNNNNTGC 2 cut(s) 926, 960
BciT130I CCWGG 1 cut(s) 914
BciVI GTATCC 2 cut(s) 708, 1063
BcoDI GTCTC 2 cut(s) 210, 888
BfaI CTAG 1 cut(s) 486
BfmI CTRYAG 1 cut(s) 1062
BfoI RGCGCY 1 cut(s) 168
BfuAI ACCTGC 1 cut(s) 1113
BfuI GTATCC 2 cut(s) 708, 1063
BglI GCCNNNNNGGC 1 cut(s) 624
BisI GCNGC 6 cut(s) 151, 557, 606, 987, 1010, 1109
BlsI GCNGC 6 cut(s) 152, 558, 607, 988, 1011, 1110
Bme1390I CCNGG 1 cut(s) 914
Bme18I GGWCC 1 cut(s) 1125
BmgT120I GGNCC 2 cut(s) 948, 1125
BmiI GGNNCC 3 cut(s) 199, 692, 950
BmrFI CCNGG 1 cut(s) 914
BmrI ACTGGG 1 cut(s) 579
BmsI GCATC 2 cut(s) 547, 924
BmuI ACTGGG 1 cut(s) 579
BplI GAGNNNNNCTC 2 cut(s) 1016, 1048
BpmI CTGGAG 1 cut(s) 192
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 3 cut(s) 219, 543, 912
BsaXI ACNNNNNCTCC 6 cut(s) 190, 220, 366, 396, 864, 894
Bse1I ACTGG 3 cut(s) 209, 574, 925
Bse3DI GCAATG 1 cut(s) 173
BseBI CCWGG 1 cut(s) 914
BseDI CCNNGG 3 cut(s) 219, 543, 912
BseGI GGATG 3 cut(s) 538, 739, 1122
BseMI GCAATG 1 cut(s) 173
BseMII CTCAG 2 cut(s) 591, 996
BseNI ACTGG 3 cut(s) 209, 574, 925
BseRI GAGGAG 1 cut(s) 989
BseSI GKGCMC 1 cut(s) 200
BseXI GCAGC 3 cut(s) 592, 973, 996
Bsh1236I CGCG 1 cut(s) 203
BshFI GGCC 4 cut(s) 744, 917, 949, 996
BshNI GGYRCC 2 cut(s) 197, 690
BsmAI GTCTC 2 cut(s) 210, 888
BsmBI CGTCTC 1 cut(s) 210
BsmI GAATGC 2 cut(s) 522, 661
BsnI GGCC 4 cut(s) 744, 917, 949, 996
Bsp1286I GDGCHC 1 cut(s) 200
Bsp1407I TGTACA 1 cut(s) 974
Bsp143I GATC 2 cut(s) 865, 964
BspACI CCGC 7 cut(s) 12, 150, 201, 216, 520, 556, 1108
BspANI GGCC 4 cut(s) 744, 917, 949, 996
BspCNI CTCAG 2 cut(s) 592, 997
BspFNI CGCG 1 cut(s) 203
BspLI GGNNCC 3 cut(s) 199, 692, 950
BspMI ACCTGC 1 cut(s) 1113
BspPI GGATC 2 cut(s) 873, 972
BspT107I GGYRCC 2 cut(s) 197, 690
BsrDI GCAATG 1 cut(s) 173
BsrGI TGTACA 1 cut(s) 974
BsrI ACTGG 3 cut(s) 209, 574, 925
BssECI CCNNGG 3 cut(s) 219, 543, 912
BssMI GATC 2 cut(s) 865, 964
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 132
BssT1I CCWWGG 2 cut(s) 219, 543
Bst2BI CACGAG 1 cut(s) 132
Bst2UI CCWGG 1 cut(s) 914
Bst4CI ACNGT 1 cut(s) 753
Bst6I CTCTTC 1 cut(s) 96
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 986
BstAUI TGTACA 1 cut(s) 974
BstC8I GCNNGC 2 cut(s) 455, 669
BstDEI CTNAG 3 cut(s) 600, 780, 1005
BstEII GGTNACC 1 cut(s) 223
BstF5I GGATG 3 cut(s) 538, 739, 1122
BstFNI CGCG 1 cut(s) 203
BstH2I RGCGCY 1 cut(s) 168
BstHHI GCGC 1 cut(s) 167
BstKTI GATC 2 cut(s) 868, 967
BstMAI GTCTC 2 cut(s) 210, 888
BstMBI GATC 2 cut(s) 865, 964
BstMWI GCNNNNNNNGC 4 cut(s) 11, 184, 624, 986
BstNI CCWGG 1 cut(s) 914
BstNSI RCATGY 1 cut(s) 414
BstPI GGTNACC 1 cut(s) 223
BstSCI CCNGG 1 cut(s) 912
BstSFI CTRYAG 1 cut(s) 1062
BstSLI GKGCMC 1 cut(s) 200
BstUI CGCG 1 cut(s) 203
BstV1I GCAGC 3 cut(s) 592, 973, 996
BsuI GTATCC 2 cut(s) 708, 1063
BsuRI GGCC 4 cut(s) 744, 917, 949, 996
BtgZI GCGATG 1 cut(s) 947
BtsCI GGATG 3 cut(s) 538, 739, 1122
BtsIMutI CAGTG 4 cut(s) 567, 597, 918, 1094
BveI ACCTGC 1 cut(s) 1113
Cac8I GCNNGC 2 cut(s) 455, 669
CaiI CAGNNNCTG 1 cut(s) 986
CfoI GCGC 1 cut(s) 167
Cfr13I GGNCC 2 cut(s) 948, 1125
CseI GACGC 1 cut(s) 192
Csp6I GTAC 5 cut(s) 144, 924, 969, 975, 1142
CviAII CATG 5 cut(s) 108, 411, 638, 828, 960
CviQI GTAC 5 cut(s) 144, 924, 969, 975, 1142
DdeI CTNAG 3 cut(s) 600, 780, 1005
DpnI GATC 2 cut(s) 867, 966
DpnII GATC 2 cut(s) 865, 964
EaeI YGGCCR 2 cut(s) 742, 915
Eam1104I CTCTTC 1 cut(s) 96
EarI CTCTTC 1 cut(s) 96
Eco130I CCWWGG 2 cut(s) 219, 543
Eco47I GGWCC 1 cut(s) 1125
Eco57I CTGAAG 4 cut(s) 290, 486, 881, 1077
Eco91I GGTNACC 1 cut(s) 223
EcoO65I GGTNACC 1 cut(s) 223
EcoRII CCWGG 1 cut(s) 912
EcoT14I CCWWGG 2 cut(s) 219, 543
EcoT22I ATGCAT 2 cut(s) 540, 661
ErhI CCWWGG 2 cut(s) 219, 543
Esp3I CGTCTC 1 cut(s) 210
FaeI CATG 5 cut(s) 111, 414, 641, 831, 963
FalI AAGNNNNNCTT 4 cut(s) 536, 568, 670, 702
FatI CATG 5 cut(s) 107, 410, 637, 827, 959
FauI CCCGC 2 cut(s) 19, 223
Fnu4HI GCNGC 6 cut(s) 151, 557, 606, 987, 1010, 1109
FokI GGATG 3 cut(s) 525, 746, 1129
Fsp4HI GCNGC 6 cut(s) 151, 557, 606, 987, 1010, 1109
FspBI CTAG 1 cut(s) 486
GlaI GCGC 1 cut(s) 166
GluI GCNGC 6 cut(s) 151, 557, 606, 987, 1010, 1109
GsuI CTGGAG 1 cut(s) 192
HaeII RGCGCY 1 cut(s) 168
HaeIII GGCC 4 cut(s) 744, 917, 949, 996
HgaI GACGC 1 cut(s) 192
HhaI GCGC 1 cut(s) 167
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 5 cut(s) 111, 414, 641, 831, 963
Hin6I GCGC 1 cut(s) 165
HinP1I GCGC 1 cut(s) 165
HindIII AAGCTT 1 cut(s) 665
HinfI GANTC 4 cut(s) 74, 368, 489, 799
HphI GGTGA 2 cut(s) 377, 747
Hpy188I TCNGA 7 cut(s) 373, 425, 466, 634, 804, 1006, 1116
Hpy188III TCNNGA 4 cut(s) 158, 486, 764, 1022
HpyAV CCTTC 5 cut(s) 61, 155, 241, 761, 856
HpyCH4III ACNGT 1 cut(s) 753
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 184, 624, 986
HpyF3I CTNAG 3 cut(s) 600, 780, 1005
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 5 cut(s) 111, 414, 641, 831, 963
HspAI GCGC 1 cut(s) 165
Kzo9I GATC 2 cut(s) 865, 964
LmnI GCTCC 3 cut(s) 387, 983, 1037
Lsp1109I GCAGC 3 cut(s) 592, 973, 996
LweI GCATC 2 cut(s) 547, 924
MaeI CTAG 1 cut(s) 486
MaeIII GTNAC 4 cut(s) 6, 223, 490, 753
MalI GATC 2 cut(s) 867, 966
MboI GATC 2 cut(s) 865, 964
MboII GAAGA 5 cut(s) 83, 479, 482, 653, 1010
MhlI GDGCHC 1 cut(s) 200
MlsI TGGCCA 2 cut(s) 744, 917
MluCI AATT 6 cut(s) 114, 231, 386, 805, 835, 1152
MluNI TGGCCA 2 cut(s) 744, 917
MlyI GAGTC 3 cut(s) 68, 498, 793
MnlI CCTC 9 cut(s) 232, 322, 472, 595, 629, 724, 1007, 1010, 1060
Mox20I TGGCCA 2 cut(s) 744, 917
Mph1103I ATGCAT 2 cut(s) 540, 661
MscI TGGCCA 2 cut(s) 744, 917
MseI TTAA 2 cut(s) 23, 719
MslI CAYNNNNRTG 1 cut(s) 31
Msp20I TGGCCA 2 cut(s) 744, 917
MspR9I CCNGG 1 cut(s) 914
Mva1269I GAATGC 2 cut(s) 522, 661
MvaI CCWGG 1 cut(s) 914
MvnI CGCG 1 cut(s) 203
MwoI GCNNNNNNNGC 4 cut(s) 11, 184, 624, 986
NdeII GATC 2 cut(s) 865, 964
NlaIII CATG 5 cut(s) 111, 414, 641, 831, 963
NlaIV GGNNCC 3 cut(s) 199, 692, 950
NmuCI GTSAC 3 cut(s) 6, 490, 753
NsiI ATGCAT 2 cut(s) 540, 661
NspI RCATGY 1 cut(s) 414
PcsI WCGNNNNNNNCGW 2 cut(s) 139, 1141
PctI GAATGC 2 cut(s) 522, 661
PfeI GAWTC 1 cut(s) 368
PkrI GCNGC 6 cut(s) 152, 558, 607, 988, 1011, 1110
PleI GAGTC 3 cut(s) 68, 497, 793
PpsI GAGTC 3 cut(s) 68, 497, 793
Psp6I CCWGG 1 cut(s) 912
PspEI GGTNACC 1 cut(s) 223
PspGI CCWGG 1 cut(s) 912
PspN4I GGNNCC 3 cut(s) 199, 692, 950
PspPI GGNCC 2 cut(s) 948, 1125
PstNI CAGNNNCTG 1 cut(s) 986
RsaI GTAC 5 cut(s) 145, 925, 970, 976, 1143
RsaNI GTAC 5 cut(s) 144, 924, 969, 975, 1142
RseI CAYNNNNRTG 1 cut(s) 31
SaqAI TTAA 2 cut(s) 23, 719
SatI GCNGC 6 cut(s) 151, 557, 606, 987, 1010, 1109
Sau3AI GATC 2 cut(s) 865, 964
Sau96I GGNCC 2 cut(s) 948, 1125
SchI GAGTC 3 cut(s) 68, 498, 793
ScrFI CCNGG 1 cut(s) 914
SduI GDGCHC 1 cut(s) 200
SfaNI GCATC 2 cut(s) 547, 924
SfcI CTRYAG 1 cut(s) 1062
SinI GGWCC 1 cut(s) 1125
SmiMI CAYNNNNRTG 1 cut(s) 31
Sse9I AATT 6 cut(s) 114, 231, 386, 805, 835, 1152
SsiI CCGC 7 cut(s) 12, 150, 201, 216, 520, 556, 1108
SspI AATATT 1 cut(s) 849
SspMI CTAG 1 cut(s) 486
StyD4I CCNGG 1 cut(s) 912
StyI CCWWGG 2 cut(s) 219, 543
TaaI ACNGT 1 cut(s) 753
TaqI TCGA 1 cut(s) 400
TasI AATT 6 cut(s) 114, 231, 386, 805, 835, 1152
TatI WGTACW 1 cut(s) 974
TauI GCSGC 3 cut(s) 153, 559, 1111
TfiI GAWTC 1 cut(s) 368
Tru1I TTAA 2 cut(s) 23, 719
Tru9I TTAA 2 cut(s) 23, 719
TscAI CASTG 4 cut(s) 574, 604, 925, 1101
TseFI GTSAC 3 cut(s) 6, 490, 753
TseI GCWGC 3 cut(s) 605, 986, 1009
Tsp45I GTSAC 3 cut(s) 6, 490, 753
TspDTI ATGAA 3 cut(s) 96, 654, 799
TspRI CASTG 4 cut(s) 574, 604, 925, 1101
VpaK11BI GGWCC 1 cut(s) 1125
XapI RAATTY 1 cut(s) 805
XbaI TCTAGA 1 cut(s) 485
XceI RCATGY 1 cut(s) 414
XcmI CCANNNNNNNNNTGG 2 cut(s) 67, 281
XspI CTAG 1 cut(s) 486
Zsp2I ATGCAT 2 cut(s) 540, 661
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.