pycom17g26220

Oxidoreductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0003907
Physical Location & Seq
Forward (+)
24570636 .. 24572614
1979 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1194 bp
ATGTCGACCAACAATAGTGGCACTTCTAAAACAAAGGCAACAACCACCACCCTCATGTCACCAGTCTCTCAACTCTCAGAGGTCAAAAATCGAAAAAAAAAAAAAATGGCAGAAAATCCAGCAGCGATACGTTTTGGCATCACTGGATGCGCCGACATAGCCAGAAAAGTAGCGCGAGCCATAAAGTTCGCACCCAACGCCACCCTCTACGCTATCGCCAGCCGTTCGATTGACAAAGCAAAGAAATTCGCAGCCGACAGTGGACTCTCCGGAGCCGTCAAGATATACGGGAGCTACGATCAACTGCTCGACGATCCATGTGTAGATGCAGTGTACATGCCCCTGCCCACAAGTCTTCACCTGCACTGGTCTGTGTTGGCTGCTCGAAAGAAGAAGCATTTGCTTTTGGAAAAACCAACGGCTCTGGATGTGGGTGAGCTTGATCGGATACTGGAAGCGTGCGAATCCAACGGTGTACAGTTCATGGATGGGAGCATGTGGCTGCATCATCCCAGGACTGCCAAGTTGAAGGACTTGGTCTCTGATCCCAAGCTTTTTGGTCAGATTGACATTATCCGTAGCACGTCAACAATGCCAGGAACCCAGGATTTCTTGGAGAGCAACATCAGAGTTAATCCGAACTTGGATGCTCTCGGTGCACTTGGGGACATAGGCTGGTACTGCATTGGGGCCATCTTGTGGGCGAAGGACTACAAATTACCAACTGTGGTGAATGCTCTACCTGATGTCCTCAAAAACTCTGCAGGGGTCATCTTGTCTTGCTCTGCATCAATTCATTGGGAACCCGCAGACAAAACCGTCGCCACAATTCACTTCTCTTTCCTCTCCCACTCGTCCATGGACTTGGCTATGACCGGTTCTAATGGTTCGATGCATTGCAACGACTACATTATTCCGTACCAAGAGAATTCGGCTTCATTTGACTTTACATCCGGCGCAACATTTGTGGAACTCCATAGCGGGTGGGATGCAAAACCAGGAGAAGTTACCGTCATTTCTGAGCTGCCGCAAGAGGCCTTGATGGTTCAAGAGTTTGCAAGGAATGCTGAGGACATTCGGAAATTTGGAAAGCGTCCGGACAGTAAATGGCCCGAGATTAGCAGAAAAACGCAGTTAGTGTTGGATGCGGTGAAGAAATCCATTGATCTTGGTTGTAAACCTGTTAAGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GFO_IDH_MocA

Protein Analysis

398

Amino Acids

43.4

Weight (kDa)

8.52

Isoelectric Point (pI)

33.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GFO_IDH_MocA PF01408 43 - 162 1.1e-17 Oxidoreductase family, NAD-binding Rossmann fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 369
AasI GACNNNNNNGTC 1 cut(s) 818
Acc36I ACCTGC 1 cut(s) 369
AccB7I CCANNNNNTGG 1 cut(s) 699
AccI GTMKAC 1 cut(s) 5
AccII CGCG 1 cut(s) 175
AccIII TCCGGA 2 cut(s) 269, 1096
AciI CCGC 4 cut(s) 807, 981, 1028, 1148
AclWI GGATC 2 cut(s) 308, 539
AcsI RAATTY 3 cut(s) 245, 928, 1082
AfaI GTAC 4 cut(s) 335, 477, 680, 920
AfiI CCNNNNNNNGG 1 cut(s) 699
AgeI ACCGGT 1 cut(s) 875
AgsI TTSAA 2 cut(s) 529, 1049
AjiI CACGTC 1 cut(s) 585
AjnI CCWGG 4 cut(s) 512, 595, 603, 997
AluBI AGCT 4 cut(s) 294, 439, 553, 1024
AluI AGCT 4 cut(s) 294, 439, 553, 1024
Alw21I GWGCWC 1 cut(s) 661
Alw26I GTCTC 2 cut(s) 70, 544
Alw44I GTGCAC 1 cut(s) 657
AlwI GGATC 2 cut(s) 308, 539
Ama87I CYCGRG 1 cut(s) 1112
Aor13HI TCCGGA 2 cut(s) 269, 1096
AoxI GGCC 3 cut(s) 690, 1035, 1109
ApaLI GTGCAC 1 cut(s) 657
ApeKI GCWGC 5 cut(s) 122, 251, 380, 502, 1024
ApoI RAATTY 3 cut(s) 245, 928, 1082
AsiGI ACCGGT 1 cut(s) 875
AspLEI GCGC 3 cut(s) 152, 175, 959
AspS9I GGNCC 2 cut(s) 690, 1110
AsuHPI GGTGA 5 cut(s) 51, 350, 446, 742, 1162
AvaI CYCGRG 1 cut(s) 1112
BaeGI GKGCMC 1 cut(s) 661
BbsI GAAGAC 1 cut(s) 347
Bbv12I GWGCWC 1 cut(s) 661
BbvCI CCTCAGC 1 cut(s) 1068
BbvI GCAGC 5 cut(s) 134, 263, 367, 489, 1011
BccI CCATC 3 cut(s) 482, 701, 1036
BceAI ACGGC 3 cut(s) 207, 260, 435
BciT130I CCWGG 4 cut(s) 514, 597, 605, 999
BciVI GTATCC 1 cut(s) 441
BcoDI GTCTC 2 cut(s) 70, 544
BfmI CTRYAG 1 cut(s) 762
BfuAI ACCTGC 1 cut(s) 369
BfuI GTATCC 1 cut(s) 441
BisI GCNGC 6 cut(s) 123, 252, 381, 503, 1025, 1028
BlsI GCNGC 6 cut(s) 124, 253, 382, 504, 1026, 1029
Bme1390I CCNGG 4 cut(s) 514, 597, 605, 999
BmeT110I CYCGRG 1 cut(s) 1112
BmgBI CACGTC 1 cut(s) 585
BmgT120I GGNCC 2 cut(s) 690, 1110
BmiI GGNNCC 4 cut(s) 274, 601, 691, 804
BmrFI CCNGG 4 cut(s) 514, 597, 605, 999
BmsI GCATC 9 cut(s) 137, 147, 316, 514, 637, 797, 882, 979, 1135
BpiI GAAGAC 1 cut(s) 347
Bpu10I CCTNAGC 1 cut(s) 1068
BsaI GGTCTC 1 cut(s) 544
BsaJI CCNNGG 3 cut(s) 512, 603, 858
BsaWI WCCGGW 3 cut(s) 269, 875, 1096
Bsc4I CCNNNNNNNGG 1 cut(s) 699
Bse118I RCCGGY 1 cut(s) 875
Bse1I ACTGG 4 cut(s) 62, 148, 371, 456
Bse3DI GCAATG 1 cut(s) 895
BseAI TCCGGA 2 cut(s) 269, 1096
BseBI CCWGG 4 cut(s) 514, 597, 605, 999
BseDI CCNNGG 3 cut(s) 512, 603, 858
BseGI GGATG 8 cut(s) 152, 433, 493, 508, 652, 950, 994, 1150
BseLI CCNNNNNNNGG 1 cut(s) 699
BseMI GCAATG 1 cut(s) 895
BseMII CTCAG 3 cut(s) 90, 1011, 1059
BseNI ACTGG 4 cut(s) 62, 148, 371, 456
BseSI GKGCMC 1 cut(s) 661
BseXI GCAGC 5 cut(s) 134, 263, 367, 489, 1011
BsgI GTGCAG 1 cut(s) 347
Bsh1236I CGCG 1 cut(s) 175
BshFI GGCC 3 cut(s) 692, 1037, 1111
BshTI ACCGGT 1 cut(s) 875
BsiHKAI GWGCWC 1 cut(s) 661
BsiHKCI CYCGRG 1 cut(s) 1112
BsiSI CCGG 4 cut(s) 270, 876, 954, 1097
BslFI GGGAC 1 cut(s) 680
BslI CCNNNNNNNGG 1 cut(s) 699
BsmAI GTCTC 2 cut(s) 70, 544
BsmFI GGGAC 1 cut(s) 680
BsmI GAATGC 2 cut(s) 739, 1069
BsnI GGCC 3 cut(s) 692, 1037, 1111
Bso31I GGTCTC 1 cut(s) 544
BsoBI CYCGRG 1 cut(s) 1112
Bsp1286I GDGCHC 1 cut(s) 661
Bsp13I TCCGGA 2 cut(s) 269, 1096
Bsp1407I TGTACA 2 cut(s) 333, 475
Bsp143I GATC 5 cut(s) 298, 313, 442, 544, 1165
Bsp19I CCATGG 1 cut(s) 858
BspACI CCGC 4 cut(s) 807, 981, 1028, 1148
BspANI GGCC 3 cut(s) 692, 1037, 1111
BspCNI CTCAG 3 cut(s) 89, 1012, 1060
BspEI TCCGGA 2 cut(s) 269, 1096
BspFNI CGCG 1 cut(s) 175
BspLI GGNNCC 4 cut(s) 274, 601, 691, 804
BspMAI CTGCAG 1 cut(s) 766
BspMI ACCTGC 1 cut(s) 369
BspPI GGATC 2 cut(s) 308, 539
BspTNI GGTCTC 1 cut(s) 544
BsrDI GCAATG 1 cut(s) 895
BsrFI RCCGGY 1 cut(s) 875
BsrGI TGTACA 2 cut(s) 333, 475
BsrI ACTGG 4 cut(s) 62, 148, 371, 456
BssAI RCCGGY 1 cut(s) 875
BssECI CCNNGG 3 cut(s) 512, 603, 858
BssMI GATC 5 cut(s) 298, 313, 442, 544, 1165
BssT1I CCWWGG 1 cut(s) 858
Bst2UI CCWGG 4 cut(s) 514, 597, 605, 999
Bst4CI ACNGT 7 cut(s) 260, 473, 480, 727, 820, 1012, 1103
BstAPI GCANNNNNTGC 1 cut(s) 1064
BstAUI TGTACA 2 cut(s) 333, 475
BstC8I GCNNGC 3 cut(s) 177, 220, 460
BstDEI CTNAG 3 cut(s) 76, 1020, 1068
BstDSI CCRYGG 1 cut(s) 858
BstF5I GGATG 8 cut(s) 152, 433, 493, 508, 652, 950, 994, 1150
BstFNI CGCG 1 cut(s) 175
BstHHI GCGC 3 cut(s) 152, 175, 959
BstKTI GATC 5 cut(s) 301, 316, 445, 547, 1168
BstMAI GTCTC 2 cut(s) 70, 544
BstMBI GATC 5 cut(s) 298, 313, 442, 544, 1165
BstMWI GCNNNNNNNGC 5 cut(s) 158, 197, 656, 681, 1064
BstNI CCWGG 4 cut(s) 514, 597, 605, 999
BstNSI RCATGY 2 cut(s) 340, 499
BstSCI CCNGG 4 cut(s) 512, 595, 603, 997
BstSFI CTRYAG 1 cut(s) 762
BstSLI GKGCMC 1 cut(s) 661
BstUI CGCG 1 cut(s) 175
BstV1I GCAGC 5 cut(s) 134, 263, 367, 489, 1011
BstV2I GAAGAC 1 cut(s) 347
BstXI CCANNNNNNTGG 1 cut(s) 865
BsuI GTATCC 1 cut(s) 441
BsuRI GGCC 3 cut(s) 692, 1037, 1111
BtgI CCRYGG 1 cut(s) 858
BtrI CACGTC 1 cut(s) 585
BtsCI GGATG 8 cut(s) 152, 433, 493, 508, 652, 950, 994, 1150
BtsI GCAGTG 1 cut(s) 336
BtsIMutI CAGTG 4 cut(s) 141, 265, 336, 364
BveI ACCTGC 1 cut(s) 369
Cac8I GCNNGC 3 cut(s) 177, 220, 460
CfoI GCGC 3 cut(s) 152, 175, 959
Cfr10I RCCGGY 1 cut(s) 875
Cfr13I GGNCC 2 cut(s) 690, 1110
CseI GACGC 1 cut(s) 1082
Csp6I GTAC 4 cut(s) 334, 476, 679, 919
CspAI ACCGGT 1 cut(s) 875
CspCI CAANNNNNGTGG 3 cut(s) 33, 948, 983
CviAII CATG 6 cut(s) 55, 318, 337, 484, 496, 859
CviQI GTAC 4 cut(s) 334, 476, 679, 919
DdeI CTNAG 3 cut(s) 76, 1020, 1068
DpnI GATC 5 cut(s) 300, 315, 444, 546, 1167
DpnII GATC 5 cut(s) 298, 313, 442, 544, 1165
DrdI GACNNNNNNGTC 1 cut(s) 818
DseDI GACNNNNNNGTC 1 cut(s) 818
Eco130I CCWWGG 1 cut(s) 858
Eco147I AGGCCT 1 cut(s) 1037
Eco31I GGTCTC 1 cut(s) 544
Eco88I CYCGRG 1 cut(s) 1112
EcoRI GAATTC 1 cut(s) 928
EcoRII CCWGG 4 cut(s) 512, 595, 603, 997
EcoT14I CCWWGG 1 cut(s) 858
EcoT22I ATGCAT 1 cut(s) 897
ErhI CCWWGG 1 cut(s) 858
FaeI CATG 6 cut(s) 58, 321, 340, 487, 499, 862
FaqI GGGAC 1 cut(s) 680
FatI CATG 6 cut(s) 54, 317, 336, 483, 495, 858
FauI CCCGC 2 cut(s) 814, 974
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 6 cut(s) 123, 252, 381, 503, 1025, 1028
FokI GGATG 8 cut(s) 159, 440, 495, 500, 659, 937, 1001, 1157
Fsp4HI GCNGC 6 cut(s) 123, 252, 381, 503, 1025, 1028
GlaI GCGC 3 cut(s) 151, 174, 958
GluI GCNGC 6 cut(s) 123, 252, 381, 503, 1025, 1028
HaeIII GGCC 3 cut(s) 692, 1037, 1111
HapII CCGG 4 cut(s) 270, 876, 954, 1097
HgaI GACGC 1 cut(s) 1082
HhaI GCGC 3 cut(s) 152, 175, 959
Hin1II CATG 6 cut(s) 58, 321, 340, 487, 499, 862
Hin6I GCGC 3 cut(s) 150, 173, 957
HinP1I GCGC 3 cut(s) 150, 173, 957
HincII GTYRAC 2 cut(s) 6, 588
HindII GTYRAC 2 cut(s) 6, 588
HindIII AAGCTT 1 cut(s) 551
HinfI GANTC 2 cut(s) 264, 464
HpaII CCGG 4 cut(s) 270, 876, 954, 1097
HphI GGTGA 5 cut(s) 51, 350, 446, 742, 1162
Hpy166II GTNNAC 7 cut(s) 6, 263, 334, 476, 588, 659, 1178
Hpy188I TCNGA 8 cut(s) 79, 447, 544, 564, 629, 639, 1021, 1080
Hpy188III TCNNGA 5 cut(s) 270, 280, 425, 1049, 1097
Hpy8I GTNNAC 7 cut(s) 6, 263, 334, 476, 588, 659, 1178
Hpy99I CGWCG 2 cut(s) 314, 824
HpyAV CCTTC 2 cut(s) 523, 700
HpyCH4III ACNGT 7 cut(s) 260, 473, 480, 727, 820, 1012, 1103
HpyCH4IV ACGT 2 cut(s) 130, 584
HpyF10VI GCNNNNNNNGC 5 cut(s) 158, 197, 656, 681, 1064
HpyF3I CTNAG 3 cut(s) 76, 1020, 1068
HpySE526I ACGT 2 cut(s) 130, 584
Hsp92II CATG 6 cut(s) 58, 321, 340, 487, 499, 862
HspAI GCGC 3 cut(s) 150, 173, 957
Kpn2I TCCGGA 2 cut(s) 269, 1096
Kzo9I GATC 5 cut(s) 298, 313, 442, 544, 1165
LmnI GCTCC 3 cut(s) 272, 291, 492
Lsp1109I GCAGC 5 cut(s) 134, 263, 367, 489, 1011
LweI GCATC 9 cut(s) 137, 147, 316, 514, 637, 797, 882, 979, 1135
MaeII ACGT 2 cut(s) 130, 584
MaeIII GTNAC 2 cut(s) 57, 1006
MalI GATC 5 cut(s) 300, 315, 444, 546, 1167
MboI GATC 5 cut(s) 298, 313, 442, 544, 1165
MboII GAAGA 3 cut(s) 347, 403, 1165
MhlI GDGCHC 1 cut(s) 661
MluCI AATT 6 cut(s) 245, 716, 792, 828, 928, 1082
MlyI GAGTC 1 cut(s) 258
MmeI TCCRAC 2 cut(s) 492, 1122
MnlI CCTC 7 cut(s) 62, 73, 215, 761, 854, 1027, 1063
Mph1103I ATGCAT 1 cut(s) 897
MroI TCCGGA 2 cut(s) 269, 1096
MseI TTAA 2 cut(s) 633, 1185
MslI CAYNNNNRTG 1 cut(s) 53
MspI CCGG 4 cut(s) 270, 876, 954, 1097
MspR9I CCNGG 4 cut(s) 514, 597, 605, 999
Mva1269I GAATGC 2 cut(s) 739, 1069
MvaI CCWGG 4 cut(s) 514, 597, 605, 999
MvnI CGCG 1 cut(s) 175
MwoI GCNNNNNNNGC 5 cut(s) 158, 197, 656, 681, 1064
NcoI CCATGG 1 cut(s) 858
NdeII GATC 5 cut(s) 298, 313, 442, 544, 1165
NlaIII CATG 6 cut(s) 58, 321, 340, 487, 499, 862
NlaIV GGNNCC 4 cut(s) 274, 601, 691, 804
NmuCI GTSAC 1 cut(s) 57
NsiI ATGCAT 1 cut(s) 897
NspI RCATGY 2 cut(s) 340, 499
PaqCI CACCTGC 1 cut(s) 369
PceI AGGCCT 1 cut(s) 1037
PcsI WCGNNNNNNNCGW 1 cut(s) 294
PctI GAATGC 2 cut(s) 739, 1069
PfeI GAWTC 1 cut(s) 464
PflFI GACNNNGTC 1 cut(s) 536
PflMI CCANNNNNTGG 1 cut(s) 699
PinAI ACCGGT 1 cut(s) 875
PkrI GCNGC 6 cut(s) 124, 253, 382, 504, 1026, 1029
PleI GAGTC 1 cut(s) 258
PpsI GAGTC 1 cut(s) 258
Psp6I CCWGG 4 cut(s) 512, 595, 603, 997
PspGI CCWGG 4 cut(s) 512, 595, 603, 997
PspN4I GGNNCC 4 cut(s) 274, 601, 691, 804
PspPI GGNCC 2 cut(s) 690, 1110
PstI CTGCAG 1 cut(s) 766
PsyI GACNNNGTC 1 cut(s) 536
RsaI GTAC 4 cut(s) 335, 477, 680, 920
RsaNI GTAC 4 cut(s) 334, 476, 679, 919
RseI CAYNNNNRTG 1 cut(s) 53
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 2 cut(s) 633, 1185
SatI GCNGC 6 cut(s) 123, 252, 381, 503, 1025, 1028
Sau3AI GATC 5 cut(s) 298, 313, 442, 544, 1165
Sau96I GGNCC 2 cut(s) 690, 1110
SchI GAGTC 1 cut(s) 258
ScrFI CCNGG 4 cut(s) 514, 597, 605, 999
SduI GDGCHC 1 cut(s) 661
SfaNI GCATC 9 cut(s) 137, 147, 316, 514, 637, 797, 882, 979, 1135
SfcI CTRYAG 1 cut(s) 762
SmiMI CAYNNNNRTG 1 cut(s) 53
Sse9I AATT 6 cut(s) 245, 716, 792, 828, 928, 1082
SseBI AGGCCT 1 cut(s) 1037
SsiI CCGC 4 cut(s) 807, 981, 1028, 1148
StuI AGGCCT 1 cut(s) 1037
StyD4I CCNGG 4 cut(s) 512, 595, 603, 997
StyI CCWWGG 1 cut(s) 858
TaaI ACNGT 7 cut(s) 260, 473, 480, 727, 820, 1012, 1103
TaiI ACGT 2 cut(s) 133, 587
TaqI TCGA 6 cut(s) 5, 91, 227, 309, 385, 890
TasI AATT 6 cut(s) 245, 716, 792, 828, 928, 1082
TatI WGTACW 2 cut(s) 333, 475
TauI GCSGC 1 cut(s) 1030
TfiI GAWTC 1 cut(s) 464
Tru1I TTAA 2 cut(s) 633, 1185
Tru9I TTAA 2 cut(s) 633, 1185
TscAI CASTG 4 cut(s) 148, 265, 336, 371
TseFI GTSAC 1 cut(s) 57
TseI GCWGC 5 cut(s) 122, 251, 380, 502, 1024
Tsp45I GTSAC 1 cut(s) 57
TspDTI ATGAA 3 cut(s) 472, 785, 927
TspGWI ACGGA 2 cut(s) 566, 906
TspRI CASTG 4 cut(s) 148, 265, 336, 371
Tth111I GACNNNGTC 1 cut(s) 536
Van91I CCANNNNNTGG 1 cut(s) 699
VneI GTGCAC 1 cut(s) 657
XapI RAATTY 3 cut(s) 245, 928, 1082
XceI RCATGY 2 cut(s) 340, 499
XmiI GTMKAC 1 cut(s) 5
Zsp2I ATGCAT 1 cut(s) 897
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.