pycom505g00120

Methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_505
OG0007018
Physical Location & Seq
Reverse (-)
258961 .. 263087
4127 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2106 bp
ATGATGACCTCCTCCTCTGTAGTTCCCGTTCAAAATGTTGATGTGCCCCAGGAGAATAAGAGTGACCTCAACGAGCAGGACAGCAATGACATTGGCGTCAAGGAACAAGTGAGTGACACCAAAAACAGGCAATTTGAAGATAACCCCGGTGACTTACCTGATGACGCAACCAAAGGAGACAGTGATGGTGCTGCTCAGGGCGAAGACAAGGTAGAAGAGAAGTCTGAAGACTCTACTGCGGAGAGATTTGTGGAAAAGACTCAAGAGACGCCTGAGGAGAAGACTGAAGACAAGATTGAGGACAAGACCGAAGACAAGATTGAGGAGAAGACTGAAGACAAGAGTGAGGAGAAAACTGAAGATGGTGACTCTAAGGCGGAAAATGGAGAATTGAATTCTGAAGATGCAGAAAAAAAAAGCGATGGTGGTGAAAACGAGAGGAAGTCTGATTCCAGTGATAATGATAAGAAGTCGGATGATGACAGTGATAAAAAATTAGACAGCTCCGAAGAAATAAATGATACAGAAAAGGTGAATGGTCAGATAGAGGAGAAACTGGAGGTAACTGATACCAAGGAGGCAGATGGGGAGAAAAAGGAAAATGAGCAGGCCAAAAACGAAAGTTCAAATGAGGTATTCCCTTCTGTTGCTCAGTCAGAGCTGTTGAACGAGACTACAACTCAAAATGGTTCGTGGTCAACTCAATCGGCAGAGTCAAAGAACGAGAAGGAGGCTCAGCGTACTTCTAATCAGAAGACCAGTTACAACTGGAAAGTCTGCAATTCCACTGCTGGGCCTGATTTCATCCCCTGCCTTGACAATTTGCAAGCCATCAAGAGTCTTCGAAGTACCAAGCATTATGAGCACCGGGAGAGGCACTGTCCCGAAGAGGCTCCCACCTGCCTTCTTCCTCTCCCAGAAGGATATAGACGCTCAATTGAGTGGCCAACAAGCAGGGAAAAGTCTGTTCCTGATATTGCCTGGGGAAAACGTTCACGTGTTGTATTAGATGTTGGATGTGGAGTTGCAAGCTTTGGAGGCTTTCTCTTTGACAGAGATGTCCTTGCAATGTCCTTGGCCCCAAAAGATGAGCATGAAGCTCAAGTTCAGTTTGCACTTGAAAGAGGAATTCCAGCCATATCTGCTGTGATGGGCACAAAGAGACTTCCCTTCCCAGGCAAAGTTTTTGATGTTGTTCACTGTGCCCGCTGCAGGGTTCCTTGGCATATAGAAGGTGGTAAGCTTCTATTGGAGCTGAATCGTGTGTTGAGACCTGGTGGTTTCTTTGTGTGGTCTGCTACTCCAATATATCAGAAGCTGGCTGAAGATGTTGAAATATGGAAATCCATGAAGGAACTAACAAAAGCAATATGCTGGGAACTGTTGTCAATTAGTAAGGATACAATAAATGGAGTTGGTATTGCCATATACAAAAAGCCTACTTCGAATGAGTGCTATGAGAAAAGATCACAGAACGAGCCTCCTATCTGCACTACGTCTGATGAGCCAAATGCTGCCTGGAATGTGCCATTACAAGCATGCATGCACAAAGTGCCTGTAGATGCAACAGAACGTGGGTCTGAATGGCCTGAACAATGGCCATCGAGGTTGGACAAGACACCTTACTGGTTGTTAAATTCCCAAGTTGGTGTTTATGGAAAACCTGCTCCTGAGGATTTCACTGCAGACAATGAGCACTGGAAACGTGTGGTGACAAAGTCATACCTGAATGGAATGGGGATTAACTGGACATCTGTCCGGAATGTTATGGACATGAGAGCTGTGTATGGAGGATTTGCTGCCGCTGTGAAAGATTTGAAAATTTGGGTCATGAATGTGGTCTCAGTAGACTCTCCAGATACACTACCCATAATTTACGAGCGTGGTTTGTTTGTAGTTGCGGAGGTTGATCGAATTCTTCGACCTGAGGGAACCCTTATCGTGCGAGACGAGGTTGAGACTATTAATGAGCTGGAGAACATGGTGAGATCGATGCAGTGGGAGGTTCGCATGACCTATTCAAAGGACAAGGAGGGATTGCTCTGTGTGAAGAAGTCCATGTGGCGGCCCAAAGAGTTGGAGACACTCAAGTATGCCATCGCTTAA

Protein Analysis

702

Amino Acids

79.03

Weight (kDa)

4.78

Isoelectric Point (pI)

45.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 267 - 321 3.6e-16 Putative S-adenosyl-L-methionine-dependent methyltransferase
Methyltransf_29 PF03141 321 - 631 3.6e-131 Putative S-adenosyl-L-methionine-dependent methyltransferase
Methyltransf_25 PF13649 335 - 427 6.6e-06 Methyltransferase domain
Methyltransf_11 PF08241 336 - 430 1.5e-06 Methyltransferase domain
Methyltransf_29 PF03141 632 - 684 7.8e-12 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 908
AasI GACNNNNNNGTC 2 cut(s) 95, 1058
Acc36I ACCTGC 2 cut(s) 908, 1672
AccI GTMKAC 1 cut(s) 1848
AccIII TCCGGA 1 cut(s) 1758
AciI CCGC 6 cut(s) 239, 377, 1207, 1803, 1901, 2065
AclI AACGTT 1 cut(s) 991
AcoI YGGCCR 2 cut(s) 944, 1598
AcsI RAATTY 5 cut(s) 394, 1128, 1636, 1821, 1914
AcuI CTGAAG 6 cut(s) 246, 306, 354, 378, 420, 1344
AcvI CACGTG 1 cut(s) 998
AcyI GRCGYC 2 cut(s) 96, 269
AdeI CACNNNGTG 1 cut(s) 1552
AfaI GTAC 2 cut(s) 742, 850
AfiI CCNNNNNNNGG 6 cut(s) 126, 792, 1175, 1212, 1213, 2064
AflIII ACRYGT 2 cut(s) 997, 1705
AgsI TTSAA 9 cut(s) 32, 137, 394, 627, 667, 1121, 1334, 1819, 2022
AjnI CCWGG 5 cut(s) 48, 980, 1174, 1273, 1517
AluBI AGCT 9 cut(s) 504, 661, 1032, 1100, 1243, 1255, 1318, 1781, 1972
AluI AGCT 9 cut(s) 504, 661, 1032, 1100, 1243, 1255, 1318, 1781, 1972
Alw21I GWGCWC 2 cut(s) 867, 1698
Alw26I GTCTC 9 cut(s) 171, 260, 665, 1156, 1264, 1846, 1941, 1952, 2075
AlwNI CAGNNNCTG 1 cut(s) 1318
Aor13HI TCCGGA 1 cut(s) 1758
AoxI GGCC 7 cut(s) 609, 794, 944, 1077, 1586, 1598, 2066
ApeKI GCWGC 4 cut(s) 191, 1209, 1514, 1799
ApoI RAATTY 5 cut(s) 394, 1128, 1636, 1821, 1914
AseI ATTAAT 1 cut(s) 1965
Asp700I GAANNNNTTC 1 cut(s) 991
AspS9I GGNCC 3 cut(s) 794, 1078, 2067
AsuC2I CCSGG 2 cut(s) 147, 869
AsuHPI GGTGA 6 cut(s) 161, 377, 440, 544, 1723, 1996
AsuII TTCGAA 2 cut(s) 844, 1445
AxyI CCTNAGG 3 cut(s) 273, 1671, 1926
BaeGI GKGCMC 3 cut(s) 48, 1157, 1207
BalI TGGCCA 2 cut(s) 946, 1600
BarI GAAGNNNNNNTAC 2 cut(s) 746, 778
BbrPI CACGTG 1 cut(s) 998
BbsI GAAGAC 9 cut(s) 210, 234, 287, 294, 318, 335, 342, 761, 833
Bbv12I GWGCWC 2 cut(s) 867, 1698
BbvI GCAGC 4 cut(s) 178, 1196, 1501, 1786
BccI CCATC 8 cut(s) 179, 356, 416, 578, 839, 1144, 1609, 2105
BciT130I CCWGG 5 cut(s) 50, 982, 1176, 1275, 1519
BciVI GTATCC 1 cut(s) 1393
BcnI CCSGG 2 cut(s) 147, 869
BcoDI GTCTC 9 cut(s) 171, 260, 665, 1156, 1264, 1846, 1941, 1952, 2075
BfmI CTRYAG 4 cut(s) 18, 1210, 1557, 1683
BfuAI ACCTGC 2 cut(s) 908, 1672
BfuI GTATCC 1 cut(s) 1393
BisI GCNGC 6 cut(s) 192, 1210, 1515, 1800, 1803, 2066
BlpI GCTNAGC 1 cut(s) 735
BlsI GCNGC 6 cut(s) 193, 1211, 1516, 1801, 1804, 2067
Bme1390I CCNGG 7 cut(s) 50, 147, 869, 982, 1176, 1275, 1519
BmgT120I GGNCC 3 cut(s) 794, 1078, 2067
BmiI GGNNCC 4 cut(s) 894, 1080, 1218, 1933
BmrFI CCNGG 7 cut(s) 50, 147, 869, 982, 1176, 1275, 1519
BmsI GCATC 3 cut(s) 394, 1552, 1983
BoxI GACNNNNGTC 1 cut(s) 1754
BpiI GAAGAC 9 cut(s) 210, 234, 287, 294, 318, 335, 342, 761, 833
BplI GAGNNNNNCTC 2 cut(s) 1029, 1061
BpmI CTGGAG 3 cut(s) 578, 1839, 1994
Bpu10I CCTNAGC 1 cut(s) 195
Bpu1102I GCTNAGC 1 cut(s) 735
Bpu14I TTCGAA 2 cut(s) 844, 1445
BpuEI CTTGAG 3 cut(s) 246, 1086, 2072
BpuMI CCSGG 2 cut(s) 147, 869
Bsa29I ATCGAT 1 cut(s) 1991
BsaAI YACGTR 1 cut(s) 998
BsaHI GRCGYC 2 cut(s) 96, 269
BsaI GGTCTC 2 cut(s) 1264, 1846
BsaJI CCNNGG 7 cut(s) 48, 145, 573, 981, 1074, 1174, 1220
BsaWI WCCGGW 1 cut(s) 1758
Bsc4I CCNNNNNNNGG 6 cut(s) 126, 792, 1175, 1212, 1213, 2064
Bse1I ACTGG 7 cut(s) 453, 561, 759, 773, 1631, 1703, 1751
Bse21I CCTNAGG 3 cut(s) 273, 1671, 1926
Bse3DI GCAATG 2 cut(s) 91, 1074
BseAI TCCGGA 1 cut(s) 1758
BseBI CCWGG 5 cut(s) 50, 982, 1176, 1275, 1519
BseCI ATCGAT 1 cut(s) 1991
BseDI CCNNGG 7 cut(s) 48, 145, 573, 981, 1074, 1174, 1220
BseGI GGATG 3 cut(s) 481, 804, 1022
BseLI CCNNNNNNNGG 6 cut(s) 126, 792, 1175, 1212, 1213, 2064
BseMI GCAATG 2 cut(s) 91, 1074
BseMII CTCAG 7 cut(s) 209, 264, 665, 749, 1662, 1857, 1917
BseNI ACTGG 7 cut(s) 453, 561, 759, 773, 1631, 1703, 1751
BseRI GAGGAG 5 cut(s) 4, 290, 338, 362, 563
BseSI GKGCMC 3 cut(s) 48, 1157, 1207
BseXI GCAGC 4 cut(s) 178, 1196, 1501, 1786
BseYI CCCAGC 2 cut(s) 791, 1374
BsgI GTGCAG 1 cut(s) 1474
BshFI GGCC 7 cut(s) 611, 796, 946, 1079, 1588, 1600, 2068
BshVI ATCGAT 1 cut(s) 1991
BsiHKAI GWGCWC 2 cut(s) 867, 1698
BsiSI CCGG 3 cut(s) 147, 868, 1759
BslFI GGGAC 1 cut(s) 867
BslI CCNNNNNNNGG 6 cut(s) 126, 792, 1175, 1212, 1213, 2064
BsmAI GTCTC 9 cut(s) 171, 260, 665, 1156, 1264, 1846, 1941, 1952, 2075
BsmBI CGTCTC 2 cut(s) 260, 1941
BsmFI GGGAC 1 cut(s) 867
BsnI GGCC 7 cut(s) 611, 796, 946, 1079, 1588, 1600, 2068
Bso31I GGTCTC 2 cut(s) 1264, 1846
Bsp119I TTCGAA 2 cut(s) 844, 1445
Bsp1286I GDGCHC 5 cut(s) 48, 867, 1157, 1207, 1698
Bsp13I TCCGGA 1 cut(s) 1758
Bsp143I GATC 3 cut(s) 1466, 1909, 1988
Bsp1720I GCTNAGC 1 cut(s) 735
BspACI CCGC 6 cut(s) 239, 377, 1207, 1803, 1901, 2065
BspANI GGCC 7 cut(s) 611, 796, 946, 1079, 1588, 1600, 2068
BspCNI CTCAG 7 cut(s) 208, 265, 664, 748, 1663, 1856, 1918
BspDI ATCGAT 1 cut(s) 1991
BspEI TCCGGA 1 cut(s) 1758
BspHI TCATGA 1 cut(s) 1830
BspLI GGNNCC 4 cut(s) 894, 1080, 1218, 1933
BspMAI CTGCAG 2 cut(s) 1214, 1687
BspMI ACCTGC 2 cut(s) 908, 1672
BspT104I TTCGAA 2 cut(s) 844, 1445
BspTNI GGTCTC 2 cut(s) 1264, 1846
BsrDI GCAATG 2 cut(s) 91, 1074
BsrI ACTGG 7 cut(s) 453, 561, 759, 773, 1631, 1703, 1751
BssECI CCNNGG 7 cut(s) 48, 145, 573, 981, 1074, 1174, 1220
BssMI GATC 3 cut(s) 1466, 1909, 1988
BssNI GRCGYC 2 cut(s) 96, 269
BssT1I CCWWGG 3 cut(s) 573, 1074, 1220
Bst2UI CCWGG 5 cut(s) 50, 982, 1176, 1275, 1519
Bst4CI ACNGT 5 cut(s) 182, 485, 881, 1202, 1383
Bst6I CTCTTC 2 cut(s) 210, 882
BstACI GRCGYC 2 cut(s) 96, 269
BstAPI GCANNNNNTGC 1 cut(s) 1552
BstBAI YACGTR 1 cut(s) 998
BstBI TTCGAA 2 cut(s) 844, 1445
BstC8I GCNNGC 7 cut(s) 609, 828, 1030, 1207, 1320, 1540, 1544
BstDEI CTNAG 8 cut(s) 195, 273, 372, 651, 735, 1671, 1843, 1926
BstF5I GGATG 3 cut(s) 481, 804, 1022
BstKTI GATC 3 cut(s) 1469, 1912, 1991
BstMAI GTCTC 9 cut(s) 171, 260, 665, 1156, 1264, 1846, 1941, 1952, 2075
BstMBI GATC 3 cut(s) 1466, 1909, 1988
BstMWI GCNNNNNNNGC 4 cut(s) 862, 1038, 1142, 1552
BstNI CCWGG 5 cut(s) 50, 982, 1176, 1275, 1519
BstNSI RCATGY 2 cut(s) 1542, 1546
BstPAI GACNNNNGTC 1 cut(s) 1754
BstSCI CCNGG 7 cut(s) 48, 145, 867, 980, 1174, 1273, 1517
BstSFI CTRYAG 4 cut(s) 18, 1210, 1557, 1683
BstSLI GKGCMC 3 cut(s) 48, 1157, 1207
BstV1I GCAGC 4 cut(s) 178, 1196, 1501, 1786
BstV2I GAAGAC 9 cut(s) 210, 234, 287, 294, 318, 335, 342, 761, 833
BstXI CCANNNNNNTGG 1 cut(s) 2077
Bsu15I ATCGAT 1 cut(s) 1991
Bsu36I CCTNAGG 3 cut(s) 273, 1671, 1926
BsuI GTATCC 1 cut(s) 1393
BsuRI GGCC 7 cut(s) 611, 796, 946, 1079, 1588, 1600, 2068
BsuTUI ATCGAT 1 cut(s) 1991
BtgZI GCGATG 2 cut(s) 435, 2083
BtsCI GGATG 3 cut(s) 481, 804, 1022
BtsI GCAGTG 3 cut(s) 786, 1680, 2003
BtsIMutI CAGTG 9 cut(s) 187, 460, 490, 786, 877, 1198, 1680, 1696, 2003
BveI ACCTGC 2 cut(s) 908, 1672
Cac8I GCNNGC 7 cut(s) 609, 828, 1030, 1207, 1320, 1540, 1544
CaiI CAGNNNCTG 1 cut(s) 1318
CciI TCATGA 1 cut(s) 1830
Cfr13I GGNCC 3 cut(s) 794, 1078, 2067
ClaI ATCGAT 1 cut(s) 1991
CseI GACGC 4 cut(s) 85, 173, 277, 939
CsiI ACCWGGT 1 cut(s) 1273
Csp6I GTAC 2 cut(s) 741, 849
CviAII CATG 9 cut(s) 1094, 1348, 1539, 1543, 1774, 1831, 1981, 2011, 2059
CviQI GTAC 2 cut(s) 741, 849
DdeI CTNAG 8 cut(s) 195, 273, 372, 651, 735, 1671, 1843, 1926
DpnI GATC 3 cut(s) 1468, 1911, 1990
DpnII GATC 3 cut(s) 1466, 1909, 1988
DraIII CACNNNGTG 1 cut(s) 1552
DrdI GACNNNNNNGTC 2 cut(s) 95, 1058
DseDI GACNNNNNNGTC 2 cut(s) 95, 1058
EaeI YGGCCR 2 cut(s) 944, 1598
Eam1104I CTCTTC 2 cut(s) 210, 882
EarI CTCTTC 2 cut(s) 210, 882
EciI GGCGGA 1 cut(s) 392
Eco130I CCWWGG 3 cut(s) 573, 1074, 1220
Eco31I GGTCTC 2 cut(s) 1264, 1846
Eco57I CTGAAG 6 cut(s) 246, 306, 354, 378, 420, 1344
Eco72I CACGTG 1 cut(s) 998
Eco81I CCTNAGG 3 cut(s) 273, 1671, 1926
EcoRI GAATTC 3 cut(s) 394, 1128, 1914
EcoRII CCWGG 5 cut(s) 48, 980, 1174, 1273, 1517
EcoT14I CCWWGG 3 cut(s) 573, 1074, 1220
EcoT22I ATGCAT 1 cut(s) 1544
ErhI CCWWGG 3 cut(s) 573, 1074, 1220
Esp3I CGTCTC 2 cut(s) 260, 1941
FaeI CATG 9 cut(s) 1097, 1351, 1542, 1546, 1777, 1834, 1984, 2014, 2062
FaqI GGGAC 1 cut(s) 867
FatI CATG 9 cut(s) 1093, 1347, 1538, 1542, 1773, 1830, 1980, 2010, 2058
FauI CCCGC 1 cut(s) 1214
FblI GTMKAC 1 cut(s) 1848
Fnu4HI GCNGC 6 cut(s) 192, 1210, 1515, 1800, 1803, 2066
FokI GGATG 3 cut(s) 488, 791, 1029
Fsp4HI GCNGC 6 cut(s) 192, 1210, 1515, 1800, 1803, 2066
GluI GCNGC 6 cut(s) 192, 1210, 1515, 1800, 1803, 2066
GsaI CCCAGC 2 cut(s) 795, 1378
GsuI CTGGAG 3 cut(s) 578, 1839, 1994
HaeIII GGCC 7 cut(s) 611, 796, 946, 1079, 1588, 1600, 2068
HapII CCGG 3 cut(s) 147, 868, 1759
HgaI GACGC 4 cut(s) 85, 173, 277, 939
Hin1I GRCGYC 2 cut(s) 96, 269
Hin1II CATG 9 cut(s) 1097, 1351, 1542, 1546, 1777, 1834, 1984, 2014, 2062
HincII GTYRAC 1 cut(s) 699
HindII GTYRAC 1 cut(s) 699
HindIII AAGCTT 2 cut(s) 1030, 1241
HinfI GANTC 8 cut(s) 230, 259, 368, 449, 713, 838, 1258, 1850
HpaII CCGG 3 cut(s) 147, 868, 1759
HphI GGTGA 6 cut(s) 161, 377, 440, 544, 1723, 1996
Hpy166II GTNNAC 4 cut(s) 699, 995, 1198, 1849
Hpy188III TCNNGA 8 cut(s) 263, 835, 884, 971, 1670, 1759, 1831, 1856
Hpy8I GTNNAC 4 cut(s) 699, 995, 1198, 1849
HpyAV CCTTC 7 cut(s) 651, 721, 914, 914, 1180, 1226, 1345
HpyCH4III ACNGT 5 cut(s) 182, 485, 881, 1202, 1383
HpyCH4IV ACGT 5 cut(s) 991, 997, 1496, 1573, 1705
HpyF10VI GCNNNNNNNGC 4 cut(s) 862, 1038, 1142, 1552
HpyF3I CTNAG 8 cut(s) 195, 273, 372, 651, 735, 1671, 1843, 1926
HpySE526I ACGT 5 cut(s) 991, 997, 1496, 1573, 1705
Hsp92I GRCGYC 2 cut(s) 96, 269
Hsp92II CATG 9 cut(s) 1097, 1351, 1542, 1546, 1777, 1834, 1984, 2014, 2062
Kpn2I TCCGGA 1 cut(s) 1758
Kzo9I GATC 3 cut(s) 1466, 1909, 1988
LmnI GCTCC 4 cut(s) 509, 898, 1252, 1672
Lsp1109I GCAGC 4 cut(s) 178, 1196, 1501, 1786
LweI GCATC 3 cut(s) 394, 1552, 1983
MabI ACCWGGT 1 cut(s) 1273
MaeII ACGT 5 cut(s) 991, 997, 1496, 1573, 1705
MaeIII GTNAC 7 cut(s) 62, 113, 149, 365, 562, 761, 1711
MalI GATC 3 cut(s) 1468, 1911, 1990
MboI GATC 3 cut(s) 1466, 1909, 1988
MfeI CAATTG 1 cut(s) 936
MhlI GDGCHC 5 cut(s) 48, 867, 1157, 1207, 1698
MlsI TGGCCA 2 cut(s) 946, 1600
MluNI TGGCCA 2 cut(s) 946, 1600
MlyI GAGTC 6 cut(s) 224, 253, 362, 722, 847, 1844
MmeI TCCRAC 4 cut(s) 453, 994, 1590, 2058
Mox20I TGGCCA 2 cut(s) 946, 1600
Mph1103I ATGCAT 1 cut(s) 1544
MroI TCCGGA 1 cut(s) 1758
MroXI GAANNNNTTC 1 cut(s) 991
MscI TGGCCA 2 cut(s) 946, 1600
MseI TTAA 4 cut(s) 1634, 1743, 1965, 2104
MslI CAYNNNNRTG 1 cut(s) 1835
Msp20I TGGCCA 2 cut(s) 946, 1600
MspA1I CMGCKG 2 cut(s) 1209, 1805
MspI CCGG 3 cut(s) 147, 868, 1759
MspR9I CCNGG 7 cut(s) 50, 147, 869, 982, 1176, 1275, 1519
MunI CAATTG 1 cut(s) 936
MvaI CCWGG 5 cut(s) 50, 982, 1176, 1275, 1519
MwoI GCNNNNNNNGC 4 cut(s) 862, 1038, 1142, 1552
NciI CCSGG 2 cut(s) 147, 869
NdeII GATC 3 cut(s) 1466, 1909, 1988
NlaIII CATG 9 cut(s) 1097, 1351, 1542, 1546, 1777, 1834, 1984, 2014, 2062
NlaIV GGNNCC 4 cut(s) 894, 1080, 1218, 1933
NmuCI GTSAC 5 cut(s) 62, 113, 149, 365, 1711
NsiI ATGCAT 1 cut(s) 1544
NspI RCATGY 2 cut(s) 1542, 1546
NspV TTCGAA 2 cut(s) 844, 1445
PaeI GCATGC 2 cut(s) 1542, 1546
PagI TCATGA 1 cut(s) 1830
PaqCI CACCTGC 1 cut(s) 908
PcsI WCGNNNNNNNCGW 2 cut(s) 1918, 1947
PdmI GAANNNNTTC 1 cut(s) 991
PfeI GAWTC 2 cut(s) 449, 1258
PflFI GACNNNGTC 1 cut(s) 1717
PkrI GCNGC 6 cut(s) 193, 1211, 1516, 1801, 1804, 2067
PleI GAGTC 6 cut(s) 224, 253, 362, 721, 846, 1844
PmaCI CACGTG 1 cut(s) 998
PmlI CACGTG 1 cut(s) 998
PpsI GAGTC 6 cut(s) 224, 253, 362, 721, 846, 1844
Ppu21I YACGTR 1 cut(s) 998
PshAI GACNNNNGTC 1 cut(s) 1754
PshBI ATTAAT 1 cut(s) 1965
Psp1406I AACGTT 1 cut(s) 991
Psp6I CCWGG 5 cut(s) 48, 980, 1174, 1273, 1517
PspCI CACGTG 1 cut(s) 998
PspFI CCCAGC 2 cut(s) 791, 1374
PspGI CCWGG 5 cut(s) 48, 980, 1174, 1273, 1517
PspN4I GGNNCC 4 cut(s) 894, 1080, 1218, 1933
PspPI GGNCC 3 cut(s) 794, 1078, 2067
PsrI GAACNNNNNNTAC 2 cut(s) 12, 44
PstI CTGCAG 2 cut(s) 1214, 1687
PstNI CAGNNNCTG 1 cut(s) 1318
PsyI GACNNNGTC 1 cut(s) 1717
RsaI GTAC 2 cut(s) 742, 850
RsaNI GTAC 2 cut(s) 741, 849
RseI CAYNNNNRTG 1 cut(s) 1835
SaqAI TTAA 4 cut(s) 1634, 1743, 1965, 2104
SatI GCNGC 6 cut(s) 192, 1210, 1515, 1800, 1803, 2066
Sau3AI GATC 3 cut(s) 1466, 1909, 1988
Sau96I GGNCC 3 cut(s) 794, 1078, 2067
SchI GAGTC 6 cut(s) 224, 253, 362, 722, 847, 1844
ScrFI CCNGG 7 cut(s) 50, 147, 869, 982, 1176, 1275, 1519
SduI GDGCHC 5 cut(s) 48, 867, 1157, 1207, 1698
SexAI ACCWGGT 1 cut(s) 1273
SfaNI GCATC 3 cut(s) 394, 1552, 1983
SfcI CTRYAG 4 cut(s) 18, 1210, 1557, 1683
SfuI TTCGAA 2 cut(s) 844, 1445
SmiMI CAYNNNNRTG 1 cut(s) 1835
SmlI CTYRAG 3 cut(s) 261, 1101, 2087
SmoI CTYRAG 3 cut(s) 261, 1101, 2087
SphI GCATGC 2 cut(s) 1542, 1546
SsiI CCGC 6 cut(s) 239, 377, 1207, 1803, 1901, 2065
StyD4I CCNGG 7 cut(s) 48, 145, 867, 980, 1174, 1273, 1517
StyI CCWWGG 3 cut(s) 573, 1074, 1220
TaaI ACNGT 5 cut(s) 182, 485, 881, 1202, 1383
TaiI ACGT 5 cut(s) 994, 1000, 1499, 1576, 1708
TaqI TCGA 6 cut(s) 844, 1445, 1604, 1912, 1921, 1991
TaqII GACCGA 1 cut(s) 323
TauI GCSGC 2 cut(s) 1805, 2068
TfiI GAWTC 2 cut(s) 449, 1258
Tru1I TTAA 4 cut(s) 1634, 1743, 1965, 2104
Tru9I TTAA 4 cut(s) 1634, 1743, 1965, 2104
TscAI CASTG 9 cut(s) 187, 460, 490, 793, 884, 1205, 1687, 1703, 2003
TseFI GTSAC 5 cut(s) 62, 113, 149, 365, 1711
TseI GCWGC 4 cut(s) 191, 1209, 1514, 1799
Tsp45I GTSAC 5 cut(s) 62, 113, 149, 365, 1711
TspDTI ATGAA 4 cut(s) 793, 1110, 1364, 1847
TspRI CASTG 9 cut(s) 187, 460, 490, 793, 884, 1205, 1687, 1703, 2003
Tth111I GACNNNGTC 1 cut(s) 1717
VspI ATTAAT 1 cut(s) 1965
XapI RAATTY 5 cut(s) 394, 1128, 1636, 1821, 1914
XceI RCATGY 2 cut(s) 1542, 1546
XcmI CCANNNNNNNNNTGG 1 cut(s) 1515
XmiI GTMKAC 1 cut(s) 1848
XmnI GAANNNNTTC 1 cut(s) 991
Zsp2I ATGCAT 1 cut(s) 1544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.