pycom5153684g00010

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012527_4311101_to_5153684
OG0004992
Physical Location & Seq
Forward (+)
20768 .. 22702
1935 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 468 bp
ATGTCTTGTTTACCAGTTGATTCAATCATGTCTTGGTACCTCGCTTTTGAGACTGGTAGGTTCTGGTTCCCCGCGCAAGTCTTTAACCGTGAGAATGGGCAAGTTGGTTTTATGTTGTCATGTTACGATGCTAAGCTTAGCTATGATCCCCGCACTGACACCTTCCAAGCCAGGTATCCGCCGCATGGAAGGAGAGCAGTTGCGATTAAGGACTGTGTGCCATGGGAAAGGCTTCGAGTGCCGCCTGTTGACAGTTCGGCTCATGATCTTCACGTCTCCGACTGTTTGAATGAGTTACGGCCTGGTGACCATATAGAGATTCAGTGGAGAAGAAACAAAGAGTTTCCTTATGGTTGGTGGTATGGTGTAGTAGGTCACCTAGAATTGTGTGATGGAAATGAAAATTACTGCCTTTGTCATAATAGTGACACGTTGGTTCAGGAGTTCAACCAATACACACATGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

156

Amino Acids

18.14

Weight (kDa)

5.56

Isoelectric Point (pI)

56.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 36
AccB1I GGYRCC 1 cut(s) 36
AccII CGCG 1 cut(s) 74
AciI CCGC 5 cut(s) 72, 151, 179, 182, 242
AclWI GGATC 1 cut(s) 140
AfaI GTAC 1 cut(s) 38
AfiI CCNNNNNNNGG 1 cut(s) 185
AflIII ACRYGT 1 cut(s) 429
AgsI TTSAA 3 cut(s) 24, 289, 448
AjiI CACGTC 1 cut(s) 274
AjnI CCWGG 2 cut(s) 170, 301
AluBI AGCT 2 cut(s) 136, 141
AluI AGCT 2 cut(s) 136, 141
Alw26I GTCTC 2 cut(s) 44, 280
AlwI GGATC 1 cut(s) 140
AoxI GGCC 1 cut(s) 299
Asp700I GAANNNNTTC 1 cut(s) 231
Asp718I GGTACC 1 cut(s) 36
AspLEI GCGC 1 cut(s) 76
AsuHPI GGTGA 2 cut(s) 317, 368
BanI GGYRCC 1 cut(s) 36
BccI CCATC 1 cut(s) 386
BceAI ACGGC 1 cut(s) 314
BciT130I CCWGG 2 cut(s) 172, 303
BciVI GTATCC 1 cut(s) 186
BcoDI GTCTC 2 cut(s) 44, 280
BfaI CTAG 1 cut(s) 380
BfuI GTATCC 1 cut(s) 186
BisI GCNGC 2 cut(s) 182, 242
BlpI GCTNAGC 2 cut(s) 132, 137
BlsI GCNGC 2 cut(s) 183, 243
Bme1390I CCNGG 2 cut(s) 172, 303
BmgBI CACGTC 1 cut(s) 274
BmiI GGNNCC 2 cut(s) 38, 68
BmrFI CCNGG 2 cut(s) 172, 303
BmsI GCATC 1 cut(s) 118
Bpu1102I GCTNAGC 2 cut(s) 132, 137
BsaJI CCNNGG 1 cut(s) 221
Bsc4I CCNNNNNNNGG 1 cut(s) 185
Bse1I ACTGG 2 cut(s) 14, 58
BseBI CCWGG 2 cut(s) 172, 303
BseDI CCNNGG 1 cut(s) 221
BseLI CCNNNNNNNGG 1 cut(s) 185
BseNI ACTGG 2 cut(s) 14, 58
Bsh1236I CGCG 1 cut(s) 74
BshFI GGCC 1 cut(s) 301
BshNI GGYRCC 1 cut(s) 36
BslI CCNNNNNNNGG 1 cut(s) 185
BsmAI GTCTC 2 cut(s) 44, 280
BsmBI CGTCTC 1 cut(s) 280
BsnI GGCC 1 cut(s) 301
Bsp143I GATC 2 cut(s) 145, 265
Bsp1720I GCTNAGC 2 cut(s) 132, 137
Bsp19I CCATGG 1 cut(s) 221
BspACI CCGC 5 cut(s) 72, 151, 179, 182, 242
BspANI GGCC 1 cut(s) 301
BspFNI CGCG 1 cut(s) 74
BspHI TCATGA 1 cut(s) 262
BspLI GGNNCC 2 cut(s) 38, 68
BspPI GGATC 1 cut(s) 140
BspT107I GGYRCC 1 cut(s) 36
BsrI ACTGG 2 cut(s) 14, 58
BssECI CCNNGG 1 cut(s) 221
BssMI GATC 2 cut(s) 145, 265
BssT1I CCWWGG 1 cut(s) 221
Bst2UI CCWGG 2 cut(s) 172, 303
Bst4CI ACNGT 4 cut(s) 89, 215, 254, 284
BstDEI CTNAG 2 cut(s) 132, 137
BstDSI CCRYGG 1 cut(s) 221
BstEII GGTNACC 2 cut(s) 305, 374
BstFNI CGCG 1 cut(s) 74
BstHHI GCGC 1 cut(s) 76
BstKTI GATC 2 cut(s) 148, 268
BstMAI GTCTC 2 cut(s) 44, 280
BstMBI GATC 2 cut(s) 145, 265
BstMWI GCNNNNNNNGC 1 cut(s) 238
BstNI CCWGG 2 cut(s) 172, 303
BstPI GGTNACC 2 cut(s) 305, 374
BstSCI CCNGG 2 cut(s) 170, 301
BstUI CGCG 1 cut(s) 74
BsuI GTATCC 1 cut(s) 186
BsuRI GGCC 1 cut(s) 301
BtgI CCRYGG 1 cut(s) 221
BtrI CACGTC 1 cut(s) 274
BtsIMutI CAGTG 2 cut(s) 153, 329
CciI TCATGA 1 cut(s) 262
CfoI GCGC 1 cut(s) 76
Csp6I GTAC 1 cut(s) 37
CviAII CATG 6 cut(s) 28, 120, 185, 222, 263, 461
CviJI RGCY 6 cut(s) 136, 141, 170, 232, 260, 301
CviKI_1 RGCY 6 cut(s) 136, 141, 170, 232, 260, 301
CviQI GTAC 1 cut(s) 37
DdeI CTNAG 2 cut(s) 132, 137
DpnI GATC 2 cut(s) 147, 267
DpnII GATC 2 cut(s) 145, 265
EciI GGCGGA 1 cut(s) 168
Eco130I CCWWGG 1 cut(s) 221
Eco91I GGTNACC 2 cut(s) 305, 374
EcoO65I GGTNACC 2 cut(s) 305, 374
EcoRII CCWGG 2 cut(s) 170, 301
EcoT14I CCWWGG 1 cut(s) 221
ErhI CCWWGG 1 cut(s) 221
Esp3I CGTCTC 1 cut(s) 280
FaeI CATG 6 cut(s) 31, 123, 188, 225, 266, 464
FatI CATG 6 cut(s) 27, 119, 184, 221, 262, 460
FauI CCCGC 2 cut(s) 79, 158
Fnu4HI GCNGC 2 cut(s) 182, 242
Fsp4HI GCNGC 2 cut(s) 182, 242
FspBI CTAG 1 cut(s) 380
GlaI GCGC 1 cut(s) 75
GluI GCNGC 2 cut(s) 182, 242
HaeIII GGCC 1 cut(s) 301
HhaI GCGC 1 cut(s) 76
Hin1II CATG 6 cut(s) 31, 123, 188, 225, 266, 464
Hin6I GCGC 1 cut(s) 74
HinP1I GCGC 1 cut(s) 74
HincII GTYRAC 1 cut(s) 250
HindII GTYRAC 1 cut(s) 250
HindIII AAGCTT 1 cut(s) 134
HinfI GANTC 2 cut(s) 20, 319
HphI GGTGA 2 cut(s) 317, 368
Hpy166II GTNNAC 2 cut(s) 11, 250
Hpy188I TCNGA 1 cut(s) 280
Hpy188III TCNNGA 2 cut(s) 263, 440
Hpy8I GTNNAC 2 cut(s) 11, 250
HpyAV CCTTC 2 cut(s) 172, 183
HpyCH4III ACNGT 4 cut(s) 89, 215, 254, 284
HpyCH4IV ACGT 2 cut(s) 273, 431
HpyF10VI GCNNNNNNNGC 1 cut(s) 238
HpyF3I CTNAG 2 cut(s) 132, 137
HpySE526I ACGT 2 cut(s) 273, 431
Hsp92II CATG 6 cut(s) 31, 123, 188, 225, 266, 464
HspAI GCGC 1 cut(s) 74
KpnI GGTACC 1 cut(s) 40
Kzo9I GATC 2 cut(s) 145, 265
LpnPI CCDG 9 cut(s) 27, 39, 49, 157, 184, 258, 288, 315, 425
LweI GCATC 1 cut(s) 118
MaeI CTAG 1 cut(s) 380
MaeII ACGT 2 cut(s) 273, 431
MaeIII GTNAC 5 cut(s) 122, 294, 305, 374, 425
MalI GATC 2 cut(s) 147, 267
MboI GATC 2 cut(s) 145, 265
MboII GAAGA 2 cut(s) 260, 342
MluCI AATT 2 cut(s) 383, 403
MmeI TCCRAC 1 cut(s) 303
MnlI CCTC 1 cut(s) 50
MroXI GAANNNNTTC 1 cut(s) 231
MseI TTAA 2 cut(s) 84, 207
MslI CAYNNNNRTG 1 cut(s) 423
MspR9I CCNGG 2 cut(s) 172, 303
MvaI CCWGG 2 cut(s) 172, 303
MvnI CGCG 1 cut(s) 74
MwoI GCNNNNNNNGC 1 cut(s) 238
NcoI CCATGG 1 cut(s) 221
NdeII GATC 2 cut(s) 145, 265
NlaIII CATG 6 cut(s) 31, 123, 188, 225, 266, 464
NlaIV GGNNCC 2 cut(s) 38, 68
NmuCI GTSAC 3 cut(s) 305, 374, 425
PagI TCATGA 1 cut(s) 262
PdmI GAANNNNTTC 1 cut(s) 231
PfeI GAWTC 2 cut(s) 20, 319
PkrI GCNGC 2 cut(s) 183, 243
Psp6I CCWGG 2 cut(s) 170, 301
PspEI GGTNACC 2 cut(s) 305, 374
PspGI CCWGG 2 cut(s) 170, 301
PspN4I GGNNCC 2 cut(s) 38, 68
RsaI GTAC 1 cut(s) 38
RsaNI GTAC 1 cut(s) 37
RseI CAYNNNNRTG 1 cut(s) 423
SaqAI TTAA 2 cut(s) 84, 207
SatI GCNGC 2 cut(s) 182, 242
Sau3AI GATC 2 cut(s) 145, 265
ScrFI CCNGG 2 cut(s) 172, 303
SfaNI GCATC 1 cut(s) 118
SmiMI CAYNNNNRTG 1 cut(s) 423
Sse9I AATT 2 cut(s) 383, 403
SsiI CCGC 5 cut(s) 72, 151, 179, 182, 242
SspMI CTAG 1 cut(s) 380
StyD4I CCNGG 2 cut(s) 170, 301
StyI CCWWGG 1 cut(s) 221
TaaI ACNGT 4 cut(s) 89, 215, 254, 284
TaiI ACGT 2 cut(s) 276, 434
TaqI TCGA 1 cut(s) 235
TasI AATT 2 cut(s) 383, 403
TauI GCSGC 2 cut(s) 184, 244
TfiI GAWTC 2 cut(s) 20, 319
Tru1I TTAA 2 cut(s) 84, 207
Tru9I TTAA 2 cut(s) 84, 207
TscAI CASTG 2 cut(s) 160, 329
TseFI GTSAC 3 cut(s) 305, 374, 425
Tsp45I GTSAC 3 cut(s) 305, 374, 425
TspDTI ATGAA 1 cut(s) 414
TspRI CASTG 2 cut(s) 160, 329
XcmI CCANNNNNNNNNTGG 1 cut(s) 458
XmnI GAANNNNTTC 1 cut(s) 231
XspI CTAG 1 cut(s) 380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.