pycom520g00580

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_520
OG0003897
Physical Location & Seq
Forward (+)
484054 .. 487244
3191 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2310 bp
ATGATTGAGAATTATTATTATTATATATATAAAGGATTGCGAAATGTTGTTTTTAAATGTCATAAGGGCATGGCCATACATAACATAGAAATCACACTTGGAAAGGGTGGACAATTAGCTAGAGCAGTAAGTGCTATGGCAAAACTGATTGCAAAAAAAGGGAAATCAGCCACACTAAAATTACCTTATAGGGAGGTCCGTTTGATATCCAAAAACTGCTCAGCAACAGTTGGACAAGTGGAGAATGTTAGGGTGAACCACAAAAGTTTGGGTAAAGCCGAATTTAAATGTTGGCTGGACCATCCTCATCGGGGTGGTGAAGGGAGGGCCCCAATTGGTAGAAAAAAACCCGCAACCCCCTGGGGTTATCCTACACTTGGAAGAAGAAGTAGAAAAAGGAATACATATAGTGATAATTTGATTCTTCGTCGCCGTATTGTGTCACATTCACTAAAAAAAAAAAAAACCTTCTGTAGCAAATCATTTATTAAGAAAAATAAATACCCTGAACGCAAAAGTTTTATTATTATCACTCACATGAGCTTCTCAGCTTATTCGGGTTTAGCTTTACTCGATGCACTATTCCCATGGTGCAGGGTTGCTTTGCATATTTTTTATGTTGAATCTCAAATTTATATTATGTATGAGTCTCAACTAATTCTATGTATTATTATACCATTACTTTCAACTGATACTAGCTATCCATCTCTCTACAAAAAACACACCTTAGAAACACCCAAACATGAAAATATATGCCCTTTTCTCTCCTTTATTGTCATTCCTTCATTCTTTCACACTAAGAAAAATAATATTATGACATTTGAATTTCATAATTCTAATTCTGAATTTCTAATACCTTCAAGTGAGCATTTTCAACCTCAATTAAAACAACCTCGAAACCTCTCATCCTTTAGGTACTTTCACTTTCTAGATTCCATTGTTTGGGCTAGATCTATCCCTCACACAACTCTCCTGGGTTTTGCCTATCGGGCAAGGTTTGTTGCTTGGTGTACTACCTTGAGGCTCACAATCAAAGCTAATATGAGTCTGAGTGTGCCACTATTGATTACTTGTACCACAAGTTACTTCGAGTTCTCAATTCGATTGGATTGTTCACAAATGGGGACTTTTACTCATAATATCATATGTCCAGTTAAGGGAGTTCAAGGTCTTTCAAGTTATTTATTTGGTTACCAGTTTAGACTAACAGTAAAAAAGAAAGAAGTTCAAAATGACATAGTAAGTACAAACAAAGGAGACTCGGGCAAGATTACAACCTTGTCTGGCAAGGTTCGTCTTCTTGCAGCCACTTTTATTTGTGTTTTACAAAGTTTGAAGTCTTGGAAAATGAAATATAAACCAGGTTTACTAAAGAGATTTGATTCTACAACACAGAGAAGGGTAGCAGAGTTGAACAAACTAAAGCTTTTTGGTTTCTTGCTTTTGGAGTGCAAAGTTGAGTGTCTTTTGATTGATTGGTTGAGAGAGTTTTTCCTTGTGCCCTTCATTGCTTTTAAAGACTTTCTATATGGTGAGTTGCCAACAACTCTAGTTATTTACCTATCCATGCCATGGAAAAGTGCTTTTTGTTGGGGTAAGCTGCCTTTTATCACTTGTCACTTCTTACATAGGCTTGTTGCCTATTCCTTGGCAAAAATGATTTTCAATTCTTCCTGGGCTGCCAACTGTCCAAGCCTAACCTCCTTCTACTTTTCGCATTCTTGGCCTTTAACCATACTAACATTGTTAAGTCTACTAATCAAGACATTGATAATGATTAAAATTAATTGCTTAAACCTTTGCACTAACCCACGATTTCCAACGTTAACTACATGCTCGTTATATAACTTCCACTTTACTCTTTTTGGCCATTACTTTCAACAAACTTTATTCTCAGGACTACCAAATTTTTCTCGAATTTTCAACCCTTCCTTGCCTCAATCTCTACGTGCCTTGCATGCGAGTCTGGATAAAGGATTGTCACTCTCTACCATCCACTCAACAACCTTCATCGTCCTCAGGCTAGCTTTAATCTACCCATTTTGTAGGAATCTCTATCTCAAAATGGTCTCACTTCAAGAGGGGTGTAGACATGTAAATTTCGTTGGACAAGTCATCAATGACATGTGGCATAAATCAAGTAAAGGAAGAATAAAACGTTCCCAAAAATTGGCAAAAGGGATAAAATATCTCTTAAAATTGGCAAAGGCCCAAAGTGCTCCTAAAACCGGAAAGAAAAACATGTGTACAGAGTGGATTTGTATTGATGAATTTGTAGGGTTACAATATATGTTTACAATTTTCCTCTGA

Protein Analysis

770

Amino Acids

88.6

Weight (kDa)

9.92

Isoelectric Point (pI)

43.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L2_C PF03947 19 - 122 2.8e-20 Ribosomal Proteins L2, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 942, 1572, 2170
AccI GTMKAC 2 cut(s) 1753, 2089
AciI CCGC 1 cut(s) 351
AclI AACGTT 2 cut(s) 1823, 2158
AcoI YGGCCR 2 cut(s) 72, 1867
AcsI RAATTY 8 cut(s) 281, 630, 824, 845, 1906, 1917, 2098, 2270
AfaI GTAC 5 cut(s) 917, 1012, 1075, 1246, 2248
AfiI CCNNNNNNNGG 7 cut(s) 311, 377, 942, 1157, 1572, 2170, 2228
AflIII ACRYGT 3 cut(s) 2092, 2124, 2241
AjnI CCWGG 4 cut(s) 359, 972, 1360, 1673
AjuI GAANNNNNNNTTGG 2 cut(s) 81, 113
AluBI AGCT 9 cut(s) 119, 543, 551, 566, 699, 1037, 1426, 1600, 2027
AluI AGCT 9 cut(s) 119, 543, 551, 566, 699, 1037, 1426, 1600, 2027
Alw21I GWGCWC 1 cut(s) 2221
Alw26I GTCTC 3 cut(s) 654, 1251, 2074
Ama87I CYCGRG 1 cut(s) 1261
AoxI GGCC 5 cut(s) 72, 327, 1724, 1867, 2208
ApaI GGGCCC 1 cut(s) 331
ApeKI GCWGC 3 cut(s) 1304, 1600, 1679
ApoI RAATTY 8 cut(s) 281, 630, 824, 845, 1906, 1917, 2098, 2270
AseI ATTAAT 1 cut(s) 1785
AspS9I GGNCC 5 cut(s) 196, 298, 327, 328, 2209
AsuHPI GGTGA 3 cut(s) 265, 329, 1544
AsuNHI GCTAGC 1 cut(s) 2023
AvaI CYCGRG 1 cut(s) 1261
AvaII GGWCC 2 cut(s) 196, 298
AxyI CCTNAGG 1 cut(s) 2018
BaeGI GKGCMC 2 cut(s) 331, 1503
BalI TGGCCA 2 cut(s) 74, 1869
BanII GRGCYC 1 cut(s) 331
BbsI GAAGAC 1 cut(s) 1289
Bbv12I GWGCWC 1 cut(s) 2221
BbvI GCAGC 3 cut(s) 1316, 1587, 1666
BccI CCATC 3 cut(s) 309, 712, 2000
BceAI ACGGC 1 cut(s) 417
BciT130I CCWGG 4 cut(s) 361, 974, 1362, 1675
BcoDI GTCTC 3 cut(s) 654, 1251, 2074
BfaI CTAG 6 cut(s) 120, 696, 929, 948, 1550, 2024
BfmI CTRYAG 1 cut(s) 472
BglI GCCNNNNNGGC 1 cut(s) 989
BglII AGATCT 1 cut(s) 950
BisI GCNGC 3 cut(s) 1305, 1601, 1680
BlpI GCTNAGC 1 cut(s) 220
BlsI GCNGC 3 cut(s) 1306, 1602, 1681
Bme1390I CCNGG 4 cut(s) 361, 974, 1362, 1675
Bme18I GGWCC 2 cut(s) 196, 298
BmeT110I CYCGRG 1 cut(s) 1261
BmgT120I GGNCC 5 cut(s) 196, 298, 327, 328, 2209
BmiI GGNNCC 2 cut(s) 329, 330
BmrFI CCNGG 4 cut(s) 361, 974, 1362, 1675
BmsI GCATC 1 cut(s) 565
BmtI GCTAGC 1 cut(s) 2027
BpiI GAAGAC 1 cut(s) 1289
Bpu1102I GCTNAGC 1 cut(s) 220
BpuEI CTTGAG 1 cut(s) 1039
BsaAI YACGTR 1 cut(s) 1949
BsaI GGTCTC 1 cut(s) 2074
BsaJI CCNNGG 7 cut(s) 359, 360, 587, 973, 1571, 1647, 1674
BsaWI WCCGGW 1 cut(s) 2228
Bsc4I CCNNNNNNNGG 7 cut(s) 311, 377, 942, 1157, 1572, 2170, 2228
Bse1I ACTGG 2 cut(s) 1151, 1195
Bse21I CCTNAGG 1 cut(s) 2018
Bse3DI GCAATG 1 cut(s) 1506
BseBI CCWGG 4 cut(s) 361, 974, 1362, 1675
BseDI CCNNGG 7 cut(s) 359, 360, 587, 973, 1571, 1647, 1674
BseGI GGATG 3 cut(s) 301, 905, 1992
BseLI CCNNNNNNNGG 7 cut(s) 311, 377, 942, 1157, 1572, 2170, 2228
BseMI GCAATG 1 cut(s) 1506
BseMII CTCAG 5 cut(s) 234, 561, 1040, 1908, 2032
BseNI ACTGG 2 cut(s) 1151, 1195
BseSI GKGCMC 2 cut(s) 331, 1503
BseXI GCAGC 3 cut(s) 1316, 1587, 1666
BsgI GTGCAG 1 cut(s) 613
BshFI GGCC 5 cut(s) 74, 329, 1726, 1869, 2210
BsiHKAI GWGCWC 1 cut(s) 2221
BsiHKCI CYCGRG 1 cut(s) 1261
BsiSI CCGG 1 cut(s) 2229
BslFI GGGAC 1 cut(s) 1138
BslI CCNNNNNNNGG 7 cut(s) 311, 377, 942, 1157, 1572, 2170, 2228
BsmAI GTCTC 3 cut(s) 654, 1251, 2074
BsmFI GGGAC 1 cut(s) 1138
BsmI GAATGC 1 cut(s) 1717
BsnI GGCC 5 cut(s) 74, 329, 1726, 1869, 2210
Bso31I GGTCTC 1 cut(s) 2074
BsoBI CYCGRG 1 cut(s) 1261
Bsp120I GGGCCC 1 cut(s) 327
Bsp1286I GDGCHC 3 cut(s) 331, 1503, 2221
Bsp1407I TGTACA 1 cut(s) 2246
Bsp143I GATC 1 cut(s) 950
Bsp1720I GCTNAGC 1 cut(s) 220
Bsp19I CCATGG 2 cut(s) 587, 1571
BspACI CCGC 1 cut(s) 351
BspANI GGCC 5 cut(s) 74, 329, 1726, 1869, 2210
BspCNI CTCAG 5 cut(s) 233, 560, 1041, 1907, 2031
BspLI GGNNCC 2 cut(s) 329, 330
BspOI GCTAGC 1 cut(s) 2027
BspTNI GGTCTC 1 cut(s) 2074
BsrDI GCAATG 1 cut(s) 1506
BsrGI TGTACA 1 cut(s) 2246
BsrI ACTGG 2 cut(s) 1151, 1195
BssECI CCNNGG 7 cut(s) 359, 360, 587, 973, 1571, 1647, 1674
BssMI GATC 1 cut(s) 950
BssT1I CCWWGG 3 cut(s) 587, 1571, 1647
Bst2UI CCWGG 4 cut(s) 361, 974, 1362, 1675
Bst4CI ACNGT 3 cut(s) 229, 1210, 1688
BstAPI GCANNNNNTGC 1 cut(s) 131
BstAUI TGTACA 1 cut(s) 2246
BstBAI YACGTR 1 cut(s) 1949
BstC8I GCNNGC 2 cut(s) 1959, 2025
BstDEI CTNAG 7 cut(s) 220, 547, 727, 798, 1049, 1894, 2018
BstDSI CCRYGG 2 cut(s) 587, 1571
BstEII GGTNACC 1 cut(s) 1190
BstF5I GGATG 3 cut(s) 301, 905, 1992
BstKTI GATC 1 cut(s) 953
BstMAI GTCTC 3 cut(s) 654, 1251, 2074
BstMBI GATC 1 cut(s) 950
BstMWI GCNNNNNNNGC 5 cut(s) 131, 989, 1723, 1958, 2216
BstNI CCWGG 4 cut(s) 361, 974, 1362, 1675
BstNSI RCATGY 5 cut(s) 1836, 1961, 2096, 2128, 2245
BstPI GGTNACC 1 cut(s) 1190
BstSCI CCNGG 4 cut(s) 359, 972, 1360, 1673
BstSFI CTRYAG 1 cut(s) 472
BstSLI GKGCMC 2 cut(s) 331, 1503
BstV1I GCAGC 3 cut(s) 1316, 1587, 1666
BstV2I GAAGAC 1 cut(s) 1289
BstX2I RGATCY 1 cut(s) 950
BstYI RGATCY 1 cut(s) 950
Bsu36I CCTNAGG 1 cut(s) 2018
BsuRI GGCC 5 cut(s) 74, 329, 1726, 1869, 2210
BtgI CCRYGG 2 cut(s) 587, 1571
BtsCI GGATG 3 cut(s) 301, 905, 1992
Cac8I GCNNGC 2 cut(s) 1959, 2025
Cfr13I GGNCC 5 cut(s) 196, 298, 327, 328, 2209
CsiI ACCWGGT 1 cut(s) 1360
Csp6I GTAC 5 cut(s) 916, 1011, 1074, 1245, 2247
CviQI GTAC 5 cut(s) 916, 1011, 1074, 1245, 2247
DdeI CTNAG 7 cut(s) 220, 547, 727, 798, 1049, 1894, 2018
DpnI GATC 1 cut(s) 952
DpnII GATC 1 cut(s) 950
DraI TTTAAA 3 cut(s) 55, 286, 1516
EaeI YGGCCR 2 cut(s) 72, 1867
Eco130I CCWWGG 3 cut(s) 587, 1571, 1647
Eco24I GRGCYC 1 cut(s) 331
Eco31I GGTCTC 1 cut(s) 2074
Eco32I GATATC 1 cut(s) 207
Eco47I GGWCC 2 cut(s) 196, 298
Eco81I CCTNAGG 1 cut(s) 2018
Eco88I CYCGRG 1 cut(s) 1261
Eco91I GGTNACC 1 cut(s) 1190
EcoO109I RGGNCCY 2 cut(s) 327, 328
EcoO65I GGTNACC 1 cut(s) 1190
EcoRII CCWGG 4 cut(s) 359, 972, 1360, 1673
EcoRV GATATC 1 cut(s) 207
EcoT14I CCWWGG 3 cut(s) 587, 1571, 1647
EcoT38I GRGCYC 1 cut(s) 331
ErhI CCWWGG 3 cut(s) 587, 1571, 1647
FaqI GGGAC 1 cut(s) 1138
FauI CCCGC 1 cut(s) 358
FauNDI CATATG 1 cut(s) 1145
FblI GTMKAC 2 cut(s) 1753, 2089
Fnu4HI GCNGC 3 cut(s) 1305, 1601, 1680
FokI GGATG 3 cut(s) 288, 892, 1979
FriOI GRGCYC 1 cut(s) 331
Fsp4HI GCNGC 3 cut(s) 1305, 1601, 1680
FspBI CTAG 6 cut(s) 120, 696, 929, 948, 1550, 2024
GluI GCNGC 3 cut(s) 1305, 1601, 1680
HaeIII GGCC 5 cut(s) 74, 329, 1726, 1869, 2210
HapII CCGG 1 cut(s) 2229
HincII GTYRAC 1 cut(s) 1827
HindII GTYRAC 1 cut(s) 1827
HindIII AAGCTT 1 cut(s) 1424
HinfI GANTC 9 cut(s) 421, 623, 647, 932, 1045, 1259, 1382, 1963, 2050
HpaI GTTAAC 1 cut(s) 1827
HpaII CCGG 1 cut(s) 2229
HphI GGTGA 3 cut(s) 265, 329, 1544
Hpy188I TCNGA 3 cut(s) 844, 1050, 2309
Hpy188III TCNNGA 6 cut(s) 929, 1762, 1896, 1914, 1967, 2078
Hpy99I CGWCG 1 cut(s) 432
HpyAV CCTTC 9 cut(s) 314, 478, 792, 867, 1392, 1513, 1714, 1938, 2017
HpyCH4III ACNGT 3 cut(s) 229, 1210, 1688
HpyCH4IV ACGT 3 cut(s) 1823, 1948, 2158
HpyCH4V TGCA 8 cut(s) 152, 578, 594, 607, 1304, 1452, 1803, 1957
HpyF10VI GCNNNNNNNGC 5 cut(s) 131, 989, 1723, 1958, 2216
HpyF3I CTNAG 7 cut(s) 220, 547, 727, 798, 1049, 1894, 2018
HpySE526I ACGT 3 cut(s) 1823, 1948, 2158
KspAI GTTAAC 1 cut(s) 1827
Kzo9I GATC 1 cut(s) 950
LmnI GCTCC 1 cut(s) 2224
Lsp1109I GCAGC 3 cut(s) 1316, 1587, 1666
LweI GCATC 1 cut(s) 565
MabI ACCWGGT 1 cut(s) 1360
MaeI CTAG 6 cut(s) 120, 696, 929, 948, 1550, 2024
MaeII ACGT 3 cut(s) 1823, 1948, 2158
MaeIII GTNAC 6 cut(s) 441, 1082, 1190, 1616, 1980, 2280
MalI GATC 1 cut(s) 952
MboI GATC 1 cut(s) 950
MboII GAAGA 6 cut(s) 393, 396, 416, 1289, 1662, 2160
MfeI CAATTG 1 cut(s) 333
MflI RGATCY 1 cut(s) 950
MhlI GDGCHC 3 cut(s) 331, 1503, 2221
MlsI TGGCCA 2 cut(s) 74, 1869
MluNI TGGCCA 2 cut(s) 74, 1869
MlyI GAGTC 4 cut(s) 656, 1054, 1253, 1972
MmeI TCCRAC 3 cut(s) 211, 1844, 2086
Mox20I TGGCCA 2 cut(s) 74, 1869
MscI TGGCCA 2 cut(s) 74, 1869
MslI CAYNNNNRTG 2 cut(s) 312, 536
Msp20I TGGCCA 2 cut(s) 74, 1869
MspI CCGG 1 cut(s) 2229
MspR9I CCNGG 4 cut(s) 361, 974, 1362, 1675
MunI CAATTG 1 cut(s) 333
Mva1269I GAATGC 1 cut(s) 1717
MvaI CCWGG 4 cut(s) 361, 974, 1362, 1675
MwoI GCNNNNNNNGC 5 cut(s) 131, 989, 1723, 1958, 2216
NcoI CCATGG 2 cut(s) 587, 1571
NdeI CATATG 1 cut(s) 1145
NdeII GATC 1 cut(s) 950
NheI GCTAGC 1 cut(s) 2023
NlaIV GGNNCC 2 cut(s) 329, 330
NmuCI GTSAC 3 cut(s) 441, 1616, 1980
NspI RCATGY 5 cut(s) 1836, 1961, 2096, 2128, 2245
PaeI GCATGC 1 cut(s) 1961
PasI CCCWGGG 1 cut(s) 360
PciI ACATGT 3 cut(s) 2092, 2124, 2241
PctI GAATGC 1 cut(s) 1717
PfeI GAWTC 5 cut(s) 421, 623, 932, 1382, 2050
PflMI CCANNNNNTGG 3 cut(s) 942, 1572, 2170
PkrI GCNGC 3 cut(s) 1306, 1602, 1681
PleI GAGTC 4 cut(s) 655, 1053, 1253, 1971
PpsI GAGTC 4 cut(s) 655, 1053, 1253, 1971
Ppu21I YACGTR 1 cut(s) 1949
PscI ACATGT 3 cut(s) 2092, 2124, 2241
PshBI ATTAAT 1 cut(s) 1785
Psp1406I AACGTT 2 cut(s) 1823, 2158
Psp6I CCWGG 4 cut(s) 359, 972, 1360, 1673
PspEI GGTNACC 1 cut(s) 1190
PspGI CCWGG 4 cut(s) 359, 972, 1360, 1673
PspN4I GGNNCC 2 cut(s) 329, 330
PspOMI GGGCCC 1 cut(s) 327
PspPI GGNCC 5 cut(s) 196, 298, 327, 328, 2209
PsuI RGATCY 1 cut(s) 950
RsaI GTAC 5 cut(s) 917, 1012, 1075, 1246, 2248
RsaNI GTAC 5 cut(s) 916, 1011, 1074, 1245, 2247
RseI CAYNNNNRTG 2 cut(s) 312, 536
SatI GCNGC 3 cut(s) 1305, 1601, 1680
Sau3AI GATC 1 cut(s) 950
Sau96I GGNCC 5 cut(s) 196, 298, 327, 328, 2209
SchI GAGTC 4 cut(s) 656, 1054, 1253, 1972
ScrFI CCNGG 4 cut(s) 361, 974, 1362, 1675
SduI GDGCHC 3 cut(s) 331, 1503, 2221
SexAI ACCWGGT 1 cut(s) 1360
SfaNI GCATC 1 cut(s) 565
SfcI CTRYAG 1 cut(s) 472
SinI GGWCC 2 cut(s) 196, 298
SmiI ATTTAAAT 1 cut(s) 286
SmiMI CAYNNNNRTG 2 cut(s) 312, 536
SmlI CTYRAG 1 cut(s) 1018
SmoI CTYRAG 1 cut(s) 1018
SphI GCATGC 1 cut(s) 1961
SsiI CCGC 1 cut(s) 351
SspI AATATT 1 cut(s) 811
SspMI CTAG 6 cut(s) 120, 696, 929, 948, 1550, 2024
StyD4I CCNGG 4 cut(s) 359, 972, 1360, 1673
StyI CCWWGG 3 cut(s) 587, 1571, 1647
SwaI ATTTAAAT 1 cut(s) 286
TaaI ACNGT 3 cut(s) 229, 1210, 1688
TaiI ACGT 3 cut(s) 1826, 1951, 2161
TaqI TCGA 5 cut(s) 573, 895, 1089, 1102, 1915
TatI WGTACW 3 cut(s) 1010, 1244, 2246
TfiI GAWTC 5 cut(s) 421, 623, 932, 1382, 2050
TseFI GTSAC 3 cut(s) 441, 1616, 1980
TseI GCWGC 3 cut(s) 1304, 1600, 1679
Tsp45I GTSAC 3 cut(s) 441, 1616, 1980
TspDTI ATGAA 7 cut(s) 759, 774, 818, 1364, 1495, 1999, 2283
TspGWI ACGGA 1 cut(s) 188
Van91I CCANNNNNTGG 3 cut(s) 942, 1572, 2170
VpaK11BI GGWCC 2 cut(s) 196, 298
VspI ATTAAT 1 cut(s) 1785
XapI RAATTY 8 cut(s) 281, 630, 824, 845, 1906, 1917, 2098, 2270
XbaI TCTAGA 1 cut(s) 928
XceI RCATGY 5 cut(s) 1836, 1961, 2096, 2128, 2245
XmiI GTMKAC 2 cut(s) 1753, 2089
XspI CTAG 6 cut(s) 120, 696, 929, 948, 1550, 2024
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.