RchiOBHm_Chr7g0192161

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0013668
Physical Location & Seq
Reverse (-)
10710929 .. 10712619
1691 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1158 bp
ATGGATCTTCTTCAATTTCTTAACATACCCTCTTTGCTTTTGGCATTCAGTTTCCTCATAGTGTTGCTTACATTCCTCTTCAAGGATGGTTCAAGAAAAAGAAAACTTAAACTCCCACCAAGCCCTCCAAAGCTACCTGTTGTTGGCAACCTTCACCAGCTAGGAAACAAGCCTCACCTATCTCTCCGGTCCTTGGCACAAAAGTATGGTCCAATAATTTACTTACAACTTGGTGAGATCCCAACTGTGGTGGTTTCATCAGCTAGACTTGCCAAGGAAGTACTGAAAACCCATGATCTTGCACTATCAAGCCGTCCACAAATCTTTTCAGCCAAACACCTCTTCTATAATTGCACTGATGTTGTCTTCTCCGCTTATGGTGCTTATTGGAGACACATACGTAAGATTTGCATACTTGAGCTGTTAAGTGCCAAAAGAGTGCAGTCATTTAGCCATGCCAGAGAGGAAGAAGTTGCTCGTCTGGCTCGTCGGGTTGCAGAGTCCTATCCCGGCACCACTAATCTCAGCAAGTTGCTTGGACTCTATGCAAATGATATTCTCTGCCGCGTAGCATTTGGAAGAGATTTCTCAGGTGGTGGAGAGTATGATCAGCATGATTTCCAAAAGATGCTTGAGGAATATCAGGAATTGCTAGGTGGATTTTCTATTGGAGATTTCTTCCCTTCCATGGAGTTCCTCCACACCTTAACAGGAATGAAATCAAGACTTCAAGACACATTCGGTCGATTTGATAAACTCTTCGACCAGATTTTGACTGAACATCTCAATCCCAAAAGAGAAAAAGAGGAGCATAAGGACCTCGTGGATGTTTTACTGGAGATACAGAAGGAAGAGACTGCCGATATGCCTCTAACCATGGATAATGTCAAGGCTATTATTTTGGACATGTTTGCTGCTGGAACAGACACAACCTTCATCACCCTTGATTGGGGGATGACAGAACTTCTGATGAACCCCAAAGTTCTGAAAAAAGCACAAGCTGAAGTAAGAGGTGTTGTTGGGGAAAAAAGAGTTGTGTTAGAGGGTGATCTTCATCAGCTGGACTATTTGAAAGCTGTCATTAAAGAGATATTTCGGCTGCATCCACCTGCACCAGTACTACCAAAACAAGAATTTTTGTCAATGCCTGGGCAATAG

Protein Analysis

385

Amino Acids

43.72

Weight (kDa)

8.25

Isoelectric Point (pI)

45.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 39 - 376 2.1e-67 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1117
Acc36I ACCTGC 1 cut(s) 1117
AccB1I GGYRCC 1 cut(s) 512
AccII CGCG 1 cut(s) 567
AciI CCGC 2 cut(s) 372, 565
AclWI GGATC 2 cut(s) 12, 232
AcsI RAATTY 1 cut(s) 1133
AcuI CTGAAG 1 cut(s) 1023
AfaI GTAC 2 cut(s) 282, 1119
AfiI CCNNNNNNNGG 7 cut(s) 82, 143, 193, 247, 688, 948, 949
AflIII ACRYGT 1 cut(s) 906
AgsI TTSAA 5 cut(s) 14, 82, 93, 731, 1072
AjnI CCWGG 1 cut(s) 1147
AjuI GAANNNNNNNTTGG 2 cut(s) 326, 358
AluBI AGCT 7 cut(s) 133, 160, 263, 421, 1001, 1060, 1076
AluI AGCT 7 cut(s) 133, 160, 263, 421, 1001, 1060, 1076
Alw26I GTCTC 2 cut(s) 385, 848
AlwI GGATC 2 cut(s) 12, 232
ApeKI GCWGC 2 cut(s) 914, 1099
ApoI RAATTY 1 cut(s) 1133
ArsI GACNNNNNNTTYG 2 cut(s) 193, 225
AspS9I GGNCC 3 cut(s) 189, 209, 817
AsuC2I CCSGG 1 cut(s) 510
AsuHPI GGTGA 5 cut(s) 146, 167, 245, 931, 1058
AvaII GGWCC 3 cut(s) 189, 209, 817
BanI GGYRCC 1 cut(s) 512
BauI CACGAG 1 cut(s) 821
BbsI GAAGAC 1 cut(s) 358
BbvI GCAGC 2 cut(s) 901, 1086
BccI CCATC 1 cut(s) 80
BceAI ACGGC 1 cut(s) 297
BciT130I CCWGG 1 cut(s) 1149
BclI TGATCA 1 cut(s) 607
BcnI CCSGG 1 cut(s) 510
BcoDI GTCTC 2 cut(s) 385, 848
BfaI CTAG 3 cut(s) 161, 264, 653
BfuAI ACCTGC 1 cut(s) 1117
BisI GCNGC 3 cut(s) 565, 915, 1100
BlsI GCNGC 3 cut(s) 566, 916, 1101
BmcAI AGTACT 2 cut(s) 282, 1119
Bme1390I CCNGG 2 cut(s) 510, 1149
Bme18I GGWCC 3 cut(s) 189, 209, 817
BmgT120I GGNCC 3 cut(s) 189, 209, 817
BmiI GGNNCC 1 cut(s) 514
BmrFI CCNGG 2 cut(s) 510, 1149
BmsI GCATC 2 cut(s) 618, 1111
BpiI GAAGAC 1 cut(s) 358
BpmI CTGGAG 1 cut(s) 857
BpuEI CTTGAG 2 cut(s) 437, 653
BpuMI CCSGG 1 cut(s) 510
BsaAI YACGTR 1 cut(s) 401
BsaBI GATNNNNATC 1 cut(s) 1053
BsaJI CCNNGG 5 cut(s) 192, 273, 687, 876, 1148
BsaWI WCCGGW 1 cut(s) 186
BsaXI ACNNNNNCTCC 2 cut(s) 96, 126
Bsc4I CCNNNNNNNGG 7 cut(s) 82, 143, 193, 247, 688, 948, 949
Bse1I ACTGG 2 cut(s) 840, 1115
Bse8I GATNNNNATC 1 cut(s) 1053
BseBI CCWGG 1 cut(s) 1149
BseDI CCNNGG 5 cut(s) 192, 273, 687, 876, 1148
BseGI GGATG 4 cut(s) 91, 832, 960, 1102
BseJI GATNNNNATC 1 cut(s) 1053
BseLI CCNNNNNNNGG 7 cut(s) 82, 143, 193, 247, 688, 948, 949
BseMII CTCAG 2 cut(s) 538, 603
BseNI ACTGG 2 cut(s) 840, 1115
BseRI GAGGAG 1 cut(s) 821
BseXI GCAGC 2 cut(s) 901, 1086
BsgI GTGCAG 2 cut(s) 461, 1095
Bsh1236I CGCG 1 cut(s) 567
Bsh1285I CGRYCG 1 cut(s) 745
BshNI GGYRCC 1 cut(s) 512
BsiEI CGRYCG 1 cut(s) 745
BsiSI CCGG 2 cut(s) 187, 510
BslI CCNNNNNNNGG 7 cut(s) 82, 143, 193, 247, 688, 948, 949
BsmAI GTCTC 2 cut(s) 385, 848
BsmI GAATGC 1 cut(s) 44
Bsp143I GATC 5 cut(s) 4, 237, 295, 607, 1048
Bsp19I CCATGG 2 cut(s) 687, 876
BspACI CCGC 2 cut(s) 372, 565
BspCNI CTCAG 2 cut(s) 537, 602
BspFNI CGCG 1 cut(s) 567
BspLI GGNNCC 1 cut(s) 514
BspMI ACCTGC 1 cut(s) 1117
BspPI GGATC 2 cut(s) 12, 232
BspT107I GGYRCC 1 cut(s) 512
BsrI ACTGG 2 cut(s) 840, 1115
BssECI CCNNGG 5 cut(s) 192, 273, 687, 876, 1148
BssMI GATC 5 cut(s) 4, 237, 295, 607, 1048
BssSI CACGAG 1 cut(s) 821
BssT1I CCWWGG 4 cut(s) 192, 273, 687, 876
Bst2BI CACGAG 1 cut(s) 821
Bst2UI CCWGG 1 cut(s) 1149
Bst4CI ACNGT 1 cut(s) 247
Bst6I CTCTTC 5 cut(s) 83, 347, 574, 764, 846
BstBAI YACGTR 1 cut(s) 401
BstDEI CTNAG 2 cut(s) 524, 589
BstDSI CCRYGG 2 cut(s) 687, 876
BstENI CCTNNNNNAGG 1 cut(s) 80
BstF5I GGATG 4 cut(s) 91, 832, 960, 1102
BstFNI CGCG 1 cut(s) 567
BstKTI GATC 5 cut(s) 7, 240, 298, 610, 1051
BstMAI GTCTC 2 cut(s) 385, 848
BstMBI GATC 5 cut(s) 4, 237, 295, 607, 1048
BstMCI CGRYCG 1 cut(s) 745
BstMWI GCNNNNNNNGC 3 cut(s) 269, 380, 482
BstNI CCWGG 1 cut(s) 1149
BstNSI RCATGY 1 cut(s) 910
BstSCI CCNGG 2 cut(s) 508, 1147
BstSNI TACGTA 1 cut(s) 401
BstUI CGCG 1 cut(s) 567
BstV1I GCAGC 2 cut(s) 901, 1086
BstV2I GAAGAC 1 cut(s) 358
BstX2I RGATCY 2 cut(s) 4, 237
BstYI RGATCY 2 cut(s) 4, 237
BtgI CCRYGG 2 cut(s) 687, 876
BtsCI GGATG 4 cut(s) 91, 832, 960, 1102
BtsIMutI CAGTG 1 cut(s) 354
BveI ACCTGC 1 cut(s) 1117
Cfr13I GGNCC 3 cut(s) 189, 209, 817
Csp6I GTAC 2 cut(s) 281, 1118
CspCI CAANNNNNGTGG 2 cut(s) 231, 266
CviAII CATG 6 cut(s) 293, 455, 614, 688, 877, 907
CviQI GTAC 2 cut(s) 281, 1118
DdeI CTNAG 2 cut(s) 524, 589
DpnI GATC 5 cut(s) 6, 239, 297, 609, 1050
DpnII GATC 5 cut(s) 4, 237, 295, 607, 1048
Eam1104I CTCTTC 5 cut(s) 83, 347, 574, 764, 846
EarI CTCTTC 5 cut(s) 83, 347, 574, 764, 846
Eco105I TACGTA 1 cut(s) 401
Eco130I CCWWGG 4 cut(s) 192, 273, 687, 876
Eco47I GGWCC 3 cut(s) 189, 209, 817
Eco57I CTGAAG 1 cut(s) 1023
EcoNI CCTNNNNNAGG 1 cut(s) 80
EcoO109I RGGNCCY 1 cut(s) 817
EcoRII CCWGG 1 cut(s) 1147
EcoT14I CCWWGG 4 cut(s) 192, 273, 687, 876
ErhI CCWWGG 4 cut(s) 192, 273, 687, 876
FaeI CATG 6 cut(s) 296, 458, 617, 691, 880, 910
FatI CATG 6 cut(s) 292, 454, 613, 687, 876, 906
FbaI TGATCA 1 cut(s) 607
Fnu4HI GCNGC 3 cut(s) 565, 915, 1100
FokI GGATG 4 cut(s) 98, 839, 967, 1089
Fsp4HI GCNGC 3 cut(s) 565, 915, 1100
FspBI CTAG 3 cut(s) 161, 264, 653
GluI GCNGC 3 cut(s) 565, 915, 1100
GsuI CTGGAG 1 cut(s) 857
HapII CCGG 2 cut(s) 187, 510
Hin1II CATG 6 cut(s) 296, 458, 617, 691, 880, 910
HinfI GANTC 2 cut(s) 500, 540
HpaII CCGG 2 cut(s) 187, 510
HphI GGTGA 5 cut(s) 146, 167, 245, 931, 1058
Hpy166II GTNNAC 1 cut(s) 317
Hpy188I TCNGA 2 cut(s) 969, 987
Hpy188III TCNNGA 4 cut(s) 93, 644, 723, 731
Hpy8I GTNNAC 1 cut(s) 317
Hpy99I CGWCG 1 cut(s) 492
HpyAV CCTTC 4 cut(s) 161, 693, 841, 943
HpyCH4III ACNGT 1 cut(s) 247
HpyCH4IV ACGT 1 cut(s) 400
HpyCH4V TGCA 8 cut(s) 302, 354, 411, 442, 497, 548, 1102, 1112
HpyF10VI GCNNNNNNNGC 3 cut(s) 269, 380, 482
HpyF3I CTNAG 2 cut(s) 524, 589
HpySE526I ACGT 1 cut(s) 400
Hsp92II CATG 6 cut(s) 296, 458, 617, 691, 880, 910
Ksp22I TGATCA 1 cut(s) 607
Kzo9I GATC 5 cut(s) 4, 237, 295, 607, 1048
LmnI GCTCC 1 cut(s) 808
Lsp1109I GCAGC 2 cut(s) 901, 1086
LweI GCATC 2 cut(s) 618, 1111
MaeI CTAG 3 cut(s) 161, 264, 653
MaeII ACGT 1 cut(s) 400
MalI GATC 5 cut(s) 6, 239, 297, 609, 1050
MboI GATC 5 cut(s) 4, 237, 295, 607, 1048
MboII GAAGA 9 cut(s) 70, 334, 358, 479, 591, 670, 751, 863, 1043
MflI RGATCY 2 cut(s) 4, 237
MluCI AATT 5 cut(s) 14, 216, 349, 647, 1133
MlyI GAGTC 2 cut(s) 509, 534
MseI TTAA 5 cut(s) 21, 108, 425, 707, 1083
MspA1I CMGCKG 1 cut(s) 1060
MspI CCGG 2 cut(s) 187, 510
MspR9I CCNGG 2 cut(s) 510, 1149
Mva1269I GAATGC 1 cut(s) 44
MvaI CCWGG 1 cut(s) 1149
MvnI CGCG 1 cut(s) 567
MwoI GCNNNNNNNGC 3 cut(s) 269, 380, 482
NciI CCSGG 1 cut(s) 510
NcoI CCATGG 2 cut(s) 687, 876
NdeII GATC 5 cut(s) 4, 237, 295, 607, 1048
NlaIII CATG 6 cut(s) 296, 458, 617, 691, 880, 910
NlaIV GGNNCC 1 cut(s) 514
NspI RCATGY 1 cut(s) 910
PaqCI CACCTGC 1 cut(s) 1117
PciI ACATGT 1 cut(s) 906
PcsI WCGNNNNNNNCGW 1 cut(s) 484
PctI GAATGC 1 cut(s) 44
PkrI GCNGC 3 cut(s) 566, 916, 1101
PleI GAGTC 2 cut(s) 508, 534
PpsI GAGTC 2 cut(s) 508, 534
Ppu21I YACGTR 1 cut(s) 401
PpuMI RGGWCCY 1 cut(s) 817
PscI ACATGT 1 cut(s) 906
Psp5II RGGWCCY 1 cut(s) 817
Psp6I CCWGG 1 cut(s) 1147
PspGI CCWGG 1 cut(s) 1147
PspN4I GGNNCC 1 cut(s) 514
PspPI GGNCC 3 cut(s) 189, 209, 817
PspPPI RGGWCCY 1 cut(s) 817
PsuI RGATCY 2 cut(s) 4, 237
PvuII CAGCTG 1 cut(s) 1060
RsaI GTAC 2 cut(s) 282, 1119
RsaNI GTAC 2 cut(s) 281, 1118
SaqAI TTAA 5 cut(s) 21, 108, 425, 707, 1083
SatI GCNGC 3 cut(s) 565, 915, 1100
Sau3AI GATC 5 cut(s) 4, 237, 295, 607, 1048
Sau96I GGNCC 3 cut(s) 189, 209, 817
ScaI AGTACT 2 cut(s) 282, 1119
SchI GAGTC 2 cut(s) 509, 534
ScrFI CCNGG 2 cut(s) 510, 1149
SfaNI GCATC 2 cut(s) 618, 1111
SinI GGWCC 3 cut(s) 189, 209, 817
SmlI CTYRAG 2 cut(s) 416, 632
SmoI CTYRAG 2 cut(s) 416, 632
SnaBI TACGTA 1 cut(s) 401
Sse9I AATT 5 cut(s) 14, 216, 349, 647, 1133
SsiI CCGC 2 cut(s) 372, 565
SspMI CTAG 3 cut(s) 161, 264, 653
StyD4I CCNGG 2 cut(s) 508, 1147
StyI CCWWGG 4 cut(s) 192, 273, 687, 876
TaaI ACNGT 1 cut(s) 247
TaiI ACGT 1 cut(s) 403
TaqI TCGA 2 cut(s) 745, 762
TaqII GACCGA 1 cut(s) 731
TasI AATT 5 cut(s) 14, 216, 349, 647, 1133
TatI WGTACW 2 cut(s) 280, 1117
TauI GCSGC 1 cut(s) 567
Tru1I TTAA 5 cut(s) 21, 108, 425, 707, 1083
Tru9I TTAA 5 cut(s) 21, 108, 425, 707, 1083
TscAI CASTG 1 cut(s) 361
TseI GCWGC 2 cut(s) 914, 1099
TspDTI ATGAA 5 cut(s) 246, 731, 925, 986, 1043
TspRI CASTG 1 cut(s) 361
VpaK11BI GGWCC 3 cut(s) 189, 209, 817
XagI CCTNNNNNAGG 1 cut(s) 80
XapI RAATTY 1 cut(s) 1133
XceI RCATGY 1 cut(s) 910
XspI CTAG 3 cut(s) 161, 264, 653
ZrmI AGTACT 2 cut(s) 282, 1119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.