RchiOBHm_Chr7g0209871

Gamma-glutamyl cyclotransferase, AIG2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0007085
Physical Location & Seq
Reverse (-)
27353803 .. 27354931
1129 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 468 bp
ATGGCTTCTCGGGTTTTCCATTCTATCCTTATTGGAGCTTTCTCTTTCTTTCTCTCTCAAGTCTCAACCAAACCATGGCTGAAACTTCCGGCGACGATTCCAAACCCCACTTCATCTTCTGCTACGTACGGTACACTCAAGCGAGGCTTCTACAACCACAAGCTCACCAAGGAATTGATCGACCAAGACGACGCCGTCTTCATCGGCCCCTACATTACCCGCCACACCTACCCCCTCGTCTGCGGCCCTCACGGCATTCAGTACCTGATCAACCTCCCCGGTTCGGGTCATCGAATCAACGGCGAGCTCTACGCACTTGTGACCCGGGGGCTCGCCCGGTTCGACGAGCTAGAAGGCACCTCGGTCGGGCTTTACGAGCGGCTCACGGTTGTTGGTGGTCAATGGGGACAAAGAGGGCGTCGTTCCGGTCGAGGCCGAGGCCTATTTCGCTCACAGAAGCTTCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GGACT

Protein Analysis

155

Amino Acids

17.28

Weight (kDa)

10.32

Isoelectric Point (pI)

29.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GGACT PF06094 42 - 130 2e-11 Gamma-glutamyl cyclotransferase, AIG2-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 194
AccB1I GGYRCC 1 cut(s) 356
AccB7I CCANNNNNTGG 1 cut(s) 75
AccBSI CCGCTC 1 cut(s) 379
AciI CCGC 3 cut(s) 220, 243, 379
AcyI GRCGYC 2 cut(s) 192, 418
AfaI GTAC 3 cut(s) 128, 133, 263
AfiI CCNNNNNNNGG 4 cut(s) 75, 283, 284, 366
AjuI GAANNNNNNNTTGG 2 cut(s) 94, 126
AluBI AGCT 5 cut(s) 38, 163, 307, 349, 460
AluI AGCT 5 cut(s) 38, 163, 307, 349, 460
Alw21I GWGCWC 1 cut(s) 309
Alw26I GTCTC 1 cut(s) 67
AlwNI CAGNNNCTG 1 cut(s) 265
Ama87I CYCGRG 2 cut(s) 9, 324
AoxI GGCC 4 cut(s) 205, 244, 433, 439
ArsI GACNNNNNNTTYG 2 cut(s) 403, 435
AspS9I GGNCC 2 cut(s) 206, 245
AsuC2I CCSGG 4 cut(s) 279, 325, 326, 337
AsuHPI GGTGA 1 cut(s) 157
AvaI CYCGRG 2 cut(s) 9, 324
BanI GGYRCC 1 cut(s) 356
BanII GRGCYC 2 cut(s) 309, 333
BbsI GAAGAC 1 cut(s) 190
Bbv12I GWGCWC 1 cut(s) 309
BceAI ACGGC 3 cut(s) 179, 268, 316
BcgI CGANNNNNNTGC 2 cut(s) 346, 380
BclI TGATCA 1 cut(s) 267
BcnI CCSGG 4 cut(s) 279, 325, 326, 337
BcoDI GTCTC 1 cut(s) 67
BfaI CTAG 1 cut(s) 350
BglI GCCNNNNNGGC 1 cut(s) 252
BisI GCNGC 2 cut(s) 244, 380
BlsI GCNGC 2 cut(s) 245, 381
Bme1390I CCNGG 4 cut(s) 279, 325, 326, 337
BmeT110I CYCGRG 2 cut(s) 9, 324
BmgT120I GGNCC 2 cut(s) 206, 245
BmiI GGNNCC 2 cut(s) 208, 358
BmrFI CCNGG 4 cut(s) 279, 325, 326, 337
BpiI GAAGAC 1 cut(s) 190
BpuEI CTTGAG 2 cut(s) 42, 122
BpuMI CCSGG 4 cut(s) 279, 325, 326, 337
BsaAI YACGTR 1 cut(s) 126
BsaHI GRCGYC 2 cut(s) 192, 418
BsaJI CCNNGG 7 cut(s) 74, 168, 277, 324, 325, 360, 436
BsaWI WCCGGW 1 cut(s) 425
Bsc4I CCNNNNNNNGG 4 cut(s) 75, 283, 284, 366
BseDI CCNNGG 7 cut(s) 74, 168, 277, 324, 325, 360, 436
BseLI CCNNNNNNNGG 4 cut(s) 75, 283, 284, 366
Bsh1285I CGRYCG 2 cut(s) 366, 430
BshFI GGCC 4 cut(s) 207, 246, 435, 441
BshNI GGYRCC 1 cut(s) 356
BsiEI CGRYCG 2 cut(s) 366, 430
BsiHKAI GWGCWC 1 cut(s) 309
BsiHKCI CYCGRG 2 cut(s) 9, 324
BsiSI CCGG 5 cut(s) 89, 279, 325, 337, 426
BsiWI CGTACG 1 cut(s) 126
BslFI GGGAC 1 cut(s) 420
BslI CCNNNNNNNGG 4 cut(s) 75, 283, 284, 366
BsmAI GTCTC 1 cut(s) 67
BsmFI GGGAC 1 cut(s) 420
BsmI GAATGC 1 cut(s) 255
BsnI GGCC 4 cut(s) 207, 246, 435, 441
BsoBI CYCGRG 2 cut(s) 9, 324
Bsp1286I GDGCHC 2 cut(s) 309, 333
Bsp143I GATC 2 cut(s) 177, 267
Bsp19I CCATGG 1 cut(s) 74
BspACI CCGC 3 cut(s) 220, 243, 379
BspANI GGCC 4 cut(s) 207, 246, 435, 441
BspLI GGNNCC 2 cut(s) 208, 358
BspT107I GGYRCC 1 cut(s) 356
BsrBI CCGCTC 1 cut(s) 379
BssECI CCNNGG 7 cut(s) 74, 168, 277, 324, 325, 360, 436
BssMI GATC 2 cut(s) 177, 267
BssNI GRCGYC 2 cut(s) 192, 418
BssT1I CCWWGG 2 cut(s) 74, 168
Bst4CI ACNGT 2 cut(s) 131, 388
BstACI GRCGYC 2 cut(s) 192, 418
BstBAI YACGTR 1 cut(s) 126
BstC8I GCNNGC 2 cut(s) 305, 333
BstDSI CCRYGG 1 cut(s) 74
BstKTI GATC 2 cut(s) 180, 270
BstMAI GTCTC 1 cut(s) 67
BstMBI GATC 2 cut(s) 177, 267
BstMCI CGRYCG 2 cut(s) 366, 430
BstMWI GCNNNNNNNGC 3 cut(s) 252, 376, 447
BstSCI CCNGG 4 cut(s) 277, 323, 324, 335
BstSNI TACGTA 1 cut(s) 126
BstV2I GAAGAC 1 cut(s) 190
BsuRI GGCC 4 cut(s) 207, 246, 435, 441
BtgI CCRYGG 1 cut(s) 74
Cac8I GCNNGC 2 cut(s) 305, 333
CaiI CAGNNNCTG 1 cut(s) 265
Cfr13I GGNCC 2 cut(s) 206, 245
Cfr9I CCCGGG 1 cut(s) 324
CseI GACGC 2 cut(s) 200, 407
Csp6I GTAC 3 cut(s) 127, 132, 262
CviAII CATG 1 cut(s) 75
CviQI GTAC 3 cut(s) 127, 132, 262
DpnI GATC 2 cut(s) 179, 269
DpnII GATC 2 cut(s) 177, 267
DrdI GACNNNNNNGTC 1 cut(s) 194
DseDI GACNNNNNNGTC 1 cut(s) 194
Ecl136II GAGCTC 1 cut(s) 307
Eco105I TACGTA 1 cut(s) 126
Eco130I CCWWGG 2 cut(s) 74, 168
Eco147I AGGCCT 1 cut(s) 441
Eco24I GRGCYC 2 cut(s) 309, 333
Eco53kI GAGCTC 1 cut(s) 307
Eco88I CYCGRG 2 cut(s) 9, 324
EcoICRI GAGCTC 1 cut(s) 307
EcoT14I CCWWGG 2 cut(s) 74, 168
EcoT38I GRGCYC 2 cut(s) 309, 333
ErhI CCWWGG 2 cut(s) 74, 168
FaeI CATG 1 cut(s) 78
FaiI YATR 1 cut(s) 76
FalI AAGNNNNNCTT 2 cut(s) 131, 163
FaqI GGGAC 1 cut(s) 420
FatI CATG 1 cut(s) 74
FauI CCCGC 1 cut(s) 227
FbaI TGATCA 1 cut(s) 267
Fnu4HI GCNGC 2 cut(s) 244, 380
FriOI GRGCYC 2 cut(s) 309, 333
Fsp4HI GCNGC 2 cut(s) 244, 380
FspBI CTAG 1 cut(s) 350
GluI GCNGC 2 cut(s) 244, 380
HaeIII GGCC 4 cut(s) 207, 246, 435, 441
HapII CCGG 5 cut(s) 89, 279, 325, 337, 426
HgaI GACGC 2 cut(s) 200, 407
Hin1I GRCGYC 2 cut(s) 192, 418
Hin1II CATG 1 cut(s) 78
HindIII AAGCTT 1 cut(s) 458
HinfI GANTC 2 cut(s) 97, 294
HpaII CCGG 5 cut(s) 89, 279, 325, 337, 426
HphI GGTGA 1 cut(s) 157
Hpy166II GTNNAC 1 cut(s) 134
Hpy8I GTNNAC 1 cut(s) 134
Hpy99I CGWCG 4 cut(s) 97, 194, 347, 423
HpyAV CCTTC 1 cut(s) 347
HpyCH4III ACNGT 2 cut(s) 131, 388
HpyCH4IV ACGT 1 cut(s) 125
HpyF10VI GCNNNNNNNGC 3 cut(s) 252, 376, 447
HpySE526I ACGT 1 cut(s) 125
Hsp92I GRCGYC 2 cut(s) 192, 418
Hsp92II CATG 1 cut(s) 78
Ksp22I TGATCA 1 cut(s) 267
Kzo9I GATC 2 cut(s) 177, 267
LmnI GCTCC 1 cut(s) 35
LpnPI CCDG 6 cut(s) 102, 278, 292, 338, 350, 439
MaeI CTAG 1 cut(s) 350
MaeII ACGT 1 cut(s) 125
MaeIII GTNAC 1 cut(s) 319
MalI GATC 2 cut(s) 179, 269
MbiI CCGCTC 1 cut(s) 379
MboI GATC 2 cut(s) 177, 267
MboII GAAGA 2 cut(s) 108, 190
MhlI GDGCHC 2 cut(s) 309, 333
MluCI AATT 1 cut(s) 173
MnlI CCTC 8 cut(s) 137, 245, 258, 284, 370, 407, 425, 431
MspI CCGG 5 cut(s) 89, 279, 325, 337, 426
MspR9I CCNGG 4 cut(s) 279, 325, 326, 337
Mva1269I GAATGC 1 cut(s) 255
MwoI GCNNNNNNNGC 3 cut(s) 252, 376, 447
NciI CCSGG 4 cut(s) 279, 325, 326, 337
NcoI CCATGG 1 cut(s) 74
NdeII GATC 2 cut(s) 177, 267
NlaIII CATG 1 cut(s) 78
NlaIV GGNNCC 2 cut(s) 208, 358
NmeAIII GCCGAG 1 cut(s) 461
NmuCI GTSAC 1 cut(s) 319
PceI AGGCCT 1 cut(s) 441
PcsI WCGNNNNNNNCGW 4 cut(s) 186, 339, 372, 427
PctI GAATGC 1 cut(s) 255
PfeI GAWTC 2 cut(s) 97, 294
Pfl23II CGTACG 1 cut(s) 126
PflFI GACNNNGTC 1 cut(s) 194
PflMI CCANNNNNTGG 1 cut(s) 75
PkrI GCNGC 2 cut(s) 245, 381
Ppu21I YACGTR 1 cut(s) 126
Psp124BI GAGCTC 1 cut(s) 309
PspLI CGTACG 1 cut(s) 126
PspN4I GGNNCC 2 cut(s) 208, 358
PspPI GGNCC 2 cut(s) 206, 245
PstNI CAGNNNCTG 1 cut(s) 265
PsyI GACNNNGTC 1 cut(s) 194
RsaI GTAC 3 cut(s) 128, 133, 263
RsaNI GTAC 3 cut(s) 127, 132, 262
SacI GAGCTC 1 cut(s) 309
SatI GCNGC 2 cut(s) 244, 380
Sau3AI GATC 2 cut(s) 177, 267
Sau96I GGNCC 2 cut(s) 206, 245
ScrFI CCNGG 4 cut(s) 279, 325, 326, 337
SduI GDGCHC 2 cut(s) 309, 333
SmaI CCCGGG 1 cut(s) 326
SmlI CTYRAG 2 cut(s) 57, 137
SmoI CTYRAG 2 cut(s) 57, 137
SnaBI TACGTA 1 cut(s) 126
Sse9I AATT 1 cut(s) 173
SseBI AGGCCT 1 cut(s) 441
SsiI CCGC 3 cut(s) 220, 243, 379
SspMI CTAG 1 cut(s) 350
SstI GAGCTC 1 cut(s) 309
StuI AGGCCT 1 cut(s) 441
StyD4I CCNGG 4 cut(s) 277, 323, 324, 335
StyI CCWWGG 2 cut(s) 74, 168
TaaI ACNGT 2 cut(s) 131, 388
TaiI ACGT 1 cut(s) 128
TaqI TCGA 4 cut(s) 180, 292, 342, 430
TaqII GACCGA 1 cut(s) 352
TasI AATT 1 cut(s) 173
TauI GCSGC 2 cut(s) 246, 382
TfiI GAWTC 2 cut(s) 97, 294
TseFI GTSAC 1 cut(s) 319
Tsp45I GTSAC 1 cut(s) 319
TspDTI ATGAA 2 cut(s) 102, 190
TspMI CCCGGG 1 cut(s) 324
Tth111I GACNNNGTC 1 cut(s) 194
Van91I CCANNNNNTGG 1 cut(s) 75
XmaI CCCGGG 1 cut(s) 324
XspI CTAG 1 cut(s) 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.