RchiOBHm_Chr6g0248511

Thymocyte nuclear protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0012175
Physical Location & Seq
Forward (+)
4530360 .. 4531115
756 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 477 bp
ATGGGCAAAGAGAAGCAGTATTGGCTTCTGAAAACAGAGCCAGGAGAGTGGTCGTGGGAGGACCAAGCAGCCAATGAGGGCATCACCAAGTGGGATGGAGTCAAGAACAAGCAGGCCCAGAAGTACCTCAAGTCCATGAACCTGGACGACCTCTGCTTCTTCTACCACTCCGGCGCCAAGGCCCGCCGCATAGTCGGCGTCGTGACGGTCGTGCGTGAATGGTATTCGGAAGGTGAAGGTGGAGATGGTGCGGTTGATGTGAAGGCAGTTGGGGAGATGAGGAGGCCGGTTGACTTGAAGGAGATGAAGGGGGAGGAGGGTCTCAAGGGTTTTGCTCTGTTTCGGCAGCCGAGGCTGTCGGTTGTGCCGGTTTCGGAGGATGTGTGGTTGAAAGTTTGTGATTTGGGAGGTGGGTTTGAAGGTGATGGTGTTGTTGTGGAAGATGATGAATCTGGTGATGAAGGAGACGATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EVE

Protein Analysis

158

Amino Acids

17.62

Weight (kDa)

4.6

Isoelectric Point (pI)

32.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EVE PF01878 6 - 134 2.7e-37 EVE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 173
AciI CCGC 3 cut(s) 184, 187, 251
AcyI GRCGYC 2 cut(s) 174, 198
AdeI CACNNNGTG 1 cut(s) 90
AfaI GTAC 1 cut(s) 125
AgsI TTSAA 3 cut(s) 298, 391, 419
AjnI CCWGG 2 cut(s) 40, 141
Alw26I GTCTC 2 cut(s) 326, 459
AoxI GGCC 3 cut(s) 114, 180, 284
ApeKI GCWGC 2 cut(s) 68, 346
AspLEI GCGC 1 cut(s) 176
AspS9I GGNCC 3 cut(s) 61, 115, 181
AsuHPI GGTGA 4 cut(s) 76, 245, 434, 467
AvaII GGWCC 1 cut(s) 61
BanI GGYRCC 1 cut(s) 173
BbvI GCAGC 2 cut(s) 80, 358
BccI CCATC 3 cut(s) 89, 239, 419
BciT130I CCWGG 2 cut(s) 42, 143
BcoDI GTCTC 2 cut(s) 326, 459
BfoI RGCGCY 1 cut(s) 177
BisI GCNGC 3 cut(s) 69, 187, 347
BlsI GCNGC 3 cut(s) 70, 188, 348
Bme1390I CCNGG 2 cut(s) 42, 143
Bme18I GGWCC 1 cut(s) 61
BmgT120I GGNCC 3 cut(s) 61, 115, 181
BmiI GGNNCC 1 cut(s) 175
BmrFI CCNGG 2 cut(s) 42, 143
BmsI GCATC 1 cut(s) 90
BpuEI CTTGAG 2 cut(s) 113, 308
BsaHI GRCGYC 2 cut(s) 174, 198
BsaI GGTCTC 1 cut(s) 326
BsaJI CCNNGG 2 cut(s) 177, 350
BsaXI ACNNNNNCTCC 2 cut(s) 368, 398
Bse118I RCCGGY 2 cut(s) 286, 367
BseBI CCWGG 2 cut(s) 42, 143
BseDI CCNNGG 2 cut(s) 177, 350
BseGI GGATG 2 cut(s) 100, 385
BseRI GAGGAG 2 cut(s) 295, 329
BseXI GCAGC 2 cut(s) 80, 358
Bsh1285I CGRYCG 1 cut(s) 210
BshFI GGCC 3 cut(s) 116, 182, 286
BshNI GGYRCC 1 cut(s) 173
BsiEI CGRYCG 1 cut(s) 210
BsiSI CCGG 3 cut(s) 171, 287, 368
BsmAI GTCTC 2 cut(s) 326, 459
BsmBI CGTCTC 1 cut(s) 459
BsnI GGCC 3 cut(s) 116, 182, 286
Bso31I GGTCTC 1 cut(s) 326
BspACI CCGC 3 cut(s) 184, 187, 251
BspANI GGCC 3 cut(s) 116, 182, 286
BspLI GGNNCC 1 cut(s) 175
BspT107I GGYRCC 1 cut(s) 173
BspTNI GGTCTC 1 cut(s) 326
BsrFI RCCGGY 2 cut(s) 286, 367
BssAI RCCGGY 2 cut(s) 286, 367
BssECI CCNNGG 2 cut(s) 177, 350
BssNI GRCGYC 2 cut(s) 174, 198
BssT1I CCWWGG 1 cut(s) 177
Bst2UI CCWGG 2 cut(s) 42, 143
Bst4CI ACNGT 1 cut(s) 208
BstACI GRCGYC 2 cut(s) 174, 198
BstC8I GCNNGC 2 cut(s) 114, 184
BstF5I GGATG 2 cut(s) 100, 385
BstH2I RGCGCY 1 cut(s) 177
BstHHI GCGC 1 cut(s) 176
BstMAI GTCTC 2 cut(s) 326, 459
BstMCI CGRYCG 1 cut(s) 210
BstMWI GCNNNNNNNGC 3 cut(s) 22, 195, 352
BstNI CCWGG 2 cut(s) 42, 143
BstSCI CCNGG 2 cut(s) 40, 141
BstV1I GCAGC 2 cut(s) 80, 358
BstXI CCANNNNNNTGG 2 cut(s) 48, 142
BsuRI GGCC 3 cut(s) 116, 182, 286
BtsCI GGATG 2 cut(s) 100, 385
Cac8I GCNNGC 2 cut(s) 114, 184
CfoI GCGC 1 cut(s) 176
Cfr10I RCCGGY 2 cut(s) 286, 367
Cfr13I GGNCC 3 cut(s) 61, 115, 181
CseI GACGC 1 cut(s) 187
Csp6I GTAC 1 cut(s) 124
CviAII CATG 1 cut(s) 136
CviJI RGCY 8 cut(s) 25, 40, 71, 116, 182, 286, 349, 355
CviKI_1 RGCY 8 cut(s) 25, 40, 71, 116, 182, 286, 349, 355
CviQI GTAC 1 cut(s) 124
DinI GGCGCC 1 cut(s) 175
DraIII CACNNNGTG 1 cut(s) 90
Eco130I CCWWGG 1 cut(s) 177
Eco31I GGTCTC 1 cut(s) 326
Eco47I GGWCC 1 cut(s) 61
EcoRII CCWGG 2 cut(s) 40, 141
EcoT14I CCWWGG 1 cut(s) 177
EgeI GGCGCC 1 cut(s) 175
EheI GGCGCC 1 cut(s) 175
ErhI CCWWGG 1 cut(s) 177
Esp3I CGTCTC 1 cut(s) 459
FaeI CATG 1 cut(s) 139
FaiI YATR 2 cut(s) 137, 191
FatI CATG 1 cut(s) 135
FauI CCCGC 1 cut(s) 191
Fnu4HI GCNGC 3 cut(s) 69, 187, 347
FokI GGATG 2 cut(s) 107, 392
Fsp4HI GCNGC 3 cut(s) 69, 187, 347
GlaI GCGC 1 cut(s) 175
GluI GCNGC 3 cut(s) 69, 187, 347
HaeII RGCGCY 1 cut(s) 177
HaeIII GGCC 3 cut(s) 116, 182, 286
HapII CCGG 3 cut(s) 171, 287, 368
HgaI GACGC 1 cut(s) 187
HhaI GCGC 1 cut(s) 176
Hin1I GRCGYC 2 cut(s) 174, 198
Hin1II CATG 1 cut(s) 139
Hin6I GCGC 1 cut(s) 174
HinP1I GCGC 1 cut(s) 174
HincII GTYRAC 1 cut(s) 292
HindII GTYRAC 1 cut(s) 292
HinfI GANTC 2 cut(s) 99, 449
HpaII CCGG 3 cut(s) 171, 287, 368
HphI GGTGA 4 cut(s) 76, 245, 434, 467
Hpy166II GTNNAC 1 cut(s) 292
Hpy188I TCNGA 3 cut(s) 30, 229, 376
Hpy188III TCNNGA 2 cut(s) 103, 202
Hpy8I GTNNAC 1 cut(s) 292
Hpy99I CGWCG 1 cut(s) 203
HpyAV CCTTC 7 cut(s) 224, 230, 256, 292, 301, 413, 455
HpyCH4III ACNGT 1 cut(s) 208
HpyF10VI GCNNNNNNNGC 3 cut(s) 22, 195, 352
Hsp92I GRCGYC 2 cut(s) 174, 198
Hsp92II CATG 1 cut(s) 139
HspAI GCGC 1 cut(s) 174
KasI GGCGCC 1 cut(s) 173
Lsp1109I GCAGC 2 cut(s) 80, 358
LweI GCATC 1 cut(s) 90
MaeIII GTNAC 1 cut(s) 202
MboII GAAGA 2 cut(s) 151, 452
Mly113I GGCGCC 1 cut(s) 174
MlyI GAGTC 1 cut(s) 108
MspI CCGG 3 cut(s) 171, 287, 368
MspR9I CCNGG 2 cut(s) 42, 143
MvaI CCWGG 2 cut(s) 42, 143
MwoI GCNNNNNNNGC 3 cut(s) 22, 195, 352
NarI GGCGCC 1 cut(s) 174
NlaIII CATG 1 cut(s) 139
NlaIV GGNNCC 1 cut(s) 175
NmeAIII GCCGAG 1 cut(s) 375
NmuCI GTSAC 1 cut(s) 202
PcsI WCGNNNNNNNCGW 1 cut(s) 207
PfeI GAWTC 1 cut(s) 449
PkrI GCNGC 3 cut(s) 70, 188, 348
PleI GAGTC 1 cut(s) 107
PluTI GGCGCC 1 cut(s) 177
PpsI GAGTC 1 cut(s) 107
Psp6I CCWGG 2 cut(s) 40, 141
PspGI CCWGG 2 cut(s) 40, 141
PspN4I GGNNCC 1 cut(s) 175
PspPI GGNCC 3 cut(s) 61, 115, 181
RsaI GTAC 1 cut(s) 125
RsaNI GTAC 1 cut(s) 124
SatI GCNGC 3 cut(s) 69, 187, 347
Sau96I GGNCC 3 cut(s) 61, 115, 181
SchI GAGTC 1 cut(s) 108
ScrFI CCNGG 2 cut(s) 42, 143
SetI ASST 7 cut(s) 129, 144, 153, 235, 241, 412, 424
SfaNI GCATC 1 cut(s) 90
SfoI GGCGCC 1 cut(s) 175
SinI GGWCC 1 cut(s) 61
SmlI CTYRAG 2 cut(s) 128, 323
SmoI CTYRAG 2 cut(s) 128, 323
SsiI CCGC 3 cut(s) 184, 187, 251
SspDI GGCGCC 1 cut(s) 173
StyD4I CCNGG 2 cut(s) 40, 141
StyI CCWWGG 1 cut(s) 177
TaaI ACNGT 1 cut(s) 208
TauI GCSGC 1 cut(s) 189
TfiI GAWTC 1 cut(s) 449
TseFI GTSAC 1 cut(s) 202
TseI GCWGC 2 cut(s) 68, 346
Tsp45I GTSAC 1 cut(s) 202
TspDTI ATGAA 4 cut(s) 152, 320, 462, 474
VpaK11BI GGWCC 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.