RchiOBHm_Chr6g0308151

Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0014820
Physical Location & Seq
Forward (+)
66245667 .. 66254674
9008 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3807 bp
ATGTCCGACTTGCTTGCCTATCTACTGAAATCGTTGGTTCAACTCATTAGGCCAGAGCTACAAGCTAGATTTGACAAAACTCATCAGGAGTTTAACAGCTTTCAAGATGTACTCAATCTCTATGAAGGAGGGATTCCGTTGCCTGAGGGTATATTAAAGGATATTGGCGACAAAATTCCTTTGCCAATGATCAAGGAAATTTTCCGAACCGACGGCGATAGGACTGCTTGGAGGACTGATGAAGAATTTGCCAGAGAAATGCTGGCTGGAGTCAACCCTGTAACCATTCGTCGCCTCCAAACGTTTCCACCAGCAAGCAAACTAGATCCAAATGTTTATGGTGATCACAGCAGTACCATAACCGAACACCACATCAAGAATAACTTGGATGGGCTAACGGTGCATCAGGCACTCAGGGACAACAAGTTATTCATATTAGACCATCATGATGCCTTCATGCCGTACCTGTGGCGGATAAACTCGACCAAAAACAAGATCTATGGCAGCAGGACACTCCTTTTCTTGAAAAGTGATGGGACTTTGAAGCCATTGGTGATTGAACTAAGCTTGCCTCATCGTAATGGAGATCAATTTGGTTGCACTAGCAAAGTATATACACCAGCTGAACATGGTGTTGAGAGCTCCATATGGCAACTGGCTAAGGCTTATGCAGCTGTAAATGACTCTGGATTTCATCCAACTTATCAGTCACTGGTATTATTACTTTACTCCTGCCAAAGAATAAGAGATTGGAGAATAAGTTCATATATATTCAACACCCATACTTTATCTTCCGGTCACAGGTTGAATACCCATGCGGTCATCGAGCCGTTTGTAATAGCCACCAACAGGCAGCTGAATGTGCTTCATCCAGTTTACAAACTTCTGCTTCCTCGCTTCCATGACACGATGCATGTAAATGCACTGGCCATGCATGTTCTCATTAATGCTGGTGGCTTTCTGGAGAGCACAGTTTTCCCAGCTCAGTATGCCATGGAACTATCAGCTGTGGTTTATAAGAGTTGGGTTTTTCCCGACCAAGCTCTCCCTGCAGATCTCATCAAGAGAGGGATTGCTATCAAGGATGCCAATTCTCCACACGGTGTTCGCCTACTGATAGAGGACTACCCATATGCTGATGATGGACTGAATATTTGGTCTGCAATAAAAACTTTGTTGGATCTGTGTACTCGGGTGATAATTGGCAAAGGAGATTTGAGGGAGAGACTGTTTCACTTGGATTGCATGTACGGAGGACCGACACAAGCACCATCAAGTCCACAAGGTCATGTTGCCCTGACATTGAGTTATGATCGGATGAATGAATTGTGGTTATGGCATAGTCGTTTGGGTCACCCATCCTTTGGTGTCATGAAGAAGTCCATGCCTTCTCTTTTTTTGGGAATAAGTGATTCTAGTCTACATTGTGAAACCTATGCTTTAGCCAAGAGTCATAGGTCTAGTTATCCTTCTAACTTTCAGTCTAGTACAATGCCTTTTGAATTAATTCATTCTGATTTATGGGGACCTTCAAGAAATTCAACTCTTTCAGGAATGCATTATTTTGTTTTATTCATTGATAACTTCACCAGATTAACTTGGGTTGTTTTGCTTAAGTCAAAAGATACAGTTTTCTCTGCCTTTACAGCCTTCCATAATCTTGGTGTTTTTTATGAAGGACAGTGCCAAACAGGACCAACGATCGATCGCTTTATCTCAAACGATCAATTGGGTTGTGAAGAGGAAGTGTCAAATGCCCCAGAAAACAATGACACTCCTGCAGAAATAGAAAAGCCAATTGCCGAACAGAGCGTTGCGAGTTCCGAAACAGAAAAGGAAATTGCTGAATCGTCTAGCCTTCGCCATGCAACTGCCGAACAAAGCATTACAAGTTCCGAAACAAAAAAGGCAATTACTGAATCGTCTACTCTTCGCCATGCGACTGCCGAACAGAGCATTGTGAGTTCCGACACAGAAAATGCAATTGCCGAATCGTCTACCCTCAAGAATATCCTAATCATTCAGTCTCTCCCTCTCCATCAGTGGTCTCCCCTGTTCAATCTTCATCTGAGTAAAGTGCAGGATGCAATGAAGGACGAGAAGTGGGCAAAAGCAATGGCTGTGGAGATGGATGCACTTGAAAAAAATCAAACGTGGGAGTTAGTATCACTACCTCCTGGAAAGAAAACAGTTGGGTGTCGATGGGTGTATACAGTGAAGCACAATTCAGATGGTTCGGTGGACAGATATAAGGCAAGGCTAGTAGCGAAGGGTTATACCCAGAAGTATAGTGTGGACTATGATGAAACTTTTGCACCAGTAGCAAAGATCAATACAATCCAGGTACTTCTGTCTCTAGCAGCTAATCTTGATTGGCCTCTGCAACAATTTGATGTAAAAAATGCCTTCTTGCATGGTGATTTGAATGAAGAAGTATACATGGACTTGCCTCCGGGATATGGTACTTCCACTGGAGTCAAGGTGGTGTGTCGTTTAAGAAAGTCCCTACATGGCCTCAAACAATCTCCAAGAGCTTGGTTTGGTCGATTCACTACATTCATGAGGAGGATTGGGTATAGGCAGAGCAATTCAGATCATACCCTATTCCTTAAGAATCAGAAGGGTAAAGTTACAGTTTTAATTATTTATGTGGATGACATGGTGGTGACAGGTAATGATCTTGAGGAGATTAAGAAGTTGCAGAGTGCATTGTCAGTAGAATTTGAAATGAAAGACTTGGGGAGTTTGAAATATTTCTTGGGAATTGAAGTTGCTAGAGGAAAAGATTGTATTATGTTGAGCCAGAGAAAGTATGTCCTTGACTTATTAGCAGAGACGGGTATGCTTGATTGCCAACCAGCTGACACACCCATTGAGCAGAACCATCGGTTAGCTGAGTATCCAGATCAAACTCATATGGAAGCTGTAGTTAGGATCTTGAGGTACTTGAAGTCTGCTCCCGGAAGAGGGTTGGTGTTTTCTAAGCATAGGCATTTGGATGTTTTGGGGTATACAGATGCAGACTGGGCAGGTTGTATAACTGATCGACGGTCCACTTCCGGATACTTTACATTTTTAGGTGGGAATTTAGTTACTTGGAAGAGTAAGAAACAGAAGGTGGTAGCCCGTTCTAGTGCTGAAGCAGAATATAGAGGAATGGCTCGTGGAGTTTACTATTGCTCCTTCTACTACAAGACTGATGATATGGTTCAAAAAGACACTGAACTCCAGTCCTGGTGGAAGGAACTTGTGGAGCAGGGTCATGGTGACAAAAAGAATGAACCCTGGTGGCCTCAAATGCATACTCGTGATGAGCTCAAACAAACATGCACTATCATCATTTGGATTGTCTCTGCGCTCCATGCAGTCGTCAACTTCGGACAATACTCTTTTGCTGGATACCTTCCAAACCGACCAACTATAAGCAGGAGATTCATGCCTGAGATAGGAACTCCGGAATATGAAGAGCTCAAGACCAACCCTGATTTGGCTTTCTTGAAAACTATTACTGCTCGGTGTCAAACTTTGATCAATGTTTCGCTGATAGAAATTTTGTCTAGGCATGCTAGTGATGAAATCTATCTGGGGCAGAGAGACACACCTGGGTGGACATCAGACACAAAAGTGTTGGAAGCCTTCGAGAGGTTTGGAAAGAAACTGGCTGAAATTGAGGCTGGAATTATACGTATGAACAATAGCGGGAAGCTGAAGAACCAAGTTAGACCGGTCAAGGCTTATACTTTGCTCTATCCTACCGGCGAAGCTGGACTTTTTGGCAATGGAATCCCCAACAGTGTCTCAATCTAA

Protein Analysis

1268

Amino Acids

143.42

Weight (kDa)

6.84

Isoelectric Point (pI)

41.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipoxygenase PF00305 2 - 233 2.2e-75 Lipoxygenase
Lipoxygenase PF00305 261 - 393 8.8e-62 Lipoxygenase
gag_pre-integrs PF13976 426 - 484 1e-11 GAG-pre-integrase domain
RVT_2 PF07727 716 - 959 1.7e-71 Reverse transcriptase (RNA-dependent DNA polymerase)
Lipoxygenase PF00305 1059 - 1251 7.6e-80 Lipoxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1017
Acc36I ACCTGC 1 cut(s) 3020
AccB7I CCANNNNNTGG 1 cut(s) 1364
AccI GTMKAC 6 cut(s) 1420, 1925, 1997, 2210, 2436, 3011
AccIII TCCGGA 2 cut(s) 3059, 3454
AciI CCGC 3 cut(s) 472, 818, 3699
AclI AACGTT 1 cut(s) 302
AclWI GGATC 3 cut(s) 320, 1188, 2942
AcoI YGGCCR 1 cut(s) 927
AcsI RAATTY 7 cut(s) 174, 198, 245, 1537, 2720, 3085, 3549
AcuI CTGAAG 2 cut(s) 3159, 3728
AdeI CACNNNGTG 1 cut(s) 1103
AfaI GTAC 9 cut(s) 111, 355, 464, 1189, 1250, 1489, 2346, 2464, 2945
AfiI CCNNNNNNNGG 9 cut(s) 801, 849, 1364, 2459, 2966, 2967, 3446, 3487, 3642
AflII CTTAAG 2 cut(s) 1613, 2609
AgeI ACCGGT 1 cut(s) 3724
AjnI CCWGG 5 cut(s) 2176, 2340, 3233, 3284, 3601
AjuI GAANNNNNNNTTGG 4 cut(s) 733, 765, 2741, 2773
AleI CACNNNNGTG 2 cut(s) 3604, 3623
AloI GAACNNNNNNTCC 2 cut(s) 745, 777
Alw21I GWGCWC 4 cut(s) 644, 971, 3318, 3471
Alw26I GTCTC 8 cut(s) 1219, 2031, 2052, 2358, 2828, 3355, 3588, 3802
AlwI GGATC 3 cut(s) 320, 1188, 2942
AlwNI CAGNNNCTG 1 cut(s) 712
Ama87I CYCGRG 1 cut(s) 1191
Aor13HI TCCGGA 2 cut(s) 3059, 3454
AoxI GGCC 5 cut(s) 50, 927, 2375, 2512, 3290
ApeKI GCWGC 4 cut(s) 504, 671, 853, 2360
ApoI RAATTY 7 cut(s) 174, 198, 245, 1537, 2720, 3085, 3549
ArsI GACNNNNNNTTYG 2 cut(s) 1137, 1169
AseI ATTAAT 2 cut(s) 945, 1505
AsiGI ACCGGT 1 cut(s) 3724
Asp700I GAANNNNTTC 3 cut(s) 760, 1506, 2753
AspLEI GCGC 1 cut(s) 3358
AspS9I GGNCC 4 cut(s) 1256, 1526, 1694, 3051
AsuC2I CCSGG 2 cut(s) 2454, 2961
AsuHPI GGTGA 8 cut(s) 353, 565, 1207, 1346, 1579, 2429, 2677, 3278
AvaI CYCGRG 1 cut(s) 1191
AvaII GGWCC 4 cut(s) 1256, 1526, 1694, 3051
AxyI CCTNAGG 1 cut(s) 144
BaeI ACNNNNGTAYC 4 cut(s) 337, 370, 2336, 2369
BalI TGGCCA 1 cut(s) 929
BanII GRGCYC 3 cut(s) 644, 3318, 3471
BauI CACGAG 2 cut(s) 3162, 3306
Bbv12I GWGCWC 4 cut(s) 644, 971, 3318, 3471
BbvI GCAGC 4 cut(s) 516, 683, 865, 2372
BceAI ACGGC 3 cut(s) 229, 445, 814
BcgI CGANNNNNNTGC 5 cut(s) 30, 206, 240, 2867, 2901
BciT130I CCWGG 5 cut(s) 2178, 2342, 3235, 3286, 3603
BciVI GTATCC 3 cut(s) 2910, 3056, 3392
BclI TGATCA 3 cut(s) 189, 343, 3528
BcnI CCSGG 2 cut(s) 2454, 2961
BcoDI GTCTC 8 cut(s) 1219, 2031, 2052, 2358, 2828, 3355, 3588, 3802
BfmI CTRYAG 3 cut(s) 1050, 1779, 2925
BfrI CTTAAG 2 cut(s) 1613, 2609
BfuAI ACCTGC 1 cut(s) 3020
BfuI GTATCC 3 cut(s) 2910, 3056, 3392
BglII AGATCT 2 cut(s) 495, 1054
BisI GCNGC 4 cut(s) 505, 672, 854, 2361
BlsI GCNGC 4 cut(s) 506, 673, 855, 2362
Bme1390I CCNGG 7 cut(s) 2178, 2342, 2454, 2961, 3235, 3286, 3603
Bme18I GGWCC 4 cut(s) 1256, 1526, 1694, 3051
BmeT110I CYCGRG 1 cut(s) 1191
BmgT120I GGNCC 4 cut(s) 1256, 1526, 1694, 3051
BmiI GGNNCC 1 cut(s) 1527
BmrFI CCNGG 7 cut(s) 2178, 2342, 2454, 2961, 3235, 3286, 3603
BmrI ACTGGG 1 cut(s) 3034
BmsI GCATC 7 cut(s) 412, 439, 900, 1075, 2074, 2122, 3007
BmuI ACTGGG 1 cut(s) 3034
BpmI CTGGAG 4 cut(s) 288, 983, 2493, 3212
Bpu10I CCTNAGC 1 cut(s) 660
BpuEI CTTGAG 4 cut(s) 1988, 2702, 2959, 3455
BpuMI CCSGG 2 cut(s) 2454, 2961
Bsa29I ATCGAT 1 cut(s) 1704
BsaAI YACGTR 1 cut(s) 3686
BsaBI GATNNNNATC 1 cut(s) 1076
BsaI GGTCTC 1 cut(s) 2052
BsaJI CCNNGG 3 cut(s) 993, 3284, 3602
BsaWI WCCGGW 4 cut(s) 794, 3059, 3454, 3724
BsaXI ACNNNNNCTCC 8 cut(s) 745, 775, 1214, 1244, 2466, 2496, 3164, 3194
Bsc4I CCNNNNNNNGG 9 cut(s) 801, 849, 1364, 2459, 2966, 2967, 3446, 3487, 3642
Bse118I RCCGGY 2 cut(s) 3724, 3755
Bse1I ACTGG 9 cut(s) 660, 717, 872, 930, 2318, 2476, 3029, 3229, 3663
Bse21I CCTNAGG 1 cut(s) 144
Bse3DI GCAATG 3 cut(s) 2094, 2121, 3784
Bse8I GATNNNNATC 1 cut(s) 1076
BseAI TCCGGA 2 cut(s) 3059, 3454
BseBI CCWGG 5 cut(s) 2178, 2342, 3235, 3286, 3603
BseCI ATCGAT 1 cut(s) 1704
BseDI CCNNGG 3 cut(s) 993, 3284, 3602
BseJI GATNNNNATC 1 cut(s) 1076
BseLI CCNNNNNNNGG 9 cut(s) 801, 849, 1364, 2459, 2966, 2967, 3446, 3487, 3642
BseMI GCAATG 3 cut(s) 2094, 2121, 3784
BseMII CTCAG 6 cut(s) 135, 427, 998, 2060, 2886, 3432
BseNI ACTGG 9 cut(s) 660, 717, 872, 930, 2318, 2476, 3029, 3229, 3663
BseRI GAGGAG 2 cut(s) 2578, 2699
BseXI GCAGC 4 cut(s) 516, 683, 865, 2372
BseYI CCCAGC 1 cut(s) 979
BsgI GTGCAG 1 cut(s) 2099
Bsh1285I CGRYCG 2 cut(s) 1704, 1708
BshFI GGCC 5 cut(s) 52, 929, 2377, 2514, 3292
BshTI ACCGGT 1 cut(s) 3724
BshVI ATCGAT 1 cut(s) 1704
BsiEI CGRYCG 2 cut(s) 1704, 1708
BsiHKAI GWGCWC 4 cut(s) 644, 971, 3318, 3471
BsiHKCI CYCGRG 1 cut(s) 1191
BsiSI CCGG 7 cut(s) 795, 2453, 2961, 3060, 3455, 3725, 3756
BslFI GGGAC 4 cut(s) 431, 550, 1539, 2488
BslI CCNNNNNNNGG 9 cut(s) 801, 849, 1364, 2459, 2966, 2967, 3446, 3487, 3642
BsmAI GTCTC 8 cut(s) 1219, 2031, 2052, 2358, 2828, 3355, 3588, 3802
BsmBI CGTCTC 1 cut(s) 2828
BsmFI GGGAC 4 cut(s) 431, 550, 1539, 2488
BsmI GAATGC 1 cut(s) 1560
BsnI GGCC 5 cut(s) 52, 929, 2377, 2514, 3292
Bso31I GGTCTC 1 cut(s) 2052
BsoBI CYCGRG 1 cut(s) 1191
Bsp1286I GDGCHC 4 cut(s) 644, 971, 3318, 3471
Bsp13I TCCGGA 2 cut(s) 3059, 3454
Bsp19I CCATGG 1 cut(s) 993
BspACI CCGC 3 cut(s) 472, 818, 3699
BspANI GGCC 5 cut(s) 52, 929, 2377, 2514, 3292
BspCNI CTCAG 6 cut(s) 136, 426, 997, 2061, 2887, 3433
BspDI ATCGAT 1 cut(s) 1704
BspEI TCCGGA 2 cut(s) 3059, 3454
BspHI TCATGA 3 cut(s) 445, 1371, 2559
BspLI GGNNCC 1 cut(s) 1527
BspMAI CTGCAG 2 cut(s) 1054, 1783
BspMI ACCTGC 1 cut(s) 3020
BspPI GGATC 3 cut(s) 320, 1188, 2942
BspQI GCTCTTC 1 cut(s) 3459
BspTI CTTAAG 2 cut(s) 1613, 2609
BspTNI GGTCTC 1 cut(s) 2052
BsrDI GCAATG 3 cut(s) 2094, 2121, 3784
BsrFI RCCGGY 2 cut(s) 3724, 3755
BsrI ACTGG 9 cut(s) 660, 717, 872, 930, 2318, 2476, 3029, 3229, 3663
BssAI RCCGGY 2 cut(s) 3724, 3755
BssECI CCNNGG 3 cut(s) 993, 3284, 3602
BssNAI GTATAC 3 cut(s) 2211, 2437, 3012
BssSI CACGAG 2 cut(s) 3162, 3306
BssT1I CCWWGG 1 cut(s) 993
Bst1107I GTATAC 3 cut(s) 2211, 2437, 3012
Bst2BI CACGAG 2 cut(s) 3162, 3306
Bst2UI CCWGG 5 cut(s) 2178, 2342, 3235, 3286, 3603
Bst6I CTCTTC 5 cut(s) 1734, 1935, 2959, 3095, 3459
BstAFI CTTAAG 2 cut(s) 1613, 2609
BstBAI YACGTR 1 cut(s) 3686
BstC8I GCNNGC 5 cut(s) 15, 264, 316, 569, 3564
BstDEI CTNAG 9 cut(s) 144, 413, 563, 660, 984, 2069, 2895, 2982, 3441
BstDSI CCRYGG 1 cut(s) 993
BstEII GGTNACC 1 cut(s) 1352
BstENI CCTNNNNNAGG 2 cut(s) 3444, 3640
BstHHI GCGC 1 cut(s) 3358
BstMAI GTCTC 8 cut(s) 1219, 2031, 2052, 2358, 2828, 3355, 3588, 3802
BstMCI CGRYCG 2 cut(s) 1704, 1708
BstNI CCWGG 5 cut(s) 2178, 2342, 3235, 3286, 3603
BstNSI RCATGY 5 cut(s) 917, 938, 1249, 3330, 3566
BstPI GGTNACC 1 cut(s) 1352
BstSCI CCNGG 7 cut(s) 2176, 2340, 2452, 2959, 3233, 3284, 3601
BstSFI CTRYAG 3 cut(s) 1050, 1779, 2925
BstSNI TACGTA 1 cut(s) 3686
BstV1I GCAGC 4 cut(s) 516, 683, 865, 2372
BstX2I RGATCY 5 cut(s) 325, 495, 1054, 1180, 2934
BstXI CCANNNNNNTGG 2 cut(s) 1661, 2535
BstYI RGATCY 5 cut(s) 325, 495, 1054, 1180, 2934
BstZ17I GTATAC 3 cut(s) 2211, 2437, 3012
Bsu15I ATCGAT 1 cut(s) 1704
Bsu36I CCTNAGG 1 cut(s) 144
BsuI GTATCC 3 cut(s) 2910, 3056, 3392
BsuRI GGCC 5 cut(s) 52, 929, 2377, 2514, 3292
BsuTUI ATCGAT 1 cut(s) 1704
BtgI CCRYGG 1 cut(s) 993
BtsIMutI CAGTG 8 cut(s) 710, 923, 1688, 2048, 2220, 2469, 3219, 3799
BveI ACCTGC 1 cut(s) 3020
Cac8I GCNNGC 5 cut(s) 15, 264, 316, 569, 3564
CaiI CAGNNNCTG 1 cut(s) 712
CciI TCATGA 3 cut(s) 445, 1371, 2559
CfoI GCGC 1 cut(s) 3358
Cfr10I RCCGGY 2 cut(s) 3724, 3755
Cfr13I GGNCC 4 cut(s) 1256, 1526, 1694, 3051
ClaI ATCGAT 1 cut(s) 1704
Csp6I GTAC 9 cut(s) 110, 354, 463, 1188, 1249, 1488, 2345, 2463, 2944
CspAI ACCGGT 1 cut(s) 3724
CspCI CAANNNNNGTGG 2 cut(s) 2103, 2138
CviQI GTAC 9 cut(s) 110, 354, 463, 1188, 1249, 1488, 2345, 2463, 2944
DdeI CTNAG 9 cut(s) 144, 413, 563, 660, 984, 2069, 2895, 2982, 3441
DraIII CACNNNGTG 1 cut(s) 1103
EaeI YGGCCR 1 cut(s) 927
Eam1104I CTCTTC 5 cut(s) 1734, 1935, 2959, 3095, 3459
EarI CTCTTC 5 cut(s) 1734, 1935, 2959, 3095, 3459
EciI GGCGGA 1 cut(s) 487
Ecl136II GAGCTC 3 cut(s) 642, 3316, 3469
Eco105I TACGTA 1 cut(s) 3686
Eco130I CCWWGG 1 cut(s) 993
Eco24I GRGCYC 3 cut(s) 644, 3318, 3471
Eco31I GGTCTC 1 cut(s) 2052
Eco47I GGWCC 4 cut(s) 1256, 1526, 1694, 3051
Eco53kI GAGCTC 3 cut(s) 642, 3316, 3469
Eco57I CTGAAG 2 cut(s) 3159, 3728
Eco81I CCTNAGG 1 cut(s) 144
Eco88I CYCGRG 1 cut(s) 1191
Eco91I GGTNACC 1 cut(s) 1352
EcoICRI GAGCTC 3 cut(s) 642, 3316, 3469
EcoNI CCTNNNNNAGG 2 cut(s) 3444, 3640
EcoO109I RGGNCCY 1 cut(s) 1526
EcoO65I GGTNACC 1 cut(s) 1352
EcoRII CCWGG 5 cut(s) 2176, 2340, 3233, 3284, 3601
EcoT14I CCWWGG 1 cut(s) 993
EcoT22I ATGCAT 4 cut(s) 915, 936, 1560, 3303
EcoT38I GRGCYC 3 cut(s) 644, 3318, 3471
ErhI CCWWGG 1 cut(s) 993
Esp3I CGTCTC 1 cut(s) 2828
FalI AAGNNNNNCTT 4 cut(s) 368, 400, 3753, 3785
FaqI GGGAC 4 cut(s) 431, 550, 1539, 2488
FauI CCCGC 1 cut(s) 3692
FauNDI CATATG 3 cut(s) 647, 1132, 2916
FbaI TGATCA 3 cut(s) 189, 343, 3528
FblI GTMKAC 6 cut(s) 1420, 1925, 1997, 2210, 2436, 3011
Fnu4HI GCNGC 4 cut(s) 505, 672, 854, 2361
FriOI GRGCYC 3 cut(s) 644, 3318, 3471
Fsp4HI GCNGC 4 cut(s) 505, 672, 854, 2361
GlaI GCGC 1 cut(s) 3357
GluI GCNGC 4 cut(s) 505, 672, 854, 2361
GsaI CCCAGC 1 cut(s) 983
GsuI CTGGAG 4 cut(s) 288, 983, 2493, 3212
HaeIII GGCC 5 cut(s) 52, 929, 2377, 2514, 3292
HapII CCGG 7 cut(s) 795, 2453, 2961, 3060, 3455, 3725, 3756
HhaI GCGC 1 cut(s) 3358
Hin6I GCGC 1 cut(s) 3356
HinP1I GCGC 1 cut(s) 3356
HincII GTYRAC 2 cut(s) 274, 3373
HindII GTYRAC 2 cut(s) 274, 3373
HindIII AAGCTT 1 cut(s) 565
HpaII CCGG 7 cut(s) 795, 2453, 2961, 3060, 3455, 3725, 3756
HphI GGTGA 8 cut(s) 353, 565, 1207, 1346, 1579, 2429, 2677, 3278
Hpy99I CGWCG 3 cut(s) 215, 294, 3051
HpyCH4IV ACGT 3 cut(s) 302, 2153, 3685
HpyF3I CTNAG 9 cut(s) 144, 413, 563, 660, 984, 2069, 2895, 2982, 3441
HpySE526I ACGT 3 cut(s) 302, 2153, 3685
HspAI GCGC 1 cut(s) 3356
Kpn2I TCCGGA 2 cut(s) 3059, 3454
Ksp22I TGATCA 3 cut(s) 189, 343, 3528
LguI GCTCTTC 1 cut(s) 3459
LmnI GCTCC 5 cut(s) 647, 2962, 3185, 3253, 3363
Lsp1109I GCAGC 4 cut(s) 516, 683, 865, 2372
LweI GCATC 7 cut(s) 412, 439, 900, 1075, 2074, 2122, 3007
MaeII ACGT 3 cut(s) 302, 2153, 3685
MaeIII GTNAC 8 cut(s) 280, 708, 797, 1352, 2629, 2665, 3091, 3266
MfeI CAATTG 3 cut(s) 1727, 1797, 1983
MflI RGATCY 5 cut(s) 325, 495, 1054, 1180, 2934
MhlI GDGCHC 4 cut(s) 644, 971, 3318, 3471
MlsI TGGCCA 1 cut(s) 929
MluNI TGGCCA 1 cut(s) 929
MlyI GAGTC 4 cut(s) 279, 677, 1459, 2484
MmeI TCCRAC 5 cut(s) 30, 722, 1158, 1992, 3609
Mox20I TGGCCA 1 cut(s) 929
Mph1103I ATGCAT 4 cut(s) 915, 936, 1560, 3303
MroI TCCGGA 2 cut(s) 3059, 3454
MroXI GAANNNNTTC 3 cut(s) 760, 1506, 2753
MscI TGGCCA 1 cut(s) 929
MslI CAYNNNNRTG 9 cut(s) 447, 579, 918, 2663, 2997, 3306, 3567, 3604, 3623
Msp20I TGGCCA 1 cut(s) 929
MspA1I CMGCKG 5 cut(s) 623, 674, 856, 1007, 2861
MspCI CTTAAG 2 cut(s) 1613, 2609
MspI CCGG 7 cut(s) 795, 2453, 2961, 3060, 3455, 3725, 3756
MspR9I CCNGG 7 cut(s) 2178, 2342, 2454, 2961, 3235, 3286, 3603
MunI CAATTG 3 cut(s) 1727, 1797, 1983
Mva1269I GAATGC 1 cut(s) 1560
MvaI CCWGG 5 cut(s) 2178, 2342, 3235, 3286, 3603
NciI CCSGG 2 cut(s) 2454, 2961
NcoI CCATGG 1 cut(s) 993
NdeI CATATG 3 cut(s) 647, 1132, 2916
NlaIV GGNNCC 1 cut(s) 1527
NmuCI GTSAC 5 cut(s) 708, 797, 1352, 2665, 3266
NsiI ATGCAT 4 cut(s) 915, 936, 1560, 3303
NspI RCATGY 5 cut(s) 917, 938, 1249, 3330, 3566
OliI CACNNNNGTG 2 cut(s) 3604, 3623
PaeI GCATGC 1 cut(s) 3566
PagI TCATGA 3 cut(s) 445, 1371, 2559
PciSI GCTCTTC 1 cut(s) 3459
PctI GAATGC 1 cut(s) 1560
PdmI GAANNNNTTC 3 cut(s) 760, 1506, 2753
PfeI GAWTC 9 cut(s) 133, 1412, 1847, 1919, 1991, 2547, 2614, 3432, 3783
PflMI CCANNNNNTGG 1 cut(s) 1364
PfoI TCCNGGA 3 cut(s) 2176, 2452, 2959
PinAI ACCGGT 1 cut(s) 3724
PkrI GCNGC 4 cut(s) 506, 673, 855, 2362
Ple19I CGATCG 2 cut(s) 1704, 1708
PleI GAGTC 4 cut(s) 278, 677, 1458, 2483
PpsI GAGTC 4 cut(s) 278, 677, 1458, 2483
Ppu21I YACGTR 1 cut(s) 3686
PpuMI RGGWCCY 1 cut(s) 1526
PshBI ATTAAT 2 cut(s) 945, 1505
PsiI TTATAA 1 cut(s) 1017
Psp124BI GAGCTC 3 cut(s) 644, 3318, 3471
Psp1406I AACGTT 1 cut(s) 302
Psp5II RGGWCCY 1 cut(s) 1526
Psp6I CCWGG 5 cut(s) 2176, 2340, 3233, 3284, 3601
PspEI GGTNACC 1 cut(s) 1352
PspFI CCCAGC 1 cut(s) 979
PspGI CCWGG 5 cut(s) 2176, 2340, 3233, 3284, 3601
PspN4I GGNNCC 1 cut(s) 1527
PspPI GGNCC 4 cut(s) 1256, 1526, 1694, 3051
PspPPI RGGWCCY 1 cut(s) 1526
PstI CTGCAG 2 cut(s) 1054, 1783
PstNI CAGNNNCTG 1 cut(s) 712
PsuI RGATCY 5 cut(s) 325, 495, 1054, 1180, 2934
PvuI CGATCG 2 cut(s) 1704, 1708
PvuII CAGCTG 5 cut(s) 623, 674, 856, 1007, 2861
RsaI GTAC 9 cut(s) 111, 355, 464, 1189, 1250, 1489, 2346, 2464, 2945
RsaNI GTAC 9 cut(s) 110, 354, 463, 1188, 1249, 1488, 2345, 2463, 2944
RseI CAYNNNNRTG 9 cut(s) 447, 579, 918, 2663, 2997, 3306, 3567, 3604, 3623
SacI GAGCTC 3 cut(s) 644, 3318, 3471
SapI GCTCTTC 1 cut(s) 3459
SatI GCNGC 4 cut(s) 505, 672, 854, 2361
Sau96I GGNCC 4 cut(s) 1256, 1526, 1694, 3051
SchI GAGTC 4 cut(s) 279, 677, 1459, 2484
ScrFI CCNGG 7 cut(s) 2178, 2342, 2454, 2961, 3235, 3286, 3603
SduI GDGCHC 4 cut(s) 644, 971, 3318, 3471
SfaNI GCATC 7 cut(s) 412, 439, 900, 1075, 2074, 2122, 3007
SfcI CTRYAG 3 cut(s) 1050, 1779, 2925
SinI GGWCC 4 cut(s) 1256, 1526, 1694, 3051
SmiMI CAYNNNNRTG 9 cut(s) 447, 579, 918, 2663, 2997, 3306, 3567, 3604, 3623
SmlI CTYRAG 6 cut(s) 1613, 2003, 2609, 2681, 2938, 3470
SmoI CTYRAG 6 cut(s) 1613, 2003, 2609, 2681, 2938, 3470
SnaBI TACGTA 1 cut(s) 3686
SphI GCATGC 1 cut(s) 3566
SsiI CCGC 3 cut(s) 472, 818, 3699
SspI AATATT 2 cut(s) 1153, 2753
SstI GAGCTC 3 cut(s) 644, 3318, 3471
StyD4I CCNGG 7 cut(s) 2176, 2340, 2452, 2959, 3233, 3284, 3601
StyI CCWWGG 1 cut(s) 993
TaiI ACGT 3 cut(s) 305, 2156, 3688
TaqI TCGA 7 cut(s) 482, 825, 1704, 2200, 2545, 3046, 3639
TaqII GACCGA 1 cut(s) 1273
TatI WGTACW 3 cut(s) 109, 1187, 1487
TfiI GAWTC 9 cut(s) 133, 1412, 1847, 1919, 1991, 2547, 2614, 3432, 3783
TscAI CASTG 8 cut(s) 717, 930, 1688, 2048, 2220, 2476, 3226, 3799
TseFI GTSAC 5 cut(s) 708, 797, 1352, 2665, 3266
TseI GCWGC 4 cut(s) 504, 671, 853, 2360
Tsp45I GTSAC 5 cut(s) 708, 797, 1352, 2665, 3266
TspGWI ACGGA 2 cut(s) 126, 1266
TspRI CASTG 8 cut(s) 717, 930, 1688, 2048, 2220, 2476, 3226, 3799
Van91I CCANNNNNTGG 1 cut(s) 1364
Vha464I CTTAAG 2 cut(s) 1613, 2609
VpaK11BI GGWCC 4 cut(s) 1256, 1526, 1694, 3051
VspI ATTAAT 2 cut(s) 945, 1505
XagI CCTNNNNNAGG 2 cut(s) 3444, 3640
XapI RAATTY 7 cut(s) 174, 198, 245, 1537, 2720, 3085, 3549
XceI RCATGY 5 cut(s) 917, 938, 1249, 3330, 3566
XcmI CCANNNNNNNNNTGG 3 cut(s) 259, 652, 3484
XmiI GTMKAC 6 cut(s) 1420, 1925, 1997, 2210, 2436, 3011
XmnI GAANNNNTTC 3 cut(s) 760, 1506, 2753
Zsp2I ATGCAT 4 cut(s) 915, 936, 1560, 3303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.