RchiOBHm_Chr5g0011471

WD domain, G-beta repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0008398
Physical Location & Seq
Reverse (-)
7605077 .. 7606781
1705 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 600 bp
ATGCTTCAAGCAAGGAATGAAACTGTGGTATATGTGTCATCGGGGAGGAAATTTAAGAGCTTTGATGTGCGTCTGGCCGGTAAGTCATCAAATCTCATTGTCTCTGTGTTTGTTTGTTTCCATTTTTGGAAGGGGAGTTCTTGCTCATGGAATGTGCTGGAGAGTTTTGAGTATAATAAAGAGAAGATAAATCAGATTGCATGCAGCTCTAAGGCTTCTTTTCTTGCTGCTGCCGATGATGGTGGTGATATTAAGATCGTTGACATTCGCCGGGAGCGCATTTACAGAACATTAAGAGCTGATGCTTGCCATACAAGTAGGTACTTTCAAATGGCTTGTAAACCTAGAATCTTCCATCCCATTGCATTCTGTCTATCCTGGAATTCTTTGCATTGTATCCACCTTGATCGTATAAGTAGCAGCATGCAATTTCTTCCTTTGAGACCTTCGGAAGTTATTACTGGAGGTCTTGATCCGAAGCTAGTGATGTGGGACTTTTCGAAGGGGCGCCCAAACAAGATATTAAATTTTGGCAAATATCCATCTTCTCTGCCATTTAACTTGTTTGATATAACTAGAATGATATTTAATCACAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

199

Amino Acids

22.86

Weight (kDa)

9.53

Isoelectric Point (pI)

40.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 31 - 117 4.8e-06 CDC20/Fizzy WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 507
AclWI GGATC 1 cut(s) 467
AcoI YGGCCR 1 cut(s) 75
AcsI RAATTY 3 cut(s) 50, 382, 526
AcyI GRCGYC 1 cut(s) 508
AfaI GTAC 1 cut(s) 323
AgsI TTSAA 2 cut(s) 8, 329
AjnI CCWGG 1 cut(s) 377
AloI GAACNNNNNNTCC 2 cut(s) 121, 153
AluBI AGCT 4 cut(s) 60, 207, 299, 481
AluI AGCT 4 cut(s) 60, 207, 299, 481
Alw26I GTCTC 2 cut(s) 106, 436
AlwI GGATC 1 cut(s) 467
AoxI GGCC 1 cut(s) 75
ApeKI GCWGC 4 cut(s) 204, 227, 230, 420
ApoI RAATTY 3 cut(s) 50, 382, 526
AspLEI GCGC 2 cut(s) 279, 510
AsuC2I CCSGG 1 cut(s) 272
AsuHPI GGTGA 1 cut(s) 257
AsuII TTCGAA 1 cut(s) 500
BanI GGYRCC 1 cut(s) 507
BbvI GCAGC 4 cut(s) 214, 216, 217, 432
BccI CCATC 3 cut(s) 233, 363, 550
BciT130I CCWGG 1 cut(s) 379
BciVI GTATCC 1 cut(s) 407
BcnI CCSGG 1 cut(s) 272
BcoDI GTCTC 2 cut(s) 106, 436
BfaI CTAG 3 cut(s) 345, 482, 576
BfoI RGCGCY 1 cut(s) 511
BfuI GTATCC 1 cut(s) 407
BisI GCNGC 4 cut(s) 205, 228, 231, 421
BlsI GCNGC 4 cut(s) 206, 229, 232, 422
Bme1390I CCNGG 2 cut(s) 272, 379
BmiI GGNNCC 1 cut(s) 509
BmrFI CCNGG 2 cut(s) 272, 379
BmsI GCATC 1 cut(s) 292
BpmI CTGGAG 2 cut(s) 179, 483
Bpu14I TTCGAA 1 cut(s) 500
BpuMI CCSGG 1 cut(s) 272
BsaHI GRCGYC 1 cut(s) 508
BsaI GGTCTC 1 cut(s) 436
Bse118I RCCGGY 1 cut(s) 77
Bse1I ACTGG 1 cut(s) 466
Bse3DI GCAATG 1 cut(s) 360
BseBI CCWGG 1 cut(s) 379
BseGI GGATG 1 cut(s) 355
BseMI GCAATG 1 cut(s) 360
BseNI ACTGG 1 cut(s) 466
BseXI GCAGC 4 cut(s) 214, 216, 217, 432
BshFI GGCC 1 cut(s) 77
BshNI GGYRCC 1 cut(s) 507
BsiSI CCGG 2 cut(s) 78, 271
BslFI GGGAC 1 cut(s) 506
BsmAI GTCTC 2 cut(s) 106, 436
BsmFI GGGAC 1 cut(s) 506
BsmI GAATGC 1 cut(s) 365
BsnI GGCC 1 cut(s) 77
Bso31I GGTCTC 1 cut(s) 436
Bsp119I TTCGAA 1 cut(s) 500
Bsp143I GATC 3 cut(s) 255, 406, 472
BspANI GGCC 1 cut(s) 77
BspLI GGNNCC 1 cut(s) 509
BspPI GGATC 1 cut(s) 467
BspT104I TTCGAA 1 cut(s) 500
BspT107I GGYRCC 1 cut(s) 507
BspTNI GGTCTC 1 cut(s) 436
BsrDI GCAATG 1 cut(s) 360
BsrFI RCCGGY 1 cut(s) 77
BsrI ACTGG 1 cut(s) 466
BssAI RCCGGY 1 cut(s) 77
BssMI GATC 3 cut(s) 255, 406, 472
BssNI GRCGYC 1 cut(s) 508
Bst2UI CCWGG 1 cut(s) 379
Bst4CI ACNGT 1 cut(s) 25
BstACI GRCGYC 1 cut(s) 508
BstBI TTCGAA 1 cut(s) 500
BstC8I GCNNGC 3 cut(s) 202, 307, 425
BstDEI CTNAG 1 cut(s) 210
BstF5I GGATG 1 cut(s) 355
BstH2I RGCGCY 1 cut(s) 511
BstHHI GCGC 2 cut(s) 279, 510
BstKTI GATC 3 cut(s) 258, 409, 475
BstMAI GTCTC 2 cut(s) 106, 436
BstMBI GATC 3 cut(s) 255, 406, 472
BstMWI GCNNNNNNNGC 1 cut(s) 276
BstNI CCWGG 1 cut(s) 379
BstNSI RCATGY 2 cut(s) 204, 427
BstSCI CCNGG 2 cut(s) 270, 377
BstV1I GCAGC 4 cut(s) 214, 216, 217, 432
BsuI GTATCC 1 cut(s) 407
BsuRI GGCC 1 cut(s) 77
BtsCI GGATG 1 cut(s) 355
Cac8I GCNNGC 3 cut(s) 202, 307, 425
CfoI GCGC 2 cut(s) 279, 510
Cfr10I RCCGGY 1 cut(s) 77
CseI GACGC 1 cut(s) 59
Csp6I GTAC 1 cut(s) 322
CviAII CATG 3 cut(s) 147, 201, 424
CviJI RGCY 7 cut(s) 60, 77, 207, 215, 299, 335, 481
CviKI_1 RGCY 7 cut(s) 60, 77, 207, 215, 299, 335, 481
CviQI GTAC 1 cut(s) 322
DdeI CTNAG 1 cut(s) 210
DinI GGCGCC 1 cut(s) 509
DpnI GATC 3 cut(s) 257, 408, 474
DpnII GATC 3 cut(s) 255, 406, 472
EaeI YGGCCR 1 cut(s) 75
Eco31I GGTCTC 1 cut(s) 436
EcoRI GAATTC 1 cut(s) 382
EcoRII CCWGG 1 cut(s) 377
EgeI GGCGCC 1 cut(s) 509
EheI GGCGCC 1 cut(s) 509
FaeI CATG 3 cut(s) 150, 204, 427
FaiI YATR 9 cut(s) 31, 33, 148, 174, 202, 312, 413, 425, 572
FaqI GGGAC 1 cut(s) 506
FatI CATG 3 cut(s) 146, 200, 423
Fnu4HI GCNGC 4 cut(s) 205, 228, 231, 421
FokI GGATG 1 cut(s) 342
Fsp4HI GCNGC 4 cut(s) 205, 228, 231, 421
FspBI CTAG 3 cut(s) 345, 482, 576
GlaI GCGC 2 cut(s) 278, 509
GluI GCNGC 4 cut(s) 205, 228, 231, 421
GsuI CTGGAG 2 cut(s) 179, 483
HaeII RGCGCY 1 cut(s) 511
HaeIII GGCC 1 cut(s) 77
HapII CCGG 2 cut(s) 78, 271
HgaI GACGC 1 cut(s) 59
HhaI GCGC 2 cut(s) 279, 510
Hin1I GRCGYC 1 cut(s) 508
Hin1II CATG 3 cut(s) 150, 204, 427
Hin6I GCGC 2 cut(s) 277, 508
HinP1I GCGC 2 cut(s) 277, 508
HincII GTYRAC 1 cut(s) 262
HindII GTYRAC 1 cut(s) 262
HinfI GANTC 1 cut(s) 348
HpaII CCGG 2 cut(s) 78, 271
HphI GGTGA 1 cut(s) 257
Hpy166II GTNNAC 2 cut(s) 262, 341
Hpy188I TCNGA 3 cut(s) 195, 451, 477
Hpy188III TCNNGA 1 cut(s) 470
Hpy8I GTNNAC 2 cut(s) 262, 341
HpyAV CCTTC 3 cut(s) 124, 456, 496
HpyCH4III ACNGT 1 cut(s) 25
HpyCH4V TGCA 5 cut(s) 200, 204, 365, 391, 427
HpyF10VI GCNNNNNNNGC 1 cut(s) 276
HpyF3I CTNAG 1 cut(s) 210
Hsp92I GRCGYC 1 cut(s) 508
Hsp92II CATG 3 cut(s) 150, 204, 427
HspAI GCGC 2 cut(s) 277, 508
KasI GGCGCC 1 cut(s) 507
Kzo9I GATC 3 cut(s) 255, 406, 472
LmnI GCTCC 1 cut(s) 274
LpnPI CCDG 7 cut(s) 59, 91, 143, 284, 364, 391, 447
Lsp1109I GCAGC 4 cut(s) 214, 216, 217, 432
LweI GCATC 1 cut(s) 292
MaeI CTAG 3 cut(s) 345, 482, 576
MalI GATC 3 cut(s) 257, 408, 474
MboI GATC 3 cut(s) 255, 406, 472
MboII GAAGA 4 cut(s) 196, 343, 425, 537
MluCI AATT 5 cut(s) 50, 382, 428, 526, 595
Mly113I GGCGCC 1 cut(s) 508
MnlI CCTC 2 cut(s) 39, 458
MseI TTAA 6 cut(s) 54, 252, 293, 524, 558, 588
MspI CCGG 2 cut(s) 78, 271
MspR9I CCNGG 2 cut(s) 272, 379
Mva1269I GAATGC 1 cut(s) 365
MvaI CCWGG 1 cut(s) 379
MwoI GCNNNNNNNGC 1 cut(s) 276
NarI GGCGCC 1 cut(s) 508
NciI CCSGG 1 cut(s) 272
NdeII GATC 3 cut(s) 255, 406, 472
NlaIII CATG 3 cut(s) 150, 204, 427
NlaIV GGNNCC 1 cut(s) 509
NspI RCATGY 2 cut(s) 204, 427
NspV TTCGAA 1 cut(s) 500
PaeI GCATGC 2 cut(s) 204, 427
PctI GAATGC 1 cut(s) 365
PfeI GAWTC 1 cut(s) 348
PfoI TCCNGGA 1 cut(s) 377
PkrI GCNGC 4 cut(s) 206, 229, 232, 422
PluTI GGCGCC 1 cut(s) 511
Psp6I CCWGG 1 cut(s) 377
PspGI CCWGG 1 cut(s) 377
PspN4I GGNNCC 1 cut(s) 509
RsaI GTAC 1 cut(s) 323
RsaNI GTAC 1 cut(s) 322
SaqAI TTAA 6 cut(s) 54, 252, 293, 524, 558, 588
SatI GCNGC 4 cut(s) 205, 228, 231, 421
Sau3AI GATC 3 cut(s) 255, 406, 472
ScrFI CCNGG 2 cut(s) 272, 379
SetI ASST 9 cut(s) 62, 209, 301, 323, 346, 405, 448, 469, 483
SfaNI GCATC 1 cut(s) 292
SfoI GGCGCC 1 cut(s) 509
SfuI TTCGAA 1 cut(s) 500
SphI GCATGC 2 cut(s) 204, 427
Sse9I AATT 5 cut(s) 50, 382, 428, 526, 595
SspDI GGCGCC 1 cut(s) 507
SspMI CTAG 3 cut(s) 345, 482, 576
StyD4I CCNGG 2 cut(s) 270, 377
TaaI ACNGT 1 cut(s) 25
TaqI TCGA 1 cut(s) 500
TasI AATT 5 cut(s) 50, 382, 428, 526, 595
TfiI GAWTC 1 cut(s) 348
Tru1I TTAA 6 cut(s) 54, 252, 293, 524, 558, 588
Tru9I TTAA 6 cut(s) 54, 252, 293, 524, 558, 588
TseI GCWGC 4 cut(s) 204, 227, 230, 420
TspDTI ATGAA 1 cut(s) 33
XapI RAATTY 3 cut(s) 50, 382, 526
XceI RCATGY 2 cut(s) 204, 427
XspI CTAG 3 cut(s) 345, 482, 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.