RchiOBHm_Chr4g0391351

Domain of unknown function (DUF296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0010554
Physical Location & Seq
Reverse (-)
6805418 .. 6807768
2351 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 945 bp
ATGTACGCCAGAGAAGAACTGCCTGGAGAAGGGGTTGAAGTGATTGCGGCTGATGCTCCCTCGGAGTACCAGATGGCTCCTAGGGCGGTGGAGCACAAGGACAAGGCGGCTACTGGTTCAGGAGAGGGGACGGGTGAGCCAAAGAAGAGAGGGAGAGGGAGGCCTCCACAGAAGAATGTAGGGGAAGGGCAGTCCTCCTCAGCCACGGGGAGAAAGGAGAAGGGCCAAGGAAGCAGCGGCGGGGGTGGAGGAGGTGGAAGTGGAGGTGCCGGTGGCAGTGGCAGTGGGGGCGGAGGAGGAGGAGGAGGAGGAGGCGGTGGGGGCGGGGGCGGCGGCCAGGGCAATCCCGGTGCAGCTTATAATGGAAGAAGCTTCACGCCCCACCTCATCTCTGTCAATGCTGGGGAGAATGTCTGCGGGAGCGTTATGAGATATTTCCATGAAAGACCACGAGCAATCTGCGTGATTTCTGCAAGTGGAGTAATCTCTAGTGTTAATCTGCGCCAACAGGATGACGAGGCCATCACATATGAGGGACGTTTCGAAATCCTGAGCTTGTCTGGATGCCTTCTGCCCCATAACGGGGCCTGGCGTGGTGCAATGAGTGTCTCTCTGGCAGGCGCCAACGGTGCAATTTTAGGGGGAGGCATTAATGGAATTCTGAGAGCTGCCACTCCTGTGCAAGTTGTGGTAGGTAGTTTCCTAGCTGGTGATCCGAAAGGAAGGGGTAAGAAGAAGGGGGATGCTGAACCACCTGATACTGAGCCTCCGCCTCCGCCTCCGCCTCCCCCTCCGCCACCGCCGTCGCCTCCACCGCCAGCACCAGAACCGCCACCTGCACCAGGACCACCACCAGCACCACCACCAGCAGCAGCACCACCACCACAAGAGCCCATGGGATTCTGGGGGATATACGATAATAGCTGGCCGAGAATGGAATGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

314

Amino Acids

31.14

Weight (kDa)

6.45

Isoelectric Point (pI)

58.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 127 - 234 2.6e-21 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 360
AarI CACCTGC 1 cut(s) 844
Acc36I ACCTGC 1 cut(s) 844
AccB1I GGYRCC 2 cut(s) 266, 620
AclWI GGATC 1 cut(s) 707
AcoI YGGCCR 2 cut(s) 334, 926
AcsI RAATTY 1 cut(s) 657
AcyI GRCGYC 1 cut(s) 621
AfaI GTAC 2 cut(s) 5, 68
AfiI CCNNNNNNNGG 5 cut(s) 29, 581, 582, 583, 842
AgsI TTSAA 1 cut(s) 38
AjnI CCWGG 4 cut(s) 22, 336, 587, 841
AleI CACNNNNGTG 1 cut(s) 677
AluBI AGCT 6 cut(s) 356, 372, 555, 668, 707, 924
AluI AGCT 6 cut(s) 356, 372, 555, 668, 707, 924
Alw21I GWGCWC 1 cut(s) 96
Alw26I GTCTC 1 cut(s) 613
AlwI GGATC 1 cut(s) 707
AoxI GGCC 6 cut(s) 161, 223, 334, 519, 585, 926
ApeKI GCWGC 5 cut(s) 234, 353, 668, 869, 872
ApoI RAATTY 1 cut(s) 657
AseI ATTAAT 1 cut(s) 651
AspA2I CCTAGG 1 cut(s) 80
AspLEI GCGC 2 cut(s) 504, 623
AspS9I GGNCC 3 cut(s) 223, 585, 845
AsuC2I CCSGG 1 cut(s) 348
AsuHPI GGTGA 2 cut(s) 146, 722
AsuII TTCGAA 1 cut(s) 543
AvaII GGWCC 1 cut(s) 845
AvrII CCTAGG 1 cut(s) 80
BanI GGYRCC 2 cut(s) 266, 620
BanII GRGCYC 1 cut(s) 894
BauI CACGAG 1 cut(s) 450
Bbv12I GWGCWC 1 cut(s) 96
BbvCI CCTCAGC 1 cut(s) 199
BbvI GCAGC 5 cut(s) 246, 365, 655, 881, 884
BccI CCATC 2 cut(s) 67, 530
BceAI ACGGC 1 cut(s) 787
BcgI CGANNNNNNTGC 2 cut(s) 441, 475
BciT130I CCWGG 4 cut(s) 24, 338, 589, 843
BcnI CCSGG 1 cut(s) 348
BcoDI GTCTC 1 cut(s) 613
BfaI CTAG 3 cut(s) 81, 489, 704
BfoI RGCGCY 1 cut(s) 624
BfuAI ACCTGC 1 cut(s) 844
BlnI CCTAGG 1 cut(s) 80
Bme1390I CCNGG 5 cut(s) 24, 338, 348, 589, 843
Bme18I GGWCC 1 cut(s) 845
BmgT120I GGNCC 3 cut(s) 223, 585, 845
BmiI GGNNCC 4 cut(s) 78, 268, 586, 622
BmrFI CCNGG 5 cut(s) 24, 338, 348, 589, 843
BmsI GCATC 3 cut(s) 43, 554, 733
BplI GAGNNNNNCTC 2 cut(s) 595, 627
BpmI CTGGAG 1 cut(s) 45
Bpu10I CCTNAGC 2 cut(s) 199, 551
Bpu14I TTCGAA 1 cut(s) 543
BpuMI CCSGG 1 cut(s) 348
BsaHI GRCGYC 1 cut(s) 621
BsaJI CCNNGG 6 cut(s) 60, 80, 204, 226, 337, 894
BsaXI ACNNNNNCTCC 4 cut(s) 18, 48, 751, 781
Bsc4I CCNNNNNNNGG 5 cut(s) 29, 581, 582, 583, 842
Bse118I RCCGGY 1 cut(s) 269
Bse1I ACTGG 1 cut(s) 118
Bse3DI GCAATG 1 cut(s) 606
BseBI CCWGG 4 cut(s) 24, 338, 589, 843
BseDI CCNNGG 6 cut(s) 60, 80, 204, 226, 337, 894
BseGI GGATG 3 cut(s) 517, 569, 748
BseLI CCNNNNNNNGG 5 cut(s) 29, 581, 582, 583, 842
BseMI GCAATG 1 cut(s) 606
BseMII CTCAG 4 cut(s) 213, 542, 653, 753
BseNI ACTGG 1 cut(s) 118
BseRI GAGGAG 8 cut(s) 187, 264, 309, 312, 315, 318, 321, 324
BseXI GCAGC 5 cut(s) 246, 365, 655, 881, 884
BseYI CCCAGC 1 cut(s) 401
BsgI GTGCAG 2 cut(s) 372, 822
BshFI GGCC 6 cut(s) 163, 225, 336, 521, 587, 928
BshNI GGYRCC 2 cut(s) 266, 620
BsiHKAI GWGCWC 1 cut(s) 96
BsiSI CCGG 2 cut(s) 270, 348
BslFI GGGAC 2 cut(s) 142, 549
BslI CCNNNNNNNGG 5 cut(s) 29, 581, 582, 583, 842
BsmAI GTCTC 1 cut(s) 613
BsmFI GGGAC 2 cut(s) 142, 549
BsnI GGCC 6 cut(s) 163, 225, 336, 521, 587, 928
Bsp119I TTCGAA 1 cut(s) 543
Bsp1286I GDGCHC 2 cut(s) 96, 894
Bsp143I GATC 1 cut(s) 712
Bsp19I CCATGG 1 cut(s) 894
BspANI GGCC 6 cut(s) 163, 225, 336, 521, 587, 928
BspCNI CTCAG 4 cut(s) 212, 543, 654, 754
BspLI GGNNCC 4 cut(s) 78, 268, 586, 622
BspMI ACCTGC 1 cut(s) 844
BspPI GGATC 1 cut(s) 707
BspT104I TTCGAA 1 cut(s) 543
BspT107I GGYRCC 2 cut(s) 266, 620
BsrDI GCAATG 1 cut(s) 606
BsrFI RCCGGY 1 cut(s) 269
BsrI ACTGG 1 cut(s) 118
BssAI RCCGGY 1 cut(s) 269
BssECI CCNNGG 6 cut(s) 60, 80, 204, 226, 337, 894
BssMI GATC 1 cut(s) 712
BssNI GRCGYC 1 cut(s) 621
BssSI CACGAG 1 cut(s) 450
BssT1I CCWWGG 3 cut(s) 80, 226, 894
Bst2BI CACGAG 1 cut(s) 450
Bst2UI CCWGG 4 cut(s) 24, 338, 589, 843
Bst4CI ACNGT 1 cut(s) 629
Bst6I CTCTTC 1 cut(s) 140
BstACI GRCGYC 1 cut(s) 621
BstBI TTCGAA 1 cut(s) 543
BstC8I GCNNGC 3 cut(s) 619, 819, 926
BstDEI CTNAG 4 cut(s) 199, 551, 662, 762
BstDSI CCRYGG 2 cut(s) 204, 894
BstENI CCTNNNNNAGG 2 cut(s) 27, 840
BstF5I GGATG 3 cut(s) 517, 569, 748
BstH2I RGCGCY 1 cut(s) 624
BstHHI GCGC 2 cut(s) 504, 623
BstKTI GATC 1 cut(s) 715
BstMAI GTCTC 1 cut(s) 613
BstMBI GATC 1 cut(s) 712
BstMWI GCNNNNNNNGC 9 cut(s) 53, 83, 231, 288, 321, 330, 339, 629, 814
BstNI CCWGG 4 cut(s) 24, 338, 589, 843
BstSCI CCNGG 5 cut(s) 22, 336, 346, 587, 841
BstV1I GCAGC 5 cut(s) 246, 365, 655, 881, 884
BsuRI GGCC 6 cut(s) 163, 225, 336, 521, 587, 928
BtgI CCRYGG 2 cut(s) 204, 894
BtsCI GGATG 3 cut(s) 517, 569, 748
BtsI GCAGTG 2 cut(s) 283, 289
BtsIMutI CAGTG 2 cut(s) 283, 289
BveI ACCTGC 1 cut(s) 844
Cac8I GCNNGC 3 cut(s) 619, 819, 926
CfoI GCGC 2 cut(s) 504, 623
Cfr10I RCCGGY 1 cut(s) 269
Cfr13I GGNCC 3 cut(s) 223, 585, 845
Csp6I GTAC 2 cut(s) 4, 67
CviAII CATG 2 cut(s) 440, 895
CviQI GTAC 2 cut(s) 4, 67
DdeI CTNAG 4 cut(s) 199, 551, 662, 762
DinI GGCGCC 1 cut(s) 622
DpnI GATC 1 cut(s) 714
DpnII GATC 1 cut(s) 712
EaeI YGGCCR 2 cut(s) 334, 926
Eam1104I CTCTTC 1 cut(s) 140
EarI CTCTTC 1 cut(s) 140
EciI GGCGGA 5 cut(s) 306, 759, 765, 771, 783
Eco130I CCWWGG 3 cut(s) 80, 226, 894
Eco147I AGGCCT 1 cut(s) 163
Eco24I GRGCYC 1 cut(s) 894
Eco47I GGWCC 1 cut(s) 845
EcoNI CCTNNNNNAGG 2 cut(s) 27, 840
EcoO109I RGGNCCY 1 cut(s) 585
EcoRI GAATTC 1 cut(s) 657
EcoRII CCWGG 4 cut(s) 22, 336, 587, 841
EcoT14I CCWWGG 3 cut(s) 80, 226, 894
EcoT38I GRGCYC 1 cut(s) 894
EgeI GGCGCC 1 cut(s) 622
EheI GGCGCC 1 cut(s) 622
ErhI CCWWGG 3 cut(s) 80, 226, 894
FaeI CATG 2 cut(s) 443, 898
FaiI YATR 8 cut(s) 360, 428, 441, 529, 531, 579, 896, 913
FaqI GGGAC 2 cut(s) 142, 549
FatI CATG 2 cut(s) 439, 894
FauI CCCGC 3 cut(s) 233, 317, 410
FauNDI CATATG 1 cut(s) 529
FokI GGATG 3 cut(s) 524, 576, 755
FriOI GRGCYC 1 cut(s) 894
FspBI CTAG 3 cut(s) 81, 489, 704
GlaI GCGC 2 cut(s) 503, 622
GsaI CCCAGC 1 cut(s) 405
GsuI CTGGAG 1 cut(s) 45
HaeII RGCGCY 1 cut(s) 624
HaeIII GGCC 6 cut(s) 163, 225, 336, 521, 587, 928
HapII CCGG 2 cut(s) 270, 348
HhaI GCGC 2 cut(s) 504, 623
Hin1I GRCGYC 1 cut(s) 621
Hin1II CATG 2 cut(s) 443, 898
Hin6I GCGC 2 cut(s) 502, 621
HinP1I GCGC 2 cut(s) 502, 621
HindIII AAGCTT 1 cut(s) 370
HinfI GANTC 1 cut(s) 900
HpaII CCGG 2 cut(s) 270, 348
HphI GGTGA 2 cut(s) 146, 722
Hpy188I TCNGA 3 cut(s) 64, 663, 717
Hpy188III TCNNGA 3 cut(s) 120, 550, 561
Hpy99I CGWCG 1 cut(s) 808
HpyAV CCTTC 6 cut(s) 23, 179, 214, 578, 717, 730
HpyCH4III ACNGT 1 cut(s) 629
HpyCH4IV ACGT 1 cut(s) 538
HpyCH4V TGCA 6 cut(s) 353, 473, 599, 632, 682, 839
HpyF10VI GCNNNNNNNGC 9 cut(s) 53, 83, 231, 288, 321, 330, 339, 629, 814
HpyF3I CTNAG 4 cut(s) 199, 551, 662, 762
HpySE526I ACGT 1 cut(s) 538
Hsp92I GRCGYC 1 cut(s) 621
Hsp92II CATG 2 cut(s) 443, 898
HspAI GCGC 2 cut(s) 502, 621
KasI GGCGCC 1 cut(s) 620
Kzo9I GATC 1 cut(s) 712
LmnI GCTCC 4 cut(s) 61, 82, 91, 420
Lsp1109I GCAGC 5 cut(s) 246, 365, 655, 881, 884
LweI GCATC 3 cut(s) 43, 554, 733
MaeI CTAG 3 cut(s) 81, 489, 704
MaeII ACGT 1 cut(s) 538
MalI GATC 1 cut(s) 714
MboI GATC 1 cut(s) 712
MboII GAAGA 5 cut(s) 26, 157, 184, 378, 745
MhlI GDGCHC 2 cut(s) 96, 894
MluCI AATT 2 cut(s) 633, 657
Mly113I GGCGCC 1 cut(s) 621
MseI TTAA 2 cut(s) 495, 651
MslI CAYNNNNRTG 1 cut(s) 677
MspA1I CMGCKG 1 cut(s) 237
MspI CCGG 2 cut(s) 270, 348
MspR9I CCNGG 5 cut(s) 24, 338, 348, 589, 843
MvaI CCWGG 4 cut(s) 24, 338, 589, 843
MwoI GCNNNNNNNGC 9 cut(s) 53, 83, 231, 288, 321, 330, 339, 629, 814
NarI GGCGCC 1 cut(s) 621
NciI CCSGG 1 cut(s) 348
NcoI CCATGG 1 cut(s) 894
NdeI CATATG 1 cut(s) 529
NdeII GATC 1 cut(s) 712
NlaIII CATG 2 cut(s) 443, 898
NlaIV GGNNCC 4 cut(s) 78, 268, 586, 622
NspV TTCGAA 1 cut(s) 543
OliI CACNNNNGTG 1 cut(s) 677
PaqCI CACCTGC 1 cut(s) 844
PceI AGGCCT 1 cut(s) 163
PfeI GAWTC 1 cut(s) 900
PluTI GGCGCC 1 cut(s) 624
PshBI ATTAAT 1 cut(s) 651
PsiI TTATAA 1 cut(s) 360
Psp6I CCWGG 4 cut(s) 22, 336, 587, 841
PspFI CCCAGC 1 cut(s) 401
PspGI CCWGG 4 cut(s) 22, 336, 587, 841
PspN4I GGNNCC 4 cut(s) 78, 268, 586, 622
PspPI GGNCC 3 cut(s) 223, 585, 845
RsaI GTAC 2 cut(s) 5, 68
RsaNI GTAC 2 cut(s) 4, 67
RseI CAYNNNNRTG 1 cut(s) 677
SaqAI TTAA 2 cut(s) 495, 651
Sau3AI GATC 1 cut(s) 712
Sau96I GGNCC 3 cut(s) 223, 585, 845
ScrFI CCNGG 5 cut(s) 24, 338, 348, 589, 843
SduI GDGCHC 2 cut(s) 96, 894
SfaNI GCATC 3 cut(s) 43, 554, 733
SfoI GGCGCC 1 cut(s) 622
SfuI TTCGAA 1 cut(s) 543
SinI GGWCC 1 cut(s) 845
SmiMI CAYNNNNRTG 1 cut(s) 677
Sse9I AATT 2 cut(s) 633, 657
SseBI AGGCCT 1 cut(s) 163
SspDI GGCGCC 1 cut(s) 620
SspMI CTAG 3 cut(s) 81, 489, 704
StuI AGGCCT 1 cut(s) 163
StyD4I CCNGG 5 cut(s) 22, 336, 346, 587, 841
StyI CCWWGG 3 cut(s) 80, 226, 894
TaaI ACNGT 1 cut(s) 629
TaiI ACGT 1 cut(s) 541
TaqI TCGA 1 cut(s) 543
TasI AATT 2 cut(s) 633, 657
TauI GCSGC 5 cut(s) 50, 110, 240, 333, 336
TfiI GAWTC 1 cut(s) 900
Tru1I TTAA 2 cut(s) 495, 651
Tru9I TTAA 2 cut(s) 495, 651
TscAI CASTG 2 cut(s) 283, 289
TseI GCWGC 5 cut(s) 234, 353, 668, 869, 872
TspDTI ATGAA 1 cut(s) 456
TspRI CASTG 2 cut(s) 283, 289
VpaK11BI GGWCC 1 cut(s) 845
VspI ATTAAT 1 cut(s) 651
XagI CCTNNNNNAGG 2 cut(s) 27, 840
XapI RAATTY 1 cut(s) 657
XmaJI CCTAGG 1 cut(s) 80
XspI CTAG 3 cut(s) 81, 489, 704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.