RchiOBHm_Chr4g0397451

U4 U6.U5 tri-snRNP-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002617
Physical Location & Seq
Forward (+)
13866477 .. 13867374
898 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 642 bp
ATGCAGCCGCCAAATTGGTGTAAGGAAAGGGATTGTCCATATCTGGATTCCGTTAACCGCCAGCTTGTGAGACAAAATTATCAAGGAAGAGGAAGAAACTCTCATGCTTATTCTCGTAGCCTCGAATTAGGGCACCATGTTTACATCAATCTCGTAATTGAAAAGTCCTATTGCCTTCCTGATGGATACGAAATCAACGATCCATCATTGGAAGACATTCGATATGTTCTAAACCCTAGGTTTAGTGAGAAACAAGTTAAGCAGATTGATAAAAACAAGCAGAGTTCTAGGGCAATGGACGGTTCTGATTACATTCCTGGAATGGTGGGGGTGAATAATTTTATGGAGAAGACTGATTTCGTGAATGCCACTGTTCAAAGTTTGATGAGGGTTGCGCCTCTACGAAACTTCCTCCTTGCTAAGAACTATGAAAGTTGCAAGTCTCGGCTTCTTCATCAATTCGGGGAGCTCACAAGGAAGATATGGAATGGGAGAAGCTTCAAAGGACCTGTGAGCCAGCATGACTTTCTGCAGGCGGTTATGGAAGCTAGTAAGAAGCGGCGTGGAGTGGGGTTGCAGTCTGATACTCTGATCCAATTGAGTTCATGTCATGGCTGCTCAATGCAATTCATGAGGATTTGA

Protein Analysis

213

Amino Acids

24.61

Weight (kDa)

9.33

Isoelectric Point (pI)

46.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-UBP PF02148 27 - 69 7.9e-06 Zn-finger in ubiquitin-hydrolases and other protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 132
AciI CCGC 4 cut(s) 8, 58, 536, 559
AclWI GGATC 2 cut(s) 194, 586
AgsI TTSAA 3 cut(s) 161, 377, 502
AjnI CCWGG 1 cut(s) 316
AluBI AGCT 4 cut(s) 64, 469, 498, 548
AluI AGCT 4 cut(s) 64, 469, 498, 548
Alw21I GWGCWC 1 cut(s) 471
Alw26I GTCTC 2 cut(s) 64, 447
AlwI GGATC 2 cut(s) 194, 586
ApeKI GCWGC 2 cut(s) 4, 615
Asp700I GAANNNNTTC 1 cut(s) 216
AspA2I CCTAGG 1 cut(s) 236
AspLEI GCGC 1 cut(s) 397
AspS9I GGNCC 1 cut(s) 506
AsuHPI GGTGA 1 cut(s) 343
AvaII GGWCC 1 cut(s) 506
AvrII CCTAGG 1 cut(s) 236
BaeGI GKGCMC 1 cut(s) 135
BanI GGYRCC 1 cut(s) 132
BanII GRGCYC 1 cut(s) 471
BbsI GAAGAC 2 cut(s) 219, 356
Bbv12I GWGCWC 1 cut(s) 471
BbvI GCAGC 2 cut(s) 16, 602
BccI CCATC 2 cut(s) 176, 211
BciT130I CCWGG 1 cut(s) 318
BciVI GTATCC 1 cut(s) 179
BcoDI GTCTC 2 cut(s) 64, 447
BfaI CTAG 3 cut(s) 237, 288, 549
BfmI CTRYAG 1 cut(s) 530
BfuI GTATCC 1 cut(s) 179
BisI GCNGC 4 cut(s) 5, 8, 560, 616
BlnI CCTAGG 1 cut(s) 236
BlsI GCNGC 4 cut(s) 6, 9, 561, 617
Bme1390I CCNGG 1 cut(s) 318
Bme18I GGWCC 1 cut(s) 506
BmgT120I GGNCC 1 cut(s) 506
BmiI GGNNCC 1 cut(s) 134
BmrFI CCNGG 1 cut(s) 318
BpiI GAAGAC 2 cut(s) 219, 356
BsaJI CCNNGG 1 cut(s) 236
Bse3DI GCAATG 1 cut(s) 300
BseBI CCWGG 1 cut(s) 318
BseDI CCNNGG 1 cut(s) 236
BseMI GCAATG 1 cut(s) 300
BseSI GKGCMC 1 cut(s) 135
BseXI GCAGC 2 cut(s) 16, 602
BshNI GGYRCC 1 cut(s) 132
BsiHKAI GWGCWC 1 cut(s) 471
BsmAI GTCTC 2 cut(s) 64, 447
BsmI GAATGC 1 cut(s) 370
Bsp1286I GDGCHC 2 cut(s) 135, 471
Bsp143I GATC 2 cut(s) 199, 591
BspACI CCGC 4 cut(s) 8, 58, 536, 559
BspHI TCATGA 1 cut(s) 630
BspLI GGNNCC 1 cut(s) 134
BspMAI CTGCAG 1 cut(s) 534
BspPI GGATC 2 cut(s) 194, 586
BspT107I GGYRCC 1 cut(s) 132
BsrDI GCAATG 1 cut(s) 300
BssECI CCNNGG 1 cut(s) 236
BssMI GATC 2 cut(s) 199, 591
BssT1I CCWWGG 1 cut(s) 236
Bst2UI CCWGG 1 cut(s) 318
Bst4CI ACNGT 2 cut(s) 302, 373
Bst6I CTCTTC 1 cut(s) 82
BstC8I GCNNGC 3 cut(s) 62, 518, 534
BstDEI CTNAG 1 cut(s) 420
BstHHI GCGC 1 cut(s) 397
BstKTI GATC 2 cut(s) 202, 594
BstMAI GTCTC 2 cut(s) 64, 447
BstMBI GATC 2 cut(s) 199, 591
BstNI CCWGG 1 cut(s) 318
BstSCI CCNGG 1 cut(s) 316
BstSFI CTRYAG 1 cut(s) 530
BstSLI GKGCMC 1 cut(s) 135
BstV1I GCAGC 2 cut(s) 16, 602
BstV2I GAAGAC 2 cut(s) 219, 356
BsuI GTATCC 1 cut(s) 179
BtsIMutI CAGTG 1 cut(s) 369
Cac8I GCNNGC 3 cut(s) 62, 518, 534
CciI TCATGA 1 cut(s) 630
CfoI GCGC 1 cut(s) 397
Cfr13I GGNCC 1 cut(s) 506
CviAII CATG 6 cut(s) 104, 137, 521, 606, 611, 631
CviJI RGCY 9 cut(s) 7, 64, 120, 448, 469, 498, 516, 548, 615
CviKI_1 RGCY 9 cut(s) 7, 64, 120, 448, 469, 498, 516, 548, 615
DdeI CTNAG 1 cut(s) 420
DpnI GATC 2 cut(s) 201, 593
DpnII GATC 2 cut(s) 199, 591
Eam1104I CTCTTC 1 cut(s) 82
EarI CTCTTC 1 cut(s) 82
Ecl136II GAGCTC 1 cut(s) 469
Eco130I CCWWGG 1 cut(s) 236
Eco24I GRGCYC 1 cut(s) 471
Eco47I GGWCC 1 cut(s) 506
Eco53kI GAGCTC 1 cut(s) 469
EcoICRI GAGCTC 1 cut(s) 469
EcoO109I RGGNCCY 1 cut(s) 506
EcoRII CCWGG 1 cut(s) 316
EcoT14I CCWWGG 1 cut(s) 236
EcoT38I GRGCYC 1 cut(s) 471
ErhI CCWWGG 1 cut(s) 236
FaeI CATG 6 cut(s) 107, 140, 524, 609, 614, 634
FatI CATG 6 cut(s) 103, 136, 520, 605, 610, 630
Fnu4HI GCNGC 4 cut(s) 5, 8, 560, 616
FriOI GRGCYC 1 cut(s) 471
Fsp4HI GCNGC 4 cut(s) 5, 8, 560, 616
FspBI CTAG 3 cut(s) 237, 288, 549
GlaI GCGC 1 cut(s) 396
GluI GCNGC 4 cut(s) 5, 8, 560, 616
HhaI GCGC 1 cut(s) 397
Hin1II CATG 6 cut(s) 107, 140, 524, 609, 614, 634
Hin6I GCGC 1 cut(s) 395
HinP1I GCGC 1 cut(s) 395
HincII GTYRAC 1 cut(s) 55
HindII GTYRAC 1 cut(s) 55
HindIII AAGCTT 1 cut(s) 496
HinfI GANTC 1 cut(s) 47
HpaI GTTAAC 1 cut(s) 55
HphI GGTGA 1 cut(s) 343
Hpy166II GTNNAC 2 cut(s) 55, 142
Hpy188I TCNGA 3 cut(s) 307, 583, 591
Hpy188III TCNNGA 4 cut(s) 44, 179, 361, 631
Hpy8I GTNNAC 2 cut(s) 55, 142
HpyAV CCTTC 1 cut(s) 185
HpyCH4III ACNGT 2 cut(s) 302, 373
HpyCH4V TGCA 5 cut(s) 4, 438, 532, 577, 625
HpyF3I CTNAG 1 cut(s) 420
Hsp92II CATG 6 cut(s) 107, 140, 524, 609, 614, 634
HspAI GCGC 1 cut(s) 395
KspAI GTTAAC 1 cut(s) 55
Kzo9I GATC 2 cut(s) 199, 591
LmnI GCTCC 1 cut(s) 466
LpnPI CCDG 8 cut(s) 29, 74, 192, 303, 330, 518, 522, 530
Lsp1109I GCAGC 2 cut(s) 16, 602
MaeI CTAG 3 cut(s) 237, 288, 549
MalI GATC 2 cut(s) 201, 593
MboI GATC 2 cut(s) 199, 591
MboII GAAGA 6 cut(s) 99, 105, 224, 361, 443, 490
MfeI CAATTG 1 cut(s) 596
MhlI GDGCHC 2 cut(s) 135, 471
MluCI AATT 8 cut(s) 13, 76, 125, 156, 337, 458, 596, 626
MnlI CCTC 6 cut(s) 83, 131, 381, 408, 422, 627
MroXI GAANNNNTTC 1 cut(s) 216
MseI TTAA 2 cut(s) 54, 258
MspR9I CCNGG 1 cut(s) 318
MunI CAATTG 1 cut(s) 596
Mva1269I GAATGC 1 cut(s) 370
MvaI CCWGG 1 cut(s) 318
NdeII GATC 2 cut(s) 199, 591
NlaIII CATG 6 cut(s) 107, 140, 524, 609, 614, 634
NlaIV GGNNCC 1 cut(s) 134
NmeAIII GCCGAG 1 cut(s) 424
PagI TCATGA 1 cut(s) 630
PcsI WCGNNNNNNNCGW 1 cut(s) 195
PctI GAATGC 1 cut(s) 370
PdmI GAANNNNTTC 1 cut(s) 216
PfeI GAWTC 1 cut(s) 47
PfoI TCCNGGA 1 cut(s) 316
PkrI GCNGC 4 cut(s) 6, 9, 561, 617
PpuMI RGGWCCY 1 cut(s) 506
Psp124BI GAGCTC 1 cut(s) 471
Psp5II RGGWCCY 1 cut(s) 506
Psp6I CCWGG 1 cut(s) 316
PspGI CCWGG 1 cut(s) 316
PspN4I GGNNCC 1 cut(s) 134
PspPI GGNCC 1 cut(s) 506
PspPPI RGGWCCY 1 cut(s) 506
PstI CTGCAG 1 cut(s) 534
SacI GAGCTC 1 cut(s) 471
SaqAI TTAA 2 cut(s) 54, 258
SatI GCNGC 4 cut(s) 5, 8, 560, 616
Sau3AI GATC 2 cut(s) 199, 591
Sau96I GGNCC 1 cut(s) 506
ScrFI CCNGG 1 cut(s) 318
SduI GDGCHC 2 cut(s) 135, 471
SetI ASST 6 cut(s) 66, 242, 471, 500, 511, 550
SfcI CTRYAG 1 cut(s) 530
SinI GGWCC 1 cut(s) 506
Sse9I AATT 8 cut(s) 13, 76, 125, 156, 337, 458, 596, 626
SsiI CCGC 4 cut(s) 8, 58, 536, 559
SspMI CTAG 3 cut(s) 237, 288, 549
SstI GAGCTC 1 cut(s) 471
StyD4I CCNGG 1 cut(s) 316
StyI CCWWGG 1 cut(s) 236
TaaI ACNGT 2 cut(s) 302, 373
TaqI TCGA 2 cut(s) 123, 220
TasI AATT 8 cut(s) 13, 76, 125, 156, 337, 458, 596, 626
TauI GCSGC 2 cut(s) 10, 562
TfiI GAWTC 1 cut(s) 47
Tru1I TTAA 2 cut(s) 54, 258
Tru9I TTAA 2 cut(s) 54, 258
TscAI CASTG 1 cut(s) 376
TseI GCWGC 2 cut(s) 4, 615
TspDTI ATGAA 4 cut(s) 443, 444, 594, 619
TspGWI ACGGA 1 cut(s) 40
TspRI CASTG 1 cut(s) 376
VpaK11BI GGWCC 1 cut(s) 506
XmaJI CCTAGG 1 cut(s) 236
XmnI GAANNNNTTC 1 cut(s) 216
XspI CTAG 3 cut(s) 237, 288, 549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.