RchiOBHm_Chr4g0406851

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0003224
Physical Location & Seq
Forward (+)
28774744 .. 28787545
12802 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2967 bp
ATGGGGGTTGTATCGAGGCGAGTTGTGCCCGCCTGCGGTAGCCTCTGTTTCTTCTGCCCCTCACTGCGTGCAAGGTCGAGGCAACCAGTCAAACGCTACAAGAAGTTGCTCGCTGAGATCTTCCCCCGTAATCAGGATGCTGAACCCAATGACAGAAAGATTGGGAAACTTTGTGAATATGCTCTGAAGAACCCATTGCGAATTCCCAAGATTACTGAGCATCTAGAGCAAAAATGTTACAAGGATTTGCGAAACGAACAGTTTGGATCTGTTAAAGTCATACTGTGCATTTATAGGAAGTTACTATCTTCCTGTAAACTGCAGATGCCACTATTTGCAAGTAGTCTACTAGGAATTGTTCGGATTCTCTTAGAACAAACCCGACATGATGAAATGCGGATTCTGGGTTGCAAAACACTTGTCGACTTTATAAATAGCCAGATTGATAGCACACACATGTTCAACCTAGAAGGCCTTATTCCTAAACTCTGTGAATTGGCTCAAGAAATGGGAGATGATGAAAGAGCTCTACGTCTACGTTCAGCTGGATTGCAATCACTGGCTTTTATGGTGTGGTTCATGGGTGAGCACTCTCATATCTCGATGGATTTTGACAATATCATATCAGTGACTTTGGAAAATTACACGGATATGCATACTAAATCTGGGAGTGTGAAAGAAGACGGACAATTTTCTGAATCTCAGCACCAGTGGGTTCAAGAAGCGCTAAAAGCAGAAGTTCATGATTCATCTTTTCCAGATGTTAGTCAGAAGGTTCTTTCATTACCAAACCTGAAAACCCCTGATTTGGACCCCACAATTGATACAGACAAGAGTCCGTCTTACTGGTCAAAGGTTTGCTTGCGTAATATAGCCAGATTGGCAAAGGAAGCTACAACAGTGCGTCGAGTGCTTGAACCCCTTTTCCAAAATTTTGATGCTGCGAACCATTGGTCCCCAGAAAAACCACTTGCCTATTATGTTTTAATGTATCTGCAGTCACTATTAGAGGAATCAGGAGATAACTCTCATCTGTTATTATCCATATTGGTCAAGCACTTGGATCATAAGAATGTTGTCAAACAGCCTCGCTTGCAAACTGATATTGTAAATGTCACCACACAAATAGCACAAAGTGCAAAGCAGCAGGCATCAGTTGCGATTATTGGTGCAATATCTGACCTGACAAAGCATTTACGAAAATGCCTGCAAAATCAAGCTGAAGGGTCCAACCGTAGTAGTACAGATAAATGGAACCAAGATCTTCAGTCAGCACTAGAGAGATGCATCTTGCAACTGTCAAATAAGGTTGGAGATGTGGGGCCCATACTTGATATGATGGCTGTGGTGCTGGAGAATATCCCATCTAGTACTATTGTAGCTAGGGCGACAGTCTCTGCTGTTTATCTAACTGCAAAGATGGTCTCTTCTGTTCCAAACATATCCTATCACAAAAAGCCATTCCCTGATGCTCTGTTTCACCAGTTACTCCTAGCAATGGCCCACCTAGACCATGAAACACGTGTTGGAGCACACAGTATTTTCTCTATTGTTCTTGTGCCATCTCTACTTGTTCCTTGGCTACAGCAGAAAATGAACTCTGTGCAGACTGTTTCTGGGTTTTCATCTGTCAGTAGATTGGAGTTTGGAAAGGATAAAAGAAAAGACACGGGAGCAACCGCGAATGGAGAGTTGAGGGAAGAGGAAAGTCAAATATCAGATGTTTGTGAAAATCAATCTGGCAGCTTCAAGAGTGCTTTGACGGGTGGAAGAGCAGAACTACCTTGCCTTCGGTTAAGCAGTCATCAAGTGAGTCTATTGCTTTCATTAATCTGGGTTCAGGCAACATCTGCAGAGAATACCCCAGCAAATTTTGAGGCGATGGCTCATTCTTATAATGTTGCTTTACTGTTTACCCGATCAAAGGCTTCAAGTCATATGGCTTTAGTAAGATGCTTCCAGTTGGCATTCTCCATCAGGACTATTTCCTTGGATCGAGATGGGGGTTTGCAAGAATCTCGTAGAAGGTCTCTTTATACGTTGGCATCATACATGCTTATATTTGCAGCCCGGGCGGGGAACTTCCCGGAGCTAATTCCCATTGTTAAAGCATCATTGATGGATCAAATGGTTGATCCTTGTCTTCAGTTGGTTGACGATATCCGACTGCAGGCAGTTTCAATTGACTCCGATATGGAGAAGATACCCTATGGATCACAGCCAGACGAAGTTGCTGCATTGAAGTCTCATTCAGCAATTGAACTAGATGACCAATTGTTGAAAGAAACCGTGATATCCCACTTCATGACTAAATTTTCCATATTGTCAGAGGATGAATTGTCTAGCATCAAGAAGCAACTCTTACTGGGTTTTTCACCGGATGATGCATTTCCATTAGGAGCTCCACTATTCATGGAGACACCACGACCATGTTCTCCACTTGCCCATATAGAATTTGCTGATTTTGATGAGGTGATGCCTCCAGGTTCTTTGACAGATGAAGAAGCCTTCCTTGAACCTAGTGGAAGTCAGTCAGACCGCAAGACATCACTTTCCATCAATGCACTTGACATTTTAAATGTTAATCAGCTCCTGGATTCAGTTCTAGAAACGGCCCAACAAGTTGCAAGCTTCCCAGTCTCCACAATGCCTGTACCTTATGACCAAATGAAGAGCCAATGTGAAGCCCTTGTAACGGGCAAACAGCAAAAGATGGCAGTTCTTCATAGTTTCAAGCATCAACTAGAAACCAAGGCGTTAGTGCTCTCCAATGTAAATGAAAACAAATCCCATGCTTCACCACCCATGGGATTGGAATCTTCAGAAGGGTACCCAAAGGTCAAAACCAAGGAGCAAATTCAAGCGAAAAACCAGCTCGTCATATGTTCTCGTGAGTATGGACAGCATTCTTTTAAATTACCACCTTCAAGTCCTTATGACAAATTCTTGAAAGCTGCTGGATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

988

Amino Acids

110.18

Weight (kDa)

6.49

Isoelectric Point (pI)

49.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EFR3_ARM PF21052 65 - 151 1e-17 EFR3, armadillo repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 431, 1896
Acc65I GGTACC 1 cut(s) 2829
AccB1I GGYRCC 1 cut(s) 2829
AccI GTMKAC 3 cut(s) 346, 423, 535
AccII CGCG 1 cut(s) 1682
AciI CCGC 6 cut(s) 30, 36, 397, 1680, 2075, 2539
AclWI GGATC 6 cut(s) 274, 1071, 2001, 2129, 2130, 2221
AcsI RAATTY 7 cut(s) 201, 931, 1870, 2312, 2453, 2856, 2942
AcuI CTGAAG 5 cut(s) 206, 1242, 1250, 2129, 2805
AcvI CACGTG 1 cut(s) 1523
AdeI CACNNNGTG 2 cut(s) 68, 1136
AfaI GTAC 4 cut(s) 1243, 1372, 2655, 2831
AfeI AGCGCT 1 cut(s) 726
AfiI CCNNNNNNNGG 9 cut(s) 35, 133, 808, 1498, 1924, 2076, 2170, 2695, 2807
AflIII ACRYGT 3 cut(s) 456, 1520, 1522
AjnI CCWGG 2 cut(s) 2482, 2592
AjuI GAANNNNNNNTTGG 2 cut(s) 1509, 1541
AloI GAACNNNNNNTCC 4 cut(s) 909, 938, 941, 970
Alw21I GWGCWC 5 cut(s) 529, 591, 1534, 2404, 2766
Alw26I GTCTC 6 cut(s) 1399, 1429, 2034, 2250, 2411, 2644
AlwI GGATC 6 cut(s) 274, 1071, 2001, 2129, 2130, 2221
AlwNI CAGNNNCTG 2 cut(s) 1397, 2593
Ama87I CYCGRG 1 cut(s) 2070
Aor51HI AGCGCT 1 cut(s) 726
AoxI GGCC 4 cut(s) 472, 1322, 1500, 2613
ApaI GGGCCC 1 cut(s) 1326
ApeKI GCWGC 6 cut(s) 941, 1144, 1743, 2066, 2234, 2954
ApoI RAATTY 7 cut(s) 201, 931, 1870, 2312, 2453, 2856, 2942
ArsI GACNNNNNNTTYG 4 cut(s) 405, 437, 938, 970
AseI ATTAAT 1 cut(s) 1829
Asp718I GGTACC 1 cut(s) 2829
AspLEI GCGC 1 cut(s) 727
AspS9I GGNCC 7 cut(s) 811, 954, 1227, 1322, 1323, 1501, 2614
AsuC2I CCSGG 3 cut(s) 2071, 2072, 2087
AsuHPI GGTGA 6 cut(s) 596, 1108, 1472, 2367, 2485, 2790
AvaI CYCGRG 1 cut(s) 2070
AvaII GGWCC 3 cut(s) 811, 954, 1227
BaeGI GKGCMC 2 cut(s) 30, 1326
BanI GGYRCC 1 cut(s) 2829
BanII GRGCYC 3 cut(s) 529, 1326, 2404
BauI CACGAG 1 cut(s) 2889
BbrPI CACGTG 1 cut(s) 1523
BbsI GAAGAC 2 cut(s) 687, 2135
Bbv12I GWGCWC 5 cut(s) 529, 591, 1534, 2404, 2766
BbvI GCAGC 6 cut(s) 928, 1156, 1755, 2078, 2221, 2941
BceAI ACGGC 1 cut(s) 2628
BcgI CGANNNNNNTGC 4 cut(s) 2000, 2034, 2216, 2250
BciT130I CCWGG 2 cut(s) 2484, 2594
BcnI CCSGG 3 cut(s) 2071, 2072, 2087
BcoDI GTCTC 6 cut(s) 1399, 1429, 2034, 2250, 2411, 2644
BfmI CTRYAG 5 cut(s) 320, 995, 1583, 1851, 2168
BfoI RGCGCY 1 cut(s) 728
BglI GCCNNNNNGGC 1 cut(s) 881
BglII AGATCT 2 cut(s) 117, 1261
BisI GCNGC 6 cut(s) 942, 1145, 1744, 2067, 2235, 2955
BlsI GCNGC 6 cut(s) 943, 1146, 1745, 2068, 2236, 2956
BmcAI AGTACT 1 cut(s) 1372
Bme1390I CCNGG 5 cut(s) 2071, 2072, 2087, 2484, 2594
Bme18I GGWCC 3 cut(s) 811, 954, 1227
BmeT110I CYCGRG 1 cut(s) 2070
BmgT120I GGNCC 7 cut(s) 811, 954, 1227, 1322, 1323, 1501, 2614
BmiI GGNNCC 7 cut(s) 813, 956, 1228, 1256, 1323, 1324, 2831
BmrFI CCNGG 5 cut(s) 2071, 2072, 2087, 2484, 2594
BmrI ACTGGG 2 cut(s) 2375, 2630
BmuI ACTGGG 2 cut(s) 2375, 2630
BoxI GACNNNNGTC 1 cut(s) 834
BpiI GAAGAC 2 cut(s) 687, 2135
BpmI CTGGAG 2 cut(s) 1373, 2466
BpuEI CTTGAG 1 cut(s) 486
BpuMI CCSGG 3 cut(s) 2071, 2072, 2087
BsaAI YACGTR 1 cut(s) 1523
BsaBI GATNNNNATC 2 cut(s) 553, 2815
BsaI GGTCTC 2 cut(s) 1429, 2034
BsaJI CCNNGG 6 cut(s) 1577, 1989, 2070, 2751, 2805, 2847
BsaWI WCCGGW 1 cut(s) 2377
Bsc4I CCNNNNNNNGG 9 cut(s) 35, 133, 808, 1498, 1924, 2076, 2170, 2695, 2807
Bse1I ACTGG 8 cut(s) 86, 564, 709, 851, 1483, 1960, 2370, 2636
Bse3DI GCAATG 2 cut(s) 194, 1503
Bse8I GATNNNNATC 2 cut(s) 553, 2815
BseBI CCWGG 2 cut(s) 2484, 2594
BseDI CCNNGG 6 cut(s) 1577, 1989, 2070, 2751, 2805, 2847
BseGI GGATG 4 cut(s) 142, 2338, 2386, 2966
BseJI GATNNNNATC 2 cut(s) 553, 2815
BseLI CCNNNNNNNGG 9 cut(s) 35, 133, 808, 1498, 1924, 2076, 2170, 2695, 2807
BseMI GCAATG 2 cut(s) 194, 1503
BseMII CTCAG 3 cut(s) 105, 207, 716
BseNI ACTGG 8 cut(s) 86, 564, 709, 851, 1483, 1960, 2370, 2636
BseSI GKGCMC 2 cut(s) 30, 1326
BseXI GCAGC 6 cut(s) 928, 1156, 1755, 2078, 2221, 2941
BseYI CCCAGC 1 cut(s) 1864
BsgI GTGCAG 1 cut(s) 1625
Bsh1236I CGCG 1 cut(s) 1682
BshFI GGCC 4 cut(s) 474, 1324, 1502, 2615
BshNI GGYRCC 1 cut(s) 2829
BsiHKAI GWGCWC 5 cut(s) 529, 591, 1534, 2404, 2766
BsiHKCI CYCGRG 1 cut(s) 2070
BsiSI CCGG 3 cut(s) 2071, 2087, 2378
BslFI GGGAC 1 cut(s) 940
BslI CCNNNNNNNGG 9 cut(s) 35, 133, 808, 1498, 1924, 2076, 2170, 2695, 2807
BsmAI GTCTC 6 cut(s) 1399, 1429, 2034, 2250, 2411, 2644
BsmFI GGGAC 1 cut(s) 940
BsmI GAATGC 2 cut(s) 1967, 2905
BsnI GGCC 4 cut(s) 474, 1324, 1502, 2615
Bso31I GGTCTC 2 cut(s) 1429, 2034
BsoBI CYCGRG 1 cut(s) 2070
Bsp120I GGGCCC 1 cut(s) 1322
Bsp1286I GDGCHC 7 cut(s) 30, 529, 591, 1326, 1534, 2404, 2766
Bsp143I GATC 9 cut(s) 117, 266, 1063, 1261, 1919, 1993, 2122, 2134, 2213
Bsp19I CCATGG 1 cut(s) 2805
BspACI CCGC 6 cut(s) 30, 36, 397, 1680, 2075, 2539
BspANI GGCC 4 cut(s) 474, 1324, 1502, 2615
BspCNI CTCAG 3 cut(s) 106, 208, 715
BspFNI CGCG 1 cut(s) 1682
BspHI TCATGA 2 cut(s) 742, 2304
BspLI GGNNCC 7 cut(s) 813, 956, 1228, 1256, 1323, 1324, 2831
BspMAI CTGCAG 4 cut(s) 324, 999, 1855, 2172
BspPI GGATC 6 cut(s) 274, 1071, 2001, 2129, 2130, 2221
BspQI GCTCTTC 2 cut(s) 1765, 2666
BspT107I GGYRCC 1 cut(s) 2829
BspTNI GGTCTC 2 cut(s) 1429, 2034
BsrDI GCAATG 2 cut(s) 194, 1503
BsrI ACTGG 8 cut(s) 86, 564, 709, 851, 1483, 1960, 2370, 2636
BssECI CCNNGG 6 cut(s) 1577, 1989, 2070, 2751, 2805, 2847
BssMI GATC 9 cut(s) 117, 266, 1063, 1261, 1919, 1993, 2122, 2134, 2213
BssSI CACGAG 1 cut(s) 2889
BssT1I CCWWGG 5 cut(s) 1577, 1989, 2751, 2805, 2847
Bst2BI CACGAG 1 cut(s) 2889
Bst2UI CCWGG 2 cut(s) 2484, 2594
Bst6I CTCTTC 4 cut(s) 1432, 1695, 1765, 2666
BstAPI GCANNNNNTGC 3 cut(s) 1136, 1157, 1850
BstBAI YACGTR 1 cut(s) 1523
BstDEI CTNAG 4 cut(s) 114, 216, 370, 702
BstDSI CCRYGG 1 cut(s) 2805
BstF5I GGATG 4 cut(s) 142, 2338, 2386, 2966
BstFNI CGCG 1 cut(s) 1682
BstH2I RGCGCY 1 cut(s) 728
BstHHI GCGC 1 cut(s) 727
BstKTI GATC 9 cut(s) 120, 269, 1066, 1264, 1922, 1996, 2125, 2137, 2216
BstMAI GTCTC 6 cut(s) 1399, 1429, 2034, 2250, 2411, 2644
BstMBI GATC 9 cut(s) 117, 266, 1063, 1261, 1919, 1993, 2122, 2134, 2213
BstNI CCWGG 2 cut(s) 2484, 2594
BstNSI RCATGY 2 cut(s) 460, 2056
BstPAI GACNNNNGTC 1 cut(s) 834
BstSCI CCNGG 5 cut(s) 2069, 2070, 2085, 2482, 2592
BstSFI CTRYAG 5 cut(s) 320, 995, 1583, 1851, 2168
BstSLI GKGCMC 2 cut(s) 30, 1326
BstUI CGCG 1 cut(s) 1682
BstV1I GCAGC 6 cut(s) 928, 1156, 1755, 2078, 2221, 2941
BstV2I GAAGAC 2 cut(s) 687, 2135
BstX2I RGATCY 3 cut(s) 117, 266, 1261
BstXI CCANNNNNNTGG 1 cut(s) 2812
BstYI RGATCY 3 cut(s) 117, 266, 1261
BsuRI GGCC 4 cut(s) 474, 1324, 1502, 2615
BtgI CCRYGG 1 cut(s) 2805
BtgZI GCGATG 1 cut(s) 1895
BtsCI GGATG 4 cut(s) 142, 2338, 2386, 2966
BtsI GCAGTG 1 cut(s) 62
BtsIMutI CAGTG 5 cut(s) 62, 557, 633, 716, 906
CaiI CAGNNNCTG 2 cut(s) 1397, 2593
CciI TCATGA 2 cut(s) 742, 2304
CfoI GCGC 1 cut(s) 727
Cfr13I GGNCC 7 cut(s) 811, 954, 1227, 1322, 1323, 1501, 2614
Cfr9I CCCGGG 1 cut(s) 2070
CseI GACGC 1 cut(s) 893
Csp6I GTAC 4 cut(s) 1242, 1371, 2654, 2830
CviQI GTAC 4 cut(s) 1242, 1371, 2654, 2830
DdeI CTNAG 4 cut(s) 114, 216, 370, 702
DpnI GATC 9 cut(s) 119, 268, 1065, 1263, 1921, 1995, 2124, 2136, 2215
DpnII GATC 9 cut(s) 117, 266, 1063, 1261, 1919, 1993, 2122, 2134, 2213
DraI TTTAAA 2 cut(s) 2577, 2914
DraIII CACNNNGTG 2 cut(s) 68, 1136
Eam1104I CTCTTC 4 cut(s) 1432, 1695, 1765, 2666
EarI CTCTTC 4 cut(s) 1432, 1695, 1765, 2666
Ecl136II GAGCTC 2 cut(s) 527, 2402
Eco130I CCWWGG 5 cut(s) 1577, 1989, 2751, 2805, 2847
Eco147I AGGCCT 1 cut(s) 474
Eco24I GRGCYC 3 cut(s) 529, 1326, 2404
Eco31I GGTCTC 2 cut(s) 1429, 2034
Eco32I GATATC 2 cut(s) 2161, 2295
Eco47I GGWCC 3 cut(s) 811, 954, 1227
Eco47III AGCGCT 1 cut(s) 726
Eco53kI GAGCTC 2 cut(s) 527, 2402
Eco57I CTGAAG 5 cut(s) 206, 1242, 1250, 2129, 2805
Eco72I CACGTG 1 cut(s) 1523
Eco88I CYCGRG 1 cut(s) 2070
EcoICRI GAGCTC 2 cut(s) 527, 2402
EcoO109I RGGNCCY 1 cut(s) 1322
EcoRI GAATTC 1 cut(s) 201
EcoRII CCWGG 2 cut(s) 2482, 2592
EcoRV GATATC 2 cut(s) 2161, 2295
EcoT14I CCWWGG 5 cut(s) 1577, 1989, 2751, 2805, 2847
EcoT22I ATGCAT 3 cut(s) 657, 1289, 2389
EcoT38I GRGCYC 3 cut(s) 529, 1326, 2404
ErhI CCWWGG 5 cut(s) 1577, 1989, 2751, 2805, 2847
FalI AAGNNNNNCTT 6 cut(s) 845, 877, 2345, 2377, 2496, 2528
FaqI GGGAC 1 cut(s) 940
FauI CCCGC 2 cut(s) 37, 2068
FauNDI CATATG 2 cut(s) 1938, 2882
FblI GTMKAC 3 cut(s) 346, 423, 535
Fnu4HI GCNGC 6 cut(s) 942, 1145, 1744, 2067, 2235, 2955
FokI GGATG 3 cut(s) 149, 2345, 2393
FriOI GRGCYC 3 cut(s) 529, 1326, 2404
Fsp4HI GCNGC 6 cut(s) 942, 1145, 1744, 2067, 2235, 2955
GlaI GCGC 1 cut(s) 726
GluI GCNGC 6 cut(s) 942, 1145, 1744, 2067, 2235, 2955
GsaI CCCAGC 1 cut(s) 1868
GsuI CTGGAG 2 cut(s) 1373, 2466
HaeII RGCGCY 1 cut(s) 728
HaeIII GGCC 4 cut(s) 474, 1324, 1502, 2615
HapII CCGG 3 cut(s) 2071, 2087, 2378
HgaI GACGC 1 cut(s) 893
HhaI GCGC 1 cut(s) 727
Hin6I GCGC 1 cut(s) 725
HinP1I GCGC 1 cut(s) 725
HincII GTYRAC 2 cut(s) 424, 2155
HindII GTYRAC 2 cut(s) 424, 2155
HindIII AAGCTT 1 cut(s) 2629
HpaII CCGG 3 cut(s) 2071, 2087, 2378
HphI GGTGA 6 cut(s) 596, 1108, 1472, 2367, 2485, 2790
Hpy166II GTNNAC 6 cut(s) 317, 347, 424, 536, 1914, 2155
Hpy8I GTNNAC 6 cut(s) 317, 347, 424, 536, 1914, 2155
Hpy99I CGWCG 1 cut(s) 909
HpyAV CCTTC 8 cut(s) 464, 766, 1217, 1799, 2019, 2518, 2819, 2934
HpyCH4IV ACGT 4 cut(s) 532, 538, 1522, 2039
HpyF3I CTNAG 4 cut(s) 114, 216, 370, 702
HpySE526I ACGT 4 cut(s) 532, 538, 1522, 2039
HspAI GCGC 1 cut(s) 725
KpnI GGTACC 1 cut(s) 2833
Kzo9I GATC 9 cut(s) 117, 266, 1063, 1261, 1919, 1993, 2122, 2134, 2213
LguI GCTCTTC 2 cut(s) 1765, 2666
LmnI GCTCC 7 cut(s) 1529, 1673, 2089, 2399, 2407, 2595, 2851
Lsp1109I GCAGC 6 cut(s) 928, 1156, 1755, 2078, 2221, 2941
MaeII ACGT 4 cut(s) 532, 538, 1522, 2039
MaeIII GTNAC 7 cut(s) 236, 300, 628, 999, 1114, 1485, 2692
MalI GATC 9 cut(s) 119, 268, 1065, 1263, 1921, 1995, 2124, 2136, 2215
MboI GATC 9 cut(s) 117, 266, 1063, 1261, 1919, 1993, 2122, 2134, 2213
MfeI CAATTG 4 cut(s) 819, 2181, 2256, 2273
MflI RGATCY 3 cut(s) 117, 266, 1261
MhlI GDGCHC 7 cut(s) 30, 529, 591, 1326, 1534, 2404, 2766
MlyI GAGTC 3 cut(s) 844, 1822, 2180
MmeI TCCRAC 4 cut(s) 1254, 1291, 1507, 2188
Mph1103I ATGCAT 3 cut(s) 657, 1289, 2389
MseI TTAA 9 cut(s) 273, 986, 1796, 1829, 2106, 2576, 2583, 2913, 2965
MslI CAYNNNNRTG 5 cut(s) 455, 626, 650, 1071, 2428
MspA1I CMGCKG 1 cut(s) 545
MspI CCGG 3 cut(s) 2071, 2087, 2378
MspR9I CCNGG 5 cut(s) 2071, 2072, 2087, 2484, 2594
MunI CAATTG 4 cut(s) 819, 2181, 2256, 2273
Mva1269I GAATGC 2 cut(s) 1967, 2905
MvaI CCWGG 2 cut(s) 2484, 2594
MvnI CGCG 1 cut(s) 1682
NciI CCSGG 3 cut(s) 2071, 2072, 2087
NcoI CCATGG 1 cut(s) 2805
NdeI CATATG 2 cut(s) 1938, 2882
NdeII GATC 9 cut(s) 117, 266, 1063, 1261, 1919, 1993, 2122, 2134, 2213
NlaIV GGNNCC 7 cut(s) 813, 956, 1228, 1256, 1323, 1324, 2831
NmuCI GTSAC 3 cut(s) 628, 999, 1114
NsiI ATGCAT 3 cut(s) 657, 1289, 2389
NspI RCATGY 2 cut(s) 460, 2056
PagI TCATGA 2 cut(s) 742, 2304
PceI AGGCCT 1 cut(s) 474
PciI ACATGT 1 cut(s) 456
PciSI GCTCTTC 2 cut(s) 1765, 2666
PctI GAATGC 2 cut(s) 1967, 2905
PfeI GAWTC 8 cut(s) 364, 400, 698, 746, 1013, 2015, 2597, 2816
PfoI TCCNGGA 2 cut(s) 2085, 2592
PkrI GCNGC 6 cut(s) 943, 1146, 1745, 2068, 2236, 2956
PleI GAGTC 3 cut(s) 843, 1821, 2180
PmaCI CACGTG 1 cut(s) 1523
PmlI CACGTG 1 cut(s) 1523
PpsI GAGTC 3 cut(s) 843, 1821, 2180
Ppu21I YACGTR 1 cut(s) 1523
PscI ACATGT 1 cut(s) 456
PshAI GACNNNNGTC 1 cut(s) 834
PshBI ATTAAT 1 cut(s) 1829
PsiI TTATAA 2 cut(s) 431, 1896
Psp124BI GAGCTC 2 cut(s) 529, 2404
Psp6I CCWGG 2 cut(s) 2482, 2592
PspCI CACGTG 1 cut(s) 1523
PspFI CCCAGC 1 cut(s) 1864
PspGI CCWGG 2 cut(s) 2482, 2592
PspN4I GGNNCC 7 cut(s) 813, 956, 1228, 1256, 1323, 1324, 2831
PspOMI GGGCCC 1 cut(s) 1322
PspPI GGNCC 7 cut(s) 811, 954, 1227, 1322, 1323, 1501, 2614
PstI CTGCAG 4 cut(s) 324, 999, 1855, 2172
PstNI CAGNNNCTG 2 cut(s) 1397, 2593
PsuI RGATCY 3 cut(s) 117, 266, 1261
PvuII CAGCTG 1 cut(s) 545
RsaI GTAC 4 cut(s) 1243, 1372, 2655, 2831
RsaNI GTAC 4 cut(s) 1242, 1371, 2654, 2830
RseI CAYNNNNRTG 5 cut(s) 455, 626, 650, 1071, 2428
SacI GAGCTC 2 cut(s) 529, 2404
SalI GTCGAC 1 cut(s) 422
SapI GCTCTTC 2 cut(s) 1765, 2666
SaqAI TTAA 9 cut(s) 273, 986, 1796, 1829, 2106, 2576, 2583, 2913, 2965
SatI GCNGC 6 cut(s) 942, 1145, 1744, 2067, 2235, 2955
Sau3AI GATC 9 cut(s) 117, 266, 1063, 1261, 1919, 1993, 2122, 2134, 2213
Sau96I GGNCC 7 cut(s) 811, 954, 1227, 1322, 1323, 1501, 2614
ScaI AGTACT 1 cut(s) 1372
SchI GAGTC 3 cut(s) 844, 1822, 2180
ScrFI CCNGG 5 cut(s) 2071, 2072, 2087, 2484, 2594
SduI GDGCHC 7 cut(s) 30, 529, 591, 1326, 1534, 2404, 2766
SfcI CTRYAG 5 cut(s) 320, 995, 1583, 1851, 2168
SinI GGWCC 3 cut(s) 811, 954, 1227
SmaI CCCGGG 1 cut(s) 2072
SmiMI CAYNNNNRTG 5 cut(s) 455, 626, 650, 1071, 2428
SmlI CTYRAG 1 cut(s) 501
SmoI CTYRAG 1 cut(s) 501
SrfI GCCCGGGC 1 cut(s) 2072
SseBI AGGCCT 1 cut(s) 474
SsiI CCGC 6 cut(s) 30, 36, 397, 1680, 2075, 2539
SstI GAGCTC 2 cut(s) 529, 2404
StuI AGGCCT 1 cut(s) 474
StyD4I CCNGG 5 cut(s) 2069, 2070, 2085, 2482, 2592
StyI CCWWGG 5 cut(s) 1577, 1989, 2751, 2805, 2847
TaiI ACGT 4 cut(s) 535, 541, 1525, 2042
TaqI TCGA 6 cut(s) 14, 77, 423, 602, 907, 1996
TatI WGTACW 2 cut(s) 1241, 1370
TfiI GAWTC 8 cut(s) 364, 400, 698, 746, 1013, 2015, 2597, 2816
Tru1I TTAA 9 cut(s) 273, 986, 1796, 1829, 2106, 2576, 2583, 2913, 2965
Tru9I TTAA 9 cut(s) 273, 986, 1796, 1829, 2106, 2576, 2583, 2913, 2965
TscAI CASTG 5 cut(s) 69, 564, 633, 716, 906
TseFI GTSAC 3 cut(s) 628, 999, 1114
TseI GCWGC 6 cut(s) 941, 1144, 1743, 2066, 2234, 2954
Tsp45I GTSAC 3 cut(s) 628, 999, 1114
TspGWI ACGGA 3 cut(s) 662, 699, 828
TspMI CCCGGG 1 cut(s) 2070
TspRI CASTG 5 cut(s) 69, 564, 633, 716, 906
VpaK11BI GGWCC 3 cut(s) 811, 954, 1227
VspI ATTAAT 1 cut(s) 1829
XapI RAATTY 7 cut(s) 201, 931, 1870, 2312, 2453, 2856, 2942
XbaI TCTAGA 2 cut(s) 223, 2605
XceI RCATGY 2 cut(s) 460, 2056
XmaI CCCGGG 1 cut(s) 2070
XmiI GTMKAC 3 cut(s) 346, 423, 535
ZrmI AGTACT 1 cut(s) 1372
Zsp2I ATGCAT 3 cut(s) 657, 1289, 2389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.