Rh4AG141400

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0003224
Physical Location & Seq
Forward (+)
35880840 .. 35893869
13030 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG141400.1

Sequence Viewer

Length: 2595 bp
ATGGGGGTTGTATCGAGGCGAGTTGTGCCCGCCTGCGGTAGCCTCTGTTTCTTCTGCCCCTCACTGCGTGCAAGGTCGAGGCAACCAGTCAAACGCTACAAGAAGTTGCTCGCTGAGATCTTCCCCCGTAATCAGGATGCTGAACCCAATGACAGAAAGATTGGGAAACTTTGTGAATATGCTCTGAAGAACCCATTGCGAATTCCCAAGATTACTGAGCATCTAGAGCAAAAATGTTACAAGGATTTGCGAAACGAACAGTTTGGATCTGTTAAAGTCATACTGTGCATTTATAGGAAGTTACTATCTTCCTGTAAAGTGCAGATGCCACTATTTGCAAGTAGTCTACTAGGAATTGTTCGGATTCTCTTAGAACAAACCCGACATGATGAAATGCGGGTTCTGGGTTGCAAAACACTTGTCGACTTTATAAATAGCCAGATTGATAGCACACACATGTTCAACCTAGAAGGCCTTATTCCTAAACTCTGTGAATTGGCTCAAGAAATGGGAGATGATGAAAGAGCTCTACGTCTACGTTCAGCTGGATTGCAATCACTGGCTTTTATGGTGTGGTTCATGGGTGAGCACTCTCATATCTCGATGGATTTTGACAATATCATATCAGTGACTTTGGAAAATTACACGGATATGCATACTAAACCTGGGAGTGTGAAAGAAGACGGACAATTTTCTGAATCTCAGCACCAGTGGGTTCAAGAAGCGCTAAAAGCAGAAGTTCATGATTCATCTTTTCCAGATGTTAGTCAGAAGGTTCCTTCATTACCAAACCTGAAAACCCCTGATTTGGACCCCACAATTGATACAGACAAGAGTCCGTCTTACTGGTCAAAGGTTTGCTTGCGTAATATAGCCAGATTGGCAAAGGAAGCTACAACAGTGCGTCGAGTGCTTGAACCCCTTTTCCAAAATTTTGATGCTGCGAACCATTGGTCCCCAGAAAAACCACTTGCCTATTATGTTTTAATGTATCTGCAGTCACTATTAGAGGAATCAGGAGATAACTCTCATCTGTTATTATCCATATTGGTCAAGCACTTGGATCATAAGAATGTTGTCAAACAGCCTCGCTTGCAAACTGATATTGTAAATGTCACCACACAAATAGCACAAAGTGCAAAGCAGCAGGCATCAGTTGCGATTATTGGTGCAATATCTGACCTGATAAAGCATTTACGAAAATGCCTGCAAAATCAAGCTGAAGGGTGCAACCGTAGTAGTACAGATAAATGGAACCAAGATCTTCAGTCAGCACTAGAGAGATGCATCTCGCAACTGTCAAATAAGGTTGGAGATGTGGGGCCCATACTTGATATGATGGCTGTGGTGCTGGAGAATATCCCATCTAGTACTATTGTAGCTAGGGCGACGGTCTCTGCTGTTTATGTAACTGCAAAGATGGTCTCTTCTGTTCCAAACATATCCTATCACAAAAAGCCATTCCCTGATGCTCTGTTTCACCAGTTACTCCTAGCAATGGCCCACCTAGACCATGAAACACGTGTTGGAGCACACAGTATTTTCTCTATTGTTCTTGTGCCATCTCTACTTGTTCCTTGGCTACAGCAGAAAATGAACTCTGTGCAGACTGTTTCTGGGTTTTCATCTGTCAGTAGATTGGACTTTGGAAAGGATGGGAGTTTCTCCATTAAGGATAAAAGAAAAGACACGGGAGCAACCGCGAATGGAGAGTTGAGGGAAGAGGAAAGTCAAATATCAGATGTTTGTGAAAATCAATCTGGCAGCTTCAAGAGTGCTTTGACGGGTGGAAGAGCAGAACTACCTTGCCTTCGGTTAAGCAGTCATCAAGTGAGTCTATTGCTTTCATTAATCTGGGTTCAGGCAACATCTGCAGAGAATACCCCAGCAAATTTTGAGGCGATGGCTCATTCTTATAATGTTGCTTTACTGTTTACCCGATCAAAGGCTTCAAGTCATATGGCTTTAGTAAGATGCTTCCAGTTGGCATTCTCCATCAGGACTATTTCCTTGGATCAAGATGGGGGTTTGCAAGAATCTCGTAGAAGGTCTCTTTATACGTTGGCATCATACATGCTTATATTTGCAGCCCGGGCGGGGAACTTCCCGGAGCTAATTCCCATTGTTAAAGCATCATTGATGGATCAAATGGTTGATCCTTGTCTTCAGTTGGTTGATGATATCCGACTGCAGGTAGTTTCAATTGACTCCGATATGGAGAAGCTACCCTATGGATCACAGCCAGACGAAGTTGCTGCATTGAAGTCTCATTCAGCAATTGAACTAGATGACCAATTGTTGAAAGAAACCGTGATATCCCACTTCATGACTAAATTTTCCATATTGTCAGAGGATGAATTGTCTAGCATCAAGAAGCAACTCTTACTGGGTTTTTCACCGGATGATGCATTTCCATTAGGAGCTCCACTATTCATGGAGACACCACGACCATGTTCTCCACTTGCCCATATAGATTTTGCTGATTTTGATGAGGTTAAATTTCTTAATTGTATCAGTATTTTCATCACTTTTAACTACATTTATAACAGAGGTTGTTTCCTCTCAGGTGATGCCTCCAGGTTCTTTGACAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

864

Amino Acids

96.8

Weight (kDa)

6.57

Isoelectric Point (pI)

46.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EFR3_ARM PF21052 65 - 151 9e-18 EFR3, armadillo repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 431, 1917, 2544
Acc36I ACCTGC 1 cut(s) 2182
AccI GTMKAC 3 cut(s) 346, 423, 535
AccII CGCG 1 cut(s) 1703
AciI CCGC 5 cut(s) 30, 36, 397, 1701, 2096
AclWI GGATC 6 cut(s) 274, 1071, 2022, 2150, 2151, 2242
AcsI RAATTY 5 cut(s) 201, 931, 1891, 2333, 2498
AcuI CTGAAG 4 cut(s) 206, 1242, 1250, 2150
AcvI CACGTG 1 cut(s) 1523
AdeI CACNNNGTG 2 cut(s) 68, 1136
AfaI GTAC 2 cut(s) 1243, 1372
AfeI AGCGCT 1 cut(s) 726
AfiI CCNNNNNNNGG 7 cut(s) 35, 133, 808, 1498, 1945, 2097, 2191
AflIII ACRYGT 3 cut(s) 456, 1520, 1522
AgsI TTSAA 9 cut(s) 463, 719, 917, 1771, 1953, 2202, 2263, 2282, 2302
AjnI CCWGG 2 cut(s) 664, 2576
AjuI GAANNNNNNNTTGG 2 cut(s) 1509, 1541
AloI GAACNNNNNNTCC 4 cut(s) 909, 938, 941, 970
AluBI AGCT 9 cut(s) 527, 545, 893, 1220, 1382, 1767, 2113, 2224, 2423
AluI AGCT 9 cut(s) 527, 545, 893, 1220, 1382, 1767, 2113, 2224, 2423
Alw21I GWGCWC 4 cut(s) 529, 591, 1534, 2425
Alw26I GTCTC 5 cut(s) 1399, 1429, 2055, 2271, 2432
AlwI GGATC 6 cut(s) 274, 1071, 2022, 2150, 2151, 2242
Ama87I CYCGRG 1 cut(s) 2091
Aor51HI AGCGCT 1 cut(s) 726
AoxI GGCC 3 cut(s) 472, 1322, 1500
ApaI GGGCCC 1 cut(s) 1326
ApeKI GCWGC 5 cut(s) 941, 1144, 1764, 2087, 2255
ApoI RAATTY 5 cut(s) 201, 931, 1891, 2333, 2498
ArsI GACNNNNNNTTYG 4 cut(s) 405, 437, 938, 970
AseI ATTAAT 1 cut(s) 1850
AspLEI GCGC 1 cut(s) 727
AspS9I GGNCC 5 cut(s) 811, 954, 1322, 1323, 1501
AsuC2I CCSGG 3 cut(s) 2092, 2093, 2108
AsuHPI GGTGA 5 cut(s) 596, 1108, 1472, 2388, 2579
AvaI CYCGRG 1 cut(s) 2091
AvaII GGWCC 2 cut(s) 811, 954
BaeGI GKGCMC 2 cut(s) 30, 1326
BanII GRGCYC 3 cut(s) 529, 1326, 2425
BbrPI CACGTG 1 cut(s) 1523
BbsI GAAGAC 2 cut(s) 687, 2156
Bbv12I GWGCWC 4 cut(s) 529, 591, 1534, 2425
BbvI GCAGC 5 cut(s) 928, 1156, 1776, 2099, 2242
BcgI CGANNNNNNTGC 4 cut(s) 2021, 2055, 2237, 2271
BciT130I CCWGG 2 cut(s) 666, 2578
BcnI CCSGG 3 cut(s) 2092, 2093, 2108
BcoDI GTCTC 5 cut(s) 1399, 1429, 2055, 2271, 2432
BfmI CTRYAG 4 cut(s) 995, 1583, 1872, 2189
BfoI RGCGCY 1 cut(s) 728
BfuAI ACCTGC 1 cut(s) 2182
BglI GCCNNNNNGGC 1 cut(s) 881
BglII AGATCT 2 cut(s) 117, 1261
BisI GCNGC 5 cut(s) 942, 1145, 1765, 2088, 2256
BlsI GCNGC 5 cut(s) 943, 1146, 1766, 2089, 2257
BmcAI AGTACT 1 cut(s) 1372
Bme1390I CCNGG 5 cut(s) 666, 2092, 2093, 2108, 2578
Bme18I GGWCC 2 cut(s) 811, 954
BmeT110I CYCGRG 1 cut(s) 2091
BmgT120I GGNCC 5 cut(s) 811, 954, 1322, 1323, 1501
BmiI GGNNCC 6 cut(s) 777, 813, 956, 1256, 1323, 1324
BmrFI CCNGG 5 cut(s) 666, 2092, 2093, 2108, 2578
BmrI ACTGGG 1 cut(s) 2396
BmuI ACTGGG 1 cut(s) 2396
BoxI GACNNNNGTC 1 cut(s) 834
BpiI GAAGAC 2 cut(s) 687, 2156
BpmI CTGGAG 2 cut(s) 1373, 2560
BpuEI CTTGAG 1 cut(s) 486
BpuMI CCSGG 3 cut(s) 2092, 2093, 2108
BsaAI YACGTR 1 cut(s) 1523
BsaBI GATNNNNATC 1 cut(s) 553
BsaI GGTCTC 3 cut(s) 1399, 1429, 2055
BsaJI CCNNGG 4 cut(s) 665, 1577, 2010, 2091
BsaWI WCCGGW 1 cut(s) 2398
Bsc4I CCNNNNNNNGG 7 cut(s) 35, 133, 808, 1498, 1945, 2097, 2191
Bse1I ACTGG 7 cut(s) 86, 564, 709, 851, 1483, 1981, 2391
Bse3DI GCAATG 2 cut(s) 194, 1503
Bse8I GATNNNNATC 1 cut(s) 553
BseBI CCWGG 2 cut(s) 666, 2578
BseDI CCNNGG 4 cut(s) 665, 1577, 2010, 2091
BseGI GGATG 4 cut(s) 142, 1660, 2359, 2407
BseJI GATNNNNATC 1 cut(s) 553
BseLI CCNNNNNNNGG 7 cut(s) 35, 133, 808, 1498, 1945, 2097, 2191
BseMI GCAATG 2 cut(s) 194, 1503
BseMII CTCAG 4 cut(s) 105, 207, 716, 2577
BseNI ACTGG 7 cut(s) 86, 564, 709, 851, 1483, 1981, 2391
BseSI GKGCMC 2 cut(s) 30, 1326
BseXI GCAGC 5 cut(s) 928, 1156, 1776, 2099, 2242
BseYI CCCAGC 1 cut(s) 1885
BsgI GTGCAG 2 cut(s) 341, 1625
Bsh1236I CGCG 1 cut(s) 1703
BshFI GGCC 3 cut(s) 474, 1324, 1502
BsiHKAI GWGCWC 4 cut(s) 529, 591, 1534, 2425
BsiHKCI CYCGRG 1 cut(s) 2091
BsiSI CCGG 3 cut(s) 2092, 2108, 2399
BslFI GGGAC 1 cut(s) 940
BslI CCNNNNNNNGG 7 cut(s) 35, 133, 808, 1498, 1945, 2097, 2191
BsmAI GTCTC 5 cut(s) 1399, 1429, 2055, 2271, 2432
BsmFI GGGAC 1 cut(s) 940
BsmI GAATGC 1 cut(s) 1988
BsnI GGCC 3 cut(s) 474, 1324, 1502
Bso31I GGTCTC 3 cut(s) 1399, 1429, 2055
BsoBI CYCGRG 1 cut(s) 2091
Bsp120I GGGCCC 1 cut(s) 1322
Bsp1286I GDGCHC 6 cut(s) 30, 529, 591, 1326, 1534, 2425
Bsp143I GATC 9 cut(s) 117, 266, 1063, 1261, 1940, 2014, 2143, 2155, 2234
BspACI CCGC 5 cut(s) 30, 36, 397, 1701, 2096
BspANI GGCC 3 cut(s) 474, 1324, 1502
BspCNI CTCAG 4 cut(s) 106, 208, 715, 2576
BspFNI CGCG 1 cut(s) 1703
BspHI TCATGA 2 cut(s) 742, 2325
BspLI GGNNCC 6 cut(s) 777, 813, 956, 1256, 1323, 1324
BspMAI CTGCAG 3 cut(s) 999, 1876, 2193
BspMI ACCTGC 1 cut(s) 2182
BspPI GGATC 6 cut(s) 274, 1071, 2022, 2150, 2151, 2242
BspQI GCTCTTC 1 cut(s) 1786
BspTNI GGTCTC 3 cut(s) 1399, 1429, 2055
BsrDI GCAATG 2 cut(s) 194, 1503
BsrI ACTGG 7 cut(s) 86, 564, 709, 851, 1483, 1981, 2391
BssECI CCNNGG 4 cut(s) 665, 1577, 2010, 2091
BssMI GATC 9 cut(s) 117, 266, 1063, 1261, 1940, 2014, 2143, 2155, 2234
BssT1I CCWWGG 2 cut(s) 1577, 2010
Bst2UI CCWGG 2 cut(s) 666, 2578
Bst6I CTCTTC 3 cut(s) 1432, 1716, 1786
BstAPI GCANNNNNTGC 3 cut(s) 1136, 1157, 1871
BstBAI YACGTR 1 cut(s) 1523
BstC8I GCNNGC 8 cut(s) 30, 34, 69, 111, 863, 1094, 1149, 1208
BstDEI CTNAG 5 cut(s) 114, 216, 370, 702, 2563
BstF5I GGATG 4 cut(s) 142, 1660, 2359, 2407
BstFNI CGCG 1 cut(s) 1703
BstH2I RGCGCY 1 cut(s) 728
BstHHI GCGC 1 cut(s) 727
BstKTI GATC 9 cut(s) 120, 269, 1066, 1264, 1943, 2017, 2146, 2158, 2237
BstMAI GTCTC 5 cut(s) 1399, 1429, 2055, 2271, 2432
BstMBI GATC 9 cut(s) 117, 266, 1063, 1261, 1940, 2014, 2143, 2155, 2234
BstNI CCWGG 2 cut(s) 666, 2578
BstNSI RCATGY 2 cut(s) 460, 2077
BstPAI GACNNNNGTC 1 cut(s) 834
BstSCI CCNGG 5 cut(s) 664, 2090, 2091, 2106, 2576
BstSFI CTRYAG 4 cut(s) 995, 1583, 1872, 2189
BstSLI GKGCMC 2 cut(s) 30, 1326
BstUI CGCG 1 cut(s) 1703
BstV1I GCAGC 5 cut(s) 928, 1156, 1776, 2099, 2242
BstV2I GAAGAC 2 cut(s) 687, 2156
BstX2I RGATCY 3 cut(s) 117, 266, 1261
BstYI RGATCY 3 cut(s) 117, 266, 1261
BsuRI GGCC 3 cut(s) 474, 1324, 1502
BtgZI GCGATG 1 cut(s) 1916
BtsCI GGATG 4 cut(s) 142, 1660, 2359, 2407
BtsI GCAGTG 1 cut(s) 62
BtsIMutI CAGTG 5 cut(s) 62, 557, 633, 716, 906
BveI ACCTGC 1 cut(s) 2182
Cac8I GCNNGC 8 cut(s) 30, 34, 69, 111, 863, 1094, 1149, 1208
CciI TCATGA 2 cut(s) 742, 2325
CfoI GCGC 1 cut(s) 727
Cfr13I GGNCC 5 cut(s) 811, 954, 1322, 1323, 1501
Cfr9I CCCGGG 1 cut(s) 2091
CseI GACGC 1 cut(s) 893
Csp6I GTAC 2 cut(s) 1242, 1371
CviAII CATG 9 cut(s) 386, 457, 580, 743, 1514, 2074, 2326, 2434, 2451
CviQI GTAC 2 cut(s) 1242, 1371
DdeI CTNAG 5 cut(s) 114, 216, 370, 702, 2563
DpnI GATC 9 cut(s) 119, 268, 1065, 1263, 1942, 2016, 2145, 2157, 2236
DpnII GATC 9 cut(s) 117, 266, 1063, 1261, 1940, 2014, 2143, 2155, 2234
DraIII CACNNNGTG 2 cut(s) 68, 1136
Eam1104I CTCTTC 3 cut(s) 1432, 1716, 1786
EarI CTCTTC 3 cut(s) 1432, 1716, 1786
Ecl136II GAGCTC 2 cut(s) 527, 2423
Eco130I CCWWGG 2 cut(s) 1577, 2010
Eco147I AGGCCT 1 cut(s) 474
Eco24I GRGCYC 3 cut(s) 529, 1326, 2425
Eco31I GGTCTC 3 cut(s) 1399, 1429, 2055
Eco32I GATATC 2 cut(s) 2182, 2316
Eco47I GGWCC 2 cut(s) 811, 954
Eco47III AGCGCT 1 cut(s) 726
Eco53kI GAGCTC 2 cut(s) 527, 2423
Eco57I CTGAAG 4 cut(s) 206, 1242, 1250, 2150
Eco72I CACGTG 1 cut(s) 1523
Eco88I CYCGRG 1 cut(s) 2091
EcoICRI GAGCTC 2 cut(s) 527, 2423
EcoO109I RGGNCCY 1 cut(s) 1322
EcoRI GAATTC 1 cut(s) 201
EcoRII CCWGG 2 cut(s) 664, 2576
EcoRV GATATC 2 cut(s) 2182, 2316
EcoT14I CCWWGG 2 cut(s) 1577, 2010
EcoT22I ATGCAT 3 cut(s) 657, 1289, 2410
EcoT38I GRGCYC 3 cut(s) 529, 1326, 2425
ErhI CCWWGG 2 cut(s) 1577, 2010
FaeI CATG 9 cut(s) 389, 460, 583, 746, 1517, 2077, 2329, 2437, 2454
FalI AAGNNNNNCTT 4 cut(s) 845, 877, 2366, 2398
FaqI GGGAC 1 cut(s) 940
FatI CATG 9 cut(s) 385, 456, 579, 742, 1513, 2073, 2325, 2433, 2450
FauI CCCGC 3 cut(s) 37, 390, 2089
FauNDI CATATG 1 cut(s) 1959
FblI GTMKAC 3 cut(s) 346, 423, 535
Fnu4HI GCNGC 5 cut(s) 942, 1145, 1765, 2088, 2256
FokI GGATG 4 cut(s) 149, 1667, 2366, 2414
FriOI GRGCYC 3 cut(s) 529, 1326, 2425
Fsp4HI GCNGC 5 cut(s) 942, 1145, 1765, 2088, 2256
GlaI GCGC 1 cut(s) 726
GluI GCNGC 5 cut(s) 942, 1145, 1765, 2088, 2256
GsaI CCCAGC 1 cut(s) 1889
GsuI CTGGAG 2 cut(s) 1373, 2560
HaeII RGCGCY 1 cut(s) 728
HaeIII GGCC 3 cut(s) 474, 1324, 1502
HapII CCGG 3 cut(s) 2092, 2108, 2399
HgaI GACGC 1 cut(s) 893
HhaI GCGC 1 cut(s) 727
Hin1II CATG 9 cut(s) 389, 460, 583, 746, 1517, 2077, 2329, 2437, 2454
Hin6I GCGC 1 cut(s) 725
HinP1I GCGC 1 cut(s) 725
HincII GTYRAC 1 cut(s) 424
HindII GTYRAC 1 cut(s) 424
HinfI GANTC 8 cut(s) 364, 698, 746, 835, 1013, 1834, 2036, 2207
HpaII CCGG 3 cut(s) 2092, 2108, 2399
HphI GGTGA 5 cut(s) 596, 1108, 1472, 2388, 2579
Hpy166II GTNNAC 4 cut(s) 347, 424, 536, 1935
Hpy188I TCNGA 9 cut(s) 186, 363, 697, 771, 1180, 1741, 2186, 2212, 2350
Hpy8I GTNNAC 4 cut(s) 347, 424, 536, 1935
Hpy99I CGWCG 2 cut(s) 909, 1393
HpyAV CCTTC 6 cut(s) 464, 766, 789, 1217, 1820, 2040
HpyCH4IV ACGT 4 cut(s) 532, 538, 1522, 2060
HpyF3I CTNAG 5 cut(s) 114, 216, 370, 702, 2563
HpySE526I ACGT 4 cut(s) 532, 538, 1522, 2060
Hsp92II CATG 9 cut(s) 389, 460, 583, 746, 1517, 2077, 2329, 2437, 2454
HspAI GCGC 1 cut(s) 725
Kzo9I GATC 9 cut(s) 117, 266, 1063, 1261, 1940, 2014, 2143, 2155, 2234
LguI GCTCTTC 1 cut(s) 1786
LmnI GCTCC 5 cut(s) 1529, 1694, 2110, 2420, 2428
Lsp1109I GCAGC 5 cut(s) 928, 1156, 1776, 2099, 2242
MaeII ACGT 4 cut(s) 532, 538, 1522, 2060
MaeIII GTNAC 7 cut(s) 236, 300, 628, 999, 1114, 1408, 1485
MalI GATC 9 cut(s) 119, 268, 1065, 1263, 1942, 2016, 2145, 2157, 2236
MboI GATC 9 cut(s) 117, 266, 1063, 1261, 1940, 2014, 2143, 2155, 2234
MfeI CAATTG 4 cut(s) 819, 2202, 2277, 2294
MflI RGATCY 3 cut(s) 117, 266, 1261
MhlI GDGCHC 6 cut(s) 30, 529, 591, 1326, 1534, 2425
MlyI GAGTC 3 cut(s) 844, 1843, 2201
MmeI TCCRAC 3 cut(s) 1291, 1507, 2209
Mph1103I ATGCAT 3 cut(s) 657, 1289, 2410
MseI TTAA 9 cut(s) 273, 986, 1671, 1817, 1850, 2127, 2496, 2505, 2532
MslI CAYNNNNRTG 5 cut(s) 455, 626, 650, 1071, 2449
MspA1I CMGCKG 1 cut(s) 545
MspI CCGG 3 cut(s) 2092, 2108, 2399
MspR9I CCNGG 5 cut(s) 666, 2092, 2093, 2108, 2578
MunI CAATTG 4 cut(s) 819, 2202, 2277, 2294
Mva1269I GAATGC 1 cut(s) 1988
MvaI CCWGG 2 cut(s) 666, 2578
MvnI CGCG 1 cut(s) 1703
NciI CCSGG 3 cut(s) 2092, 2093, 2108
NdeI CATATG 1 cut(s) 1959
NdeII GATC 9 cut(s) 117, 266, 1063, 1261, 1940, 2014, 2143, 2155, 2234
NlaIII CATG 9 cut(s) 389, 460, 583, 746, 1517, 2077, 2329, 2437, 2454
NlaIV GGNNCC 6 cut(s) 777, 813, 956, 1256, 1323, 1324
NmuCI GTSAC 3 cut(s) 628, 999, 1114
NsiI ATGCAT 3 cut(s) 657, 1289, 2410
NspI RCATGY 2 cut(s) 460, 2077
PagI TCATGA 2 cut(s) 742, 2325
PceI AGGCCT 1 cut(s) 474
PciI ACATGT 1 cut(s) 456
PciSI GCTCTTC 1 cut(s) 1786
PctI GAATGC 1 cut(s) 1988
PfeI GAWTC 5 cut(s) 364, 698, 746, 1013, 2036
PfoI TCCNGGA 1 cut(s) 2106
PkrI GCNGC 5 cut(s) 943, 1146, 1766, 2089, 2257
PleI GAGTC 3 cut(s) 843, 1842, 2201
PmaCI CACGTG 1 cut(s) 1523
PmlI CACGTG 1 cut(s) 1523
PpsI GAGTC 3 cut(s) 843, 1842, 2201
Ppu21I YACGTR 1 cut(s) 1523
PscI ACATGT 1 cut(s) 456
PshAI GACNNNNGTC 1 cut(s) 834
PshBI ATTAAT 1 cut(s) 1850
PsiI TTATAA 3 cut(s) 431, 1917, 2544
Psp124BI GAGCTC 2 cut(s) 529, 2425
Psp6I CCWGG 2 cut(s) 664, 2576
PspCI CACGTG 1 cut(s) 1523
PspFI CCCAGC 1 cut(s) 1885
PspGI CCWGG 2 cut(s) 664, 2576
PspN4I GGNNCC 6 cut(s) 777, 813, 956, 1256, 1323, 1324
PspOMI GGGCCC 1 cut(s) 1322
PspPI GGNCC 5 cut(s) 811, 954, 1322, 1323, 1501
PstI CTGCAG 3 cut(s) 999, 1876, 2193
PsuI RGATCY 3 cut(s) 117, 266, 1261
PvuII CAGCTG 1 cut(s) 545
RsaI GTAC 2 cut(s) 1243, 1372
RsaNI GTAC 2 cut(s) 1242, 1371
RseI CAYNNNNRTG 5 cut(s) 455, 626, 650, 1071, 2449
SacI GAGCTC 2 cut(s) 529, 2425
SalI GTCGAC 1 cut(s) 422
SapI GCTCTTC 1 cut(s) 1786
SaqAI TTAA 9 cut(s) 273, 986, 1671, 1817, 1850, 2127, 2496, 2505, 2532
SatI GCNGC 5 cut(s) 942, 1145, 1765, 2088, 2256
Sau3AI GATC 9 cut(s) 117, 266, 1063, 1261, 1940, 2014, 2143, 2155, 2234
Sau96I GGNCC 5 cut(s) 811, 954, 1322, 1323, 1501
ScaI AGTACT 1 cut(s) 1372
SchI GAGTC 3 cut(s) 844, 1843, 2201
ScrFI CCNGG 5 cut(s) 666, 2092, 2093, 2108, 2578
SduI GDGCHC 6 cut(s) 30, 529, 591, 1326, 1534, 2425
SfcI CTRYAG 4 cut(s) 995, 1583, 1872, 2189
SinI GGWCC 2 cut(s) 811, 954
SmaI CCCGGG 1 cut(s) 2093
SmiMI CAYNNNNRTG 5 cut(s) 455, 626, 650, 1071, 2449
SmlI CTYRAG 1 cut(s) 501
SmoI CTYRAG 1 cut(s) 501
SrfI GCCCGGGC 1 cut(s) 2093
SseBI AGGCCT 1 cut(s) 474
SsiI CCGC 5 cut(s) 30, 36, 397, 1701, 2096
SstI GAGCTC 2 cut(s) 529, 2425
StuI AGGCCT 1 cut(s) 474
StyD4I CCNGG 5 cut(s) 664, 2090, 2091, 2106, 2576
StyI CCWWGG 2 cut(s) 1577, 2010
TaiI ACGT 4 cut(s) 535, 541, 1525, 2063
TaqI TCGA 5 cut(s) 14, 77, 423, 602, 907
TatI WGTACW 2 cut(s) 1241, 1370
TfiI GAWTC 5 cut(s) 364, 698, 746, 1013, 2036
Tru1I TTAA 9 cut(s) 273, 986, 1671, 1817, 1850, 2127, 2496, 2505, 2532
Tru9I TTAA 9 cut(s) 273, 986, 1671, 1817, 1850, 2127, 2496, 2505, 2532
TscAI CASTG 5 cut(s) 69, 564, 633, 716, 906
TseFI GTSAC 3 cut(s) 628, 999, 1114
TseI GCWGC 5 cut(s) 941, 1144, 1764, 2087, 2255
Tsp45I GTSAC 3 cut(s) 628, 999, 1114
TspGWI ACGGA 3 cut(s) 662, 699, 828
TspMI CCCGGG 1 cut(s) 2091
TspRI CASTG 5 cut(s) 69, 564, 633, 716, 906
VpaK11BI GGWCC 2 cut(s) 811, 954
VspI ATTAAT 1 cut(s) 1850
XapI RAATTY 5 cut(s) 201, 931, 1891, 2333, 2498
XbaI TCTAGA 1 cut(s) 223
XceI RCATGY 2 cut(s) 460, 2077
XmaI CCCGGG 1 cut(s) 2091
XmiI GTMKAC 3 cut(s) 346, 423, 535
ZrmI AGTACT 1 cut(s) 1372
Zsp2I ATGCAT 3 cut(s) 657, 1289, 2410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.